LOCUS       NZ_CP114205            10921 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces tubercidicus strain DSM 40261 chromosome, complete
            genome.
ACCESSION   NZ_CP114205
VERSION     NZ_CP114205.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tubercidicus
  ORGANISM  Streptomyces tubercidicus
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8284047)
  AUTHORS   Ruckert,C., Busche,T., Kalinowski,J. and Wittmann,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-MAR-2022
            Assembly Method                   :: flye v. 2.9; Newbler v. 2.8
            Assembly Name                     :: DSM40261_v1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133.7x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:53:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,167
            CDSs (total)                      :: 7,077
            Genes (coding)                    :: 6,906
            CDSs (with protein)               :: 6,906
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 72 of 171
            Pseudo Genes (incomplete)         :: 133 of 171
            Pseudo Genes (internal stop)      :: 9 of 171
            Pseudo Genes (multiple problems)  :: 41 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:04:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8205504
            Orig. end                         :: 8216425
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114205.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from DSM 40261.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10921
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="35"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10921
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10921
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5921](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5921)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            297..3746
                     /db_xref="GeneID:96287984"
                     /locus_tag="STRTU_RS35560"
                     /old_locus_tag="STRTU_007115"
     CDS             297..3746
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96287984"
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /locus_tag="STRTU_RS35560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_007115"
                     /product="response regulator"
                     /protein_id="WP_159749545.1"
                     /transl_table=11
                     /translation="MKRSVGRRVSVSLRMLVRVFVLVNALLLAVVLTLSVGAVQSMQDA
                     ARAESRRSESLRLADELRQTSDDLTRMARTYAVTGESRYRDYFVEILAIRDGRAPRPEQ
                     YDRVYWDVVTDTGRRPTSSGPAASFDTLAARAGFTGQELSLLKSARSRSDALARIEEAA
                     FAEIDSTAGTSRAAAGRDRAAALLYGADYHHAKAQIMQPIGRVFELVDVRTHRETAQAV
                     GRATGYSSAAVGVALVMLCGMGVVAVVARRAIIRPVTMLDHATARIAEGDLRVRAPVGG
                     VSEISSLATRFNGMTERVRLRTGELELLQRVAVTANEAADLTEATQVVLDLVCGYTGWQ
                     VGHAYWLAPGAARAEGAKLVPSQIWHSDDSAEFRSFRQAREASPLTPGKGLPGRVFASG
                     KAVWIVDVTKTPEFSGVGHADDIGVHAAMGFPVLVGDEVVAVLEFFSFQLAEPDGVLLD
