LOCUS       NZ_JAQEMJ010000032      8978 bp    DNA     linear   CON 22-FEB-2024
DEFINITION  Bacillus velezensis strain TM115-48 GF3HH28002B.Scaf32, whole genome
            shotgun sequence.
ACCESSION   NZ_JAQEMJ010000032
VERSION     NZ_JAQEMJ010000032.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus velezensis
  ORGANISM  Bacillus velezensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Lin,X., Hu,T., Chen,J., Liang,H., Zhou,J., Wu,Z., Ye,C., Jin,X.,
            Xu,X., Zhang,W., Jing,X., Yang,T., Wang,J., Yang,H., Kristiansen,K.,
            Xiao,L. and Zou,Y.
  TITLE     The genomic landscape of reference genomes of cultivated human gut
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Zou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2022) BGI Reaserch, BGI-Shenzhen, Beishan
            Industrial Zone, Shenzhen 518083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: MGI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/22/2024 04:06:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,263
            CDSs (with protein)               :: 4,263
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 69 of 133
            Pseudo Genes (incomplete)         :: 78 of 133
            Pseudo Genes (internal stop)      :: 16 of 133
            Pseudo Genes (multiple problems)  :: 26 of 133
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:15:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8978
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQEMJ010000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..8978
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:7625](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..7625)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..4430)
                     /gene="srfAB"
                     /locus_tag="PGW53_RS21535"
                     /pseudo=""
     CDS             complement(<1..4430)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (0-272). E-value:
                     7.7e-98. Score: 319.1. Matches aSDomain:
                     nrpspksdomains_PGW53_RS21535_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (437-839). E-value: 7e-116.
                     Score: 379.1. Matches aSDomain:
                     nrpspksdomains_PGW53_RS21535_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (945-1014). E-value: 8.5e-23. Score:
                     72.4. Matches aSDomain: nrpspksdomains_PGW53_RS21535_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1029-1317). E-value:
                     1e-104. Score: 341.7. Matches aSDomain:
                     nrpspksdomains_PGW53_RS21535_Condensation_LCL.2"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="PGW53_RS21535"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="AMP-binding (E-value: 2.2e-125, bitscore:
                     406.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.7e-16, bitscore:
                     47.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 9.7e-96, bitscore:
                     308.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYILNQLGQASTSYNVPAVLLLEGSVDKNRLEEAMQALINRHETL
                     RTSFDMADGEVVQTIHKNVSFELETAEGREEDAEELTKAFIRPFALNHAPLVRSKLIRL
                     EEDRHLLLIDMHHIITDGSSMGIFIGDLAKLYQGTELELPKIHYKDFSVWQREKANLDQ
                     HEAYWLDTFKGDLPVLDLPLDFPRPAERSFEGERVIFGLDKQVTAQIKKLLADTDTTMY
                     MFLLAAFQVLLSKYSGQEDIIVGSPAAGRQHPDLQDVPGMFVNTVALRSHPAGKKTFKQ
                     FLDEVKTASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVLFNMQNMEIPSLRLGDLE
                     ISSYSMHHHVAKFDLSLEAAERGEEVGLSFDYAKALFADETIRRWSAHFVNLIKACAEN
                     PDIQLSDASLLSAPEREALLSDEKRTEADLPEGTFVSLFERQAQKTPDLTAVAGGTSLT
                     YRELDERSNRFARHLQACGTGSEDIVAIMMDRSADLIPAILGVMKAGAAFLPIDPETPE
                     ERIRYSLEDSGTKLLVVNERNMTAAAVYKGKTVVMEDGEWRNESADRLETEPGADQLAY
                     IIYTSGTTGKPKGVQLEHRNLINYVTWFSREAGLTEADKSVLLSSYAFDLGYTAIFPIL
                     QAGGELHIVPKETYTAPDQLGEYIQKNGITYMKLTPSLFHMIVNTARFTSERRFSPLRL
                     VVLGGEKIITSDVRKFHDVYAHTDFINHYGPTETTIGAIAGRINMERLDQYEQRPVIGR
                     PIANTGALVLDGAMQLVPPGASGELYITGKGLARGYLHRPQLTAEKFLSNPFSPDSLMY
                     KTGDVVRRLSDGTIEFIGRADDQVKIRGYRIELKEVETVLLSVNGIQEAVVLAVSEGGL
                     PELCAYYKAGSELKGSELRKRLSETLPSHMLPAYFVQVDRIPLTANGKTDKNALPKPGV
                     SQTAQTASALPETELEEKLCRIWKQTLGTDTLGIDDNFFDYGGHSLKGMMLLANIQAEL
                     DKTVPLKALFEQPTVRLLAVYIEKSAVSEGYRMISPAASADAYPLSSAQKRMYVLNQLD
                     RETISYNMPSVLLMEGEVNISRLQEALNQMINRHESLRTSFIDQNGRPMQQIAEQADID
                     LHIFEAADEEKADLIIQAFIKPFDLSAAPLIRAALVRLNEKKHLLLLDMHHIIADGVSR
                     SMLVKELAHLYKGGSLPSPNLHYKDFAVWQNEPEQAERMKDHERYWLSAFSGELPELNL
                     PTDFPRPPVQSFKGQSVRFRAGRETEKAVRELMESSGATLHMVLHAAFHVFLSKITGQR
                     DIIIGSVTAGRTSAEVQEMPGMFVNTLALRNETRKEQTFAGLLEQVKQTNLDALAHQDY
                     PFEDLIGKLDLPRDMSRNPLFQVMVTTEDPDKETLELENLRITPYESNQGTAKFDLTLG
                     GFTDQEGLGLQFEYAADLFKKETIEKWSAGFLRILKQAAESPDRKLPEISLISDAEKQA
                     LLDEWKGKTISVPRD"
     source          1..8978
                     /collection_date="2014-02-01"
                     /country="China: Shenzhen"
                     /db_xref="taxon:492670"
                     /host="Homo sapiens"
                     /isolation_source="fecal material [ENVO:00002003]"
                     /lat_lon="22.32 N 114.05 E"
                     /mol_type="genomic DNA"
                     /organism="Bacillus velezensis"
                     /strain="TM115-48"
                     /submitter_seqid="GF3HH28002B.Scaf32"
     region          1..8978
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..8978
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(327..440)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0014"
                     /evalue="5.40E-19"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="1368"
