LOCUS       NZ_CP115450            10855 bp    DNA     linear   CON 17-DEC-2024
DEFINITION  Kitasatospora cathayae strain HUAS 3-15 chromosome, complete genome.
ACCESSION   NZ_CP115450
VERSION     NZ_CP115450.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora cathayae
  ORGANISM  Kitasatospora cathayae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Kitasatospora.
REFERENCE   1  (bases 1 to 8860963)
  AUTHORS   Zheng,Y., Mo,P., Li,J., Zhou,Z., Huang,K., Luo,Y., Xie,P. and
            Wang,Y.
  TITLE     Kitasatospora cathayae sp. nov., a novel endophytic actinomycete
            isolated from the leaves of Cathaya argyrophylla
  JOURNAL   Int J Syst Evol Microbiol 74 (5) (2024)
   PUBMED   38809239
REFERENCE   2  (bases 1 to 8860963)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 155.15x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027627435.1-RS_2024_12_16
            Annotation Date                   :: 12/16/2024 15:42:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,288
            CDSs (total)                      :: 8,175
            Genes (coding)                    :: 7,919
            CDSs (with protein)               :: 7,919
            Genes (RNA)                       :: 113
            rRNAs                             :: 11, 11, 11 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 11, 11 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 79 of 256
            Pseudo Genes (incomplete)         :: 220 of 256
            Pseudo Genes (internal stop)      :: 9 of 256
            Pseudo Genes (multiple problems)  :: 51 of 256
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:37:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7454432
            Orig. end                         :: 7465287
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115450.1.
            Bacteria and source DNA available from Mo Ping.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10855
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="31"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10855
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10855
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5855](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5855
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            891..1118
                     /locus_tag="O1G21_RS33700"
                     /old_locus_tag="O1G21_33700"
     CDS             891..1118
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O1G21_RS33700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O1G21_33700"
                     /product="hypothetical protein"
                     /protein_id="WP_270149041.1"
                     /transl_table=11
                     /translation="MRAMWNAGDIPGRIIEIIIPSGFERYFRDLADLMVDSTTDRSAYL
                     ELAATYGLTYGHPDWLDDVVQRYGLTPPTH"
     gene            1707..2018
                     /locus_tag="O1G21_RS41865"
     CDS             1707..2018
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040332.4"
                     /locus_tag="O1G21_RS41865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trypco2 family protein"
                     /protein_id="WP_405000748.1"
                     /transl_table=11
                     /translation="MRPGPSPEPPETSGRTCRIIRDLRGRLGQAVADGAGRTIQFEPGE
