LOCUS       NZ_JAQCYD010000001     10231 bp    DNA     linear   CON 23-FEB-2024
DEFINITION  Streptococcus mutans strain AF36-21BH AF36-21BH.Scaf1, whole genome
            shotgun sequence.
ACCESSION   NZ_JAQCYD010000001
VERSION     NZ_JAQCYD010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus mutans
  ORGANISM  Streptococcus mutans
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605237)
  AUTHORS   Lin,X., Hu,T., Chen,J., Liang,H., Zhou,J., Wu,Z., Ye,C., Jin,X.,
            Xu,X., Zhang,W., Jing,X., Yang,T., Wang,J., Yang,H., Kristiansen,K.,
            Xiao,L. and Zou,Y.
  TITLE     The genomic landscape of reference genomes of cultivated human gut
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 605237)
  AUTHORS   Zou,Y., Lin,X. and Hu,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2022) BGI Reaserch, BGI-Shenzhen, Beishan
            Industrial Zone, Shenzhen 518083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: MGI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/23/2024 01:23:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,988
            CDSs (total)                      :: 1,943
            Genes (coding)                    :: 1,890
            CDSs (with protein)               :: 1,890
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 33 of 53
            Pseudo Genes (incomplete)         :: 28 of 53
            Pseudo Genes (internal stop)      :: 23 of 53
            Pseudo Genes (multiple problems)  :: 22 of 53
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:29:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 177386
            Orig. end                         :: 187617
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQCYD010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10231
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10231
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10231
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5231](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5231
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    186..200
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.66"
                     /tool="antismash"
     gene            575..871
                     /locus_tag="PGX70_RS00905"
     CDS             575..871
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /locus_tag="PGX70_RS00905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YlxR family protein"
                     /protein_id="WP_002263922.1"
                     /transl_table=11
                     /translation="MAKTRKIPLRKSLVSGEIIDKRDLLRIVKTKEGDVFIDPTGKKNG
                     RGAYIKLANEEALEAKKRKVFNRSFSMEIPESFYDELIAFVDHKVKRRELGLE"
     gene            864..1166
                     /locus_tag="PGX70_RS00910"
     CDS             864..1166
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262601.1"
                     /locus_tag="PGX70_RS00910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="WP_002262601.1"
                     /transl_table=11
                     /translation="MNNSKKLSNLLGLAQRAGRVISGEELVVKAIQTGQAQLIFLAKDA
