LOCUS       NZ_CP115450            10798 bp    DNA     linear   CON 17-DEC-2024
DEFINITION  Kitasatospora cathayae strain HUAS 3-15 chromosome, complete genome.
ACCESSION   NZ_CP115450
VERSION     NZ_CP115450.1
KEYWORDS    RefSeq.
SOURCE      Kitasatospora cathayae
  ORGANISM  Kitasatospora cathayae
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Kitasatospora.
REFERENCE   1  (bases 1 to 8860963)
  AUTHORS   Zheng,Y., Mo,P., Li,J., Zhou,Z., Huang,K., Luo,Y., Xie,P. and
            Wang,Y.
  TITLE     Kitasatospora cathayae sp. nov., a novel endophytic actinomycete
            isolated from the leaves of Cathaya argyrophylla
  JOURNAL   Int J Syst Evol Microbiol 74 (5) (2024)
   PUBMED   38809239
REFERENCE   2  (bases 1 to 8860963)
  AUTHORS   Mo,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) College of Life and Environment Science,
            Hunan University of Arts and Science, No. 3150 Dongting Avenue,
            Changde, Hunan 415000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 155.15x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_027627435.1-RS_2024_12_16
            Annotation Date                   :: 12/16/2024 15:42:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,288
            CDSs (total)                      :: 8,175
            Genes (coding)                    :: 7,919
            CDSs (with protein)               :: 7,919
            Genes (RNA)                       :: 113
            rRNAs                             :: 11, 11, 11 (5S, 16S, 23S)
            complete rRNAs                    :: 11, 11, 11 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 79 of 256
            Pseudo Genes (incomplete)         :: 220 of 256
            Pseudo Genes (internal stop)      :: 9 of 256
            Pseudo Genes (multiple problems)  :: 51 of 256
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:37:27
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6678202
            Orig. end                         :: 6689000
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115450.1.
            Bacteria and source DNA available from Mo Ping.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="25"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5798
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            385..957
                     /locus_tag="O1G21_RS30335"
                     /old_locus_tag="O1G21_30335"
     CDS             385..957
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356942.1"
                     /locus_tag="O1G21_RS30335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30335"
                     /product="DoxX family protein"
                     /protein_id="WP_270148159.1"
                     /transl_table=11
                     /translation="MPSSSPSTTAATSPTARRARFGSGSAATSPHAYDAGLLLLRLVLG
                     LTMAAHGSQKLFGWFGGGGIDGTGQFFTMSGYPAGNAMAVVAGLTEILGGLGLAVGLLT
                     PLAGAAVVGTMVNAIAVTWGGGFFAPKGNEYELLLTAGAAALALTGPGRYAVDRFLPVL
                     KAHRLAYGVAAVLVGLATAGVILLLRK"
     gene            complement(1057..1818)
                     /locus_tag="O1G21_RS30340"
                     /old_locus_tag="O1G21_30340"
     CDS             complement(1057..1818)
