LOCUS       NZ_JANPRN010000029     12049 bp    DNA     linear   CON 24-FEB-2024
DEFINITION  Staphylococcus pseudintermedius strain WU3
            WU3_S1_L001_(paired)_contig_19, whole genome shotgun sequence.
ACCESSION   NZ_JANPRN010000029
VERSION     NZ_JANPRN010000029.1
KEYWORDS    WGS; RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 97380)
  AUTHORS   Abouelkhair,M.A.
  TITLE     Genome sequencing and comparative genomics of Staphylococcus
            pseudintermedius
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 97380)
  AUTHORS   Abouelkhair,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Biomedical and Diagnostic Science, The
            University of Tennessee, 2407 River Drive A222, Knoxville, TN 37996,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: QIAGEN CLC Genomics Workbench v. 22.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 01:14:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,582
            CDSs (total)                      :: 2,515
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 10 of 63
            Pseudo Genes (incomplete)         :: 51 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:27:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 22650
            Orig. end                         :: 34699
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JANPRN010000029.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12049
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12049
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12049
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7049](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7049)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(149..1930)
                     /gene="recQ"
                     /locus_tag="NVR04_RS04085"
                     /old_locus_tag="NVR04_04085"
     CDS             complement(149..1930)
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424488.1"
                     /locus_tag="NVR04_RS04085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_04085"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_015728744.1"
                     /transl_table=11
                     /translation="MEQTLSHYFGYKSFRPGQKEIIERVLNHHHTLGVLPTGGGKSLCY
                     QVPGLMFKGTTIVISPLISLMKDQVDQLTAMGISAAYLNSSLSTQAQKEIETQLVNGEL
                     KFLYIAPERFEQTYFEMLLRRVDISLVAFDEAHCISKWGHDFRPSYQAVIQRVLTMPQQ
                     FSIVALTATATTEVQQDIMERLMISRQHLVKTSIRRRNLKFQVNGTYQRQKFVLDYIQK
                     HSQKAGIVYCSTRKQVEALYEALEDADIPVSYYHAGMTAKQRDEAQNDFLYDRTRVVVA
                     TNAFGMGIDKSNVRYVIHYNMPGDLESYYQEAGRAGRDGLDSDCILLYSDRDIALHQYF
                     IGASKADDDYKERMGEKLTKMIQYTKTTKCLEATLVHYFEPNEKLEECHQCSNCIDKNK
                     TYNMTKEAKMIISGVARLRQQEGYQTVIQVLRGEVSDYIRHQGYENLSTYGLMKDYTTG
                     ELHHLIDELRFKGFLNEHDEILMCDASVEKLLSEDTQVFTRPFKRKSKETVHINTVEGV
                     DRTLFEKLVEVRREMSERLNMPPTNIFTDYTLEAFAKRKPVTKQEMIQIDGVGSYKLKH
                     YCPEFLEAIQEYKMHRS"
     gene            complement(1940..3817)
                     /locus_tag="NVR04_RS04090"
                     /old_locus_tag="NVR04_04090"
