LOCUS       NZ_CP114205            10417 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces tubercidicus strain DSM 40261 chromosome, complete
            genome.
ACCESSION   NZ_CP114205
VERSION     NZ_CP114205.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tubercidicus
  ORGANISM  Streptomyces tubercidicus
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8284047)
  AUTHORS   Ruckert,C., Busche,T., Kalinowski,J. and Wittmann,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-MAR-2022
            Assembly Method                   :: flye v. 2.9; Newbler v. 2.8
            Assembly Name                     :: DSM40261_v1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133.7x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:53:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,167
            CDSs (total)                      :: 7,077
            Genes (coding)                    :: 6,906
            CDSs (with protein)               :: 6,906
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 72 of 171
            Pseudo Genes (incomplete)         :: 133 of 171
            Pseudo Genes (internal stop)      :: 9 of 171
            Pseudo Genes (multiple problems)  :: 41 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:04:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6326479
            Orig. end                         :: 6336896
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114205.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from DSM 40261.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(643..658)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.75"
                     /tool="antismash"
     misc_feature    658..678
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            687..1919
                     /db_xref="GeneID:96286389"
                     /locus_tag="STRTU_RS27575"
                     /old_locus_tag="STRTU_005514"
     CDS             687..1919
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96286389"
                     /gene_functions="other (smcogs) SMCOG1087: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092346.1"
                     /locus_tag="STRTU_RS27575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005514"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_246241477.1"
                     /transl_table=11
                     /translation="MNKNMNKNTAPRQRALVVGLGVSGIASALRLHKAGWDVVVLEKAA
                     ERRSGGYFIGLFGTGIAAAERLGIELPDRLSADIATYNVDRADRRRRAMNFTDAVPGRA
                     RLVVRGDIEDGAFHALPEDVEIRYSSVPVALRQDDDGVEVDVEDRAAATATTERFDLVV
                     GADGLRSTVRRLAFGPDEGRIHRLGYMIAACSLPGPAPGYRLKEGIIMAEQGRSAWVFP
                     FADRAPTVLFNYRTDDVDAEFTRPAIDSLRARFGPEPTGGTLGWLLDRFEQAPDHLFDS
                     AEQVRLDRWHQDRVVLVGDAAWCLTLYSGMGASTGLAGADMLGTMLERYPGEVPRALRA
                     WEAKLRPFIEYHQGTGMAMRRLFVPADPTEYRIRSVMNRVMATRPGREFLSKRRHRSES
                     SRMKTIDIALA"
     gene            2011..2730
                     /db_xref="GeneID:96286390"
                     /locus_tag="STRTU_RS27580"
                     /old_locus_tag="STRTU_005515"
     CDS             2011..2730
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96286390"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="STRTU_RS27580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005515"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_159747337.1"
                     /transl_table=11
                     /translation="MSRLIEHDSAKAARILDSARDLVVEHGVRKVTIAEIAAAAGVGKG
                     TVYLYWETKEDLFVGLAAREVLGWIDTVTTRIGQDPHTVLPRHLAPLLIRTTHGNPWLR
                     WLRSDDSALRQLLHRPADQERFADASPSAMGNAVITILRDHGIVRDDLPLARQMYALHA
                     VLVGFGNVMAHPAGAGPLNVDDPETALADTVHLLLEKPRDPAARVVAQAAEAVRARFTE
                     IHDSLLELVAAGAAGTR"
     gene            2845..3906
                     /db_xref="GeneID:96286391"
                     /locus_tag="STRTU_RS27585"
                     /old_locus_tag="STRTU_005516"
     CDS             2845..3906
                     /NRPS_PKS="Domain: Aminotran_5 (17-348). E-value: 2e-21.