                     /EC_number="3.1.3.16"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016572395.1"
                     /locus_tag="O1G21_RS30340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30340"
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_270148161.1"
                     /transl_table=11
                     /translation="MAPYIAVSALSHEGLVREHNEDSLAVGPWTLCATVTTNPQTLLFP
                     LGPPCVVAVADGLGGHPGGEVASALVARRLAATGGGLDGEEAVREELQECNRAVHAAAA
                     GEPELAAMGTTVAGVVLLPGQAVVFNVGDSRVYRVSGDGLDRVSVDDSPPLPPGRRTTS
                     VVTQTLGGSFGLTVVEPHVRVSPLAAGDRYLVCSDGLTDPVTEEELAELLGRSYDTSER
                     ADGKAAFELWKAAIEAGGPDNITLALVRIGE"
     gene            1993..3093
                     /locus_tag="O1G21_RS30345"
                     /old_locus_tag="O1G21_30345"
     CDS             1993..3093
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1251: luciferase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895843.1"
                     /locus_tag="O1G21_RS30345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30345"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_270148162.1"
                     /transl_table=11
                     /translation="MQFGIFSVGDVTPDPTTGRTPTEHERIKAMVAIARKAEEVGLDVF
                     ATGEHHNPPFVPSSPTTMLGYIAAQTERLILSTATTLITTNDPVKIAEDFAMLQHLADG
                     RVDLMMGRGNTGPVYPWFGKDIRQGIPLAIENYALLHQLWREDVVDWQGRFRTPLQSFT
                     STPRPLDDVPPFVWHGSIRSPEIAEQAAYYGDGFFANNIFWPKEHFQKLIDLYRERYAH
                     YGHGTPEQAIVGLGGQVFMRKNSQDAVREFRPYFDNAPVYGHGPSLEEFTEQTPLTVGS
                     PQEVIEKTLSFRESFGHYQRQLFLMDHAGLPLKTVLEQLDLLGEEVVPVLREEFAKGRP
                     AEVPDAPTHAALVAARDAREKEEVSK"
     gene            3090..3716
                     /locus_tag="O1G21_RS30350"
                     /old_locus_tag="O1G21_30350"
     CDS             3090..3716
                     /EC_number="1.5.1.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887982.1"
                     /locus_tag="O1G21_RS30350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30350"
                     /product="FMN reductase"
                     /protein_id="WP_270148163.1"
                     /transl_table=11
                     /translation="MTAFKLAVVTAGLSKPSSTRLLADRLAEATVRRLAREGREAEVTV
                     IELRDLAVDIANDFVTGFPAPALRGAIETVTEADAVIAVTPIFTASYSGLFKSFFDVID
                     HDALTGKPVLIAATGGTPRHSLALEHAVRPLFGYLRAVVLPTAVYAATDDWGTAGDALT
                     DGLPGRIDRAAGELAALLAGRGQTARAALPEQVVPFAEQLAALRV"
     gene            3924..4943
                     /locus_tag="O1G21_RS30355"
                     /old_locus_tag="O1G21_30355"
     CDS             3924..4943
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612844.1"
                     /locus_tag="O1G21_RS30355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30355"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_270148164.1"
                     /transl_table=11
                     /translation="MQPEPQAVLSPLTGAAVFLVLTVEPGGEEAVRDLLPDLAGLRRSV
                     GFRAPDGALTCVTGIGSALWDRLFDGPRPADLHPFQELAGPRHRAPATPGDLLLHLRAE
                     RMDLCFELAGQLTTRLAGAATVVDSAHGFRYFDDRDLLGFVDGTENPEGRAAVDAALVG
                     AEDPDFAGGSYVMVQKYLHDLDKWNALSVEQQEGAVGRTKLTDIELDDDVKPADSHVAL