                     LMGNIGTQLGRVVDRMRAETAARNVNLLLESTAEGIYATDLQGDCTFVNKAAAEILGYE
                     PDSLVGNHMHELCSHAQRNGLLWSIEECPICAAYRTGRGRQTEGEMFRRRNGTVFPVAC
                     TSYPISEKGEVRGAVVAFSDVTAQRRAEEALQQAKEAAESANNAKSTFLATMSHEIRTP
                     MNTIIGMTGLLFDTELDHEQRDFAGIVRESAGALLTLINDILDFSKIEAGKLKLEHQPF
                     HVDECVETALELVAAQAAAKNIELACLVDPDAPEGMVGDVTRLRQVLLNLLSNAVKFTE
                     RGEIVVTVDAEPLADAGAGPTASPRYRWHFAVRDTGIGIAPNRIDGLFDLFSQVDVSTT
                     RRYGGTGLGLAISKRLCELMGGTIFVSSQQGEGSTFRFTVEGETAAVRRRGRVPEARPE
                     LAGKRVLVIDGSAVNRQILMTQMASWGMQPVTTESPAEALEWLSAGAQFDLGILDAKMP
                     EGNGPTLTETVQATQQHRSIPLIMLTSLGQQRNDLPNSQIAAYLTKPIRPSQLFDAIIS
                     VFTGQLAGPVSAVERTPEKHRSVPPGTLRILVAEDHAVNQRLAVLLLDKLGYRADVAST
                     GLEVLAALERQTYDVILMDVQMPEMDGLEATQRIRSRWPGEGRPYIVAVTANAMEGDRG
                     PCIEAGMDDYLTKPINLKELGNALARCARRGEPTHGDFGSSG"
     gene            complement(4045..4764)
                     /db_xref="GeneID:96287985"
                     /locus_tag="STRTU_RS35565"
                     /old_locus_tag="STRTU_007116"
     CDS             complement(4045..4764)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96287985"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151397.1"
                     /locus_tag="STRTU_RS35565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_007116"
                     /product="HAD family phosphatase"
                     /protein_id="WP_159749547.1"
                     /sec_met_domain="HAD_2 (E-value: 6.6e-27, bitscore: 93.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTADSILSWTPAAVVFDCDGTLMDTERHWEDARDMVLHSYGCAPS
                     HEFAEKARGLHYTECGRLMADVAGRPELTGDMTESLLKHFRELVAADPTTMPGAPELVH
                     STVQFAPLAVASNCPLEVVESCLGMAGLRDCFSHIVVPDDTTRPKPHPDVYLTAAQLCG
                     VDPADTLAVEDSHCGIQAAAGAGMRVLGVGPRPSDRETALADWWVHSLAEPAVQGWADA
                     RIPAQGTGPTGPGTTTA"
     misc_feature    complement(4576..4578)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4926..4941)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    4975..4990
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 19.79"
                     /tool="antismash"
     gene            complement(5001..5921)
                     /db_xref="GeneID:96287986"
                     /locus_tag="STRTU_RS35570"
                     /old_locus_tag="STRTU_007117"
     CDS             complement(5001..5921)
                     /codon_start=1
                     /db_xref="GeneID:96287986"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543192.1"
                     /locus_tag="STRTU_RS35570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_007117"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_159749549.1"
                     /sec_met_domain="AfsA (E-value: 3.5e-15, bitscore: 54.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSTVRVVGKSEPLSWSRTVERELVHRTSVAEVLLTDVRPADTP
                     HAFLAAASWPRAHATFPRDGSQRHSPLILVETLRQLGLYIPLRFYAVPEVFRAVITDLF
                     FHLSPADELPARAGATEITCSARVSAVRRAPDESAVGLRLNVTFAACGVTFGQGGGGVR
