LOCUS       NZ_CP115936            11299 bp    DNA     linear   CON 13-JUL-2024
DEFINITION  Streptomyces goshikiensis strain CGMCC 4.1796 chromosome, complete
            genome.
ACCESSION   NZ_CP115936
VERSION     NZ_CP115936.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces goshikiensis
  ORGANISM  Streptomyces goshikiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 7239828)
  AUTHORS   Mo,P.
  TITLE     The genome sequence of Streptomyces sporoverrucosus CGMCC 4.1796T
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7239828)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 184.03x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027942015.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 07:01:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,266
            CDSs (total)                      :: 7,170
            Genes (coding)                    :: 7,009
            CDSs (with protein)               :: 7,009
            Genes (RNA)                       :: 96
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 55 of 162
            Pseudo Genes (incomplete)         :: 131 of 162
            Pseudo Genes (internal stop)      :: 14 of 162
            Pseudo Genes (multiple problems)  :: 36 of 162
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:49:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5667757
            Orig. end                         :: 5679056
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115936.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Mo Ping.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11299
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="14"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11299
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11299
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6299](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6299)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1305..2018
                     /db_xref="GeneID:91412242"
                     /gene="hemQ"
                     /locus_tag="PET44_RS25090"
                     /old_locus_tag="PET44_25090"
     CDS             1305..2018
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /db_xref="GeneID:91412242"
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072526.1"
                     /locus_tag="PET44_RS25090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25090"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_037798306.1"
                     /transl_table=11
                     /translation="MTAPEKIPNAGKKAKDLNEVIRYTLWSVFKLKDVLPQDRAGFADE
                     VQELFDQLAAKDITVRGTYDVSGLRADADIMIWWHAETSDELQTAYNLFRRTRLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADEVERDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVADLVDGLA"
     gene            complement(2133..3794)
                     /db_xref="GeneID:91412243"
                     /locus_tag="PET44_RS25095"
                     /old_locus_tag="PET44_25095"
     CDS             complement(2133..3794)
                     /NRPS_PKS="Domain: Abhydrolase_1 (111-479). E-value:
                     1.6e-23. Score: 75.5. Matches aSDomain:
                     nrpspksdomains_PET44_RS25095_Abhydrolase_1.1"
                     /codon_start=1
                     /db_xref="GeneID:91412243"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888542.1"
                     /locus_tag="PET44_RS25095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25095"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_271244715.1"
