LOCUS       NZ_JAQCYD010000007     10406 bp    DNA     linear   CON 23-FEB-2024
DEFINITION  Streptococcus mutans strain AF36-21BH AF36-21BH.Scaf7, whole genome
            shotgun sequence.
ACCESSION   NZ_JAQCYD010000007
VERSION     NZ_JAQCYD010000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus mutans
  ORGANISM  Streptococcus mutans
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 85286)
  AUTHORS   Lin,X., Hu,T., Chen,J., Liang,H., Zhou,J., Wu,Z., Ye,C., Jin,X.,
            Xu,X., Zhang,W., Jing,X., Yang,T., Wang,J., Yang,H., Kristiansen,K.,
            Xiao,L. and Zou,Y.
  TITLE     The genomic landscape of reference genomes of cultivated human gut
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 85286)
  AUTHORS   Zou,Y., Lin,X. and Hu,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) BGI Reaserch, BGI-Shenzhen, Beishan
            Industrial Zone, Shenzhen 518083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: MGI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/23/2024 01:23:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,988
            CDSs (total)                      :: 1,943
            Genes (coding)                    :: 1,890
            CDSs (with protein)               :: 1,890
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 33 of 53
            Pseudo Genes (incomplete)         :: 28 of 53
            Pseudo Genes (internal stop)      :: 23 of 53
            Pseudo Genes (multiple problems)  :: 22 of 53
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:29:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 40432
            Orig. end                         :: 50838
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQCYD010000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10406
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10406
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10406
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[<5000:5406](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <5001..5406
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            18..1202
                     /locus_tag="PGX70_RS08845"
     CDS             18..1202
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302863.1"
                     /locus_tag="PGX70_RS08845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_270513872.1"
                     /transl_table=11
                     /translation="MKAFMEKFSLLSLSLMLVSTFAVSPALPKMLSFYQTRGYSNAQVE
                     LLLSLSSFAILAVLLLNPWINLWLSERWSIILGLLLLSIGGILPVFLQFYPLVFLSRLL
                     LGLGIGLINARAISMISERFSGKIRVQMLGLRGSAEVLGSAILTFLAGQWLNVSWSFAF
                     LIYGFGFLILLCYLLFVPAAKEKRESSQKQMYSTASKNHFTFRQLLYIFGLDLYAGFVI
                     LVNSANTLRIPLVVEKLHLATASQASFILSLMMLMGILAGTLFSFLLALFKDYLMPLVV
                     FVLGLGMLILWQANHLWLLSLGALVTGFVYSLGVTLVFHLLSEHMHRKQLTTATTLILI
                     GCNLGGGCASFVLQLFACINSDVKAAFLIFAVLSLILGTLLLIRVLKTNRNLRK"
     misc_feature    complement(1307..1322)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.06"
                     /tool="antismash"
     misc_feature    complement(1329..1344)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.4"
                     /tool="antismash"
     gene            1402..4071
