LOCUS       NZ_JAQEAZ010000004     24853 bp    DNA     linear   CON 29-FEB-2024
DEFINITION  Blautia hansenii strain AM27-24LB AM27-24LB.Scaf4, whole genome
            shotgun sequence.
ACCESSION   NZ_JAQEAZ010000004
VERSION     NZ_JAQEAZ010000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Blautia hansenii
  ORGANISM  Blautia hansenii
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Blautia.
REFERENCE   1  (bases 1 to 190354)
  AUTHORS   Lin,X., Hu,T., Chen,J., Liang,H., Zhou,J., Wu,Z., Ye,C., Jin,X.,
            Xu,X., Zhang,W., Jing,X., Yang,T., Wang,J., Yang,H., Kristiansen,K.,
            Xiao,L. and Zou,Y.
  TITLE     The genomic landscape of reference genomes of cultivated human gut
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 190354)
  AUTHORS   Zou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2022) BGI Reaserch, BGI-Shenzhen, Beishan
            Industrial Zone, Shenzhen 518083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. v2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: MGI
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/29/2024 10:24:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,933
            CDSs (total)                      :: 2,870
            Genes (coding)                    :: 2,807
            CDSs (with protein)               :: 2,807
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 15 of 64
            Pseudo Genes (incomplete)         :: 49 of 64
            Pseudo Genes (internal stop)      :: 7 of 64
            Pseudo Genes (multiple problems)  :: 8 of 64
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:43:40
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 24853
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAQEAZ010000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..24853
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /candidate_cluster_numbers="3"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /product="cyclic-lactone-autoinducer"
                     /region_number="1"
                     /rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /rules="(TIGR04223 or AgrB)"
                     /tool="antismash"
     cand_cluster    1..24853
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="neighbouring"
                     /product="lanthipeptide-class-i"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     cand_cluster    1..18966
                     /candidate_cluster_number="2"
                     /contig_edge="True"
                     /detection_rules="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..18966
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2784:8966](+)"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2785..8966
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(((Lant_dehydr_N or Lant_dehydr_C) and
                     cds(LANC_like and not Pkinase)) and not (YcaO or
                     TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     gene            86..259
                     /locus_tag="PGT18_RS05860"
     CDS             86..259
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03731.1"
                     /locus_tag="PGT18_RS05860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="gallidermin/nisin family lantibiotic"
                     /protein_id="WP_270387631.1"
                     /sec_met_domain="TIGR03731 (E-value: 1.1e-09, bitscore:
                     31.8, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKFDDFDLDIIEKKVRRDIILASITSQHSFCTPNCPTGFLCPPK
                     TQLTCTCKLKGR"
     CDS_motif       86..160
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MAKFDDFDLDIIEKKVRRDIILASI"
                     /locus_tag="PGT18_RS05860_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       161..259
                     /prepeptide="core"
                     /locus_tag="PGT18_RS05860_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="-0.20"
                     /molecular_weight="3354.9"
                     /monoisotopic_mass="3352.7"
                     /core_sequence="TSQHSFCTPNCPTGFLCPPKTQLTCTCKLKGR"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MAKFDDFDLDIIEKKVRRDIILASI"
                     /leader_location="[85:160](+)"
                     /alternative_weights="3373.0"
                     /alternative_weights="3391.0"
                     /alternative_weights="3409.0"
     gene            403..1068
                     /locus_tag="PGT18_RS05865"
     CDS             403..1068
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543482.1"
                     /locus_tag="PGT18_RS05865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_270387633.1"
                     /transl_table=11
                     /translation="MEKILVIDDDEKILELVQEILKNEHYIVETRSYVDNTNIGEFEGF
                     DLILLDIMLPFLDGYEILERIKNIITCPVIFLSAKSSEGAKVKGLMSGADDYITKPFSN
                     RELVARVKVALRRNVKNESNGILINGLVFNQDSNSIKLDNGEILLTKNEFRICKILVQN
                     SGKIFSKDELYEYLYDLDANAQLRTITEFIYSIRKKFKTLGLDPIKTIWGIGYKWDIK"
     gene            1053..2411
                     /locus_tag="PGT18_RS05870"
     CDS             1053..2411
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="PGT18_RS05870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_270387635.1"
                     /transl_table=11
                     /translation="MGYKIITMKRQLLLYLIKLFVGIVVIIICWLALTNIFVECGVIIP
                     ANYSETILEENRKRLDDIQQITDNDLPYGSKYSIFDLDYNYERGTMNKSDIEVTKKILL
                     GKESNLQGNYVYSVIARTEEYCVIKYNIKARFNSDNKIFNYIDYDNLSYITMVLVFLLY
                     VYIMTLHLVSIWKNNFEKIEKITLEIEKQNLDFTYEESKIKEFSNIITALIKMRDALKE
                     SLYQTWKIENEKNEEIAALAHDIKIPLTIISGNTELLKCYSSDEYSLSHLQSIWGAVGK
                     MEEYVNLLIKYVKADRIDFHEKESMPCNSFSHKIVSEIKEYISGSEEIIKFNVEKVRGT
                     IKIDYISLERAIFNIIDNAIEYKVYGDKIMCFIGQKDDSYIFTICNEKGEFSSQVLENG
                     TKLFFTSNNNRNSIHYGIGLAYANKVIQSCEGELELYNSKEHGAVVSIKLPIT"
     gene            complement(2460..2663)
                     /locus_tag="PGT18_RS05875"
     CDS             complement(2460..2663)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGT18_RS05875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_270387637.1"
                     /transl_table=11
                     /translation="MKTKEELLLSVKENIEAFGKDKLVYAHMSLQNEGTILYGYSYTPE
                     GKFIDEASLEELLIILESDTDM"
     misc_feature    2731..2745
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.15"
                     /tool="antismash"
     gene            2785..5844
                     /locus_tag="PGT18_RS05880"
     CDS             2785..5844
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155: Lantibiotic dehydratase domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /locus_tag="PGT18_RS05880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lantibiotic dehydratase"
                     /protein_id="WP_270387638.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.5e-126,
                     bitscore: 415.8, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 1e-13,
                     bitscore: 45.4, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 9.8e-31, bitscore:
                     100.9, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLYYNTQKFMFRIPNDVERKLDFTEEEIKNACLDAKFREKVSI
                     ASPALVDMMDLYIKNPEKLSEKKLVNFQNSIMKYLIRSKNRTTPFGLFSGVGLGKFGDS
                     DTFDVSGAKYQKKVNIDSEWLFGFISQLEKIKSQRLRFKINDACYIKGNRAILLYSTEK
                     EIEEISVRATRVFEIIYESCQEFKEYNQIARIIEEEYPNVPNEKVTFYLNELISKEILI
                     SDLRPSLNSRDQIAYVIERLRESALFEEAGNIIEISKMCTSYMNLPVGEGITLYDKIVS
                     KMKLLYSCSSYLQVDTVIENAVFEIKSTVANKINRLASFFVYISNDKNESHTYLDEYRN
                     KFIEKYGVDREVPLLEMLDSNIGIGAPTSYLNPQNDFFEEDSTKPNYNLRLKNYLLNKY
                     ESAITNKTSITLEQDEIEGILKREIKTDEVPISLELYFQLKKKNDELNLCLGPNCGSFV
                     AGKTFGRFSTISDEFADMLEDINKEERRLRDDNIEMCEIGFLPAPVRNGNIVRTRTFRE
                     KKTVIFTAADKGTTDVINIKDISIGVFNELFYARDYKTKKLVVFESNNMYNPMLNPNIL
                     RFLQDISHEGKRSWSEFPWTYIFSEFRHVPAIKFEDIVIENEKWKLNLSEMRLEKKNFE
                     EFKCKFLQLIKDKNIPDDIYLTEADNRIKLDLKKELSIRIIFDEFKKHGSRDLILERAE
                     AGENITYSGEGGHTTEIVVPLFRKEKELQNVYPAEKVIIERKKHLELPFENWLYFNLYC
                     NSNREDELIAFDIMDFCEELKKKYDVDYFFMRYVDPKPHVRLRIKGTQEVLLQIYPLII
                     KWQHQLLDDGIIGDLKISIYDREIERYGGLHLMDIAEQVFFIDSFIVESILRMKRLGVL
                     AMDQEDIAIISIIMYTQGFYETFEEQMNFLAINYHTSDFMSEFKKKKQRLVSLCGCEND
                     WKELLSNEEGTSLYNLLNMRTVVLNKYRDEINNINQDPLFKNGIVASVIHLHCNRIIGT
                     DRELERKLMAFSESIMYAKRYIMKRNEDHE"
     aSDomain        3211..3468
                     /aSDomain="Lanthipeptide_LanB_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing LanB protein in a class I
                     lanthipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_PGT18_RS05880_Lanthipeptide_LanB_RRE.