                     GSNLTKKVTDKSNYYNIEVSTVFSALELSIAIGRNRKVLAIVDTGFSKKMRTFME"
     gene            1187..3937
                     /gene="infB"
                     /locus_tag="PGX70_RS00915"
     CDS             1187..3937
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003743 - translation initiation factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="infB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1190: GTP-binding protein LepA"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285798.1"
                     /locus_tag="PGX70_RS00915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="translation initiation factor IF-2"
                     /protein_id="WP_002296668.1"
                     /transl_table=11
                     /translation="MSKKRLYEIAKEIGKESKEIVEKAKSLGLEVKSHASSVEESDAKR
                     IVESFTVSVEPKAVTPTSKVEKEAKAQEGSVAAEPKAVATKPAGRPRPQNRNFKAEREA
                     RAKAEAERRQNNGERRNQNKGQNNRQKDNRNHGSQDRRNDNRNNRNRQNDNRRDNRNHF
                     QNRQEASKSQPTGPRFDFKARAAALKAEQNAEYSRQSETRFHEAQEAKRQAAQAKEKAK
                     KLNQKEQPTVEAAATAAPQAQPQTVEQVTHPAAVDTRRKKQARPDKSRDFSHENEDGPK
                     QNKHKKNRNKQNQVRNQKNSNWNKKNKKSKNNRNHNANLKPVTERKFHELPKEFEYTEG
                     MTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDADTIELLMVDYGIEAHQKVEVDTA
                     DIERFFVEDDYLNPKNMVERAPVVTIMGHVDHGKTTLLDTLRNSRIATGEAGGITQHIG
                     AYQIEEGGKKITFLDTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKA
                     ADVPIIVAINKIDKPGANPERVIGELAEYGVISTAWGGDSEFVEISAKFGQNIEELLET
                     VLLVAEIQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLHVQDPIVVGNTFGRV
                     RAMTNDLGRRVKVAAPSTPVSITGLNEAPMAGDHFAVYEDEKAARAAGEERAKRALLKQ
                     RQLTHRVSLDNLFDTLKAGEVKSVNVIIKADVQGSVEALAASLLKIDVEGVKVNVVHSA
                     VGAINESDITLAEASNAVIIGFNVRPTPQARQQAEADEVEIRLHSIIYKVIEEVEDAMK
                     GMLDPEFEEKIIGEALIRETFKVSKVGTIGGFMVTNGKITRDSSARVIRDGVVVFDGKL
                     ASLKHYKDDVKEVGNGQEGGLMIENYNDIKIDDTIEAYIMEEIKR"
     misc_feature    3945..3962
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.83"
                     /tool="antismash"
     gene            4171..4521
                     /gene="rbfA"
                     /locus_tag="PGX70_RS00920"
     CDS             4171..4521
                     /GO_function="GO:0019843 - rRNA binding [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rbfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262599.1"
                     /locus_tag="PGX70_RS00920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="WP_002262599.1"
                     /transl_table=11
                     /translation="MSNHRIERVGMEIKREVNEILQKKVRDPRVQGVTITDVQMSGDLS
                     LAKVYYTIMSDLASDNQKVQIGLEKAKGTIKRELGKNLTMYKIPDLTFIKDESIEYGNK
                     IDQMLRDLEKRN"
     gene            complement(4599..4733)
                     /locus_tag="PGX70_RS00925"
     CDS             complement(4599..4733)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318719.1"
