LOCUS       NZ_JAQEMJ010000013     10210 bp    DNA     linear   CON 22-FEB-2024
DEFINITION  Bacillus velezensis strain TM115-48 GF3HH28002B.Scaf13, whole genome
            shotgun sequence.
ACCESSION   NZ_JAQEMJ010000013
VERSION     NZ_JAQEMJ010000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus velezensis
  ORGANISM  Bacillus velezensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus amyloliquefaciens group.
REFERENCE   1  (bases 1 to 120040)
  AUTHORS   Lin,X., Hu,T., Chen,J., Liang,H., Zhou,J., Wu,Z., Ye,C., Jin,X.,
            Xu,X., Zhang,W., Jing,X., Yang,T., Wang,J., Yang,H., Kristiansen,K.,
            Xiao,L. and Zou,Y.
  TITLE     The genomic landscape of reference genomes of cultivated human gut
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 120040)
  AUTHORS   Zou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2022) BGI Reaserch, BGI-Shenzhen, Beishan
            Industrial Zone, Shenzhen 518083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: MGI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/22/2024 04:06:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,263
            CDSs (with protein)               :: 4,263
            Genes (RNA)                       :: 58
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 69 of 133
            Pseudo Genes (incomplete)         :: 78 of 133
            Pseudo Genes (internal stop)      :: 16 of 133
            Pseudo Genes (multiple problems)  :: 26 of 133
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:15:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10210
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQEMJ010000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10210
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..10210
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10210
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[<0:210](-)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..210)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     aSDomain        complement(<1..204)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_PGW53_RS15025_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="2.20E-11"
                     /identifier="RREFam002.1"
                     /label="PGW53_RS15025"
                     /locus_tag="PGW53_RS15025"
                     /protein_end="70"
                     /protein_start="2"
                     /score="34.6"
                     /tool="antismash"
                     /translation="IKIHKDVIIYKEEGEIILINMMKNKFYTLDEFSSYIWTKFSEYSD
                     VSKLIDFISGKYKIDKKTVKRDI"
     gene            complement(<1..210)
                     /locus_tag="PGW53_RS15025"
     CDS             complement(<1..210)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /locus_tag="PGW53_RS15025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PqqD family protein"