                     1"
                     /evalue="1.60E-14"
                     /identifier="RREFam001.1"
                     /label="PGT18_RS05880"
                     /locus_tag="PGT18_RS05880"
                     /protein_end="228"
                     /protein_start="142"
                     /score="45.4"
                     /tool="antismash"
                     /translation="KINDACYIKGNRAILLYSTEKEIEEISVRATRVFEIIYESCQEFK
                     EYNQIARIIEEEYPNVPNEKVTFYLNELISKEILISDLRPS"
     cand_cluster    4112..24853
                     /candidate_cluster_number="3"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /kind="single"
                     /product="cyclic-lactone-autoinducer"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    4112..24853
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[14111:14853](+)"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      14112..14853
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="2"
     gene            5837..7639
                     /locus_tag="PGT18_RS05885"
     CDS             5837..7639
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543486.1"
                     /locus_tag="PGT18_RS05885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_270387639.1"
                     /transl_table=11
                     /translation="MNNKKVGFKEVWRALKQIPKTITIIRKVDKKGFTLIVMLSMIAGA
                     FPVITLVLSQELINSIVKKSSPLRYTIILFTLYIIVSFLGSILGEAKDYIESKFQYVLQ
                     YKLNYLIMDKCTKLTLKDFETPAMYDKIEKITGEVAYKPYQIMIAIIGIITSVVTMLSS
                     AFLLFAWNPYISMLLLVVPIISMLYYLKIGQQEFEIMWNRAKDERKTWYLTYLLTHDFS
                     FKEISLLDLKDYFLKKYWSISALFIRQNTSILKRKTLFNILYELSIQIVSAIIIGVAIM
                     SAYVGEILVGNVVSYIRSVALVQTNSQAIMSNIYTVYSSTLYMDMLFQFLNYCVKEDSS
                     EKIAMKEPINKIDIKELSFSYNGKTKVLDKINLTLVQGEKVALVGPNGSGKSTFLKILA
                     GLYNIEEGEILINGRSLKDLDIRSYRSKMSVLFQDFVKYELSLRENIGFGDIQNLNNDE
                     KMVQMLHKLQTKFLQTDNGSYNLDLQLGNWFEDGCQLSQGQWQKIALGRVYFRDASFYI
                     LDEPNAALDTVSEREVFEKFFELSKDKIGVFISHRLNAAKMADKIIVMDQGKIVGIGKH
                     DELLRNCMTYQKLYKAESYEREDM"
     gene            7644..8966
                     /locus_tag="PGT18_RS05890"
     CDS             7644..8966
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140: Lanthionine synthetase C family protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /locus_tag="PGT18_RS05890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lanthionine synthetase C family protein"
                     /protein_id="WP_270387640.1"
                     /sec_met_domain="LANC_like (E-value: 2e-65, bitscore:
                     214.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MECDILTMTKNIATNLSDYERMLKIMGRKENQRTWEGIEFEAFSP
                     LTLSHGLPGICLLYGKLMECCPKEDKWGRFAHNYLSLMVEELNKKGFQSLSMFSGAAGV
                     ALSVASVSNDFLNYNKLLNTLNDYILNRFEESYSNIVKEEGTHSLYYDVIEGLSGVLSY
                     LGIYRNQRKYYNVITRGLEILVNLTKDIEIQGQVVPGWYIPVQNQFSELEQDLYPKGNF
                     NTSLSHGIAGPLTILSDMMANGIIVDGQEEAIIKIIKFLFDFKLNDGERDFWKGQIDFD
                     EVVNKKLTDENIIRRDAWCYGNPGICYAIVSAGNALDNQLLIDYGIENLRLTMKNIKGI
                     FSPTFCHGYAGLYQVLNSVEMLLQKEFFSLEKELLKNKITDFYNPDYIYGFKNMELDNK
                     MEKIKPYDVCGLLEGTTGVCLSLLEGEKKSDNLWKKAFLLA"
     gene            9076..9432
                     /locus_tag="PGT18_RS05895"
     CDS             9076..9432
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543488.1"
                     /locus_tag="PGT18_RS05895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_270387641.1"
                     /transl_table=11
                     /translation="MDKITHKVRCEQWTNIIKECLASGMPKTTWCREHGISGKSFFYWQ
                     RILREEAYLSTLENTLTPAVKENSVPTTTDFVEIKMTDHTSSSASPFKPDVAIRRESVS
                     IEISNTATEERTSK"
     gene            complement(9734..9961)
                     /locus_tag="PGT18_RS05900"
     CDS             complement(9734..9961)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421672.1"
                     /locus_tag="PGT18_RS05900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_270387642.1"
                     /transl_table=11
                     /translation="MAREINAELLDTKIEKAQQDLAKAKHRYDAAAATLKDLLDKRDAL
                     RQKKLLDAIAQSGRSYEEIKQYLHSKSEEV"
     gene            complement(9964..10299)
                     /locus_tag="PGT18_RS05905"
     CDS             complement(9964..10299)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGT18_RS05905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_270387644.1"
                     /transl_table=11
                     /translation="MRSHTNEGVEGRIFTQFIALIIRNKIYTALQEENEKLERKQNYMT
                     VPAVIKELEKIEMTRQTDNVYRLDHAVTAKQKKILKAFGMNEGNITYRVGEISNTLKKS
                     NIQKERR"
     misc_feature    10286..10297
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            11074..11448
                     /locus_tag="PGT18_RS05910"
     CDS             11074..11448
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337551.1"
                     /locus_tag="PGT18_RS05910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_117638661.1"
                     /transl_table=11
                     /translation="MESRIKQLREKRGLIQEILAAELGITQQMLSKYERDVTLIKVDIL
                     KKIATYFNVTTDYLLGVSDVKRDLQRQMKMNETLDEYYDLIEIYKDLDSYDKEMIWSIM
                     QTVKKVGEKRKKDNKLIEES"
     gene            11454..12182
                     /locus_tag="PGT18_RS05915"
     CDS             11454..12182
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543495.1"
                     /locus_tag="PGT18_RS05915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_270387645.1"
                     /transl_table=11
                     /translation="MLSIAICDDDIQTTGQIEMMIQKIAKRNFADVDIEVFWNGESLAD
                     SVVAGDGYDIIYLDIEMNNEDGISTAKRIRKYDKNVLIIYVTSHENYMKESFSVRPFQF
                     LVKPVSEKQMETCFMAAYEDINSGDFYFRYSYQRMNHKVLIRDILYFESSKRKVFIVTR
                     EETFEFYGKLNEIENSLKVCKISFLRVHQSFLVNYKHIKGQSYDFVVMDNEKKISISED
                     RRKLISEQYCSMEDTYYVDR"
     gene            12112..13476
                     /locus_tag="PGT18_RS05920"
     CDS             12112..13476
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543496.1"
                     /locus_tag="PGT18_RS05920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_270387646.1"
                     /transl_table=11
                     /translation="MKTEESLSASSIVRWRIHIMWIDKALPYGADILLSAYAVYLFFYY
                     FDIFFARKKSKKLWVAGLTEFVLWQFGISTIISLPAYVNIAVTVIVTLFAVTITYEGMW
                     WNKCVFVITFNAIWMLMETLSNYVLMIYCEQYASVQSIGSFVSKSFFLAVIIALKKVFT
                     DDEIKELSVRYSIMLVLIPMGSIYIMNNVFMLGFTVNDNHTRINSAITVIILLGMNILI
                     FYIYMKLADDLRLRHMASVYEQQLELCERHQQEREISILQLRDAKHNMKNNLVSILAYA
                     ENRQHEKIIGFINEIMEESGMAIAAISNSGNIVIDSLIGYWYVTAKKKEIDFQTDICIP
                     MMLPFKGADICLILGNLLENAVEAAQKVDGKRYIEIKMKYDKNNILLSVTNSYSGKLLK
                     TKDHRLKSTKTDAENHGVGLASVYRAVAKYCGTIIIDDSVFGQFKIRVLLYGNQE"