                     FLDGARFTALRARSAVDAVPARPPEGGSRPPAAQLTVNHPHDALLALDQGELLVSPADP
                     LHPFFFDHPTDHVPGMVLLEAARQAASYRSRGRLSRPLWGRLNASRFTELTPPARVLCT
                     RHGSTCAFRILQGGTETACGTFNYT"
     gene            6027..6641
                     /db_xref="GeneID:96287987"
                     /locus_tag="STRTU_RS35575"
                     /old_locus_tag="STRTU_007118"
     CDS             6027..6641
                     /codon_start=1
                     /db_xref="GeneID:96287987"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566181.1"
                     /locus_tag="STRTU_RS35575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_007118"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_246241717.1"
                     /transl_table=11
                     /translation="MQIRAEATRRFLLEAAATLFDEKGYAGTSISDISAYSGRTSGAIY
                     FHYASKEKLALAIVEEHFATWPHLISRFRQPGLPALEKLVGLSFEVARAFRDDIVVRAG
                     ARLWAERKAIDAQLPAPFLGWIEAVTQLLEEAARNGELAADVPPAATAHGIVCSFFGLH
                     TVSDALEGRRHIEERLHDLWTLLLRGLQAEPDPAGLLDRVR"
     gene            complement(6891..7439)
                     /db_xref="GeneID:96287988"
                     /locus_tag="STRTU_RS35580"
                     /old_locus_tag="STRTU_007119"
     CDS             complement(6891..7439)
                     /codon_start=1
                     /db_xref="GeneID:96287988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676823.1"
                     /locus_tag="STRTU_RS35580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_007119"
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="WP_246241654.1"
                     /transl_table=11
                     /translation="MAPTARTAVAWLPPRKLWPVIQDIRWEHDPQVRRWPPHVNLLFGF
                     VPEEAFARAVPLLSAGAAQSTAFTARLAGVRFFRHRRYATLWLDPAAADPAPWAHLHRE
                     LGLRFPLCHGRAGRFTPHLSLGRTQDPKRLAAECAVRLGALSAVVEEVVLLSRRGEEPM
                     RPRAVITLGTGEVRRPQEC"
     gene            complement(7795..9519)
                     /db_xref="GeneID:96287989"
                     /locus_tag="STRTU_RS35585"
                     /old_locus_tag="STRTU_007120"
     CDS             complement(7795..9519)
                     /codon_start=1
                     /db_xref="GeneID:96287989"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1160: flavin reductase domain protein FMN-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /locus_tag="STRTU_RS35585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_007120"
                     /product="styrene monooxygenase/indole monooxygenase family
                     protein"
                     /protein_id="WP_159749555.1"
                     /transl_table=11
                     /translation="MRRIAVVGAGQAGAQLALGLQAHGYDVTLVTDRDPDEIRRGPVMS
                     SQCMFDTALQTERELGLHHWEDHAPDISGIAFSLIGPHGTPDVSWRAPLEGPAHSVDQR
                     VKCAAWIEQFADGGRGEIVLHEAGVNDLEWYARTHDLVVVSTGKGELSQLFPRNPELSP
                     YDRPRRALALTYVTGTAPREGEDAVHYRLVPGVGEYFSFPALTTTGPCDIMVFEGVPGG
                     PMDCWDDIRTPEGHLTRSLEVLHRFFPDEYERCRHARLTDNNGVLRGRLTPTVRRPVAR
                     LASGRHVLGMADAVVLNDPITGQGSNNAAQAATHYLDSILRHGTAEFTPQWMQRTFDNF
