LOCUS       NZ_JAQESL010000041     10339 bp    DNA     linear   CON 29-FEB-2024
DEFINITION  Coprococcus sp. AM97-35 AM97-35.Scaf41, whole genome shotgun
            sequence.
ACCESSION   NZ_JAQESL010000041
VERSION     NZ_JAQESL010000041.1
KEYWORDS    WGS; RefSeq.
SOURCE      Coprococcus sp. AM97-35
  ORGANISM  Coprococcus sp. AM97-35
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Coprococcus.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Lin,X., Hu,T., Chen,J., Liang,H., Zhou,J., Wu,Z., Ye,C., Jin,X.,
            Xu,X., Zhang,W., Jing,X., Yang,T., Wang,J., Yang,H., Kristiansen,K.,
            Xiao,L. and Zou,Y.
  TITLE     The genomic landscape of reference genomes of cultivated human gut
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14368)
  AUTHORS   Zou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2022) BGI Reaserch, BGI-Shenzhen, Beishan
            Industrial Zone, Shenzhen 518083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: MGI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/29/2024 10:49:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,361
            CDSs (total)                      :: 3,321
            Genes (coding)                    :: 3,266
            CDSs (with protein)               :: 3,266
            Genes (RNA)                       :: 40
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 6 of 55
            Pseudo Genes (incomplete)         :: 54 of 55
            Pseudo Genes (internal stop)      :: 2 of 55
            Pseudo Genes (multiple problems)  :: 5 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:11:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4029
            Orig. end                         :: 14368
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQESL010000041.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10339
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..10339
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10339
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:>10339](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..>10339
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     gene            85..486
                     /locus_tag="PG840_RS15805"
     CDS             85..486
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025745.4"
                     /locus_tag="PG840_RS15805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4418 family protein"
                     /protein_id="WP_118263138.1"
                     /transl_table=11
                     /translation="MKKKVRVMLNCMVILAMVSGFTVFPVCSDFLELSNGNMAYMSCHY
                     MMKMGILVSVLFVAVIVEQFLNKKNFKVLYIVFGVILILLTLHSPIFDGPCRNVMMDCH
                     KTAWCFRMAGGISLIIGCLQFTKEKGNRV"
     gene            486..1664
                     /locus_tag="PG840_RS15810"
     CDS             486..1664
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /locus_tag="PG840_RS15810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_118263137.1"