                     /protein_start="1330"
                     /score="60.6"
                     /tool="antismash"
                     /translation="KQTNLDALAHQDYPFEDLIGKLDLPRDMSRNPLFQVMV"
     aSDomain        complement(480..1343)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21535_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.00E-104"
                     /label="PGW53_RS21535_Condensation_LCL.2"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="1317"
                     /protein_start="1029"
                     /score="341.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="DAYPLSSAQKRMYVLNQLDRETISYNMPSVLLMEGEVNISRLQEA
                     LNQMINRHESLRTSFIDQNGRPMQQIAEQADIDLHIFEAADEEKADLIIQAFIKPFDLS
                     AAPLIRAALVRLNEKKHLLLLDMHHIIADGVSRSMLVKELAHLYKGGSLPSPNLHYKDF
                     AVWQNEPEQAERMKDHERYWLSAFSGELPELNLPTDFPRPPVQSFKGQSVRFRAGRETE
                     KAVRELMESSGATLHMVLHAAFHVFLSKITGQRDIIIGSVTAGRTSAEVQEMPGMFVNT
                     LALRNET"
     CDS_motif       complement(489..578)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0013"
                     /evalue="1.20E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="1314"
                     /protein_start="1284"
                     /score="43.4"
                     /tool="antismash"
                     /translation="DIIIGSVTAGRTSAEVQEMPGMFVNTLALR"
     CDS_motif       complement(837..869)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0012"
                     /evalue="1.90E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="1198"
                     /protein_start="1187"
                     /score="14.1"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       complement(900..965)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0011"
                     /evalue="6.60E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="1177"
                     /protein_start="1155"
                     /score="28.3"
                     /tool="antismash"
                     /translation="MHHIIADGVSRSMLVKELAHLY"
     CDS_motif       complement(1164..1274)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0010"
                     /evalue="7.50E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="1089"
                     /protein_start="1052"
                     /score="50.5"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGEVNISRLQEALNQMINRHESLRTSF"
     aSDomain        complement(1389..1595)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21535_PCP.1"
                     /evalue="8.50E-23"
                     /label="PGW53_RS21535_PCP.1"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="1014"
                     /protein_start="945"
                     /score="72.4"
                     /tool="antismash"
                     /translation="EEKLCRIWKQTLGTDTLGIDDNFFDYGGHSLKGMMLLANIQAELD
                     KTVPLKALFEQPTVRLLAVYIEKS"
     aSModule        complement(1389..4430)
                     /complete
                     /domains="nrpspksdomains_PGW53_RS21535_Condensation_LCL.1"
                     /domains="nrpspksdomains_PGW53_RS21535_AMP-binding.1"
                     /domains="nrpspksdomains_PGW53_RS21535_PCP.1"
                     /locus_tags="PGW53_RS21535"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1884..1949)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0009"
                     /evalue="1.30E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="849"
                     /protein_start="827"
                     /score="36.5"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIELKEVE"
     aSDomain        complement(1914..3119)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21535_AMP-binding.1"
                     /evalue="7.00E-116"
                     /label="PGW53_RS21535_AMP-binding.1"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="839"
                     /protein_start="437"
                     /score="379.1"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FERQAQKTPDLTAVAGGTSLTYRELDERSNRFARHLQACGTGSED
                     IVAIMMDRSADLIPAILGVMKAGAAFLPIDPETPEERIRYSLEDSGTKLLVVNERNMTA
                     AAVYKGKTVVMEDGEWRNESADRLETEPGADQLAYIIYTSGTTGKPKGVQLEHRNLINY
                     VTWFSREAGLTEADKSVLLSSYAFDLGYTAIFPILQAGGELHIVPKETYTAPDQLGEYI
                     QKNGITYMKLTPSLFHMIVNTARFTSERRFSPLRLVVLGGEKIITSDVRKFHDVYAHTD
                     FINHYGPTETTIGAIAGRINMERLDQYEQRPVIGRPIANTGALVLDGAMQLVPPGASGE
                     LYITGKGLARGYLHRPQLTAEKFLSNPFSPDSLMYKTGDVVRRLSDGTIEFIGRADDQV
                     KIR"
     CDS_motif       complement(2031..2120)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0008"
                     /evalue="6.20E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="800"
                     /protein_start="770"
                     /score="50.5"
                     /tool="antismash"
                     /translation="PPGASGELYITGKGLARGYLHRPQLTAEKF"
     CDS_motif       complement(2649..2708)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0007"
                     /evalue="4.40E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="594"
                     /protein_start="574"
                     /score="31.4"
                     /tool="antismash"
                     /translation="AYIIYTSGTTGKPKGVQLEH"
     CDS_motif       complement(2895..2936)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0006"
                     /evalue="5.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="512"
                     /protein_start="498"
                     /score="16.5"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     CDS_motif       complement(3453..3566)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0005"
                     /evalue="3.20E-15"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="326"
                     /protein_start="288"
                     /score="48.4"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVLFNM"