                     /locus_tag="PGX70_RS00925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_002267544.1"
                     /transl_table=11
                     /translation="MEIEVDKLISSAQQPIVPIFKEYRQAYFENNRFLVDGKKPSLDL"
     gene            5001..5231
                     /locus_tag="PGX70_RS00930"
     CDS             5001..5231
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320866.1"
                     /locus_tag="PGX70_RS00930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_002275977.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.3e-16,
                     bitscore: 55.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTQAFEQFNVMDNEALSTVEGGGMIRCALGTAGSAGLGFVGGMG
                     AGTVTLPVVGTVSGAALGGWSGAAVGAATFC"
     misc_feature    5416..5430
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.87"
                     /tool="antismash"
     misc_feature    complement(5532..5545)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.34"
                     /tool="antismash"
     misc_feature    complement(5534..5550)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.54"
                     /tool="antismash"
     misc_feature    complement(5561..5576)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.41"
                     /tool="antismash"
     gene            5622..6065
                     /locus_tag="PGX70_RS00935"
     CDS             5622..6065
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264871.1"
                     /locus_tag="PGX70_RS00935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="WP_002267007.1"
                     /transl_table=11
                     /translation="MTTISNAEWEVMRVVWAKQMTSSSEIIAILSRTYCWSASTIKTLI
                     TRLSEKGYLTSQRQGRKYIYSSLISEEEALEQQVSEVFSRICVTKHQALIRHLIEETPM
                     TLSDIEKLEALLLSKKANAVPEVKCNCIVGQCSCHEHLEVTSK"
     gene            6062..8290
                     /locus_tag="PGX70_RS00940"
     CDS             6062..8290
                     /EC_number="7.2.2.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264872.1"
                     /locus_tag="PGX70_RS00940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_002267008.1"
                     /transl_table=11
                     /translation="MSEEVFLIDGMTCASCAINVENAVKKLDGIESAVVNLTTEKMTID
                     YDAAKVSEADVTKAVAGAGYGAKVYDPTTAESQEDREEHKLAGIKKRLLWTSIFTIPLF
                     YIAMGSMVGLPLPNFLAPSSAPLTYAMVLLLLTIPVIVLSWSFYDNGFRSLFKGHPNMD
                     SLVSLATTAAFLYSLYGTYHVYLGHTHHAHHLYYESVAVILTLITLGKYFETLSKGRTS
                     DAIKKLMHLSAKEATLIRDGEEIKVPIEQVQIGDKILVKPGEKIPVDGRVLSGHSAIDE
                     SMLTGESIPIEKMADSPVYAGSINGQGSLTFEAEKVGNETLLSQIIKLVENAQQTKAPI
                     AKIADKVSAVFVPVIITIAILTGLFWYFVMGQDFTFSMTISVAVLVIACPCALGLATPT
                     AIMVGTGRAAENGILYKRGDVLELAHQINTIVFDKTGTITQGKPEVVHQFSYHDRTDLV
                     QVTASLEALSEHPLSQAIVDYAKKEGTRLLAVEDFTSLTGLGLKGCVADETLLVGNEKL
                     MRQENISLEQAQADFKAATAQGQTPIFVASDGQLLGLITIADKVKNDSAATVKALQNMG
