LOCUS       NZ_CP114205            10480 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces tubercidicus strain DSM 40261 chromosome, complete
            genome.
ACCESSION   NZ_CP114205
VERSION     NZ_CP114205.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tubercidicus
  ORGANISM  Streptomyces tubercidicus
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8284047)
  AUTHORS   Ruckert,C., Busche,T., Kalinowski,J. and Wittmann,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-MAR-2022
            Assembly Method                   :: flye v. 2.9; Newbler v. 2.8
            Assembly Name                     :: DSM40261_v1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133.7x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:53:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,167
            CDSs (total)                      :: 7,077
            Genes (coding)                    :: 6,906
            CDSs (with protein)               :: 6,906
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 72 of 171
            Pseudo Genes (incomplete)         :: 133 of 171
            Pseudo Genes (internal stop)      :: 9 of 171
            Pseudo Genes (multiple problems)  :: 41 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:04:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7302839
            Orig. end                         :: 7313319
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114205.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from DSM 40261.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10480
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="27"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10480
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10480
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5480](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5480)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            753..1313
                     /db_xref="GeneID:96287260"
                     /locus_tag="STRTU_RS31940"
                     /old_locus_tag="STRTU_006389"
     CDS             753..1313
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96287260"
                     /gene_functions="regulatory (smcogs) SMCOG1224: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136362.1"
                     /locus_tag="STRTU_RS31940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_006389"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_159748568.1"
                     /transl_table=11
                     /translation="MSPKAMARPGGRSARVQEAVHAAVRELEAEKGRASLTVPAVAARA
                     GVTPSTIYRRWGDLHELLSDVAVERLRPEEPPVDHGSLHTDLESWARQFLDEMASPSGR
                     AYIRDALLGDPDGSNAGQCSAYAADQIEVILARATGRGEAAPDVEAVMDHVVAPMMYRI
                     LFQPGRLTASYADHLVQAALTGP"
     gene            1441..3321
                     /db_xref="GeneID:96287261"
                     /locus_tag="STRTU_RS31945"
                     /old_locus_tag="STRTU_006390"
     CDS             1441..3321