     CDS             complement(1940..3817)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166146.1"
                     /locus_tag="NVR04_RS04090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_04090"
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="WP_015728743.1"
                     /transl_table=11
                     /translation="MEAYKIERLNKSYADKVIFDDLQLSISNQERIGLVGINGTGKSSL
                     LKVIAGMDEDFDAVQSHPKQYRIRYAAQNHNLEPTLTVYEAVYSADSKELQVIKRYEEA
                     TAQYAEQQTDKAFDAMMAAQADMDAYSAWDYSAEIKTILSKLGIHDTTRRMSELSGGQQ
                     KRVVLARTLIEKPDLLLLDEPTNHLDFESIHWLIQFVKSYPKTVVFVTHDRYFLNEVAT
                     RIVELRNGKLDTYPGNYEAYIAMRAEREEIEVRQQTKQRALFKQELAWMRAGVKARTTK
                     QQARQDRFHDLKEQVQSHQVQGKGSLNLAYSRLGKQVFELENVTKRIDGRTLFENVSTL
                     IQKGVQIGIVGPNGVGKTTMLNILAGEDQHFEGVLKIGQTVKVAYFKQTDERLNRDVRV
                     IDFLREESELARQKDGTTVSITQLLERFLFPSVTHGKKVYKLSGGEQKRLYLLKLLVHQ
                     PNVLLLDEPTNDLDTETLTILEDYIQTFGGTVITVSHDRYFLDKVVDCYWYIHDGQIDM
                     ILGQFEDYLAYQKQLEQAATQVASVKQQPTLQPKKKKGLSYNEKREYDMLLVRIEETES
                     RLAEIDAEMIEANADYAKIKALNEEKETLEAQYDTDMTRWSELEEILEQ"
     gene            3929..4744
                     /locus_tag="NVR04_RS04095"
                     /old_locus_tag="NVR04_04095"
     CDS             3929..4744
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168536.1"
                     /locus_tag="NVR04_RS04095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_04095"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_015728742.1"
                     /transl_table=11
                     /translation="MLDYISNMPPFLQALLAGVITWLLTALGAATVFVFKSVNEKVLNS
                     MQGFAAGIMIAASFWSLLQPAIESSSGSTVPWLPAAIGFLLGGVFIRSLDYVIPHMHRN
                     ATDESQHQEGVETSLNKNTLLLLAITLHNIPEGLAIGVAFGGIATGNEHATLLGALGLA
                     IGIGIQNIPEGAALSLPIHASGQSKWKAFNYGQASALVEPVFATVGAAAVLVVTPILPY
                     ALAFAAGAMIFVVVEELIPDSQAGKNTDLATLSLMVGFTIMMILDVALG"
     gene            complement(5001..7049)
                     /locus_tag="NVR04_RS04100"
                     /old_locus_tag="NVR04_04100"
     CDS             complement(5001..7049)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="NVR04_RS04100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_04100"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_270866874.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 7.2e-26, bitscore:
                     83.2, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 2.1e-259, bitscore:
                     855.2, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFLRRKLRTTVNGTTAYGLVQVADKLGFETVPIKADISLFEIDD
                     VQYPFIVHVKKNNNHFHYYVVIRCDKKYIYIADPDPVVNLTKMTYREFKEEWTGVTILL
                     KPTSLYKPIKQDKVRLTSFIPLLLKEKRLIIKIIIISILVNMINILGSYFMKSIIDFYI
                     PQKLKTWVSMISMALIASYIIQQILSYLQKYFLILLVKTYSINILSPYIKHLFRLPITF
                     FETRRAGDILSRLNDANSITDALASTMLSLFLDVTIIVGISIFLYYQSTILFVLILIII
                     PIYAVIVFGFMNSFERKRIKSMEANATLSSYLMENITGMETIKALTCEKKCYKQIKYNF
