LOCUS       NZ_JANPRN010000028     11044 bp    DNA     linear   CON 24-FEB-2024
DEFINITION  Staphylococcus pseudintermedius strain WU3
            WU3_S1_L001_(paired)_contig_30, whole genome shotgun sequence.
ACCESSION   NZ_JANPRN010000028
VERSION     NZ_JANPRN010000028.1
KEYWORDS    WGS; RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 133994)
  AUTHORS   Abouelkhair,M.A.
  TITLE     Genome sequencing and comparative genomics of Staphylococcus
            pseudintermedius
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 133994)
  AUTHORS   Abouelkhair,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Biomedical and Diagnostic Science, The
            University of Tennessee, 2407 River Drive A222, Knoxville, TN 37996,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: QIAGEN CLC Genomics Workbench v. 22.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Illumina MiniSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 01:14:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,582
            CDSs (total)                      :: 2,515
            Genes (coding)                    :: 2,452
            CDSs (with protein)               :: 2,452
            Genes (RNA)                       :: 67
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 10 of 63
            Pseudo Genes (incomplete)         :: 51 of 63
            Pseudo Genes (internal stop)      :: 14 of 63
            Pseudo Genes (multiple problems)  :: 10 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:27:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 40011
            Orig. end                         :: 51055
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JANPRN010000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11044
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6044](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6044)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(190..1404)
                     /locus_tag="NVR04_RS03550"
                     /old_locus_tag="NVR04_03550"
     CDS             complement(190..1404)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR04085"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613469.1"
                     /locus_tag="NVR04_RS03550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_03550"
                     /product="radical SAM/SPASM ryptide class RiPP maturase"
                     /protein_id="WP_110168739.1"
                     /sec_met_domain="Fer4_12 (E-value: 2.5e-13, bitscore: 43.5,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 6.9e-09, bitscore: 28.4,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 6.5e-09, bitscore:
                     29.1, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFDYRLREWLDTPSSNKNQFSSDTLYNELLLSESKKKIQISLAI
                     TYSCNLRCSYCFQQNYDHLVRKPITIEALEKILDKISLILYQNPEVDVSIGLFGGEPLL
                     PQNEKIIDRVFQYCVENKLKIDITTNGSFLPYSAKKIVIHRSIISVIAITINTLPDKYN
                     ETIKITKVANNVEELLAVTDLLLHYHIVVDVGTNFDRNNLSELLPIYHYFFDKGYFSKW
                     NFHWNIGRVDDRLFDTGYDEYIVSETDILLQLMKIPKPVPSNLHAGFIQTCKNLTDKLN