                     Score: 68.3. Matches aSDomain:
                     nrpspksdomains_STRTU_RS27585_Aminotran_5.1"
                     /codon_start=1
                     /db_xref="GeneID:96286391"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606338.1"
                     /locus_tag="STRTU_RS27585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005516"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_159747338.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.7e-15, bitscore:
                     53.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHRREPLGGAEFAPETTYLNSASSGLLPARSAVALRAALDESASY
                     GTMGRDYFGAAAASREAFARLLGVPAERVAVGSSVAVQSAFVASALPAGAEVLAPEGDF
                     SSLLNPLAAHPGIKLRTVPLEALADAVRPGTALVAFSAVQSLDGRIADMAAVREAARAH
                     GARTYLDITQAAGWLPLRADGFDFVVAGAFKWLLCPRGVTFMAFGGEYGRGEEWPAAPY
                     AGWVAGEDIGASNYGLVRPAATARRFDEPHAHYSYVGAEQSLGLLNELGVETVHGHNTA
                     LAEYYRAGLAERGLTPRAAPGSAIVSTPGIADAEHRLAEAGVRISVRGGLLRAAFHLYN
                     STDDVDRLLALVG"
     aSDomain        2896..3888
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRTU_RS27585_Aminotran_5.1"
                     /evalue="2.00E-21"
                     /label="STRTU_RS27585_Aminotran_5.1"
                     /locus_tag="STRTU_RS27585"
                     /protein_end="348"
                     /protein_start="17"
                     /score="68.3"
                     /tool="antismash"
                     /translation="YLNSASSGLLPARSAVALRAALDESASYGTMGRDYFGAAAASREA
                     FARLLGVPAERVAVGSSVAVQSAFVASALPAGAEVLAPEGDFSSLLNPLAAHPGIKLRT
                     VPLEALADAVRPGTALVAFSAVQSLDGRIADMAAVREAARAHGARTYLDITQAAGWLPL
                     RADGFDFVVAGAFKWLLCPRGVTFMAFGGEYGRGEEWPAAPYAGWVAGEDIGASNYGLV
                     RPAATARRFDEPHAHYSYVGAEQSLGLLNELGVETVHGHNTALAEYYRAGLAERGLTPR
                     AAPGSAIVSTPGIADAEHRLAEAGVRISVRGGLLRAAFHLYNSTDDVDRL"
     gene            complement(4007..4906)
                     /db_xref="GeneID:96286392"
                     /gene="thpD"
                     /locus_tag="STRTU_RS27590"
                     /old_locus_tag="STRTU_005517"
     CDS             complement(4007..4906)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /db_xref="GeneID:96286392"
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="STRTU_RS27590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005517"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_159747339.1"
                     /sec_met_domain="PhyH (E-value: 1.8e-36, bitscore: 125.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTAEVITPRKDPVVWSQPGTAGPFEPSELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVLDPTMRADPRSIVEPKSQDVRTVFEVHKI
                     SEVFAKLVSDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYTTNGGLMIMPGSHQHFVGCEGATPKDNYKRSLQMQDAGIPSDEV
                     LTKMADKHGIRLFTGQAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENEAVEPFA
                     APIRRPEYIGARDFTPVR"
     gene            complement(5001..5417)
                     /db_xref="GeneID:96286393"
                     /locus_tag="STRTU_RS27595"
                     /old_locus_tag="STRTU_005518"
     CDS             complement(5001..5417)
                     /codon_start=1
                     /db_xref="GeneID:96286393"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606340.1"
                     /locus_tag="STRTU_RS27595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005518"
                     /product="ectoine synthase"
                     /protein_id="WP_018091828.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.8e-53, bitscore:
                     178.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKYTITPGTMYLLDGHEKHTMRIKEDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPESEPETV"
     gene            complement(5464..6732)
                     /db_xref="GeneID:96286394"
                     /gene="ectB"
                     /locus_tag="STRTU_RS27600"
                     /old_locus_tag="STRTU_005519"
     CDS             complement(5464..6732)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 6e-76.