                     WRGWAQWAVGWTNSLLADLSPHHRDLLTAAAEIPSVAGALAAGFDDPRTLYRWWFEEAE
                     AHRFLAEKRAQHAARFDGRELRRALGQYATGVTVVTARSPDGRNVGMTANSFTSVSMNP
                     PLVLWCPGKNSPSLPDFTDASHFAVHILAADQHHLSRQFATPADDKFRGTPTTPGIAGT
                     PLLDGAVARFQCRTVQRLDAGDHIIFLGEVEQYEAEGGTPLVFHSGYYQVATKHPDL"
     gene            complement(9591..10811)
                     /db_xref="GeneID:96287990"
                     /locus_tag="STRTU_RS35590"
                     /old_locus_tag="STRTU_007121"
     CDS             complement(9591..10811)
                     /codon_start=1
                     /db_xref="GeneID:96287990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606881.1"
                     /locus_tag="STRTU_RS35590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_007121"
                     /product="styrene monooxygenase/indole monooxygenase family
                     protein"
                     /protein_id="WP_159749556.1"
                     /transl_table=11
                     /translation="MRKITIVGAGQAGLQLGIGLIDHGFDVTLVSNRTGDQIREGRVMS
                     SQAMFGTALAHERNLGLDFWGDACPSIDGLGVSIADGQGGRALSFDARLEATARSIDQR
                     VKMPLWMGEFARRGGRLELCEAGIEDLERYAAESDLVIVAAGKGEIAGLFERDAMRSPY
                     DAPQRALALAYVTGMAALPDRPGVSFNVIPGVGEYFTMPSLTTSGPCDIMFFEGIPGGP
                     LDCFDGLTPHQQLAKFQELLRTYVPWEAERCTGVTLTDENGTLAGRFAPTVRKPVATLP
                     SGAQVLGLADVVVLNDPITGQGSNNASKCAASYLATILDHCERPYDAAFMELAFGRFWD
                     NAGAATTWTNAMLGAPPQHVLELFGAASANSRIAARFVNGFDDPRDLFHWFMDPVAAKN
                     YLAEVGA"
     misc_feature    10841..10850
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.44"
                     /tool="antismash"
     misc_feature    10877..10893
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 22.52"
                     /tool="antismash"
ORIGIN
        1 aatgacctga gagccgtact ggacaactgc acccccctgg tatcaggtgc ccccgatgat
       61 cctcacgcag acggatagaa gaatggccag gcgccgtcca ccgatcaagg actcgacacc
      121 tcacccgcta ccgggaacaa gcacgccagg gaagcccgcc gacccgttgc acaggtcacc
      181 ggttggatac cctgggaatc ggcaaatggc accgggctca ttagggtgtg agagccggct
      241 acacaccgag atcgacgaga gacgccaggg cgtgtcctgt cggaggagtg gtggcgatga
      301 agcgttctgt ggggcggcgt gtgagtgtgt cgctccgaat gctcgtacgt gttttcgtcc
      361 tggtcaatgc cctattgctt gccgttgtgc ttaccctgag cgtgggtgct gtgcagagca
      421 tgcaggacgc tgcacgggcc gagagccggc ggtcggaatc gctgcgcctg gcagacgaac
      481 tgcgccagac ctccgatgac ctgacccgca tggcaaggac gtacgcggtc accggagagt
      541 cacgttaccg ggactacttc gtcgagatcc tggctattcg tgacggtaga gcaccgcgcc
      601 ccgagcagta cgaccgtgtc tactgggatg tggtcaccga caccggacgg cgacccactt
      661 cctctggacc ggctgccagc ttcgatacgc tggccgcgcg ggcagggttc accggtcaag
      721 agttgagcct cctcaagtcg gcgcggtcgc gctccgacgc tctggcgcga atcgaagagg
      781 cggccttcgc cgagatcgac agcaccgccg gaacttccag ggcagccgcc ggccgggaca
      841 gggctgccgc actgctctac ggcgctgatt accatcacgc caaggctcag atcatgcaac
      901 cgataggacg cgtgttcgag ctggtcgacg tgcgtacaca ccgagaaacc gcgcaggcag