                     /transl_table=11
                     /translation="MIKLVLSNIFRKKKDTILTGTILGIAVFTLVLIGCTYYQVHEGIK
                     LTNDRLGADVIILPRTDKIDYSSALYTAEPSEEYIYLSNLEFLHGDYKIEDKTYQFFTH
                     TIKAGCCTVTEEARVVGFDQATDFIIKPWLEMQEFDILADDEIIIGGDITSVIGQRMSI
                     LGKTYKIVGTLYFTGSGLDQSIFMDIEEARRLTENSFFQNDVDKKVSSVLLRMKEGVNI
                     DDYVQNFNMNNPELTAISKSEALSYVKNQLLGWGQIVGILIVAMSIILIIALFNRYTAI
                     IQNRKRELGYLISLGMSRKEICISIVGEISILVILYGGIAGGTALLCIKPLVNRLKDFF
                     DFPISVIGINEYIFALSLGIGFAFVVSIMACILPLIRILKQDPQELFSIYNG"
     gene            1693..2364
                     /locus_tag="PG840_RS15815"
     CDS             1693..2364
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="PG840_RS15815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_118263136.1"
                     /transl_table=11
                     /translation="MGEVICELKKVSKKFKKSDCCPIKKLDMKINAGDYISIEGVSGIG
                     KSTLLYLIGGLISPTEGDIFYFGKNVKSEKDFLSIRKKSISYIFQDYRYIEFFTIEENL
                     MFAARNRKLCKEKIKEEMEYYLEILHLQDKRRVYPYNLSGGQKRRLMIAIAMIRDTQIL
                     IADEPTNDLDLFMEEKVLNLFDDKVKEGKTIILSTHNPRVSQRVNLRYQIEASRLSILG
                     G"
     gene            2424..3131
                     /locus_tag="PG840_RS15820"
     CDS             2424..3131
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PG840_RS15820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_118263135.1"
                     /transl_table=11
                     /translation="MKKTLKGILVTGLAAGMCLSSTVTSFAAENDPAEGKIYTLSTTYF
                     DITSLPDEVVDLYEKSGWIINEDYSYRKTHLTTGKLWVNGNEATINTDGSFEVPQDVDT
                     ITIKYTADGEEQIISKNEEGDFEVVNAVNLESLMDRMDTIASETTASARKGYGDKYYPG
                     DWVHCNRFNGPFSDGVHYAKTNPKAYTNFISSDCDIALANSTVCWGWDYCNQSGPAAGC
                     SIEIGHSTKYHKH"
     gene            3181..3699
                     /locus_tag="PG840_RS15825"
     CDS             3181..3699
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PG840_RS15825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_118263134.1"
                     /transl_table=11
                     /translation="MKKKVAIICILVVLIVTGVGVILNKNISYNAFINKHFSQEFLVKS
                     SRVQKEIRTEILENVFDIVNLEKQDVKNIQISDETEEQLLKKVWELEVYIEKVKIDDLS
                     KADQERFLEFKKNSIENLKELYRLIDEYNEKTIQNKNITSNYYFELQRKLTSPIESING
                     YIMLNELKK"
     gene            complement(3771..4856)
                     /locus_tag="PG840_RS15830"
     CDS             complement(3771..4856)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842240.1"
                     /locus_tag="PG840_RS15830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_246944719.1"
                     /transl_table=11
                     /translation="MSILISIFISGYHGKTTDFAKNSSCHRTTIAHFLNSGKWDDSLLS
                     DTLKCSVIEIIYSEAARTGKPAFCIVDDTIASKTKPSSQALHPIEDAYFHQSHLKGKQD