                     /sec_met_domain="PF00561 (E-value: 2.2e-29, bitscore:
                     101.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRMPLVRPAVAATALAFALTLTLAPPAPASAPATAVAAENAGAER
                     VARRAAAGHRALDFRSCPAAEGLAAPVRCAALRVPLDYARPDGPQISLTVSRVGATREG
                     RAARQGSLVFNPGGPGASGMYFPLLADRPAWRRVAAAYDLVGYAPRGVGRSAPLSCQDP
                     AARPKAPTQVPADPSAAYKRERVAAARAYARGCARRGGAALPYYTTLNNARDLHVLRAA
                     LGERELTFVGASYGTYLGAVYATLYPGHVRRMVFDSAVDPDPRHVWYRDNLAQAAGFER
                     RWYDFRAWAARHHATYRLGATPAAVRASHERVRAAVARTPAGGVAGTGELRAAALRAAY
                     YDEVWPELAAALSAFLDGDPAPLAALAGPDPDPVAAARAAAEAENATALYTAVLCNDAP
                     WPADWETWDRDHTELARRAPLETWANAFLNLPCAYWPVAQRLRQRPVDVGSRATPLPPT
                     LIVAAERDGATPYPGALELRRRLGGAAALVTEAGAGAHGVAGGRNDCVDRHVERYILTG
                     DTPKGSVTCAPHPEPAPVSLDDRAAGTRRVPLPPVV"
     aSDomain        complement(2358..3461)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PET44_RS25095_Abhydrolase_1.1"
                     /evalue="1.60E-23"
                     /label="PET44_RS25095_Abhydrolase_1.1"
                     /locus_tag="PET44_RS25095"
                     /protein_end="479"
                     /protein_start="111"
                     /score="75.5"
                     /tool="antismash"
                     /translation="LVFNPGGPGASGMYFPLLADRPAWRRVAAAYDLVGYAPRGVGRSA
                     PLSCQDPAARPKAPTQVPADPSAAYKRERVAAARAYARGCARRGGAALPYYTTLNNARD
                     LHVLRAALGERELTFVGASYGTYLGAVYATLYPGHVRRMVFDSAVDPDPRHVWYRDNLA
                     QAAGFERRWYDFRAWAARHHATYRLGATPAAVRASHERVRAAVARTPAGGVAGTGELRA
                     AALRAAYYDEVWPELAAALSAFLDGDPAPLAALAGPDPDPVAAARAAAEAENATALYTA
                     VLCNDAPWPADWETWDRDHTELARRAPLETWANAFLNLPCAYWPVAQRLRQRPVDVGSR
                     ATPLPPTLIVAAERDGATPYPGALELRR"
     gene            complement(3907..5004)
                     /db_xref="GeneID:91412244"
                     /locus_tag="PET44_RS25100"
                     /old_locus_tag="PET44_25100"
     CDS             complement(3907..5004)
                     /codon_start=1
                     /db_xref="GeneID:91412244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100704.1"
                     /locus_tag="PET44_RS25100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25100"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_271244716.1"
                     /transl_table=11
                     /translation="MNLLAWAVWIAVITSTVLLLRGLRRSRPAPGGPAHPPLDLSEAAF
                     MAGGPGAVVDAALVSLLGDQRMVAGGPGIVQVRPGARAVDVAERAVLQAYANAPSGWLY
                     QVRYAAMTDPAIQEIGDALAERGLISPPGAARRWRRWGLAQAVLCGALLPVSLVLTFVG
                     FALEPGFQLPFLVKVFPVLIGGLVVGVAAAARAKRRITPAGRTALRAMRATHLTDQTPH
                     VQTALFGLRGLRDPYLREQLVPAARSTRLAAAQAGQRRSAGSPGSRSSWGSSSADALPV
                     VWCASVDGGSGGGSSGCGGSSGCGGSSGCGGSSGSGCGSSGSGCGSSGSGCGSSGSGCG
                     SSSSSCSSGGGSSCGSSSSSCSSSS"
     gene            complement(5001..6299)
                     /db_xref="GeneID:91412245"
                     /locus_tag="PET44_RS25105"
                     /old_locus_tag="PET44_25105"
     CDS             complement(5001..6299)
                     /codon_start=1
                     /db_xref="GeneID:91412245"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238120.1"