     gene            13576..13965
                     /locus_tag="PGT18_RS05925"
     CDS             13576..13965
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGT18_RS05925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_118404099.1"
                     /transl_table=11
                     /translation="MEKILLFSDDEKLFEITKQVTEGKQELIWGNYQCLETNGYPFADV
                     VIMHFDEDKIRKDILQPIIKVKGRLGHLTPILAVIENGSMQDIFSVLKIGVYDYIETID
                     DLEKYQKKIQDIILWDWYLKKYKGG"
     gene            14112..14261
                     /locus_tag="PGT18_RS05930"
     CDS             14112..14261
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: TIGR04223"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543498.1"
                     /locus_tag="PGT18_RS05930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="WP_118591490.1"
                     /sec_met_domain="TIGR04223 (E-value: 2e-16, bitscore: 53.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLKDVKKQSAKMLASVSLKLGKMSADSACCYIYHQPKMPEELKN
                     MKQS"
     gene            14263..14853
                     /locus_tag="PGT18_RS05935"
     CDS             14263..14853
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421682.1"
                     /locus_tag="PGT18_RS05935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="accessory gene regulator B family protein"
                     /protein_id="WP_270387647.1"
                     /sec_met_domain="AgrB (E-value: 1.8e-32, bitscore: 105.7,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQLSEKITKYVIKTGAVSPELYVVYQYGFQIGLEMLCCFATCFG
                     IAIYMHMIPEFVVFTGIFILLRTYAGGVHLNSFGACYICSVAVQTLVMYFNNKYKFTVS
                     IAWALILMSAILILKAAPVESINRELNNTEKEHCKKVTMKILTGVIAFAICCTVGLIQD
                     MLSLIALTTFIILISQYVGILKYKIENSKNKRR"
     misc_feature    complement(15065..15079)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.7"
                     /tool="antismash"
     gene            15100..15552
                     /locus_tag="PGT18_RS05940"
     CDS             15100..15552
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613389.1"
                     /locus_tag="PGT18_RS05940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_270387648.1"
                     /transl_table=11
                     /translation="MEQSSSYDEKTVRHQFDRKCKLALKGEVVDYERHLAYLRKHEVLF
                     SELSEREIENFSIINEYALEKSYFRAGGYDVVVKDALLAEALKALTERKRDVILLSYFM
                     EMNDADIARKLNLVRSTVHEHRTRSLEILKAMLEENVNENGRKKCK"
     gene            15524..15775
                     /locus_tag="PGT18_RS05945"
     CDS             15524..15775
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613390.1"
                     /locus_tag="PGT18_RS05945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_270387649.1"
                     /transl_table=11
                     /translation="MKTEEKSVNNKKLIPLCVIERALEGDIQAINKVLKHYEGYIAKLS
                     IRKMYDEYGSVYYGVDETLRRRLETKLIKTILGFKMVR"
     misc_feature    15790..15807
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.23"
                     /tool="antismash"
     gene            16163..16312
                     /locus_tag="PGT18_RS05950"
     CDS             16163..16312
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613393.1"
                     /locus_tag="PGT18_RS05950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SHOCT domain-containing protein"
                     /protein_id="WP_117944158.1"
                     /transl_table=11
                     /translation="MNSTKLLRYSMQLSMLKQLRTLKLINENEYRLIEKKLKKDYGVIS
                     NITA"
     gene            16382..18151
                     /locus_tag="PGT18_RS05955"
     CDS             16382..18151
                     /GO_function="GO:0000150 - DNA strand exchange activity
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540999.1"
                     /locus_tag="PGT18_RS05955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="recombinase family protein"
                     /protein_id="WP_270387650.1"
                     /transl_table=11
                     /translation="MEVEIIKANSSGEDISNGRRIDRLRVAAYCRVSTDDEDQIKSYNS
                     MVRYYTDLIKSNKQWVFAGVFADKAITGTKTDKREEFQLLIQECLSGNIDMVIAKSIPR
                     FARNTLDTLKYVRMLRERNIAVYFEVEKINTLKDGEFLLTILSSVAQQEVENTSAYVKK
                     GLKMKMKRGELVGFQGCLGYDYDVVTKSLSVNEEGAETVRYIFDRYVAGAGSTMIAREL
                     NEQGIPTIKGNSWTSSSVMGIINNEKYKGDILLGKTFTVDPISKRRLENLGEEDRFYIK
                     NHHEAIISEETFARAQEIRERRNGGRKKGVAPGKREKFSRQYAFSCMLECGFCGANLSR
                     RRWHSSSKYTKTIWQCVESTKHGKRFCPDSKGIPEQVIEDAFIESYRMLCMDHKDVLEE
                     FIKRVEKTLLEDSIEDKIEKLNRSVYNIQYKRKKLLENYLEGVVAKDIYEETDMGYEKK
                     LSEAKSQLSMLEQQYDNEGSLQRRLAEFKKVLSKNQVLEEFDRGIFESTIEKVIVGGYD
                     ENGEKDPYKIIFIYKTGFKNEIGNAKERFGKKSKAVEKAKEMCSHIVDEVKDMCSYVSD
                     NTCGDGCSDDVLW"
     misc_feature    18188..18197
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     gene            18355..>18651
                     /gene="tnpA"
                     /locus_tag="PGT18_RS05960"
                     /pseudo=""
     CDS             18355..>18651
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tnpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611256.1"
                     /locus_tag="PGT18_RS05960"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS200/IS605 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDKNSLAHTKWECKYHIVFAPKYRRKVIYKQIRADIGHILSELCK
                     RKGIEIIEAEACPDHIHMFVRIPPKYVFERPEQCTGTMLSSDWYHRKGTTSVLL"
     gene            complement(18756..19004)
                     /locus_tag="PGT18_RS05965"
     CDS             complement(18756..19004)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221196.1"
                     /locus_tag="PGT18_RS05965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_022239852.1"
                     /transl_table=11
                     /translation="MAETEKMDISYIGPIFKKYRVDANRTQEEVAEKVGITARFLMALE
                     NEEKRPSIDIHLRQYMRLNDRDKSVIRTAIQEMLNNR"
     gene            complement(19203..20831)
                     /gene="hcp"
                     /gene_synonym="priS"
                     /locus_tag="PGT18_RS05970"
     CDS             complement(19203..20831)
                     /EC_number="1.7.99.1"
                     /GO_function="GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hcp"
                     /gene_synonym="priS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221193.1"
                     /locus_tag="PGT18_RS05970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hydroxylamine reductase"
                     /protein_id="WP_022239853.1"
                     /transl_table=11
                     /translation="MEQKMFCYQCQETAGCKGCTMSGVCGKKPDVAAMQDLLVYVTKGI
                     SAVTTALRQEGKQVSAEINHLITLNLFTTITNANFDKENIEAIIRATLIEKDVLLAQIA
                     DPSGLPKAAKWNGSGNWEEKAATVGVLSTENEDIRSLRELITYGLKGLSAYSKHANVLL
                     KDDEEVDAFLQRALAATLDDNLSVEELIALTMETGKHGVSGMALLDKANTEAYGNPEIT