                     VELEVSPALERSGEGRRGVFLGRGTGTGSRRSTLPTRLAPALVAAGPRWPLHRPMGRR"
     misc_feature    2256..2270
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.81"
                     /tool="antismash"
     gene            2512..4461
                     /locus_tag="O1G21_RS33705"
                     /old_locus_tag="O1G21_33705"
     CDS             2512..4461
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012744.5"
                     /locus_tag="O1G21_RS33705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_33705"
                     /product="glycosyltransferase"
                     /protein_id="WP_270149042.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.4e-09,
                     bitscore: 36.7, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNICFLTKYPPIQGGVSMHCYWAARGLAERGHRVFVVTNADEVEE
                     TFRIRIPETDRVGSGAYAPEFPESGGRVIVTSTQPPDRRELYYVPLGNPTVSRLAGLAT
                     ELIRAHDCEVIFSYYLEPYGVAAHLASRFTGVPYVFKHAGSDLHRLMRLPELRTTYTEV
                     LRGANRLISRGPSRTRLLEAGVDESAITSCAAFGLPTDLFTPVGPSRPLSQWIATGPEP
                     ARPVDSGLPVLGIYGKLGEFKGSFDLLHAMRRLFSDGFRFNLAAAVQGWQQDAFTRLAG
                     RLGLADHVHLIPFMPHWSIPEFIRSCTAVAFLERDFPIAAHTPTIPSEIIACGGCLIVS
                     TEVARKQLFRAAIRDRRNVVVTDPLRHDELAAALRYALEDGARAQDIGRRGHVELDYEP
                     TYESYVGELEQLLISVAAEPTGSSSPSRNPDTAGADPAGVAQALPGLYPRTQALLTADD
                     ERRLHRAAQVDGSHRDAADRRALALRVGQLLLSVLPPHAVVAREVCRYEYKIHEWTRDA
                     TKDKPTTPSVAELGWRARPDQARPRLVGQAEIVEFSCDVEPVLEALAAGREAPAGEGPL
                     KVLFHEATAPLRVSEPTAWVVELLRAGDLTVDQIAHLARERYDAIGPHSDRTSAAQCLD
                     VLEGLYWEGVVTFG"
     gene            4569..4778
                     /locus_tag="O1G21_RS33710"
                     /old_locus_tag="O1G21_33710"
     CDS             4569..4778
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O1G21_RS33710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O1G21_33710"
                     /product="hypothetical protein"
                     /protein_id="WP_270149043.1"
                     /transl_table=11
                     /translation="MSDQNKSQKIEVRGSVDAAKYHAAKALGGENTMFELRAMKPNRGS
                     NAAVANCIVCLICIVCAAGSPAAA"
     gene            5001..5855
                     /locus_tag="O1G21_RS33715"
                     /old_locus_tag="O1G21_33715"
     CDS             5001..5855
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="O1G21_RS33715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_33715"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_270149044.1"
                     /sec_met_domain="DUF692 (E-value: 2.9e-82, bitscore: 274.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREPSVLGVGLGYRQELREAILDAADSIDFLELITDQYIDMPPHK
                     AVEARELADRFPILLHGVDLSLGTDQPPDTEYVKKIQQLAERTDPEWVSDHLCFTQVPG
                     LNLGQLTPLAFTPETTEIAARNIRLVAQELDRPFAVENISYYFRVPWSTMSEAEFITEV
                     VTRADCHLLLDLTNVLNNATNLGFDAADFLDRIPLERVLQIHLAGGYWQDGVLLDTHSH
                     PVPAQVLELLLDFVPRMPALKAVMIERDQNFPPPAELLAELNGIRQALAPAWPPASPRG
                     GGR"
     gene            5864..6694
                     /locus_tag="O1G21_RS33720"
                     /old_locus_tag="O1G21_33720"
     CDS             5864..6694
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF017239.5"