                     VEVAMLTGDNEETAQAIAKEVGITFVISQVLPQEKTQAILDLQAEGKKVAMVGDGINDA
                     PALATADIGISMGSGTDIAMESADIVLMKPAMLDIIKALKISRATIINIKENLFWAFIY
                     NVLSVPIAMGVLYLFGGPLLDPMIAGLAMSFSSVSVVLNALRLKVVKL"
     gene            8303..8506
                     /gene="copZ"
                     /locus_tag="PGX70_RS00945"
     CDS             8303..8506
                     /codon_start=1
                     /gene="copZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263706.1"
                     /locus_tag="PGX70_RS00945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="copper chaperone CopZ"
                     /protein_id="WP_002264873.1"
                     /transl_table=11
                     /translation="MEKTYHIDGLKCQGCADNVTKRFSELKKVNDVKVDLDKKEVTITG
                     NPSKWSLKRALKGTNYELGAEI"
     misc_feature    complement(8349..8361)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 22.29"
                     /tool="antismash"
     gene            8752..9573
                     /locus_tag="PGX70_RS00950"
     CDS             8752..9573
                     /EC_number="3.1.3.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1179: hydrolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351286.1"
                     /locus_tag="PGX70_RS00950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="WP_032506494.1"
                     /transl_table=11
                     /translation="MVKLIAIDMDGTLLNSRKEIPQENIAVIQEAARSGYKIVLCTGRM
                     QTGVEPYFEQLGLAAEQEYAILNNGCSLHTINRDWQLLTYHDLNFNGVSYLYDLLEGYP
                     EIDLTLTADRDYLVLADQVSELVAYDASLVFTQAQTVGLNDVKHAAKPVFQAMYLGESD
                     RLDAFQQKFECKLAEKFTTVRSQPYIFEVMPQSITKATGLKELAQKLRVSRTDIMAIGD
                     ALNDLEMLKAAGFSVAMGNASPEVKAAADLVTGSNDDAGVAQAIAKYVLNK"
     gene            complement(9679..10170)
                     /locus_tag="PGX70_RS00955"
     CDS             complement(9679..10170)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997911.1"
                     /locus_tag="PGX70_RS00955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YgjV family protein"
                     /protein_id="WP_018110171.1"
                     /transl_table=11
                     /translation="MLFSALGAFCLAYSSFTKEKQHMLLWQISDYIFTIIANLLLGGYT
                     GALTISVSIVRNALILKKKNTKVITICLVLIQIALGTYVNNLGLVGYLPIISSVSYTLT
                     TFLTSKMQFLRWLIIENMSLWFIYDLTINAYPTVCMDILIILSTLLALYKHRKKAGHSL
                     "
ORIGIN
        1 ctgaagtttt tgatggcact gttgaaatta tgagcgtggc acgtgaagct ggtgatcgaa
       61 cgaaggttgc ggttcgcagt cataatccta atgttgatgc tattggaact atagttggcc
      121 gcaaaggtgc caatattaag aaagtgacca gtaaattcca tcctaaacgt tttgatcaaa
      181 aaactggact ttgggttcca atggaagaga atgttgatgt tattgagtgg gttgatgatc
      241 cagcagaatt catttataat gctgttgctc ctgctgaggt tgatcaagtt ctctttgatg
      301 aagaagatga caaacatgca acagttgttg taccagacaa taagctgtca cttgctattg
      361 gccgtcgtgg tcaaaatgtc cgtttagccg ctcatttgac tggctatcgt attgatatta
      421 agtctgctag tgaatttgaa gaaatggaag ccgagtggac agcacaagat gaaacccaaa