                     /gene="adhE"
                     /gene_synonym="adhC"
                     /locus_tag="PGX70_RS08850"
     CDS             1402..4071
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /GO_function="GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /GO_function="GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006066 - alcohol metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="adhE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /gene_synonym="adhC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228226.1"
                     /locus_tag="PGX70_RS08850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="WP_002264767.1"
                     /sec_met_domain="Aldedh (E-value: 3.1e-20, bitscore: 64.5,
                     seeds: 89, tool: rule-based-clusters)"
                     /sec_met_domain="Fe-ADH (E-value: 6.8e-116, bitscore:
                     379.3, seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKETKTEALTAEEAAKQYTNSLVEKALKAEKIYATYTQEQVDKI
                     VAAMALAGSEASLQLAKEAHEETGRGVVEDKDTKNHFATEYVYERIKNEKTVGIIGEDK
                     VAGSIEIAAPLGVLAGIVPTTNPTSTTMFKILVALKTRNAIVFAFHPAAQKCSAHAAQI
                     LYDAAVKAGAPENIVQWIETPSIANTGALISNKGIASILATGGPGMVNAALKSGNPSMG
                     VGAGNGAVYVDATANLERAVEDLLLSKRFDNGMICATENSAVVEAPIYKEWLAKMQEKG
                     AYLVPKKDYKKFEDFVFNDNHGVNGPVAGMSAKWICEQAGVTLPEGKDVLLFELDKKKI
                     GEKLSSEKLSPLLSVYKAKDRKDGIDIVAALLDYQGAGHNCAIQIGSQADPFVAEYGDA
                     LNSSRVLVNQPDSVGGIGDVYTDALQASLTLGTGSWGKNSLSHNLSTGDLLNVKTVAKR
                     RNRPQWVRLPEKTYYEKNAISYLQDETETMTRAIIVADPGMLQFGFVDTVLAQLSLRDE
                     KVATSIYGTIKPDPTLGQTIEIARQMRDFKPDTVIAVGGGSALDAAKIARYIYEYSLDQ
                     EADFLDSYEKVSELFLELQQKFIDIRKRIVRFKHQTVTRLFCIPTTSGTGSEVTPYAVI
                     TDDFTHVKYPLTDYELTPQVAIVDPEFVMTVPKRTVALSGLDTLSHALESYVSVMASDF
                     TRPWSMEAIRLVIENLEDSYNFDPKNPTLRGEQARENMHYAATLAGMAFSNAFLGINHS
                     LAHKTGGEFGLPHGLAISIAMQHVIRYNGVAGNVKRSVYPRYEEYRAQRDYADIARALG
                     LKGKDDAELVEALCARIDQLMHAVDVEPKLSANGVTKKAFDAAVDRLASLAYDDQCTPA
                     NPRQPYIEEMKQLLIDQF"
     gene            4246..4714
                     /gene="tnpA"
                     /locus_tag="PGX70_RS08855"
                     /pseudo=""
     CDS             4246..4714
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tnpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /locus_tag="PGX70_RS08855"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="IS200/IS605 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MREANESLSHTKWNCSTILFSHSSIIVKSFMADTKPVSEELSGTY
                     VNAKA"
     assembly_gap    4989..4998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4999..5161
                     /locus_tag="PGX70_RS08860"
     CDS             <5001..5161
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019322451.1"
                     /locus_tag="PGX70_RS08860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_270513881.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 8.7e-13,
                     bitscore: 40.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AFEQFDVMDSQTLSTVEGGKVSGGEAVAAIGGLVGATLVTPYCVG
                     TWGLIRSH"