                     /locus_tag="O1G21_RS33720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_33720"
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="WP_270149045.1"
                     /sec_met_domain="PF05402 (E-value: 7.1e-06, bitscore: 25.1,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSELQRQQALARFLTDPRARQAVLAEQPDPLPWADRELRDRLSEL
                     DQPRTSMYSHLLLVNRMTKVTDALPLVSWALGQRLWELAPEYNTCCPPRRPKKQDEALA
                     FAGFLRTKLGSDLNGPAWLADVLSYEVAVLRIRFFPGGPPAGALPAAPTFSREELHRVR
                     PRLTPATSLLALDHDLEEIGDCFERGEAPPRAQRRTMLLLLQATPEGSVHQDEVNPAVA
                     AFLRACSPERTLTEVIERVAREFQPVPPPETLREQCLALCAQLVQRGVLTLQAA"
     gene            6850..7425
                     /locus_tag="O1G21_RS33725"
                     /old_locus_tag="O1G21_33725"
     CDS             6850..7425
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O1G21_RS33725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O1G21_33725"
                     /product="hypothetical protein"
                     /protein_id="WP_270149046.1"
                     /transl_table=11
                     /translation="MERLSGLAMTVRRFIPGEDHTEQLNHVTQAMRDLREEERRGLFDY
                     PGGDDEYSEALEVVVDERRRLAITALGRGTPLAGNAGLVTSSLAMCDLIAASGVAFRAV
                     ACPSFMHDLLNQVRAIKEQGRFFMNRCPPRHCATDRSAPASLVGAERSVAGSLVGSDEH
                     VRGELVRDEHVRYCGRHELPEPRTAQSP"
     gene            7388..8080
                     /locus_tag="O1G21_RS33730"
                     /old_locus_tag="O1G21_33730"
     CDS             7388..8080
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987162.1"
                     /locus_tag="O1G21_RS33730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_33730"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_270149047.1"
                     /transl_table=11
                     /translation="MNSRNPAPRSRRERPAKPALSYEGIVATAVGVMRAEGLQRVTMRR
                     LAQELDTGPASLYVYVANTAELHAAILEELLGAVDLGPVSAAGDWRDRLAAVLGSYTRV
                     LFEYPGLAHSALVARPSGPNYLGLVEALLALLHEGGIPDAQAAWGVDVLLQVATATAAE
                     QSVRDRDIAAEDEHDALVDALRQVSAETYPRIAALGADLVSGAPEQRLAWIFRAVVNGV
                     QATPRQPD"
     gene            8165..9319
                     /locus_tag="O1G21_RS33735"
                     /old_locus_tag="O1G21_33735"
     CDS             8165..9319
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009191784.1"
                     /locus_tag="O1G21_RS33735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_33735"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_270149049.1"
                     /transl_table=11
                     /translation="MTTTTHHSIAIVGAGLGGLTLARVLYTAGVEAAVFDLDADRHART
                     QGGMLDIHEDSGQVALRAAGLHEDFLRLVMPGGEAMRVLDRHATVRFEESDDASSHTRP
                     EVDRGRLRDLLLDSLPDGMVRWGAKATATRTLADGRHEVALADGTVFTTDLLVGADGAW
                     SKVRPLVSSAVPAYTGVSVVEGDLLDADTRHPQAAAVVGGGMLFALGGGQGFLAHREWD
                     GSLHVYTAVRMSPDWLEGIDFTTPDAAKSAVLEQFADWHPSLRALVADADGPLVPRPIV
                     ALPVGHRWEHVPGVTLLGDAAHVMSPFAGEGANNAMQDAAGLARAILDHPGDTETALVR
                     YEQAMFPRAEAAAADSAANLALCFAADAPQGLVEQFASRQEQSA"
     gene            9348..10133
                     /locus_tag="O1G21_RS33740"
                     /old_locus_tag="O1G21_33740"