                     YGHQAVAVMLSCNGIVLNYSFVMYNKSISKIDIVQSIAKELPVPPVMSYFLCDCWYVSE
                     KIINTFAQRGVHTIGALKTNRLLYPSGMKKKLRELAAELSVTHREFDLVTVKKQNDYVY
                     RYEGNLNGIENAVVLLSYPEKAFGNLKALRAFISTNVALSTQEILSWYVCRWPIEVFFR
                     QCKDKLALDGYQIRSAQGIKRYWLLMSLAHFMCAVGTGRFCSFETGYHEICDTIQLEKY
                     RYLFQCAKESNDFDSFMKFAV"
     gene            5258..5446
                     /locus_tag="PG840_RS15835"
     CDS             5258..5446
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PG840_RS15835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_118263133.1"
                     /transl_table=11
                     /translation="MTVLITKEKAELFVAAHEDKNVSRNESDGYPKTDHKYMDLNNKAG
                     RVKYMKVSEDGMTDIIY"
     gene            5755..5997
                     /locus_tag="PG840_RS15840"
     CDS             5755..5997
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PG840_RS15840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_118263132.1"
                     /transl_table=11
                     /translation="MQDYKNIKRYKLSLSESKVIRQLGGSESGVNAFLRAFEKCEVLTY
                     RIRRYHELRDSRTVSKSGGRIKTLNILAMNQMPSS"
     gene            6111..6836
                     /locus_tag="PG840_RS15845"
     CDS             6111..6836
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1026: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /locus_tag="PG840_RS15845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="IS3 family transposase"
                     /protein_id="WP_270613862.1"
                     /transl_table=11
                     /translation="MYDDSKQNYGAPKITVELHKTGEVISERTVGTYMRQMGIRAQWSK
                     PWTITTKDSDFSTELENILDEQFHPDRPNAVWCSDITYIWTIDGFVYLTSIMDLFSRKI
                     IAWTLSETLEVSCVIDTINKAKARRNIDQPLIIHSDRGSQYVAKEYKKATENMQRSYSK
                     KAFPWDNACMESFHSIIKREWLNRFKIRDYKQAYRLIFEYLEAFYNTKRIHNYCDYMSP
                     DEFERVYERTHIKVELLAS"
     misc_feature    complement(7321..7336)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.57"
                     /tool="antismash"
     gene            7645..7989
                     /locus_tag="PG840_RS15850"
     CDS             7645..7989
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /locus_tag="PG840_RS15850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_118263130.1"
                     /transl_table=11
                     /translation="MESRVKELRLARRLSQEMLGQSLGITQQTISKIENGKYDIPTDLL
                     ISLSNFFNVTTDYILGLSDIKRNLEGQIRVNKTLDDNYDLVMQYNELDSTNKKTLDILL
                     SRLCETQKEK"
     gene            7991..8719
                     /locus_tag="PG840_RS15855"
     CDS             7991..8719
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="PG840_RS15855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_118263129.1"
                     /transl_table=11
                     /translation="MVKIAICDNDIVLTSLVENLLYESAQKINIPIEIDIFFDGVTLKA