                     SNYYEKTFLLNKLNIKQNVLKKVVQLILNVSILWVGANLVIDNQISIGTLLVFNSLVIY
                     FMNPIENIMNLQLKLQIAGVANNRLNEIFTVKSEFEKNKLDIKLNQNSSKLIFSNISYG
                     YGYEKEILTDINITIEHNSKVAFIGESGSGKTTLAKMMVSFFEPSKGKITLGGLDITNI
                     NKSVLRQQIIYIPQQTYIFNGTILENLLLGVVKEVTQKDIDSAIKMAELKSFIDSLPLG
                     LDSVLSSDFSALSGGQRQKIALARALLTNSSILIMDEATSNIDILTERKIINNLMALNK
                     TIIFISHNLNITQKVDKIFVLAKGKIIEEGSHEFLLKAKGYYSNLIN"
     gene            complement(6973..7164)
                     /locus_tag="NVR04_RS04105"
     CDS             complement(6973..7164)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /locus_tag="NVR04_RS04105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_197737724.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.2e-09, bitscore:
                     29.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFSKRFYRAQVDSQDCGAAALAMILEFYGSHYSFSLFFGFSEKK
                     VKNNSKWHYSIWFGSGSR"
     misc_feature    7343..7356
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: strong, score: 21.85"
                     /tool="antismash"
     misc_feature    7370..7385
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.05"
                     /tool="antismash"
     gene            complement(7539..7847)
                     /locus_tag="NVR04_RS04110"
                     /old_locus_tag="NVR04_04105"
     CDS             complement(7539..7847)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024424.4"
                     /locus_tag="NVR04_RS04110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_04105"
                     /product="DUF3884 family protein"
                     /protein_id="WP_063284913.1"
                     /transl_table=11
                     /translation="MIKISLKKILCQLSQKKLYEKTFQSIYIVDFSLLDRAPLFKDEFK
                     VIGTWYSYSGKRWICHTELSTEQFKKMITKNIDHKDLEKVKFYLDYLPFSITNEIPF"
     gene            complement(7922..8284)
                     /locus_tag="NVR04_RS04115"
                     /old_locus_tag="NVR04_04110"
     CDS             complement(7922..8284)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NVR04_RS04115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NVR04_04110"
                     /product="hypothetical protein"
                     /protein_id="WP_129923889.1"
                     /transl_table=11
                     /translation="MFKLLEESNHERFTYYMELLEKDFNHTQYFIFLKYPYYIKKLNID
                     ATNLLKSFLENYDLLNEGDVIFSKECTHSLLGASLFWGGYLKESKKVFEELEINSKDTV
                     CFYNKKFIYDCLSTLS"
     gene            complement(8341..9051)
                     /locus_tag="NVR04_RS04120"
                     /old_locus_tag="NVR04_04115"
     CDS             complement(8341..9051)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /locus_tag="NVR04_RS04120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_04115"
                     /product="glycosyltransferase"
                     /protein_id="WP_157978121.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 8.5e-21,
                     bitscore: 67.1, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKINKLEIISEYTTISVVYIVKNERDYIIKSLLSILDLADEII