                     KVNIGVGTNPGILVSGHDLRDLEMLLEQTQGTGVDVYTHSEMLPAHYYPAFKKYPNFIG
                     NYGNAWWKQKEEFESFNGPILMTTNCIVPPKDSYKDRLYTTGAAGYPGCTHISGEIGEQ
                     KDFSVIIEHAKRCATPTEIETGELIGGFAHAQVLALADQVVDAVKSGAIKKFVVMAGCD
                     GRAKSREYYTEFAKALPKDAVILTAGCAKYKYNKLPLGDINGIPRVLDAGQCNDSYSLA
                     VIALKLKEVFGLDDINDLPIAYNIAWYEQKAVIVLLALLYLGVKNIHLGPTLPAFLSPN
                     VAKVLVENFGIAGISTVEEDLKFFF"
     gene            complement(20911..21099)
                     /locus_tag="PGT18_RS05975"
     CDS             complement(20911..21099)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847359.1"
                     /locus_tag="PGT18_RS05975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ferredoxin"
                     /protein_id="WP_004221192.1"
                     /transl_table=11
                     /translation="MKIKVDQNLCIGCGLCCGICNEVFRMNEHEKAEAYQPANDDNQSA
                     VQDAIDSCPVSAIAWED"
     gene            complement(21173..21886)
                     /locus_tag="PGT18_RS05980"
     CDS             complement(21173..21886)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842261.1"
                     /locus_tag="PGT18_RS05980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="4Fe-4S binding protein"
                     /protein_id="WP_004221191.1"
                     /transl_table=11
                     /translation="MIRKIIQIDEEKCNGCGACAEACHEEAIGMVNGKAKLLRDDYCDG
                     LGDCLPTCPTGAISFVEREAAAYDEKAVQENMRKKNRMNSPSVGVHAGCPGSRMQRIQH
                     SQESAPSAPMQAELQLRQWPCQIKLVPVGAPYFEGAKLLIAADCSAYAYARMHEDFMRG
                     KVTLIGCPKLDNVDYSEKLTQIIQNNNIQSVTIVQMEVPCCGGLELAAKKALQASGKFI
                     PWQVVTISIDGKILE"
     gene            21978..22640
                     /locus_tag="PGT18_RS05985"
     CDS             21978..22640
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221190.1"
                     /locus_tag="PGT18_RS05985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_270387651.1"
                     /transl_table=11
                     /translation="MNLSNTQLFRGLEEAEITSLLDCLNATTRSFQKGEVILSEGNITE
                     NIGILLSGLAVISCNDIWGNTSILGHVAPGSVFAEVYACIPGEPMLVTVSAAEDTSILF
                     MNVGRVLTTCTNACPFHTRLVRNLLTVCAHRNLQLSQRIQHTSSKSIRGRLMSYFSECA
                     KRAGSNSFLIPYNRQQLADYLNVDRSTMCNELSKMQKDGIIEYSKNQFLLKDSLMFQ"
     gene            complement(23064..23351)
                     /locus_tag="PGT18_RS05990"
     CDS             complement(23064..23351)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGT18_RS05990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_004221186.1"
                     /transl_table=11
                     /translation="MLNDGSGFKKVYLATGFTDLRKGIDGFAIGLSSFSNSCENEGQAA
                     DSGSGIKFWRNAKKRLHRHKSLHRRLIRIFHVLIVPLVVRNIPIAGSRFF"
     gene            complement(23341..23589)
                     /locus_tag="PGT18_RS05995"
     CDS             complement(23341..23589)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="PGT18_RS05995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_004221184.1"
                     /transl_table=11
                     /translation="MVSAKASYYWRLRKVREAYLKTADHTQTFVEVPSSAVQPVNMAAE
                     YKIIALIRGRNNLTPEITEQASDSFLKTLLGVLGNAQ"
     gene            complement(23830..24672)
                     /locus_tag="PGT18_RS06000"
     CDS             complement(23830..24672)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221181.1"
                     /locus_tag="PGT18_RS06000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AraC family transcriptional regulator"
                     /protein_id="WP_004221181.1"
                     /transl_table=11
                     /translation="MSNTFFNIFPNENFIDLCMYQFGYEQCDPGHSFGPATRNHYLFHY
                     VLSGTGTLMANNSKGETRTYSVKSGQGFMIFPGQINTYIADEQLPWKYMWIEFDGLRVK
                     EALSVTDLCKDAPVYHSHSKELREKLADEMLYIVNHPQESSFHLIGHLYLFLDYLLQSA
                     KSTKLVSSGRMSDYYIKEAINYIEQNFQNNISIEDIAAVCGINRSYFGKIFRNSIGRSP
                     QEFLMNYRMVKATELLKLTSLSIADVGSAVGYENQLHFSRAFKNIYGVSPREWKNQNK"
ORIGIN
        1 acaggaatcc ttatgacatg cccacttaag acagcaacat gcggatgtca tatcacaggt
       61 aaataaagtt tggaagtgaa gcgtaatggc aaaatttgat gattttgatt tggatattat
      121 cgaaaaaaaa gtaagaagag atattatact tgcgagtatt acaagtcaac actctttttg
      181 tacacccaat tgcccaacag gatttctttg tccaccaaaa actcagctta catgcacatg
      241 taaattaaag gggcgataaa tctgttttag tgtacataca taaaaaagct ctattttgat
      301 aatagagctt tttttacgag caaatgagta ttttttacat gttgggtgat taattactaa
      361 tttttgttat tattagttag taaaaacata aaggagcaga taatggaaaa gatacttgtg
      421 attgacgatg acgaaaagat tttagaatta gtacaagaaa ttttgaaaaa tgaacattat
      481 atagtggaaa caagaagcta tgttgataat actaatattg gtgaatttga agggtttgac
      541 ttaatattat tggatattat gttaccgttt ttagatggtt atgaaatttt ggagagaata
      601 aaaaacataa taacatgtcc ggtgattttt ttatctgcta agtctagtga aggagcaaaa
      661 gtgaaaggcc tgatgagtgg agcagatgat tatattacca aaccttttag taacagagag
      721 ttagtagcca gagtgaaagt tgcattaaga agaaatgtga aaaatgagag taacgggatt
      781 ttaatcaatg gattggtgtt taatcaagat tcaaactcaa taaaattaga taatggtgaa
      841 attttattaa cgaaaaatga gtttcgcata tgtaaaattc ttgttcagaa tagcggaaag
      901 attttttcaa aagatgagct atatgaatac ttatatgact tagatgccaa tgcacaatta
      961 aggactataa cagagtttat ttactcaatc agaaaaaagt tcaaaacact tggtcttgat
     1021 ccaataaaaa ctatttgggg gataggttat aaatgggata taaaataata acgatgaaga
     1081 gacagttatt actgtacttg ataaaattgt ttgtgggaat cgtggttata ataatctgtt
     1141 ggttggcttt aactaatata tttgtggaat gcggagtgat aattccagca aattatagcg
     1201 aaactatttt ggaagagaat agaaagcgtt tagatgatat acaacagata acagataatg
     1261 atttaccata tggaagtaag tattcaatct ttgatttaga ttataattat gaacgtggga
     1321 ctatgaataa atcagatata gaagtaacta aaaaaatatt attagggaaa gaaagtaatc
     1381 ttcaagggaa ttatgtttac tcagtaattg ctagaacaga agaatattgc gttataaagt
     1441 ataatattaa agcacgtttt aactctgaca ataaaatttt caattatata gactatgaca
     1501 atttgtcata tattacaatg gtgttggtgt ttttactata tgtgtatatt atgacattac
     1561 acttagttag catctggaaa aataattttg aaaagataga gaaaattaca ctggaaatag
     1621 aaaaacagaa tctggatttc acttacgaag aaagtaagat aaaagaattt tctaatatta
     1681 ttacagcatt aattaagatg agggatgcat taaaagagtc attgtatcaa acttggaaga
     1741 tagaaaatga aaaaaatgag gaaatagcag cattggctca tgatataaaa ataccgctta
     1801 caattatcag tggaaataca gagcttttaa aatgttattc atctgatgag tacagtttat
     1861 ctcatctgca aagtatttgg ggagctgtgg gcaagatgga ggaatacgtt aatttattaa
     1921 ttaaatatgt gaaagctgat agaattgatt ttcacgaaaa ggaaagtatg ccctgcaaca
     1981 gtttttctca taaaatagtc tctgagatta aagagtatat cagcggatct gaagaaataa
     2041 ttaaatttaa tgttgaaaaa gtgaggggca ctattaagat agactacata tctttagaaa
     2101 gggcaatttt taacattatt gataacgcaa tagaatataa ggtatatggt gacaaaataa
     2161 tgtgttttat tggacaaaaa gatgattctt atatatttac catatgtaat gaaaaaggag
     2221 agtttagtag tcaggttctt gagaatggaa ctaaattatt ttttacttca aataataata
     2281 gaaattcaat tcactatggt attggcctag cttatgcgaa taaagttatt caatcttgtg
