LOCUS       NZ_JAQEMJ010000050      2832 bp    DNA     linear   CON 22-FEB-2024
DEFINITION  Bacillus velezensis strain TM115-48 GF3HH28002B.Scaf50, whole genome
            shotgun sequence.
ACCESSION   NZ_JAQEMJ010000050
VERSION     NZ_JAQEMJ010000050.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus velezensis
  ORGANISM  Bacillus velezensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Lin,X., Hu,T., Chen,J., Liang,H., Zhou,J., Wu,Z., Ye,C., Jin,X.,
            Xu,X., Zhang,W., Jing,X., Yang,T., Wang,J., Yang,H., Kristiansen,K.,
            Xiao,L. and Zou,Y.
  TITLE     The genomic landscape of reference genomes of cultivated human gut
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Zou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2022) BGI Reaserch, BGI-Shenzhen, Beishan
            Industrial Zone, Shenzhen 518083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: MGI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/22/2024 04:06:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,263
            CDSs (with protein)               :: 4,263
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 69 of 133
            Pseudo Genes (incomplete)         :: 78 of 133
            Pseudo Genes (internal stop)      :: 16 of 133
            Pseudo Genes (multiple problems)  :: 26 of 133
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:15:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2832
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQEMJ010000050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2832
                     /collection_date="2014-02-01"
                     /country="China: Shenzhen"
                     /db_xref="taxon:492670"
                     /host="Homo sapiens"
                     /isolation_source="fecal material [ENVO:00002003]"
                     /lat_lon="22.32 N 114.05 E"
                     /mol_type="genomic DNA"
                     /organism="Bacillus velezensis"
                     /strain="TM115-48"
                     /submitter_seqid="GF3HH28002B.Scaf50"
     region          1..2832
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..2832
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2832
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[162:2306](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      163..2306
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(109..120)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.59"
                     /tool="antismash"
     gene            163..522
                     /locus_tag="PGW53_RS22125"
     CDS             163..522
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004403393.1"
                     /locus_tag="PGW53_RS22125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_224443548.1"
                     /sec_met_domain="PF13471 (E-value: 3.3e-24, bitscore: 73.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGFNKTFNRYTFKYKKMKKTAFNEERYQEIFKQIEELFYMLDVVC
                     AWYPRKADCIHKTFLGYKFLRRKYSIPVDMVVGVRKFPFEAHAWLQVNNQNFFDDSEET
                     SRYNIILSSKNLSKG"
     gene            519..2306
                     /locus_tag="PGW53_RS22130"
     CDS             519..2306
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870873.1"
                     /locus_tag="PGW53_RS22130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_224443549.1"
                     /sec_met_domain="GATase_7 (E-value: 8e-19, bitscore: 55.6,
                     seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 1.4e-30, bitscore:
                     94.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIKWFLASFSSDNNVGRQIEIQKAIFNQYKISTKLNSASIHNC
                     KFYIASNKELESICTFKNVILIGDICIHNKEELLEKLNIMKPFNISDEKLICILYREKG
                     IKLINDLVGTFSFILYDKNNDKVYAVRDQLGIKTLFWTKQKNETVIASNIFLLNDQSLR
                     RDLNKVYFKEFYQRNGIIDSELTPYKHVFRLSSGSFLEIRNGKERVERYWDLTNIKDNL
                     RYSNETDYFEHFVGLLKISTENRMKKGKNNSIMLSGGLDSTSIYALAKMSEDNKQKITP
                     VSAVFDELKECDERDLISGLLKKYNDEAIYLNFDNLLMFERFPNGIPFSDEPSVNCISF
                     DFTYQIVKKSVQYGFDNILSGYAGDHLLTGSLHATRDMIEKGQLKKAFSYLTNYSIATN
                     TSAFQNFIEFTLFPNIPKHFISDTNSEYYSQLTNKFKKINNKNQQEFYYQISNAKSHLY