      481 aggctactta tgaagaggct cctgctgagg aagtttctga actctctcaa gaatttgcag
      541 cagaagatgc aaaatagaaa gtcagaggtg tttgatggca aagacaagaa aaataccttt
      601 aagaaagtct cttgtttcag gagagattat tgataaacgt gatttgcttc ggattgttaa
      661 aaccaaggaa ggtgatgttt ttattgatcc tactggtaag aaaaatgggc gaggggctta
      721 tattaaactt gctaacgaag aagctttaga agctaaaaaa agaaaagtct ttaatcgcag
      781 tttttccatg gaaattcctg aaagctttta tgatgaatta attgcttttg ttgatcataa
      841 ggttaaaaga agagagttag gtcttgaata attcaaaaaa gttatcaaac ttattaggtt
      901 tggcccagag agcaggacgt gttatttcag gtgaagaatt agttgttaaa gctattcaaa
      961 ctggacaggc acagctcatc tttttagcca aagatgctgg ttctaatcta actaaaaaag
     1021 taacggataa aagtaactat tataatatag aagtctccac agtgttttca gcactggaat
     1081 taagtatcgc tattggcaga aacagaaaag ttcttgctat tgtggatact ggattttcaa
     1141 agaaaatgag gacctttatg gaataaaaag aataggagga caagatttgt cgaagaaaag
     1201 attatacgaa attgccaagg aaattggcaa agaaagtaaa gaaattgttg aaaaggcaaa
     1261 atcattgggc ctagaagtta aaagccatgc ttcaagcgtg gaagaaagtg atgctaaacg
     1321 tatagtagaa agttttactg tctctgtgga acccaaggct gttactccaa cttctaaagt
     1381 tgagaaagaa gccaaagcac aagaaggatc tgttgcagcg gaacctaaag cagttgctac
     1441 aaagcctgca gggcgtccaa gaccgcaaaa tcgtaatttt aaagcagaac gagaagcgcg
     1501 tgccaaagca gaggctgaac gtcgtcaaaa taatggtgaa cgtcgaaacc aaaataaagg
     1561 acagaacaat cggcaaaaag acaatcgtaa tcatggttcc caagatcgtc gtaatgataa
     1621 ccgcaataat cgcaatcgtc aaaatgacaa tcgtcgcgat aatcgtaatc attttcaaaa
     1681 tcgacaagaa gcatcaaaat cccagccgac aggaccgcgt tttgatttca aagcacgtgc
     1741 tgcagcctta aaagcagaac aaaatgcgga atattcgcgt cagagtgaaa cacgttttca
     1801 tgaagctcaa gaagctaaac gtcaagccgc tcaagctaaa gaaaaagcta agaagctaaa
     1861 tcagaaagag cagccgacgg ttgaagctgc agcaactgca gcgccacaag ctcagcctca
     1921 gacagttgaa caagtgacac atccagcagc tgtggataca cgtcgcaaaa aacaggctcg
     1981 cccagataaa tcacgtgatt tttcacatga gaatgaagac ggaccaaaac aaaataaaca
     2041 taagaaaaat cggaataaac aaaaccaagt gagaaatcaa aaaaatagta attggaataa
     2101 gaaaaataaa aaatctaaaa ataaccgtaa tcataatgcc aatctgaaac ctgtgactga
     2161 acgcaaattc catgaactac caaaggaatt tgagtataca gaaggtatga cggttgctga
     2221 aattgcaaaa cgaattaagc gtgagcctgc cgaaattgta aaaaaactct ttatgatggg
     2281 agttatggca actcaaaacc aatctttgga tgctgatacg attgaactcc taatggttga
     2341 ttatggcatt gaagcacatc aaaaagtgga agttgataca gctgatattg aacgtttctt
     2401 cgttgaagat gattatctta atccgaaaaa tatggttgaa cgtgcgcccg ttgtaacgat
     2461 tatggggcat gttgaccatg gtaagacaac attgcttgat acattgcgta attcccgtat
     2521 agcgacaggt gaagctggag gaattaccca acatattggg gcctatcaaa ttgaagaagg
     2581 tggaaagaaa attactttcc ttgatacacc cggacatgca gccttcacaa gtatgcgggc
     2641 acgcggcgct tcggtaactg atattaccat tcttattgta gctgctgatg atggtgttat
     2701 gccacaaacg attgaagcta ttaaccactc caaagctgct gatgttccaa ttattgttgc
     2761 tattaataaa attgataagc cgggtgctaa tccagaacgt gtcatcggtg agttagctga
     2821 gtatggcgtt atttcaactg cttggggcgg cgattctgaa tttgttgaaa tttcagctaa
     2881 attcggtcaa aatattgaag aacttttaga aactgttctt ctagtagcag aaatccaaga