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96287261"
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019887901.1"
                     /locus_tag="STRTU_RS31945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_006390"
                     /product="sensor histidine kinase"
                     /protein_id="WP_246241555.1"
                     /transl_table=11
                     /translation="MERTKRARQVSAVRMRAAFRRLADPGRALVDPGMWPPKRRVLEVL
                     LSVVPAAMAAGNEALHDGGAIRIAAVALAAVLLVPLRRVLPGTVLLVAVAVSAVWGSLA
                     LLIPVASWSAGRRITGVGRATAIFAFTYFLSLVLDFLELPRVSLGLDLLRELSNAAPAT
                     LVFGALATVVAIIVPGLSGRYWSQRRTLLDTLREYNAQLLRERAMIAKQARMRERQRIA
                     QDMHDSLGHQLALISVHTGALEVDRELTGRQREAVGVLREASVGAMHELREVVGLLRDG
                     THAPVDEAPPSPAAADTPTPALEAMGEEDAKAPSRGVAGIERLVAASRSAGTAVELRRT
                     GEVRPLAPTTDHAAYRIAQEGLTNAHKHAPGASIAIELRYEPDSLVVEVANGPMSGSAN
                     DGRGVVSGGQGLTGLGERARLVGGMVHVGATADGGFRLAGVLPYAPPEGGATSPSTSPS
                     SGHETATFVDPAGDLRQQIPAGALGEADLVIDGNGLPKELVKAIRTHKRSSGVAIGCGV
                     AALLALLVAGPVVGGFFLASEADRAVIEPKEYRAMKVGQSEAEVRKRLPSGSSFIAGDL
                     DKGAAPVPEGAQCLSLMSSETSSSWETDPVFRFCFKDGKLIEKTSFEVKT"
     gene            3419..4072
                     /db_xref="GeneID:96287262"
                     /locus_tag="STRTU_RS31950"
                     /old_locus_tag="STRTU_006391"
     CDS             3419..4072
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96287262"
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014154784.1"
                     /locus_tag="STRTU_RS31950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_006391"
                     /product="response regulator transcription factor"
                     /protein_id="WP_159748569.1"
                     /transl_table=11
                     /translation="MIKVLVTDDEPLIRAGIRMILTSADDIDVVAEAANGREAVELARA
                     QRVDVVLLDIQMPVMDGLTALAELRRAAPEARVLILTTFGEQQNVLRALAEGSAGFLLK
                     DSAPAELMRAVRAAAAGDAYLSPGATRHVVDSLASGQSARRSEQARRRLESLSDRELEV
                     LALLGEGLSNADAGQRIHMSEATVKTYVSRILAKLGCENRVQAALLARDAGLGT"
     misc_feature    3466..3481
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.58"
                     /tool="antismash"
     gene            complement(4143..4961)
                     /db_xref="GeneID:96287263"
                     /locus_tag="STRTU_RS31955"
                     /old_locus_tag="STRTU_006392"
     CDS             complement(4143..4961)
                     /EC_number="1.14.18.1"
                     /codon_start=1
                     /db_xref="GeneID:96287263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091617.1"
                     /locus_tag="STRTU_RS31955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_006392"
                     /product="tyrosinase MelC2"
                     /protein_id="WP_159748570.1"
                     /transl_table=11
                     /translation="MAVRKNQADLTATEKRNFVDAVLELKSSGRYDEFINTHNAFIIGD