                     /locus_tag="PET44_RS25105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25105"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_271245182.1"
                     /sec_met_domain="DUF692 (E-value: 5.1e-90, bitscore: 299.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAHLGVGIGWRPEIADAVERLPGLDWVEVVAENICPGHLPASLLR
                     LRERGVRVVPHGVSLGLGGAHRPDPEKLAALGERAVALGAPLVTEHIAFVRTTTPAPAL
                     EAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALISWPGEEMTEGRFLSELV
                     ERTGVRLLIDVANLHTNRVNRGEDPAAVLDAIPLEALAYVHVAGGFERDGVWHDTHAHP
                     VPGVVLDVLAQLRRRVDPPGVLLERDDDFPPEGELAGELEAIRGVLDPAGSRPSASLRT
                     PSRHSAPEPAADPVPDAARTRVGLAQAALLSALVAGTPVPEGFDRQRIRVQSRSLAAKR
                     AGVVAKVAPELPGILGGEKAYREAFLAYARNRPMTGGYRRDALDFAEHLLIRELPADPA
                     DRRRLTAWWQERAGTRPARRIVRWARALVGRAA"
     gene            complement(6423..7178)
                     /db_xref="GeneID:91412246"
                     /locus_tag="PET44_RS25110"
                     /old_locus_tag="PET44_25110"
     CDS             complement(6423..7178)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91412246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267202.1"
                     /locus_tag="PET44_RS25110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25110"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_037799965.1"
                     /transl_table=11
                     /translation="MSSPDTHHDDSTPVPAAGAEHPPRPQRSARDEAPQFVLPLVVRIE
                     KAEPPARTDALETAARAVLVLLSDERALGEGEWAGAVRDWQDARIRKVVRRARGAEWRK
                     AETLPGVTVHGDAAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPVAPAPDGIAVLWL
                     NPDLEMSAGKAMAQAGHAAQLAWWELTGAERTAWRDSGFRLAVRTAPRERWARLSGSGL
                     PVVRDAGFTEIAPGSATVVSDHPALRARL"
     gene            7286..7960
                     /db_xref="GeneID:91412247"
                     /locus_tag="PET44_RS25115"
                     /old_locus_tag="PET44_25115"
     CDS             7286..7960
                     /codon_start=1
                     /db_xref="GeneID:91412247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014153329.1"
                     /locus_tag="PET44_RS25115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25115"
                     /product="AIM24 family protein"
                     /protein_id="WP_037798308.1"
                     /transl_table=11
                     /translation="MKSDLFASEHLAQPSAAPGMTLQNAKSVKYTVNGEMLARQGSMVA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMSVRGQGDAWFAHQAGNCFIIDFEPGDALT
                     INGRNVLCFDPTLSYEIKMVKGAGMTGGGLFNSVFTGTGKLAVVCDGHPIIIPVTAQNP
                     VYVDTDAVVGWSAQLETGLHRSQSVGSMIRGGSGEAVQLMLRGEGFVIVRPSELTETAA
                     AH"
     gene            8002..8817
                     /db_xref="GeneID:91412248"
                     /locus_tag="PET44_RS25120"
                     /old_locus_tag="PET44_25120"
     CDS             8002..8817
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91412248"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267204.1"
                     /locus_tag="PET44_RS25120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25120"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_271244717.1"
                     /transl_table=11
                     /translation="MRLDAVGRRYGVRGPWVLRGVSLELPPGALVRVEGRNGGGKSTLL
                     RLVAGIDTPTEGSITGRPRRTAYVPERFPAVLPLTAQAYLGHLGRVHGLTRAEAARRAA