      961 tcggccgggc gactggatac tcgtcggcag cggtcggcgt cgcactggtg atgctgtgcg
     1021 gtatgggggt cgtcgcggtt gtggcgcggc gagcgatcat ccgtccggtc accatgctcg
     1081 accacgctac tgcgcgcatc gccgagggcg acttgcgtgt gcgtgcgcca gtcgggggcg
     1141 tcagcgagat cagctcactc gccacgcgct tcaacggcat gactgagcgg gtacggctcc
     1201 gaaccggaga gcttgagctg cttcaacgcg tcgcggtcac cgccaacgag gccgctgacc
     1261 tgactgaggc gacgcaggtt gtcctcgacc tcgtctgcgg ctacaccggc tggcaggtgg
     1321 gccatgcata ttggctcgcc cctggtgcgg cacgggccga gggcgccaag ctggtcccct
     1381 cgcagatctg gcactccgat gactctgcag agtttcggtc atttcggcag gcaagggaag
     1441 ccagcccgct gacccctgga aaggggttgc ccggacgtgt atttgccagc ggtaaggctg
     1501 tgtggatcgt cgatgtgacc aagacccctg aattctccgg agtcggccat gcggacgaca
     1561 tcggtgtgca tgccgccatg ggattccccg tgctggtggg cgacgaagtc gtcgccgtgc
     1621 tggaattttt ctcattccag ctggccgagc ccgacggggt attgctcgac ctgatgggaa
     1681 acataggcac gcagcttggc cgagtcgtgg accgtatgcg tgcagagact gcggcacgca
     1741 acgtcaattt gttgctggaa tcgaccgccg aaggtatcta cgctaccgac ctccagggag
     1801 actgcacgtt cgtcaacaag gccgcagccg agatactggg atacgaaccc gactccctgg
     1861 tcggcaatca catgcacgag ctgtgcagcc acgcccagcg caacggtctt ctctggtcga
     1921 tcgaggaatg tccgatctgt gcggcgtatc ggaccggtcg tgggcgccag accgaggggg
     1981 agatgttcag aaggcgcaat ggaactgtct ttcctgtagc ttgcacctct tacccgatca
     2041 gtgagaaggg ggaggttcgt ggggcggtcg tcgccttctc ggacgtcaca gcccagcgac
     2101 gcgccgagga ggcgctgcag caggcgaagg aggcagccga gagcgccaac aacgccaaga
     2161 gcacttttct ggcgacgatg agccacgaga tccgtacccc gatgaacacg atcatcggca
     2221 tgaccggact actgttcgac acggaactcg accacgagca gcgcgacttt gccggcatcg
     2281 tccgtgagag cgcgggagcc ctgctcacgc tgatcaatga cattctcgac ttctcgaaga
     2341 tcgaggccgg taaactcaag cttgagcacc agccgttcca tgtcgacgag tgcgttgaga
     2401 ccgcgctcga actcgtggcc gcccaggcag ccgcgaagaa cattgagttg gcctgcctgg
     2461 ttgacccgga tgcgcccgag ggcatggtgg gtgacgtcac ccgcctccgg caggtgctgc
     2521 tcaatctgct gagtaacgcg gtgaaattca ccgaacgagg cgagatagtg gtcacggtcg
     2581 acgccgagcc tctggcagac gccggcgccg ggccgaccgc gtccccgagg taccgatggc
     2641 actttgctgt gcgggatacc ggaatcggca tcgcgcccaa tcgaatcgac ggactttttg
     2701 atctgttcag tcaggtcgat gtctcaacga cccgccgcta cgggggcacc ggtcttggcc
     2761 tggccatctc aaagcgcctg tgtgaactca tgggcgggac gatcttcgta tccagtcagc
     2821 aaggtgaggg ctcgacattc cgcttcacag tcgagggtga gacggcagcc gtccggcggc
     2881 gcggccgtgt accggaggca aggccggagc tggcgggaaa gcgggtgctc gttatcgatg
     2941 gaagcgcggt caaccgtcag atcctgatga cacagatggc gtcatggggc atgcagccgg
     3001 ttaccaccga gtcaccggcc gaagcgctcg aatggctttc ggccggagcc caattcgacc
     3061 taggcatcct tgacgcaaag atgccggagg ggaatggacc caccctcacc gaaaccgtac
     3121 aggccaccca gcagcatcgc tccatcccgc tcatcatgct gacttcgctc ggtcagcagc
     3181 gcaacgacct ccccaattcc cagatagccg cctacctgac caagccgatc aggccgtccc
     3241 agctctttga cgccatcatc agcgtcttca cagggcagtt ggccggtcct gtgtctgccg
     3301 tggagcgcac gcctgaaaag catcggagcg tgcccccggg aactttgcgg atcctcgtcg
     3361 cggaagacca cgcggtcaat cagcggctgg cagtactgct gctggacaaa ctcggatacc
     3421 gagccgacgt cgcgagcact ggtctggagg tattggctgc gctcgaacgt caaacctacg