                     TDNGERTGHRSPSFLPWHRRFLIQFEEALQSITPSVTLPYWDWTVDRTPRSSLWADDFL
                     GGDGRSGDGRVTSGSFAFGSGKWPMNIRVDGRTFLRRSLGTGVRELPTRAEVDQVLAMP
                     VYDAAPWNSSSDGFRNNIEGWRGPNLHNRVHVWVGGQMTTGASPNDPVFWLHHCFIDKL
                     WADWQRLHPKSGYLPTGNTPNVVDLNDTMKPWNDVTPADLLDHTKFYTYA"
     gene            complement(5001..5480)
                     /db_xref="GeneID:96287264"
                     /locus_tag="STRTU_RS31960"
                     /old_locus_tag="STRTU_006393"
     CDS             complement(5001..5480)
                     /codon_start=1
                     /db_xref="GeneID:96287264"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091618.1"
                     /locus_tag="STRTU_RS31960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_006393"
                     /product="apotyrosinase chaperone MelC1"
                     /protein_id="WP_159748571.1"
                     /sec_met_domain="melC (E-value: 6.8e-38, bitscore: 128.7,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRLTRRQVLYSSAAALTGAVLAAPAAFPASAAGRPADGHGHSAG
                     HGHSAGHGHSAGHQMPEPFDEVYQGRHIEGWPADDDGSPDGGHGGHHTGGAAGPHEGMD
                     FVVRIDNDDLHVMRNADGTWISLVNHYETFTTPRALARAAVRELQGAELVPVVTG"
     misc_feature    complement(5954..5967)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 20.06"
                     /tool="antismash"
     misc_feature    5966..5984
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.76"
                     /tool="antismash"
     gene            6040..7578
                     /db_xref="GeneID:96287265"
                     /locus_tag="STRTU_RS31965"
                     /old_locus_tag="STRTU_006394"
     CDS             6040..7578
                     /codon_start=1
                     /db_xref="GeneID:96287265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014046627.1"
                     /locus_tag="STRTU_RS31965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_006394"
                     /product="transcriptional regulator"
                     /protein_id="WP_159748572.1"
                     /transl_table=11
                     /translation="MANEQRIRVGTEIEEFARLLRRLKDRSGRSYGVLAGQLHMSVSTL
                     HRYCNGDAVPMDFAPADRLARRCGATADELVELHRRWILADEARRRSRAAGSSGGTAPG
                     SAASSAPTPSSASTEPSGPAPSSTDEPPALSPDKPPAPSPDKSATSSTDKPATSPTEPT
                     DTTPDPAPDLPSTPPSADEAPGEDAPEVPADPVAVRRTPARRTRLRIALALSAVACLAI
                     PAALAVNDSKGSDSQRRTAGGADRDPGAARADGGKPTRPSASPSPGRSAADGEPTPGGK
                     ARHSPPGHEGPDGSGASRAPEDDRDRGGTAPHVGISSYNWQQPCGVNFLLQKSPDRVPP
                     PPPPQDSRGWARAEGGIDGGHLRLQLTATGRTDAAVVLTSLHVRMVGRHTALPWTAYSM
                     GDGCGGGITPQSFDLDLDDAQPLAKPVAGEDGDVVVPAKNFPFKVSTQDPQVLNLDLHT
                     EGHDVSWYLEVGWSSGGRRGTVRVDDEGKPFRMSAIEGRKHYYYQPDTNHWAPK"
     misc_feature    6556..6558
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7652..8941)
                     /db_xref="GeneID:96287266"
