LOCUS       NZ_CP114205            10978 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces tubercidicus strain DSM 40261 chromosome, complete
            genome.
ACCESSION   NZ_CP114205
VERSION     NZ_CP114205.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tubercidicus
  ORGANISM  Streptomyces tubercidicus
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8284047)
  AUTHORS   Ruckert,C., Busche,T., Kalinowski,J. and Wittmann,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-MAR-2022
            Assembly Method                   :: flye v. 2.9; Newbler v. 2.8
            Assembly Name                     :: DSM40261_v1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133.7x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:53:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,167
            CDSs (total)                      :: 7,077
            Genes (coding)                    :: 6,906
            CDSs (with protein)               :: 6,906
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 72 of 171
            Pseudo Genes (incomplete)         :: 133 of 171
            Pseudo Genes (internal stop)      :: 9 of 171
            Pseudo Genes (multiple problems)  :: 41 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:04:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1841861
            Orig. end                         :: 1852839
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114205.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from DSM 40261.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10978
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="13"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10978
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10978
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5978](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5978
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            251..1114
                     /db_xref="GeneID:96282466"
                     /locus_tag="STRTU_RS07885"
                     /old_locus_tag="STRTU_001576"
     CDS             251..1114
                     /codon_start=1
                     /db_xref="GeneID:96282466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088832.1"
                     /locus_tag="STRTU_RS07885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001576"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_159742891.1"
                     /transl_table=11
                     /translation="MNDQQTQRTPRSLIVTFYGAYGRGGPGAPGGPAAPVPVAALIRLL
                     GALGVDPPSVRSAVSRLKRRGLLAPARTATGAAAYGLSDAARQLLDDGDRRIYARPAAR
                     LSDGWVLAVFSVPEEERHKRHLLRSRLARLGFGTAAPGVWLAPAQLYEETRHTLERLQL
                     DPYVDLFTGAHAGFAPTADAVARWWDLDAIAAQHRAFLDEQEPVLQRWSRRRSVPPEAA
                     YRDYLLALDAWRHLPYADPGLPSPLLPEDWPGGRAAEVFGRLHTKLRAAGALYAHEAMT
                     GAQGLS"
     gene            complement(1134..1898)
                     /db_xref="GeneID:96282467"
                     /locus_tag="STRTU_RS07890"
                     /old_locus_tag="STRTU_001577"
     CDS             complement(1134..1898)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96282467"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602521.1"
                     /locus_tag="STRTU_RS07890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001577"
                     /product="HAD family hydrolase"
                     /protein_id="WP_159742892.1"