                     Score: 247.6. Matches aSDomain:
                     nrpspksdomains_STRTU_RS27600_Aminotran_3.1"
                     /codon_start=1
                     /db_xref="GeneID:96286394"
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="STRTU_RS27600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005519"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_088797091.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.5e-79, bitscore:
                     263.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRSWPTVFDRAQGSRMTDEDGHTY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGVTHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGTYPDFLWFERLLEDQGSGLNTPAAVIVETVQGEGGINVARP
                     EWLRALADLCERWDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAATLDTYWADGQMEKQTLARGEIVEAHLK
                     AIAEEHPDAIAEYRGRGLVWGMELNDKPVADKIAKRAFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRILARAVRDCV"
     aSDomain        complement(5638..6648)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRTU_RS27600_Aminotran_3.1"
                     /evalue="6.00E-76"
                     /label="STRTU_RS27600_Aminotran_3.1"
                     /locus_tag="STRTU_RS27600"
                     /protein_end="365"
                     /protein_start="28"
                     /score="247.6"
                     /tool="antismash"
                     /translation="FDRAQGSRMTDEDGHTYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGTYPDFLWFER
                     LLEDQGSGLNTPAAVIVETVQGEGGINVARPEWLRALADLCERWDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAATLDTYWADGQMEKQTLARGEIVEAHLKAIAEEHPDAIAEYRGRGLVWGMELN"
     gene            complement(6869..7435)
                     /db_xref="GeneID:96286395"
                     /gene="ectA"
                     /locus_tag="STRTU_RS27605"
                     /old_locus_tag="STRTU_005520"
     CDS             complement(6869..7435)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96286395"
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577188.1"
                     /locus_tag="STRTU_RS27605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005520"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_159747340.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSDIREFPEGFKLDTPRVEDGAAIWRIARDSKTL
                     DLNSSYSYLLWCRDFAATSVVARDAKGEPAAFITGYLRPERPETLVVWQVAVDDAHRGQ
                     GLAAALLDGLTARARDELGVRFVETTVTPDNTASNRLFASFAERHSVPLRSEVLFDAGL
                     FPEQGHEPEVLHLIGPFEPPARPGH"
     misc_feature    7591..7606
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    7601..7616
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7620..7638)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.12"
                     /tool="antismash"
     gene            8047..9138
                     /db_xref="GeneID:96286396"
                     /locus_tag="STRTU_RS27610"
                     /old_locus_tag="STRTU_005521"
     CDS             8047..9138