                     /locus_tag="STRTU_RS31970"
                     /old_locus_tag="STRTU_006395"
     CDS             complement(7652..8941)
                     /codon_start=1
                     /db_xref="GeneID:96287266"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1098: enterobactin/ferric enterobactin esterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968393.1"
                     /locus_tag="STRTU_RS31970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_006395"
                     /product="enterochelin esterase domain-containing protein"
                     /protein_id="WP_246241557.1"
                     /transl_table=11
                     /translation="MSRAASFAVTAPADASEPVPYTVASPRLGRFAQRLEQAGTAEHSL
                     LEAEFWAGVERDGTPLIEPLDDDPDHRAVTFLWRGHRATRQVLLLANGLTDPDDLAGSL
                     LAPLPGTEIWYLTHRLRADHRGSYRLVADISPGPLPSAEGPRRARLRGLALHAMPDPHN
                     RTLLPDSAGSAGSSLYELPLSPAQPWREPRPDIPRGAVQCHRSPGATLGDQHTTWVYGP
                     PSGTAGGPLDVLVLPDGERWFGALGLAHTLDALLAEGRIPPLLVLAPDAAGPSGWWQER
                     DTHDAYADFLADDLMSWAAARWPVTAEPRRTVVAGQHLGGTAALHACLRRPERFGGALA
                     QSPAPPGAGRGEPRWPSQWFAPAHRCQARIHLDVARQDVQEAGAESGPALHELLRRCGH
                     TVTSQEFNGGLDYACWRGGLADGLISLFGS"
     misc_feature    complement(8393..8395)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    9215..9235
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.91"
                     /tool="antismash"
     misc_feature    9219..9237
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: strong, score: 32.19"
                     /tool="antismash"
     misc_feature    complement(9626..9640)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.76"
                     /tool="antismash"
     misc_feature    complement(9827..9836)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
ORIGIN
        1 ggagtaccgg ccaactacta cttcgaccac gtcagccccg cggtcgaaat cgctgtgcga
       61 cgtgccctcg cccagatgtc ggacctcggt gccgaactcg tcgagatcga gattccgatg
      121 gcccgctatg ttcaggcgat ccaatggggc ctgatggtcc cggaggccac cgcttaccac
      181 gaacgtaccc tgcggtcggt gcccgagctg tacgcgccgg acgtccgggt gctgctggag
      241 gccggggaac tgatgtcggc cggtgactat ctgcgggccc agcgagcccg taccctcatg
      301 cggcaggcgt ggggacgcgt gctcgaccag gtcgatgtga tcgccgcgcc cacggtgccg
      361 atgacggcag ccgaggccgg tcaggacacc gtggaatggc cggacggtac gacggagagc
      421 gtctcggacg cctatgtccg cctctccgcc cccgcgaaca tcaccggaat cccggcgctt
      481 tccctgccgg tcggccgcga tcacgcgggc ctgcccatcg gcatgcagct gatggggccg
      541 cccagggggg aagccctggt gctgcgcacc ggccatgcct acgaatcggt ccaggagcgg
      601 tgcggtggcc tgccggtcgt gaccgcctga agaatccggc cgtgaccgcc tgaagaatgc
      661 agccccggac tacgcagccc cggactgcgc agccgcggac tacggagagg ggccctgctc
      721 cagacgcaac gattttgctt taaggtggtg gcatgagtcc caaggccatg gctcgccccg
      781 gagggcgcag cgcccgcgtg caggaggcgg tgcacgccgc ggtgcgggaa ctcgaagctg
      841 agaaaggccg cgcctcgctg accgtgccgg ccgtcgcggc ccgcgcgggc gtcaccccgt