                     /protein_id="WP_270354557.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 8.3e-11,
                     bitscore: 34.6, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 7.2e-10, bitscore: 31.9,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIKIHKDVIIYKEEGEIILINMMKNKFYTLDEFSSYIWTKFSEY
                     SDVSKLIDFISGKYKIDKKTVKRDI"
     misc_feature    complement(235..253)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.85"
                     /tool="antismash"
     misc_feature    complement(238..253)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.47"
                     /tool="antismash"
     misc_feature    446..457
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.85"
                     /tool="antismash"
     gene            complement(736..2115)
                     /gene="rlmD"
                     /locus_tag="PGW53_RS15030"
     CDS             complement(736..2115)
                     /EC_number="2.1.1.190"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="rlmD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388555.1"
                     /locus_tag="PGW53_RS15030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="WP_065180751.1"
                     /transl_table=11
                     /translation="MKMKPPIEKNEYYDVTFEDLTHEGAGVAKVQGFPIFVPNALPEEK
                     AQIKVTRVKKGFAFGRLIELKEESPHRTDAPCPIYKQCGGCQLQHMTYEGQLLFKQKQV
                     KDVLERIGKLDLSNVTVHPTLGMEDPWNYRNKAQVPVGEREGGLVAGFYQQRSHDIIDM
                     SACLIQQSKNDEAVQAVKDICAAYGVKAYNEERHKGWLRHIMVRYGVVTGEMMIVFITR
                     TSDFPHKAKIIEDITAQFPHVKSIVQNINPNKTNVIFGNETNVIWGEEYIYDLIGDVKF
                     AISARSFYQVNPEQTKVLYDKALEYAELQGEETVIDAYCGIGTISLFLAKQAKKVYGVE
                     IVPEAIEDAKRNAELNGITNAEFAVGEAETVIPKWYEEGITADTLVVDPPRKGCDEALL
                     RTIIDMKPKRVVYVSCNPGTLARDLRVLEDGGYATLEVQPVDMFPHTNHVECVAQVILR
                     K"
     gene            complement(2263..3174)
                     /locus_tag="PGW53_RS15035"
     CDS             complement(2263..3174)
                     /EC_number="2.7.1.107"
                     /GO_function="GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388554.1"
                     /locus_tag="PGW53_RS15035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diacylglycerol kinase"
                     /protein_id="WP_015239327.1"
                     /transl_table=11
                     /translation="MKRARIIYNPTSGRELFKKNLAQVLQKFEQAGYETSTHATTCAGD
                     ATNAAKEAALREFDLIIAAGGDGTINEVVNGLAPLDKRPTLGIIPVGTTNDFARALGIP
                     RENILNAADTVINGVARPIDIGQVNGQYFINIAGGGRLTELTYDVPSKLKTMLGQLAYY
                     LKGMEMLPSLRPTEVEIEYDGKLFQGEIMLFLVTLTNSVGGFEKLAPDSSLNDGMFDLI
                     ILKKANLAEFIRVASMALRGDHINDQHIIYTKANRVKVNVSEKMQLNLDGEYGGMLPGE
                     FVNLYRHIHVIMPKEKAEQLDD"