                     LSFNESQIKGTYHYCWNVSPYDRVFYIDNELNLFRCTVTVGRPQYILGNLKDIDLMNYQ
                     HETKSFLDYKDCQICHIGGFCSGGCKLSADIDFVKQCEMEKKEFEKFVEAILIPQVREK
                     LNRND"
     gene            complement(1656..>1886)
                     /locus_tag="NVR04_RS03555"
                     /old_locus_tag="NVR04_03555"
                     /pseudo=""
     CDS             complement(1656..>1886)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613462.1"
                     /locus_tag="NVR04_RS03555"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_03555"
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /pseudo=""
                     /transl_table=11
                     /translation="DVVTVDPPRKGCDATFIETLNALAPKRIVYVSCNPSTQLRDIEML
                     SAQYELKEMTPVDMFPHTTHVETVALLVRKY"
     misc_feature    2025..2039
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.13"
                     /tool="antismash"
     misc_feature    complement(2213..2227)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 24.36"
                     /tool="antismash"
     gene            complement(2419..3786)
                     /locus_tag="NVR04_RS03560"
                     /old_locus_tag="NVR04_03560"
     CDS             complement(2419..3786)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="NVR04_RS03560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_03560"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_270866869.1"
                     /transl_table=11
                     /translation="MSILTLKNIRCEMDGKLLFEADGLTIEEGDVIGLIGKNGAGKTCL
                     LKILAGELSDYAGYISGNRLTYFSELCITEDMTQSGGELSISSFLNALRQSVPLYLLDE
                     PTTYLDQHHLKKVVRTIRRSPSSFCIASHDRAFLDAVATKIWLLEQGKITEYNGSFSDY
                     VLARSMQLSQYEQELKQYQKEKKKIKQSLRMMKEKQDQKKGKPKKMSTSEYRIAGIKTK
                     YGVKQKKLQKRITRQQKKLEQLKQPYQVEDKYDISFLSQLHCLPKRKIIIPSHEAKIGR
                     KSLWHTPTIHLNSGDKLGIIGRNGAGKTTYLNYLISVLPSTYKICYFRQNHFQYLDPKM
                     TVYEAIVEATNGYLSEHHIRTLLALLDFKRDKVFRLIGDLSRGEHVKVSLLSLLVSESD
                     ILILDEMTNFLDIKAIEAVEKVLATYSGILIFVSHDQYFVQGLATSILDIDTYKIM"
     gene            complement(3800..4990)
                     /locus_tag="NVR04_RS03565"
                     /old_locus_tag="NVR04_03565"
     CDS             complement(3800..4990)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="NVR04_RS03565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_03565"
                     /product="MFS transporter"
                     /protein_id="WP_014613465.1"
                     /transl_table=11
                     /translation="MKTKRIIASDFLTTMMSSALTIYIYWFAYQYFSSQLIISIIGFGQ
                     LSGVFLSSLGGGISDSMNKLFFIKTLNFFKVGLLFMVLILENVVNMEILLPLFMFASTV
                     IGGLQSPTLESLVPFLASSDEALFRINAVVSSSTQVASIMAIVLSAVYISFLSFVTIVF
                     VTLFITAIATILLVGLTVETPLKSTSVVNNMKHGASYIFKTPYIRNLIPVALVMNFSFW
                     SIFLLLPKIANDSFSFLKTSYSGMELSFALGAGLGGILFAKFFYDVKDKYRLFKRSLFS
                     QSYVLLLLGIVLLIPNSVLAYTGMLLCWFLYALINTIFSILYFGKIQLNVPREMIGSVF
                     GAILTLFSLVNPIAAIMSGVLVSHFKIPLLIILLSSLMLLAAWSIRFLADVETVFD"