     gene            5191..5406
                     /locus_tag="PGX70_RS08865"
     CDS             5191..5406
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263728.1"
                     /locus_tag="PGX70_RS08865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_002263728.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 7.4e-13,
                     bitscore: 41.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNVNNYKSLTNDELSEVFGGDKQAADTFLSAVGGAASGFTYCA
                     SNGVWHPYILAGCAGVGAVGSVVFPH"
     misc_feature    5510..5523
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 22.61"
                     /tool="antismash"
     gene            5601..6020
                     /locus_tag="PGX70_RS08870"
     CDS             5601..6020
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319845.1"
                     /locus_tag="PGX70_RS08870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_032538836.1"
                     /transl_table=11
                     /translation="MKKLERISTKILRWAYLILTTIVFVVALIIYNWLYYGKSMLFWLP
                     AVLVLLVTALLAYLVKKQLEARLEPLLIFNTLQQIIGILIALAVGLFALAYKALNTKIN
                     DGNEVYWILFAAILLIFSWNAVELYKELKRRSKNK"
     misc_feature    6267..6281
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.18"
                     /tool="antismash"
     gene            6673..6981
                     /locus_tag="PGX70_RS08875"
     CDS             6673..6981
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /locus_tag="PGX70_RS08875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="WP_151416158.1"
                     /transl_table=11
                     /translation="MGDEKLSPGENQSVLIPKSCTIYYLKDWTCQEKVDRKLSELERDG
                     LISRRDYQQMPPKVVYSITDYGRTANTIIDVVCHCREDNIRRRQILGEEIDLISYDF"
     misc_feature    complement(7007..7027)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 26.2"
                     /tool="antismash"
     gene            7361..7630
                     /gene="rpsO"
                     /locus_tag="PGX70_RS08880"
     CDS             7361..7630
                     /GO_component="GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA]"
                     /GO_component="GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA]"
                     /GO_function="GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rpsO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /locus_tag="PGX70_RS08880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="30S ribosomal protein S15"
                     /protein_id="WP_002263008.1"
                     /transl_table=11
                     /translation="MAISKEKKNEIIKQYARHDGDTGSVEVQVAVLTWEINHLNEHIKQ
                     HKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRDLISSLGLRR"
     misc_feature    complement(8383..8396)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.18"
                     /tool="antismash"
     misc_feature    complement(8739..8753)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.05"
                     /tool="antismash"
     misc_feature    8740..8754
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.05"
                     /tool="antismash"
ORIGIN
        1 gtgtttaagg agttgttatg aaagcgttca tggagaaatt tagtctctta tccttatcgt