     gene            complement(3327..6470)
                     /locus_tag="PGW53_RS15040"
     CDS             complement(3327..6470)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1049:
                     AcrB/AcrD/AcrF family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388553.2"
                     /locus_tag="PGW53_RS15040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="efflux RND transporter permease subunit"
                     /protein_id="WP_270354572.1"
                     /transl_table=11
                     /translation="MNHIINFVLKNKFAVWLMTIIVTVAGLYAGMNMKQESIPDVNMPY
                     LSVNTTYPGAAPSQVADDVTKPIEQAVQNLEGVSVVTSTSSENVSSVMIEYDYSKDMDK
                     AKTEVAEALDSVSLPDDAKKPDISRYSLNSFPILTLSVTSGKSSLEDLTKNVENTLVPK
                     LEGIQGVASVQVSGQQEEQVEFSFKDKKMKEYGLDEDTVKKVIQGSDVNTPLGLYTFGN
                     KEKSVVVNGNITSIKDLKEMRIPVTSSSAAQGQAGGAGAASAADAQAMQQAQQSASAGV
                     PTVKLSDIADIKDVKKAESISRTNGKDSIGINIVKANDANTVEVADAIKDELNQYKKDH
                     KGFKYSSTLDMAEPITESVDTMLSKAIFGAIFAVVIILLFLRDIKSTMISIVSIPLSLL
                     IALLVLNQLDVTLNIMTLGAMTVAIGRVVDDSIVVIENIYRRMRLKDEPLRGKQLVREA
                     TKEMFKPIMSSTIVTIAVFLPLAMVGGQIGELFMPFALTIVFALAASLLISITLVPMLA
                     HSLFKKSLTGAPVKAKEHKPGRLANFYKKVLHWSLRHKWITSIIAVLMLVGSLFLVPLI
                     GASYLPAQADKTMQLTYTPEPGETKSEAEKAAQKAEDMLLKRKHVDTVQYSLGSQSPLG
                     GSSNGALFYVKYEDDTPDFDKEKDNVLKEIKKTSSRGEWKSQNFSSSGNNNELTYYVYG
                     DSESDIKGTVKDIEGIMKKQKDLKDINSGLSSTYDEYTFTADQEKLSKQGLTASQISQA
                     LMSQTSQSPLTTVKKDGKELDVNIKTEKDQYKSVKELEDKTITSPAGQEVKIGDVAKVK
                     NGTTSDTISKRDGKVYADVTATVTSDNVTKVSSAVQKKVDKLDHPDNVSIDTGGVSADI
                     ADSFTKLGLAMLAAIAIVYLVLVITFGGALAPFAILFSLPFTVIGALAGLYVSGETISL
                     NAMIGMLMLIGIVVTNAIVLIDRVIHKEAEGLSTREALLEAGSTRLRPILMTAIATIGA
                     LLPLALGFEGGSQVISKGLGVTVIGGLISSTLLTLLIVPIVYEVLAKFRKKKPGTEEE"
     misc_feature    6492..6507
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: strong, score: 24.63"
                     /tool="antismash"
     gene            6599..7447
                     /locus_tag="PGW53_RS15045"
     CDS             6599..7447
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014417120.1"
                     /locus_tag="PGW53_RS15045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_063637020.1"
                     /transl_table=11
                     /translation="MNEKKEKIIKTGIHLFAKKGFSSTTIQEIAGECGISKGAFYLHFK
                     SKEDLLLSACEYYIGMSMEEIKKIKTEHQHKPPKDVFRKQIAYQFQEFMEHKDFIILLL
                     SEKVIPENQKVKQYFHEANIQFNMLYRDALLSVYGDAVTPFLADASVMAQGIVSSYIHF
                     LIFNEHTAFRTENVAAFLIARIDDLITGLIRDNPDPLLPEDIFTQPAADRERLLEDIQK
                     IKAQQELPEDVLVSLEVIEEECAKEEPRKPIIKGMLSNLAGSGNEQIETLRASIETYFS