     CDS             9348..10133
                     /NRPS_PKS="Domain: Abhydrolase_1 (28-237). E-value:
                     2.2e-15. Score: 48.9. Matches aSDomain:
                     nrpspksdomains_O1G21_RS33740_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055778.1"
                     /locus_tag="O1G21_RS33740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_33740"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_270149051.1"
                     /sec_met_domain="PF00561 (E-value: 2.8e-10, bitscore: 39.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 3.2e-17, bitscore:
                     62.7, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="Thioesterase (E-value: 1.6e-07, bitscore:
                     30.6, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPTPTGRQVLLRPGVTLAVRESGHGAPVLLLHGGPGPDSLTPL
                     AEHLANRHRVLLPVHPGWDDTRRVPELTTVGDLAEAYLALIRQLDAHPTAVVGSSFGGW
                     IAAEMALRDTGRHLDRLVLMNAIGPAVPGHPLSVPGPPTGPSGNAAVRPTGDSESEAPR
                     RISSPAGLAALRTYTGPTMQDEGLLGRLKDAGRPVLVVWGEDDTVVSPDYGRTYAAAFP
                     DARFVPIPGGGHLPIREQPAATFAAIDGFLDALAAPNTP"
     aSDomain        9432..10058
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O1G21_RS33740_Abhydrolase_1.1"
                     /evalue="2.20E-15"
                     /label="O1G21_RS33740_Abhydrolase_1.1"
                     /locus_tag="O1G21_RS33740"
                     /protein_end="237"
                     /protein_start="28"
                     /score="48.9"
                     /tool="antismash"
                     /translation="PVLLLHGGPGPDSLTPLAEHLANRHRVLLPVHPGWDDTRRVPELT
                     TVGDLAEAYLALIRQLDAHPTAVVGSSFGGWIAAEMALRDTGRHLDRLVLMNAIGPAVP
                     GHPLSVPGPPTGPSGNAAVRPTGDSESEAPRRISSPAGLAALRTYTGPTMQDEGLLGRL
                     KDAGRPVLVVWGEDDTVVSPDYGRTYAAAFPDARFVPIPGGGHLPI"
     CDS_motif       9633..9680
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1G21_RS33740_0001"
                     /evalue="5.60E-05"
                     /label="NRPS-te1"
                     /locus_tag="O1G21_RS33740"
                     /protein_end="111"
                     /protein_start="95"
                     /score="15.7"
                     /tool="antismash"
                     /translation="VVGSSFGGWIAAEMAL"
     gene            10060..10506
                     /locus_tag="O1G21_RS41500"
     CDS             10060..10506
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015500.5"
                     /locus_tag="O1G21_RS41500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="WP_333493521.1"
                     /transl_table=11
                     /translation="MSSRPRPSPPSTDSSTRSQPRTRPDRDGWTQPARTDRIHLGAAEV
                     GLIPEPGFDFVVSEKCGATGECDRYASAYGGRVLDIEYGDASFVRVAKACDGWKSSMSV
                     GWCDRDLTAPGDSKVRLPEVRVTQLPCPLCAPSWRTRQRQGPVR"
ORIGIN
        1 cgggccggac ggcaacctgt ggttcaccga agtgctctcc ggaagcatcg gccagatcac
       61 caccggtggc acgatcaccg agtacgcggt tccctcgctt ctctcgctgc ccatcggcat
      121 caccgccggc ccggacggca acctgtggtt caccgacgcc ggagcggcgc agatcggaca
      181 ggtcaccacc gcgggcgtgt tcaccggcta cgatctcacg gatgccgtct tcccgttccc
      241 gtggggcatc accgccgggc cggacggcgc cctgtggttc gcggagaacg ccggcaacga
      301 catcggtcga atcacgacgt cggggacgat ctccgagctg gcgatcccga cctccggcgc