                     IVDTGSEDNTLDLIKKMCDKKIKIFTFNWCDDFSKARNFANAKATCDWIMILDADEIVR
                     KNDNLKFYLTYLRIFDDFENTAFNFKVIRDEEVYKTSKLIRNTNTLTYKGRVHETFFSL
                     NNSVSYANLNVEVLGIRRGSQKKTNYYNKLLLKTIIDFPKEGRWYYLYSRYNIEDINYN
                     KMLKFCKKLYLKEM"
     misc_feature    10195..10208
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.13"
                     /tool="antismash"
ORIGIN
        1 attcattgta aaaataacac aatttgcatc taaaaaattt aaatcatcta ctatcgacac
       61 gtctcacttt tccattcttt accactcaat aagaaatcgt ttatttgtaa aaaaggagcc
      121 aggaattccc ccagctccat ctgatgtttt acgaacgatg cattttatat tcttgaatcg
      181 cttctaaaaa ttcaggacaa tagtgcttca gcttataact accaacacca tcaatctgaa
      241 tcatttcttg ctttgtgaca ggctttcttt tcgcaaaagc ttctaacgta tagtctgtaa
      301 aaatattcgt cggtggcatg tttaatcgtt cgctcatttc acgtctgact tcgacaagct
      361 tttcaaataa cgtgcggtcg accccttcta cagtgttaat atgcactgtt tctttcgact
      421 tacgcttgaa cggccttgta aacacttgtg tatcctcact caataacttt tctactgagg
      481 catcacacat gagtatttcg tcatgttcgt tgagaaagcc tttaaagcgg agttcatcga
      541 ttaaatgatg taactcgcca gtcgtatagt ctttcatcaa accatacgtc gaaagatttt
      601 catagccttg atgacgaatg tagtccgaaa cctcgccacg caaaacttga atgaccgttt
      661 gatatccttc ttgttgacgt aaacgtgcga caccgctaat gatcattttc gcttctttcg
      721 tcatattgta cgttttattt ttatcaatac aattactaca ttggtgacat tcttcaagtt
      781 tttcattcgg ctcaaaataa tggactaatg ttgcttctag acatttcgtc gttttcgtat
      841 attgaatcat tttcgtcaat ttttcgccca tacgttcctt gtaatcatcg tccgctttcg
      901 aggcaccgat aaaatactga tgtaaagcaa tatctcgatc actgtacaat aaaatacaat
      961 cactatctaa tccgtctcga cctgcacgtc ctgcttcttg ataatacgat tccaaatcac
     1021 ccggcatatt gtaatgaatg acataacgga cattcgattt atcaattccc ataccgaatg
     1081 cgttggtcgc aacgactaca cgagtacggt catataaaaa gtcattctgg gcttcatctc
     1141 tttgtttcgc tgtcattcct gcatgatagt aggatacagg gatatctgcg tcttctaaag
     1201 cttcatacaa ggcttctact tgtttacgcg ttgaacaata aacaatcccc gctttctgac
     1261 tatgtttttg aatataatcc aatacaaact tttgacgttg atacgtccca ttcacctgga
     1321 acttcaaatt acgtcgacga atactagttt tgactaagtg ttgtcgcgaa atcattaacc
     1381 gttccataat atcttgttgc acttcagtcg tcgctgttgc agtcaatgcg acaatcgaaa
     1441 actgttgcgg catagtcaat acgcgttgta tgactgcttg ataactcggc cgaaaatcat
     1501 gtccccattt tgaaatacag tgtgcttcgt caaatgcgac gagtgaaata tcgacacgtc
     1561 gcaacaacat ttcaaaataa gtttgctcaa aacgttccgg tgcgatgtat aaaaatttca
     1621 actcaccatt gaccaattgc gtttctatct ctttttgtgc ttgcgtactt aaactactat
     1681 tcaaataagc cgcagatatt cccattgcgg tcaattggtc cacttggtct ttcattaacg
     1741 aaattaaagg actaatgaca atggtcgttc ctttaaacat caatcccggt acttgataac
     1801 ataacgattt acccccacct gtgggtaaca cccctaacgt atgatgatga ttcaatacgc
     1861 gttcaataat ttctttttga ccaggacgaa aagatttgta tccaaaataa tgcgacaatg
     1921 tttgctccat gctatccctc tattgttcta atatttcttc tagttcactc caccgcgtca
     1981 tgtcggtatc atattgagct tcgagcgttt ctttttcttc atttaatgct ttgattttgg
     2041 cataatctgc gttcgcttcg atcatttcgg cgtcaatttc agccagacgc gactctgttt