                     QWLERFGAAGHARTPLAELSKGTSQKVAVAQALLAGPGLLVLDEAWTGLDVPARAELDR
                     AVAERTAAGGTVLFVDHDPARLAGAPDRHYRVEGGALEPVRPGAGPGPRVRIEAAGPPG
                     AVPPPGLAAEPLPAGGFRLTVPAGRSDAVLRELLTAHPAWHIRNVSDLP"
     gene            8814..9452
                     /db_xref="GeneID:91412249"
                     /locus_tag="PET44_RS25125"
                     /old_locus_tag="PET44_25125"
     CDS             8814..9452
                     /codon_start=1
                     /db_xref="GeneID:91412249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="PET44_RS25125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25125"
                     /product="ABC transporter"
                     /protein_id="WP_271244718.1"
                     /transl_table=11
                     /translation="MTALLRYQGALLLRSQRWLAPVLLYAAFLAVGFRPGDPVPDSLGY
                     SAAALVPVTAWLVRICLSVEPDAARDCAAAAAGPVRVHAAALLTGLGWGAGAGSLGAVA
                     VLLLGAREGPRLPAAALAGAASVLVCALTGAAVGALCARPLLRAPGYAVAAAGLGSLLA
                     LVTAGSPARVAVSGLVGGSVALPLGALAGAAALACAAGAAACLAAARRG"
     gene            9466..9657
                     /db_xref="GeneID:91412250"
                     /locus_tag="PET44_RS25130"
                     /old_locus_tag="PET44_25130"
     CDS             9466..9657
                     /codon_start=1
                     /db_xref="GeneID:91412250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997022.1"
                     /locus_tag="PET44_RS25130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25130"
                     /product="hypothetical protein"
                     /protein_id="WP_037798312.1"
                     /transl_table=11
                     /translation="MDQSEPTESYCPAPAAPARVVAGPPYADCLECGKPTEYGVRTPGV
                     VLCPVCEWQEAQRTACSG"
     gene            complement(9680..10612)
                     /db_xref="GeneID:91412251"
                     /locus_tag="PET44_RS25135"
                     /old_locus_tag="PET44_25135"
     CDS             complement(9680..10612)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:91412251"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378550.1"
                     /locus_tag="PET44_RS25135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="PET44_25135"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_037798315.1"
                     /transl_table=11
                     /translation="MTSSLRKAAAAAVLSGALGAVLAGCGGGAGPSNGGAGQARLGASA
                     TPSASASASARASAPAPAAGAASALPRAGAPGKAPTLAPGPGGLTPVFERARQKTEKTV
                     ALTFDADMTSDQGPRAAGGEHFDNPQLIDTLRALKVPSTVFMTGRWAEEYPDQAKAIGT
                     DPNFEIANHSYSHHAFKSPCYGLPTLDGAAARADVDRAFDAFRKAGAVNTVPYFRFPGG
                     CYDDQALRALSTAKVTAVQWDVVSGDAFAKDPDAVAEQVLAGVKPGSLVVMHCTRSAAP
                     VTEEAIRTIVPELRKRGYRFVKVSELMGT"
     misc_feature    complement(10617..10626)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 12.62"
                     /tool="antismash"
     misc_feature    complement(10620..10634)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.73"
                     /tool="antismash"
ORIGIN
        1 cccgtcgacc tcggcgccga atccgtgctc gcccgccgcc ccgaagccgt ggcgctcgcc
       61 cgggccgtcg gcctcggcga ggccctccag ccgcccgcca ccgccaccgc ccagctgtgg
      121 acccggggcg cgctgcggcc catgccgcgc ggccatgtca tgggcgtccc cggcgacttg
      181 gcgccgctcg ccgcctccgg ggtgctctcc gccgagggcc tggcccggat cgaggccgag
      241 cgcgcgctgc cgcccgccga gatcggcgag gacatcgcca tcggcgcgta cgtcgccgac