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96286396"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="STRTU_RS27610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005521"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_159749617.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.2e-16, bitscore:
                     58.7, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEGAVLHLKGRVLVGPDDVRDELWVVDGRVTFDRPVMARDATTV
                     TGWALPGLVDAHCHVGLDAHGPVDAPTSEKQALTERDAGTLLLRDAGSPSDTRWVDDRE
                     DLPRIIRAGRHIARTKRYIRNYAHEIEPEDLVAYVAQEARRGDGWVKLVGDWIDRSTGD
                     LAACWPPGAVKAAIAEAHRLGARVTAHCFAEDTLAPLVEAGIDCIEHATGLTEDTIPLF
                     AERGVAIVPTLVNIATFPQLADGGAAKFPRWSDHMRRLHARRYDTVRAAYDAGVPVFAG
                     TDAGGSLAHGLVAEEVVELTRAGLPAVAALSATTWGARDWLGRPGLTEGASADLVVYES
                     DPREDVRVLAAPRRVVLRGRIVG"
     gene            9437..10408
                     /db_xref="GeneID:96286397"
                     /locus_tag="STRTU_RS27615"
                     /old_locus_tag="STRTU_005522"
     CDS             9437..10408
                     /codon_start=1
                     /db_xref="GeneID:96286397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536882.1"
                     /locus_tag="STRTU_RS27615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_005522"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_167539297.1"
                     /transl_table=11
                     /translation="MPRRLAATLVATALTAGPAVLLGAVPAHATGDHGPARGTADAVVL
                     RTGVNVGLLNKTLDVPLNVALNEVHAPASANKTALTVKLDGIDHGRPFSVLRADAATAR
                     ATADRRAAEGYANLVRAKVRLPGLPLLSVIEVQQVTAKATCEAGARPRAEANVLGTVRV
                     LGKKVTLSTGGTTKVKVPGTGEVRLDLSKKSVTSKGAAATALDLNVSLNPLKLNVADVQ
                     GRVTLAKASCTTPRGKAPEDNATSGGQTGGGEIGGTEPAGGKGTEVKPANSGPRTQNLA
                     ETGGSSATPYLAGGAGLLVAAGAGAMVFARRRRSAAPQGGRG"
ORIGIN
        1 gcgagcaggg cgaggcccgg ccgctgcgcc gcgcgttcga ggttccggcc cagctcggcc
       61 atcaccggtt gggcggcgga gcggtagagc gcgtgcgcgg cccgccccat cgcctcgtcc
      121 tggccctccg cgacccgcct ggccaccggc tcgctcatgc cgagcccgac gagcttcgag
      181 acccgcgccg cgaggtccgc accgaacggg atggccgggt ccggtaccgc gctctccggc
      241 ttctgctgcc gctgcgcgaa cgcgtgccat acgtagtccg ggtcgaacag gccgatcaca
      301 tcgctgaccc agctgcgcac gaggtccggg cgggccatca ccgcattgag ggtgtgcgcg
      361 ccgccccagt cgtgcccgac caggtcgacg ggctcggtga accgctccaa ttcgccgatg
      421 agccagtccc ggtagtcacc gacggccgcg ccgaaccccc tcggcacagg tgccccgaag
      481 ccgggcggcg acaggcagat cgggccgggg cgagcgggag cgatgcgttc gagctcggcg
      541 agcagcgggt cccagaccgc cgccgtctcg gggtggccgt ggacgaaaac cgtgggcatg
      601 gcgggaactc cgttccgatg agtcgttcgc cacaggagcg aactgaccag atggctattc
      661 tggtcagtga gtctactctc ggcgtcatga acaagaacat gaacaagaac acagccccgc
      721 gacagcgggc tctcgtagtg ggactcggcg tgagcggaat cgcctcagcg ctgcggctgc
      781 acaaggcggg ctgggacgtc gtcgtcctgg agaaggccgc cgagcgccgc agcggcgggt