      361 gagcccgttg gaaatcacca ccggatccga cggcaacctc tggttcaccg agaactccgc
      421 gagccagatc ggacaggtag tcccctgacg ggaccggcaa ggtgaacagt cacccagccg
      481 ggccgctccc gctcccgcca tggactgggc gggagcggtc gccggcgggc cgaggtcggg
      541 aagctggacg acccgacccg gttctgatcc gatcccggga tcgggatgac cggatccggt
      601 ggcagtcgga tcgcaagtcc gatcagtgcg tgctacgagg cacgaaagtg ttccggagag
      661 gtacatatga ctatgcgcag ctggacggcc tgacaaccat caggttgcgt tgggacctcg
      721 accgcggcca tcgccgcagc tccgtgccgg agccgaaaag ttgacgcaca cccgcggccc
      781 catgcccccg gagctgctga gttccccgcg cagtagccgg accgcctgct ccagccggcc
      841 gtgaccgcgg tggactgtgg ggggagaccg ggcggccagc cagcggacag atgcgcgcga
      901 tgtggaacgc cggcgacatc cccggccgca tcatcgagat catcatcccc agcggattcg
      961 aacgctactt ccgcgacctc gccgacctga tggtcgacag caccaccgac cggtccgcct
     1021 acctcgaact ggccgcgacc tacgggctga cctacggcca ccccgactgg ctcgacgacg
     1081 tcgtccagcg ctatggcctc accccgccga cccactgacc cacactcaga acctcttcgt
     1141 ggcagagggc gtccggccgg agcgaagcag atcgagtaca ggatctaccg ggtcccgtag
     1201 accgctccat tcggctcggc aacggccaga ttccggtggt accaccggcg cagtgcacca
     1261 ccggccgccg gaccgagaac gtggaggcaa cgccgtcgcg cagggcccgc ccgaggcgac
     1321 cgacgtggcg gatccggtgg cggaggtgga gcgcagcctc gccgccgccg gcgaggggac
     1381 gtcggcgggg aaggcgcagg cggagccggc cggcgccgga tgggctgccg gcggttccgg
     1441 ggggagcccg gttgcactgc tgcgcagtcc cgggtgcgtc tcgcggaccg ccccgaacgc
     1501 gctggcctga cccgtcgcct gggcccgttt tcctcgcccg ggttccgggc gagcagggcg
     1561 acggcggcgc ggggatccga ccagatcgcc tccgccggcc gggcgccgac tccgccggta
     1621 gatcctcgaa caccgaacgt gccgctcatg cccgggagtt gccggccgtg acaggtccgt
     1681 catgcacgtg tcctcgaagt cctgcagtga ggccaggtcc atcacccgag ccaccggaga
     1741 ccagtggtcg aacttgtcgg atcatccgtg atctgcgggg ccggttgggg caggccgtgg
     1801 ccgacggcgc cggccggacg atccagttcg agccgggtga ggttgaactg gaggtctccc
     1861 cggcgctgga gcggtcgggg gaggggcgcc ggggtgtgtt tctggggcgt ggaactggga
     1921 ccggttcgag gagatcgaca ctgccgacga ggctcgcccc ggccctggtg gcggctggcc
     1981 cgcgctggcc tctgcatcga ccgatgggcc gacggtgacc ccaggagccc gagccgcacc
     2041 gcgttctgac acacccgaag cagccgcccc gggaaccggt gagaaccgtt ccgggcagca
     2101 ggtgagttgc agcgccgaca ccgaaccggc tggtgagaaa cgcccggaag cctggtgagt
     2161 ctccgcgctc gtggcccgcc gggtcccgtc acggcggaca gtactggcgt tgccacgctg
     2221 atggtcggtg ccggcacggt tgtgattctc ggcgtcattt tccgcgaatt ctcgtcgtcc
     2281 ggggcgcgtg aagtgcgtac acgctccggc gtatgcggac catccgccgg gctctcgtag
     2341 cgcagatata ttatcgtctt tagctcttgt gagccattta cgttcgcccg gatctccggt
     2401 tcactgtgag agacggcttc tgccgcgcca ccggtaccca ttccaaggcc tgcgaccggt
     2461 aaggcgccct caacctaccc gatagatctc ggcatcgctt ggggtggcgg catgaacatc
     2521 tgttttctca cgaagtaccc gccgatccag ggcggggtca gcatgcactg ctactgggcc
     2581 gcccgcggcc tggccgagcg gggacaccgc gtcttcgtgg tcacgaacgc cgacgaggtg
     2641 gaggagacct tccggatccg gatacccgag accgaccggg tcgggagtgg cgcctacgca
     2701 ccggagttcc ctgaaagcgg cgggcgcgtg atcgtcacca gcacgcagcc tcccgatcgg
     2761 cgcgagctct actacgtccc gctcggcaat ccgaccgtca gccggctggc aggcctggca
     2821 accgagctta tccgcgcgca cgactgtgaa gtgatcttct cgtactacct ggagccgtac
     2881 ggagtcgcgg cccacctggc aagccggttc accggggtgc cgtacgtctt caagcacgcg