       61 taatgctggt gtccactttt gccgtttcac cggcacttcc taagatgctt tctttttatc
      121 aaacacgcgg ttacagtaat gctcaagttg aattgctgct ttctttatct tcttttgcta
      181 tattagcggt cttactgctg aatccttgga tcaatctatg gctgtcggaa cgctggtcta
      241 ttattttagg cttgcttttg ctttctatag gcggtatttt acccgttttt ctgcagtttt
      301 atcctttagt tttcctgtcg cgtttacttt taggtttggg aataggtctt attaatgcgc
      361 gagcgattag tatgattagc gaacgttttt caggcaaaat cagagttcaa atgttaggat
      421 taagaggttc ggcagaagtt ctaggatcag ctattttaac ctttttagca gggcagtggc
      481 tgaatgtttc ctggtctttt gcttttttaa tttatggatt tggctttctt attttattat
      541 gctacctctt atttgtgcca gcagctaaag aaaaaagaga gagttcccaa aagcagatgt
      601 attcaactgc ttctaagaat cattttacct tccgtcagct cctttatatt tttggtttgg
      661 atctctatgc tggttttgtt attttggtta attctgctaa taccctacgg attcctcttg
      721 ttgtggagaa gttacatttg gcaacagcca gtcaagccag tttcatttta agtctgatga
      781 tgttgatggg cattttggcc ggcacacttt ttagtttttt gctagcttta tttaaagatt
      841 acctaatgcc gctagttgta ttcgttttgg gtttgggtat gctgatactt tggcaggcta
      901 atcatttatg gcttttaagc cttggagctt tggtgaccgg ttttgtatac agtttaggcg
      961 ttaccttggt tttccatctt ctttcagaac atatgcatag gaaacagttg acaacggcga
     1021 caaccttgat tttgattggc tgtaatttag gcggcggctg tgcttctttt gtcctgcagc
     1081 tgtttgcatg tattaattca gatgtaaaag cagcctttct tatttttgct gttttgagtt
     1141 tgatccttgg tactttattg ctgataagag tgcttaaaac aaatagaaat ctcagaaaat
     1201 aatctgaggt tttttaattt tcaataattt attagccctc acacgatcaa gattttcaag
     1261 gggataaaaa tttgggaaaa aattcacaaa aagacttgca tttttttgtg aaaagggtta
     1321 caataacaag tgaagaaaat cacaagagta tctttcttct taaactattt accaactatt
     1381 atttttagga ggaattgttg aatggcaaaa gaaacaaaaa cagaagcact cactgctgaa
     1441 gaagcagcta agcagtatac gaatagctta gttgaaaaag cgttaaaggc tgaaaaaatt
     1501 tatgcaactt atacacaaga acaggtagat aaaattgttg cagctatggc gcttgctggt
     1561 tctgaggcgt ccttgcagtt agctaaagaa gctcacgaag aaacaggccg tggggttgtc
     1621 gaagataaag atactaaaaa ccactttgcg acagaatatg tatatgaacg cataaaaaat
     1681 gaaaaaacgg ttggcatcat tggtgaagat aaagttgctg gcagtatcga aattgcagca
     1741 ccacttggtg tcttggcagg tatcgtacca acaactaacc caacatcaac gactatgttt
     1801 aaaatcttag tcgcattgaa aacacgcaat gcaatcgtct ttgctttcca cccagcagct
     1861 caaaaatgtt ctgctcatgc cgcccaaatc ttgtatgacg cggcggttaa agccggcgct
     1921 cctgaaaata ttgttcaatg gattgaaaca ccatccatcg caaacacggg agctttgatt
     1981 tccaacaaag gcattgcttc aatccttgca actggcggac ctggcatggt taatgcagcc
     2041 cttaaatctg gtaatccatc gatgggtgtt ggtgcaggta atggtgctgt ttatgtggat
     2101 gcaactgcta atcttgaacg tgcggttgaa gaccttcttc tttctaagcg ttttgacaat
     2161 ggtatgattt gtgcgactga aaattcggca gttgtggaag caccaattta taaagaatgg
     2221 cttgctaaaa tgcaggaaaa aggcgcttac cttgttccta aaaaagatta caaaaaattt
     2281 gaagattttg tctttaatga caaccatggt gttaacggac cggttgctgg tatgtcggcg
     2341 aaatggatct gtgaacaagc cggtgtgaca ttgccagaag gcaaagatgt tctcttattt
     2401 gaacttgata agaaaaaaat tggtgagaaa ttatcatcag aaaaactctc tccgcttctt
     2461 tctgtttata aagccaaaga tcgcaaagat ggtattgata ttgtagcagc tcttcttgat
     2521 tatcaaggtg ctggtcataa ctgtgccatc caaattggtt cacaagctga cccatttgtc
     2581 gctgaatatg gagatgccct caattcatct cgtgttttgg ttaaccaacc agactcagtc