                     SMDQGTNYDLIYLDIEMEKLDGIKTARYIRKKNCTVLIIYISSYEKYLKELFEVEPFRF
                     ISKPIDTMIFFKYFCSAYERIESGQEQFEIKFKNEIEHVPVKEIVYFESEKRYVHVITD
                     KEKYTFYGKLKEVEKRVNSGKFLFIRIHQSYLVNYKYIKKITLLSVELYNGIKLRISED
                     RVKEVRKKFFEIAKGSIADV"
     gene            8712..10004
                     /locus_tag="PG840_RS15860"
     CDS             8712..10004
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="PG840_RS15860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_118263128.1"
                     /transl_table=11
                     /translation="MFDVFMNIMLSVFSLYIVESYFSIYFAKSEKGIKVHMASLGYVVF
                     QYFSDLLSLFSPVILFVLNIIVVTLVVRWKYKGLFSTQIMMSILLLIIWMISEVLVGYI
                     FKYKGLSYEQVEVSGSVISKIIILIFVKIMKTKKNWAGQSVSLEVSIGLLCIPVSSIYI
                     VHNIFALCSELESGFAFPFVSSIIILGMNCMVFIIYDKLALEEELYRNNLLFTQQLDFN
                     ERQMRERELSVQEFKRLRHDMKSHLFSIKAFLQNQQVEKAVLYIDNIANSGGLSSKDVC
                     KSGHIIIDALINYRCSIAEEKDIKCNIVHQIPTELYFRNSDICVLLGNAFDNAIEAAEK
                     VEISERYIELRMGYNKGCLLIEISNSYNGTIKMGNKGEFMTTKQDQKNHGIGMVSMKKV
                     VDTYNGEMTINAENNIFEMTIMLYEREGERL"
     gene            10001..>10339
                     /locus_tag="PG840_RS15865"
     CDS             10001..>10339
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /locus_tag="PG840_RS15865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_270613861.1"
                     /sec_met_domain="AgrB (E-value: 5.5e-24, bitscore: 74.7,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKISHHIVMYAIAKGEVDEKDYDIYKYGFWIGIELCISIIVSV
                     LCAISLDSLIELILFLSIFAPLRAFAGGIHLKTFKNCFLLSIGSLILILSIVKYCNLPV
                     MLLNWGILC"
ORIGIN
        1 catcaattat cgcatttttt acattagtaa tagaaataga ttcatttatg ataggatata
       61 attaaaaaca aggagaaaaa agagatgaag aaaaaagtgc gagtaatgtt aaattgtatg
      121 gtgattttgg ctatggtgag tggtttcaca gtttttccag tgtgtagtga ttttctggag
      181 ttatctaatg ggaatatggc gtatatgtca tgtcactata tgatgaaaat gggaatattg
      241 gtaagtgtac tttttgtagc agtgattgta gagcaatttt tgaataaaaa aaatttcaaa
      301 gtgctttata ttgtgtttgg agtgatttta atacttttaa cattacattc accgattttt
      361 gatggaccat gtagaaatgt tatgatggac tgtcataaaa cggcatggtg ttttagaatg
      421 gctggcggaa tatctttaat cataggatgc ttgcagttta caaaagaaaa ggggaataga
      481 gtgtaatgat aaaattagtt ttatccaata tttttagaaa gaaaaaagat actattctta
      541 ctggtacaat cttggggata gcagtattta ctttggtatt gataggatgt acgtattatc
      601 aagtgcatga agggatcaag ctaacaaatg atagattggg tgctgatgtg attattttac
      661 caagaacaga taaaattgat tattcttctg cactttacac ggcggaacct tctgaagaat
      721 atatatatct ttctaattta gaatttctac atggggatta taaaattgaa gataaaacat
      781 atcaattttt tactcatacc attaaagctg gatgctgtac ggtgacagaa gaggcacgag
      841 tagtaggatt tgatcaagca accgatttta tcataaagcc atggctggaa atgcaagagt
      901 ttgatatttt agcagatgat gaaattatca taggcggaga tataacatcc gtgattggac