     2941 ggcagcgacc tgcaccggct catgcggcta ccggagctgc ggaccaccta caccgaagtc
     3001 ctgcgcggcg cgaaccgcct gatctcccgg gggccctcgc gcacccggct gctggaggcg
     3061 ggggtggacg agagtgcgat cacttcgtgc gccgccttcg ggttgcccac cgacctcttc
     3121 accccggtcg gcccgtcccg tccgctgtcc cagtggatcg cgaccggccc ggagccggcc
     3181 cggccggtcg acagcggact gcccgtcctc gggatctacg gcaaactcgg cgagttcaaa
     3241 gggtcgttcg acctgctcca cgccatgcgg cggttgttct cggacggctt cagattcaac
     3301 ctggcggctg ccgtacaggg atggcaacag gacgccttta cccggctggc gggccggctc
     3361 ggactcgccg accacgtcca cctcatcccg ttcatgccgc actggtccat ccctgagttc
     3421 atccgcagtt gcaccgccgt cgccttcctg gaacgagact tcccgatcgc ggcgcacact
     3481 ccgaccatcc catcggagat catcgcctgc ggcggatgcc tgatcgtgtc caccgaggtc
     3541 gcgcgaaagc agctcttccg ggccgcgatc cgggaccgcc gcaatgtcgt ggtcaccgat
     3601 ccgctccgcc acgacgaact cgcggcggcc ctgcgctacg cgctcgagga cggcgcgcgg
     3661 gcccaggaca tcggccgccg gggacacgtg gaactggact acgaaccgac ctatgagtcc
     3721 tacgtcgggg aactcgagca actgctcatc agcgttgccg cggagccgac cggctcatcc
     3781 tccccgagcc gaaaccccga caccgccggt gccgacccgg ccggggtggc gcaagccctg
     3841 ccgggcctct acccgcgcac ccaggccttg ctgacggccg acgacgagcg gcggctgcac
     3901 cgggcggcac aggtcgacgg cagccatcgc gacgcggcgg accggcgcgc cctcgcgttg
     3961 agggtcggcc aactgctcct cagtgtcctg ccgccgcacg ccgtcgtggc ccgcgaggtg
     4021 tgccggtacg agtacaagat ccatgagtgg acgcgcgacg cgaccaagga caagccgacc
     4081 accccgtcgg tagcggaact cggctggcgc gcacggcccg accaggcgcg gccacgactc
     4141 gtgggccagg cggagatcgt cgagttcagc tgcgatgtgg aaccggtgct cgaggcgctg
     4201 gccgccgggc gcgaggcgcc ggccggagag gggccgctga aggtcctgtt ccacgaggcg
     4261 acggcgccac tgcgggtgag cgagcccacc gcgtgggtcg tcgagctgct ccgcgccggg
     4321 gacctgaccg tcgatcagat cgcccacctg gcgcgcgaac ggtacgacgc gatcggtccg
     4381 cactccgacc ggacgtcggc ggcacagtgc ctcgacgtcc tcgaaggcct ctactgggag
     4441 ggggtggtca ccttcggctg acgcagtggc gcacaccggc cgtgcccgcg gcgcgcgccg
     4501 gggagaacgg cagagcccgt ccgtacaccg gacgatcgcg aacgacgaaa ccatgaaagg
     4561 ggaatgacat gagcgaccag aacaagagcc agaagatcga ggtgcgaggc agtgtcgacg
     4621 ccgcgaagta ccacgcggcc aaggcgctcg gcggcgagaa cacgatgttc gagctgcgtg
     4681 cgatgaagcc gaaccgcgga tccaacgcgg cggtggccaa ctgcatcgtg tgcctgatct
     4741 gcatcgtgtg cgcggccggt tcgcccgcag cggcgtgagc cgtcccgatc gcgacgacct
     4801 ggtcggcgcg tgagggaaca ccggcgacat agggcggcga aggtgatccg ctgctttgcg
     4861 gcggcggggt cccgggccga gaacgcgccc ccaaccgggc gcttcgagag ccacgcgggc
     4921 accttcgccg gcccgacagg gcggccccgg gctgccacca cgtcacttga cgaccccgaa
     4981 ccggcaggag tgatgcgacc atgcgcgaac cgtccgttct cggagtcggg ctcggctacc
     5041 gacaggagct gcgggaggca atcctcgacg cggccgactc gatcgacttc cttgagctga
     5101 tcaccgacca atacatcgac atgccaccgc acaaggcggt ggaggcccgc gaactggccg
     5161 acagattccc gatcctgctg cacggcgtcg acctgtcact gggcacggac cagccccccg
     5221 acaccgagta cgtcaagaag atccaacagc tggccgagcg gaccgacccc gagtgggtca
     5281 gcgaccacct ctgcttcacc caggtccccg gcctcaacct cggacagctg accccgctcg
     5341 cgttcacccc ggagaccacc gagatcgccg ctcggaacat acgcctggtc gcccaggagc
     5401 tcgaccggcc cttcgcagtg gagaacatct cgtactactt ccgtgtgccg tggtccacca
     5461 tgagcgaggc agagttcatc accgaagtgg tcacccgggc cgactgccac ctgctgctcg
     5521 acctgaccaa cgtgctcaac aacgcgacca acctggggtt cgacgccgcc gacttccttg