      841 acttcatcgg cctcttcggc accgggatcg ccgcggccga gcgcctgggt atcgagctgc
      901 cggaccgcct ctcggcggac atcgcgacct acaacgtcga ccgcgcggac cggcggcggc
      961 gtgccatgaa cttcaccgac gcggtccccg gccgggcacg cctggtcgtg cgcggcgaca
     1021 tcgaggacgg cgccttccac gccctgcccg aggacgtgga gatccggtac tcgtcggttc
     1081 cggtcgcgct gcgccaggac gacgacggcg tggaggtcga tgtcgaggac cgggccgcgg
     1141 cgacggcgac caccgagcgg ttcgacctcg tggtcggggc cgacggcctg cgctcgacgg
     1201 tgcgccggct ggccttcggg cccgatgagg gccgcatcca ccgcctcggc tacatgatcg
     1261 cggcgtgcag cctgcccggc ccggcccccg gctaccggct caaggaaggc atcatcatgg
     1321 ccgagcaggg ccggtcggca tgggttttcc cgttcgccga ccgggcgccg acggtgctgt
     1381 tcaactaccg gaccgacgac gtggacgccg agttcacccg gcccgccatc gactcgctgc
     1441 gggcgaggtt cggaccggag cccacgggtg ggacgctcgg ctggctgctc gaccggttcg
     1501 agcaggcgcc cgaccacctc ttcgacagcg ccgagcaggt gcggctggac cggtggcacc
     1561 aggacagggt ggtgctcgtc ggcgacgcgg cgtggtgcct gacgctctac tccgggatgg
     1621 gggcctccac cggcctggcc ggggccgaca tgctgggcac gatgctggag cggtaccccg
     1681 gagaggtgcc gcgggcgctg cgggcctggg aagcgaagct ccgcccgttc atcgagtacc
     1741 accagggcac cggcatggcc atgcgcaggc tcttcgtccc ggccgacccc accgaatacc
     1801 ggatccggtc cgtcatgaac cgcgtgatgg ccacgaggcc cggcagggag ttcctctcga
     1861 agcggcggca ccgcagcgag tcctcccgga tgaagacgat cgacatcgcc ctggcgtgac
     1921 ccggcgcggg ggcctgccgc tcagccggct cacctctgtg gactcccgtg ccatcatggt
     1981 cacgaagagc gaacgggagg aagcacgacg gtgagccggc tgatcgaaca cgactccgcc
     2041 aaggcggcaa ggatcctcga cagcgcgcgc gatctggtcg tcgaacacgg tgtccgcaag
     2101 gtgaccatcg ccgagatcgc cgcggccgcg ggcgtgggca agggcaccgt ctacctgtac
     2161 tgggagacca aggaagacct gttcgtgggg ctggccgcgc gcgaggtgct gggctggatc
     2221 gacaccgtca ccacccggat cggccaggac ccgcacaccg tcctgccccg gcacctcgcc
     2281 ccgctgctga tccgcacgac acacggcaac ccgtggctgc ggtggctccg gagcgacgac
     2341 agcgccctgc gccagctgct ccaccgcccc gccgaccaag agcgcttcgc cgacgcctcc
     2401 ccgagcgcga tgggcaacgc ggtcatcacg atcctgcgcg accacgggat cgtgcgcgac
     2461 gatctcccgc tggcccggca gatgtacgcc ctgcacgccg tgctggtcgg gttcggcaac
     2521 gtcatggccc accccgcagg cgcaggcccg ctcaacgtcg acgatcccga aaccgccctc
     2581 gccgacaccg tccacctgct gctcgaaaag cctcgcgacc ccgccgcgcg cgtggtcgcc
     2641 caggccgcgg aggcggtgcg ggcccggttc accgagatcc acgacagtct cctggaactg
     2701 gttgcggcgg gcgctgccgg aaccaggtga cgagcgcggg gccggcccgc cgcatcccct
     2761 gcggcctgcg ccgacccgga aggcccgcgg ccggaaacgg cacgcgtcat ggacgggccg
     2821 acgctcaccg gccacagtgg gggcatgcat cgacgtgagc cactcggtgg ggccgaattc
     2881 gcgcccgaga cgacatacct gaactctgcc tccagcggac tcctcccggc acgcagcgcg
     2941 gtcgccctgc gcgcggccct cgacgaatcc gcttcctacg gcaccatggg ccgcgactac
     3001 ttcggcgccg ccgcggcgtc gcgggaggcc ttcgcccggc tgctgggcgt gcccgccgag
     3061 cgggtcgccg tcggcagctc ggtcgcggtg cagtccgcgt tcgtggcctc cgcgctcccg
     3121 gcgggcgccg aagtgctggc gcccgagggc gacttcagct cgctgctcaa tccgctcgcc
     3181 gcccacccgg gcatcaagct gcgcacggta ccgctggagg cgctcgccga cgcggtgcgg