     2341 aaggggaatt ggaactatac aattctaaag agcatggtgc tgtggtaagc ataaaattac
     2401 cgatcacata gaggaaaagg ggggaaaccg taaatttggt catccccctt ttcttacttt
     2461 tacatatccg tatcactttc taatataata agtaactctt ctaatgatgc ttcgtctata
     2521 aattttcctt cgggagtata tgagtagcca tataagatgg ttccttcgtt ttgcaatgac
     2581 atatgtgcgt acactaactt gtcttttcca aatgcttcaa tgttttcctt aacagaaagt
     2641 agaagttctt cttttgtttt catggaaagg gcgcctttat ctttttctta agtatatcat
     2701 attttaggaa tatgttcaaa aaatcttgat atttttttga taatttcact ttatagttga
     2761 cttataacag attaggaggt agaaatgaaa aatttatact ataacactca aaagtttatg
     2821 tttcgtatac caaatgatgt agagagaaaa cttgacttta cggaagagga gataaaaaac
     2881 gcgtgtttag atgcaaagtt ccgtgaaaaa gtcagtatag caagtcctgc attagttgat
     2941 atgatggatt tgtacattaa aaatccggag aaattatctg agaagaagtt agtaaacttc
     3001 caaaattcaa ttatgaaata tttgattagg agcaaaaata gaactacacc atttgggctt
     3061 ttttcaggtg tcggtctggg aaaattcgga gatagtgata cttttgacgt atcaggggct
     3121 aaatatcaaa agaaagtcaa tattgattca gaatggcttt ttggatttat atctcagtta
     3181 gaaaagatta agtcacaaag acttaggttt aaaataaatg atgcatgtta tattaaagga
     3241 aatagagcta ttcttttata ttctactgaa aaggaaatag aagaaattag tgtaagagcg
     3301 actagggtct ttgaaattat ttatgagtca tgccaggaat tcaaagaata taaccagatt
     3361 gctagaatca ttgaagaaga gtatcctaat gtaccaaatg aaaaagttac tttttattta
     3421 aatgagctta tttcaaaaga aatactaatt tcagacctta ggccgtcttt aaattcaaga
     3481 gatcaaatag catatgtaat tgagcgatta agagaaagtg cgctttttga agaagcagga
     3541 aatattatag agataagcaa gatgtgtacc tcctacatga atttaccagt aggtgaaggg
     3601 ataactctgt acgataaaat agtatctaaa atgaagctac tgtattcttg ctcatcttac
     3661 ttacaagtag atacagtaat tgaaaatgct gtgtttgaaa taaaatcaac agttgcaaat
     3721 aagattaata ggttggcaag cttttttgtt tatataagca atgataagaa tgagagtcac
     3781 acttatttag atgaatatag gaataagttt attgaaaagt atggagttga cagagaagta
     3841 ccattattag aaatgttaga ttcaaatatt ggaataggag caccgacttc atatcttaat
     3901 ccccaaaatg atttttttga ggaagattct actaaaccta attacaattt aaggttaaaa
     3961 aattatttgc tgaataaata tgaatctgct attaccaata aaacatctat cactttagaa
     4021 caggacgaaa ttgaggggat tctaaaaaga gaaattaaaa cggacgaagt ccccatatcg
     4081 ttagagctgt atttccaact taagaagaaa aatgatgaat taaatctttg tttaggtcct
     4141 aattgtggtt cgtttgtagc tggtaaaact tttggtcgat tctccacaat ctccgatgaa
     4201 tttgcggata tgttggaaga tattaataag gaagaaagaa gattacgtga tgataatatt
     4261 gaaatgtgtg agattgggtt tcttcctgca ccggtcagaa atggaaatat tgttagaacc
     4321 cgcacattta gagaaaagaa aacggttatt tttacagcag cagataaagg aaccactgat
     4381 gttatcaata taaaagatat ttccataggg gtttttaatg agttgtttta cgcaagagat
     4441 tataaaacaa agaaattagt cgtctttgaa tctaataata tgtacaaccc gatgctaaat
     4501 cctaatatat taaggttttt acaggacata tcacatgaag ggaagagatc ttggtcagaa
     4561 tttccttgga catatatatt ctcagagttc cgccatgtac cagccataaa gtttgaggat
     4621 attgtaattg aaaatgaaaa atggaagtta aatcttagtg agatgcgatt agaaaagaaa
     4681 aattttgagg aatttaaatg caagttttta caacttataa aagataaaaa cataccggat
     4741 gatatttatt taacagaagc tgataacaga ataaagctgg acttaaaaaa agaattgtca
     4801 attaggatta tttttgatga atttaagaaa catggtagtc gtgatctgat tcttgaaaga
     4861 gctgaagctg gtgaaaatat tacttatagt ggtgaagggg gtcatacaac tgaaattgta
     4921 gttcctcttt tcagaaaaga aaaggaacta cagaatgttt atccggcgga gaaagtgatt
     4981 attgaaagaa agaaacattt agaattacct tttgaaaatt ggttatattt taacctatat
     5041 tgcaatagta atcgtgaaga tgaattgatt gcctttgata tcatggattt ctgcgaagag
     5101 cttaaaaaga aatatgatgt agattatttc tttatgcgtt atgtagatcc taaaccacat
     5161 gtacggttac ggataaaagg aacacaagaa gtattgttac agatatatcc gctaattatt
     5221 aaatggcagc accaattatt ggatgatggt ataattggtg atttaaaaat ttctatatat
     5281 gatagagaga ttgaacggta tggtggtttg catcttatgg atattgcaga gcaagtattt
     5341 tttatcgaca gctttattgt tgaaagtatc ctcagaatga agcgattagg ggtattagct
     5401 atggatcagg aggatatagc aattatctca atcattatgt atactcaggg attttatgaa
     5461 acctttgaag aacaaatgaa ttttctggca ataaattatc atacatcaga ctttatgtcg
     5521 gaatttaaaa agaaaaagca aagattagtt tcattatgcg ggtgtgagaa tgattggaaa
     5581 gaattattaa gtaatgaaga aggaacttct ctatataatt tattaaatat gagaacagtt
     5641 gtacttaata aatataggga tgaaatcaac aatataaacc aggatccgtt gtttaaaaat
     5701 ggtatagttg caagcgtaat tcatctacat tgtaatagaa taattggaac ggatcgcgaa
     5761 ctggagagaa aattaatggc attttctgaa agtataatgt atgctaagcg atacataatg
     5821 aagaggaatg aagatcatga ataataagaa agtagggttt aaagaagttt ggagggcttt
     5881 aaagcagata cctaaaacga taactataat tagaaaagtt gataaaaaag gatttacact
     5941 tatagtaatg ctaagtatga tagcgggggc ttttcctgta ataacattgg tattatcaca
     6001 ggaattaatt aactcaatag ttaagaaaag ctcaccatta cgatatacca tcattctatt
     6061 tacgctttat attatcgttt catttttagg aagtattctt ggagaagcaa aagattatat
     6121 tgaaagcaaa tttcaatatg ttcttcagta caaattgaat tacttgatta tggataagtg
     6181 tacaaaactg acattaaaag attttgagac gcctgccatg tatgataaaa ttgaaaaaat
     6241 caccggagaa gtggcatata agccatatca aattatgatt gcaattatag gaattattac
     6301 ttcggttgta acaatgctat cttcagcctt tttattattt gcatggaatc catatatatc
     6361 tatgctatta ctcgtagttc ctattatatc aatgctatat tacttaaaaa tagggcaaca
     6421 ggaatttgaa ataatgtgga atagagctaa agacgaacga aaaacatggt atctaactta
     6481 tttactgacg catgattttt cattcaaaga gatatcctta ttggatttga aagactattt
     6541 tcttaaaaag tattggagta ttagtgctct tttcattcgc caaaacacct ccatcctgaa
     6601 aagaaaaacg ttattcaata ttctatatga gttatctata caaattgtga gtgcaatcat
     6661 tataggagtg gctataatgt cagcatatgt tggtgagatt cttgttggta atgttgtgag
     6721 ttatattagg agtgtagcgt tagtacaaac aaattctcag gcaataatgt caaatattta
     6781 tacagtatat agtagtacat tgtatatgga tatgcttttt caatttttga attactgtgt
     6841 aaaagaagat agttcagaaa aaatagcgat gaaggaacca attaataaaa tagatattaa
     6901 agaactttca ttttcatata atggaaaaac aaaagtattg gacaaaatta atctgacatt
     6961 ggtacaaggc gaaaaggtcg ctcttgtagg tcctaacgga tcaggaaaaa gcactttttt
     7021 gaaaatttta gccgggctct ataacatcga agaaggggaa atattgataa atggtagatc
     7081 gttaaaagat ttggatatca gaagttatag atcaaagatg tctgttttat ttcaagattt
     7141 tgtaaaatat gagcttagtt taagggaaaa tatcggattt ggagatattc aaaatctaaa
     7201 caatgatgaa aaaatggttc aaatgcttca taagttacaa actaagtttt tgcaaacgga
     7261 taatggaagt tataatttgg atttgcagct aggaaattgg tttgaagatg gatgtcaatt
     7321 gtctcaaggg cagtggcaga aaatagcatt aggaagagtt tattttagag atgcatcttt
     7381 ttatatttta gatgagccaa atgctgcact tgatacagta tctgaaagag aagtttttga
     7441 aaaatttttt gaattatcaa aagataaaat tggcgttttt atttctcatc gccttaatgc
     7501 agcaaaaatg gcagataaaa ttatagttat ggatcagggc aaaattgttg gaataggcaa
     7561 acatgatgag ctactgcgaa actgtatgac atatcaaaag ctatataaag cggaatcata
     7621 tgaaagagag gacatgtagg aaaatggaat gcgatatttt aactatgacc aagaacattg
     7681 ctacaaattt aagtgattac gaaagaatgt taaaaataat ggggagaaaa gaaaaccaac
     7741 gaacttggga aggaatagaa tttgaagcat tttcaccact tactttgagt catggtcttc
     7801 cggggatttg tttattgtac ggaaaattaa tggagtgttg tccaaaagaa gacaaatggg
     7861 gacgatttgc tcataattat ttatcactaa tggtagaaga gctaaataaa aagggatttc
     7921 aaagtctgtc tatgttttca ggtgctgccg gtgtggctct atcagtagct agtgtttcca