     5581 accggatccc cttggagcgc gtgctgcaga tccacctcgc cggcggctac tggcaggacg
     5641 gcgtgctcct ggacacccac agccaccccg tcccggccca ggtcctcgaa ctgctgctcg
     5701 acttcgtccc gcgcatgccc gccctcaagg ccgtgatgat cgaacgcgac cagaacttcc
     5761 caccgcctgc agaactgctt gccgaactca acggcatacg acaggccctg gcgcccgcct
     5821 ggccgccggc ctcgccacgc ggaggcggca ggtgagcgag cacatgagtg aacttcagcg
     5881 tcagcaggca ctggcgcgct tcctgaccga cccccgggcc cgacaggccg tgctggccga
     5941 gcaaccggac ccactcccgt gggccgaccg cgaactgagg gatcggctga gcgaactgga
     6001 ccagccgcgc acctcgatgt actcccacct gctcctggtc aaccgcatga cgaaggtcac
     6061 cgatgcgctc ccgctggtca gctgggccct cgggcagcgc ctgtgggagc tggcgccgga
     6121 gtacaacacg tgctgcccgc cccgccggcc gaagaagcag gacgaggcct tggcattcgc
     6181 cggcttcctc cgcacgaagc tggggtccga cctcaacgga ccggcgtggc tggccgacgt
     6241 gctgagctac gaggtggcgg tccttcggat ccgtttcttc cctggcggcc cgcccgccgg
     6301 cgccctgccg gcggcgccga cgttctcgcg ggaggagctc catcgggtac ggccccggct
     6361 cacccccgcc accagtctgc tggccctgga ccacgacctg gaggagatcg gcgactgctt
     6421 cgagcgcggc gaggcgccgc cccgagcgca gcggcgcacg atgcttctgc tgctgcaggc
     6481 cacacccgag ggcagcgttc accaggacga ggtgaacccg gctgttgcag ccttcctcag
     6541 ggcctgctcc ccggagcgca ccctgaccga ggtgatcgag cgggtggccc gggagttcca
     6601 gccggtcccg ccgccggaaa cgctccggga acagtgcctg gcgctctgcg cccagctggt
     6661 acagcgcgga gtgctcacgc tgcaggcggc ctgaacccgc gcgggccgag cgtcccgggg
     6721 tcgggtcccg ccgatccgac gaggcatctg gctccgggct ccgtgggcca ccgaggtcgc
     6781 cgtccccacc gaccttgcaa gggtcagtcc gtcaagggtg agcgggtcac cgctgcgatc
     6841 gcggccctgg tggagcgctt gtccgggctg gcgatgacgg tgcgcaggtt catccccggc
     6901 gaggaccaca ccgagcagct caaccacgtc acccaggcca tgcgcgacct gcgcgaggag
     6961 gaacgtcgag gcctgttcga ctaccccggc ggcgacgacg aatactccga agccctcgaa
     7021 gtcgtcgtcg acgaacgccg ccgccttgcc atcaccgcgc tcgggcgcgg aacgccgctg
     7081 gccggcaacg ccggcctggt gacctcgtcc ctggcgatgt gcgacctgat cgccgcatcg
     7141 ggtgtggcct tcagggcggt ggcctgcccg tccttcatgc acgacctgct caaccaggtc
     7201 cgtgcgatca aggaacaggg caggttcttc atgaaccgct gtccaccacg tcactgcgcc
     7261 actgatcgct ctgcgccggc atcactcgtc ggcgcagagc gatcagtggc cggaagcctc
     7321 gtgggatctg acgaacatgt tcgtggcgag cttgttcgtg acgaacatgt tcgttactgt
     7381 gggcggcatg aactcccgga accccgcacc gcgcagtcgc cgtgagcggc ccgccaagcc
     7441 cgccctgagc tatgagggaa tcgtcgccac cgccgtcggc gtgatgcggg ccgaggggct
     7501 gcagcgggtc acgatgcggc gcctggccca ggagctcgat accggtccgg cctcgctgta
     7561 cgtgtacgtg gccaataccg cggagctcca cgcggcgatc ctggaagagc tgctcggcgc
     7621 ggtcgacctc ggtccggtca gtgcggccgg cgactggcgc gaccggctcg cggcggtcct
     7681 cggctcctac acccgggtgc tgttcgagta ccccggcctc gcgcactcgg cactggtggc
     7741 ccggccctcc gggccgaact acctggggct ggtcgaggcg ctcctcgcgc tcctgcacga
     7801 gggcgggatt cccgacgcac aggccgcctg gggtgtggac gtcctgctcc aggtggccac
     7861 cgccaccgcc gccgagcagt ccgtgcggga ccgcgacatc gccgccgagg acgagcacga
     7921 cgcgctggtg gacgcgctgc gacaggtcag cgctgagacg tacccgcgga tcgcggccct
     7981 gggtgccgac ctggtctccg gagcgcctga gcagcgcctg gcctggatct tccgcgcggt
     8041 tgtcaacggt gtgcaggcca ccccccggca gccggactga tcgcagccgg caccgcaccg
     8101 ggtcatcccg gcaccgggtc atcccgttcc tggtcgtcga atcccgtcga atcgaggtac
     8161 caccttgacc accacgacgc accactccat agccatcgtc ggcgcaggcc tcggcggcct