                     TDRVIGALTGSDIHHPFLDRRLVEFMYNVPGEIRFSDVNPKYILRRSMESYLTSDITSK
                     INKTTHIAYTYKSIRANWDKIYDFMKDAILAEELKLISVDYWREELDRWRNGVKVSDYF
                     WTLFSIELWLKQYYNLIKK"
     gene            2358..2579
                     /locus_tag="PGW53_RS22135"
     CDS             2358..2579
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847606.1"
                     /locus_tag="PGW53_RS22135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_065180754.1"
                     /transl_table=11
                     /translation="MQNYIKPSLIDCGKSESLIKGSCGWGSENWTLDKTGAYHTTRRKM
                     VSVGWLPGFTEVFKCKSVKACSSDKNLC"
ORIGIN
        1 aatataaaat agacaaaaaa actgtaaaaa gagatattct tgattttatt tatggcttag
       61 caagagcagg aattgtgaaa gtttatgaat agaaacaacc aattcatatc gttcatttac
      121 ttattaactc acttgatatt atttgactta gaattattta ttatgggatt taataaaaca
      181 ttcaatcgtt atactttcaa atataaaaaa atgaaaaaaa cagcatttaa tgaggaaaga
      241 tatcaggaga tatttaaaca aattgaagag ttattttata tgctcgatgt ggtttgtgca
      301 tggtatccaa gaaaagccga ctgtattcat aaaacgtttc taggatataa atttcttaga
      361 agaaaatatt ctatccctgt tgatatggta gtaggtgtac gaaaatttcc atttgaagca
      421 catgcttggc tacaagttaa taatcaaaat ttctttgatg atagtgagga gacttcccga
      481 tataacatta ttttatcttc gaaaaattta agtaaagggt gattagtatt aagtggtttt
      541 tagcttcgtt ttcatctgat aacaacgtag ggagacaaat cgaaattcaa aaagctattt
      601 tcaaccaata taaaatttca acaaaattga atagtgcttc tatacataac tgtaagtttt
      661 acattgcttc aaacaaagaa ttagaatcaa tttgcacctt taaaaatgta atattaatag
      721 gcgatatttg tatacataac aaggaagaat tattagaaaa actaaacatt atgaaaccct
      781 ttaatatttc agatgagaaa ttgatatgta tcttgtaccg agaaaaaggt attaaattaa
      841 ttaatgatct agtaggaact ttttcattta ttctctacga taagaacaac gataaagttt
      901 atgccgttag agatcaacta ggaataaaaa cattattttg gacaaagcaa aaaaatgaaa
      961 ctgttattgc aagtaatatt ttcttactta acgatcagag tttaagaaga gaccttaata
     1021 aagtatattt caaagagttt tatcaaagaa acgggattat cgactctgaa ctgacaccat
     1081 ataagcatgt gttcagatta tcaagcggca gttttcttga aatacgaaat gggaaagaac
     1141 gagtagaacg ttattgggac ttaacaaata tcaaggataa tttaagatac tcaaatgaaa
     1201 ctgattattt cgaacatttc gtcgggttat taaaaatttc tacagaaaat agaatgaaaa
     1261 aaggaaaaaa taattccata atgcttagcg gcggcctgga ttcgacctca atttatgcac
     1321 tggctaaaat gtcggaggac aataagcaaa aaataacacc tgtaagtgct gtgtttgatg
     1381 agttaaagga atgtgatgaa agagatctta ttagtgggtt attaaaaaaa tataatgacg
     1441 aagctatata tctaaatttt gacaatttgt taatgtttga gcgtttccct aatggaatcc
     1501 cattttcaga tgaaccaagt gtaaattgta tttcatttga tttcacatat caaattgtca
     1561 aaaagtctgt acaatatggt tttgacaata ttttaagtgg ctatgcggga gatcacttat
     1621 taactggatc tttgcacgca acacgagaca tgatagagaa aggacaacta aaaaaagcct
     1681 tctcttattt aacaaattac tctatagcta caaatacatc agcttttcaa aatttcattg
     1741 agtttacttt atttccaaat atacctaagc attttatctc agatacaaac agtgaatact
     1801 attcgcaact tacaaataag ttcaaaaaaa tcaataataa aaaccagcag gaattttatt
     1861 atcaaataag taatgcaaaa tctcatttgt atacagatcg tgtaattgga gcgttgactg
     1921 gttcagatat acatcatcca ttcttggata gaagactagt agaatttatg tacaatgttc
     1981 caggcgagat cagattttct gatgtaaatc caaaatacat tttaagaagg tcaatggaaa
     2041 gttatcttac tagtgatatc acatcaaaga taaataaaac aacccacatt gcttatacgt
     2101 ataaaagcat acgcgccaat tgggataaaa tctatgattt tatgaaagat gcaattttag
     2161 ctgaagagtt aaaattgatt tctgtagatt attggagaga ggaacttgac agatggagaa
     2221 atggagtgaa agtaagtgat tacttttgga cattattctc tatcgaactt tggcttaaac
     2281 aatactataa tttgataaaa aagtaattag tataagaata ttgtgttatt taatagggat
     2341 cgataggaga gaatgatatg caaaattaca tcaagccttc attaatagat tgtgggaaaa
     2401 gtgaaagtct aattaaagga tcatgcggtt ggggcagtga aaactggact ttagataaaa
     2461 caggtgctta ccatactaca agacgaaaaa tggtaagtgt tggttggctt ccgggtttta
     2521 ctgaagtatt taaatgtaaa tctgtaaaag catgcagttc tgataaaaat ctttgctaaa
     2581 tacaattaaa taaaaactag ttgacaatta ataaaaaata aatgtatccc tattaaatac
     2641 acaatattgt tatatatgca ttacatgaaa atctataata gatctaatta cttaatgaaa
     2701 atggcgtggg tgaaataata tgaataatct tctttgggta atttatatat taattttttg
     2761 taccacactt tggtacttta aggtgaagat ttcaaaaaat attcggttca taaagaaaag
     2821 aataaatgca cc
//