                     TVVTGPDGEERKIVRYNMAFGSFERGGEFGTYFIGYARDPGVTEEMLRNMFLGDPPGTT
                     DRLLDFSTAVTGSLFYVPSADFLDAPPPAPAAPAAPAGPAAEARADGSLGIGSLKPTS"
     gene            5001..5798
                     /locus_tag="O1G21_RS30360"
                     /old_locus_tag="O1G21_30360"
     CDS             5001..5798
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834463.1"
                     /locus_tag="O1G21_RS30360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30360"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_270148166.1"
                     /sec_met_domain="Linocin_M18 (E-value: 6.1e-94, bitscore:
                     312.7, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPVSAAAWEEIEEEARQTFKLHIAGRRVVDLAGPEGT
                     TLAAVGTGHLTGIDPPLDGVRAHARDSRPVVELRVPFTVGREAVDDVERGSRDSDWQPV
                     KDAARTLARAEDRAVFEGYRAAEIEGIRAGSSNPPVQLPDDVREYPEAVSRALTALRLA
                     GVGGPYSLLLGAEAFTAVNETSDHGYPVHNHLARLLDGDIIWAPAIEGAFLLSTRGGDF
                     ELFLGQDVSVGYLSHDADSVQLYLQESLTFRVYTGEAAVALRG"
     gene            6062..6845
                     /locus_tag="O1G21_RS30365"
                     /old_locus_tag="O1G21_30365"
                     /pseudo=""
     CDS             6062..6845
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262570.1"
                     /locus_tag="O1G21_RS30365"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30365"
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSLRIVVCVKHVPDATGDRRFADDHTTDREGVDGLLSELDEYGVE
                     QALRIAEANGDAEVTVLTVGPDDAKDALRKALSMGADKAVHVNDDDIHGSDVIGTSAIL
                     AKALEKTGFDLVIGGMASTDGTMGVLGAAGGAAGCAAGDAAVRGVGRGRRGQGASGR"
     misc_feature    6620..6622
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6882..6902
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.92"
                     /tool="antismash"
     gene            6892..7854
                     /locus_tag="O1G21_RS30370"
                     /old_locus_tag="O1G21_30370"
     CDS             6892..7854
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020137074.1"
                     /locus_tag="O1G21_RS30370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30370"
                     /product="electron transfer flavoprotein subunit alpha/FixB
                     family protein"
                     /protein_id="WP_270148167.1"
                     /transl_table=11
                     /translation="MAEILVLVDHAEGAVRKPALELLTLARRIGEPSAVVLGAGAAAAD
                     IAAKAAEFGAAKVYVADGAEFADQLVVPKVDALAQIAKANDVAAVLVTSSSEGKEVAAR
                     VALRLGSGIITDAVDVRAGDQGPVATQSVFAASYTVDSRVSNGVPVITVKPNAVAPEAA
                     PAAGAVENVSVEFTGNAAKVTSRTPRVSTGRPELTEAAIVVSGGRGVGAAEGFGVVEEL
                     ADVLDAAVGASRAAVDAGWYPHSSQVGQTGKQVSPQLYVANGISGAIQHRAGMQTSKTI
                     VAVNKDPEAPIFELADYGVVGDLFEVLPQLTAEVKARKG"
     gene            complement(7983..8417)
                     /locus_tag="O1G21_RS30375"
                     /old_locus_tag="O1G21_30375"
     CDS             complement(7983..8417)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O1G21_RS30375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O1G21_30375"
                     /product="hypothetical protein"
                     /protein_id="WP_270148168.1"