     8221 caccctcgcc cgcgtcctgt acaccgccgg agtcgaggcg gccgtcttcg acctggacgc
     8281 cgaccggcac gcccgcaccc agggcggcat gctcgacatc cacgaggact ccggccaggt
     8341 cgccttgcgc gcggccggac tgcacgagga cttccttcgg ctcgtgatgc ccggcgggga
     8401 ggccatgcgg gtcctggacc ggcacgccac cgtccgcttc gaggagagcg acgacgcctc
     8461 ctcgcacacc cgacccgagg tcgaccgcgg ccgactgcgt gacctgctcc tcgactccct
     8521 gcccgacggc atggtccgct ggggcgcgaa ggccaccgcg acccgcacgc tcgcggacgg
     8581 ccgccacgag gtggcgctcg ccgacggcac cgtgttcacc accgacctgc tggtgggtgc
     8641 cgacggtgcc tggtcgaagg tccgcccgct ggtgtccagc gcggtgcccg cctacacggg
     8701 ggtgtccgtc gtcgagggcg acctgctcga cgccgacacc cgtcacccgc aggcggccgc
     8761 cgtcgtcggc ggcggcatgc tcttcgcgct cggtggcggc cagggcttcc tcgcccaccg
     8821 tgagtgggac ggcagcctcc acgtctacac cgccgtcagg atgtcgcccg attggctgga
     8881 gggcatcgac ttcaccaccc ccgacgcggc gaagagcgcg gtgctggagc agttcgccga
     8941 ctggcatccc agtctgcggg cgctggtcgc cgacgccgac ggcccgctcg tgccgcggcc
     9001 gatcgtcgcc ctgcccgtcg gtcaccgctg ggagcacgtg cccggcgtca cgctgctggg
     9061 cgatgccgcc cacgtcatgt ccccgttcgc cggcgagggc gccaacaacg ccatgcagga
     9121 cgccgccgga ctcgcccggg cgatcctcga ccaccccggc gacaccgaga ccgcgctcgt
     9181 ccgctacgag caggcgatgt tcccccgcgc cgaggccgcc gccgccgact ccgccgccaa
     9241 cctcgccctg tgcttcgccg ccgacgcgcc ccagggcctc gtcgagcagt tcgcctcccg
     9301 ccaggaacag tccgcgtgaa ccgcccgacc agcgaggagc tcaccacatg accaccccga
     9361 ccccgaccgg ccggcaggtg ctcctgcgcc ccggtgtcac cctggccgta cgcgagagcg
     9421 ggcacggcgc ccccgtcctg ctcctgcacg gcggacccgg ccccgacagc ctcaccccac
     9481 tcgccgagca cctcgccaac cgccaccgcg tcctgcttcc cgtccacccg ggctgggacg
     9541 acacccgccg cgtccccgag ctcaccaccg tgggcgacct cgccgaggcg tacctggcac
     9601 tgatccgcca gctcgacgca catccgacgg ccgtcgtcgg cagttccttc ggcggctgga
     9661 tcgccgccga gatggccctg cgcgacaccg gccgacacct cgaccggctc gtcctcatga
     9721 acgccatcgg ccctgccgtg cccggccacc cgctgtccgt ccccggccca ccgaccggac
     9781 ccagcggcaa cgcggcagtg cggcccaccg gtgacagcga gagcgaggca ccgcgccgaa
     9841 tctcctcgcc cgccggcctg gcggcgctgc gcacgtacac ggggcccacg atgcaggacg
     9901 agggcctgct cggccgcctc aaggacgcag gccggcccgt gctggtcgtg tggggcgagg
     9961 acgacacggt cgtcagcccc gactatggcc gcacctacgc cgccgccttc cccgacgcac
    10021 gcttcgtgcc catccccggc gggggccacc tgcccatccg tgagcagccg gccgcgacct
    10081 tcgccgccat cgacggattc ctcgacgcgc tcgcagcccc gaacacgccc tgaccgggac
    10141 gggtggaccc aacctgctcg gacggatcga atccacctgg gagcagcgga agttggcctc
    10201 atccccgagc ccggcttcga cttcgtcgtg tccgagaagt gcggcgccac cggggagtgc
    10261 gatcggtacg catcggcgta cggcggccgg gtcctggaca tcgagtacgg cgacgcctcc
    10321 ttcgtcaggg tcgccaaggc gtgcgacggc tggaagtcgt cgatgtccgt gggctggtgc
    10381 gatcgcgacc tcaccgcccc cggcgactcg aaagtgcgtc taccggaagt gcgcgtgacg
    10441 cagctaccgt gcccgctgtg tgcaccttcc tggcgaacgc gccagcgaca aggtcccgtt
    10501 cggtgacgcg gcgagcagcc ggttgccctg gggcgggcac agtgcccatt cggtggcagc
    10561 atcgcactgt ccggcggatc gccaggctcc aggccatgag caaggccccc cgccttgacg
    10621 gtccgggcct gcctgacccg tcgggagccc ggctgtggac ccggcgagtt ccctcacgca
    10681 gacgaggaag agtgcaccgc cccatgttcg gaccgaagcg tggcactgat ggaacatcag
    10741 tgccacctca acgacgagga gccctgacgg tgacgaagaa gcgcggctcc gacgggacgt
    10801 tcgccttcga ggtcctggaa tcaggcggcg cgcagctcgg gttcgacctc ttccg
//