     2101 cctcgatgcg cactaataac atgtcatatt ctcgtttttc gttgtatgat aatccttttt
     2161 tcttttttgg ttgtaatgtc ggttgttgtt tcacggacgc tacttgagtt gctgcttgtt
     2221 ccaattgttt ttggtatgct aaataatctt caaactgccc taaaatcatg tcaatttgac
     2281 cgtcgtgaat ataccaataa caatccacga ctttatctaa aaagtaacgg tcatggctca
     2341 cagtaatgac ggtaccacca aatgtttgaa tgtagtcttc taaaatcgtt aacgtttctg
     2401 tatccaaatc atttgttggt tcgtctaaca acaacacgtt cggctgatgc actaacaact
     2461 tcaataaata caaacgcttt tgttcgccac ccgagagttt atacactttc ttaccatgcg
     2521 tcacactggg gaataaaaag cgttccaaca attgcgtaat cgaaacggtc gtcccatctt
     2581 tttgacgcgc caactcactt tcttcacgca agaaatcaat gacacggaca tcacgattca
     2641 atcgttcatc cgtttgttta aaatatgcca cttttaccgt ttgacctatc tttaatacac
     2701 cttcaaaatg ctgatcttca cctgctaaaa tgttcagcat cgtcgttttg ccgacaccgt
     2761 ttgggcccac aataccgatt tgaacacctt tttgaatcaa tgtcgacacg ttttcaaata
     2821 atgtacggcc atcaatgcgt ttcgtcacat tttcaagttc aaagacttgt ttgcctaagc
     2881 gcgaataagc cagattcaat gaccccttcc cttgcacttg atgcgattgt acttgttctt
     2941 tcaagtcatg aaaacgatct tgccttgctt gttgcttcgt agttcgcgct ttgacaccgg
     3001 cacgcatcca cgctaactct tgtttaaaaa gtgcacgttg tttcgtttgt tgacgcactt
     3061 cgatttcttc ccgctctgct cgcattgcaa tatacgcttc atagttacct ggatatgtat
     3121 ctagcttgcc atttcgcaat tctacaatac gcgtcgccac ttcatttaaa aagtaacggt
     3181 catgcgtgac aaaaacgact gttttcggat aagactttac aaattgaatt aaccaatgaa
     3241 tcgattcgaa atctaaatgg ttcgtcggtt cgtctaataa taataaatct ggtttttcaa
     3301 tcaacgtacg cgctaacact acgcgctttt gttggccacc tgagagttca ctcattcggc
     3361 gtgtcgtatc atggatgcca agtttcgata aaatcgtttt aatctcagca ctataatccc
     3421 aagcactgta agcatccata tcagcttgtg ctgccatcat tgcatcaaac gctttgtcag
     3481 tctgttgttc tgcgtattgt gcagtagcct cttcataacg tttaatgact tgcaactctt
     3541 tggaatctgc actataaacc gcttcataaa ccgttaacgt tggctccaaa ttatgatttt
     3601 gtgccgcata acgaatacga tattgtttcg gatgcgattg aacggcatca aaatcttcat
     3661 ccatgcctgc gatgactttg agcaatgaac ttttccccgt tccattaata ccaactaatc
     3721 cgattcgctc ttgattggaa atggataact gtaaatcatc aaaaatgact ttatccgcat
     3781 aagatttgtt gaggcgttca attttatacg cttccacaac acatacaccc tttttcttat
     3841 agatttactc gcatttatct gttaacctta aatcagttct atatattata cacttttttt
     3901 ggaacaaatg atacaaggag ctatttctat gttggattat atttcaaaca tgccgccctt
     3961 tttacaagca ttacttgcag gtgtcatcac gtggttatta accgcattag gggctgcaac
     4021 cgtatttgta tttaaaagtg tcaacgaaaa agtgttgaat tcgatgcaag gctttgctgc
     4081 tggtattatg atcgccgcaa gtttttggtc attactccaa ccggccattg aaagcagttc
     4141 gggcagtact gtaccgtggc tccctgctgc aattggcttt ttactcggcg gggttttcat
     4201 ccgttcttta gactatgtta ttccacacat gcatcgaaac gcaactgatg aatcacaaca
     4261 tcaagaaggt gttgagacga gtctaaataa aaacacactt ttattacttg cgattacatt
     4321 gcataacatt cctgaagggc tcgcaatcgg ggttgccttt ggtggtattg cgacaggaaa
     4381 tgaacacgca actttgttag gggcactcgg acttgcaatt ggtattggga ttcaaaacat
     4441 tcctgaaggg gctgcattat cattaccgat acatgcttca ggacagtcca aatggaaagc
     4501 cttcaactat ggacaagctt cagcacttgt agaacctgtt tttgctacag tgggcgcagc
     4561 tgctgtattg gtcgttactc cgatactacc atacgcactc gcttttgctg caggggcgat