     gene            complement(5001..6044)
                     /locus_tag="NVR04_RS03570"
                     /old_locus_tag="NVR04_03570"
     CDS             complement(5001..6044)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="NVR04_RS03570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_03570"
                     /product="YcaO-like family protein"
                     /protein_id="WP_014613464.1"
                     /sec_met_domain="YcaO (E-value: 1.3e-23, bitscore: 77.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLENVSEHGVSGFYTYHFTEGNSFRPCATRIIEKEAIKATKDEFM
                     ELMLISSKSFPFVFFGGKSLGNFKLPIHLHPFMWQKRTIQDVNWYNEKNVEYIPFYNYK
                     DGSLKYIPKELSVYYEKAKGIGLSFSPQEGYSYDRDIVLAKVKAVMELIEKDIVTLWWH
                     RETSSRIIVIDDDTDENIKIIRNQLLNQGIDITLLDIENDLGVYVVVCILKQDDFPSIT
                     YGSAAHFEIKGAVKHAIFEAISCIVGLRYEFIHFKKIRTSFTYPDFISDSPLTNLSDYK
                     EILNLKTAINKYDIYYSYISADEGYLVKAYCYELQPTLYSATVPLTKRFFRVNKKEVRI
                     KEDFPFI"
     gene            complement(6097..6846)
                     /locus_tag="NVR04_RS03575"
                     /old_locus_tag="NVR04_03575"
     CDS             complement(6097..6846)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NVR04_RS03575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="NVR04_03575"
                     /product="hypothetical protein"
                     /protein_id="WP_037542633.1"
                     /transl_table=11
                     /translation="MGGINCGLSNNRVVKNDMYRLEYALYTKYVFKCFENFCNANTWVE
                     EFTLLEIDDNDNALVAYKKALFAIQEAITNLQSAKNALINSYNEIAPLFKFSQLKNGSI
                     NQYKKYDHSFAKGVFEKAGVVTNETKVWESVIHSLQNGGEVDYLNDQQNKLIVFENTLQ
                     NLFEEYQKLEKYIRQGVSHVAIRDIEVDITPLTAMALTKISELMSSLTYLCLVEYSAHT
                     SITGKTIDVLDLLPKTNKNSIQHLKAN"
     misc_feature    6891..6906
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.45"
                     /tool="antismash"
     misc_feature    complement(6917..6931)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 26.38"
                     /tool="antismash"
     misc_feature    complement(6993..7011)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.21"
                     /tool="antismash"
     gene            complement(7161..8525)
                     /gene="rlmD"
                     /locus_tag="NVR04_RS03580"
                     /old_locus_tag="NVR04_03580"
     CDS             complement(7161..8525)
                     /EC_number="2.1.1.190"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014613462.1"
                     /locus_tag="NVR04_RS03580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_03580"
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="WP_101485342.1"
                     /transl_table=11
                     /translation="MVLVKKNEVYEGKVVDLTHEGHGVIKQDRYPIFIPQTLIDEEVQY
                     KVIKVKKNFAIGKLMNVKTASPDRVTPPCDYYQQCGGCQLQHLSYRAQLEMKRKQVINL
                     FHHKGKMTEVPIHDTIGMDDPWRYRNKSQIPVGTNRNGQIQMGFYRQRSHDIIDMDHCL
                     IQNDDQNDMMNVIKSLLEKYHIPAYNEHIHQGLVRHVVIRKGYYTNEVMVIFVLNGKKL