      901 cgacgatcta ccgccgctgg ggcgatctcc acgagctgct gtccgacgtg gcggtcgaac
      961 gtctgcggcc cgaggagcca cccgtcgatc acgggagcct gcacacggat ctggagtcct
     1021 gggccaggca gttcctcgat gagatggcct cgccgtcggg acgcgcctat atccgcgatg
     1081 cgctcctggg cgaccccgac ggcagcaatg ccggccagtg ttcggcctac gccgccgacc
     1141 agatcgaggt catcctcgcg cgcgcgacgg gacgcggcga agccgctccc gacgtcgaag
     1201 cggtcatgga ccatgtcgtg gccccgatga tgtaccgcat cctgttccag ccgggccggc
     1261 tcaccgcttc gtatgcggat cacctcgtcc aagccgctct gaccggcccc tgagcaccag
     1321 cacttcacga agcgtccccg caccgcatcg tgggactttc gttgatgtgc ctcgcgacga
     1381 aaggtggtgt cgtcggcgag tagggctgct taacgtggct gcgtgacagg tgtggaaggt
     1441 atggagcgta cgaagcgcgc tcggcaggtg tccgcggtga ggatgcgcgc ggcgttccgg
     1501 cggctggccg atccgggcag ggctctcgtt gatcccggga tgtggcctcc gaagcgcagg
     1561 gtgctggaag tgctgctgag cgtggtgccg gcagcgatgg cggcggggaa cgaagccctg
     1621 cacgacggcg gcgcgatacg gatcgccgcc gttgcgctgg ctgccgtcct gctggtgccg
     1681 ctgcgccggg tgctgcccgg gaccgtactg ctggtggccg tcgcggtctc ggcggtgtgg
     1741 ggcagtctcg ctctgctgat ccccgttgcc agctggtccg ccgggcgccg gatcaccgga
     1801 gtcggcaggg caaccgccat cttcgccttc acctatttcc tcagcctcgt cctggacttt
     1861 ctggagctgc cccgcgtcag cctcggcctg gaccttctgc gggagctgtc caacgccgcc
     1921 ccggcgaccc tggttttcgg cgccctggcc acggtggtcg cgatcatcgt gcccggcctc
     1981 tccggccgct actggtcgca gcgccgcacc ctgctcgaca ccctgcggga gtacaacgcc
     2041 caactgctgc gcgagcgggc gatgatcgcc aaacaggccc ggatgcgcga acgtcagcgc
     2101 atcgcgcagg acatgcacga cagcctcgga catcagctcg ccctcatctc ggtgcacacc
     2161 ggagcgctgg aggtggaccg tgagctgacc gggcggcagc gggaggccgt gggggtgctg
     2221 cgggaggcgt cggtgggtgc gatgcacgag ctgcgcgagg tggtggggct gctgcgggac
     2281 ggcacccacg cccccgtgga cgaagcccct ccctcacctg ctgccgccga cacccccacc
     2341 cctgccctgg aggccatggg ggaggaggac gccaaggcgc cgtcgcgcgg ggtggcgggt
     2401 attgagcgct tggtggcagc ctcccggagc gcgggcacgg ccgtggagct gcggcgcacg
     2461 ggcgaggtgc gcccgctcgc cccgaccacc gatcacgcgg cgtaccggat agcccaggag
     2521 ggcctgacca acgcccataa gcacgcgccg ggcgcctcga tcgccatcga actgcgctat
     2581 gaaccggact cattggtcgt ggaggtcgcg aacggcccca tgtccgggag cgccaacgac
     2641 gggcggggcg tggtgagcgg cggtcagggg ctgaccgggc tcggggagcg ggcccggctc
     2701 gtcggcggca tggtgcatgt gggcgcgacg gcggacggcg gcttccggct ggcgggggtg
     2761 ctgccgtacg ccccgccgga gggcggtgcc accagcccct cgaccagccc ctccagcggc
     2821 cacgagacgg cgacgttcgt tgatccggca ggcgaccttc ggcagcagat tccggcgggc
     2881 gcactcggcg aagctgatct cgtcatcgac gggaacggtc tgccgaagga gctggtcaag
     2941 gccatcagga cgcataagag aagcagtggc gtagccatcg gctgcggggt ggccgcgctc
     3001 ctcgccctgc tggtggccgg ccccgtggtc ggagggttct tcctcgccag cgaagcggat
     3061 cgggccgtta tcgagccgaa ggagtacagg gcgatgaagg tgggccagtc cgaggccgag
     3121 gtgcgcaagc gactacccag cggctcctcg ttcatagccg gagacctcga caagggcgca
     3181 gcgcccgtgc ccgagggggc gcagtgcctc agcctgatgt cctcggagac cagcagtagc
     3241 tgggagacgg acccggtctt ccgattctgc ttcaaggacg ggaagttgat cgagaagacg