                     L"
     gene            complement(7510..8940)
                     /gene="gatB"
                     /locus_tag="PGW53_RS15050"
     CDS             complement(7510..8940)
                     /EC_number="6.3.5.-"
                     /GO_function="GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /gene="gatB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388551.2"
                     /locus_tag="PGW53_RS15050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="WP_003155730.1"
                     /transl_table=11
                     /translation="MNFETVIGLEVHVELKTKSKIFSSSPTPFGAEANTQTSVIDLGYP
                     GVLPVLNKEAVEFAMKAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDKPIGENGW
                     IEIEVGGKTKKIGITRLHLEEDAGKLTHTGDGYSLVDFNRQGTPLVEIVSEPDIRTPEE
                     AYAYLEKLKSIIQYTGVSDCKMEEGSLRCDANISLRPIGQEKFGTKTELKNLNSFAFVQ
                     KGLEHEEKRQEQVLLSGGVIQQETRRYDEATKKTILMRVKEGSDDYRYFPEPDLVELYI
                     DDEWKERVRATIPELPDERRKRYIEELGLPAYDAMVLTLTKEMADFFEETVNKGAEAKQ
                     ASNWLMGEVSAYLNAEQKELEDVALTPEGLAGMIKLIEKGTISSKIAKKVFKELIEKGG
                     DAEKIVKEKGLVQISDESVLLKLVTDALDSNPQSIEDFKNGKDRAIGFLVGQIMKASKG
                     QANPPMVNKILLEEIKKR"
     misc_feature    complement(9901..9913)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.46"
                     /tool="antismash"
ORIGIN
        1 aatatctctt tttacagttt ttttgtctat tttatatttt ccacttatga aatctatcaa
       61 ttttgaaacg tcactatatt cactaaactt tgtccaaata taagagctaa actcatccaa
      121 agtgtaaaat ttgtttttca tcatatttat taatatgatt tcaccttctt ccttatagat
      181 aatcacatct ttatgaatct ttattctcaa tattagcacc ccaaatattt atatgttgat
      241 aatagttccc atttagtatt gtttgaaatc aattctttaa gatagttttt taattctaac
      301 ggggaaatac gagacaatac tcgttacaaa acgaacgaat atgtaaaata attgatcata
      361 atacatactt tagtaatagt aacatgaaat ttattcctat ggaacagact gaaagtgacg
      421 attttgtgaa caatagaaga gattggaaaa agttttactt actgaagtat tattaataac
      481 actggaatac tactttgatc cgataatgaa ttattttatt ttacaaaaag ggaatatagt
      541 tctgttccag aagatatttt tattaaattt actaataaac attaggcaat aatacctttt
      601 acacactaag ataatctaat agctaatctt tttctctaaa agctgcacaa aaaaagattg
      661 cagaagttac tctaagagta acaccactgc aatccgaaaa aagttttaaa taacgtataa
      721 gaagtttatt tttttttatt ttcttagtat tacttgcgcc acacactcca catgattcgt
      781 atgcgggaac atatccaccg gctgcacttc gagcgtcgca tatccgccat cctcaagcac
      841 ccgcaggtct cttgcaagcg ttcctggatt gcaggacacg tacaccaccc gcttcggttt
      901 catgtcgatg attgtccgca ggagggcttc gtcacagcct tttcttggcg ggtcgacgac
      961 gagggtgtcg gccgtgatgc cttcttcata ccactttgga atgactgttt ccgcttctcc
     1021 gactgcgaat tccgcgtttg tgatgccgtt cagctcagcg ttgcgtttcg catcctcaat
     1081 tgcttccggc acgatttcaa cgccgtacac ttttttcgcc tgctttgcca gaaatagaga