                     PHHNDIVTALTSQFQQIVSIKLNFQTNKTNVIMGHTSKTIFGKDIIEDTLDDLTFEIGD
                     LSFYQINVTQTQKLYRQALQYANLTGNEVVLDAYCGIGTISLFMAPHAQHVYGVEVVPE
                     AIEDAKRNARYNQLNNTTFVAGAAEDVILKWQQDGIQPDVVTVDPPRKGCDATFIETLN
                     ALAPKRIVYVSCNPSTQLRDIEMLSTQYELKEMTPVDMFPHTTHVETVALLVRKY"
     gene            complement(8697..9629)
                     /locus_tag="NVR04_RS03585"
                     /old_locus_tag="NVR04_03585"
     CDS             complement(8697..9629)
                     /EC_number="2.7.1.107"
                     /GO_function="GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166450.1"
                     /locus_tag="NVR04_RS03585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NVR04_03585"
                     /product="diacylglycerol kinase"
                     /protein_id="WP_014613461.1"
                     /transl_table=11
                     /translation="MRKRARIIYNPTSGKEVFKRALPDVLIKMEQAGYETSAYATQKVG
                     DATEEAARAIEAQYDLLIVAGGDGTLNEVVNGIAEKPNRPKLGLIPMGTVNDFGRALHL
                     PTDIFEAVDVILDGKTVQVDIGKMNSRYFINLAGGGKITEVSYEAPSKLKSIVGPFAYY
                     IKGFEMLPQMHAVDVRIEFDSQVFEGEIMLFLLGLTNSMAGFEKLVPDAKLDDGMFTLL
                     IVEKANIAELGHIMTLASRGEHIKHPKVHYHKAQSVNISSFSDMPLNVDGEYGGQLPAN
                     FLNLVRHIEVFSPAQEDNALLIDEPTQSE"
     misc_feature    complement(10220..10229)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     misc_feature    10711..10725
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.65"
                     /tool="antismash"
     misc_feature    complement(10713..10724)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.25"
                     /tool="antismash"
ORIGIN
        1 aacatttttt tggtaactaa agggactaaa aaacttgcaa taaaagaaga taaacctatc
       61 agaataggca ccattgatgt cgcaatgact gactttgtga tggtgaatac atttgcaatg
      121 actacaactc taagaaacac atcagctaaa ttactaactg cttgagaaat caataattgt
      181 ttgattgcct tagtcatttc tgttcagttt ctcccttact tgtggaatta aaatggcctc
      241 cacaaacttt tcaaattcct ttttttccat ctcacattgt ttaacaaaat caatgtcagc
      301 agataactta cacccaccag aacaaaaacc gcctatatga caaatctgac aatctttata
      361 atctaagaag ctttttgttt catgttggta gttcattaaa tcaatatctt ttaagtttcc
      421 taaaatatat tgagggcgcc ctactgttac agtacatcta aacaaattaa gttcattgtc
      481 aatatagaaa actctatcat agggagacac gttccagcag taatgatatg tgcctttaat
      541 ttgagattca ttaaaagaca agttcaattt atcagttaag ttcttgcacg tttgaataaa
      601 gcctgcatga aggtttgatg gtactggttt aggtattttc attaattgta gaagtatatc
      661 agtctcagaa acaatatatt catcataacc agtatcaaaa aggcgatcgt caacccttcc
      721 gatattccaa tgaaaattcc actttgaaaa ataccctttg tcgaaaaaat agtgatagat
      781 tggcaacagt tcacttaaat tatttctatc aaaatttgtc cccacatcta caactatatg
      841 atagtgtaat aataaatcag taacagctaa taattcctca acattgtttg caactttcgt
      901 tattttaatt gtctcattat atttgtccgg taacgtatta atagtaattg ctataacaga
      961 tataatactt ctatgtatca ctatcttttt ggcagaataa ggcaaaaagc ttccgttagt
     1021 agtaatgtct atcttcaatt tgttttcaac acaatattga aaaactctat caataatttt
     1081 ttcattttgt ggcaatagtg gttccccacc aaataaccca atagaaacat caacttctgg