     7981 atgatttttt aaactataac aaattactaa atacgttgaa tgactatatc ctaaatcggt
     8041 ttgaagagtc atacagtaat atagtaaaag aggaagggac acattcactc tattacgatg
     8101 taattgaagg gctgagcggt gttttgagct atctaggaat ttatagaaat caaagaaagt
     8161 attataatgt catcacaaga ggattggaga ttcttgtaaa tttgactaaa gacattgaga
     8221 ttcaaggtca agttgttccg ggttggtata ttccagttca aaaccaattt agtgaattag
     8281 aacaagatct ttatccaaaa ggcaatttca ataccagttt atctcatggt attgcaggac
     8341 cattaacaat tctttcagat atgatggcta atgggataat tgtggatgga caagaagaag
     8401 ctataataaa aataattaaa ttcctttttg attttaagtt gaatgatggt gaacgtgatt
     8461 tctggaaagg gcagatagat tttgatgaag tagtaaacaa aaaattaacc gatgaaaata
     8521 ttattcggcg agatgcatgg tgttatggaa atccgggaat atgttatgca attgtatcgg
     8581 ccggtaatgc tttagacaat cagcttttga ttgattacgg aatagaaaat ttgagactta
     8641 caatgaaaaa tattaaaggt attttttcac ctacattttg tcatggatat gctggtctat
     8701 accaagtatt aaattcggta gaaatgttgt tacaaaagga gtttttttct ttagaaaagg
     8761 aactcttgaa gaataaaata actgattttt ataatccgga ttatatatat ggatttaaaa
     8821 atatggagct tgacaataaa atggagaaaa taaaaccata tgatgtatgt ggactgttag
     8881 aaggaacgac cggcgtatgt ctctctcttc ttgaaggaga gaagaagagc gataatttat
     8941 ggaagaaagc ttttttattg gcataaaaag ataggatagg agtaaacggt gaatagtaag
     9001 taccaagatt ttcgctagtt ttcatgagat aattactttg aaaaatctaa gattttttag
     9061 aggaggaata atctcatgga taaaataact cataaagttc gatgtgaaca atggacaaat
     9121 atcatcaaag aatgtcttgc tagtggaatg cccaaaacga cttggtgccg agagcatggt
     9181 atttcaggta aatcattttt ttactggcaa cgaattctcc gagaagaagc atacctctct
     9241 acattagaga acaccttgac accagctgtt aaagaaaatt cagttccaac aactacagat
     9301 ttcgttgaaa tcaaaatgac tgatcacaca agttcatctg ccagtccttt taaaccggat
     9361 gttgctattc gaagagaatc tgtttcaatt gaaatatcaa atacagctac agaagagaga
     9421 acttctaaat aaggttgggg ggcttctaca tgcttaatgc tggcggattt gaaaaaattt
     9481 ttattgccac aggtttatcc tcacgatatt tttatcctca tctttttcat gatgcattaa
     9541 ttttagccag tttttcaata gaaatataag gataacctta atttccagcc tgaaatagcc
     9601 ctaaagcatc ttaggctgga aatttacatg ttattggcac cctgaatctg tacttcagtc
     9661 atacacttca tgatagagca gaataatgat atctccatac ctgactgcaa aatagtattc
     9721 cgttaacatt tgatcacacc tcttcagatt tactgtgtag gtactgcttg atttcttcat
     9781 agctccgtcc gctttgtgct atagcatcca atagcttttt ctgacgaagt gcatcccgct
     9841 tatctaacag atcttttaat gttgctgccg cagcatcata tcgatgtttt gctttcgcca
     9901 gatcttgctg tgctttttca attttagtat ccagtagttc tgcgtttatc tcacgtgcca
     9961 tatttaccgc ctttctttct gaatgtttga tttcttaagt gtattactga tctctccgac
    10021 ccgataagta atatttcctt cattcatccc aaaagccttt aagatttttt tctgctttgc
    10081 tgttacagca tgatccaacc ggtatacatt atccgtctgt cgtgtcattt caatcttctc
    10141 aagttctttg attactgctg gaactgtcat ataattctgt ttcctctcaa gtttttcatt
    10201 ttcttcctgt aatgcagtat aaattttgtt tcggattatc aatgcgataa attgtgtgaa
    10261 aatccgtcct tcaacgcctt cgtttgtatg tgaacgcatg ctcttgtttc caaggaatga
    10321 tttatcagag gcaaaaagtt tctcagatac atcacggctc ttataaagat gcagtgcttc
    10381 ctttgacttt tataaaatgc caatatatgg agaattatta ctcaaaccta gttcgcaaag
    10441 gcaagtgttg ctttgacgct tataataaca agaaaacgtt catagaataa tttataagta
    10501 aaacctactg tcgtaagcaa aataatgaca tgaacaatcg aagaaattac atttggatat
    10561 atggatgtat tttatgtggt atgttgaaat cataatattt acgaggcgat tgctgattgt
    10621 cgcatgtttg aaaaaaagtg attaagatgt gttaggtgtt atttggatcg ttatctctta
    10681 acttcctgta aaattaactc tcgtttccaa agaatggctt atttatgcgg attttgcgta
    10741 ttgtatcgtt aatgataaga gaattaatta cctctcgaga attaacatat aaaatactcg
    10801 caaatctact attcatgagg gttttgggca atcgagagtt aaattatcag gaagttaaga
    10861 ttatctattg attctcacgt caggtacaga aacaaaatct gataatctgt atgaaaaaaa
    10921 taaattttta ctgacaatta ccgatttccc atttaataat tctactatct ctgtataaaa
    10981 tataatgacg taattgatta ctctcattat ttgtattacg ccgcatgaat tagggtagca
    11041 tagaattaca atataattat gaggtaatca attatggaga gtagaatcaa acagctcagg
    11101 gagaaacgag gacttataca ggaaattctt gcagcagaat taggaataac tcagcaaatg
    11161 cttagtaaat atgagaggga tgtcacattg ataaaggtgg atattctcaa gaaaatagca
    11221 acgtatttca atgtaactac tgattatctt ttgggagttt cagatgtaaa aagggatttg
    11281 cagagacaga tgaaaatgaa tgaaacatta gatgagtatt atgacttaat tgagatatat
    11341 aaggatttag attcgtatga caaagaaatg atatggtcaa ttatgcagac ggtaaagaag
    11401 gtgggagaaa agcgaaaaaa ggacaataaa ctgatagaag agagttgatg tgaatgctga
    11461 gtatagcgat ttgtgatgat gatattcaaa caacagggca gatagaaatg atgattcaaa
    11521 aaatagcgaa aagaaatttt gctgatgtag atatagaagt attctggaat ggtgaaagcc
    11581 tggcagattc ggttgtagca ggggatggat acgatatcat ttatctagac atagagatga
    11641 acaacgagga tgggatttca acagctaaaa gaatcaggaa atatgataaa aatgttttga
    11701 ttatttatgt taccagtcat gaaaattata tgaaagaatc tttttcagtt cgaccatttc
    11761 agtttctggt aaagccagta agtgaaaaac aaatggaaac atgttttatg gcagcttatg
    11821 aagacataaa tagtggagat ttttactttc gttatagtta tcaacgtatg aatcataagg
    11881 tattgatccg agatatttta tactttgaga gcagtaaacg gaaggtgttt attgtgacta
    11941 gagaagaaac ttttgagttt tatgggaaat taaatgagat agagaacagt ctgaaggtat
    12001 gtaaaatctc ttttttacgt gttcatcagt cttttctcgt aaattataag catataaaag
    12061 gacagtccta tgattttgta gtaatggaca atgaaaaaaa gatttccatt agtgaagaca
    12121 gaagaaagct tatcagcgag cagtattgtt cgatggagga tacatattat gtggatagat
    12181 aaggctttgc cttacggagc agatatattg ctgagtgctt atgcagtata tctgttcttt
    12241 tattattttg atatattttt tgctcgaaaa aagagtaaaa aattatgggt ggctggattg
    12301 acggaatttg ttttgtggca gtttggaata tcgactatca ttagtcttcc tgcttatgta
    12361 aatattgcag ttacagtaat tgttacattg tttgcagtta cgatcactta tgagggtatg
    12421 tggtggaata agtgcgtgtt tgtgattaca tttaatgcaa tctggatgct tatggaaacc
    12481 ctgagtaatt atgtattgat gatttattgt gagcaatatg cttctgttca gtctatcgga
    12541 tcgtttgttt ctaaatcatt ttttcttgca gtgattattg cactgaagaa agtattcaca
    12601 gatgatgaga ttaaagaact gtcggttcga tacagcatta tgctcgtttt gattccgatg
    12661 gggagcatat acattatgaa caatgtattt atgcttggat ttacagtaaa tgataatcat
    12721 actcgtatta actcggcaat aacagtaatt attttgttgg gaatgaatat acttatattt
    12781 tacatttata tgaaattagc cgatgattta cgattaagac atatggcttc tgtatatgag
    12841 cagcagcttg aattatgtga acgacatcag caggaaaggg aaatttcaat acttcaatta
    12901 cgtgatgcca aacataatat gaaaaataat cttgtgtcca tccttgctta tgcagaaaac
    12961 aggcaacatg aaaagattat tggatttata aatgaaatta tggaagaaag tggaatggct
    13021 atagctgcca tttctaacag tggaaatata gtcattgatt cactgattgg gtattggtat
    13081 gtaaccgcaa agaagaaaga aatagatttt caaacggata tttgcattcc tatgatgcta
    13141 ccgtttaaag gcgcagatat atgtttgata cttggaaact tattggaaaa tgcagtagaa
    13201 gcagcacaaa aagtggatgg aaagagatat atagaaatca aaatgaaata tgataagaat
    13261 aatattttac tttcagtaac aaatagttat agtggaaaat tgctgaaaac aaaagatcac
    13321 cgattaaaat caacaaaaac tgatgctgaa aatcatggtg ttggactggc atctgtttac
    13381 cgggcagtcg caaaatattg tgggactatt ataattgatg actctgtgtt cgggcagttt
    13441 aagattcggg tattgttata cggaaaccag gaataattac ataagctctc gaaattatta
    13501 caacaaaaat atagactatt aaaaatgatg gtaaaataaa atgataaatt aattatgcgg
    13561 ctatgaggtc ttaatgtgga gaagatatta ttatttagcg atgatgaaaa attatttgag