     1141 aatggttccg atgccgcagt aggcgtcgat gacggtttct tccccttgca gctccgcgta
     1201 ctcaagcgct ttgtcgtaga gcacttttgt ctgttccggg ttgacctgat agaacgatct
     1261 ggcggagatg gcgaatttga cgtctccgat gaggtcgtag atatattctt cgccccagat
     1321 gacgtttgtt tcgttcccaa agatgacgtt tgttttgttt ggattgatgt tttgcacgat
     1381 ggatttgacg tgcgggaatt gcgctgtgat gtcttcgatg attttcgctt tgtgcggaaa
     1441 atcacttgtt cttgtgatga agacgatcat catttcgccc gtgacgacgc cgtatctgac
     1501 cataatgtga cggagccagc ctttgtggcg ttcttcgtta taggctttga caccgtatgc
     1561 tgcacagatg tctttgacgg cttggacggc ttcgtcgttt ttggattgct gaatcaggca
     1621 ggcgctcatg tcgatgatgt catggcttct ttgctgatag aatccggcga cgagtccgcc
     1681 ttctctttct cctaccggca cctgcgcttt gtttctgtag ttccacgggt cttccatgcc
     1741 gagcgtcggg tggacggtga cgtttgagag gtccagcttg ccgatccgtt ccaagacgtc
     1801 tttaacttgt ttctgtttaa acaagagctg gccttcatag gtcatgtgct gaagctggca
     1861 gccgccgcat tgtttgtaga tcgggcacgg ggcatccgtt ctgtgcgggc tttcttcctt
     1921 cagctcaatc aagcggccga aagcgaagcc ttttttgacg cgtgtcactt tgatttgcgc
     1981 tttttcctca ggcagggcgt tcggcacgaa gatcgggaag ccctgaacct ttgcgacgcc
     2041 cgctccttcg tgcgtcaaat cctcaaatgt cacgtcatag tattcatttt tttctatcgg
     2101 gggtttcatt ttcatctata atcacctctc gttttaccaa aagccttgtt tcagcgtgct
     2161 ggcaaacctt caaattcaat gtcagccttc aaaggtttcc gatcacgctg aaaagatgac
     2221 aaaatcctaa aacgaatact gttttaggat tttgtccgcg tattaatcat ccagctgttc
     2281 cgctttctcc ttcggcatga tgacgtgaat atgccggtac aggttcacga attcgcccgg
     2341 cagcatgccg ccgtattcgc cgtccaggtt cagctgcatc ttttctgata cattgacttt
     2401 cacacggttc gcttttgtgt aaataatgtg ctgatcgtta atatgatcgc ctctgagcgc
     2461 catgctcgcg actctgataa actccgcgag attggctttt ttcaaaataa ttaaatcaaa
     2521 catgccgtca ttcaggctgg aatcaggggc cagcttctcg aatccgccga ccgagttggt
     2581 cagcgtcact aaaaacagca taatttcgcc ttgaaacagc ttgccgtcat attcaatctc
     2641 gacttctgtc ggtcgaagtg aaggcagcat ttccattccc tttaaataat aagcaagctg
     2701 gccgagcatc gttttcagtt tgcttggcac atcatacgtc agctccgtca gacggcctcc
     2761 gccggcgatg ttgataaaat actgaccgtt cacctgtccg atgtcgatcg ggcgggctac
     2821 gccgttaatg acggtatcag cggcattcag tatattctct ctcggaatgc ccagcgctct
     2881 ggcgaagtca tttgtcgtgc cgaccggaat gatgccgagt gtcgggcgtt tgtctaacgg
     2941 tgcaaggccg ttgacgactt cattaatcgt tccgtctccg ccggctgcga taatcaggtc
     3001 aaactcccgc aaagcggctt cttttgcggc gttggtggca tcccccgcgc atgtggtggc
     3061 atgggtagag gtttcataac cggcttgttc aaatttttgc aggacctggg caagattttt
     3121 cttaaaaagc tcccgtcctg aagtcggatt gtatattatg cgtgcacgtt tcattatctc
     3181 atcatcctac ttaagagtat ccgatccatt attaacacta acaaccttta attatactat
     3241 gacttgagaa aatcgcaaca atatgtacag gaattgccag aagaaaaaag ggcccggctc
     3301 cgtttaagga gccaaggccc gctgtcttac tcttcttccg ttccaggttt tttcttacgg
     3361 aatttcgcta atacttcata tacgatcggc acaatcagca gggtaagcag agtcgaactg