                     /sec_met_domain="HAD_2 (E-value: 6e-28, bitscore: 96.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVATEVGERPVRGVRGAGGETIKAAVFDLDGTLANTLPAISKLL
                     VKVASEQGCSVTARQAAAAIGKPPGPAFGRLLGKPEDDARVQAAISRYRELFAYEVLCL
                     GPQLLFPGVTAGLSALRAHGVELAVATSKTTRSAEALLDVMGIRDLVGPVVGQDMVRRG
                     KPHPEMVLRAAGRLGVAAWQSAYVGDTVGDMRMAVTARMEPVAVTYGVGTFGELAGVAG
                     TRMCGSFKDVVSVIAAARPRTGVKVGAQPRRR"
     misc_feature    complement(2062..2076)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.95"
                     /tool="antismash"
     misc_feature    complement(2088..2102)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            2349..2981
                     /db_xref="GeneID:96282468"
                     /locus_tag="STRTU_RS07895"
                     /old_locus_tag="STRTU_001578"
     CDS             2349..2981
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96282468"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088830.1"
                     /locus_tag="STRTU_RS07895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001578"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_159742893.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDAALEALQELEDSFTHSTEPLTTAVRDFTIELFGRVQSDAVLR
                     AGLRLRPETAPGLGIYALEQRWYALFLDKAVTCGTGGPAGPHASDTEPRRTAQLLTSIV
                     VGLLHLGGEDGTWWDHEAIVGLWALLPSAPGRIPAAVPASAQLPAAG"
     gene            complement(3012..4031)
                     /db_xref="GeneID:96282469"
                     /locus_tag="STRTU_RS07900"
                     /old_locus_tag="STRTU_001579"
     CDS             complement(3012..4031)
                     /NRPS_PKS="Domain: PKS_ER (92-337). E-value: 4e-22. Score:
                     70.7. Matches aSDomain:
                     nrpspksdomains_STRTU_RS07900_PKS_ER.1"
                     /codon_start=1
                     /db_xref="GeneID:96282469"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088829.1"
                     /locus_tag="STRTU_RS07900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001579"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_159742894.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 9.9e-19, bitscore:
                     66.3, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPTLVPAVHREVRLADQAEGELAARHFEIAEVPVPEPRTGQVLV
                     RNRIMAVTAAMRSQMTGIRLPMASFVRGEALWGAAVGEVVAAPGGGFTPGELVQHPYGW
                     REFAAVEESRVRRLAGDPLPTAAAYLSQGATAWGALRRAAGVRPGDTVFISGAAGGVGS
                     MAGQLARRLGAGRVVGSTGSERKAARLRAELGYDDTIVRGAGPIEQQLRRAAPDGIDVL
                     LDTVGGEQLTAALAVARPDARFALVGALSGQLGGERGAIAPVELDPGLIITRRVALRGF
                     GLYAHPDLPQEWTTEFGQGLRDGTLVFPHALLKGIEQAPQALRELTQGRHIGAVLVEL"
     aSDomain        complement(3021..3755)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRTU_RS07900_PKS_ER.1"
                     /evalue="4.00E-22"
                     /label="STRTU_RS07900_PKS_ER.1"
                     /locus_tag="STRTU_RS07900"
                     /protein_end="337"
                     /protein_start="92"
                     /score="70.7"
                     /tool="antismash"
                     /translation="TPGELVQHPYGWREFAAVEESRVRRLAGDPLPTAAAYLSQGATAW
                     GALRRAAGVRPGDTVFISGAAGGVGSMAGQLARRLGAGRVVGSTGSERKAARLRAELGY
                     DDTIVRGAGPIEQQLRRAAPDGIDVLLDTVGGEQLTAALAVARPDARFALVGALSGQLG
                     GERGAIAPVELDPGLIITRRVALRGFGLYAHPDLPQEWTTEFGQGLRDGTLVFPHALLK
                     GIEQAPQALRELTQGRHIGAVLV"