    13621 attactaaac aagtaacaga aggaaaacag gaattaatat ggggcaatta tcagtgtctg
    13681 gaaacaaatg gatatccgtt tgcggatgtt gttataatgc attttgatga ggataaaatc
    13741 agaaaagata tacttcaacc aataataaag gtaaaaggaa gattagggca tttgactccg
    13801 atccttgctg ttatagaaaa tggaagtatg caggacattt tttcagttct taaaataggt
    13861 gtgtatgatt atatagaaac aatagatgac ttggagaaat atcagaaaaa aatacaagat
    13921 attattttat gggattggta tttgaaaaaa tataaaggtg gataaaaaag caatgatcag
    13981 tgtggccatt gtttttttga acaatagtat ctggaaaaaa ttacatgaat actcgttttt
    14041 tttacaagaa agaaaaagga taagtttttt atagtaggat gtaagaaaac aaatgaagga
    14101 ggacttctat tatgaaactt aaagatgtaa aaaaacagtc ggcaaaaatg ctggcaagtg
    14161 tgtcattaaa acttggcaaa atgtcagccg acagtgcgtg ctgttatatt tatcatcagc
    14221 cgaaaatgcc ggaggagttg aaaaatatga aacagtctta atatgaaaca actttcggaa
    14281 aagataacaa aatatgttat aaaaactggt gctgtttccc cggaattata tgtagtatat
    14341 caatatggct ttcagattgg acttgagatg ttatgctgtt ttgcaacttg ttttggcata
    14401 gcaatatata tgcatatgat tccggaattt gtagtattta ctgggatttt tatattattg
    14461 aggacatatg cagggggtgt acaccttaat agctttggtg catgctatat atgttcagtt
    14521 gctgtacaga cactagtaat gtattttaac aacaaataca aatttacagt aagtatagcg
    14581 tgggctctta tactaatgag tgcaatactt atattaaaag cagcaccagt ggagagtatt
    14641 aatcgagaac ttaacaatac tgaaaaagaa cattgcaaaa aggtaactat gaaaatttta
    14701 accggagtta ttgcatttgc catctgttgt acagttggtt taatacagga tatgctttct
    14761 ttgattgcat tgactacctt tataatttta atttctcagt atgttggtat tttgaaatat
    14821 aaaattgaaa atagtaagaa taagcggaga tgataaaatt attatttccg cttttttatt
    14881 acataaaaag aagggagaag attacctttt gaatattaga ttatttatat aaaaacgtcc
    14941 aatggatttc aatccgtaag gacgtttttt atatattacg aaaacttttt atagagtttt
    15001 tcttttaaac ccgaacaatg ggaagttctc aatcgggata agggtgaaag ggaaaaagac
    15061 ctttgctcat atcttgtggt cgggaaggag gtgaactcta tggagcaatc ttcttcctat
    15121 gatgagaaaa cagtcaggca tcaatttgat cgaaaatgta aattagcatt gaaaggcgaa
    15181 gtggtggatt atgaaagaca tttggcatat cttagaaaac atgaagtttt gttttcagaa
    15241 ttatcggaac gggaaataga gaatttttct ataatcaatg agtatgcatt ggaaaaaagc
    15301 tattttcgtg caggtggtta tgatgtggtg gtaaaagatg ctcttttggc agaagcattg
    15361 aaagcactga cggaaagaaa acgggatgtt attctcttgt cgtattttat ggaaatgaat
    15421 gatgctgata tagcaagaaa attgaatctt gttagaagta cggtacatga gcatcggaca
    15481 cgatcacttg aaatactaaa ggctatgctg gaggaaaatg taaatgaaaa cggaagaaaa
    15541 aagtgtaaat aataaaaaat tgataccgct ttgcgtgatt gaaagagcat tggaaggtga
    15601 tatccaggcg ataaataaag tattgaaaca ttatgaggga tacattgcaa aattatcaat
    15661 tagaaaaatg tatgacgaat atggaagtgt ttattatggt gtggatgaga cgcttcgcag
    15721 acgattggaa acaaaactga taaaaacaat tctgggattt aaaatggttc gctaaataat
    15781 gtataagagc atccctttct gctgataaga atatttagcg ttgcttgtac cttgacaatt
    15841 taatatttat cctgcaggac atatgaaaca gagaagctat acagcggata cgccaggact
    15901 gtctgcatta ttactttgcg acatcgtgac gcaaagtgga aatgttgtaa acgaagaaat
    15961 atcaaaggga tattgaagta aaggcacgag cgataaatat tggactgtga tcagaaagtg
    16021 ggatttttgc tgcgaagaga ctgtttctga taatgatact tccgggaaag ctgcccggtc
    16081 tgaaaagatg gtgaaatacc aatgaagctg ttacccgaca gctacctgca ggattatcta
    16141 taaaataaat aaggaaggga aaatgaacag tacaaaatta ttaagatata gtatgcagct
    16201 atccatgcta aagcaactta gaacattaaa acttatcaat gaaaatgaat atcgcttaat
    16261 agagaaaaaa ctaaaaaaag attatggtgt aatttccaat attactgctt gactagtaca
    16321 gagttgcgtt gtagaataat attaacacta cgataggaat acataagaaa ggaccggtga
    16381 aatggaagtt gaaatcatta aagcgaatag ttctggtgaa gatatttcta atggaagaag
    16441 gattgacaga ttaagagttg ccgcatattg tcgtgttagt actgatgatg aagatcagat
    16501 taaaagctat aattcaatgg tcaggtatta tactgatttg attaaaagta ataaacagtg
    16561 ggtatttgcc ggagtctttg cagataaagc tatcacagga acgaaaacag ataaacgtga
    16621 agaatttcag ttactgattc aggaatgcct ttcaggaaat attgatatgg taattgcaaa
    16681 gagtattcca cgatttgcaa gaaatacttt agacacatta aaatatgtaa gaatgctaag
    16741 agaaaggaat atagcagtat attttgaggt tgaaaaaatt aataccttaa aggatggcga
    16801 atttttactg actattttaa gttctgttgc acagcaagag gtggaaaata catctgcata
    16861 tgtaaaaaaa ggcttaaaga tgaaaatgaa acgaggagaa ctcgttggat ttcagggatg
    16921 cttaggttat gattatgatg tggtgactaa atcgctttct gttaatgaag aaggtgcgga
    16981 aacggttcga tacatatttg atagatatgt tgcaggagct ggaagtacga tgattgcaag
    17041 ggaattaaac gaacagggaa taccaaccat taagggaaat tcatggacct cttccagtgt
    17101 gatgggaatt attaataatg aaaaatataa aggagatatt ttattaggaa agacttttac
    17161 agtagatcca atatcaaaga gaaggttgga aaatcttggt gaagaagata ggttttatat
    17221 aaagaaccat catgaagcaa ttatatcaga ggaaactttt gcaagagccc aggaaatacg
    17281 agaacgtcgt aatggaggac gtaaaaaggg agttgcccct gggaaaagag aaaaattcag
    17341 tagacagtat gcatttagct gtatgttgga gtgtggattt tgtggtgcga atttatcaag
    17401 gcgaaggtgg catagcagct caaaatatac aaaaactatt tggcaatgcg tggaatcaac
    17461 gaagcatggt aaaaggtttt gcccagacag taaagggatt ccagagcagg ttatagagga
    17521 tgcttttatt gaatcttacc gcatgttatg tatggatcat aaagacgtgt tggaggaatt
    17581 tattaagaga gttgagaaaa ctttattgga agattcgata gaagataaaa ttgaaaagct
    17641 taatagaagc gtttataata ttcaatataa gagaaaaaag ttgctggaaa attatttaga
    17701 aggtgttgtg gctaaagaca tttatgagga aacagatatg ggatatgaaa agaaattgtc
    17761 tgaagcaaaa tcacagctga gtatgttgga acagcaatat gacaatgagg gaagcttaca
    17821 aagaagactt gcagagttta agaaggtatt gtcaaaaaat caagttttag aagaatttga
    17881 tagaggaata tttgaaagta ccatcgaaaa agttattgtc ggtggttacg atgaaaatgg
    17941 agagaaagat ccctataaaa ttattttcat atataaaaca ggattcaaaa atgaaattgg
    18001 aaacgcaaaa gaacgatttg ggaagaaatc aaaagcagta gaaaaagcga aagaaatgtg
    18061 ttcccatatc gtagacgagg taaaggatat gtgttcctat gttagtgaca acacatgtgg
    18121 agacggttgt tctgatgatg tattgtggtg ataagaggaa ataggacggt tataccataa
    18181 aatgttgtgt tttttgagcg taaatcgagc aaaaaatggg gcaaagaaaa ccaataaacc
    18241 acctgctatg caggtgtgtt cccagatagc tttagcttca agtaaaaaac ctcctgttgt
    18301 ataataagtg taggttcgcc aaccgcacaa aattatacaa agaaggttat cctaatggac
    18361 aaaaatagtt tagcacatac gaagtgggaa tgtaagtacc atattgtatt tgcaccaaaa
    18421 tatagaagaa aagttattta taaacagata cgagcagata ttggacatat cttaagtgaa
    18481 ctatgtaaga gaaaagggat tgaaataata gaagcagaag cgtgcccaga tcatatacat
    18541 atgtttgtaa gaatacctcc gaaatatgta tttgaaagac cggagcaatg cacagggaca
    18601 atgctatcca gcgattggta ccatcgcaaa ggaactacat ctgtcctctt ggtggcgtat
    18661 ggattatgtc actgcggcgt atccagaagg gatcacctga agaaatccta atagacagag
    18721 aaaaagaacc accttccgtg aaaggtggca gtctcttatc tgttgttcag catctcctga
    18781 attgctgtgc ggataacaga tttatcacgg tcgttcagtc gcatatactg acgcaaatga
    18841 atatcgatgc ttggacgctt ttcctcgttc tctaaagcca taaggaagcg ggcggtaatt
    18901 cccacttttt ccgcaacctc ttcctgtgtt cgatttgcat ctacacggta ttttttgaag
    18961 ataggcccga tgtaggaaat atccattttt tctgtttcag ccactatcag ttcacctcca
    19021 ataccatttt acagttcagg tatcgaggcg tgaacgttct gcgagttcgt gaataacacg
    19081 aaatatgaaa aaaggtgcca tataaagtaa caccatattt aacaaatgta aaaaaataaa
    19141 aagacaactt tgctattctg attttacaga agcaaagttg tcttttttat ttatgcatga
    19201 gtttaaaaaa agaatttcag atcttcttct actgtgctaa ttcccgcaat accaaagttc
