LOCUS       NZ_CP114205            10984 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces tubercidicus strain DSM 40261 chromosome, complete
            genome.
ACCESSION   NZ_CP114205
VERSION     NZ_CP114205.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces tubercidicus
  ORGANISM  Streptomyces tubercidicus
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8284047)
  AUTHORS   Ruckert,C., Busche,T., Kalinowski,J. and Wittmann,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-MAR-2022
            Assembly Method                   :: flye v. 2.9; Newbler v. 2.8
            Assembly Name                     :: DSM40261_v1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 133.7x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:53:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,167
            CDSs (total)                      :: 7,077
            Genes (coding)                    :: 6,906
            CDSs (with protein)               :: 6,906
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 72 of 171
            Pseudo Genes (incomplete)         :: 133 of 171
            Pseudo Genes (internal stop)      :: 9 of 171
            Pseudo Genes (multiple problems)  :: 41 of 171
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:04:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 390650
            Orig. end                         :: 401634
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114205.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from DSM 40261.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="5"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5984
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(73..88)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.2"
                     /tool="antismash"
     misc_feature    complement(606..623)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.36"
                     /tool="antismash"
     misc_feature    complement(617..634)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    complement(863..878)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.88"
                     /tool="antismash"
     gene            892..1425
                     /db_xref="GeneID:96281242"
                     /locus_tag="STRTU_RS01710"
                     /old_locus_tag="STRTU_000342"
     CDS             892..1425
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96281242"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092048.1"
                     /locus_tag="STRTU_RS01710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000342"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_159741878.1"
                     /transl_table=11
                     /translation="MTEIRTPRLLLRRWTDDDLAPMADINADPQVMRWIGDGSTLDLDR
                     TAEEIEGWEEEWDDEGFGLFAVELLASGELAGCAGLSVPAFLPEARHEVAISWRLGRQY
                     WGQGYASEAAQAVLEFALQDRGLDRVIALNPLGHESSLNVVRKLGMAFEREAAHPEYGH
                     ALTVHSIDLTEYQG"
     gene            complement(1477..3747)
                     /db_xref="GeneID:96281243"
                     /locus_tag="STRTU_RS01715"
                     /old_locus_tag="STRTU_000343"
     CDS             complement(1477..3747)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96281243"
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537753.1"
                     /locus_tag="STRTU_RS01715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000343"
                     /product="MFS transporter"
                     /protein_id="WP_269777322.1"
                     /transl_table=11
                     /translation="MTRTTTDPLPARAADTTATPTAPDPAAGGPHRAPRGIVPVLAFSG
                     IVVAVMQTLLVPVIKDLPVLLNTAPSNATWVMTATLLAGAVSTPIMGRLGDLYGKRRML
                     LTSLAVMVVGSLICGFTSDLVTMIAGRALQGFAMGAIPLGIGIMRDELPRERLGSAMGL
                     MSSSIGVGGGLALPVAALVAQHADWHALFFGAAGLGVLSMLLTLLVVPETAIRAQGRFD
                     VAGALGLSAGLVALLLPITKGSDWGWGAPVTLVLFGVAALILVLWGVMELRRADPLVDL
                     RTTARREVLLTNLASITVGVAFYAISLVLPQLLQLPTSTGYGLGQSMVVAGLCMAPLGL