     CDS_motif       complement(3525..3587)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_STRTU_RS07900_0001"
                     /evalue="1.70E-04"
                     /label="PKSI-ER_m5"
                     /locus_tag="STRTU_RS07900"
                     /protein_end="169"
                     /protein_start="148"
                     /score="14.3"
                     /tool="antismash"
                     /translation="GDTVFISGAAGGVGSMAGQLA"
     misc_feature    4124..4142
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.84"
                     /tool="antismash"
     gene            4259..4855
                     /db_xref="GeneID:96282470"
                     /locus_tag="STRTU_RS07905"
                     /old_locus_tag="STRTU_001580"
     CDS             4259..4855
                     /codon_start=1
                     /db_xref="GeneID:96282470"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="STRTU_RS07905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001580"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_246240233.1"
                     /transl_table=11
                     /translation="MRTRRAVLEAAAHVIGTRGYQAATMAEIIQRAGVTKGAVYFHFTS
                     KDALARAVITEQTDPFLPQVSESRLQDAIDFTHQVALALRSDPLLQAGTRIAVETTFSE
                     DPLVPYQAWTDIITTMFTEARDNGELLPGVAPDRAAEFFVAAYMGVQLFSRAATNRADL
                     PERVTALWKHTLPGLASPGALSHLDPHGRCVQIPV"
     misc_feature    4646..4648
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4897..4910
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.34"
                     /tool="antismash"
     gene            5001..5978
                     /db_xref="GeneID:96282471"
                     /locus_tag="STRTU_RS07910"
                     /old_locus_tag="STRTU_001581"
     CDS             5001..5978
                     /codon_start=1
                     /db_xref="GeneID:96282471"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088827.1"
                     /locus_tag="STRTU_RS07910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001581"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_159742896.1"
                     /sec_met_domain="AfsA (E-value: 8.9e-21, bitscore: 72.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASATLTPSLRDQPHDTGARSSDGERPPALTTTVPREYVHRNALS
                     EVFLTDWRQDAADTWVVSAQWPRAHSFYGPLHGLHDPILLIETVRQSGILLSHVGHSVP
                     LDHPIIWQRVRYDLAPEALRTAQRPAEIELHVTDREMVHRGKRLVSALQEFRIVCDGAE
                     LASAMLDYSCHSPAVYRRLRGEYSDVALANSRAVPLPEAVSPALVAREHERDVVLSPTD
                     LPDRWQLRVDSSHPVLFDHPVDHAPGMLMVEAARQAAQAATPGCTLPVTLECSFSRYAE
                     LDAPTWVQARTTGQGDDDRRQVEITVEQHGQPVVSAQVTCVPVP"
     misc_feature    5028..5030
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    5283..5285
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6056..8383)
                     /db_xref="GeneID:96282472"
                     /locus_tag="STRTU_RS07915"
                     /old_locus_tag="STRTU_001582"
     CDS             complement(6056..8383)
                     /GO_function="GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96282472"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088826.1"
                     /locus_tag="STRTU_RS07915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001582"
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="WP_159746768.1"
                     /transl_table=11
                     /translation="MRVAVIGGGPGGLYAAVLLKRLDPTREITVWERNAPEDTFGFGVV
                     LSDETLGGIEHADPEVYRALQAEFVRWDDIDIVHRGRTLTSGGHGFAALGRRRLLAVLH