     aSDomain        complement(3615..4430)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21535_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="7.70E-98"
                     /label="PGW53_RS21535_Condensation_LCL.1"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="272"
                     /protein_start="0"
                     /score="319.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="MYILNQLGQASTSYNVPAVLLLEGSVDKNRLEEAMQALINRHETL
                     RTSFDMADGEVVQTIHKNVSFELETAEGREEDAEELTKAFIRPFALNHAPLVRSKLIRL
                     EEDRHLLLIDMHHIITDGSSMGIFIGDLAKLYQGTELELPKIHYKDFSVWQREKANLDQ
                     HEAYWLDTFKGDLPVLDLPLDFPRPAERSFEGERVIFGLDKQVTAQIKKLLADTDTTMY
                     MFLLAAFQVLLSKYSGQEDIIVGSPAAGRQHPDLQDVPGMFVNTVALRSH"
     CDS_motif       complement(3621..3710)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0004"
                     /evalue="9.70E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="270"
                     /protein_start="240"
                     /score="46.9"
                     /tool="antismash"
                     /translation="DIIVGSPAAGRQHPDLQDVPGMFVNTVALR"
     CDS_motif       complement(3957..3992)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0003"
                     /evalue="2.00E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="158"
                     /protein_start="146"
                     /score="14.0"
                     /tool="antismash"
                     /translation="HYKDFSVWQREK"
     CDS_motif       complement(4023..4088)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0002"
                     /evalue="1.30E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="136"
                     /protein_start="114"
                     /score="30.5"
                     /tool="antismash"
                     /translation="MHHIITDGSSMGIFIGDLAKLY"
     CDS_motif       complement(4284..4397)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21535_0001"
                     /evalue="3.50E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="PGW53_RS21535"
                     /protein_end="49"
                     /protein_start="11"
                     /score="51.5"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGSVDKNRLEEAMQALINRHETLRTSF"
     gene            complement(4352..4516)
                     /gene="comS"
                     /locus_tag="PGW53_RS21540"
     CDS             complement(4352..4516)
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: protein motif:HMM:NF036673.4"
                     /locus_tag="PGW53_RS21540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_269195014.1"
                     /transl_table=11
                     /translation="MRARRNSSLNRSDKRLIRSIILFPQHSAGCISLISSDRPARATTS
                     LLYFCWKDQ"
     gene            complement(<4584..7625)
                     /gene="srfAB"
                     /locus_tag="PGW53_RS21545"
                     /pseudo=""
     CDS             complement(<4584..7625)
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-29). E-value: 2.4e-15.
                     Score: 47.5. Matches aSDomain:
                     nrpspksdomains_PGW53_RS21545_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-306). E-value:
                     1.2e-112. Score: 367.8. Matches aSDomain:
                     nrpspksdomains_PGW53_RS21545_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-864). E-value:
                     5.9e-112. Score: 366.2. Matches aSDomain:
                     nrpspksdomains_PGW53_RS21545_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1013). E-value: 1.4e-14. Score:
                     46.0. Matches aSDomain: nrpspksdomains_PGW53_RS21545_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="PGW53_RS21545"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.6e-91, bitscore:
                     294.5, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.6e-120, bitscore:
                     390.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.1e-09, bitscore:
                     25.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKSIQKVYALTPMQEGMLYHALLDPHSSSYFTQLELRIHGSFQ
                     LELFEKSVNELIRTYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISQLDEARQTEYI
                     ERYKRGVQQQGFHLAKDILFKAAVFRLSEKELYLVWSNHHIVMDGWSMGVLMKSLFQNY
                     EALRAGRPAGGSQGKPYSDYIKWLGGRDYEEAEQYWSSRLADFEQPSLLPGRLAAEKKD
                     YQNEEYSFVWDEELVAQIQQTANRHQVTGPNLFQAVWGAVLSKYNYTDDVVFGTVVSGR
                     PSEINGIETMAGLFINTIPVRIKIDKDAAFSDVMAAVQKNAVEAERYDYVPLYDIQKRS
                     ALDGSLLNHLVAFENYPLDKELENGGMEERLGFSIKVEHAFEQTSFDFNLIVYPGKTWT
                     VKIKYNGAAFAHDAIERTAHHLTRMMKAAVGTPDAPVRELGLVSGKEERQIVEVFNDTK
                     TALPEEEAVHRLFEAQANRTPASSAIKEAGREWTYREVNEAANRLARRLVKSGLEKGRT
                     AAIMNDRSAETVIGMLAVLKAGGAYVPIDPAFPEDRLRFMAEDSSIRLVLTVQAYQEQA
                     GALQVPVVMLDESADETVSGTDLNLPAGGNDLAYIMYTSGSTGKPKGVMIEHRNIIRLV
                     KHSNYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGASLHPVKKETLLDAGQFAQFLKE
                     QRITTMWLTSPLFNQLAQKDAGMFNTLRHLIIGGDALVPHIVSKVRKASPELSLWNGYG
                     PTENTTFSTSFLIDQDYDGSIPIGKPIGNSTAYIMDENRNLQPIGAPGELCVGGSGVAR
                     GYVNLPELTEKQFVRDPFRPDEMIYRTGDLAKWLPDGTIEFLGRIDNQVKVRGFRIELG
                     EIEAKISQAENVTESAAVIRKNKADENEICAYFTADQALLPEDLRKTLSESLPEYMIPA
                     HFIQMNQFPLTANGKIDKKALPEPQAEAVQKEYEAPKTEAEQKLADIWEGILGVKAGVT
                     DNFFTIGGHSLKAMMMTAKIQEHFQ"
     aSDomain        complement(4587..4715)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21545_PCP.1"
                     /evalue="1.40E-14"
                     /label="PGW53_RS21545_PCP.1"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="1013"
                     /protein_start="970"
                     /score="46.0"
                     /tool="antismash"
                     /translation="EQKLADIWEGILGVKAGVTDNFFTIGGHSLKAMMMTAKIQEHF"