     3301 tccttcgagg tcaagacata agggaggaat gctcgccctg aggtccgagc gggcggtgtg
     3361 atcggggatg atggcgagcg gccgcagcta tgaccgttca agagggcgca ggagactcgt
     3421 gatcaaggtt ctcgtcacgg atgacgagcc gctcattcgg gcgggtatca ggatgattct
     3481 cacctcggcc gacgacatcg acgtcgtcgc ggaggcggcg aacggccgcg aggcggtcga
     3541 actggcccgc gcccagcgcg tggacgtcgt actgctcgac atccagatgc cggtgatgga
     3601 cgggctgacg gcgctggccg aactccgccg cgccgccccc gaggcgcggg tgctgatcct
     3661 caccaccttc ggtgagcagc agaacgtgct gcgcgccctg gcggagggca gcgcgggctt
     3721 tctgctcaag gactcggcgc ccgcggaact catgcgtgcg gtacgggccg ccgcggccgg
     3781 agacgcgtat ctgtcaccgg gcgccacccg ccatgtcgtg gactcactgg catccggcca
     3841 gagcgcgcgc cgttccgagc aggcgcgccg caggctggag agcctgtcgg accgtgagct
     3901 ggaggtcctg gcactgctgg gcgagggcct gtccaacgcg gacgccggcc agcggatcca
     3961 tatgagcgag gccacggtca aaacgtatgt gagccgcatc ctggccaagc tgggctgcga
     4021 aaaccgggtg caggccgcac tcctggcccg cgacgccggg ctgggaacct gagacggtgg
     4081 ccgatgcgca cacccgcgct ctcggggtgt gcgcatcggc cgcctgtcat gtcaggtatc
     4141 tgtcaggcat aggtgtagaa cttggtgtgg tccagcaggt ccgccggggt gacgtcgttc
     4201 catggcttca tcgtgtcgtt gaggtcgacg acattggggg tgttgccggt cggcagatac
     4261 cccgacttgg ggtgcagccg ctgccagtcg gcccagagct tgtcgatgaa gcagtggtgc
     4321 agccagaaga ccgggtcgtt gggcgaggcg ccggtggtca tctgcccacc gacccagaca
     4381 tgcacccggt tgtgcaggtt cggaccgcgc cagccctcga tgttgttgcg gaagccgtcg
     4441 gaggagctgt tccagggggc ggcgtcgtag accggcatgg cgagcacctg gtcgacctcg
     4501 gcccgggtcg gcagttcgcg gaccccggtg ccgagtgagc gacgcaggaa cgtccggccg
     4561 tccacccgta tgttcatcgg ccacttgccg ctgccgaagg cgaacgatcc cgaggtcacc
     4621 cggccgtcgc cgctccgccc gtcgccgccg aggaaatcgt cggcccagag ggagctgcgg
     4681 ggggtgcggt caacggtcca gtcccagtac gggagggtga ccgagggcgt gatgctttgc
     4741 agggcctctt cgaactggat caggaacctg cggtgccagg ggaggaagga cggcgaccgg
     4801 tggccggtgc gctccccgtt gtcggtgtcg ccgatgatga aggcgttatg ggtgttgatg
     4861 aactcgtcgt agcggccgct gctcttgagc tccagcaccg catcgacgaa gttccgcttc
     4921 tcggtggcgg tgaggtccgc ctggttcttc cggactgcca tgtcgaggac tcctcggtgg
     4981 ggggacgggc agggcgggtc tcagccggtg acgacgggga ccagttcggc gccctgaagt
     5041 tcacgaacgg cggcgcgcgc caaggcgcgt ggcgtggtga acgtctcgta gtggttcacc
     5101 aggcttatcc aggtgccgtc cgcgttccgc atcacatgga ggtcgtcgtt gtcgatgcgg
     5161 acgacgaagt ccatcccctc gtggggaccg gcggccccgc cggtgtggtg acccccgtgg
     5221 ccgccgtccg gcgagccatc gtcgtccgcc ggccagccct cgatatgacg gccctggtag
     5281 acctcatcga acggctccgg catctggtga ccggctgagt gcccgtgccc ggctgagtgc
     5341 ccgtgcccgg ctgagtgccc gtggccgtcg gccgggcggc cggcagcgct ggcggggaag
     5401 gcggccggtg cggcgagcac cgcaccggtc agggctgcgg cgctgctgta cagcacctgg
     5461 cgtcgggtga gacgcgacat gatccactcc tctgttcgtc tcggctcctc ctctggggcg
     5521 ggcccgcaga gaagaacggt tcttggagcc ggcgcgccga cggtcggccc gcgccgccac
     5581 atgctgacag ggacgaacgg atcatcaagt tgtcccggtg agtgaggcaa cgaacgagac
     5641 aacacccggg cggcagtgga gcattcaacg gcgccaatcg tgcttgcgcc cctccacagc