     3481 acgtgatact catggacgtg cagatgccag agatggatgg cctggaggcg acccagcgca
     3541 tccgctcgcg ttggccgggc gagggtcgcc catacatcgt cgccgtcact gccaacgcaa
     3601 tggagggcga tcgggggccg tgcatcgaag ccgggatgga tgactacctc accaagccca
     3661 tcaatctcaa ggagctgggc aacgcactcg cccgctgtgc acgccggggc gagcccactc
     3721 atggtgactt cggctcgtca gggtgacgct ggggtgtcgt acaagcgcga tgacgaaccg
     3781 gccgtcgggc ccttagaggc accaccccac ccacttctgg atccgggtgg acgctgtggc
     3841 cgatcccaag ctgatagccg gttactacag ggaggtcgcg ccgggcgcca ccatgaaagt
     3901 gaaccagccg ggcgcgaagg ccccctcgat ggcgggtgtg cccacgcgcc acggtcttcg
     3961 gcgccctgga gcttcggggg gcgccgggtt ctcctacccc gggcccgcgg cccgtcatgg
     4021 agtctgccgc gggccgggcc gccgtcatgc cgtcgtggtc ccgggcccag tgggtccagt
     4081 gccctgggca gggatgcggg cgtcggccca tccctgcacc gccggctctg ccagcgagtg
     4141 gacccaccag tcggccaatg ccgtctccct gtcgctgggg cggggaccga ctccgagtac
     4201 ccgcatgccc gcgcctgcgg cagcttggat gccgcagtgg gagtcctcga cggccagcgt
     4261 gtcagcgggg tccacgccac agagttgggc ggcggtgagg tagacgtccg ggtgaggttt
     4321 gggtctcgtg gtgtcgtcgg gtaccacaat gtggctgaag caatcccgga gtccagccat
     4381 tcccaggcag gactcgacga cttcaagtgg gcagttactg gccacggcca gcggggcgaa
     4441 ctgcacggtc gaatgcacca attccggcgc cccgggcatg gtggtggggt ctgcggcgac
     4501 gagttcacgg aagtgcttga gcaatgattc cgtcatgtcc ccggtcagtt ccggtcgccc
     4561 ggccacatcg gccattaagc gcccgcattc ggtgtagtgc agccctcgcg ccttttccgc
     4621 gaattcgtgc gagggcgcgc agccgtagct gtgtagcacc atgtcacggg cgtcctccca
     4681 gtggcgttcg gtgtccatca gcgtgccgtc gcagtcgaaa acgacggccg cgggggtcca
     4741 ggaaaggatg ctgtctgcgg tcattcggga ttccgttctg atggggcccg aaggcgccga
     4801 tgcgaccggt cgcgaggaag gcagggcagc gtcggtttca cggacgcccg gcatcgggcc
     4861 gagggattcc ggcagggcag ctcccaaggc agctcccatg gcgaagagtt cttccgctcc
     4921 agccggaaaa taacgatcgt tacgttacgg atggaagcgc agaaaggtca accaattcac
     4981 aaatgtcaat attgtgtgtt ctacgtataa ttgaaggttc cgcaagccgt ctctgtgccg
     5041 ccctggagga tgcggaatgc acacgtcgag ccgtgccggg tgcacagcac gcgtgcgggt
     5101 ggggtcagtt cggtgaaccg ggaggcattc agccggcccc aaaggggccg gctcagcctg
     5161 ccccggctgc ggtacgaggc ggcctggcgg gcggcttcca acagcaccat gccaggcacg
     5221 tgatcggtgg ggtggtcgaa gaagaacggg tgcagcgggt cggccggcga gaccaacagc
     5281 tcgccctggt cgagagcgag gagcgcatca tgcgggtgat tcacggtcag ctgtgcggcc
     5341 ggcgggcgcg acccgccctc cggagggcga gccgggacgg catcgaccgc gctcctggcg
     5401 cgcagcgcgg tgaagcgcgc gccgtccagg aaccgcacgc ccccgccacc ttggccgaag
     5461 gtcaccccgc acgcggcgaa ggtgacattc agtcgcagtc cgacggcgga ctcgtccggc
     5521 gcccgccgta cggcgctgac ccgtgccgag caggtgattt cggtcgctcc ggcgcgggcc
     5581 ggcaactcgt cggcagggct gaggtggaag aacaggtcgg tgatgaccgc acggaagacc
     5641 tccggaaccg cgtagaaccg caagggaatg tagagaccga gctgacgcag tgtttccacc
     5701 agaatcagcg ggctgtgccg ctgcgacccg tcgcggggga acgtcgcatg cgcgcgtggc
     5761 catgacgccg ccgccaggaa ggcgtgcggg gtatcggccg gtcgtacatc cgtaaggagg
     5821 acctctgcca ccgaggtccg gtgcaccagc tcccgctcga cggtacggga ccagctgagc
     5881 ggctcactct ttccgacgac cctgaccgtt gaggtgctca tacgaccagg gaataccgaa