     aSModule        complement(4587..7607)
                     /complete
                     /domains="nrpspksdomains_PGW53_RS21545_Condensation_DCL.1"
                     /domains="nrpspksdomains_PGW53_RS21545_AMP-binding.1"
                     /domains="nrpspksdomains_PGW53_RS21545_PCP.1"
                     /locus_tags="PGW53_RS21545"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(5004..5069)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0009"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        complement(5034..6215)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21545_AMP-binding.1"
                     /evalue="5.90E-112"
                     /label="PGW53_RS21545_AMP-binding.1"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="864"
                     /protein_start="470"
                     /score="366.2"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEAQANRTPASSAIKEAGREWTYREVNEAANRLARRLVKSGLEKG
                     RTAAIMNDRSAETVIGMLAVLKAGGAYVPIDPAFPEDRLRFMAEDSSIRLVLTVQAYQE
                     QAGALQVPVVMLDESADETVSGTDLNLPAGGNDLAYIMYTSGSTGKPKGVMIEHRNIIR
                     LVKHSNYVPVHEEDRMAQTGAVSFDAGTFEVFGALLNGASLHPVKKETLLDAGQFAQFL
                     KEQRITTMWLTSPLFNQLAQKDAGMFNTLRHLIIGGDALVPHIVSKVRKASPELSLWNG
                     YGPTENTTFSTSFLIDQDYDGSIPIGKPIGNSTAYIMDENRNLQPIGAPGELCVGGSGV
                     ARGYVNLPELTEKQFVRDPFRPDEMIYRTGDLAKWLPDGTIEFLGRIDNQVKVR"
     CDS_motif       complement(5151..5240)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0008"
                     /evalue="2.20E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="825"
                     /protein_start="795"
                     /score="48.7"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGSGVARGYVNLPELTEKQF"
     CDS_motif       complement(5742..5801)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0007"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="628"
                     /protein_start="608"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       complement(5988..6029)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0006"
                     /evalue="2.40E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="546"
                     /protein_start="532"
                     /score="20.5"
                     /tool="antismash"
                     /translation="LAVLKAGGAYVPID"
     CDS_motif       complement(6555..6671)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0005"
                     /evalue="2.20E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="357"
                     /protein_start="318"
                     /score="55.5"
                     /tool="antismash"
                     /translation="QKNAVEAERYDYVPLYDIQKRSALDGSLLNHLVAFENYP"
     aSDomain        complement(6708..7607)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21545_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="1.20E-112"
                     /label="PGW53_RS21545_Condensation_DCL.1"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="306"
                     /protein_start="6"
                     /score="367.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVMDG"
                     /tool="antismash"
                     /translation="QKVYALTPMQEGMLYHALLDPHSSSYFTQLELRIHGSFQLELFEK
                     SVNELIRTYDILRTVFVHQQLQKPRQVVLAERKTKVHYEDISQLDEARQTEYIERYKRG
                     VQQQGFHLAKDILFKAAVFRLSEKELYLVWSNHHIVMDGWSMGVLMKSLFQNYEALRAG
                     RPAGGSQGKPYSDYIKWLGGRDYEEAEQYWSSRLADFEQPSLLPGRLAAEKKDYQNEEY
                     SFVWDEELVAQIQQTANRHQVTGPNLFQAVWGAVLSKYNYTDDVVFGTVVSGRPSEING
                     IETMAGLFINTIPVRIKID"
     CDS_motif       complement(6720..6815)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0004"
                     /evalue="7.30E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       complement(7137..7202)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0003"
                     /evalue="3.50E-12"
                     /label="C3_DCL_135-156"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="163"
                     /protein_start="141"
                     /score="38.6"
                     /tool="antismash"
                     /translation="NHHIVMDGWSMGVLMKSLFQNY"
     CDS_motif       complement(7419..7535)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0002"
                     /evalue="2.30E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="69"
                     /protein_start="30"
                     /score="45.6"
                     /tool="antismash"
                     /translation="SYFTQLELRIHGSFQLELFEKSVNELIRTYDILRTVFVH"
     aSDomain        complement(7539..7625)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21545_NRPS-COM_Nterm.1"
                     /evalue="2.40E-15"
                     /label="PGW53_RS21545_NRPS-COM_Nterm.1"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="29"
                     /protein_start="0"
                     /score="47.5"
                     /tool="antismash"
                     /translation="MSKKSIQKVYALTPMQEGMLYHALLDPHS"
     CDS_motif       complement(7560..7598)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21545_0001"
                     /evalue="2.30E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="PGW53_RS21545"
                     /protein_end="22"
                     /protein_start="9"
                     /score="17.0"
                     /tool="antismash"
                     /translation="YALTPMQEGMLYH"
     gene            complement(7647..>8978)
                     /locus_tag="PGW53_RS21550"
     CDS             complement(7647..>8978)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (0-280). E-value:
                     1.5e-156. Score: 514.2. Matches aSDomain:
                     nrpspksdomains_PGW53_RS21550_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (285-439). E-value: 7.3e-54.