     5701 cgaccgcgga accggatatt ccgcgatctc ggccgcccct cgccgtagat cctccttccc
     5761 cgcgatcgaa accatgagac cccgcacggc cggaactcgg cgcgaatgca gggggcttgg
     5821 gggaggcggg gtttgcggcg gccttcgccg gtgttgccgt atcggcaacg ccgttgaggg
     5881 gagggtgagt tcgtacggtg tcctcgtgca gtggtgctgc tcacagcctg cgtggcgtgc
     5941 ggtcgggtgc ccgtcgatcc gggacaatga gaccgatccg gaacactgaa acaggtgatg
     6001 acctggggaa atgaccactc cctgacacgc tgttccggaa tggcgaacga acagaggatc
     6061 agggtgggga cggaaataga ggagttcgcg cggctgctgc gccggctgaa ggatcgctcc
     6121 ggacgcagtt atggcgtcct tgccgggcag ttgcatatga gcgtgtccac gctgcaccgg
     6181 tactgcaacg gggatgccgt gccgatggac ttcgcgcccg ccgaccgtct cgccaggcgg
     6241 tgcggggcga cagcggacga actggtggag ctgcaccggc gctggatcct cgccgacgag
     6301 gcccgtcggc gcagccgcgc cgcaggttcc tcggggggca ccgcgccagg ctcggctgcc
     6361 tcgtccgccc cgacgccctc gtccgcctcg accgagccgt ccggcccggc cccctcgtcg
     6421 actgatgagc cgcccgcctt gtcgcccgac aagccgcccg ccccgtcgcc cgacaagtcg
     6481 gccacctcgt cgaccgacaa gccggccacc tcaccaacgg agccgaccga cacaaccccc
     6541 gatccggccc cagatttacc ctccaccccg ccctcagccg acgaagcccc cggcgaggac
     6601 gcgccggaag tgcccgcgga cccggtggca gtcagacgca cccccgcccg gcgcacccgc
     6661 ctccgtatcg ccctcgcgct cagcgcggtg gcatgcctcg ccataccggc cgccctggcc
     6721 gtcaacgact ccaaggggag cgattcccag cggaggacgg cgggcggcgc cgaccgggat
     6781 ccgggcgccg ccagggccga cggtggcaag cccacacggc cgtcggcgag cccttcaccg
     6841 ggccgtagcg cggcggacgg agagcccacc cccgggggaa aggcgcggca cagcccgccc
     6901 ggtcacgagg gccccgacgg ctccggcgcg agccgggccc cggaggacga ccgtgaccgg
     6961 ggaggaacgg ctccgcacgt gggcatcagc tcgtacaact ggcagcagcc ctgtggcgtc
     7021 aacttcctcc tccagaagtc tccggaccgt gtcccgccgc cgcccccacc acaggactcc
     7081 cgcggctggg cacgggccga gggcggtatc gacggtggcc atctgcggct ccagctcacc
     7141 gcaacgggcc gcacggatgc cgccgttgtg ctgacctcgc tgcacgtacg gatggtgggc
     7201 aggcataccg cgctgccctg gaccgcctat tcgatgggcg acggctgcgg cggcggtatc
     7261 accccgcagt ccttcgacct cgatctggac gacgcccagc cgctggcgaa gccggtggcc
     7321 ggagaggacg gcgacgtcgt ggtgcccgcg aagaacttcc cgttcaaggt ctccacccag
     7381 gatccgcagg tcctgaacct cgatctgcac accgaggggc atgacgtcag ctggtatctg
     7441 gaggtcggct ggagcagcgg cggccggcgg ggaacggtcc gggtggacga cgaggggaag
     7501 ccattccgga tgagcgcgat cgaaggccgt aagcactatt actaccagcc ggataccaac
     7561 cactgggctc ccaagtgacc cgaatccccg gagcaacccg agagtccccg agcaagccga
     7621 aagtcctggg ggccgccccg tagtgtcccc gtcagctccc gaacagggag atcagcccgt
     7681 cggccagccc accgcgccaa caggcgtagt cgagaccgcc gttgaactcc tgcgaggtga
     7741 cggtgtgacc acagcggcgc agcagctcat ggagggccgg gcccgactcc gcccctgctt
     7801 cctgcacgtc ctgccgggcg acatcgaggt gtatacgggc ctggcaccgg tgggccgggg
     7861 cgaaccactg cgacggccag cgaggctcgc cacggccggc cccgggggga gcaggggatt
     7921 gggccaaggc gccgccaaac cgctcgggcc ggcgcagaca ggcgtgcagc gccgccgtgc
     7981 cgcccagatg ctgcccggcc acgacggtgc gtcgcggctc cgcggtgacc ggccagcggg
     8041 ccgcagccca ggacatcagg tcgtcggcga ggaaatcggc gtaggcatcg tgggtatcgc