     3421 attaaaccgc cgataacagt gacaccgagt cctttagaaa tgacctggct tccgccttcg
     3481 aagcccaatg ccaatggaag cagggcgccg attgtcgcga tcgccgtcat cagaatcgga
     3541 cgcaatctcg tagagccggc ttccaggagc gcttctctcg tcgataagcc ttccgcttct
     3601 ttatggatga cgcggtcaat taagacaatc gcatttgtga cgacgattcc gatcagcatc
     3661 agcataccga tcatggcatt cagactgatc gtctctccgg atacatacag tccggctaat
     3721 gcgccgatga ctgtaaacgg aagcgagaac agaatcgcaa acggcgctaa tgctccgccg
     3781 aacgtgatca caagaacgag gtatacgatg gcgattgcgg caagcattgc taagccgagt
     3841 ttcgtaaagg aatcagcaat atccgctgat acaccgccgg tatcaatcga tacattgtcc
     3901 gggtgatcca gtttgtcaac tttcttttga acggctgatg acaccttcgt gacgttgtca
     3961 gaagtgacgg ttgccgttac gtcagcgtac actttgccgt cgcgtttgga aatggtatcg
     4021 gatgtcgtgc cgtttttcac tttcgccaca tcgccgattt ttacttcttg gcctgcaggc
     4081 gatgtgatgg ttttatcctc taattctttt acactcttat actggtcttt ttctgttttg
     4141 atgttgacgt caagctcttt gccgtccttt ttcaccgttg tcaaaggaga ctgtgacgtt
     4201 tgggacatca gggcttggga gatttgcgaa gccgtcaggc cctgcttgct gagtttctcc
     4261 tggtcagcgg tgaacgtgta ctcatcatat gtgctggaaa ggcctgagtt gatgtctttc
     4321 agatccttct gctttttcat gatgccttcg atgtctttga ccgtgccttt aatatcagac
     4381 tctgaatctc cgtacacata gtacgttaat tcgttgttgt tgcctgacga actgaaattt
     4441 tgtgatttcc attcgccgcg gctggatgtt tttttgattt cttttaagac attgtctttt
     4501 tctttgtcaa aatcaggcgt gtcatcctcg tatttcacgt agaacaatgc gccgtttgag
     4561 cttccgccga gcggactttg cgagcctaaa gaatactgaa cggtatcgac atgtttgcgt
     4621 ttaagcagca tgtcttccgc tttttgcgca gctttttcag cttcgctttt cgtttcgccc
     4681 ggttcaggcg tgtatgtgag ctgcatcgtt ttgtccgctt gcgcaggcag gtagctcgcg
     4741 ccgatcagag gtacgaggaa caaactgccg acaagcatca gaaccgcgat gatagatgta
     4801 atccatttat gtcttaatga ccaatgcagc acttttttat agaaattggc caatctgccc
     4861 ggcttatgct ctttcgcttt aacaggcgcg cctgtgagcg attttttgaa taagctgtgc
     4921 gcaagcatcg gcaccagtgt gatcgaaatc aacagtgatg cggcaagcgc gaagacgatt
     4981 gttaatgcaa acggcataaa cagttcgccg atctgtccgc cgaccatggc aagcggcagg
     5041 aatacagcaa tggttacgat cgttgatgac atgatcggtt taaacatttc tttcgtcgct
     5101 tcacgcacca attgcttccc gcgtaaaggt tcatctttca gcctcatgcg gcggtaaatg
     5161 ttttcgatca cgacaatcga gtcgtcgact acccgtccga tggcgaccgt catcgcaccg
     5221 agcgtcatta tgttcaatgt gacatcaagc tgattcagca caagaagcgc aatcagcagt
     5281 gacagcggga tggaaacgat agaaatcatc gttgatttaa tatctcttaa gaataagaga
     5341 atgatcacga ccgcaaaaat cgcgccgaaa atcgctttgc ttaacatcgt atccaccgac
     5401 tctgtaatcg gctctgccat atcaagagtc gagctgtact tgaacccttt atggtctttt
     5461 ttgtattgat tcagttcatc tttaatcgca tcggccactt ctaccgtgtt ggcgtcattg
     5521 gcttttacga tattaattcc gatgctgtcc tttccgttcg tgcgggaaat ggattcagct
     5581 tttttcacat ctttaatgtc ggcaatgtca gaaagcttga ctgtcggcac ccctgctgac
     5641 gcgctttgct gtgcctgctg catagcctga gcatccgccg cagatgccgc accggctccg