                     Score: 173.7. Matches aSDomain:
                     nrpspksdomains_PGW53_RS21550_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057766256.1"
                     /locus_tag="PGW53_RS21550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_270354896.1"
                     /sec_met_domain="Condensation (E-value: 5e-80, bitscore:
                     256.8, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DKHHWNQSVMLHAKDGFDPEITEKTLHVLTVHHDALRMIYREQKP
                     YYRGLEDVSVELNVFELNGPAEDHEDRIEREADRLQSSISLETGHLLKAGLFRAEDGDH
                     LLLAIHHLVVDGVSWRILLEDFTSVYTQLKQGNEPALPPKTHSFAEFAERIKEYANTKA
                     FLKEADYWRELEEKEVCTQLPKDRQSGDQRMKHTRTVSFSLTPEQTEQLTTNVHEAYHT
                     EMNDILLTALGLALKEWTGEDTIGVHLEGHGREDILDGLNITRTVGWFTSMYPMILEMK
                     HADDLSYQLKQMKEDIRHIPNKGVGYGILRYVTAPEHKENLSFEIDPDISFNYLGQFNE
                     MSDSGLFTRSGMPSGQSLSPDTEKPNALDIVGFIENGQMTMTFAYHSLEFHEKTIQSFS
                     DSFKGHLLKIIDHCLAQDGPELTPSDLGDDDLTLDELDKLMEIL"
     aSDomain        complement(7659..8120)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21550_TIGR01720.1"
                     /evalue="7.30E-54"
                     /label="PGW53_RS21550_TIGR01720.1"
                     /locus_tag="PGW53_RS21550"
                     /protein_end="439"
                     /protein_start="285"
                     /score="173.7"
                     /tool="antismash"
                     /translation="LSYQLKQMKEDIRHIPNKGVGYGILRYVTAPEHKENLSFEIDPDI
                     SFNYLGQFNEMSDSGLFTRSGMPSGQSLSPDTEKPNALDIVGFIENGQMTMTFAYHSLE
                     FHEKTIQSFSDSFKGHLLKIIDHCLAQDGPELTPSDLGDDDLTLDELDKL"
     aSModule        complement(7659..8975)
                     /domains="nrpspksdomains_PGW53_RS21550_Epimerization.1"
                     /domains="nrpspksdomains_PGW53_RS21550_TIGR01720.1"
                     /incomplete
                     /locus_tags="PGW53_RS21550"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(7965..7994)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21550_0004"
                     /evalue="3.00E-03"
                     /label="NRPS-E7"
                     /locus_tag="PGW53_RS21550"
                     /protein_end="337"
                     /protein_start="327"
                     /score="10.1"
                     /tool="antismash"
                     /translation="PDISFNYLGQ"
     CDS_motif       complement(8040..8096)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21550_0003"
                     /evalue="3.10E-09"
                     /label="NRPS-E6"
                     /locus_tag="PGW53_RS21550"
                     /protein_end="312"
                     /protein_start="293"
                     /score="29.1"
                     /tool="antismash"
                     /translation="KEDIRHIPNKGVGYGILRY"
     aSDomain        complement(8136..8975)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_PGW53_RS21550_Epimerization.1"
                     /evalue="1.50E-156"
                     /label="PGW53_RS21550_Epimerization.1"
                     /locus_tag="PGW53_RS21550"
                     /protein_end="280"
                     /protein_start="0"
                     /score="514.2"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="DKHHWNQSVMLHAKDGFDPEITEKTLHVLTVHHDALRMIYREQKP
                     YYRGLEDVSVELNVFELNGPAEDHEDRIEREADRLQSSISLETGHLLKAGLFRAEDGDH
                     LLLAIHHLVVDGVSWRILLEDFTSVYTQLKQGNEPALPPKTHSFAEFAERIKEYANTKA
                     FLKEADYWRELEEKEVCTQLPKDRQSGDQRMKHTRTVSFSLTPEQTEQLTTNVHEAYHT
                     EMNDILLTALGLALKEWTGEDTIGVHLEGHGREDILDGLNITRTVGWFTSMYPMILEM"
     CDS_motif       complement(8142..8189)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21550_0002"
                     /evalue="1.30E-08"
                     /label="NRPS-E5"
                     /locus_tag="PGW53_RS21550"
                     /protein_end="278"
                     /protein_start="262"