                     /transl_table=11
                     /translation="MVHSAIDADTRSTTLYFQRCTWCNNPTFQRLLCPTCGSTELRLEP
                     SEGTGVVRRCAVEQARTPLVRQKSLIRMTEGFDVWCIVDGMRSTVPVGTRVRLYSSPGS
                     DQPELRLLPVEEPPVEQEAEPRPLPPLKGGFTQHLAAFAE"
     misc_feature    8410..8424
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.52"
                     /tool="antismash"
     misc_feature    8585..8599
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.4"
                     /tool="antismash"
     gene            8601..9752
                     /locus_tag="O1G21_RS30380"
                     /old_locus_tag="O1G21_30380"
     CDS             8601..9752
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560929.1"
                     /locus_tag="O1G21_RS30380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30380"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_270148170.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.1e-30, bitscore:
                     105.4, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 7.5e-28, bitscore:
                     95.4, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 3e-49, bitscore:
                     165.9, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQDFDLFRVSEEHEMLREAVRSLAEAKIAPFAAEVDEQGRFPQE
                     ALDALRGNDLHAVHVPEEYGGAGADALATVIVIEEVARVCGSSSLIPAVNKLGSLPVQL
                     SGSEELKAKYLGALARGEGMFSYCLSEPEAGSDAAGMKTRAVRDGDFWVLNGVKRWITN
                     AGVSEFYTVMAVTDPSMRSKGISAFVVEKGDEGVSFGAPEKKLGIKGSPTREVYFDNVR
                     IPADRMIGAEGTGFQTAMRTLDHTRVTIAAQAVGIAQGALDYAKGYVRERKQFGKAIAE
                     FQGIQFMLADMAMKLEAARQMTYVAAAKSQRGDADLTFFGAAAKCFASDVAMEVTTDAV
                     QLLGGYGYTRDYPVERMMRDAKITQIYEGTNQVQRIVMARNLP"
     misc_feature    complement(9813..9833)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.81"
                     /tool="antismash"
     gene            9897..10583
                     /locus_tag="O1G21_RS30385"
                     /old_locus_tag="O1G21_30385"
     CDS             9897..10583
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560930.1"
                     /locus_tag="O1G21_RS30385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1G21_30385"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_270151334.1"
                     /transl_table=11
                     /translation="MREALVAAAFELFLERGFEQTTIDDIVSLAGVGRRSFFRYFPAKE
                     DVVFPDHERGLAEMTEFLDSGTGEEDPVKHASDAARLVLRMYAEQPSFSVQRYRLTRKV
                     PGLRAYELSVVWRYEHTLAAYLRGRFADRPDGPLRADVIAAAVVAAHNHALRTWLRSGG
                     ESDWVAEVDAAMAFVTSTWSDRPTDTTPEGADATDDVVVLVASRRAPLWRMVQGIETAI
                     GDTRRI"
ORIGIN
        1 gggcacccag agctgctcgc cggtgatgtg gttgaccagc tcgcggacgg accagtcggc
       61 gcacggggtg ggggcgtccc actgcccggg cgtgaccagt cggacccgct cgtcgaaggc
      121 ggcgagcgcc tcggggtacg gcgtgccggg ccgggcggcc cgctggtgtt gcctgcggtg
      181 agccatgtct ccaccctgcc ccggtcccgg cggggcgcgc gagtcgctca tggaccgtca
      241 gttgttcaaa ttcgaactcc aaggtacggt cgaggagcgg ccggttcaaa tttgaacaac
      301 aggcgcgggc gcaccacacc acaccctgcc cggccgcacc gccacacaca cccctacgac
      361 cgaccccgat cgatggagac gcgcatgccg agcagcagcc cgagcacgac cgccgccacc