     5701 cccgcttgac cttgagcggc agaagaagat gtgaccggaa ttctcatctc tttcagatct
     5761 ttaattgacg taatatttcc gttaacgaca actgattttt ctttgttgcc gaatgtgtac
     5821 aatccaagcg gcgtattgac gtcagagcct tggatcactt tcttgacggt atcttcatca
     5881 agtccgtact ctttcatttt tttgtctttg aaagagaatt ccacctgctc ttcctgctgt
     5941 ccggaaactt gtacggacgc aacgccctga atgccttcaa gcttaggaac gagagtgttt
     6001 tccacatttt tagtcagatc ttcaagcgag cttttgccgc ttgtcacact gagcgtcaaa
     6061 atcgggaaag agttgaggct gtaacgggaa atgtcaggtt ttttcgcgtc gtcaggaagg
     6121 ctgacactgt ccagcgcttc agcgacttcg gttttcgcct tgtccatatc cttgctgtaa
     6181 tcatattcaa tcataaccga tgagacgttt tcagaagatg tggacgttac gacgctcact
     6241 ccttctaaat tctggaccgc ctgctcgatc ggttttgtca cgtcatccgc aacttgactc
     6301 ggagcagctc cgggataagt cgtatttact gaaaggtaag gcatgttgac gtccggtatg
     6361 gattcctgct tcatattcat accggcatac agccctgcta cagtaacgat aatcgtcatc
     6421 agccaaactg cgaatttgtt cttcagtaca aagttaataa tgtggttcat catgtcctcc
     6481 gttaattttt attgactgac tggtcattct atataatact aaacggtata actcaacatc
     6541 gtcaactaat aaggcagtaa atttttctaa atttttcagg attaagggga agcacgtcat
     6601 gaacgaaaaa aaagagaaaa tcataaaaac aggcatccat ttatttgcca aaaaagggtt
     6661 ttcttcaaca accattcagg aaatcgccgg tgaatgcgga atttcaaaag gagccttcta
     6721 tctccatttc aaatcaaaag aagacctttt gctgtcagca tgtgagtatt acatcggcat
     6781 gtctatggaa gaaataaaga aaatcaaaac agaacatcag cataaaccgc ctaaggacgt
     6841 atttcgaaaa caaatcgctt atcaattcca ggaatttatg gagcacaagg actttatcat
     6901 tctcttgctg agcgaaaagg tcattcctga aaatcaaaag gtcaaacagt attttcatga
     6961 ggccaatatt caatttaaca tgctgtatcg ggatgcgttg ctctccgttt acggagatgc
     7021 cgtcacaccg tttttagcgg atgcgtccgt tatggcgcaa ggaattgtga gctcctatat
     7081 tcatttcttg atttttaatg aacatacggc attccggacg gaaaacgtgg cggcctttct
     7141 gattgccaga atcgatgatc tgattacggg actgataaga gacaatcccg atccgctgct
     7201 gcctgaggat atcttcactc agcccgcggc ggacagagaa aggctgcttg aggatattca
     7261 aaagataaaa gcacagcagg agctgcctga ggacgtcctc gtatcgcttg aagtcattga
     7321 agaggaatgc gcaaaggaag agccgcgaaa accgattatt aaaggaatgc tgtccaattt
     7381 ggcaggcagc ggaaatgagc agattgaaac gctgcgggct tccattgaaa cctatttcag
     7441 cttataaaaa aagagaccgg cgcttaccgg tctccgtgtc agcagcgccc ttagaggcgg
     7501 ctgctttttt tatcgttttt tgatttcttc aagtaagatc ttgttgacca tcggcgggtt
     7561 ggcctgtcct tttgaggctt tcataatctg cccgacaagg aagccgatcg cgcggtcttt
     7621 tccgtttttg aagtcttcaa ttgactgcgg gttgctgtca agcgcatctg ttaccagctt
     7681 cagaagcacg ctttcgtcag aaatctgcac gaggcctttt tctttgacga ttttttctgc
     7741 gtcgccgcct ttttcaatta attctttaaa gactttcttc gcgattttgg acgagatggt
     7801 tcctttttca atcagcttaa tcatgccggc aaggccttca ggagtcagcg cgacatcctc
     7861 aagctctttt tgttcggcgt tcaggtaagc tgagacttca cccatcagcc agtttgatgc
     7921 ctgcttggct tctgcgcctt tattgaccgt ttcttcgaag aaatcagcca tttctttcgt