      961 aaagaatgtc tattcttgga aaaacataca aaatagtggg aaccctatat tttacaggaa
     1021 gtggattgga tcaaagtatt tttatggata ttgaagaggc aagaagactt acagagaaca
     1081 gtttttttca aaatgatgtt gacaaaaaag tctcatcggt attgttaaga atgaaagaag
     1141 gagtaaatat tgacgattat gttcaaaatt ttaatatgaa taatcctgaa ttgactgcga
     1201 tttctaaatc agaggctttg tcttatgtaa agaatcaatt attaggatgg ggacaaatag
     1261 taggaatttt aatcgttgct atgagcatca ttcttataat agcactcttt aatcgatata
     1321 cagcaataat ccagaataga aaaagggaac ttggatattt aatttcgctt ggaatgagca
     1381 gaaaagaaat atgtatttct atagttggtg aaatttctat tttagttatc ttatatggtg
     1441 gcattgctgg tggaacagct ctattatgta taaaaccgtt ggttaatagg ttgaaggatt
     1501 tttttgattt tccaatttca gtaataggaa taaatgaata tattttcgct ttgagtttgg
     1561 gaattggctt tgcatttgtt gtgagtatta tggcatgtat acttcctttg attcgaatat
     1621 taaaacaaga cccccaagaa ttattttcga tatataacgg ataaaaagaa gtggatgagg
     1681 tgaaaagaaa caatgggaga agtaatatgt gaattgaaaa aagtaagcaa aaaatttaaa
     1741 aaaagtgact gttgcccaat aaaaaaattg gatatgaaaa taaatgcagg agattatatt
     1801 tcaattgaag gtgtgtcagg aatagggaaa agtacattgc tatatttaat aggtggttta
     1861 ataagtccaa cagaagggga catcttttat tttgggaaaa atgttaaatc cgaaaaagat
     1921 tttttatcaa tccgaaaaaa aagcattagc tatatctttc aagattatag atacattgaa
     1981 ttcttcacaa tagaagaaaa tttaatgttt gcagcaagaa atcgaaaatt gtgcaaagaa
     2041 aaaataaaag aagaaatgga atattatcta gagatactac atttgcaaga taaaagaagg
     2101 gtatatccat ataatttaag tggtggacaa aaacgtcgtt tgatgattgc aattgcaatg
     2161 attagagata ctcaaatact tatagcagat gaacctacta atgatttgga tctttttatg
     2221 gaggaaaaag ttttaaatct ttttgatgac aaagtaaaag aaggaaaaac tattattttg
     2281 tctacgcata acccaagggt atcacaaagg gtaaacctta gatatcaaat agaagcttct
     2341 agattatcga ttttaggggg gtgatattca tttaggaatc aagtagtcgg taaaaataat
     2401 tctaagtaga aagagggaaa aaaatgaaga aaacattaaa aggtatttta gtaactggtt
     2461 tagctgcagg aatgtgttta agtagcacag tgacatcttt tgcagcggaa aatgatccag
     2521 cagaagggaa aatttatacg ttatcaacta catattttga tattacatct cttcctgatg
     2581 aagtggtgga tctttacgaa aaaagtggat ggataattaa tgaagattat tcatacagaa
     2641 aaactcattt gacaacagga aaattgtggg taaatggtaa tgaagcaacg attaacacgg
     2701 atggttcatt cgaggtgcca caggatgtag acactattac aattaagtat acagcggacg
     2761 gagaggaaca gattatcagt aaaaatgaag aaggtgattt tgaggttgtc aatgcagtaa
     2821 atttagaatc tttgatggat agaatggata ccattgcatc tgaaacaaca gcatctgcta
     2881 gaaaaggcta tggagataag tattatccag gtgactgggt acattgtaat cgatttaatg
     2941 gtcctttttc tgatggtgta cattatgcta agacgaatcc taaggcatac accaatttta
     3001 taagtagtga ttgtgatata gctttagcta attccactgt ttgttgggga tgggattact
     3061 gtaatcaaag tggtcctgca gcaggttgtt ctattgaaat tgggcattct acaaaatatc
     3121 ataaacatta aaaagattgg taaagcgggc attttaggat gcccgctgta aggtgacgtt
     3181 atgaaaaaaa aggtggctat aatttgtatc ttagtagtgc taatagtaac gggagtagga
     3241 gtgatattaa acaagaatat ttcttataat gcttttataa acaagcattt ttcacaagaa