      421 agccccaccg cccgccgcgc ccggttcggc tccgggtccg cggccacctc cccgcacgcc
      481 tatgacgccg ggctcctgct cctgcgcctg gtgctcggcc tgaccatggc cgcgcacggc
      541 agccagaagc tgttcggctg gttcgggggc ggcgggatcg acggcaccgg tcagttcttc
      601 accatgagcg gctacccggc gggcaacgcc atggcggtgg tcgccggcct caccgagatc
      661 ctgggtggac tcggcctcgc cgtcggcctg ttgaccccgc tggcgggtgc cgccgtggtc
      721 ggcacgatgg tcaacgcgat cgccgtcacc tggggcggcg gcttcttcgc cccgaagggc
      781 aacgagtacg agctgctgct gacggcgggc gccgccgccc tcgcgctgac cggccccggc
      841 cgctacgcgg tggaccgctt cctgccggtg ctgaaggcgc accgcctggc gtacggcgtg
      901 gccgccgtgc tggtcggcct ggcgacggcc ggggtgatcc tgctgctgcg caagtagtcc
      961 ggcgccgtaa cgacaggcgc aaccagcctg accggaagcc gtggtgacgg atcgtcgcca
     1021 cggcttccgc gcgttccgcg cacgggccgc accgcgtcat tcgccgatcc gcaccagcgc
     1081 cagggtgatg ttgtccggcc cgcccgcctc gatcgccgcc ttccacagct cgaaggcggc
     1141 cttcccgtcg gcccgttcgg acgtgtcgta cgaccggccg agcagttcgg ccagttcctc
     1201 ctcggtcacc gggtcggtca ggccgtcgct gcacaccagg tagcggtcgc cggcggccag
     1261 cggcgagacc cgcacgtgcg gttcgacgac ggtgaggccg aacgagccgc ccagggtctg
     1321 ggtgaccacc gaggtggtgc gccggccggg cggcagcggc gggctgtcgt ccacgctcac
     1381 ccggtccagc ccgtctccgc tgacccggta gacccggctg tcgccgacgt tgaagaccac
     1441 ggcctgcccg ggcagcagca ccaccccggc gacggtggtg cccatcgcgg ccagctccgg
     1501 ctctccggcg gcggccgcgt ggaccgcccg gttgcactcc tgcagttcct cccggacggc
     1561 ctcctccccg tccagtccgc ccccggtggc ggcgagccgg cgggcgacca gcgcgctggc
     1621 cacctcgccg ccggggtggc cgcccagccc gtcggcgacc gcgaccacgc agggcgggcc
     1681 gagcgggaag agcagggtct gcgggttggt ggtcacggtg gcgcagagcg tccacgggcc
     1741 gactgcgaga ctgtcctcgt tgtgttcccg caccaggccc tcgtggctca gcgcgctcac
     1801 cgcgatgtac ggcgccatcg gttcgccgtc cttccggagg gtcccgagtc gtggtctccc
     1861 ggagggtccc gagtcgtggt cggggcccgc cttccattgt cggcgggaac cgggggaata
     1921 acgattcgcg cccggatgct aaggtggttg aaagttggac tacctgggac ctcctggagt
     1981 gtggtgagca gcatgcagtt cggaatcttc agcgtcggcg acgtcacccc cgacccgacc
     2041 accggccgca ccccgaccga gcacgagcgg atcaaggcca tggtggccat cgcgcgcaag
     2101 gccgaggagg tcgggctgga cgtcttcgcg accggcgagc accacaaccc gccgttcgtc
     2161 ccgtcctcac cgacgaccat gctcggctac atagccgcgc agaccgagcg cctcatcctc
     2221 tccaccgcga ccacgctgat caccaccaac gacccggtga agatcgccga ggacttcgcg
     2281 atgctccagc acctcgccga cggccgggtg gacctcatga tgggccgggg caacaccggc
     2341 ccggtctacc cctggttcgg caaggacatc cgccagggca tcccgctcgc gatcgagaac
     2401 tacgcgctgc tgcaccagct gtggcgcgag gacgtggtcg actggcaggg ccgcttccgc
     2461 accccgctgc agtccttcac ctccactccg cgcccgctgg acgacgtccc gccgttcgtc
     2521 tggcacggct cgatccgcag cccggagatc gcggagcagg ccgcctacta cggagacggc
     2581 ttcttcgcga acaacatctt ctggcccaag gagcacttcc agaagctgat cgacctctac
     2641 cgcgagcgct acgcccacta cggccacggg acgcccgagc aggcgatcgt cggcctcggc
     2701 gggcaggtgt tcatgcggaa gaactcccag gacgcggtgc gcgagttccg tccgtacttc
     2761 gacaacgcgc cggtgtacgg gcacggcccc tcgctggagg agttcaccga gcagaccccg
     2821 ctgaccgtgg gcagcccgca ggaggtcatc gagaagacgc tgagcttccg ggagagcttc
     2881 ggccactacc agcgccagct gttcctgatg gaccacgccg gcctgccgct gaagaccgtg
     2941 ctggagcaac tggacctact cggcgaggag gtcgtgccgg tgctgcgcga ggagttcgcc