                     /score="26.8"
                     /tool="antismash"
                     /translation="ITRTVGWFTSMYPMIL"
     CDS_motif       complement(8598..8663)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_PGW53_RS21550_0001"
                     /evalue="1.40E-12"
                     /label="NRPS-E2"
                     /locus_tag="PGW53_RS21550"
                     /protein_end="126"
                     /protein_start="104"
                     /score="39.6"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
ORIGIN
        1 ttgtcccgcg gcacagaaat tgtttttcct ttccattcgt caaggagggc ttgtttttca
       61 gcatcgctga tcagtgaaat ctcaggcagc tttctgtccg gactttctgc cgcttgcttc
      121 agaatccgca ggaacccggc gctccacttt tcaattgttt cttttttaaa cagatcagcc
      181 gcatattcaa actgaaggcc gaggccttct tgatccgtaa agccgccgag tgtcagatcg
      241 aatttcgccg tgccttggtt cgactcatac ggagtgatgc gcaggttctc cagttccagt
      301 gtttctttat ccggatcttc tgtcgtcacc atgacttgga acaacggatt tcggctcata
      361 tctctcggca ggtcaagctt tccgatcaga tcctcaaacg gataatcctg atgcgccagc
      421 gcgtcgagat tggtttgttt cacctgttcg agcagcccgg cgaaggtttg ctctttgcgc
      481 gtttcattgc gaagcgcaag cgtattgaca aacatccccg gcatttcctg aacttcggcg
      541 ctcgttcttc cggcggtcac cgagccgata atgatatcgc gctgccccgt gattttgctc
      601 aaaaagacat ggaacgcggc gtgaagcacc atatgaagag tcgctcccga tgattccatt
      661 aattcacgca ccgctttttc cgtttcacgc cccgctctga aacggacgga ttgtccttta
      721 aaactttgaa ccggcggacg ggggaaatcg gtcggcagat tcaattcagg aagctcgccg
      781 gaaaatgcgg agagccaata acgctcatgg tctttcatcc gttcggcctg ctcaggttca
      841 ttctgccaga cggcgaagtc tttatagtgc aggttcggcg acggcaggct tccgcctttg
      901 taaaggtgag cgagctcttt gaccagcatg cttctggaca cgccgtcggc aataatatga
      961 tgcatatcca gcagcagcag gtgtttcttt tcattcagtc tgacaagagc ggcccgaatg
     1021 agcggagccg cgctcagatc aaacggctta atgaatgcct gaatgataag atccgctttc
     1081 tcctcgtctg ctgcttcaaa gatgtgcagg tcgatgtcag cctgttctgc gatctgctgc
     1141 atcgggcggc cgttttgatc gataaaggac gtccgcaaag actcgtggcg attgatcatc
     1201 tgattgagcg cttcctgaag ccttgaaata ttgacttctc cttccatcag aagaacggac
     1261 ggcatattat agctgattgt ctcccggtca agctggttca ggacgtacat ccgtttttgg
     1321 gcggatgata acgggtacgc gtctgcactg gctgccggtg agatcatccg atatccctca
     1381 gataccgctg atttttcgat atatacggca agcaggcgga cagtcggctg ttcgaacagc
     1441 gcttttaacg gaaccgtttt gtccagttca gcctgaatat tggcgaggag catcatgcct
     1501 tttaaagagt gcccgccgta atcgaagaaa ttatcgtcga tgccgagcgt atccgtaccg
     1561 agtgtctgtt tccaaatgcg gcacagtttt tcctccaatt ccgtttccgg caaggctgaa
     1621 gctgtttgcg ccgtttggct gacgcccggt ttcgggaggg cgtttttatc ggtttttccg
     1681 ttggctgtga gcggaatgcg gtctacttgc acgaaatagg ccgggagcat atgagacggc
     1741 agtgtttcgg aaagccgttt gcgaagctca gagcctttca gctcgctgcc ggctttatag
     1801 tacgcgcaca gttccggcag cccgccttcg ctgactgcga gaacgaccgc ctcctgaatg
     1861 ccgtttacgc ttaacagcac ggtttcaacc tcttttaatt caatgcggta gccccggatt
     1921 ttcacctgat catccgcacg gccgataaat tcaatcgtcc cgtcagaaag cctgcggacg
     1981 acatctcccg ttttgtacat caggctgtcc ggtgaaaaag gatttgagag aaatttctcc
     2041 gccgtcagct gcggacggtg aagatatcct ctggcaagcc cctttccggt aatataaagc
     2101 tcgccgcttg cgcccggagg cacgagctgc attgctccgt ccaatacaag cgcgccggta
     2161 ttcgcgatcg ggcggccgat gacgggacgc tgctcatatt gatcaaggcg ctccatattg
     2221 atccgtccgg caatggcgcc gatcgtcgtt tccgtcggcc cgtagtgatt gatgaagtcg
     2281 gtatgggcgt atacgtcatg aaacttgcgg acatcggacg tgatgatctt ttctccgccg
     2341 agcaccacta aacgaagcgg gctgaaacgg cgttctgacg taaaacgggc tgtattgacg
     2401 atcatatgga acaatgacgg cgtcaatttc atatacgtaa tgccgttttt ctgtatatac
     2461 tcaccaagct gatcaggcgc ggtgtacgtc tctttcggca caatgtgcag ctctccgccc
     2521 gcctgaagaa tcgggaatat agccgtatag ccgagatcaa atgcgtaaga agacagcagc
     2581 acggatttgt cagcttcggt caaaccggct tcacggctga accatgtgac ataattgatt
     2641 agattgcggt gctccagctg gacgcctttc ggtttgcccg ttgttcccga agtataaatg