     1141 attttgataa aggattaatg aaatcttatc taaaattttt tcaagtgctt ctatagttat
     1201 tggttttcta acgagatgat catagttctg ctgaaaacaa taactacatc ttaaattaca
     1261 agaataagta attgctaaag atatttgtat tttctttttt gactctgata acaaaagttc
     1321 attataaagt gtatcagaag aaaattgatt tttattagaa gaaggtgtat ccaaccattc
     1381 acgtaatcta taatcaaatt ccatttctta atcttatatc tttaatatag cgtatgaata
     1441 cagaatatgt aagtgtgtat tttgaatatt cagaacaatg attttggtat atttttagaa
     1501 taacttcaaa acttcattac tcgccattct ctagagatta acactgtcaa atcagttcca
     1561 gtatcaacgc tcctaaaata gcaccaaaac aaaaaagggt tctaagtcac attctcaaaa
     1621 atgttctcaa aaaacccttg agtgattaat ctatatcaat attttcttac taataacgca
     1681 actgtctcga catgtgtcgt atgcggaaac atatcgacag gtgtcatttc ttttagttca
     1741 tattgcgcgc ttagcatttc aatatctcgt aattgtgtac ttggattgca tgacacatag
     1801 acgatacgtt ttggggctaa cgcgtttaaa gtttcaataa atgtcgcgtc acaccctttg
     1861 cgcggtgggt caactgtgac cacatcattc tacgctgatt cgaaaaatca actactgtag
     1921 cattcttgca gtcgtgagtc tcccctttat caaaacgtga gagttggaat gatgatatcc
     1981 ctcttttaga aatttcctta aacgtaaccc ctttgtaatt tcttattatt ctgaaattta
     2041 tagcgtgatt tttcatatgt tacctacgtt tcacacagaa attacgaatt atcattgcat
     2101 ggtttcattt ttatttttga tgacattggt ttaccgtcca aaaataaaat aactttcttt
     2161 aataatggat attgaacact cacctttaac ttgatcacgg aggctacaca acttttaaaa
     2221 atgagaacat cacatctctg cgtcaataat atagcattac agctagtagt ctgtacctta
     2281 aaacttttat tgtttttgtt aaactgtcat caaaacaggg tacctaatca aacggacgtc
     2341 ctttttatac tatcactatt tgatagatgc ttgatggagg gagattcttt cactccaaaa
     2401 tatttgtaat tgtccatcct acattatctt ataggtatca atatctagaa tcgaagtagc
     2461 taaaccttgt acaaaatact gatcatgact cacaaatatc agtatgcctg aatatgtagc
     2521 aagcaccttt tccactgctt cgatagcctt tatatctaaa aagttggtca tttcatctaa
     2581 aatcaaaata tcagactcag aaactaagag agataataat gaaaccttca cgtgctcccc
     2641 tcggcttaaa tctcctatta atcgaaatac cttgtctctt ttaaaatcta ataacgccaa
     2701 caaagttcta atgtgatgtt cgcttaaata cccattcgta gcttcgacaa tcgcttcata
     2761 tactgtcatc tttgggtcta agtactgaaa atgattttgg cgaaaataac aaattttata
     2821 tgtagaaggt aaaactgaaa ttaagtaatt caaataagtt gttttgcctg caccatttct
     2881 tccaataatt cctaatttat caccactatt taaatggata gttggggtat gccacaagct
     2941 ttttcgcccg attttcgctt cgtgactagg aatgataatt tttcttttgg gtaaacaatg
     3001 aagttgtgat aaaaatgaaa tatcatattt gtcctcaact tgatatggtt gttttaactg
     3061 ttctaacttt ttttgttgtc gtgtaatacg cttctgtaac tttttttgtt tcacaccgta
     3121 cttagttttg atacccgcaa tacgatattc tgaagtactc atcttctttg gttttccctt
     3181 tttctgatct tgcttttctt tcatcattcg aagtgattgt tttattttct ttttttcctt
     3241 ctgatattgt ttcaactctt gctcatattg tgataactgc attgaacgtg ctaacacata
     3301 atctgaaaaa gaaccattat attctgttat ttttccttgc tctaacaacc atattttagt
     3361 cgcaactgca tctaaaaaag cccgatcatg gctcgctata caaaaagaag atggggagcg
     3421 tctaatcgtt cttactactt tcttcaaatg atgttgatct aaatacgttg taggttcgtc
     3481 caacaaatag aggggcaccg actgtcttaa tgcgttgaga aaacttgaaa tgcttagttc
     3541 tccaccactt tgagtcatat cctccgttat acagagttca ctaaaataag tcagacgatt