     3001 aagggccgcc cggccgaggt gccggacgcg ccgacccacg ccgcgctggt cgcggcccgc
     3061 gacgcccggg agaaggagga ggtctccaag tgaccgcgtt caagctggcg gtggtgaccg
     3121 ccggactgag caagccgtcc tcgacccggc tgctggccga ccggctcgcc gaggcgaccg
     3181 tgcgccggct cgcccgggag gggcgggagg cggaggtcac cgtgatcgag ctgcgcgacc
     3241 tcgcggtgga catcgccaac gacttcgtca ccggcttccc ggcccccgcg ctgcgcgggg
     3301 cgatcgagac ggtgacggag gcggacgcgg tgatcgcggt gacgccgatc ttcaccgcct
     3361 cctacagcgg cctgttcaag tccttcttcg acgtgatcga ccacgacgcg ctcaccggca
     3421 agccggtgct gatcgccgcc accggcggca cgccccggca ctcgctcgcc ctggagcacg
     3481 ccgtgcggcc gctgttcggc tacctgcggg cggtcgtgct gccgaccgcc gtgtacgcgg
     3541 ccaccgacga ctggggcacg gcgggggacg cgctgaccga cggcctgccg ggccggatcg
     3601 accgcgcggc gggcgagctg gcggccctgc tggccgggcg cggccagacg gcgagggccg
     3661 cgctgcccga gcaggtggtc ccgttcgccg agcagctggc ggcgctgcgg gtctgacgcc
     3721 ggacggccga ccgcggacgg ccgagggggc ggatgcgcga aggagcgcac ccgccccctc
     3781 ggcatgcgct cgggcagcgc ggcccggcca cccgaacggc ccctgcggag cggtctccgc
     3841 cggtgccgcc ggcgccttcg gtgggaccgt tcggctgcgt gtcggccgtg ccgatgcgtc
     3901 cgttgtgcgg tgtcatcgtt ggcatgcagc ccgaaccgca agccgtgctc tccccgctga
     3961 ccggcgcggc ggtcttcctg gtcctcaccg tcgaacccgg cggcgaggag gccgtccgcg
     4021 acctcctgcc cgatctcgcc ggactgcgcc ggtccgtcgg cttccgggcc cccgacggcg
     4081 ccctcacctg cgtcaccggc atcggctccg cactctggga ccgcctcttc gacggccccc
     4141 ggcccgccga cctccacccc ttccaggaac tggccggccc ccggcaccgc gccccggcca
     4201 cccccgggga cctgctgctc cacctgcggg ccgagcggat ggacctctgc ttcgaactgg
     4261 ccggccagct caccacccgc ctcgccgggg cggccaccgt ggtcgactcc gcgcacggct
     4321 tccggtactt cgacgaccgg gacctgctcg gcttcgtcga cggcaccgag aaccccgagg
     4381 gccgggccgc cgtggacgcc gccctggtcg gggcggagga cccggacttc gccggcggca
     4441 gctacgtcat ggtgcagaag tacctgcacg acctggacaa gtggaacgcg ctgagcgtcg
     4501 agcagcagga gggggcggtc ggccgcacca agctcaccga catcgaactg gacgacgacg
     4561 tcaaacccgc cgactcgcac gtggccctca ccgtggtcac cggcccggac ggcgaggaac
     4621 ggaagatcgt ccgctacaac atggccttcg gctccttcga gcgcggcggc gagttcggca
     4681 cctacttcat cggctacgcg cgcgaccccg gggtgaccga ggagatgctg cgcaacatgt
     4741 tcctcggcga cccgcccggc accaccgacc ggctgctcga cttctccacc gccgtcaccg
     4801 gctcgctgtt ctacgtcccg agcgccgact tcctcgacgc cccgccgccc gcccccgccg
     4861 ctcccgctgc cccagccggc cccgccgccg aggcccgtgc ggacggctcg ctcggcatcg
     4921 gcagcctcaa gccgacctcc tgaagccgac gtcccgaagc cgaccgcctg gaagcagccc
     4981 tcccgaacag gacccgcacc gtgaacaacc tgcaccgcga actcgccccc gtctccgccg
     5041 ccgcctggga ggagatcgag gaggaggccc ggcagacctt caagctgcac atcgccggcc
     5101 gccgggtggt cgacctcgcc ggccccgagg gcaccaccct cgccgccgtc ggcaccggcc
     5161 acctcaccgg catcgaccca cccctcgacg gcgtccgggc ccacgcccgc gacagccgcc
     5221 cggtggtcga actgcgcgtc cccttcaccg tcggccggga ggccgtcgac gacgtcgagc
     5281 gcggctcccg cgactccgac tggcaaccgg tcaaggacgc cgcccgcacc ctggcccgcg
     5341 ccgaggaccg cgccgtcttc gagggctacc gggccgccga gatcgagggc atccgggccg
     5401 gctccagcaa cccgccggtc cagctcccgg acgacgtacg ggagtacccc gaagccgtca
     5461 gccgcgccct caccgccctc cgcctcgccg gggtcggcgg accgtactcg ctcctgctcg
     5521 gcgccgaggc cttcaccgcc gtcaacgaga ccagcgacca cggctacccc gtgcacaacc
     5581 acctggcgcg gctgctggac ggcgacatca tctgggcccc cgcgatcgag ggggcgttcc