     3241 cccgggaccg cgctggtcgc gttcagcgcc gtgcagtcac tggacggccg gatcgccgat
     3301 atggcggcgg tccgtgaggc cgcgcgggcc catggggcgc gcacctacct cgacatcacc
     3361 caggccgccg gctggctgcc gctgcgcgcc gacggcttcg acttcgtcgt cgcgggggcg
     3421 ttcaagtggc tgctgtgccc gcgcggggtg acgttcatgg cgttcggcgg ggagtacggg
     3481 cggggcgagg agtggccggc cgcgccgtat gcgggatggg tcgcggggga ggacatcggg
     3541 gcgtccaact acgggttggt ccggcccgct gccaccgcac ggcgcttcga cgaaccgcat
     3601 gcccactact cctacgtggg tgccgagcag tccctcggcc ttctcaacga gctgggtgtg
     3661 gagaccgtcc acggccataa caccgcgctc gccgagtact accgcgcggg gctggccgag
     3721 cgcggtctga caccgcgggc cgcgcccggt tcggcgatcg tctccacccc ggggatcgcg
     3781 gacgcggagc accggctggc cgaggcgggc gtgcggatct cggtgcgcgg cgggctgctc
     3841 cgcgcggcct tccacctcta caactcgacg gatgatgtgg accggttgct ggctctggtg
     3901 gggtagggcg acccgcaccg gccgtacgca cccgccccca ccaacaacgg cgcggccgcc
     3961 gcacagactc gctgtgcggc ggccgcgcca ccggtgcgaa cgtcccctaa cgcaccggag
     4021 tgaagtcccg ggccccgatg tactcggggc gccggatggg tgccgcgaac ggctccaccg
     4081 cttcgttctc cacgctgttg aagacgatga agacattgct gcgcgggtag ggcgtgatgt
     4141 tgtcacccga gccgtgcatg gcgttgcagt cgaaccacgt cgccgaaccg gcctgaccgg
     4201 tgaagagacg gatgccgtgc ttgtccgcca tcttcgtcag cacctcgtcc gacgggatgc
     4261 ccgcgtcctg catctgcagc gaccgcttgt agttgtcctt cggcgtcgca ccctcgcagc
     4321 ccacgaagtg ctggtgcgag ccgggcatga tcatcaagcc gccgttggtg gtgtagttct
     4381 ccgtcagcgc gatcgagacc gacacggtgc gcatattcgg cagaccgtcc tcggcgtgcc
     4441 aggtctcgaa gtccgagtgc cagtagaagc cggaggcacc gaaacccggc ttcacattga
     4501 tccgcgactg gtggacatag acgtccgaac cgaggatctg acgggcacgg ccgaccacac
     4561 gcgggtcgga gaccagcttg gcgaacacct cgctgatctt gtgcacctcg aacacggtcc
     4621 gtacgtcctg tgacttcggc tcgacgatgg agcgcgggtc ggcgcgcatc gtcgggtcga
     4681 ggacgagacg gtccagttcg gcgcggtaca ccgcgacttc ttccgccgtg agcagttccc
     4741 cgatggcgaa aaagccgtca cgttcgaagt cgctcagctc ggacggctcg aaggggccgg
     4801 ccgttccggg ctgcgaccac accaccgggt ccttccgcgg ggtgatgacc tcagcggtcc
     4861 cacgggtcgg gtacaggtcg gcggtgcgct cgggtgcggt ggtcatggtg ttgccttcct
     4921 ctcctctcgt acggaatctg gcttacgtgc gcgtacgtgc cttcctcagc gctgccggac
     4981 ggggcaggcg gggtggcgcc tcagaccgtc tcgggctcag actccggttc ggtgagaagc
     5041 gggtagaccc cgttctcgtc gtggtcctcg cggccggtga cgggcgggtt gaagacgcag
     5101 acgcagcgga agtcttcctt gatccgcatg gtgtgcttct cgtgcccgtc gagcaggtac
     5161 atcgtgcccg gcgtgatggt gtatttctcg ccggtctcgt cgttcgtgag ctcggcctca
     5221 ccctctacgc agagtacggc ctcgatgtgg ttcgcgtacc acatcgacgt ctcggtcccg
     5281 gcgtacagcg tggtctcgtg cagcgagaag ccgacgcgct ccttggcgag cacgatgcgc
     5341 ttgctctccc aggtgccgga cttggccttg acgtggcggt cggtgccttc gatgtccttg
     5401 aaggatcgga cgatcacggt gactcggtgc ctttctgtgg aacggtaagg ggctctggag
     5461 tggtcagacg cagtcgcgga ccgcgcgggc gaggatccga aggccctcgt ccagctcctc
     5521 gggggtggtc gtcagcgcgg gcagcagctt gacgacctcg ctctccgggc cggaggtctc
     5581 gatcagcaga cccagctcga aggcgcgctt ggcaatcttg tcggcgacgg gcttgtcgtt