     2641 ggtggtatcg gtgacgttta tacagacgca ttgcaagcca gcttaactct tggaacagga
     2701 tcatggggga aaaattcatt gtcacacaac ttatcgactg gcgacctctt gaatgtgaaa
     2761 actgttgcga aacgccgcaa ccgtcctcaa tgggttcgtt taccagaaaa aacttactac
     2821 gaaaaaaatg ctatttcata cttgcaggat gaaactgaaa caatgacgcg tgctatcatc
     2881 gttgccgatc caggtatgct tcaatttggt tttgttgaca ctgtccttgc tcagctatcc
     2941 cttcgggatg aaaaagtcgc aacttcaatt tatggtacta tcaaaccaga tccaacactc
     3001 ggtcagacta ttgaaattgc tagacaaatg cgtgacttca aaccagatac tgttattgca
     3061 gttggtggcg gttctgccct tgatgcagcc aaaattgcgc gttatatcta tgaatattcg
     3121 ttggatcaag aagcagattt ccttgatagt tatgaaaaag tcagtgagct cttccttgaa
     3181 ttgcagcaaa aattcattga tattcgtaag cggattgttc gcttcaagca tcagacagtg
     3241 actcgtctct tctgtatccc aacaacatct ggtacgggtt cagaagtgac accttatgcc
     3301 gttattactg atgatttcac acacgtgaaa tacccattga ctgattatga attgacaccg
     3361 caagttgcta ttgttgatcc agaatttgtc atgacagtgc ctaaacgtac cgtagctctt
     3421 tcaggacttg atactttgtc acatgctctt gaatcttacg tatctgttat ggcatctgat
     3481 ttcacacgtc catggtctat ggaagctatc agattggtta tcgaaaactt ggaagattct
     3541 tataactttg atcctaagaa tccaacactt cgtggagaac aagcacgtga aaacatgcat
     3601 tatgcagcaa ctttagcagg tatggccttc tcaaatgcct tccttggaat taaccactca
     3661 ctcgcccaca agactggcgg tgaatttgga cttcctcatg gtcttgctat ctcaattgct
     3721 atgcagcatg ttattcgcta caatggcgtt gcaggaaatg tgaaacgttc tgtttacccg
     3781 cgttatgaag aataccgtgc ccaacgtgat tatgcggata ttgctcgcgc tcttggactt
     3841 aaagggaaag atgatgcaga attggttgaa gcactctgtg cacgtattga tcaattaatg
     3901 catgctgtag atgttgagcc taagttatct gctaatgggg ttactaagaa ggcttttgat
     3961 gcagctgttg atagattggc aagcttggct tatgatgatc aatgtacacc agctaatcca
     4021 cgtcagcctt acattgaaga gatgaagcag ctcttgattg atcagttcta agttgatatc
     4081 gtttagcagt caggcccgct tatgcgggct ttttttgcct ctcgaaaacc actagctatg
     4141 ctagtagttc cggaaacttt tagcgtagta agaaaagaac ctcctaaaat gataagatag
     4201 aagtggtttc cccgccacta ctaaacatca caaaggggat agctcatgag agaggctaat
     4261 gaaagtttat cacatacaaa gtggaattgt tctaccatat tgttttcgca ctcaagtatc
     4321 atcgtcaaat catttatggc agatacaaaa ccagtatcgg aagaattatc tgggacttat
     4381 gtgaacgcaa aggcgtgacc attcatgaag cgaatgcctg ttccgatcat attcatatgc
     4441 ttgtcagtat cgcttccaaa ttcagtgttt cgtctttcat gggatattta aagggaaaga
     4501 gcagcttgat gatttttgat aagcatgcga acttaaaata caaatatggc aatcgaagtt
     4561 ttggtgtaga ggctattatg tagatacgat aggtcgtaac cagaaagtga tagctgaata
     4621 gatacaaaat caattacaag aagacagagt agcagaccag ctcactttat tcgagggagt
     4681 agatccgttt actgatgaaa tgaagaagaa gtaagtcgag gtgctttagc agctgctcgg
     4741 gaaagaggtg cgaggaagag ctatttcggt gggcctttgg ccctgaccgg tagaagtggc
     4801 ttacagtcgc agaacaaacc accagttcac actggtggtt ttgattgtta gcctatctta
     4861 ttttcccaaa gcttgtgtaa aacttctatt aaacagcaaa aagtaatctt ttccttagga
     4921 cacataatgt aaaattagct ggtaatgata gtttcagaac atcaaaaatg accgtttaag
     4981 acaaaatann nnnnnnnngg catttgaaca atttgatgta atggacagcc aaacactttc
     5041 aactgttgaa ggtgggaagg tatcgggtgg ggaagcagtc gcggcaattg gaggactggt
     5101 aggtgcgact cttgttactc catattgtgt aggcacttgg ggactcattc gatctcatta
     5161 aagaattaaa tggaatggat aattaatacc atggaattaa atgtaaataa ttataaaagt