      301 cggttcggcc gcgaggtcgt cgaccggctc gtcgaaccgc tgctcggcgg cgtctacgcg
      361 ggcaacgcct accggatctc catgcgggcc gccgtgcccc agctcttcga ggccgcccgc
      421 acgcacccgc tgctcggcga cggcgtacgg gacatccagc gccgcgccgc ggagcagccc
      481 cgggcgggcg gccccgtgtt cgccggcatc gacggcggca tcgggcgcct cccgctcgcc
      541 gtggcgcggg cctgccgcga ggccggtgcg cggatcagca ccgggacccc ggtgcgcgag
      601 ttcttccgta ccaccgcggg ctggcgggtc gtcgccgacg gcggcgaggt catcgacgcc
      661 gacgccgtgg tcctcgccac cccggccggg cccgccgccc ggctgctgga cgcgctcgcc
      721 ccggccgccg ccgccgagct gcgcaccgtc gagtacgcct cgatggccct ggtcacgatg
      781 gccttccggc gctccgagct gcccgccgcg atcgccgggg gcggggccag cggattcctc
      841 gtaccgcccg tggacggccg tacgatcaag gcgtccacct tctccagcaa caagtgggcc
      901 tgggcgggcg ccgaccccga tctgttcctg ctgcgcacct cggtgggccg ccacggcgac
      961 gaggccgacc tcggccggga cgacgccgat ctggtggagg tctccctgcg ggacctcggc
     1021 gaggccgtcg gcctggcggc ccggccggtg gcctccaccg tcacccgctg ggacggcggg
     1081 ctgccccagt acccggtggg ccacctggcc cgtgtggacc ggatccgggc cgccgtcgcg
     1141 gccctgccgg gcctcgccgt gtgcggcgcc ctctacgacg gcgtgggcat ccccgcctgc
     1201 gtggcgagtg ccacccgggc cgccgacgtg gtgatggcca cgttgggcgc cccgttgggc
     1261 acccctggcg ccgaccactg atcagcacac gggacaatgg acacatgact gcaccagaga
     1321 agattcccaa cgcggggaag aaggcgaagg acctcaacga ggtcatccgc tacaccctgt
     1381 ggtccgtctt caagctgaag gacgtcctgc cgcaggaccg cgccggtttc gccgacgagg
     1441 tccaggagct gttcgaccag ctggccgcca aggacatcac cgtccgcggc acctatgacg
     1501 tctccggcct gcgcgccgac gcggacatca tgatctggtg gcacgccgag acctcggacg
     1561 agctgcagac cgcctacaac ctgttccgcc gcacccggct cggccgcgcg ctggagccgg
     1621 tgtggtcgaa catggccctg caccgcccgg ccgagttcaa caagtcgcac atcccggcct
     1681 tcctggccga cgaggtcgag cgcgactacg tcagcgtcta cccgttcgtg cgctcgtacg
     1741 actggtacct gctgcccgac gaggaccgtc gccggatgct cgccgaccac ggcaagatgg
     1801 cccgcggcta cccggacgtg cgcgccaaca ccgtcgcgtc cttctcgctg ggcgactacg
     1861 agtggatcct ggccttcgag gccgacgagc tgtaccgcat cgtcgacctg atgcgtcacc
     1921 tgcgcgcctc cgaggcccgt atgcacgtcc gcgaagaggt gcccttctac accgggcgcc
     1981 gcaagtccgt cgccgatctg gtggacgggc tcgcctgaga cctcccctgg ggggaaggac
     2041 cggaagaccg ctctcgtgac tgaggagggg cttccggtgg atcgggaggc cttgcccccg
     2101 aaacgacgca gccggagatc ccgcccggaa gttcagacga ctggcggcag cggcaccctg
     2161 cgggtgcccg ctgcccggtc gtccagcgac accggtgcgg gctccggatg cggcgcacac
     2221 gtgacactcc ccttgggggt gtcaccggtc aggatgtacc gctccacgtg ccggtccacg
     2281 cagtcgttgc gtccgccggc cactccgtgg gcgccggcac cggcctccgt gaccagcgcg
     2341 gccgctccgc ccagccgccg cctcagctcc agcgcacccg ggtacggggt cgcgccgtcc
     2401 cgctccgccg cgacgatcag cgtcggcggc aggggagtcg cccgcgagcc cacgtcgacc
     2461 gggcgctgcc gcaggcgctg cgccaccggc cagtacgcgc acggcaggtt caggaaggcg
     2521 ttcgcccagg tctccagggg agcccggcgg gccagttcgg tgtggtcgcg gtcccaggtc
     2581 tcccagtcgg cgggccaggg ggcgtcgttg cacagcaccg ccgtgtagag cgccgtggcg
     2641 ttctcggcct cggccgccgc gcgcgccgcc gccaccgggt ccgggtcggg cccggccagg
     2701 gcggccagcg gggccggatc gccgtccagg aaggcgctca gggccgccgc cagctccggc
     2761 cagacctcgt cgtagtacgc ggcccggagg gcggccgccc gcagttcgcc ggtgccggcc
     2821 accccgcccg ccggagtgcg ggccaccgcc gccctgacgc gctcgtgact ggcccgcacg
     2881 gcggccgggg tggcaccgag gcggtacgtg gcgtggtgcc gggccgccca ggcgcggaag
     2941 tcgtaccagc ggcgctcgaa ccccgccgcc tgggcgaggt tgtcgcggta ccagacgtgc
     3001 cgcgggtccg ggtcgaccgc ggagtcgaag accatccggc ggacgtggcc ggggtagagc