     5641 gagctccatg ccccacacca gaccgcggcc gcggtactcg gcgatggcgt ccgggtgctc
     5701 ctcggcaatg gccttgaggt gtgcttcgac gatctcaccg cgggcgagag tctgcttctc
     5761 catctggccg tcggcccagt aggtgtccag cgtcgcggcg gcggtgacga acgccgggtt
     5821 gttgccacgg aaggtgccgt tgtgctcgcc cggctcccag atgtccagct caggcttgaa
     5881 cagggtgagc gcgagcggca ggccgtagcc gctgatggac ttcgagacgg tgacgatgtc
     5941 cggcacgatg cccgcctcct cgaaggagaa gaaggcaccc gtacggccgc agcccatctg
     6001 gatgtcgtcg acgatgagca gcatgtccca gcgctcgcac aggtcggcga gggcgcgcag
     6061 ccactcggga cgggcgacgt tgatgccgcc ctcgccctgg accgtctcga cgatcacggc
     6121 ggcgggggtg ttcaggccgg agccctggtc ctccagcagc cgctcgaacc acaggaagtc
     6181 cgggtaggtg ccgtcgaggt agttgtcgaa cggcatcggg gtgccgtgca ccagcgggat
     6241 accggcaccg gcccgcttca tggagttacc ggtgacggcg agcgcgccca gcgacatgcc
     6301 gtggaaggcg ttggtgaagg acacgatcga ctcgcggccc ttgaccttac gggccagctt
     6361 cagcgcggcc tcgacggagt tggcgcccgt cgggcccggg aacatgacct tgtagggcag
     6421 gtcacggggc cgcaggatga tgttctggaa cgactccagg aaggcccgct tggccgtggt
     6481 ggacatgtcc aggccgtggg tgacgccgtc ccgctcgatg tagtcgatca gggcgcgttt
     6541 gaggacgggg ttgttgtggc cgtagttgag cgacccggcc ccggcgaaga agtcgaggta
     6601 ggtgtggccg tcctcgtcgg tcatgcggct gccctgtgca cggtcgaaga cggtgggcca
     6661 gctacggcag tagctgcgga cctccgactc cacggtctcg aagacgctca gatcgggctg
     6721 ggtgatggtc acagcatgct cctgggagat gagtggcgaa aggtgaggaa gtctgttggg
     6781 ggctgggcgg taccggcggt gtcacggggt gtggtgccgg gtcccgcacg gccgctgcgg
     6841 cgtcgtcgcc gcgcggtcct gggtggcgtc agtgccccgg gcgggccggt ggctcgaacg
     6901 ggccgatgag gtgcagcacc tccggctcgt ggccctgctc cgggaacagc cctgcgtcaa
     6961 agagcacctc gctcctgagc ggcactgagt gccgctcggc gaaggacgcg aacaaccggt
     7021 tggatgcggt gttgtcgggg gtgacggtgg tctcgacgaa acgtacgccc agctcgtccc
     7081 tggcgcgggc ggtgagcccg tccaggagtg cggccgccag tccctgccca cggtgcgcgt
     7141 cgtcgacggc gacctgccag acgacgaggg tctccgggcg ctccgggcgg agatatccgg
     7201 tgatgaaggc ggccggttca cccttcgcgt cgcgggcgac gacagaggtt gcggcgaagt
     7261 cgcgacacca caggaggtag ctgtacgagg agttgagatc cagcgtcttc gagtcgcggg
     7321 cgatgcgcca gatcgcggct ccgtcctcca cgcgtggggt gtcgagcttg aagccctccg
     7381 gaaattctct gatatcgctt cgggcaccgg catggtctgc ttgtgcggcg gtcatgggaa
     7441 ttaaatttac ccagggaaaa aggaaattgc atcgcgggca ggggttacgg aaccggcgaa
     7501 gatgtgttat cgcgcggggg cgcccgtacg cgcgatgcca ccgcgagatg ccccgatttg
     7561 accgggatgt tcggggcaaa acggacacgt tgtgtagtcg gtcacagctc cgtaacgcct
     7621 atgagatccg ctcgaattat gggacttggc actagcgaaa ccttggcgtt tgagctgcgg
     7681 aaaagcgggc agaagaatac gggaagctgt gctcccaggg aattgggaat tcgccgccgg
     7741 aaggtcacgc ggaaggccca tggaatcctg cgcggcaaga ccgtgacaag acccccgaga
     7801 acttgcgcga ggcgatctcc gggaagagtg tgtgaaacct gcggagagtt gcggagaacg
     7861 ctcggagaac cgtgccgcgt acgtggcgga agccctacgg agagtgcgcg gaaagcggtc
     7921 cgtacggaaa gtccgccaca gtgcccgatc ccgggaaatc ggcgggggga aagagggttg
     7981 ttcaacgggc gcgaccgccc cggccgcccg ccgtggtgcg gcgcctacag tgccggtatg