     4621 gatttttgtc gtcgttgaag aattaattcc tgattcacaa gccggtaaaa acacagatct
     4681 tgcgacacta agcttgatgg ttggctttac catcatgatg atattagatg ttgctttagg
     4741 gtaaattcaa aaaaccgttc aatgtgatga aacactgagc ggttttttga tgtgtacata
     4801 tatcgataca ttaaaaatac tctttaacaa ctcacaacaa agatttcaca ctgttgacta
     4861 tataaaatat caaccaatat accaaaatat aataattaac ttaagttata attgtaaaat
     4921 tcagaaaata aaaggtgtga aaaaacagta ccttcatttc gttcttcaca ttggttgtgt
     4981 taatacaaaa aataccatta ttaattaatt aagttagaat agtaaccttt tgcttttaat
     5041 aaaaattcat gtgagccctc ttctataatt tttccttttg ctaaaacaaa tattttatct
     5101 actttttgag ttatatttag attatgggag ataaatataa tagttttatt taacgccatc
     5161 aaattattaa taatttttct ctccgtgagt atatcaatat tactcgttgc ttcatccatt
     5221 atcaatatag aagaatttgt taatagtgct cttgctaaag ctatcttctg ccgctgacct
     5281 cctgacaaag cagaaaaatc agaacttaat acactatcaa gtccaagtgg taagctatca
     5341 ataaaggatt ttagttcagc cattttaata gcactatcta tgtccttttg cgtaacctct
     5401 ttaactaccc ctaataaaag attttcaagt atagttccat taaaaatata agtttgttgt
     5461 ggaatgtata taatctgctg tcgtaaaaca gatttattga tatttgttat gtccaatcca
     5521 cctagtgtta ttttaccctt tgaaggttca aaaaaactaa ccatcatttt cgccaaagtt
     5581 gttttacctg atccagactc tccaataaag gctactttac tattatgttc tattgttata
     5641 ttaatatctg taaggatttc tttttcgtac ccatatccat atgaaatatt tgaaaatata
     5701 agtttacttg agttttggtt caatttgata tccaatttat tcttttcaaa ttctgattta
     5761 actgtgaata tttcattcaa tcgattatta gccacacctg ctatttgtaa tttaagctgt
     5821 aaattcataa tattttctat tggattcata aagtaaatca ctaatgaatt aaaaacaagc
     5881 aaagttccaa ttgagatttg attatctata actaggttag cgcctaccca taaaatcgat
     5941 acatttaata ttaattgaac aacctttttc aaaacgtttt gctttatatt tagtttgttt
     6001 aacaagaatg ttttttcgta ataattcgaa aaattatatt ttatctgctt atagcatttc
     6061 ttttcacatg tcagtgcttt tatcgtctcc atacctgtta tattttccat caaataagaa
     6121 gacaaagtag catttgcctc catagatttt attcttttcc gttcaaaaga gttcataaaa
     6181 ccaaaaacaa ttacggcata aataggaatg ataattaata ttaatacaaa taaaatagta
     6241 ctttgataat acaggaagat agatattcca actattattg taacatctaa aaacaatgat
     6301 agcatggtac tagctaaagc gtcagtaatt gaatttgcat cattaagtct tgatagtatg
     6361 tctcctgctc ttctcgtttc aaaaaaagta ataggtaatc taaacaaatg tttaatgtat
     6421 ggtgaaagta tatttataga atatgtcttt actaataaaa tcaaaaaata tttctgcaaa
     6481 taactaagaa tttgttgtat aatgtaagaa gcaattaaag ccatggaaat catactaacc
     6541 catgttttta acttttgcgg aatataaaaa tcaataattg acttcataaa atatgaacct
     6601 aaaatattta tcatattgac taaaatggaa ataattatta tttttataat caaccttttt
     6661 tcttttaata ataaaggtat aaaagaagtt aaacgaactt tatcttgctt tataggttta
     6721 tataaagatg taggttttaa taagattgta actccagtcc actcttcttt aaattcccta
     6781 taagtcatct ttgttaaatt aacaacaggg tcagggtcag ctatatagat atacttttta
     6841 tcacatctaa ttacaacata atagtgaaaa tgattgttat tttttttaac atgaacgata
     6901 aaaggatact gtacatcatc aatttcaaac aaagatatat ctgctttaat aggaactgtc
     6961 tcaaaaccta acttatctgc tacctgaacc aaaccatatg ctgtagtgcc atttactgtt
     7021 gttcttaact ttcttctcag aaaatccaaa gaatagtgag aaagaatagt gagaaccgta