     5641 tgctgtccac ccggggcggg gacttcgaac tcttcctcgg ccaggacgtc tcggtgggct
     5701 acctctccca cgacgcggac tcggtgcagc tgtaccttca ggagtcgctc accttccgcg
     5761 tgtacacggg agaggcggcc gtcgcgctcc ggggctgacg cccggacccc gtgtccgcct
     5821 ccggccgccg cgcccgatcg ccgggtaggg tactgagtgc ctttacgagg ggtactgagt
     5881 gccgtattct ctcggtggag cctctgacgc cagtcgaaga aagccgacac ggcatgccgg
     5941 tccaggtggc gcttgcggcg cgcgggccgc ggccgggccg gacttggcac tcagtaccct
     6001 cggcctgacc tgaggccatg gatccagtgc atccgtgcac ccgtgacagg agaaaccgct
     6061 catgagcttg aggatcgtcg tctgtgtgaa gcacgtgccg gacgcgacgg gtgaccgtcg
     6121 tttcgcggac gaccacacca ccgaccggga gggtgtggac ggcctcctgt cggagctgga
     6181 cgagtacggc gtcgagcagg cgctgcggat cgccgaggcg aacggcgacg ccgaggtgac
     6241 ggtgctcacg gtgggtccgg acgacgcgaa ggacgcgctg cgcaaggcgc tgtcgatggg
     6301 tgcggacaag gccgttcacg tgaacgacga cgacatccac ggctcggacg tgatcgggac
     6361 gtcggcgatc ctggcgaagg cgctggagaa gaccgggttc gacctggtga tcggcggcat
     6421 ggcgtcgacg gacggcacga tgggtgtgct gggcgctgct ggcggagcgg ctgggtgtgc
     6481 cgcaggtgac gctgctgtcc gaggtgtcgg tcgagggcgg cgtggtcaag gggcgtcggg
     6541 acggtgacgc ggcgaccgag ctggtcgagg cggcgctgcc ggccgtggtc tcggtgacgg
     6601 accagtcggg tgaggcccgt tacccctcgt tcaaggggat catggcggcg aagaagaagc
     6661 cggtggagtc gctggacctg gacgacctgg gcatcgaggc ggacgaggtc ggcctggcgg
     6721 gttcgtggac ggcggtggag tcggtggcgg cgcgtccggc gcgtaccgcg ggcacggtcg
     6781 tcaaggacga gggcgagggc ggcaagcagc tcgccgcctt cctcgccgac cagaagttca
     6841 tctagtccgc accccgaccc ccgaaccagc gaaacacgga gacatagagc aatggctgag
     6901 attctcgtcc tggtggacca cgccgagggt gcggtccgca agcccgccct ggagctgctg
     6961 accctggccc gccgcatcgg cgagccgtcc gccgtcgtgc tgggcgccgg tgccgcggcc
     7021 gccgacatcg ccgccaaggc cgccgagttc ggcgccgcca aggtgtacgt cgcggacggc
     7081 gccgagttcg ccgaccagct ggtcgtcccc aaggtggacg ccctggccca gatcgccaag
     7141 gccaatgacg tggccgccgt cctggtgacg tcctccagtg agggcaagga ggtcgccgcc
     7201 cgcgtggcgc tgcgcctcgg ctccggcatc atcaccgacg cggtggacgt ccgcgccggc
     7261 gaccagggcc cggtcgccac ccagtcggtg ttcgccgcct cgtacaccgt cgactcccgc
     7321 gtcagcaacg gcgtgccggt catcacggtc aagccgaacg ccgtcgcccc ggaggccgcc
     7381 ccggccgccg gcgcggtcga gaacgtgtcg gtggagttca ccggcaacgc cgccaaggtc
     7441 acctcgcgca ccccgcgcgt ctcgaccggc cgcccggagc tgaccgaggc cgccatcgtg
     7501 gtctccggcg gccgtggcgt cggtgccgcc gagggcttcg gcgtggtcga ggagctcgcg
     7561 gacgtgctcg acgccgccgt cggtgcctcg cgcgccgccg tcgacgccgg gtggtacccg
     7621 cacagctccc aggtcggcca gaccggcaag caggtctccc cgcagctgta cgtggccaac
     7681 ggcatctcgg gcgccatcca gcaccgggcc ggcatgcaga cctcgaagac catcgtggcc
     7741 gtcaacaagg acccggaggc cccgatcttc gagctcgccg actacggcgt ggtcggggac
     7801 ctcttcgagg tgctgccgca gctcaccgcc gaggtgaagg cccgcaaggg ctgatccgcg
     7861 cagcggaaga acggagggcg tggcactcgg taccccggta ccgagtgcca cgccctccgt
     7921 cgcgtcgcgc ctcgtcgcat ggccgacagg ggcccgcggt ccgccgcggg gcccgtcgcg
     7981 gcctactcgg cgaaggccgc caggtgctgg gtgaacccgc ccttgagcgg cggcagcggg
     8041 cgcggctcgg cctcctgctc caccggcggc tcctccaccg gcagcagccg caactcgggc
     8101 tggtcggagc cgggggagga gtacagccgc acccgggtgc cgaccgggac ggtgctgcgc
     8161 atcccgtcga cgatgcacca gacgtcgaac ccctccgtca tccggatcag cgacttctgg