     7981 cagcgtcaga accattgcgt cataagcagg caggccgagc tcttcgatat aacgcttgcg
     8041 gcgctcgtcc ggaagctcag gaatggttgc tcttacgcgt tctttccatt cgtcatcaat
     8101 gtagagctcg acaaggtcag gctcagggaa ataacgatag tcgtccgaac cttctttgac
     8161 acgcatcaaa atggtttttt tcgtcgcttc atcgtagcgt cgcgtctcct gctgaatgac
     8221 tccgcctgac agaaggacct gctcttggcg tttttcttca tgctcaagtc ctttttgcac
     8281 gaacgcgaag gagttcaggt ttttcagctc cgtttttgtg ccgaatttct cctggccgat
     8341 cggacggagg gaaatgttgg cgtcacaacg cagtgaacct tcttccattt tacagtcgga
     8401 aacgcctgtg tattgaataa ttgatttcag cttttcgaga tatgcgtaag cttcctccgg
     8461 tgtgcggata tccggctcag atacaatctc gacaagcggc gtgccctggc ggttgaagtc
     8521 aacgagggaa tagccgtcgc ccgtatgcgt cagttttccc gcatcttctt caaggtgaag
     8581 acgggtaatg ccgatttttt tcgtttttcc gccgacttcg atttcgatcc agccgttttc
     8641 gccgatcggc ttgtcaaatt gagaaatttg atatgccttc gggttgtccg gatagaaata
     8701 gtttttccgg tcaaacttcg tatccgttgc gatttcacag ttcaaagcca ttgcggcttt
     8761 catcgcgaat tcaaccgctt ctttgttcaa aacgggcagc acgccgggat agccgaggtc
     8821 gatgacgctc gtttgggtat tcgcttccgc gccgaatggc gtcggtgagc ttgagaaaat
     8881 tttagatttt gtttttaatt ccacatggac ttcaagtccg attaccgttt caaagttcac
     8941 ttcatttcac cccttacagt tcaggtttcg ctttatgatg atctgttgcc tgctcaaaag
     9001 catgagcgac gcggtataca gtgccttcat cgaagtgttt tccgatgatt tgcaatccga
     9061 gcggcagccc gtccgcaaat ccgcacggca cgctgattcc cggcacaccc gcgaggttga
     9121 cagggatcgt taaaatatcg ttggcgtaca ttgtgagcgg atcgctggtt ttttcgccga
     9181 ttttgaatgc cggtgtcggc gttgtcggtc cgacgatgac atcgtatttc tcaaatacgt
     9241 cttcaaaatc tttcttaata agcgtgcgca ctttttgcgc ttttttgtaa tatgcatcat
     9301 agtagccgga gctgagcgcg aaggtgccga gcatgatgcg gcgcttcact tcattgccga
     9361 atccttcaga gcgtgtctgt ttataaaggt cgatcaggtt gtccgcattg tctgagcggt
     9421 agccgtagcg gatgccgtca aagcgcgcaa ggtttgcaga cgcttcggaa gaagacagca
     9481 gataatacgt cgcaagcgcg tatttgctgt gcggcagaga tacttcttcc catgtcgctc
     9541 cgaggtcttc aagcactttt aaagcggcta aaaccgattc tttcgcttct ttgccgacgc
     9601 cttctccgag atattcttgc ggtacggcga tttttaagcc tttaatatcg ccagttaatg
     9661 aagaaagaaa atccggcact tcgacattgg cgctcgtaga gtccattttg tccgggcctg
     9721 aaatcgcctg aagcaggaat gcgttatctt ctacggttct tgtgatcggc ccgatctggt
     9781 ccagtgaaga cgcaaaagcg accaaaccgt aacgcgacac gcgtccgtat gtgggcttta
     9841 atccgacgac gccgcagaaa gaagccggct ggcggatgga gccgcccgta tcagatccga
     9901 gtgaaaacgg cacttcgccc gcggcaaccg cagctgcgga accgccgctt gatccgccgg
     9961 gaaccgtatc gagattccaa gggtttttcg tcgctttgta cgcggagttt tcagtagaag
    10021 aacccatcgc gaactcgtcc atattcagct ttccgatcgt gacggcttca gcggcttgaa
    10081 ggcgctccac gaccgtcgca tcgtaaatcg gatcgaagtt ttcgaggatt ttgcttgagc
    10141 atgttgtgcg aagccctttg gtcacgatgt tgtctttgac gccgatcggc attccgaaga
    10201 gaagaccgtg
//