     3601 gcccgatata tagccagcat aatcgcttaa ctctcctgct aatattttta acaaacacgt
     3661 ctttccagca ccgttctttc cgataaggcc aatgacatcg ccttcctcaa tcgttaaacc
     3721 atccgcttca aataataatt tcccatccat ctcacacctg atgtttttaa gtgttaatat
     3781 agacatatcc ctcccccctt taatcaaata ccgtttcaac atctgctagg aaacgaatac
     3841 tccaggcagc taacaacatg agtgaagaca acaatataat taacagaggt attttaaaat
     3901 gcgaaacgag aactcccgac atgatggctg cgatgggatt gactaatgaa aagagcgtta
     3961 aaattgctcc aaaaacactg ccaatcattt ctctagggac attcaattga atctttccaa
     4021 aatagagaat agagaaaatc gtgttgatca aagcatacaa aaaccagcac aacagcatcc
     4081 cggtataggc aagaacactg ttaggaataa gtaaaacgat tcctaataac aacaaaacat
     4141 aactctgtga aaacaaactc ctcttaaata aacgatattt atctttaaca tcatagaaga
     4201 atttcgcaaa taagatcccg cctaagcccg ctcctaaagc aaaggagagc tccatgccac
     4261 tataagaggt ttttaaaaat gaaaaactat cattagcgat tttaggtaag agcaagaaaa
     4321 tactccaaaa cgagaaattc atgaccaatg cgacaggaat aaggttacga atataaggcg
     4381 ttttaaagat ataactcgcc ccatgcttca tattattaac aactgaagta gacttcaatg
     4441 gcgtttccac cgtcagtcct acaagtaaaa tcgttgcgat ggcagtgata aaaagggtca
     4501 caaaaacaat cgttacaaaa gacagaaatg aaatatagac tgcagataaa acaatcgcca
     4561 taatagatgc aacctgtgtt gaactcgaaa ccacagcgtt aatacgaaaa agcgcctcat
     4621 cgctactggc taaaaatgga accaatgatt ctaacgtagg tgactgtaaa cctcctatca
     4681 ctgttgaagc aaacataaaa agtggcaaca aaatttccat gttcacaacg ttctctaata
     4741 tcaacaccat aaaaagtaat ccgactttaa agaagttcag agttttgatg aaaaacagct
     4801 tgttcatact gtctgagatg ccaccaccaa gacttgagag aaacacacca cttaattgcc
     4861 caaaaccgat gattgatatg atgagttgac tagagaagta ttgataagca aaccaatata
     4921 tataaatcgt taaggctgaa gacatcatcg ttgttaaaaa atctgacgca ataattcttt
     4981 tagttttcat aactcacctt ctatatgaac ggaaaatctt ctttaatcct gacttccttc
     5041 ttgtttactc taaagaaacg tttagttaaa ggaaccgttg cactatacaa ggtgggttgt
     5101 aattcataac agtacgcttt cactaagtat ccttcatcag cagaaatata agaataatat
     5161 atatcatatt tattaatggc tgtttttaag ttcaatatct ctttataatc ggataaatta
     5221 gttaatggac tatcactaat aaaatcagga tacgtaaaac tagtacggat ttttttaaaa
     5281 tgaatgaact catacctcaa acccacaata catgaaatcg cttcaaaaat tgcatgttta
     5341 actgcacctt taatttcaaa atgcgctgca ctaccataag tgattgatgg aaagtcatct
     5401 tgcttgagga tacacacaac cacataaaca cctaaatcat tttcaatatc taataacgtg
     5461 atatctatac cttgatttaa taattgattt ctaataattt ttatattctc atcagtatca
     5521 tcatcaatca caataattct acttgatgtt tctctatgcc accacaaagt aacaatatct
     5581 ttttctatta attccatgac cgccttgact ttagcaagta caatatctcg atcataggaa
     5641 taaccctctt gaggactaaa agatagccca atccctttcg ctttttcata atagacgctt
     5701 aattctttcg gtatatactt caaagatccg tctttgtagt tataaaaagg gatatactca
     5761 acattctttt cattatacca attcacatct tggatagttc gtttttgcca cataaacgga
     5821 tgaagatgaa tgggtagttt aaaattacct aaggatttcc ctccgaaaaa tacaaacggg
     5881 aaagatttac ttgaaataag cattaactcc ataaactcat ctttagtagc ttttatagct
     5941 tccttttcaa taattcttgt agcacatggt ctaaatgagt ttccctctgt aaagtgatat
     6001 gtgtaaaatc ctgaaacccc atgttcagaa acattctcta acatattaat ccccctacat