                     RRCHDLGIELRFRSEAPPAAELARSYDLVIAADGVHSATRTAHADAFRPQLTTHRCRYI
                     WLAADFAFDAFRFEIAETEHGVVQLHAYPFARAESGEHPGQPSAGTGGSAGASTVIVEM
                     REEVWRSAGLDRCDERESADWCAKVFADALGGRSLRGNNSSWTAFRTVVNDRWSHGNTV
                     LLGDAAHTAHFSIGSGTKLAVEDALALAACLQEQPDTARALAAYEEERRPVVASTQRAA
                     RASLAWFEELATYLDQPPHQFAFNLLTRSRRVTHDNLRLRDPGFVAAVEDEAGAPPGTP
                     PMFLPFRLGELTLRNRVVVSPMDMYSAADGNGTPGDFHLVHLGARALGGAGLVLTEMVC
                     VSPEGRITPGCTGLYAPEHETAWRRVTDFVHTSAPGTAIGVQLGHSGRKGSTRLMWDGI
                     DQPLPHGNWPLVAPSALPYRPGVNQTPHALAEPELALIRDQFVRSAQSAARAGFDLLEL
                     HCAHGYLLSGFLSPLTNHRTDAYGGDLTGRLRFPLEVFDAVRAVWPAGRPMTVRISATD
                     WADGGTTAADAVAMARAFAEHGADAIDVSTGQVVPDERPDFGRSYQTPFADRIRNTVGV
                     PVIAVGAISSWDDVNSLVLAGRTDLCALARPHLYDPHWTLHAAAEQGYAGPAAPWPLPY
                     QAGSRTPPTGRTDAPKPRLTLR"
     gene            complement(8386..9213)
                     /db_xref="GeneID:96282473"
                     /locus_tag="STRTU_RS07920"
                     /old_locus_tag="STRTU_001583"
     CDS             complement(8386..9213)
                     /NRPS_PKS="Domain: ECH (19-267). E-value: 2.3e-49. Score:
                     159.8. Matches aSDomain:
                     nrpspksdomains_STRTU_RS07920_ECH.1"
                     /codon_start=1
                     /db_xref="GeneID:96282473"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007445228.1"
                     /locus_tag="STRTU_RS07920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001583"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_159742897.1"
                     /transl_table=11
                     /translation="MSPFAGSARSTETWQHLRVTRQDGVATVTLARPDKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLAGEGRGFCSGGDVEEIIGATLGMDTAHLLDFNRMTGQVV
                     RAVRECPFPVIASVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRLLMLGDTVRAPEAERLGLISELADEGRADEAAAALARRLAEGPAMAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSDDYAEFHAAFTEKRAPKWQGK"
     aSDomain        complement(8413..9156)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_STRTU_RS07920_ECH.1"
                     /evalue="2.30E-49"
                     /label="STRTU_RS07920_ECH.1"
                     /locus_tag="STRTU_RS07920"
                     /protein_end="267"
                     /protein_start="19"
                     /score="159.8"
                     /tool="antismash"
                     /translation="TRQDGVATVTLARPDKLNALTFEAYADLRDLLAELSRERSVRALV
                     LAGEGRGFCSGGDVEEIIGATLGMDTAHLLDFNRMTGQVVRAVRECPFPVIASVHGVAA
                     GAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRLLMLGDT
                     VRAPEAERLGLISELADEGRADEAAAALARRLAEGPAMAHAQTKALLTAELDMPLAAAV
                     EMDAATQALLMNSDDYAEFHAAFTEK"
     misc_feature    complement(9293..9313)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 18.94"
                     /tool="antismash"
     gene            9659..10123
                     /db_xref="GeneID:96282474"
                     /locus_tag="STRTU_RS07925"
                     /old_locus_tag="STRTU_001584"
     CDS             9659..10123
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96282474"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /locus_tag="STRTU_RS07925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_001584"
                     /product="ATP-binding protein"
                     /protein_id="WP_246240236.1"