     3061 gtggcgtaca ccgccccgag gtaggtgccg taggaggcgc ccacgaaggt cagctcccgc
     3121 tcgccgagcg cggcgcgcag cacgtgcagg tcccgggcgt tgttgagggt cgtgtagtac
     3181 gggagggccg cgccgcccct tcgcgcgcag ccgcgggcgt aggcccgggc ggcggcgacg
     3241 cgctccctct tgtacgcggc cgaggggtcc gccgggacct gggtgggggc cttggggcgg
     3301 gccgccgggt cctggcagga cagcggggcg gagcggccga cgccgcgcgg ggcgtagccg
     3361 acgaggtcgt aggcggcggc gacgcggcgc caggcggggc ggtcggcgag cagcgggaag
     3421 tacatgccgg aggcgcccgg tccgccgggg ttgaagacga gggagccctg gcgggcggcg
     3481 cggccctcgc gggtggcgcc gacccggctg acggtgaggg agatctgggg tccgtcgggc
     3541 cgggcgtagt cgagcgggac gcgcagggcg gcgcaccgga cgggggcggc cagcccctcg
     3601 gcggcgggac agctccggaa gtcgagcgcc cggtgaccgg ccgcggcgcg gcgggcgacg
     3661 cgctcggccc cggcgttctc ggccgcgacc gcggtggcgg gggccgacgc cggggccggt
     3721 ggggcgaggg tgagggtcag ggcgaaggcg agggccgtcg cggcgacggc ggggcgtacg
     3781 agcggcatcc gcatggcggc cccttcatct cgtcggctgg tccgatgaga ttccggggcc
     3841 gcctcccggc caaggagcac cgccgggtgt cgtccgtgat tgccccgttg ccgtagcgcg
     3901 gacgggtcag gagctgctgg agcagctgga gctcgacgat ccgcagctcg aaccgccgcc
     3961 gctggagcag ctgctggagg aggagccgca gccggacccc gaagacccgc agccggagcc
     4021 cgacgaacca cagccggagc ccgacgaacc acagccggat ccggagctcc cgccgcagcc
     4081 ggagctcccg ccgcagccgg agctcccgcc gcagccgctg gagccgccgc cggacccgcc
     4141 gtcgacgctc gcgcaccaca cgaccggcag cgcgtcggcg gaggaagacc cccacgagga
     4201 acgggagccc ggggaaccgg cggagcggcg ctgccccgcc tgggccgccg ccagccgggt
     4261 gctgcgggcg gccggcacca gctgctcgcg caggtacggg tcacgcagcc cccgcagccc
     4321 gaacagggcc gtctgcacgt gcggggtctg gtccgtcaga tgggtggccc gcatcgcgcg
     4381 cagcgccgtc cgccccgccg gggtgatccg ccgcttggcc cgcgcggcgg cggcgacgcc
     4441 cacgacgagc ccgccgatca gcacggggaa caccttgacg aggaacggca gctggaaccc
     4501 gggctccagc gcgaacccga cgaaggtcag caccagcgag accggcagca gcgccccgca
     4561 cagcacggcc tgggccagac cccagcgccg ccaccgccgc gcggcgccgg gcgggctgat
     4621 cagcccgcgc tcggcgagcg cgtccccgat ctcctggatc gcggggtcgg tcatggccgc
     4681 gtaccgcacc tggtagagcc agcccgacgg cgcgttcgcg tacgcctgga ggacggcgcg
     4741 ctccgcgacg tccacggccc gcgccccggg ccgcacctgc acgatgcccg ggccgccggc
     4801 caccatccgc tggtcgccca gcagggagac gagcgcggcg tcgaccacgg cgccggggcc
     4861 gcccgccatg aaggcggcct cggacaggtc gagcgggggg tgcgcggggc cgcccggcgc
     4921 gggccgggag cggcgcaggc cgcgcagcag cagcaccgtg gaggtgatga cggcgatcca
     4981 gaccgcccag gcgaggaggt tcatgccgct ctccccacca gggcccgggc ccagcgcacg
     5041 atccggcggg ccgggcgcgt cccggcccgt tcctgccacc aggcggtgag ccggcgccgg
     5101 tcggcggggt cggccggcag ctcccggatc agcaggtgct cggcgaagtc cagcgcgtcg
     5161 cggcggtatc cgccggtcat ggggcggttg cgggcgtacg cgaggaacgc ctcgcggtac
     5221 gccttctcgc cgcccaggat gccggggagc tccggcgcca ccttggccac gaccccggcc
     5281 cgcttggcgg ccagcgaccg cgactgcacc cggatccgct gccggtcgaa gccctcgggc
     5341 accggcgtcc ccgccaccag cgcggacagc agcgcggcct gcgccagccc gacccgggtc
     5401 cgcgcggcgt ccggcacggg gtccgccgcg ggctccggcg cggaatgccg ggaaggggtg
     5461 cggagggagg cggaaggacg ggatcccgcg gggtccagca cgccccggat cgcctccagt
     5521 tccccggcga gctcgccctc cggcgggaag tcgtcgtccc gctccagcag cacgcccggc
     5581 gggtccaccc gccgccgcag ctgcgcgagt acgtccagca ccacgcccgg cacggggtgc
     5641 gcgtgggtgt cgtgccacac gccgtcccgc tcgaacccgc ccgcgacgtg cacgtacgcc
     5701 agcgcctcca gcgggatcgc gtccagtacg gcggccgggt cctcgccccg gttcacccgg