     8221 cggaccagcg gcgtgcgggc ctgctcgacc gcgcagcgcc gcacgacgcc ggtgccctcg
     8281 ctgggctcca gccgcagctc ggtggagccg caggtggggc agagcagccg ctggaaggtg
     8341 gggttgttgc accaggtaca gcgctggaag tacagcgtcg tgctccgggt gtcggcatcg
     8401 atggctgagt ggaccacgtt gtcatctccc tgcgctcgga ccggctctgt cggcagaagt
     8461 acgtcagtag ttcggctgta gtacgacagg aaaaggatag ggaactcagt gccgccctgt
     8521 gaaggcactg agttcccttt ttgccgcgcg catgcataat gaagggtact gagtaccgtc
     8581 gtgaaacagg agtggcgatc atgagccagg acttcgatct cttccgcgtc tccgaggagc
     8641 acgagatgct ccgtgaggcg gtgcgttcgc tggccgaggc gaagatcgct ccgttcgcgg
     8701 cggaggtgga cgagcagggg cggttcccgc aggaggcgct ggacgcgctg cggggcaacg
     8761 acctgcacgc ggtgcacgtg ccggaggagt acggcggtgc gggggcggac gcgctggcga
     8821 cggtgatcgt gatcgaggag gtcgcgcggg tgtgcggctc gtcgtcgctg atcccggcgg
     8881 tgaacaagct ggggtcgctg ccggtgcagc tgtcgggttc ggaggagctg aaggcgaagt
     8941 acctgggggc gctggcgcgg ggcgagggga tgttctcgta ctgcctgtcg gagccggagg
     9001 cgggttcgga cgcggcgggg atgaagaccc gggcggtgcg cgacggtgac ttctgggtgc
     9061 tgaacggtgt gaagcggtgg atcaccaacg cgggggtctc cgagttctac acggtgatgg
     9121 ccgtcaccga tccctcgatg cggtcgaagg gcatctcggc gttcgtggtg gagaagggcg
     9181 acgagggcgt gtcgttcggt gcgccggaga agaagctggg gatcaagggt tcgccgacgc
     9241 gtgaggtgta cttcgacaac gtgcggatcc cggcggaccg gatgatcggt gcggagggca
     9301 ccggtttcca gacggcgatg aggacgctgg accacacccg ggtgacgatc gcggcgcagg
     9361 cggtgggcat cgcgcagggt gcgctggact acgcgaaggg ctacgtccgg gagcgcaagc
     9421 agttcgggaa ggcgatcgcg gagttccagg ggatccagtt catgctggcg gacatggcga
     9481 tgaagctgga ggccgcgcgg cagatgacgt acgtggcggc ggcgaagtcg cagcgcgggg
     9541 acgcggatct gacgttcttc ggtgcggcgg cgaagtgctt cgcgtcggac gtggcgatgg
     9601 aggtgaccac ggacgcggtg cagttgctgg gtggttacgg ctacacgcgg gactacccgg
     9661 tggagcggat gatgcgggac gcgaagatca cccagatcta cgagggcacc aaccaggtcc
     9721 agcggatcgt gatggcccgc aacctgccgt aacggcgctc ggacgtgccc cgacggccgg
     9781 acgggccagc ccaccggccg tcggggccga cgcatcgcgc cgatgctaag ttccggagaa
     9841 tgagcgacaa ggaagagcag gacctgccca tgaacgacgg gccctcgaag ccggcgatgc
     9901 gcgaggcgct ggtggccgcg gccttcgagc tcttcctgga acgcggcttc gagcagacga
     9961 cgatcgacga catcgtctcg ctcgccggcg tcggccgacg ctccttcttc cgctacttcc
    10021 cggccaagga ggacgtggtc ttccccgacc acgagcgcgg cctcgccgag atgacggagt
    10081 tcctggactc cggcaccggc gaggaggacc cggtcaagca cgccagcgac gcggcccgcc
    10141 tggtcctgcg gatgtacgcc gagcagccct ccttctccgt ccagcgctac cgcctcaccc
    10201 gcaaggtccc cggcctgcgg gcgtacgagc tgtcggtggt ctggcgctac gagcacaccc
    10261 tcgccgccta cctgcgcggc cgcttcgccg accgcccgga cggcccgctg cgcgccgacg
    10321 tgatcgccgc cgccgtggtc gccgcccaca accacgccct gcgcacctgg ctgcgctcgg
    10381 gcggcgagag cgactgggtg gccgaggtcg acgcggcgat ggccttcgtc acctccacct
    10441 ggagcgaccg ccccaccgac accacccccg agggcgccga cgcgaccgac gacgtggtcg
    10501 tcctggtcgc cagccgccgg gccccgctgt ggcggatggt ccagggcatc gagaccgcca
    10561 tcggcgacac ccgccggatc tgacggccgg tgggcccggc gccccggcgg cgctcgccgt
    10621 cggcggcgaa caccgaacac cccgagccga cagccgctct gacgggcaga tgccttggcg
    10681 atgcggtggt gaccgagatg ctccgcccta cccggggaaa gcgaaaggcc ccagatgaac
    10741 tggggcctta gccggtgtcc gaggggggac ttgaaccccc acgcccgata aagggcac
//