     2701 atatatgcaa gctggtcggc gccgggctcc gtttcaagcc ggtcggcgct ttcgttccgc
     2761 cattctccgt cttccattac gaccgttttt cctttataaa ctgcggccgc ggtcatgttt
     2821 ctctcattga cgaccagaag cttcgttccg ctgtcttcaa gagagtagcg gattctctct
     2881 tcaggagtct ccggatcaat cgggagaaat gccgctccgg ctttcatgac gccgagaatc
     2941 gcgggaatca agtcggccga acgatccatc ataatggcca cgatgtcctc actgcccgtt
     3001 ccgcaagcct gaaggtggcg ggcaaaccgg ttcgagcgtt catcaagctc acgatatgtc
     3061 agacttgtgc cgcccgcaac cgccgtgaga tcaggcgttt tttgcgcttg ccgttcaaac
     3121 agcgaaacaa aagtgccctc aggcagatcc gcttccgtcc gtttttcatc agaaaggagc
     3181 gcttcccgct caggagcgga cagcaggctt gcgtcggaca gctgaatatc cggattttca
     3241 gcgcaggctt taatcagatt gacaaaatga gcgctccagc ggcggatggt ttcgtccgca
     3301 aataaggctt tcgcgtaatc aaagctcaat ccgacctctt ctccgcgttc agccgcttct
     3361 aaggaaagat caaatttagc gacatgatga tgcatggagt aagaagaaat ctccaaatct
     3421 ccgagccgca gggacgggat ctccatgttc tgcatattaa ataacacgct gaacagcgga
     3481 ctccggcttg tgtccctcgt taacggcagt ttttcaatta attcttcaag cggatagctt
     3541 tgatgctcaa aagcttgaag gctcgccgtt ttcacttcat ccagaaattg cttgaacgtt
     3601 tttttgccgg caggatgcga ccgaagcgcc acagtgttga caaacatgcc cggcacgtct
     3661 tggagatcag gatgctgtct tccggccgcc ggagacccga ctatgatgtc ttcctgcccc
     3721 gaatatttgg acagcagcac ttgaaaagcg gctaacaaga acatgtacat cgtcgtatcc
     3781 gtatcagcca gcagcttctt aatctgcgcc gtcacctgtt tatcaagccc gaagatgacg
     3841 cgttcccctt caaaactgcg ctcggcagga cgcgggaaat caagcggcag atccagcacc
     3901 ggcagatcgc ctttgaacgt atcaagccag taagcttcgt gctgatcgag atttgctttt
     3961 tcacgctgcc agactgaaaa atccttataa tgaatctttg gcagctcaag ctccgtgcct
     4021 tgataaagct tcgcaagatc accgatgaaa atgcccattg agcttccgtc cgtaataatg
     4081 tggtgcatgt caatcagcag aagatgccga tcttcttcaa gccggatcag cttcgaacgg
     4141 accagcggcg catgattgag cgcaaacggc ctgataaagg cttttgtcag ctcttctgca
     4201 tcttcttccc ggccctcggc ggtttcaagc tcgaacgaca catttttatg aatggtctgc
     4261 acgacttctc catctgccat gtcaaacgac gtacgcagtg tctcatgacg gttgattaat
     4321 gcctgcatgg cctcttcaag acggtttttg tctactgatc cttccagcag aagtacagca
     4381 gggacgttgt agctcgtgct ggcctgtccg agctgattaa ggatatacat cctgcgctgt
     4441 gctgaggaaa caggataatg cttctgataa ggcgcttgtc ggatcggttc aaactgctgt
     4501 tcctcctcgc tctcatgctc caaataatac gcaagctcct gaatggtcgg cttttcaaat
     4561 aacactttaa tcggcacttc tttttgaaag tgttcctgga ttttagcggt catcatcata
     4621 gcttttaaag aatgtccgcc aatcgtgaag aaattgtcag tcacacccgc ttttacaccg
     4681 agaatgcctt cccaaatgtc cgcgagtttc tgctccgctt ccgttttcgg cgcttcgtat
     4741 tctttttgaa cggcttcagc ctgaggctca ggcagcgctt ttttatcaat ttttccgttc
     4801 gccgtcagcg gaaactgatt catctggatg aagtgcgcgg gaatcatgta ttccggaagt
     4861 gattccgaaa gcgttttgcg caggtcttcc ggcaaaaggg cttggtctgc ggtaaagtaa
     4921 gcgcagattt cattttcatc cgctttattt ttccggatca cagccgcaga ttccgtcaca
     4981 ttctccgcct ggctgatttt cgcctcgatt tcgccgagct cgattctgaa accgcggacc
     5041 tttacttgat tgtcaattct gccgagaaac tcgatcgtgc cgtccggaag ccacttcgcc
     5101 aagtcccccg tccggtatat catttcatcc ggtctgaacg gatcgcggac aaactgcttc
     5161 tccgttagtt caggcagatt cacatagcct cttgccactc cgcttccgcc gacgcacagc
     5221 tcaccgggag cgccgatcgg ctgaaggttg cggttttcgt ccataatgta cgcagtggaa
     5281 tttccgatcg gcttcccgat cgggatcgag ccgtcgtagt cctgatcaat gagaaaactc
     5341 gtcgaaaacg tcgtattctc cgtcggcccg tagccgttcc aaagcgacag ctccggtgat
     5401 gccttcctca ctttgctgac gatatgcggc acaagcgcat caccgccgat gatgaggtgc
     5461 cggagcgtgt taaacatgcc ggcatccttt tgggcaagct gattaaacag cggagacgtc
     5521 agccacatgg tcgtgatccg ctgctctttc agaaattggg cgaattgtcc ggcgtcaagc
     5581 agcgtctctt ttttcaccgg gtgcaatgac gctccgttca gcaatgcgcc gaagacttcg
     5641 aaggttccgg catcaaagct gacggctccc gtttgcgcca tccggtcttc ttcatgaacc
     5701 ggcacgtaat tcgaatgttt gacgagcctg atgatatttc tgtgttcaat catgacgcct
     5761 ttcggtttgc cggtcgatcc ggatgtatac atgatatacg ccaagtcgtt gccgccggcc