     6061 aaaaaaactg taccgtatag acacagtata taaaaatcaa tttgctttta gatgctgtat
     6121 actatttttg tttgtttttg gtagtaaatc caatacatca atcgttttgc cagtgatact
     6181 tgtatgggct gaatattcaa ctaaacataa gtatgttaat gaactcatca attcactgat
     6241 tttagtcaag gccattgcag ttaacggcgt aatatcaact tcaatatctc ttattgcaac
     6301 atgtgataca ccttgtcgta tatacttttc taatttttga tattcctcaa atagattttg
     6361 caaagtattt tcaaaaacaa tcaacttatt ttgttgatca tttaaataat ctacttctcc
     6421 tccattttgc agagaatgaa taactgactc ccaaaccttt gtctcgttcg tcacaactcc
     6481 tgccttttca aatacccctt tagcaaagga atggtcatac tttttgtatt ggtttattga
     6541 accatttttt aattgactaa atttaaataa aggcgcaatc tcattgtaac tattgattag
     6601 tgcattttta gcactctgta aattagtaat cgcctcttga atggcaaata atgctttttt
     6661 ataagcaact agtgcgttat cattatcatc aatttccaaa agtgtaaact cttcaaccca
     6721 tgtatttgca ttacaaaaat tttcaaaaca tttaaacaca tattttgtgt ataaagcata
     6781 ttctaatcga tacatatcat tctttacgac tctattattg gaaagtccac aattaatacc
     6841 acccataatg gcacctcctc ttaatattcc atcatgaatg tagcatatat atacacaaat
     6901 gtaaactatt tttccaaatt ttctgaatat taattctaga aagatattaa taaacacaaa
     6961 atgtctaatc tcaatatttt tacaatttca caactgtgtt tctaaacaat actttaaaaa
     7021 agctaatgtc tattaacgac aaacctattt gtcaaatcag ttccagtatc aacgctccta
     7081 aaatagcacc aaaacaaaaa agggttctaa gtcacattct caaaaatgtt ctcaaaaaac
     7141 ccttgagtga ttaatctata tcaatatttt cttactaata acgcaactgt ctcgacatgt
     7201 gtcgtatgcg gaaacatatc gacaggtgtc atttctttta gttcatattg cgtgcttagc
     7261 atttcaatat ctcgtaattg tgtacttgga ttgcatgaca catagacgat acgttttggg
     7321 gctaacgcgt ttaaagtttc aataaatgtc gcgtcacacc ctttgcgcgg tgggtcaact
     7381 gtcaccacat caggctgaat cccgtcttgt tgccatttta aaatcacgtc ttcagcagca
     7441 ccagcgacaa aagttgtatt gttcaattga ttgtagcgcg catttctttt cgcatcttct
     7501 atcgcttcag ggacaacttc tacaccatat acatgttggg cgtggggtgc cataaataaa
     7561 ctgatcgtcc caataccaca atacgcatct aatacgactt cattacccgt taaattcgca
     7621 tactgtaacg cttgacgata caacttttgc gtttgtgtca cattgatttg ataaaaggac
     7681 aaatcgccaa tttcgaaagt caaatcatct aatgtatcct caataatgtc tttaccgaaa
     7741 atcgttttgg acgtatgccc cataatgaca ttcgttttgt tcgtttggaa attcaacttg
     7801 atactcacaa tttgctgaaa ctgtgatgtt agagcggtca caatatcgtt atgatgtggt
     7861 aactttttac cgttgagcac aaaaataacc atcacttcat tcgtataata gcctttacga
     7921 atcacgacat gacgtacgag accttgatgt atgtgttcgt tataagctgg aatgtgatat
     7981 ttttccagta aacttttaat cacattcatc atgtcgtttt gatcatcatt ttgtatgaga
     8041 caatggtcca tatcgataat atcatggctg cgttgacgat aaaagcccat ttggatctga
     8101 ccattgcgat tcgtcccaac cggaatttga gacttgtttc gataacgcca aggatcatcc
     8161 attccaatcg tgtcatgaat cggcacctct gtcatttttc ctttatgatg aaacaaatta
     8221 atcacttgtt tacgcttcat ctctaattgc gcacgatacg acaaatgttg aagttggcat
     8281 cccccacact gttggtaata atcacatggc ggtgtaacac gatctggact tgccgttttc
     8341 acattcatca gcttaccaat cgcgaaattc tttttcactt taatcacttt atattgcact
     8401 tcttcatcaa ttaacgtttg agggataaat atcggatagc gatcttgttt aatcacacca
     8461 tgcccttcat gtgtcaaatc aaccactttt ccctcataca cttcattttt ctttactaaa
     8521 accatcaatt tcatctcact tttttataaa cagaatctac ttcaaaggga aaggagcagg