                     /transl_table=11
                     /translation="MQDLRMTADRTTAQLLTAELVTNVLKHTCGAAPIELVVEQRSAGC
                     RIEVHDDDPVLVEGLGDPLPLGDPGDGGDEDDRRAGPDPDAYADDSRATDDTAAADDTE
                     APAPDPACADRDGRGLVLIRSLSAASGCRPTPRGKAVWFTLPELRRPRRR"
ORIGIN
        1 gatgtgaccg aggcggcggt ggtcggccgg cccgacgagg accgcgggca ggtggtcgtc
       61 gcccatctcg tgctgcgggc cggggtcccc cgggacgacg acaccgtcgc cgcgctgcgc
      121 gccttcacca agacccggat cgcgccgtac aagtgcccgc gcgagatcgt cttccacacc
      181 tcgctgccgc gcaccccgac cggcaagctc cagcgcttcc ggctgcgggg acccgatcta
      241 gagtgacccg gtgaacgacc agcaaacgca gcgcacccca cggtccctga tcgtcacgtt
      301 ctacggcgcc tacgggcgcg gcgggcccgg tgctccggga ggcccggccg ctccggtgcc
      361 ggtggccgcc ctgatccggc tgctcggtgc gctcggggtc gatccgccgt cggtgcgctc
      421 cgcggtctcc cggctcaagc ggcgcgggct gctggccccc gcgcggacgg ccaccggcgc
      481 ggccgcgtac gggctgtccg acgcggcccg ccagctgctg gacgacggcg accgccggat
      541 ctacgcccgg cccgccgcgc ggctctccga cggctgggtg ctggccgtct tctcggtccc
      601 cgaggaggag cggcacaaac ggcatctgct gcgctcccgg ctggcccggc tcggtttcgg
      661 caccgccgcg ccgggtgtgt ggctcgcccc cgcccagctc tacgaggaga cccggcacac
      721 cctggagcgg ctgcaactgg acccgtacgt cgacctgttc accggtgcgc acgccggttt
      781 cgcccccacg gcggacgcgg tcgcccgctg gtgggacctg gacgcgatcg ccgcacagca
      841 ccgcgccttc ctcgacgagc aggagccggt gctccagcgc tggtcgcggc gccgctcggt
      901 gccccccgaa gcggcctacc gcgactatct gctggcgctg gacgcctggc gccacctgcc
      961 ctacgcggat cccggactgc cctctcccct cctgcctgag gactggccgg gcggccgcgc
     1021 tgccgaggtg ttcggccggc tgcacaccaa actccgggcc gccggggccc tgtacgcgca
     1081 cgaggcgatg accggggcgc agggcctgtc ttagcgaccc ccgcgcaccg gtctcagcgg
     1141 cggcgcggct gggcccccac cttgacgccg gtgcgcggcc gggcggcggc gatcacggag
     1201 accacgtcct tgaaggagcc gcacatccgg gttccagcga ccccggcgag ttcgccgaag
     1261 gtgccgacgc cgtacgtcac cgccaccggc tccatccgcg cggtcaccgc cattctcatg
     1321 tcccccacgg tgtcccccac atacgcgctc tgccaggccg cgacaccgag ccggccggcc
     1381 gcccgcagca ccatctccgg gtgcggtttg ccacgccgga ccatgtcctg gccgacgacc
     1441 gggccgacca ggtcgcggat gcccatgacg tcgagcagcg cttcggcgct gcgggtggtc
     1501 ttggacgtgg cgacggccag ctcgaccccg tgggcgcgca gcgcggacag ccccgcggtc
     1561 acgccgggga agagcagctg cggccccaga cacagcactt cgtaggcgaa cagctcgcgg
     1621 tagcgggaga tcgccgcctg cacccgggcg tcgtcctcgg gcttgcccag cagccggccg
     1681 aaggcggggc cgggaggctt gccgatggcg gcggccgcct gccgggcggt caccgaacag
     1741 ccctgctccg aggccacctt gaccagcagc ttgctgatcg cggggagggt gttggcgaga
     1801 gtgccgtcca gatcgaagac ggcggccttg atcgtctcac ctccggcgcc ccggacacct
     1861 ctcacgggac gttcgccgac ttccgttgct acgcgcatgg ctggagacct ttccggattg
     1921 cgatgggggg tgcaggggct cggatgacgt tgctggctcg gctgatgggg cggcctgcgg
     1981 tcgacgactg gaaacgggcg cggcggtctc gccatggcgc accgccgtcc ggggcggtac
     2041 gcaaccgtcg aggcggtgca tcggaagaga acgtcccgtt taccgccttc ggagatttaa
     2101 aaaccgcctg cgctgtttgt caatgcgtcc ccgtctcccc tgacgggtcg acaacacacc
     2161 tttgcccagc cagactggag gcagccgccc cccggcagag ccgagcgccc gcacgtgtga
     2221 ccgatcatca ccttcgcgtc agggcccgtg agttccggcc aatcctgggg aggatatcga
     2281 accgcatggt tggttttcta tgtagactgc aatgagctcg cagccgtact agagacggga
     2341 cggacaccgt gcccacccag catcgcgcca tccagagccg ccaggcactc atccgctcgg
     2401 ccgcagagac cttcctggag aagggggttc ccgcagcggg catggtcgag atcagccggc
     2461 gggcccggct gagcaaaggg gcgctctact tccacttcac ctccaaggac gacctcacgc
     2521 tcgcggtacg ggacgccgcg ctggaggccc tgcaggagct ggaggactcc ttcacccact