     2941 actgaaagct gacccaactg ttcgtgctat tggtacagtt atcgaagctc gccttgataa
     3001 aggaaaaggt gcagtagcga cgcttttagt acaacaagga acgctgcatg ttcaagatcc
     3061 aatcgttgtt gggaacacat ttggacgtgt gcgtgctatg actaatgacc ttggacgtcg
     3121 tgttaaggtt gctgctccat caacgccagt ctctatcact ggtttgaatg aagccccaat
     3181 ggcaggtgac cattttgccg tttatgagga tgaaaaggct gcgcgtgctg ctggggaaga
     3241 aagagctaaa cgtgccctgc tgaaacaacg tcaattgact catcgtgtca gtcttgacaa
     3301 tctctttgat acgctcaaag ccggtgaggt taaatctgtt aatgttatta tcaaagcaga
     3361 tgtgcaaggt tccgttgaag ctcttgcagc ctctcttttg aagattgacg ttgaaggtgt
     3421 gaaagttaat gtggtccact cagctgttgg tgccatcaat gaatcagata tcaccttggc
     3481 agaagcttct aatgctgtta tcattggttt caatgttcgc ccaactccac aggcccgtca
     3541 gcaggccgaa gcagatgaag ttgaaattcg tttgcattcc attatttata aggtaattga
     3601 agaagtagaa gatgccatga aaggtatgct tgatccagag tttgaagaaa aaattattgg
     3661 tgaagcgctt attcgtgaga ccttcaaagt ttctaaagtg ggaactattg gcggcttcat
     3721 ggtaacaaat ggtaaaatta cgcgtgattc aagtgctcgt gttattcgtg atggtgtggt
     3781 tgtctttgac ggtaagctcg ctagtttgaa acattataaa gatgatgtta aagaggttgg
     3841 taatggtcaa gaaggcggtc tcatgattga aaattacaat gacatcaaaa ttgatgatac
     3901 tattgaggct tacattatgg aagaaattaa aagataataa tatttagtta ctcctttgtt
     3961 aagacttcta ggcatacgca agtacagtcg ttgtcgtgtt gccttagatg aacgtcatat
     4021 gatttctttt gaaattcaaa gataaacaat aaagctaggt catctggcct agcttttttc
     4081 agaagatgag cgaatgaagt gagcttttac aagatatttt ttgtcgtggt gaaaaaagaa
     4141 aattgtaaaa ttaaaagaaa ggaaaagaga atgtctaatc atcgtattga acgtgttggt
     4201 atggaaatta agcgcgaagt taatgagatt ttgcaaaaga aggtacgtga cccacgtgtt
     4261 caaggtgtca ctattactga tgtccaaatg tcaggtgatt tatctctggc aaaggtttac
     4321 tatactatca tgagtgacct tgcttctgat aatcagaaag ttcaaatagg tcttgaaaag
     4381 gctaaaggga ccattaaacg tgaacttgga aaaaatttga ccatgtataa aattcccgat
     4441 ttaactttta tcaaagatga atctattgag tatggtaata agattgacca aatgctgcgg
     4501 gatttagaaa aaagaaatta agtaaacaaa aaagtttctt ctgtctgctt gttaattgct
     4561 gtaaaagtgc gaaaataggt gtcaagcctt cgttaggatt ataagtccaa tgaaggcttt
     4621 tttccatcaa ccaaaaaacg attattttcg aagtaggcct gtctatactc tttaaaaatc
     4681 ggaacaatag gttgttgtgc tgacgatatc agcttatcta cttctatttc catctcagaa
     4741 ggcctcccaa gccttttgac ttttttgagc ttgtcgtgct ccagtatgtt tgtagtcagt
     4801 tgcgatgcga gtaggcgaaa actattttta atttttattg actattatta caggcattat
     4861 aacgtcattt ttaaccgttt aggacaaaat agctaccgtt taggatattt tgctccattt
     4921 tgaaaaataa tttgctatat tagagatgtc ggttggctaa ccagacaaaa aattaaaaat
     4981 ggaaaagagg tatctatcat atgaatacac aagcatttga acaatttaac gtaatggata
     5041 atgaagcact ttcaactgtt gagggtggtg gtatgattag atgtgcactt ggcacagctg
     5101 gttctgcagg tttaggattt gtagggggta tgggagctgg tacagttact ctcccagtcg
     5161 ttggtacagt atctggagcg gctttaggag gctggtctgg agcagctgta ggtgctgcta
     5221 ctttttgttg aacatgaaaa atagaagatc cttattgatc ggaagtctgc ctttattatt
     5281 cttgttattt atggcagtaa aaaatctttt taaggagctc tcgccaatca tttatagcat