                     TMMLVAPLYARIAARRGPKVSLLLGMLVIAIGYGAGIGLMSAAWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDASETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSQSVGPVTVPTM
                     AGFRVSFLIATAAVLLGVALACFLPSERKAPRPTLVAQSTEDPADAGFRGRVANTSGGP
                     VPGASITLIDRQGRQAGITTAGPDGHFALGAPATGTYLLTAAAPGHTPQATSATYRAEG
                     ASTSVDLVLASTGRLGGTLIGGTEGDPLADGSIVVTDAEGAVVARTTSGADGRWDAPHL
                     PPGQYTLVLSAAGHQPQARTVELSGGAPERQDARLKPTATVRGTVRGPNGRPLADAAVS
                     LVEDGTVAGHTVTGPDGGFAFSGLSGGPHYTLTASGYPPHAAPLSLTPGASKTLDLELD
                     LAP"
     gene            complement(4140..4826)
                     /db_xref="GeneID:96281244"
                     /locus_tag="STRTU_RS01720"
                     /old_locus_tag="STRTU_000344"
     CDS             complement(4140..4826)
                     /codon_start=1
                     /db_xref="GeneID:96281244"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="STRTU_RS01720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000344"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_159741879.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRLILEAAASVFDEQGYDRATIAEVLERAGMTKGA
                     LYFHFASKEQLALAVLEEQVLDIAVEPQKIKLQEFVDSGQVLAFRLRSDPIQRGAARLA
                     VEQGSNHLDRKQSMLSWTRFVVGMLNEARDRGEILDSIVVQESAELFVGAFAGLQMMSQ
                     ALTNRADLNRRLTIFFQHTLPSIAVPAILARLNLDAERGAQLDAALQGKALANAEADAD
                     AGVVAD"
     misc_feature    4926..4939
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            5001..5984
                     /db_xref="GeneID:96281245"
                     /locus_tag="STRTU_RS01725"
                     /old_locus_tag="STRTU_000345"
     CDS             5001..5984
                     /codon_start=1
                     /db_xref="GeneID:96281245"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="STRTU_RS01725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000345"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_159741880.1"
                     /sec_met_domain="AfsA (E-value: 7.1e-19, bitscore: 66.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTMHVNGTVPPRSPHRPHTGGDRAATPQSRHGIQVTRELVHRP
                     FADDILVTSWHRLDDAHFSLTAQWPHDHGYFTPHQGRHHLILTGETIRQAGLLLCHAEL
                     GVPLGHHFILGDLVYTTHPEHLAVGSGPTLLTIDVVCSRMRMRAGTLARGRFDMTIRKA
                     GRIVATGHSDVTVTGPRGYQLLRGDRLTARHTPGALPAPVTPRLTGSTKCRDVLLSPAG
                     RPDRWLLRIPPGHPAVVNPANDHIPGMVLLDAAQQAARVLTAPDAFVPYAFGTDFRRYA
                     EHGTPCVIEARRVPSALPSTTTVQVTGLQGGDPVFVSTLTALDPRG"
     gene            complement(6040..6831)
                     /db_xref="GeneID:96281246"
                     /locus_tag="STRTU_RS01730"
                     /old_locus_tag="STRTU_000346"
     CDS             complement(6040..6831)
                     /codon_start=1
                     /db_xref="GeneID:96281246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092053.1"
                     /locus_tag="STRTU_RS01730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000346"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_159741881.1"
                     /transl_table=11
                     /translation="MTTTTTTATRTTERHSRPRTLAQRVAAADWPALAEELDTLGCAVT
                     PRLLSPADCRALSALYDEPAEAQGTESGGRSRFRSTIDMARHRFGSGQYRYFANPLPEP
                     VQALREAFYPHLLKIARDWADKLGRPAPWPDSLEEWLTPCHEAGQTKSSQILLRYGAGD
                     WNALHRDLFGEMVFPLQVVIGLDEQGIDYTGGEFLLVEQRLRAQSRGTSTVLPQGHGLI
                     FTTRDRPVRSARGWSAGPVRHGVSAVRSGRRHSLGLVFHDA"
     gene            complement(6893..7405)
                     /db_xref="GeneID:96281247"
                     /locus_tag="STRTU_RS01735"
                     /old_locus_tag="STRTU_000347"
     CDS             complement(6893..7405)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96281247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="STRTU_RS01735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000347"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_159741882.1"
                     /transl_table=11
                     /translation="MTLYTTLDSPLGELLLVGEESATAPGGIALASLSVPGQKGGATVQ