     8041 acgtccatga gtgaaggtgc ggtgctgcac ctcaagggga gggtcctcgt cggaccggac
     8101 gacgtgcgcg acgagctgtg ggtggtcgac ggccgggtga cgttcgaccg gccggtgatg
     8161 gcacgggatg ccaccacggt gacgggctgg gccctgccgg gcctggtcga cgcgcactgc
     8221 catgtgggcc tggacgcaca cggcccggtg gacgccccca ccagcgaaaa acaggcgctc
     8281 accgagcgtg acgccggcac cctgctgctc cgcgacgcag gatccccctc cgacacccgc
     8341 tgggtcgacg accgcgagga tctgccccgg atcatccggg ccggccgcca catcgcccgt
     8401 accaagcgct atatccgcaa ctacgcgcac gagatcgagc cggaggacct ggtcgcctac
     8461 gtcgcccagg aggcccgccg cggcgacggc tgggtcaagc tcgtcggcga ctggatcgac
     8521 cgctccacgg gcgatctggc ggcctgctgg cccccggggg cggtgaaggc ggccatcgcc
     8581 gaggcgcacc ggctgggcgc ccgggtgacc gcccactgct tcgccgagga caccctcgcc
     8641 ccgctcgtcg aggccggaat cgactgcatc gagcacgcca ccgggctcac cgaggacacc
     8701 atcccgctgt tcgccgagcg cggagtggcc atcgtcccca cgctcgtcaa catcgccacc
     8761 ttcccgcagc tcgcggacgg cggcgcggcg aagtttccgc gctggtcgga ccatatgcgg
     8821 cggctgcacg cccgccgcta cgacaccgtc cgggcggcct acgacgccgg agtgcccgtc
     8881 ttcgccggta cggacgccgg cggctcgctc gcccacgggc tggtggccga ggaggtcgtc
     8941 gagctgaccc gggccggtct gcccgccgtg gcggccctct ccgccaccac ctggggcgcc
     9001 cgggactggc tcggccgccc cggcctcacc gagggcgcct cggcggacct ggtggtctac
     9061 gagagcgatc cgcgcgagga cgtccgggtg ctggccgcgc cccgccgggt ggtgctgcgg
     9121 gggcggatcg tcgggtagcc gcggggcggc cgccggggtg gtcccgtgaa ccggcccggt
     9181 caaccgggat cacccgggag gtggggcggc tgtggcgcgg gtggtgcccc gtgtcgcggg
     9241 aatcgaatac aggcacggaa accacccatt gggatgaact gacgccacgt ggctgcccgt
     9301 tcaccctcgg tgcggacacg atgtgcgggt cgatgccgtc ggcccgcgcg atgtccccca
     9361 ttggcgcggc cggcggctcc atttctcctg tgagggttcc acacacgtgt ccaccagtcc
     9421 caccagctcc ttcggcatgc cccgacgcct cgccgcgacc ctcgtcgcca ccgcgctcac
     9481 ggccggtccc gccgtcctgc tcggcgccgt ccccgcgcat gccaccggag accatggccc
     9541 ggcccgcggc accgccgacg cggtcgtgct gcgcaccggc gtgaacgtcg ggctgctcaa
     9601 caagacgctc gacgtcccgc tgaacgtcgc cctcaacgag gtgcacgccc cggcctcggc
     9661 caacaagacc gcgctcaccg tcaagctcga cggcatcgac cacggcaggc cgttcagcgt
     9721 gctgcgcgcc gacgccgcca ccgcacgggc caccgccgac cgccgggccg ccgagggcta
     9781 cgccaacctc gtgcgggcga aggtgcggct gcccggtctg ccgctgctgt ccgtgatcga
     9841 ggtccagcag gtcaccgcca aggcgacctg tgaggcgggc gcgcggcccc gcgccgaggc
     9901 gaatgtgctg ggcaccgtgc gcgtcctggg caagaaggtc acgctcagca cgggcggcac
     9961 gacgaaggtc aaggtgccgg ggaccggcga ggtgcggctg gacctgtcca agaagagcgt
    10021 cacgtccaag ggcgccgcgg ccaccgccct cgacctcaat gtctccctca acccgctcaa
    10081 gctgaacgtc gccgatgtgc agggccgggt caccctggcc aaggcaagct gcaccacacc
    10141 gcgcggcaag gcgcccgagg acaacgccac cagcggcggc cagaccggcg gcggcgagat
    10201 cggcggcacc gagccggccg ggggcaaggg caccgaggtg aaaccggcga actccgggcc
    10261 gcgcacccag aacctcgcgg agaccggcgg cagctcggcc accccgtacc tcgccggcgg
    10321 cgccgggctg ctggtcgccg cgggcgccgg tgccatggtc ttcgcccgcc ggcgccgcag
    10381 cgccgcaccg cagggcggcc gcggctgacc gaccccg
//