     5341 taatttacta ggtctgttag gtttgttagt cttatggttt aaaattaaca agaatcacaa
     5401 gagctaatga ggactgtatc attttgaaaa attattattg aagaactgtt cattgtttga
     5461 gtgaagaggc agaaactaag gtttctcgcc tctttttttg ctcttttaaa aagctgataa
     5521 gtagggtcat attttgtcaa aaaattaaaa atcagttttt gttgacaaat gtagacaaag
     5581 aggttataat atatctacaa atgtagatga aaggagctca aatgacaact atttcaaatg
     5641 cggaatggga agtaatgcgt gttgtctggg ctaagcagat gactagcagc agtgaaatta
     5701 ttgctatctt aagtcgtact tattgctggt ccgcttcaac gattaaaacg ctgatcacgc
     5761 gtttatcaga aaaaggctac ttgaccagtc agcgtcaagg aagaaaatac atctattcta
     5821 gtttgatttc agaagaagag gcattagagc agcaagtgtc ggaagttttc tcgcgcattt
     5881 gtgtcacgaa gcatcaagct ttgattagac acttaattga ggaaacgcct atgactttgt
     5941 ctgatattga aaaattggaa gctctgctat tgtccaaaaa agcaaatgct gtgcctgaag
     6001 ttaagtgtaa ttgtattgtc gggcaatgtt cttgccatga acatttggag gtgacatcaa
     6061 aatgagtgaa gaagtatttt tgatagatgg catgacctgt gcctcttgtg ccatcaatgt
     6121 tgaaaatgcc gttaaaaaac tagacggtat tgaaagtgca gtagtcaatt tgacaactga
     6181 aaaaatgacg atagattacg atgccgctaa ggttagtgaa gcagatgtta ctaaggcagt
     6241 tgctggtgca ggctatggcg ctaaagttta tgacccaacg acggctgaaa gtcaggagga
     6301 tcgtgaagaa cataagttag caggtattaa aaaacgtctt ttgtggactt ctatttttac
     6361 cattcccctc ttttatattg ctatgggaag tatggttggc ttgcccttac ctaacttttt
     6421 agcaccaagc agtgctccgc tcacttatgc gatggttttg cttcttttga caattccggt
     6481 tatcgtgtta agttggagct tctatgacaa tggttttaga tcgcttttta aaggtcatcc
     6541 taatatggac tcattagtgt ccttagcaac aacagcggcg tttctttaca gtctttatgg
     6601 aacttaccat gtttacttgg gacatacaca tcatgctcac catctctatt atgaatcggt
     6661 agctgttatt ttaactctca ttactttagg gaaatacttt gaaaccctat cgaaaggtcg
     6721 aacctcagat gcgataaaaa aattaatgca tttgtctgct aaagaggcta ctctgatacg
     6781 tgatggcgag gagattaagg ttcctattga gcaagtgcaa atcggagata aaattttagt
     6841 caaacccggt gaaaaaatac ctgtggatgg tcgagtcctg tcagggcatt ctgctattga
     6901 tgaatccatg ttaacagggg aatctattcc tattgaaaaa atggcggata gccctgttta
     6961 tgccgggtca atcaatggtc agggaagtct gacttttgag gctgaaaaag ttggcaatga
     7021 aaccttgctt tcacaaatta ttaaattggt tgagaacgct cagcaaacta aggcacccat
     7081 tgccaagatt gctgataagg tatctgctgt ctttgtacct gttattataa cgattgctat
     7141 tttgactggt ctcttctggt attttgtcat gggacaagac tttacctttt caatgacaat
     7201 cagtgtcgct gttcttgtca ttgcctgccc ttgtgctttg ggtcttgcaa cgccaacggc
     7261 tattatggtt ggtacaggtc gtgctgctga aaatggaatc ctttataaac gtggtgatgt
     7321 cttggaattg gcacatcaga ttaatacgat tgttttcgat aaaacaggca ctattactca
     7381 aggtaaacca gaagttgttc atcaattttc ttatcatgat cgaactgatt tagtgcaagt
     7441 gacagcttcc ttagaagcat tatctgaaca tccccttagt caggccattg ttgattatgc
     7501 taaaaaagaa gggactcgtt tacttgcagt ggaagacttt acttctctaa cagggctagg
     7561 actgaaaggt tgtgttgctg atgaaacttt gcttgttggt aatgaaaaat tgatgcgtca
     7621 agaaaatatc tctctagaac aggctcaagc agattttaag gcagcaacag ctcagggaca
     7681 aacacccatt tttgttgcca gtgatggtca attgttagga ctgattacga ttgcggacaa
     7741 ggtaaagaat gacagtgcag caacagttaa agctttacag aatatggggg ttgaagtggc