     5221 ttaactaatg atgaattgtc agaagttttt ggtggagata aacaagctgc tgatacgttt
     5281 ctttcagcgg taggaggagc tgcatcagga ttcacctatt gtgcgtcaaa tggtgtgtgg
     5341 cacccatata ttcttgctgg atgtgctgga gttggtgcgg ttggatctgt agtttttcca
     5401 cactaaattg atataatggt gggcaatgtc aattcagggc gagatttctg aatgggtaca
     5461 gtggcattgt ccatttttcg tccaatagaa ttttttattt aagctgggct ataactatta
     5521 gaatattaag tattgactta tatactgtta aaaagattta tattatataa tgtcgaattt
     5581 caagagaggg aaaaaataag atgaagaaat tagagcgtat ctccacaaag attttgcgtt
     5641 gggcctattt aatcttaaca accatagttt ttgtagttgc tctgataata tataattggt
     5701 tatattatgg gaaatccatg cttttttggt taccagcagt tctggtatta ttagtaactg
     5761 ctttgttagc ctacttggta aaaaagcaat tggaagcgag gcttgagccc ttattaattt
     5821 tcaatacact tcagcaaatt attggtatat taatcgcctt agctgtaggt ctttttgcac
     5881 tggcatacaa agcattaaat actaaaatta atgatggtaa tgaggtctat tggatacttt
     5941 ttgctgccat cttactcatc ttttcttgga atgctgttga actctataaa gagctaaaaa
     6001 gaagaagcaa gaataagtaa agctaaggcc gaagcctaat agttgtctta aatattctgt
     6061 tattatcgta gttcttattg cttctactaa ttatggagga ggattaacta tgatagttaa
     6121 tttttatcaa tacaaagaac tgtctaatgt aaaattatca gccattaaga acatcggcaa
     6181 ccgcttgaaa agttgtcttt aaaagcgaat caattataat tacaagaaaa cgaaaggaaa
     6241 agttatttat gaatcgttac aactcattat tatcattttc agtgacagct ttgttgttag
     6301 tagcagtggt ggtacgtctc aagacttata taacaaggtg ttccaaccta cagatattat
     6361 gactaacaac tcctaaaatg tcacatctag ccaacaacct cacaacagcg gcagcgcaac
     6421 agaaacggtt gagcaatcaa ttctgcaggt acaggtgcga caccagatga tatctcaata
     6481 aagaccagtg atcaaccgca aacgaaagaa ggaatgaaac cagaaaaaga aaagttggat
     6541 actaatactt taatgaaagt tgtcaataaa gctttgtcac tttttgatag gctattgatt
     6601 aagttcggag cttaaaccat tactcgaaaa aatgcccatt ttgaacatct ttttggtagt
     6661 tgatcaactg tagtgggtga cgaaaaatta agtcctggag agaaccaatc tgtactgatc
     6721 cccaaaagtt gtactattta ttatttaaag gattggactt gtcaagaaaa agtagacagg
     6781 aagttaagtg aattagagag ggatggtttg attagtcgta gagattatca gcagatgcct
     6841 cctaaggtcg tctattctat aacagactat ggccgtactg ctaatacgat tattgatgtc
     6901 gtgtgccatt gcagagagga caatatcagg cggcgtcaaa tattaggcga agaaattgac
     6961 ttaatttcgt acgattttta agaagtgatt gttcatttat aaaaataaca tacattaagc
     7021 tatacttcct atacttcgat ttatatcaag gtctatgttc atatgtaacg cagaatttat
     7081 gactgcgaaa gtgcaacaat ttttaataga atattaactg atttgtacga tatagtaaaa
     7141 ttaaacatta ttccagtagc cgtttaaaaa ttctattatg ttatgtaata aatcatatca
     7201 gctaaatata gggtaatata ataccaaaaa aagaaaatca gttgacgtta attggctatt
     7261 ttgctaaaat aaaaactgtc gtagagacaa gaaacttttt cttggttcta ggattcgcca
     7321 aaacctagga ctcagattaa ggataaaaag gagaactatt atggcaatct caaaagagaa
     7381 aaaaaatgaa attattaaac aatacgcgcg tcatgacggt gacacaggtt cagtagaggt
     7441 acaagttgct gtccttactt gggagatcaa tcatcttaat gaacatatta aacagcataa
     7501 aaaagaccat gcaacttacc gtggattgat gaaaaagatc ggtcatcgcc gtaatctttt
     7561 agcttacctt cgccgtacgg acgttaatcg ttatcgtgat cttatcagct cacttggact
     7621 tcgtcgttaa ttgaagtgag caagcggctt acgatcttaa gcgtagcttg attaaataaa
     7681 aaagctgtcc attaggatgg cttttttagc tgtagtaggt caacatatag ttataggata
     7741 gcaaaagaaa attttaggaa taaaacacgt tttgaaacct ttacaaatgc tattgtagtt