     8101 gctcctgcca ccagccgctg gggccggccg catcgggcgc gagcaccaac agcggcggaa
     8161 tcctcccttc ggcgaggagc gcgtcgagcg tatgggccag gccgagtgcg ccgaaccacc
     8221 gctccccgtc cggcagtacg agaacatcca gggggccgcc cgcagtgccg gacggcggcc
     8281 cgtacaccca ggtggtgtgc tggtcgccga gggtggctcc gggactgcgg tggcactgca
     8341 ccgcgccgcg ggggatatcc ggccggggct ctcgccaggg ttgcgccggg gataacggca
     8401 gctcgtagag cgaactgccg gccgatcccg cactgtccgg cagcagtgtc cggttgtgcg
     8461 ggtcgggcat cgcgtggagg gccagccccc gcaaacgggc gcgccgcggt ccctccgcgc
     8521 tgggcagcgg gccgggtgag atatcggcga ccagccgata ggagccccgg tggtcggccc
     8581 gcaggcgatg ggtcagatac cagatctcgg tgccgggcaa gggggcgagg agggaaccgg
     8641 cgaggtcgtc ggggtcggtg agcccgttgg cgagcagcag aacctggcgg gtggcccgat
     8701 ggccgcgcca gaggaaggtc accgcccggt ggtcgggatc gtcgtcgagc ggctcgatga
     8761 gcggagtgcc gtcccgctcc accccagccc agaactcggc ttccagcaag ctgtgttcgg
     8821 ccgtcccggc ctgttcgagc cgctgggcga accggccgag gcgaggactg gcgacggtgt
     8881 acggcacggg ctcggaggcg tccgcaggcg ccgtgacggc gaaggaggca gcgcggctca
     8941 cctgactgcc ctccgcgtgc actgcggctc accggactgc ggaccggccg tcggccggag
     9001 catggtcccg gtggcggtgg ccgggtatcc cggggtggtt cggagggagg ggcggagggc
     9061 agatcgatac gtcatgtggg gaagggcatt tctgatcgga agggatggtg agtgagcgct
     9121 gagcgctcgg ttcggggcgg tgccgtcagg gccgtcagcg cccgagcggg aaccgatgca
     9181 gcgcgtcctg gccctcggga caccctgccc tcatcaactt agcttaggct tacctaacat
     9241 gcacccggga ttcggccccc agagctcgac ggggcgcccc ccccgggttc cctgaacgcc
     9301 gcctccccgc ccggcagttc gcccacgact gatccacgac tgatccacga ctgatccacg
     9361 cctggtttcg ccatgttcgg tcacgaaccc ttgtgctcgt ttctgttcta cacaacactc
     9421 tcgatgcaca gaaacacgca ctactgaaca tgtcgagtcg tgggcgacga ccatcggtcg
     9481 agtcgaggag tgcacatatg acgccagtca gccgacgtag attcctgagt gcagccatgg
     9541 tgggggccgc gtccatgggg gtcgccatgg gcggcagccg gacggccctc gcgcaggccg
     9601 aggacccgag gggactgacc atccgtggca atgaattcct gctggacggc aagccgttcc
     9661 ggatcctcgc gggcgccttc cactacttca gaacgcaccc caaggactgg cgcgaccggc
     9721 tgacgcgcat gcgggccatg ggcctgaaca cggtggaaac ctatgtcgcc tggaatttcc
     9781 accaaccgta cgagagaaaa gccgacttca ccgggtggcg cgatgtcgcc gccttcgtgc
     9841 gactcgcgga cgaggtcggg ctgaaggtga tcgtgcgacc gggaccgtat atctgcgcgg
     9901 aatgggactt cggcggcctg ccggcctggc tcctcaagga caaggacgcg ccgctacgcc
     9961 gttccgaccc cgctttcgag cgagcggtgg acgcctggtt cgccgaactc ctgccccggt
    10021 tcgtcgatct gcaggccacc cgcggcgggc ccatcatcgc catgcaggtc gagaacgaat
    10081 acggcagcta cggcgacgac cacgcgtatc tggaacatct gcgggacagc atgcgggcac
    10141 agggcatcga cagcctgctg ttctgctcca acggagccag ccaggacgcg ctgaaggccg
    10201 gcagccttcc cgatctcctc tccaccgtca acttcgccgg cgaccccacc ggtcccttcg
    10261 ccgagctgcg cgcattccag ccggaaggtc ccctcttctg cacggaattc tgggacggct
    10321 ggttcgacca ctggggagag ccgcaccaca ccaccgaccc ggcacagacc gccaccgacg
    10381 tggcgaagat gctcgaagcg ggagcgtcga tcaatttcta tatggcggtg ggcggcacga
    10441 acttcggctg gtccgcgggc gccaacctga ccggtagcgc
//