     7801 catgctgacg ggtgataatg aagaaactgc gcaggctatt gccaaagaag tggggattac
     7861 ttttgttatc agtcaagttt tgccgcagga aaaaacacag gccattcttg atttgcaggc
     7921 ggaaggcaag aaagttgcca tggttgggga cggtattaat gacgctccgg cacttgcgac
     7981 agcagatatt ggcatttcca tgggctctgg aacagatatt gccatggagt ccgcggatat
     8041 tgtcttgatg aaacctgcaa tgcttgatat tattaaggca ctgaaaatta gtcgtgctac
     8101 tattattaat attaaagaaa atcttttctg ggcatttatt tataatgttc tgtcagttcc
     8161 tattgctatg ggagtacttt atctctttgg cggacctttg ctggatccaa tgattgctgg
     8221 tctagccatg agctttagct ctgtttctgt tgttctaaat gccctgcgtc ttaaagttgt
     8281 aaaattataa aggagaatta tcatggaaaa aacatatcat attgatggct taaaatgcca
     8341 aggctgcgct gacaatgtca ccaaacgctt ttcagaatta aagaaagtca atgatgtcaa
     8401 agttgacctt gataaaaaag aagtcacgat tacaggaaat ccaagcaagt ggtctcttaa
     8461 acgagcactg aaaggaacca attatgaatt gggagcagaa atttaagtag gctccatata
     8521 ccttaaaaac tgtaaagaca cagttatttc attgcatctt atcagatggt cacaaagagg
     8581 gaaaagactg gaaaaccagt ttttttctgc tgtaaaatga atttatagca ttgtcatagt
     8641 tatctgtttc aattattgta gaatatacag aattataata acttgctgtg ccagtttcct
     8701 tgatttgtga tacactagat agtggaatgt tttagaaaag gttatatgat tatggttaaa
     8761 ctgattgcta ttgatatgga tggcacgctt ttgaatagtc gaaaagaaat cccgcaagaa
     8821 aatattgctg ttattcaaga agcagctcgt tcgggctata aaattgttct ttgcacaggc
     8881 cgtatgcaga ctggtgttga gccctacttt gaacaattag gtttggctgc tgaacaagaa
     8941 tatgcgattc taaataatgg ctgctctctt cacactatta atagagattg gcagttactg
     9001 acttatcatg atttaaactt taatggagta tcttatttgt atgatttgct tgagggttat
     9061 cctgaaatcg atttaacatt gactgctgat agggattatc tggttttggc tgatcaagta
     9121 tcagaattag tagcttatga tgcgagtctg gtatttacgc aagcacagac tgtgggctta
     9181 aatgatgtaa agcatgctgc taaaccagtt tttcaggcta tgtatttagg agagagcgat
     9241 cgcttagatg ctttccagca aaaatttgag tgcaaattag cagaaaaatt tactactgta
     9301 agaagtcagc cttacatttt tgaagtgatg cctcaaagca ttacaaaagc tactggtttg
     9361 aaagaattag ctcagaaatt aagagtttca aggactgata ttatggcaat aggagatgca
     9421 cttaatgatc ttgaaatgct caaagcagca ggtttcagtg tggctatggg aaatgctagt
     9481 ccagaagtta aggctgcagc agaccttgtg actggttcta atgatgatgc tggtgttgct
     9541 caagctattg ccaagtatgt cttaaataag tgataaatca tgaaaaagga actgggaaat
     9601 cgttttcatc tgcttaacac tagaaaatag aaagttcagg atcaattaaa taagaatagg
     9661 aagcaggaca aagccaattc acaaggaatg tcctgctttt tttcgatgtt tatataaagc
     9721 cagcagtgtt gagagaatga tcaaaatatc catgcaaaca gtagggtagg cgttgatagt
     9781 gaggtcataa atgaaccaga gagacatgtt ttcaataatc aaccagcgca aaaattgcat
     9841 tttggatgtt aaaaaggttg tcaaagtgta agagacagaa gaaataatgg gcaagtaacc
     9901 gactaagccc aagttattca cataagtacc taaagcaatt tgaatgagga ctaaacagat
     9961 ggtaataact tttgtattct ttttcttaag aataagtgca tttctgacga tggaaacact
    10021 aatggttagg gcgcctgtat agccacccag taaaagatta gcaataatcg taaaaatata
    10081 gtcgctgatt tgccaaagta gcatgtgctg tttctcttta gtaaaagaag aataagcgag
    10141 gcaaaaagca ccgagggctg aaaaaagcat tgcaattgga agaattcatt aaagttacca
    10201 tttttgattg tcaaaaatta atactcttca a
//