     7801 attgttgtaa tagttttggt gttagaatca tcaaagcctg ttggttctat cgtgcgggct
     7861 gaaatggctt tttgaagttg tcataatatc gaaagagtat tggagtttaa ttataaggta
     7921 taaagtaaga taccaatgat tcgacactca tttataaacg ctaaaataga taataaagcc
     7981 cttgttaggt atctaatctt agtaagggct ttatagttga taagctcaat aagaaagcat
     8041 tgattatgat gaggactcaa atatcgctgg atgcctacct ctattcctta gaaattgagg
     8101 ttttctggta aatttagctt tttctagtct ttttcttttc cctaaaaata tgttaaaata
     8161 aacgagatac gtggttttgt gcaaagttaa gggagtaaga tagagctgta ctgttgtcag
     8221 ttatcgctgg gattgttgaa cttttgccta gcaatatcgt tgacgagaaa agcaatggga
     8281 tatggtgctg tatttttgat acattgacgt tagagtgata aggcttattt cttaaacttt
     8341 gtggaaaaca cgtagactaa tttttcagga gaaacttatg tcaaaacaag tgtttgaaac
     8401 aatttttgct ggcaaaaagc tggctgtaga aatcggtcag gttgccaaac aagccaatgg
     8461 tgctgctttg gttcgttatg gtgattcaac ggtcttgagt gctgcagtta tgtctaaaaa
     8521 aatgtccaca ggcgatttct tcccacttca gattaattat gaagaaaaaa gatacgcagc
     8581 tgggaaattc cccggtggct ttaacaagcg tgaaggccgg ccatcaaccg atgctacttt
     8641 aacggctcgc ttaattgatc gtccgattcg tcccatgttt gcggaaggtt ttcgcaatga
     8701 agtccaagtc attaatacag ttctgtctta tgatgctgat gcgagtgctc ctatggctgc
     8761 tatgtttggc agttcactag cactttcgat ttcagatatt ccttttaatg gcccgattgc
     8821 tggggtacaa gttgcttacc ttgatggtca atatgttatt aatcctacgg ctgaagaaaa
     8881 gaaagcttca cttttagaat tgactgtcgc cggaaccaag gaagccatca atatggttga
     8941 gtctggtgct aaggaattgt cagaagatat catgcttgaa gcccttctca aaggacatga
     9001 agcggtacgt gaactaattg ctttccaaga ggaaattata gcagctgttg gtaaagaaaa
     9061 agctgaagtt gaattgttac aggtggatgc tgatttgcag gctgaaattg ttggaaaata
     9121 caatgcagac cttcaaaagg ctgtccaaat tgaggaaaag aaagcccgcg aaattgcgac
     9181 agaagatgtc aaagagcacg tgactgctga atatgaagaa cgctatgctg aacacgagga
     9241 acatgaccat atcatgcgcg acgtagctga aatccttgaa cagatggaac acgcagaggt
     9301 tcgccgtctt atcacagaag acaaggttcg tcctgacggt cgtcgtgttg atgaaatccg
     9361 tccgcttgat gcagagattg atttcttgcc aaaagtacat ggttcaggtc tctttactcg
     9421 tggacaaacc caagccttgt cagtgttaac attggctcca atgggagata cccaaattgt
     9481 ggacgggctt gacgaagaat ataaaaaacg cttcatgcat cattataatt tcccgcaata
     9541 ttcggttggg gaaacgggac ggtatggagc gccaggacgc cgagaaatcg gtcatggagc
     9601 tcttggtgaa cgtgctttgg ctcaagttct gccaagtttg gaagcatttc cttatgccat
     9661 tcgtttggta gctgaagtgt tagaatcaaa cggttcctca tcacaggctt caatttgtgc
     9721 gggaacgctc gctcttatgg ctgggggtgt acctatcaaa gcaccagttg caggtattgc
     9781 tatgggactt atctcagacg gcactaacta taccgttttg accgatatcc aaggactaga
     9841 agatcacttc ggcgatatgg actttaaagt tgcaggtacc cgtgaaggta tcacagcttt
     9901 gcaaatggat attaagattg agggtattac accgcagatt ttagaggaag ctcttgccca
     9961 agctaaaaaa gcacgttttg aaattcttga tgtgatcgaa aaagtcattc ctgcaccacg
    10021 tctagaatta gcaccgacag cacctaaaat tgacacaatt aaagttgatg ttgataaaat
    10081 caagattgtc attggcaagg gcggtgaaac cattgataag attattgaag aaactggcgt
    10141 taagattgat attgatgaag acggtaatat tgctatctac tctagcgatc aagaggctat
    10201 caatcggact aaggaaatta ttgcaagttt ggtacgtgaa gctaaggttg gtgagatcta
    10261 tgaagctgaa gttgtccgta ttgagaaatt cggtgctttt gtgaatcttt ttgataagac
    10321 agatgcactc attcacatct ctgaaatagc ttggacccgc acgaataaag tagaagatgt
    10381 cttggctgtt ggagataagg ttacgg
//