     3301 ttcttagtaa agtcaagccg ggttcaaaaa gaaatacgaa cagaaatatt agaaaacgta
     3361 tttgatatag ttaatttaga aaagcaagat gtaaagaata ttcaaatatc tgatgagaca
     3421 gaagaacagt tattaaaaaa agtttgggaa ttagaagtat atattgaaaa agtaaaaata
     3481 gatgatttgt ctaaggctga tcaagagaga tttttagaat ttaaaaaaaa ttccatagaa
     3541 aatcttaaag aactttatag actgattgat gagtataatg agaaaaccat acagaataaa
     3601 aatataacaa gcaactatta ctttgagttg caaagaaagt tgacttcacc cattgaatct
     3661 ataaatggtt atattatgtt aaatgagttg aaaaaataat aatattagaa ctaattgatg
     3721 aattaaatta tacgttctat aaatgagcaa atttgaaaaa ttgcacaaaa ctacactgcg
     3781 aatttcataa atgaatcaaa atcattactt tcctttgcgc attgaaaaag ataacgatac
     3841 ttttccagct gaatggtatc acagatttcg tgatatccag tttcaaacga acagaaccta
     3901 ccagtaccca ctgcacacat gaaatgtgcc agtgacataa gtagccagta ccttttgatt
     3961 ccctgtgcag agcgtatctg atagccgtcc agtgccagtt tatccttaca ctggcggaaa
     4021 aatacttcaa taggccatcg acacacatac caggaaagaa tctcctgtgt agatagggct
     4081 acgtttgtac tgatgaaagc acgcaatgct ttgagattac caaatgcttt ttccggataa
     4141 ctcaaaagaa ctaccgcatt ttctatgccg ttgaggtttc cctcgtaccg gtacacataa
     4201 tcgttttgtt ttttgactgt cacaaggtca aattcacgat gtgtaacaga caattcggcg
     4261 gccagttcac gaagtttctt tttcattccc gatggataca gcaaacggtt tgttttcaaa
     4321 gcaccgatgg tatggacccc tctttgtgca aaggtattga ttatcttttc agaaacatac
     4381 cagcagtcac aaagaaaata ggacattacc ggtggaacag gcagctcctt tgcaatgctt
     4441 tgtacaatgt caatcttgga aattgactta ttgtacatta caaaagaata gttcagaaca
     4501 atgccattgc aggaaagcat aacagcaact gcctgatgcc cgtaatcctg ttttcccttt
     4561 aaatgggatt ggtgaaaata cgcatcttca atcggatgta aagcctgtga cgaaggcttt
     4621 gtctttgaag ctatcgtatc gtccacaata cagaaagcag gctttccggt gcgtgctgct
     4681 tctgaataaa taatctcaat gacagagcat tttaacgtat ctgaaagtaa tgaatcatcc
     4741 cattttccgg aattgaggaa atgggcaatc gtagttctgt ggcaggaact gtttttagca
     4801 aagtccgtgg tttttccatg atatcctgaa atgaaaatac tgattaggat actcataaga
     4861 tggtttatta ttcggttcga ataaaatttg cataagttta attttttaaa ctggttgtat
     4921 atgaatgagg aatgatgtat agtatttgcg agagacacct tctttcgtaa atgttgcaaa
     4981 tgtttgttta ggcactttca ttatacaaca cacggaatcg aggtgtcttt cttttatgca
     5041 aaatcacgaa cttgctcatt tatagttata cgttaatagg atgctgtaaa agagtgaatt
     5101 aaaaaggttc cgaaaaaaga ttatgcattc gatattcttt cgatgctcta aaagtgaaga
     5161 ctacaaaaaa atcaatatca tagactgttg caatgtttag ttcaaacagt tgggggatag
     5221 aagtgatgct ttttggaatg gactgtggaa tgcggagatg actgtactga tcactaaaga
     5281 aaaagcagaa ttgtttgttg cagcacatga agataaaaat gtgtcaagga acgaatccga
     5341 tggttatccc aaaacagatc ataagtatat ggacttgaat aataaggctg ggcgtgtaaa
     5401 gtatatgaaa gtgtctgaag atggaatgac agatatcatt tattaaaata tttattcaac
     5461 aacagagttt gtatagctac acaagtaata aagactatta gaattcaaaa cttaggggtt
     5521 aaaaatatgt ggtatatata aagtttaaga ggataaacag tgcggttttg cactttctta
     5581 tttgtgtaat tgaatacttt catatagcac actcgaacgg ttgggattgg gaaacaaaaa