     7081 aaattctaat atcatagcga gcgctgccgc gccacaatct tgagaatcta cttgtgcacg
     7141 ataaaatcgt tttgaaaatt tcattcgaca cttctactcc ttcccaccac tttttagaac
     7201 aacatttaaa tattcaaaaa gcgggcggta attcccaccc acttaatatc atgtcaaaat
     7261 tcaatcctat tattaaaagc tttaatggtt attcatcttt acatattttt ttcatataat
     7321 cacatgcact ctctggatca gatgtaccga atctaaccaa tatactgtcg tttatacatg
     7381 taatctgagc ccatttacaa cgggacatcc caccacttaa gtctttcagc acctgatttg
     7441 atatcacttt aaatttatca atacttttta tatctatccc tcctaaatag aacaatttaa
     7501 taatactatt aaaaatcaat taaatgtatg atttattttc aaaacggaat ctcgttagtt
     7561 atagaaaatg ggagataatc caaataaaat ttgacttttt ctaaatcttt atggtctata
     7621 ttttttgtta tcattttctt gaattgctcg gttgataatt ccgtatggca tatccaacgc
     7681 ttaccactat agctatacca agttcctatt actttaaatt cgtctttaaa tagaggagcc
     7741 ctatctaaaa gactaaaatc aactatataa atactttgaa atgttttttc ataaagtttt
     7801 ttttgagata attgacataa aattttcttt aaagatattt taatcataga tttctcactc
     7861 ctttacacta cctgttatct taatgcggta gattcctgag ctataataat atcaatattt
     7921 attagcttaa agtcgataga caatcataaa taaatttttt attataaaaa caaactgtat
     7981 ctttactatt tatttctaat tcttcaaata cttttttact ttcctttaaa tatcctcccc
     8041 aaaataaaga ggctcctaat aacgaatgag tgcattcttt cgaaaaaatt acatctccct
     8101 catttaacaa atcataattc tctaagaaac ttttcaataa atttgtagca tctatattaa
     8161 gtttctttat atagtatgga tatttcaaaa aaataaagta ttgagtatga ttaaaatctt
     8221 tctctaacag ctccatatag taagtaaaac gttcatgatt ggattcttct aataacttaa
     8281 acatcaaaag aaaaataatt ctaacagagt aataattgtt gtttatttcg attccagata
     8341 tcacatctct tttaaatata attttttaca aaattttaac attttattat aatttatatc
     8401 ttctatatta tacctcgaat ataaataata ccaacgacct tcttttggga aatctataat
     8461 tgttttaaga agcaatttat tataataatt tgttttcttt tgacttcctc ttctaattcc
     8521 taaaacttct acatttaaat tagcataaga tacagaatta tttaaagaaa agaatgtttc
     8581 gtgtactcta cctttgtacg tcaaagtatt agtatttctt atcagcttac ttgttttata
     8641 tacttcttca tctctaatta ctttaaaatt aaaggccgta ttttcaaagt cgtcgaaaat
     8701 tcttaaatac gttaagtaaa actttaaatt atcatttttt ctaacaattt cgtcagcatc
     8761 taaaatcata atccagtcac aagttgcttt agcatttgca aagtttcttg cttttgaaaa
     8821 atcatcacac caattaaatg taaatatttt tattttttta tcacacattt ttttaattaa
     8881 atctaaagtg ttgtcttcag aaccagtgtc aacaataatg atttcatcgg ctaaatctaa
     8941 aatactcaaa aggcttttta taatataatc tctttcgttt ttaacaatat acactacaga
     9001 aattgtagta tattcactta taatctctaa tttatttatt ttttcaatca attctgcatc
     9061 gtttttaact cttttagact cccaatcttt tgattctata aatttatttt gttgtatgtt
     9121 tcacaagaaa tattaagaaa tgccttattt ttttctaacc aatcttcatc aaatataggg
     9181 tatatgtttt tcaaagttaa tcacaccccc tcaaaaaaaa aataataaaa caaaatattt
     9241 ttaaaagttt tgcaagactg agaggcgaat tattatgata aatgcgtaat tctactatca
     9301 cacttacaaa ttgctatgct ttaattataa ataattgtat tactttttaa atatagtgtc
     9361 catatttatt tatattagga agtataattt ctcacatata ctttaattac agaagtacat
     9421 gcagtgtaga tgacggttat aaaatgtagg aaaatggcga aatgaaaatg ctgctttttt
     9481 tatataaacg ccaagataaa tatggacaag tttattcttt ttatgaaagg aaatgtttta
     9541 atctataata ctttccaacg atagaggcga tcgagacggg ttctttgtca acgtcggttg