                     DGWTYAPTAFDGIADQLRAYFDGELTRFAIACAPTRGTDFQRRVWQALEAIPYGTTTTY
                     GKVAADIGAARGAVRAIGTAIGANPLLVIRPCHRVIAADGSLSGYAGGPVRKRQLLGIE
                     GALPEAH"
     gene            complement(7402..8922)
                     /db_xref="GeneID:96281248"
                     /locus_tag="STRTU_RS01740"
                     /old_locus_tag="STRTU_000348"
     CDS             complement(7402..8922)
                     /codon_start=1
                     /db_xref="GeneID:96281248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092055.1"
                     /locus_tag="STRTU_RS01740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000348"
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="WP_159741883.1"
                     /transl_table=11
                     /translation="MNEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VAFFRTAAAAQGSGFRACRRCRPDAVPGSPEWNARADVVGRAMRLISEGVVDREGVSGL
                     AARLGYSARQVQRQLTAELGAGPVALARAQRAHTARVLLQTTGLQAAEIAFAAGFASVR
                     QFNDTIKEIYALTPSELRAARPGKATRFGPVASPTTPGVLPLRLAFRGPFAARELFDHL
                     QRRAITGIEEVSGEPGARTYRRTLRLPHGAGIAEVDEAAGDGWLDCRLHLAELRDLTTA
                     SQRMRRLFDLDADPYAVAERLGADAVLARLVDRRPGLRSPGAPAPDELAVRAVLGQQVS
                     VAAGRTLGGALVAAYGEPLPEPAGALTHLFPRIDDLAQAALTELGMPESRRATLRTLSN
                     ALADGTVVLDAGADRDEAERALLGLRGIGPWTAGYIRMRALGDPDVLLTGDVAVLAGMR
                     RAGAPAAGLRELAETWRPWRSYAMHHFWNVPLTEPANPCPTTTESTGP"
     gene            complement(9078..9245)
                     /db_xref="GeneID:96281249"
                     /locus_tag="STRTU_RS01745"
                     /old_locus_tag="STRTU_000349"
     CDS             complement(9078..9245)
                     /codon_start=1
                     /db_xref="GeneID:96281249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608013.1"
                     /locus_tag="STRTU_RS01745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000349"
                     /product="hypothetical protein"
                     /protein_id="WP_167539106.1"
                     /transl_table=11
                     /translation="MSDDSGNSDSSDDGVFSTRGSGSGGSSDLSVLRRAMRWETLVILL
                     ILVILTVVAS"
     misc_feature    9210..9224
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 18.38"
                     /tool="antismash"
     gene            complement(9378..9605)
                     /db_xref="GeneID:96281250"
                     /locus_tag="STRTU_RS01750"
                     /old_locus_tag="STRTU_000350"
     CDS             complement(9378..9605)
                     /codon_start=1
                     /db_xref="GeneID:96281250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="STRTU_RS01750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000350"
                     /product="hypothetical protein"
                     /protein_id="WP_159741884.1"
                     /transl_table=11
                     /translation="MSEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     TGPAGSIALDDPRLVEWRGGSLDDWPMPPA"
     gene            9697..10302
                     /db_xref="GeneID:96281251"
                     /locus_tag="STRTU_RS01755"
                     /old_locus_tag="STRTU_000351"
     CDS             9697..10302
                     /codon_start=1
                     /db_xref="GeneID:96281251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971478.1"
                     /locus_tag="STRTU_RS01755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="STRTU_000351"
                     /product="HD domain-containing protein"
                     /protein_id="WP_159741885.1"
                     /transl_table=11
                     /translation="MTMDDLVIPDTRACRTALEVASAYCSPALLNHSVRAYLWAAGYAS
                     ANGIAFDPELLYVSAMFHDIGLVKEFDSHTVPFEEAGGHVAWVFGAGAGWPVERRVRAS
                     EVIVRHMWDEVDAAMDPEAHLLVYSTSLDISGRRPDTLPDDLRTEVLARYPRLGLGEEF
                     LACFRDQAGRKPDSTAAAAVREGIAARISANPLDAYGT"