     5941 attcgaacat gcgcaccctt gaattccagg gcgcggggag cggtaggttg ccttccggcg
     6001 gagcgagaac aacgtttgag gtgtacgtgc agatccgggc ggaagcgacg cgcaggttcc
     6061 ttcttgaggc ggcggcaacc ttgttcgacg agaaagggta cgccggtacc agcatcagcg
     6121 acatcagcgc gtattccggt cgcaccagcg gggccatcta ttttcactac gccagcaagg
     6181 agaagctcgc cctcgccatc gtcgaggaac atttcgccac ctggccgcat ttgatcagcc
     6241 gcttccggca gcccggcctg ccggccttgg agaaactcgt cgggctgagc ttcgaggtgg
     6301 cacgcgcctt tcgagatgac atcgtcgtcc gggccggagc ccgcctttgg gccgaacgca
     6361 aagccatcga cgcccagctc cccgcaccgt tcctcggctg gatcgaagcc gttacacagc
     6421 tactcgaaga agccgcccgt aacggtgaac tcgctgccga tgtgccacct gccgccaccg
     6481 cccacggcat cgtctgctcc ttcttcggtc tgcacaccgt ctccgatgcc ctggagggac
     6541 gccggcacat cgaggagcgc ctgcatgacc tgtggactct gctgctccgc ggcctccagg
     6601 ccgagcctga tcccgccggt ctgctcgatc gcgtgcgctg agcggcgcgc gacgcccacc
     6661 gatcttgcag acgttgagga aggccgcagt cggcagctgc gcctgttccc tgttccagtg
     6721 gatgggccgc gaggatcgtc ggggcggtgt ggccgtgcac cagggtgtgc tgtgcggggt
     6781 gcccggtgag agcacccgtg ctgcggcggt cctccgtgcg gtctcacggg ggcgtaccgc
     6841 gtccgcaccg gcctcggcct ggcgctggcg tgcgccatcg acctctcgct tcagcactcc
     6901 tgcggtcggc ggacctcgcc cgtgcccagt gtgatcacgg cccgcggccg catgggctcc
     6961 tcgccgcgtc gggagagcaa gaccacctcc tcgaccaccg ccgacagcgc gccgagccgg
     7021 acggcacact cggcggccag gcgcttcggg tcctgggtgc ggccgaggga caaatgcggg
     7081 gtgaagcggc cggcgcgccc gtggcaaagt gggaaccgca gccccagctc ccggtggaga
     7141 tgggcccacg gcgccgggtc cgccgcagcc ggatcgagcc acaacgtcgc gtagcgccga
     7201 tgccggaaga accgcactcc ggccaggcgc gccgtgaacg ccgtgctctg ggccgccccg
     7261 gcggagagca gcggcacggc ccgcgcaaac gcttcctccg gcacgaaacc gaacagcaga
     7321 ttcacatgcg gcggccaccg ccgtacctgc ggatcgtgtt cccagcggat gtcctgaatc
     7381 accggccaga gcttccgcgg cggcagccac gccactgccg tccgggctgt cggcgccacc
     7441 tcaagagcat ctaccggctc ggccccaccg agatccggat tcccgttcac ctggccatgg
     7501 tcccaaatcg ggctcggaaa accgtccaga gggggccaca gggtgtcgat gtgatcggta
     7561 ccgaggaccc ttgagcggcc ttcaaggacc gccggccgta caggccggcg tccctggcat
     7621 attcgatgca ggcgatggcc gcggcaggcg atggccgcga cggtcacggt ctccgcatcc
     7681 ggaccggggc cccgtccccc gcctgttcaa gcagccggtg cagatgcgcg gtggcgaggt
     7741 gccagaaagg ccggtcggcc gggctgcgag gggcatgtct ggccgcatgc tcgctcacag
     7801 atctggatgc ttcgtcgcca cttggtagta ccccgagtgg aacaccagag gggtgccgcc
     7861 ctcggcctcg tactgttcga cctcgccgag gaagatgatg tggtcgccgg cgtcgaggcg
     7921 ttggacggtg cggcattgga agcgggcgac cgcgccgtcc aggagggggg tgccggcgat
     7981 gccgggggtg gtgggggttc cgcggaattt gtcgtcggcc ggggtggcga actggcggga
     8041 gaggtggtgc tggtcggcgg ccagtatgtg gacggcgaag tgcgaggcgt cggtgaagtc
     8101 cgggaggctc gggctgtttt tgccggggca ccacaggacg aggggcgggt tcatcgagac
     8161 ggaggtgaag gagttcgcgg tcatgccgac gttgcggccg tcgggggagc gggccgtcac
     8221 cacggtcacg ccggtggcgt actggcccag cgcgcggcgc agttcgcggc cgtcgaagcg
     8281 ggcggcgtgc tgggcgcgct tctcggcgag gaagcggtgt gcctcggcct cctcgaacca
     8341 ccagcggtag agggtgcgcg ggtcgtcgaa tccggcggcg agggcgccgg cgacagaggg
     8401 gatttcggcc gccgctgtca gaaggtcacg gtggtgggga ctcaggtcgg ccaggaggga