     2581 cgacggagcc actgaccacc gccgtacggg acttcaccat cgagctgttc ggccgggtgc
     2641 agtcggacgc cgtgctccgc gccggactgc ggctgcggcc ggagaccgcg ccggggctcg
     2701 gtatctacgc cctggagcag cgctggtatg cgctgtttct cgacaaagcg gtgacctgcg
     2761 ggaccggcgg accggccgga ccgcatgcct cggacaccga accccggcgc accgcccagc
     2821 tgttgacctc gatcgtggtg ggtctgctgc atctgggcgg cgaggacggg acgtggtggg
     2881 accacgaggc catcgtcggc ctgtgggcgc tgctgccgtc cgcaccgggc cggatcccgg
     2941 ccgccgtccc ggcctcggcg cagcttccgg cggccggctg atccggcgcc ccggtcctca
     3001 tgccgccggt ctcacaactc gaccagcacg gccccgatat ggcgcccctg cgtcagttca
     3061 cgcagcgcct gtggtgcctg ctcgatcccc ttcagcaggg catgcgggaa gaccagagtg
     3121 ccgtcccgca ggccctgacc gaactccgtg gtccactcct gcggcagatc cgggtgggcg
     3181 tacagcccga atccgcgcag tgccacccgc cgcgtgatga tcagccccgg gtccagctcc
     3241 accggggcga tggcgccgcg ttccccgccc aactggccgg agagtgcgcc gaccagcgcg
     3301 aaccgggcat cggggcgggc caccgcgagc gccgccgtca gctgttcgcc gcccacggtg
     3361 tccagcagga cgtcgatacc gtccggcgcg gcccgccgca gctgctgctc gatcggcccg
     3421 gcgccgcgca cgatcgtgtc gtcatacccc aactcggcgc gcagccgcgc ggctttgcgc
     3481 tccgagccgg tgctgccgac gacccgtccg gcgcccagcc gccgtgcgag ctgtccggcc
     3541 atactgccca cgccgcccgc cgcgccgctg atgaacacgg tgtccccggg cctgaccccg
     3601 gcggcccggc gcagggcgcc ccaggcggtc gcgccctgcg acagatacgc ggccgcggtc
     3661 ggcagcgggt ccccggcgag gcgccgtacg cgcgactcct cgacggcggc gaactcccgc
     3721 catccgtaag ggtgctggac cagttcgccg ggcgtgaagc cgcctccggg cgccgccaca
     3781 acctcgccga ccgcggcgcc ccacagcgcc tcaccccgca cgaatgaggc catcggcagc
     3841 cggattccgg tcatctgtga ccgcatcgcc gcggtcaccg ccataatccg gttgcgcacc
     3901 agcacctgac ccgtccgcgg ctcgggcacg ggcacctcgg cgatctcgaa atggcgtgcc
     3961 gccagttcgc cttcggcctg atcggcgagc cggacctcgc ggtggacggc ggggacgagg
     4021 gtggggggca tcggacagct cctcgctctg gtagcactgt ggcggcgccg cacgactggg
     4081 cgacgccggt tcacgcccga ggggcccgcc tcgggacagg cgcaaattca ataccgcatg
     4141 gttggcttct ttatgatgcg gaaatagact gcgcggccac gggcagcggg tcgcactcag
     4201 gcacaacaac tccaagagag catacggagg atgatcggtg gtcaagcagg aacgcgcagt
     4261 gcgcacccga cgcgccgtcc tcgaagcagc ggctcatgtc atcggcaccc gcgggtacca
     4321 ggccgccacg atggccgaga tcatccagcg cgccggggtc accaaagggg cggtgtactt
     4381 ccacttcacg tccaaggacg cactggcccg cgcggtgata acggagcaga ccgatccgtt
     4441 tctgccacag gtcagtgagt cccggctgca ggacgccatc gacttcaccc accaggtggc
     4501 gctggccctg cgcagcgacc cgttgctgca ggccggcacg cggatcgcgg tggagacgac
     4561 cttcagcgag gatccgctgg tgccgtacca ggcatggacc gacatcatca ccacgatgtt
     4621 caccgaggcc cgggacaacg gggaattact gcccggcgtg gcaccggaca gggcggccga
     4681 gttcttcgtc gccgcttaca tgggcgtgca gttgttctcg cgcgccgcga ccaatcgcgc
     4741 ggacctcccg gagcgggtca cggccctgtg gaagcacacg cttccgggtc tcgcgtcgcc
     4801 cggcgcgctg agccatctgg atccgcacgg ccgctgcgta cagatcccgg tctgacgcac
     4861 tgagtcaccg ccctgacgga gccgtcggct ccggtgactt ggaaaacaaa ccacatggtc
     4921 cgtatcttag agacgcagga acgctacgaa gcctcagccc gtcagggcgc tgagagccgc
     4981 acctcggaaa ggaacccccc atggccagcg ccacgctcac cccgtcctta cgcgatcagc
     5041 cgcacgacac cggagcccgg tcgtccgacg gagagcgacc gccggccctc accacgaccg
     5101 tgccgcgcga gtatgtccac cgcaacgcgc tgtcagaggt gttcctgacg gactggcggc
     5161 aggacgcggc cgacacctgg gtggtcagcg cccagtggcc gcgcgcccac agcttctacg
     5221 ggccgctcca cgggctgcac gacccgatcc tgctgataga gaccgtgcgg cagtccggca
     5281 tattactgag ccatgtcggg cacagcgtgc ccctcgacca cccgatcatc tggcagcggg
     5341 tccgctacga cctcgcgccg gaggcgctgc gcaccgcaca gcggcccgcc gagatcgaac