     9601 gtgagacaat aacggcactg agacattatg gtttcctaat ctctgcttct atttatagac
     9661 tgatatcgat taataatgat tgatccttcg aaaataaggg gtgaatgtgt ataaaaaatg
     9721 atgccattgt tttctggatt tcataatctt gcactcagtt tcgctgtatt gatataggat
     9781 tacccagatg gctgagacgc ctgaggggga acgtgcatac tgttgacagt cacctcttta
     9841 ctttaatggt gggaggcgtt tattgtagta gcgggctgac ttctcagtgt tttcactttt
     9901 gagaagtcta atcagccatg caaagcagta caacgaaatc ttgttgcata tgagatttct
     9961 gtactgctcc taaaatccaa tttggcttca atcaagccga attgagttga agggccggct
    10021 ctaggaaagc gaaaccatga tgacaattaa aaccacaaat cgtaatgcgg acaagtttaa
    10081 taaaaatatt acaacaatag catcaaaaat ttttaagacc cattccgttt tttatattca
    10141 cttacaagta tcttagaaat gattaagatt ccagaaattt ctataactat aagacacacg
    10201 tttgttttat tattaatcct ttcaatcggg ccatttgtta aatgaggatg atgcatagta
    10261 tttgaggttc agggcaaata tttgatgaat gtttgcaacg attaggtaaa atgtcgatga
    10321 tatcatgggt tacactatca cagtaaataa agctcattgg tccttcgata tctttcgttg
    10381 atttgaattc gtcaaaagct aagtgctcag gtaaagcatc atgtgcgtat gtctttacaa
    10441 attactcacc tcttttaaat ggcaatgaac agttgaaggt gatacacata gacttttagc
    10501 gacatctttt tcagatgtga atttagttag cttccgcgtc atcaagcgtt tgaccttatt
    10561 tgagacttcg gggttgaata tagggtgttt tagttataaa ggtttgttga caagctttaa
    10621 agtaaaaacg atgcttcttc aaatttaaat aaacaggtct ttcaaaatta aaaaacgggc
    10681 agtaaatctc tcaccaaccg ttaaaaatac acaaccaaat atatacaacc taaaaaacag
    10741 ggaaatgcac tgatttgtac acttccctat cataacattt tatttttgat ttgcctttgg
    10801 acctgtcgga tatttataat ctgctgggtt caatttgttg aaatcaggat ttttatagaa
    10861 tcggaataag tctccattca agactttatc actcatttct aagtcatgtt gtacctgctt
    10921 tttgtcctta tcaagatctg ctggtggtgt ttctaacggt tgattgtctc tgttactgta
    10981 ggctttaccg ttgacatatt tataatttct cgtcacgaaa tcaccattac ggaatggtac
    11041 aatttcttta tggtccttgg ataacatatc tgtaccaaac atgagataat ttttcgtatc
    11101 aatacctaac aagtgaagta atgttggcat cacatcaact tgacctgcat acgtgtcgtc
    11161 cactttaggc tcaacccctg gtgcttttaa ccagaaaccc gttgcattca agtcattaaa
    11221 cttcgctggt gtaatctctt cacctaataa ttgtgacatc gctttgttat gattttctga
    11281 gataccataa tggtcaccgt aaatcataat gactgaatca tcgtacagac cacgctcttt
    11341 caagtcatta atgaactgtt ctaatgactc atctaagtaa cgtgctgttt gaatgtaacc
    11401 atcgaccgtt gagttacctg tgtcaccttt tgcaatcgtt gcgtcttcat cacttaatgt
    11461 aaaaggataa tggttcgtca acgtaattaa atgtgaatag aacggttgtt tttctttaga
    11521 taagtaatcc gccgcctctt tgaaaaactc tttatctttt aatccaaggt tttctaagtt
    11581 ttctggagac atgtcaaaat atgttgcatc ataaaactta tcgataccaa aatgtctgta
    11641 tacttggtca cggttccaaa atgttttata gtcaccgtgc atgacatttg ttgtatagcc
    11701 ttgttgttga tgcataatag ctggtaaaga ttgaaaagta ttgtcacctt ttaatgaaaa
    11761 ggccgaacct tgaggtaaac catacatact gttgtccatt gtgaactcag cgtctgacgt
    11821 tttaccttgt ccagtttggt gatagaaatg tgggtaatat ctaaagtcct cattacctga
    11881 cgacaattta tttaaaaacg gcgtcacttc ctcaccatga atttttttat taattaggaa
    11941 tgtttggaag ctttctaaat ggattttaat gacattttta cctttagctt ttccatagta
    12001 ttccatattc ggtgtagctt gtttttgttt tgtataattc aatacttct
//