     5821 ggaagattca agtctgttcc gcttaccgtt tcatccgcgc tttcatccag catgacgacc
     5881 gggacttgca atgcgcccgc ttgttcttga tatgcctgaa ccgtcagcac gagccgaatc
     5941 gagctgtctt ccgccataaa gcggagacgg tcctccggaa aagccggatc aatcggcaca
     6001 tatgcgccgc ctgcttttag gacggcaagc attcctataa cggtttccgc agaccggtcg
     6061 ttcataatag cggcggtccg gcctttttcc agcccgctct tcacaaggcg tctcgccagg
     6121 cggttggccg cttcgttcac ttcacggtag gtccattcgc gtcccgcttc ttttattgcg
     6181 ctgcttgcag gcgtccggtt tgcctgtgct tcaaacaaac ggtgaacggc ctcctcttcc
     6241 ggaagcgccg ttttcgtatc attgaatacc tcaacaatct gccgctcttc tttgcctgaa
     6301 acaaggccga gttcccgcac gggcgcgtca ggcgttccga ccgcggcttt catcatgcgg
     6361 gtcagatggt gcgccgttcg ttcgatggca tcatgagcga aagccgctcc attgtatttg
     6421 attttgacag tccacgtttt tcccggatac acgatgagat tgaaatcaaa gctcgtctgc
     6481 tcaaaagcat gttccacttt aatggaaaag ccgagtcttt cctccatgcc cccgttttcc
     6541 agctctttgt ccagcggata gttttcaaac gcgaccaaat gattcaagag gctgccgtcc
     6601 agagcggagc gcttttgaat gtcatagagc ggcacataat catagcgctc ggcttccacg
     6661 gcgtttttct gaactgccgc catcacgtcc gaaaaggctg catctttgtc aatcttgatt
     6721 ctgacgggaa tcgtattgat gaacagcccc gccatcgttt caatgccgtt gatttctgaa
     6781 ggacggcctg atacgacggt gccgaacacc acatcgtctg tatagttgta tttgctgagc
     6841 accgcacccc aaacggcctg aaacaggtta ggccccgtga cttgatgccg gttggcggtc
     6901 tgctgaatct gcgcgaccag ctcctcatcc cagacaaaag aatattcttc attttggtag
     6961 tctttctttt ctgctgccag acggcccggc agcaggctcg gctgttcgaa atccgccagg
     7021 cggctgctcc aatattgttc cgcctcctca taatcccggc ctccgagcca tttgatatag
     7081 tcagagtaag gtttgccctg gcttcccccg gcgggccggc cggcacgaag cgcttcatag
     7141 ttttgaaaca ggcttttcat caaaacgccc atactccagc cgtccatgac gatatgatga
     7201 ttgctccaga cgagatacag ctccttctca ctgagtctga acaccgccgc tttaaagaga
     7261 atatccttcg ccagatgaaa gccctgctgc tgcacgccgc gtttatagcg ttcaatatat
     7321 tcggtttggc gcgcttcatc taattgactg atatcttcat agtgcacctt cgtcttccgc
     7381 tccgccaata cgacttggcg cggtttttga agctgctggt gcacgaatac cgtacgcagg
     7441 atgtcatatg tccggatcag ctcattgacg cttttttcaa aaagctccag ctgaaagctt
     7501 ccgtgaatcc taagctctaa ttgtgtgaag taggaggaag aatgcggatc aagcagcgca
     7561 tgatacagca ttccctcctg catcggtgtg agtgcataca ccttttgaat tgattttttg
     7621 ctcatatgtc acctctgtct tttctgttag agaatttcca ttaatttatc aagttcatca
     7681 agcgtcagat catcatcgcc aagatcgcta ggcgtaagct caggtccgtc ttgggccagg
     7741 cagtgatcta tgattttcaa gaggtgccct ttaaagctgt cactgaacga ttgaatggtt
     7801 ttttcatgaa attcgagaga atgataggca aacgtcatcg tcatctgccc gttttctata
     7861 aatccgacaa tatcaagcgc attcggcttc tctgtgtcag ggctcagcga ttgtcctgac
     7921 ggcatcccgg atcttgtaaa taagccggaa tccgacatct cattaaactg gcctaagtag
     7981 ttaaagctga tatccggatc aatctcaaat gaaagattct ctttatgctc aggcgccgtt
     8041 acataacgca gaataccgta gccgactcct ttgttcggga tgtgtctgat gtcttctttc
     8101 atttgtttca gctgatatga aagatcgtcg gcgtgcttca tctcaaggat catcggatac
     8161 atgctcgtaa accatccgac cgtccgggtg atattcaagc cgtcaagaat gtcttcgcgc
     8221 ccgtggcctt ccaaatgaac gccgatcgtg tcttcacccg tccactcttt cagcgccagt
     8281 ccgagcgccg tgagcaggat gtcgttcatt tcggtgtgat aggcttcatg tacgtttgtc
     8341 gtcagctgtt cagtttgttc aggcgtcaga gagaagctga ccgttctcgt atgtttcatg
     8401 cgctgatcac cagactgcct gtctttcgga agctgagtgc acacctcttt ttcctcaagc
     8461 tctctccagt aatccgcttc tttcagaaac gccttcgtat ttgcgtattc tttgattctc
     8521 tcggcaaatt cggcgaatga atgtgttttc ggcggcagcg ccggttcatt gccttgcttc
     8581 agctgcgtat aaacggacgt gaagtcctcc agtaaaatcc gccaagatac accatccacg
     8641 actaagtgat ggattgcgag aagaaggtgg tctccatctt cagcccggaa gagcccggcc
     8701 ttcagcagat gtcctgtttc cagtgaaata ctgctttgaa gacggtctgc ttccctttcg
     8761 atacggtcct catggtcttc agcaggtccg ttcagctcaa agacgttcag ttcaacagac
     8821 acatcctcaa gtcctctgta atacggtttt tgttcacgat aaatcatccg gagcgcatca
     8881 tggtgcaccg tcaggacatg taatgttttt tctgttatct ccggatcaaa gccgtctttc
     8941 gcgtgaagca tgacggattg attccagtgg tgtttgtc
//