     5401 tgcacgtcac cgaccgcgag atggtccacc gcggcaagcg tctggtcagc gccctccagg
     5461 agttccggat cgtgtgcgac ggcgccgaac tggcctccgc gatgctcgac tactcctgtc
     5521 acagcccggc cgtctaccgc cggctgcgcg gcgagtacag cgatgtggcg ctcgccaact
     5581 cccgggcggt gccgctgccc gaggccgtga gtcccgcgct ggtcgcccgg gaacacgaac
     5641 gcgatgtggt gctctccccc accgaccttc ccgaccgctg gcagctgcgg gtggacagct
     5701 cgcacccggt cctcttcgac caccccgtcg accacgcccc cggcatgctg atggtcgagg
     5761 ccgcccggca ggccgcccag gccgccacac ccggctgcac gctgccggtc accctggagt
     5821 gctccttctc ccggtacgcc gaactcgacg caccgacctg ggttcaggcg cggaccaccg
     5881 gccagggcga cgacgaccgc cgacaggtcg agatcaccgt cgaacagcac ggccagccgg
     5941 tcgtctcggc gcaggtcacc tgcgtgccgg tgccctgatc agccgccgcc ccggcccgcc
     6001 gacgccctga ccgggccccg gaaacggccc cgccccgctg cccgtgcctc ccccgtcacc
     6061 gcagcgtgag gcggggcttc ggcgcgtccg tgcgcccggt gggcggggtg cggctgcccg
     6121 cctggtacgg cagtggccag ggggcggccg gccccgcgta cccctgctcc gcggcggcgt
     6181 gcagcgtcca gtgcgggtcg tacagatgcg gtcgggccag cgcgcacagg tccgtccgcc
     6241 cggccagcac cagggagttg acgtcgtccc aggaggagat ggcgccgacc gcgatgaccg
     6301 ggacgccgac ggtgttgcgg atgcggtcgg cgaacggggt ctggtaggag cggccgaagt
     6361 cggggcgctc gtcgggcacc acctgaccgg tggacacatc gatggcgtcg gcgccgtgct
     6421 cggcgaaggc ccgcgccatc gccaccgcgt ccgccgcggt ggtaccgccg tccgcccagt
     6481 ccgtcgcgga gatacggacg gtcatcgggc gcccggccgg ccagacggcg cggaccgcgt
     6541 cgaagacttc cagcgggaag cgcagccggc cggtgaggtc gccgccgtag gcgtcggtgc
     6601 ggtggttggt gagcggcgag aggaagccgg aaagcagata gccgtgggcg cagtggagtt
     6661 cgagcagatc gaaaccggcg cgggcggcgg attgcgcgga ccggacgaac tggtcgcgga
     6721 tgagggcgag ttcgggctcg gcgagggcgt gcggggtctg gttgacgccg gggcggtagg
     6781 gcagtgcgga gggcgccacg aggggccagt tgccgtgcgg gagcggctgg tcgatgccgt
     6841 cccacatcag ccgcgtcgag cccttgcggc cggagtggcc gagctggacg ccgatggcgg
     6901 tgccgggggc ggaggtgtgc acgaagtcgg tgacccggcg ccaggccgtc tcgtgctccg
     6961 gcgcgtacag ccccgtacag ccgggcgtga tccgcccctc ggggctgacg cacaccatct
     7021 ccgtcagcac cagcccggcg ccgcccagcg cgcgggcgcc gaggtggacg aggtggaagt
     7081 cgccgggggt gccgttgccg tccgcggcgg agtacatgtc catcggggag accacgacgc
     7141 ggttgcgcag cgtcagctca cccagtcgga acggcaggaa catcgggggt gtgccgggcg
     7201 gggcgcccgc ctcgtcctcc acggccgcga cgaagccggg gtcgcgcagc cgcagattgt
     7261 catgggtgac ccggcggctg cgggtgagga ggttgaacgc gaactggtgc ggcggctggt
     7321 cgagataggt ggccagctcc tcgaaccagg cgaggctggc gcgggcggcg cgctgggtgg
     7381 aggcgacgac ggggcggcgc tcctcctcgt acgcggccag cgcacgggcc gtgtcgggct
     7441 gctcctgaag gcaggccgcc agcgccaggg cgtcttcgac ggccagcttg gtgccggagc
     7501 cgatggagaa gtgcgcggtg tgtgcggcat cgccgagcag cacggtgttg ccgtgcgacc
     7561 agcggtcgtt gacgaccgtg cggaaggccg tccaactgga gttgttgccg cgcagcgacc
     7621 ggccgccgag ggcgtcggcg aagaccttgg cgcaccagtc ggccgactcg cgctcatcgc
     7681 agcggtcgag tcctgccgac cgccagactt cctcgcgcat ctcgacgatg accgtactgg
     7741 caccggcgga gcccccggtt ccggcggacg gttgcccggg gtgctcgccg ctctccgcac
     7801 gggcgaaggg gtaggcgtgg agctgtacga cgccgtgctc ggtctccgcg atctcgaagc
     7861 ggaaggcgtc gaaggcgaag tcggcggcga gccagatgta gcggcagcgg tgggtggtca
     7921 gctgcgggcg gaaggcgtcg gcgtgtgcgg tacgggtcgc gctgtgcacc ccgtcggcgg
     7981 cgatcaccag gtcgtaggag cgggccagtt cggcggcggg cggtgcctcg gagcggaagc
     8041 gcagctcgat gccgaggtcg tggcagcgcc ggtggagtac ggccagcagc cggcgccgcc
     8101 cgagcgccgc gaagccgtgc ccgccggagg tcagggtgcg gccccggtgg acgatgtcga