     8461 gttggtccag ccgacggccc attgagccca gccccgccag aagttgtcga aggtgcgctg
     8521 catccactgc ggggtgaatt cggcggtgcc gtggcggagg atgctgtcga ggtagtgggt
     8581 tgcggcctgg gccgcgttgt tcgaaccctg gccggtgatc ggatcgttga ggacgacggc
     8641 gtcggccatg ccgaggacgt gccgaccgga ggccaggcgg gccacggggc gccggacggt
     8701 cggagtgagc ctgccgcgca acacgccgtt gttatcggtg agtcgggcgt gccggcagcg
     8761 ctcgtattcg tcggggaaga accggtggag gacttccagt gagcgggtga ggtggccttc
     8821 aggcgtgcgg atgtcgtccc agcagtccat cgggccgccg gggacgccct cgaagaccat
     8881 gatgtcgcag ggtccggtgg tggtgagggc cgggaaggag aagtattcgc cgacgccggg
     8941 gaccaagcgg tagtggacgg cgtcctctcc ctcccgcggt gccgttcccg tgacatacgt
     9001 cagagccagc gcacggcgtg gccggtcgta cggggagagt tcggggttgc gcgggaagag
     9061 ctggctcagc tcgcccttgc ccgtggagac cacgacgaga tcgtgcgtac gggcgtacca
     9121 ttcgaggtcg ttgacgcccg cctcgtgcag cacgatctcg ccgcgaccgc cgtccgcgaa
     9181 ctgctctatc caggcggcac acttgacccg ctggtcgacg gagtgggcag gaccctccaa
     9241 cggcgcgcgc caggagacgt ccggagtgcc gtgcggaccg atgagagaga acgcgatgcc
     9301 ggagatgtcc ggggcatggt cctcccagtg gtgcaggccg agttcgcgct cggtctggag
     9361 cgcggtgtcg aacatgcact ggctggacat gaccgggccg cgacgtatct cgtcggggtc
     9421 acggtcggtg accaaggtga cgtcatagcc gtgcgcctgg aggcccaggg ctagttgggc
     9481 acccgcctgc ccggctccga ccaccgcgat ccttctcatg tcctgctgtc ctgctctcgt
     9541 tacgtatcta ttgctgacgt agcgtcagtt cgctggtcct ggcgggccgt tcaggcgccg
     9601 acctcggcca gatagttctt cgcggcgacc gggtccatga accagtggaa caagtcgcgc
     9661 gggtcatcga agccgttgac gaagcgggcg gcgatgcggg agttggcgct cgccgcgccg
     9721 aagagctcca ggacgtgctg tggcggggcg ccgagcatcg cattggtcca ggtcgtggcg
     9781 gcccccgcgt tgtcccagaa gcggccgaag gccagctcca tgaacgcggc gtcgtacggg
     9841 cgttcgcagt ggtccaggat ggtggcgagg taggaggccg cgcacttcga ggcgttgttg
     9901 gacccctggc cggtgatcgg atcgttgagg acgacgacgt cggcgaggcc gagcacctgc
     9961 gcgccggagg gcagcgtggc gacgggcttg cggacggtcg gcgcgaagcg gcccgcgagg
    10021 gtgccgttct cgtcggtgag cgtgacaccg gtgcagcgct cggcctccca cggaacgtac
    10081 gtccgcagca gctcctggaa cttcgccagc tgctggtggg gggtgagccc gtcgaagcag
    10141 tcgagcgggc cacccgggat gccctcgaag aacatgatgt cgcaggggcc gctggtggtg
    10201 aggctgggca tggtgaagta ctcacccaca ccggggatga cgttgaagct gacgccgggg
    10261 cggtcgggca gcgctgccat gccggtgacg tacgccaggg ccagcgcgcg ctgcggagcg
    10321 tcgtacgggg aacgcatggc atcccgctcg aagaggcccg cgatctcgcc cttgccggcc
    10381 gcgacgatca ccaggtcgga ctcggcggcg tagcgctcca ggtcctcgat gccggcctcg
    10441 cagagttcca gacggccgcc gcggcgcgcg aactcgccca tccagagcgg cattttgacg
    10501 cgctggtcga tggagcgggc ggtggcctca aggcgggcgt cgaaggagag cgcccggccg
    10561 ccctggccgt cggcgatgct cacgccgaga ccgtcgatcg acgggcaggc gtcgccccag
    10621 aagtcgaggc cgaggttgcg ctcgtgggcc agcgcggtgc cgaacatggc ctggctggac
    10681 atgacccggc cctcgcggat ctggtctccg gtgcggttgg acacgagggt gacgtcaaaa
    10741 ccgtggtcga tcaggccgat gccgagctgg agacctgcct ggccggctcc gacgatggtg
    10801 atcttgcgca tgagggggac ctccgggggt tggggtgtgc ggttttttga gcagcgccgt
    10861 gcagtgccgt gcgctgtgca ccgtttcggc gcgcggcggc atcgacgagt gggcagcggc
    10921 t
//