     5641 gatcgaaatg cgaagttaaa cagacgccgt acgtctgcct agtacagcaa ccttcgagat
     5701 gataaatgta ttcattcgta gggagaaaat acatttatta cagggggata atgtatgcag
     5761 gattacaaaa acatcaaacg ctataaactt tccttatccg aaagcaaagt catcagacaa
     5821 ttgggtggaa gtgaatcagg agttaatgca ttcctaagag catttgaaaa gtgtgaggtt
     5881 ctgacttatc gtatacgccg gtatcacgaa ttacgtgata gtagaactgt atccaaatca
     5941 ggtggaagaa tcaagacatt gaacattcta gcaatgaatc agatgccaag ttcatagcaa
     6001 catgaaaaaa tatgacgatt gttttccttt ggaacagatg ctctaagaag tgtaccgtac
     6061 cgaagacgga aaaagtttct actgatgtta gaatataaat agtacaggat atttatgatg
     6121 attctaagca gaactatggt gctccgaaaa tcacggtaga gctgcataaa accggtgaag
     6181 ttatttcgga aagaacggtt ggcacctata tgcgccaaat gggaatccgc gcccaatgga
     6241 gcaagccatg gacgatcacc acaaaagatt ctgatttcag cacagaatta gaaaatatcc
     6301 ttgatgaaca gtttcatcct gatcgcccga atgctgtttg gtgttcggat atcacctaca
     6361 tctggacaat agacggattt gtctatctga ccagtattat ggatttattt tccagaaaga
     6421 tcattgcctg gacactttca gaaacattgg aggtatcttg tgtaattgat actataaata
     6481 aagccaaagc tcgtcgaaat atcgatcagc cgctaatcat tcactcggat cgggggagtc
     6541 agtacgttgc aaaagaatat aaaaaagcaa ctgaaaatat gcagcgtagt tattcaaaga
     6601 aagcatttcc gtgggataat gcgtgcatgg aatccttcca ttctattatt aaacgcgaat
     6661 ggctgaaccg ctttaaaatc cgtgattata aacaggcgta tcggttgatt ttcgaatatc
     6721 tggaagcttt ctacaatacc aaacggattc ataactattg cgactatatg tcaccggatg
     6781 aattcgagcg agtatatgaa agaacacaca taaaggttga gcttctggca agttaaaata
     6841 tggagaaatt tctcatttta acttgtacta aatcttgaca taggaccata caagtatccc
     6901 caatatttca aactctaacc ggttttataa atgtgacgtt ccaaagtaga aggcttggct
     6961 tttctaagaa cttgtgatac agtattttac gaaatacgac aggtactagc aattgcacgt
     7021 tcgctgaagg ctaaactatg tagttttaga atttcacgat attgggttat ggggagattt
     7081 tctttgaatg tgtttatgcc caaaattaag cgtattaata cagtacaaat ggtttatgta
     7141 agtgtggctc tgaggagtcg agaatactta attaacaaaa gaaaagtttt ggtataataa
     7201 accgaagaga aattattaca ttaaaagcgg gaattattac acttacaaaa atatttgcga
     7261 aaaatagagt aatataagga aaagaaataa atgaaaggat aaagcaacag atgaaaaagt
     7321 gtgtaaaaaa agtaaatatg atgagttctg ttactacttt ggcattatta attaccacat
     7381 ttgcggcgaa tatgaaatgt gtttatattt tccatcagcc agaacagaat gcaaaaataa
     7441 aaactttaag aaaattttaa gaaaaagcag gcgtgtttaa acatgtctgc ttttttgaaa
     7501 atgtgaaaat aaaaaatatg aattttagaa aatggtatct ccaaaaaccc tcaataagaa
     7561 tattacaata ctgtttatat gagtaattat aaaaagaata aacgataaaa tataatggaa
     7621 tattattatt gaggagtcat ttatatggaa agcagagtaa aagaacttag acttgcaaga
     7681 cgcctaagtc aggaaatgtt aggacaaagt cttggaatta cacaacagac aatcagcaaa
     7741 atagaaaatg gtaagtatga tattcccacc gatttactaa tcagcttgtc aaattttttt
     7801 aatgttacaa cggattatat attaggtttg tcagatataa aaagaaattt ggaggggcaa
     7861 atacgtgtca ataaaacttt ggatgataat tatgatttag taatgcaata caacgaattg
     7921 gatagcacga ataaaaagac gttagatata cttttaagta gattatgtga aactcaaaag