     5761 ttcgtgtgca ggttcgccac gtcgatcagc agccgcaccc cggtgcgctc gaccagctcc
     5821 gacaggaacc ggccctccgt catctcctcc ccgggccacg agatcagcgc ggcgatgttc
     5881 tccagcgcca gcggcaccgg cagcgcgtcc tgcgcgatcc gcacgttctc gcacagcacg
     5941 tccagcgcgt cccgggtccg cggcaccggc agcaggtgcc ccgcctccaa ggccggcgcg
     6001 ggcgtcgtgg tccgtacgaa cgcgatgtgc tcggtgacca gcggcgcccc cagcgccacc
     6061 gcccgctcgc cgagcgcggc cagcttctcc gggtcgggcc ggtgcgcgcc gccgaggccc
     6121 agcgagacgc cgtgcggtac gacccggaca ccgcgctcgc gcagccgcag cagcgaagcc
     6181 ggcagatggc ccgggcagat gttctcggcc acgacctcga cccagtccag ccccggcagc
     6241 cgctccacgg cgtccgcgat ctccggccgc cagccgatgc ccacccccag gtgtgccatg
     6301 ggcttcatgt cccctcgccc ccctcatcgg tccgtgtgcc catgtcatcg ccacggccgg
     6361 ggcgggccaa ccaccgccgc cggacgttca gaggaacatt tgaggttccg gaggtcccgc
     6421 cgtcacagcc gggcccgcag cgccggatgg tccgagacca ccgtggcgct gccgggcgcg
     6481 atctcagtga aaccggcgtc ccttacgacc ggcagaccgc tcccgctcag ccgcgcccac
     6541 cgctcgcgcg gggccgtccg tacggccagc cggaaaccgg aatcccgcca ggcggtccgc
     6601 tccgcgccgg tcagctccca ccacgccagc tgggccgcgt gcccggcctg ggccatcgcc
     6661 ttgccggcgg acatctccag atccgggttc agccacagca ccgcgatccc gtccggcgcc
     6721 ggagcgaccg gctccgggtc gtcgaggtcg gtccccgaga cctggagctt ggccagttcc
     6781 ttcggccacc cgtcgagcgg gaccggcggg aacacccgta cctccgccgc gtcaccgtgc
     6841 accgtgacac ccggcagggt ctcggccttg cgccactccg cgccgcgcgc ccgccggacc
     6901 accttgcgga tccgcgcgtc ctgccagtcg cggaccgccc cggcccattc gccctcgccg
     6961 agcgcccgct cgtcgctcag cagcaccagc accgcgcggg ccgccgtctc cagcgcgtcg
     7021 gtacgggccg ggggttcggc cttctcgatc cggacgacca gggggagcac gaactgcggt
     7081 gcctcgtcac gggcggaccg ctgcggccgc ggcgggtgct ccgcgcccgc ggcgggtacc
     7141 ggggtgctgt cgtcgtggtg cgtgtccggg ctgctcatcg cgctgttcat cccctgctca
     7201 tgccgctgtt catcctcgca aaggatgcca gcctccccct tcaggatgat gccgaggggg
     7261 ttcgggccgg gcgaggatcg aacccatgaa gagtgatctt ttcgcgtccg agcacctcgc
     7321 ccagccgtcc gccgccccgg gcatgaccct gcagaacgcc aagtccgtga agtacaccgt
     7381 caacggtgaa atgctggccc gtcagggctc gatggtcgcc ttccgcggca acctccagtt
     7441 cgagcgcaag ggccagggca taggcggcat gctcaagcgc gccgtcaccg gcgagggcct
     7501 ggcgctgatg tccgtacgcg ggcagggcga cgcgtggttc gcgcaccagg ccggcaactg
     7561 cttcatcatc gacttcgagc ccggcgacgc cctgaccatc aacggccgca acgtgctctg
     7621 cttcgacccg accctgtcct acgagataaa gatggtgaag ggcgccggca tgaccggcgg
     7681 gggcctcttc aacagcgtct tcaccggcac cggcaagctc gccgtggtct gcgacggcca
     7741 cccgatcatc atcccggtca ccgcccagaa cccggtctac gtggacacgg acgcggtcgt
     7801 cggctggagc gcccagctgg agacgggcct gcaccgctcg cagtccgtcg gctcgatgat
     7861 ccgcggcggc tcgggcgagg ccgtccagct gatgctgcgc ggcgagggct tcgtcatcgt
     7921 ccgcccgagc gagctgacgg aaaccgcggc cgcgcactga acaggacccc accgaagacg
     7981 acggccgtgc gacgggacgc catgcgactg gacgccgtgg gacgccgcta cggcgtgcgg
     8041 ggaccctggg tgctgcgcgg ggtctcgctg gagctgccgc ccggcgcgct cgtccgcgtc
     8101 gaaggacgca acggcggcgg caagtccacg ctgctgcggc tcgtggccgg catcgacacc
     8161 ccgaccgagg gaagcatcac cggccgcccg cgccgcaccg cttacgtacc cgagcgcttc
     8221 ccggccgtcc tgccgctcac cgcgcaggcc tacctcggcc acctgggccg ggtccacggg
     8281 ctgacgcgcg ccgaggccgc gcgccgggcg gcgcagtggc tggagcggtt cggcgcggcc
     8341 gggcacgccc gcaccccgct ggccgaactg tccaagggga ccagccagaa ggtggccgtc
     8401 gcccaggccc tgctcgccgg accggggctg ctcgtcctcg acgaggcgtg gaccgggctc
     8461 gacgtgcccg cgcgcgccga gctggaccgg gccgtggccg agcggaccgc cgccgggggc