     8161 tatcgtccca gcggacgaac tcggcctgga gggcgcggta gacctccggg tcggcgtgct
     8221 cgatgccgcc gagggtctca tcggagagca cgacgccgaa gccgaaggtg tcctcgggcg
     8281 cgttgcgctc ccatacggtg atctcacggg tggggtcgag gcgcttgagc aggaccgcgg
     8341 cgtacagccc gcccggcccg ccgccgatga cggcgacccg catggctatt tcccctgcca
     8401 cttgggggcc cgcttctcgg tgaaggcggc gtggaattcc gcgtagtcgt cgctgttcat
     8461 cagcagcgcc tgggtggcgg cgtccatctc cacggcggcg gccagcggca tatcgagctc
     8521 ggcggtgagc agcgccttgg tctgggcgtg ggccatcgcg ggcccctcgg cgagccgccg
     8581 ggcgagcgcc gcggccgcct cgtcggcccg gccctcatcg gccaactcgc tgatcaggcc
     8641 gagccgttcg gcctccggtg cgcggacggt gtcgccgagc atcagcagcc gggtggcgtg
     8701 gccgaggccg atgacgcggg gcagcagata ggcggcgccc atgtcgccgc cggagaggcc
     8761 gacccgggtg aagaggaagg cgaaccgggc ggacgggtcg gcgacacgga agtccgcggc
     8821 cagtgcgagc accgcaccgg cgcccgccgc gaccccgtgc accgacgcga tcaccgggaa
     8881 cgggcactcc cgcaccgccc ggacgacctg cccggtcatc cggttgaagt ccagcaagtg
     8941 cgcggtgtcc atgcccaggg tggcgccgat gatctcctcg acatcgccgc cggagcagaa
     9001 tccgcgcccc tcccccgcga gcaccagcgc gcgtacggaa cgctccctgg acagctcggc
     9061 gagcaggtcg cggaggtcgg cgtaggcctc gaaggtgagc gcgttgagct tgtccgggcg
     9121 ggccagggtg acggtggcga ccccgtcctg ccgcgtcaca cggagatgtt gccaggtctc
     9181 ggtactgcgg gcggatcctg cgaacgggct catcgggctg cctgccccct ttggccggtc
     9241 gggccggtgg atgctgttcc ggatgctctc cgaagttatc actccttcat gactgtcgtc
     9301 atgagaacgc gatacgacac ggggcggaag acggcctctc gcccactcgc cgggaccgcc
     9361 gggactgccg ggaccgtcgg gaccgccagg accgccagga ccgtcaggtt gaccggccga
     9421 aggtcccggc cgccacatgc cgtaggtccg tgccatcccc ggccgcccgc ccctgatccc
     9481 ccgcctccgc gctttctacc gtggaggcat cccgatcccg cctcaggccc gccggacagg
     9541 gcttctgcga acggacgaac cccgcatgct ggaaccgccg ccccctgcga cctggcgcat
     9601 cacgctgccg cgcggtgcga ccgcggtccc catcgcccgt gcgatggtaa ggaccgccat
     9661 gcaggatctc cggatgacgg ccgaccggac cacggcccag ctgctgaccg ccgagctggt
     9721 caccaacgtg ctcaagcaca cctgcggcgc ggcccccatc gagctggtgg tggagcagcg
     9781 ctcggcgggc tgccggatcg aggtgcacga cgacgatccg gtgctcgtgg aggggctcgg
     9841 cgatccgctg ccgctggggg atccgggtga cggcggcgac gaggacgacc ggcgggcggg
     9901 gcccgacccc gatgcctacg ccgacgactc ccgcgccacg gacgacaccg ccgccgcgga
     9961 cgacaccgag gcccccgccc ccgaccccgc ctgcgccgac cgcgacggcc gtggtctggt
    10021 gctgatccgt tccctcagcg ccgcctccgg ctgccgtccg acgccgcgcg gcaaggcggt
    10081 ctggttcacc cttcccgagc tgcggcgccc gcgccggcgc tgacgggggc cgcaccggcc
    10141 gtgcccgcgt cggccgccgg gctctgctgc gccgcagcga gccgcgagcg gcggcgcccg
    10201 tagcacgcgt agacgagcgc gcccgccacc aggaacccgg cgaactggag ccaggtcgcc
    10261 ggaccggtgc cgtacatcag atagaggcag aaggcgatgc cgagcaccgg gctgaccggg
    10321 aagagcggga cgcggaagcg gcgcggcaga tcggggcggg tgcggcggag caccatcaca
    10381 ctgaggttga ccaccgccat gaccgccagc gtcccgatgg tcgacaggtt catcaccgcg
    10441 tccaacggga cgaccgcggc cggcacggcg aagacggccg tgacgatcca ggtgttggcg
    10501 gcgggggtgg acgtccgggg tgagacccgc tcgaagatcc gcgggaccag tccgtcccgc
    10561 gacatcgaca tcaggatgcg ggtctggccg tacagcaccg cgaggacgac cgaggcgatg
    10621 gcgactaccg caccgaacgc gatcaccccg ccgccgagcg ccgagtcggt gacgtggttg
    10681 acgatcagcg acagcgcggc gggccgtccg gcgacctggt cggcggagag cgcgccgatg
    10741 gcggcgaccg cgaccaggca gtacaggacg gtgaccacac cgagggacac catgatcgcc
    10801 agcgggatgt tccgccgcgg gtccctgacc tcctcaccgg ccgtggtgat cgcgtcgaag
    10861 ccgatgtacg agaagaaggc gagcgaggcg ccggaggtga tgccggcgac gccctccggg
    10921 gcgaacggcg tcagattgcc ggcccggaag gcgctgaggc cgatcacaca gaacagga
//