    19261 tcgaccaaaa cttttgccac attggggctg aggaatgccg ggagagtggg accgagatgg
    19321 atattcttca cgccaagata cagaagcgcc agcagaacga tgacagcctt ctgctcatac
    19381 cacgcaatgt tgtaggcgat gggcaggtcg ttgatatcgt ccagaccgaa aacttccttg
    19441 agcttcaaag caatgacagc cagagaatac gaatcgttgc actgcccggc gtccagaaca
    19501 cgaggaatgc cgttgatgtc gcccaaaggc agtttgttgt atttgtattt cgcgcatccg
    19561 gctgtcaaga tcacagcatc tttcggcagt gccttggcaa attcggtata gtattcccgg
    19621 ctcttggcac ggccgtcaca gcccgccatc acaacgaact ttttgatcgc gccgcttttt
    19681 accgcatcga ccacttgatc cgccagagcc agaacctgcg cgtgtgcaaa tccgccgatc
    19741 agctctcctg tttcaatttc ggtaggtgta gcgcatcttt tggcgtgttc aatgataacg
    19801 gaaaaatcct tctgctcccc gatttcccct gaaatatggg tacatcccgg atatcctgct
    19861 gcgccagtag tgtagagtct gtctttgtag ctgtctttag gaggcacgat gcagttggtg
    19921 gtcatcagga taggaccgtt gaaggattca aattcctcct tctgcttcca ccacgcattg
    19981 ccgtagttgc cgataaagtt gggatacttt ttgaatgccg ggtaatagtg ggcagggagc
    20041 atctcggaat gggtataaac atccacgccg gttccctgtg tctgctccag cagcatttcc
    20101 aaatcccgca ggtcgtgacc ggaaaccaga ataccgggat ttgtcccaac gccaatattt
    20161 actttcgtaa tttcaggatt tccataggct tccgtgttgg ccttgtccag cagagccata
    20221 ccggatacgc cgtgttttcc ggtttccatt gtcagagcaa tcaattcttc tacgctgaga
    20281 ttgtcatcca gtgtagctgc cagcgcccgc tgaaggaagg catcgacctc ctcatcatcc
    20341 ttcagtagaa cgtttgcgtg tttggagtat gcggacaatc ccttcagacc gtaggtaatc
    20401 agttctcgca gagaacggat atcctcattt tcagtagaaa gaacaccgac agttgcggct
    20461 ttttcttccc aattgccaga gccattccat tttgccgctt tgggcagacc ggaaggatcg
    20521 gcaatctgcg ccaacagcac atctttttca atcagtgtgg cgcggattat tgcctcaatg
    20581 ttctctttgt caaaatttgc attggtgatg gtggtgaaca gattcagggt aatcaagtga
    20641 ttgatttccg cagatacctg cttcccttcc tgacgcagtg cagttgtaac agctgaaatc
    20701 ccttttgtga catagaccag cagatcctgc atagctgcca catcaggctt cttcccgcat
    20761 acaccggaca tggtgcaacc tttgcacccg gcggtttcct gacactgata gcaaaacatt
    20821 ttttgttcca taatagacct cctaaaaact tttaaaattt gaaatgatgt agttactttt
    20881 caatcaaaaa agtatataga tgcgcttgaa ttaatcttcc caagcgattg cagacacagg
    20941 acacgaatca attgcatcct gcacggcgct ttgattgtca tcgtttgctg gttggtatgc
    21001 ttccgccttc tcgtgctcat tcatacgaaa aacttcgttg cagatcccac agcaaagccc
    21061 gcagccaatg cagagattct gatctacttt tattttcata aaatcacttc cctctgtcaa
    21121 tttgtttttg tctgtcggat atattccaaa aactgaagaa ccgaatcaga ggttattcca
    21181 gaatctttcc atcaatcgag atagtgacta cctgccaggg aatgaatttt ccgcttgcct
    21241 gcaatgcttt tttagcagct aactccagtc cgccgcagca gggaacttcc atctgcacaa
    21301 tggtcacgct ctggatattg ttgttttgga taatctgcgt cagcttttcg ctgtaatcca
    21361 cgttgtccag cttgggacag ccaatcagcg taaccttccc acgcataaaa tcctcgtgca
    21421 ttctggcata ggcatatgcg ctgcaatctg ccgcaatcag caacttggct ccttcaaaat
    21481 agggggcacc tactgggacc agtttaattt ggcagggcca ctgcctcaac tgtaactcag
    21541 cctgcatcgg ggcagaagga gcagattcct gcgaatgctg gattcgctgc attcggctgc
    21601 ccgggcaacc cgcatgaaca ccaacggaag gagaattcat tctgttcttt tttctcatat
    21661 tttcctgcac agccttttcg tcataggcag cagcttcccg ttccacaaag gaaatcgcac
    21721 cggtggggca ggtgggtagg caatcgccca gaccatcgca ataatcgtcc cgcaggagtt
    21781 ttgccttccc gtttaccatg ccgatagcct cttcatggca ggcttcggcg caggccccac
    21841 atccattgca cttttcttcg tctatctgaa taattttccg aatcatgttt gcttcctcct
    21901 ttctcttgcg tttctggatt tagtatacta taatatgaaa tgaaagtctg ttgttataac
    21961 aacaaggagt gaaaaatatg aatttatcca atacacaact gtttcgagga ctggaagaag
    22021 cagagattac atctcttttg gattgcctga atgccacaac gcgtagtttt caaaaaggag
    22081 aggtaatcct ttcagaagga aacatcacgg aaaatatcgg gatcttgctg tccggcctgg
    22141 cggtgatctc ctgcaacgat atctggggca ataccagtat tttgggtcat gttgcgcccg
    22201 gctctgtatt cgccgaggtg tatgcctgca ttccggggga gccgatgttg gttacggtgt
    22261 ccgctgcgga ggatacatcc atattgttta tgaatgtggg gcgtgtcctg actacttgta
    22321 ccaatgcctg tccattccac acaagacttg tccgaaatct gctgactgtc tgcgctcaca
    22381 gaaaccttca gctttctcaa agaatccagc ataccagttc caaatccatt cgggggcggt
    22441 tgatgtccta tttttcggaa tgcgccaaac gcgccgggag caattctttc ctgatcccct
    22501 acaaccgcca acagttagct gattatctga atgtggaccg cagtaccatg tgtaacgagc
    22561 tttcaaaaat gcaaaaagac ggaattattg agtacagcaa gaatcaattt ttattaaaag
    22621 atagcttgat gtttcaatag agagtatgaa tgattcattg tatgttttct gatgatctat
    22681 tttatcaaaa aaatactttt aagtgtcctt caaaaatctc tcaagctgtt atatatcaat
    22741 atgttatacc tttccaatac atgggagtca taacttctgc aatctctggc agagtgacat
    22801 ctcgcaaggc caattatctt ccgtcgtatg ttccgaaaat cattgaactg gtgaattgct
    22861 atcatgatta tgacaagccc aaaaataaac tattgcaact tttgatggaa gccgatctaa
    22921 ttgtaaacaa agacacgccc atacctgttt cccttcaagt tccgtttgag caaggaggag
    22981 tgtttttatg tgttgggaaa ctatgctgct gatccgaagc agcactattt attgaaaccg
    23041 gatatctgtg taatggtgat cacttaaaaa aagcgactcc ctgcaattgg gatattccgg
    23101 acaacaagag gaacaattag aacatgaaaa atacgaatta agcgtctgtg tagactttta
    23161 tgtctatgca ggcgcttttt tgcgttccgc cagaacttta tgccgctgcc ggagtccgcc
    23221 gcttgtcctt cgttttcgca tgaattcgaa aaggaggaca agcctatggc aaagccatcg
    23281 atccctttgc gcagatccgt aaatccagtc gccagatata cttttttgaa cccggagcca
    23341 tcattgagca ttgccgagca ctcccaaaag tgtttttaga aatgaatcag atgcctgttc
    23401 ggtaatttcc ggagtgagat tgtttctgcc tctaatcaag gcaatgattt tatactccgc
    23461 tgccatattt accggctgga ctgcagaaga gggaacttct acaaatgttt gtgtatgatc
    23521 cgctgttttc agataagcct cacgaacttt ccggagtctc cagtaataac ttgcttttgc
    23581 tgacaccatt tatcgatttt cattccttgc ggacggtttt ggcagtccct gaccatgtca
    23641 gtccattcac tcaaacgggt ttgctgtgca actaaacttg tatgagattt cataactcac
    23701 ctcttgtagc aaagtcaaaa aagttgagta ctaaactatt tggactttgt agtgatgagt
    23761 ctattatctc acatcggatg attttgaaca ggtactcgct attttgcgtt tactttatcc
    23821 tatctattgt tacttgttct ggtttttcca ttctcgcgga gaaacaccat aaatattttt
    23881 aaaagcacgg gaaaaatgaa gctgattttc gtatccgaca gcagagccga catctgcaat
    23941 ggataaggaa gtcagcttta ataattccgt agcctttacc atgcggtagt tcataagaaa
    24001 ttcctgcggg gagcggccga tcgagttgcg gaagattttt ccgaaataac tccggttgat
    24061 gccgcatacc gcagcgatat cttcaatcga aatattattc tgaaaattct gttcaatata
    24121 attgattgct tcttttatgt aataatcact catgcgtccg ctggagacca gctttgtcga
    24181 ttttgctgac tgtagcagat agtctaaaaa cagatacaga tgcccgatga gatggaagga
    24241 agattcctgt ggatgattga caatataaag catttcatcg gcaagttttt cccgcaattc
    24301 tttggagtgg gaatgataaa cgggcgcgtc tttgcacaaa tctgtgacac ttaaagcctc
    24361 ttttacacgt aatccgtcaa attcaatcca catatatttc cacggaagct gttcatcggc
    24421 aatgtaagtg ttgatctggc cgggaaaaat cataaacccc tgtccgcttt ttacagaata
    24481 ggtccgtgtt tctcctttgg aattgtttgc catgagtgtt ccggttccgg aaagaacgta
    24541 gtgaaacagg tagtggttgc gtgttgcagg cccgaatgaa tggccgggat cacactgttc
    24601 atatccaaac tgatacatgc acagatcgat aaaattttca tttggaaaaa tattaaaaaa
    24661 tgtattgctc ataaaatact ccttatatac caaaatgcga catcaatatc tattatagca
    24721 taaaatattg catttcggta taaaactgaa aaaatataga tatttaagga tgcataaagg
    24781 tatgttataa tcagattatc aaaaaggtaa acggatatat tgcaaaaaaa ggagagagga
    24841 atatggcaaa aag
//