     8521 acggtgctgt tcgtggacca cgacccggcg cggctcgccg gggcacccga ccgccactac
     8581 cgggtcgagg ggggcgcgtt ggaacccgtg cggccgggcg ccgggcccgg gccccgcgtg
     8641 cggatcgagg ccgccggacc gcccggcgcc gtcccgcccc ccggcctggc ggccgagccc
     8701 ctgcccgccg gcggattccg cctgaccgtg cccgccggcc ggtccgacgc ggtgctgcgc
     8761 gaactgctca ccgcacaccc tgcctggcac atccggaacg tgagtgacct gccgtgaccg
     8821 ctctgctgcg ctatcagggc gcgctgttgc tgcgctcgca gcgctggctc gcgccggtgc
     8881 tgctgtacgc cgccttcctc gccgtcgggt tccgccccgg ggatcccgta ccggactcgc
     8941 tgggctactc ggccgccgcg ctggtcccgg tgacggcctg gctggtgcgg atctgcctct
     9001 cggtcgaacc cgacgcggcg cgggactgcg cggccgccgc ggcgggcccg gtacgggtgc
     9061 acgcggcggc cctgctcacc gggctgggct ggggcgccgg cgcggggagc ctcggcgccg
     9121 tcgcggtgct gctgctcggc gcccgcgagg ggccgcggct cccggccgcc gccctggcgg
     9181 gggccgcgtc ggtgctggtg tgcgcgctga cgggcgcggc cgtgggcgcg ctgtgcgccc
     9241 ggccgctgct gagggccccc gggtacgcgg tcgcggcggc gggcctcggc agcctgctgg
     9301 ccctggtcac ggcgggctcg ccggcccggg tggcggtcag cgggctggtg gggggctcgg
     9361 tggcgctccc gctgggagcg ctggcgggcg cggcggcgct ggcctgcgcg gcgggcgcgg
     9421 ccgcctgcct ggccgccgcg cggcgggggt gagtacgctc ggggcatgga ccagtccgag
     9481 ccgaccgagt cgtactgccc cgcgccggcg gcgcccgccc gcgtcgtggc gggaccgccg
     9541 tacgcggact gcctggagtg cggcaagccc accgagtacg gggtccggac ccccggcgtg
     9601 gtgctgtgcc cggtctgcga gtggcaggaa gcccagcgca ccgcctgctc gggctagggc
     9661 ctgatcccaa agacaggccc taggtgccca tgagctcgga gaccttcacg aagcggtagc
     9721 cgcgcttgcg caactccggg acgatcgtgc ggatggcctc ctcggtgacg ggcgccgcgc
     9781 tgcgggtgca gtgcatgacc accagcgagc ccggcttcac cccggccagg acctgctcgg
     9841 ccaccgcgtc cgggtccttc gcgaaggcgt ccccactgac cacgtcccac tgcaccgccg
     9901 tgaccttggc cgtggacagg gcccgcagcg cctggtcgtc gtagcagccg ccggggaagc
     9961 ggaagtacgg gacggtgttg acggcgcccg ccttgcggaa ggcgtcgaag gcccggtcca
    10021 cgtcggcccg ggcggcggct ccgtcgagcg tggggagtcc gtagcagggg gacttgaagg
    10081 cgtggtggct gtacgagtgg ttggcgatct cgaagttcgg gtcggtgccg atggctttgg
    10141 cctggtccgg gtactcctcg gcccagcggc ccgtcatgaa gaccgtcgag ggcaccttga
    10201 gcgcccgcag cgtgtcgatc agctgggggt tgtcgaagtg ctccccgccc gccgcgcgcg
    10261 gtccctgatc ggaggtcatg tccgcgtcga aggtcagggc gacggtcttc tcggtcttct
    10321 gcctggcccg ctcgaagacc ggggtcaggc cgcccgggcc gggggccagc gtcggggcct
    10381 tgccgggggc gccggccctc gggagcgcgg aggccgctcc ggcggcgggg gcgggtgcgg
    10441 aggcccgggc cgacgcggag gcggaggccg agggggtggc ggacgcgccg agccgggcct
    10501 ggccggcgcc tccattcgag ggaccggcgc cgcccccgca gccggcgagg acggcgccga
    10561 gggcgccact gaggacggcc gcggccgccg ctttgcgcag ggaagaggtc acctacgcaa
    10621 aatatatgac tatgtggcaa tcagacgggc acggcactcc tccacgtcga acccggcctc
    10681 cgggtaccgc ggaccgatga gctccaggtg ctcccgcagc agagtggcga cggcccagtc
    10741 gcggtaccac ttgcggtccg cggggacgac gaaccacggc gcggcgtccg tggagcaccg
    10801 ctccagcgcc agctcgtagg cctcctggta cgcgggccac agcgcccgct cgtcgatgtc
    10861 ggacggggcg aacttccagt gcttggacgg gtcgtccagc cttcgcagga gccgctcgcg
    10921 ctgcttctcg tggctgatgt ggaggaagac tttcaccacg gtcaccccgt cccgcgcgag
    10981 ggactcctcg aaccggttga tctgcgcgta gcggtggttc acctcgctgc gggacaccag
    11041 ttcgcggacc cgggcgatca gtacgtcctc gtagtgcgag cggtcgaaga cgccgatctc
    11101 gcccggcttc gggagggcgc gggcgatccg ccacaggaag gggtgcgacc gctcctcgtc
    11161 cgtgggcgcc ttgaaggccc tgatccggca gcccgcgggg ttgagcgggc cggtcacgcg
    11221 cttgaccgcg ccgcccttgc cgctggtgtc catgccctgg aggacgagga gcacccggcg
    11281 gcggtcgccc gcggtgctc
//