     7981 gagaaataat atggtaaaga ttgcaatttg tgataatgat attgtattaa cttctcttgt
     8041 agaaaatcta ctgtatgaat ctgctcaaaa aataaatata ccgattgaga ttgatatctt
     8101 ttttgatggt gtaacattaa aagcaagcat ggatcagggg actaattatg atttaatata
     8161 tttggatata gaaatggaga agttagatgg tataaaaact gctcgatata taagaaaaaa
     8221 gaattgtact gttttgatta tttatatttc tagttatgaa aagtatttaa aagaactatt
     8281 cgaagtagag ccattcagat ttattagtaa gccaattgat acgatgatat tcttcaaata
     8341 tttttgtagt gcatatgaaa gaattgaaag cggtcaagaa caatttgaaa ttaaatttaa
     8401 aaacgagatt gagcatgttc cagtaaaaga aatagtatat tttgaaagcg aaaaaagata
     8461 tgtgcatgtt atcactgaca aagaaaagta tacattttat ggtaagttaa aagaagtaga
     8521 gaaacgggtt aacagtggaa aatttctttt tataagaatc caccaatcgt atttggtaaa
     8581 ttacaaatat attaaaaaga ttacactttt atcagttgaa ctctataatg gtataaaatt
     8641 acgtattagc gaagatcgag taaaagaagt gcgaaaaaaa ttttttgaaa tagcaaaagg
     8701 gagtatcgct gatgtttgat gtgtttatga atataatgtt gtcagtattt tcgctgtata
     8761 ttgtggaatc atatttttcc atatattttg caaaatcaga aaaaggaatc aaagttcata
     8821 tggcatctct tggatatgta gttttccaat atttttctga tttattaagc ctattttcac
     8881 cagtcattct gtttgtattg aatataattg tagttacatt ggttgttcga tggaaataca
     8941 aaggactttt tagcacacaa atcatgatgt ctattctttt gttgataata tggatgattt
     9001 cagaagtttt agtgggatac atttttaagt acaaaggact atcctatgag caagtagaag
     9061 tgtcaggttc agttataagt aaaatcatta tactcatttt tgttaagatt atgaaaacaa
     9121 agaaaaattg ggctggtcaa tcggtttcat tagaagtaag tataggactg ctatgtatac
     9181 cagtaagtag tatttacatt gtacataata tttttgcctt atgcagtgaa cttgaaagtg
     9241 gatttgcctt tccgtttgtt tcttctatta taattctggg catgaattgt atggtattta
     9301 taatatatga caaattagca ttagaagaag aactttatag aaataatttg ttgtttaccc
     9361 agcaattaga ttttaatgaa agacaaatgc gggaaaggga attatctgta caagaattca
     9421 aaagattacg ccacgatatg aagtcacatt tattcagtat aaaagctttt ttgcaaaatc
     9481 agcaagttga aaaagctgtt ttgtatatcg ataatattgc aaattctggt ggtttatctt
     9541 caaaagatgt gtgtaagagt ggacatatta taattgatgc attgattaat tatagatgct
     9601 caattgccga agaaaaagac ataaaatgca atattgtcca tcaaattcct acagagttat
     9661 attttagaaa tagtgatata tgcgttttgt taggaaatgc tttcgataac gccattgaag
     9721 cagcagaaaa ggtagaaatt tcagaacgct atatagagct gagaatggga tacaataaag
     9781 ggtgtcttct tatagaaata tcaaattctt ataatggcac tatcaaaatg gggaataaag
     9841 gggaatttat gacaacaaag caagatcaaa aaaatcatgg aatagggatg gtatctatga
     9901 aaaaagttgt tgatacatat aatggagaaa tgactataaa tgcagaaaat aacatttttg
     9961 aaatgacaat tatgttatat gaaagagaag gagagcgact atgattgaaa aaatttctca
    10021 ccacatagtt atgtatgcta ttgcaaaagg tgaagtagat gaaaaagatt atgacattta
    10081 taagtatgga ttttggatcg gaattgaatt atgcattagt attattgtaa gtgtgttgtg
    10141 tgcgattagc ttagatagtt taatagaatt aatattattt ttgagtatat ttgctccact
    10201 tagagcattt gctggtggaa ttcatttaaa aacatttaaa aactgttttt tattatccat
    10261 tggtagtttg atattaattt tgagtattgt aaaatattgt aatctaccag ttatgttatt
    10321 aaattgggga atattatgt
//