     8581 accaaaaact taacatctga gtcaaatttc atcgtcccgc tcccacgaga gtaatgtcat
     8641 tacatcatgc ggttttgcgt acactaccta tttattttca gcacacctct ggatcgttac
     8701 tcagattgcg taggctcatc aattaacaac gcattatctt cttgcgccgg agaaaaaact
     8761 tcaatatgac gaactaaatt taaaaagttc gctggtaatt gacccccata ttcaccatct
     8821 acgtttaacg gcatatcgct aaaggaagaa atatttaccg attgcgcttt atggtaatgt
     8881 actttcggat gtttaatatg ttcaccgcgt gaggcaagtg tcataatatg tcccaattct
     8941 gcaatatttg ccttttcaac aatcaacagt gtaaacatac cgtcatccaa cttcgcatct
     9001 ggtactaact tctcaaaacc agccattgaa ttggttaagc cgagtaagaa caacatgatt
     9061 tcaccttcaa agacttgcga atcaaactca atccgcacat cgactgcatg catttgaggt
     9121 aacatttcaa atcctttaat atagtacgca aaaggtccga ctatcgattt taatttactc
     9181 ggtgcttcat acgaaacttc cgtaatttta ccaccgccag ctaaattaat aaaataacgg
     9241 ctattcattt taccaatatc aacttgaaca gttttaccat ctaaaatgac atccacggct
     9301 tcaaaaatat cagttgggag atgtagtgct cgaccgaaat cattcaccgt ccccattgga
     9361 attaaaccta acttaggacg attcggtttt tcagcaatac cattcacgac ttcattcaat
     9421 gtaccgtctc caccagcgac aattaacaaa tcgtattggg cctcaatcgc acgcgccgcc
     9481 tcttctgtcg catcgccaac cttttgagtc gcataggcac tcgtctcata accagcttgt
     9541 tccattttaa ttagtacatc aggtaatgca cgtttaaaca cttccttacc cgatgtagga
     9601 ttatagatta ttctcgcacg ttttctcatt tatatccctc aatttttcag tactcacaac
     9661 ttatattata gtaaacaaag atgtaatgaa agctaaaaac aaccaaaagc gtaaaaatga
     9721 taagaaaata acgtcaatcc cgttttcaaa aggcgattgc gtcatatgtt gacatttcaa
     9781 ctttcctagc caagcaaaga aattgagtta cttcatcatt ctttcaccac tacacctaag
     9841 atcaattaat aatcatataa ataaaatgag tgaaaaaatg catgcgtatg attaatacat
     9901 gcgccgtctg tttggtaaga gttattcgtc atttcaactc gtttgagcac cgcaactaag
     9961 attgctagaa caagctcgct ttcattcctt gttacgcaac tgattaccat tcagggcgcg
    10021 actcctgagg gaaaagatgc gccggaaatc catcaacgcc tccaccttat aacagtttca
    10081 gttaagcgtt gattacttgg gaacagccct aggaaagcaa accctaaaag gcaatccata
    10141 cagatattca cattatgaaa gcgagcccta gttctgcgat tgcaaggtgt tataatcaat
    10201 acgacatctt ccgtcatact caaaaaacaa aatgtcacaa aaaagacgag tgtgaaatac
    10261 aatcatcacc attgcattcc atctactcgc ctctattcaa aacaacctat tgtttatcta
    10321 actcttgctt caacaactga tttgcaagct gtggattcgc ttgacctttc gacaatttca
    10381 taatttgtcc aactaagaag cccatcgcct tacctttccc attcttataa tcttcaacag
    10441 attgtggatt ttcagcaatt gcttgttgta caaattctaa cacagcacct tcatcagaaa
    10501 tttgaacaag acctttatct ttcataattt gctttgcgtc cccaccattt tgcgctaatt
    10561 ctgggaacac ttttttcgcc attttactac tcattgtgcc atcttcaatt aatttaatca
    10621 tacctgctaa gtttgctggt gtaaggcctg tatcttgtaa ctcaatttga tttttattga
    10681 gatattcgtt tacgccaccc attaaccagt tcgaaatcat tttcacatct gcgccttctt
    10741 caacagccgc ttcaaagaaa tctgacatct cttttgttaa tgttaaaaca tgtgcatcat
    10801 atgcaggtaa accgaattct tttacgtatt tttctttacg tgcatctggt aattcaggaa
    10861 tcgattcacg aacacgcgct ttccatgctt catcgatata taaaggtacg atgtcaggtt
    10921 cagggaagta acggtagtca tctgacgctt ccttaacacg cattaaaatc gttttacctg
    10981 tggattcatc aaaacgacgt gtttcttgta aaatttcgcc accatttaac aattcttctt
    11041 cttg
//