     misc_feature    complement(10480..10495)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     misc_feature    complement(10548..10563)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.75"
                     /tool="antismash"
     misc_feature    10905..10920
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.52"
                     /tool="antismash"
ORIGIN
        1 ccccgcgggt ggcgggcttg ccgacgggag agccgtcccg gttgatcagc gagacccccg
       61 ggaagccacg cagcgaacag gccttcttgc ccttgttcgt gaagacgagg gggtagcgga
      121 tgttgcccgc acccgcgtcg gcggcgccca ggcgcagcga catattggcg gcggtgcagc
      181 ggtccgaggt cgcctctgcg gaggtgtggg cctccgcggc gctcggggcc ttgcccgtcg
      241 gcttgccccc gggcggggcc gccgacttcc ccgacggacg ctgcccgccg gtgccggact
      301 gttccttcgc accggacgcc tgtgcactgc cctcggacga cggggagccg ccggttgcct
      361 tgtcgtggga cgtggcggag gcggccgacg aggggcggga ggcggcgtcc gggccgccct
      421 cacaggcggt gagcgcaagc gccgcgacag cgatcagcga gccggcggcg acacgacgac
      481 cgcggccgag cgacttggtg gtgagcgaca taagtgtccc cctcgtctgg taggagcgag
      541 ctctcgtagt ggggtgcggt ccgtccgttg ctcttctcag cgtggccctg ggcactgccg
      601 gtccgctaac gaggcactaa cacaccacta acgagcctgt gtgctcgaca cgccgaaaac
      661 ccctgcaacc gctgtgaccg gccgggattc ggagtgccgc tcagactcgc agaaaactcc
      721 cgacttgcgg acccggaacc gccgggttca gccgttccgc cgacaagatc accgggccgc
      781 accggtcacg gcgggccccg gcggtccccg caggcccacc cgccccggcg gcccctgcac
      841 ggacacccgc cgattaccgc ccgcgaccgc gcattcgaga ggatgggcgg catgaccgag
      901 atccgcaccc cccgcctcct cctccgccgc tggaccgacg acgacctcgc ccccatggcg
      961 gacatcaacg cggacccgca ggtcatgcgc tggatcggcg atggctcgac gctcgacctg
     1021 gaccgtaccg ccgaggaaat cgagggttgg gaggaggagt gggacgacga gggcttcggc
     1081 ctcttcgccg tcgaactgct cgcctcgggc gaactggccg gatgcgccgg cctgtccgta
     1141 cccgcgttcc tgccggaggc ccggcacgag gtggcgatca gctggcggct gggccggcag
     1201 tactggggac agggctacgc ctccgaggcc gcgcaggccg tgctggagtt cgccctccag
     1261 gaccgcggcc tggaccgtgt catcgctctc aacccgctcg gccatgagtc ctccctgaac
     1321 gtcgtacgca agctcggcat ggccttcgag cgggaggcgg cacatccgga gtacggccat
     1381 gcgctgacgg tgcacagcat cgatctcacg gaataccagg gctgagcggc acgcgggcgg
     1441 ggcccggcgg gcgccgcggg gtgggtgtgt gccggcctat ggggccaggt ccagctccag
     1501 gtccagggtt ttgctggcgc cgggggtcag ggataggggg gcggcgtgtg gcgggtagcc
     1561 ggacgcggtg agggtgtagt gggggccgcc gctcaggccg gagaaggcga agccgccgtc
     1621 cgggccggtg acggtgtgtc cggcgaccgt gccgtcctcg acgaggctca cggcggcgtc
     1681 ggcgagcggc cggccgttcg ggccccggac cgtgccgcgg acggtggccg tcggcttcag
     1741 gcgggcgtcc tgccgttccg gagcgccgcc ggagagttcc acggtgcggg cctgcggctg
     1801 gtgaccggcg gcgctgagga ccagggtgta ctggccgggc ggcaggtgcg gggcgtccca
     1861 gcgcccgtcc gcaccggatg tcgtacgggc caccacggcg ccctcggcat cggtgacgac
     1921 gatgctcccg tcggccagcg ggtcgccttc ggtcccgcct atcagagtgc cgcccagtcg
     1981 gccggtactg gcgaggacga ggtcgacgct ggtcgaagca ccctcggcgc gataggtggc
     2041 ggaggtcgcc tgcggagtgt ggccgggcgc ggcagccgtg aggaggtagg tgccggtcgc
     2101 gggggcgccg agcgcgaagt gaccgtcggg accggccgtg gtgattccgg cctggcggcc
     2161 ctgccggtct atcagggtga tgctcgcacc ggggaccggg ccgccggagg tgttcgccac
     2221 ccggccccgg aagcctgcgt ccgccgggtc ctcggtgctc tgggcgacca gggtcggtcg
     2281 gggcgccttc cgctcggacg gcaggaagca ggccagggcg acgccgagga ggaccgcggc
     2341 cgtggcgatg aggaaggaga cccggaagcc ggccatcgtg ggcaccgtga ccggtccgac
     2401 gctctgggac atatgggcca gcaccatgcc gatcaccgcg ctggagaccg aggtgccgat
     2461 ggagcgcatc agggtgttca ggccgttggc cgcaccggtc tccgaggcgt cgacggcacc
     2521 gatgatcagt gcgggcagcg aggagtacgc caggccgata ccggcgccga cgaccaccgc
     2581 gatgatgacg gtctgccagg cggcactcat caggccgatg cccgcgccat agccgatcgc
     2641 gatgaccagc atgccgagca gcagcgagac cttggggccg cgccgggcgg cgatccgtgc
     2701 gtacagcggg gcgacgagca tcatcgtcag gcccagtggc gccatgcaca gcccggccac
     2761 caccatcgac tggcccaggc cgtaaccggt cgacgtgggc agttgcagca gctgcggcag
     2821 caccagggag atcgcgtaga acgccacacc gaccgtgatg gaggcgaggt tggtcagcag
     2881 cacctcacgg cgcgcggtgg tccgcagatc gaccagcgga tcggcacgac gcagctccat
     2941 cacgccccac agcaccagga tcagggccgc gacaccgaag aggacgaggg tgaccggcgc
     3001 accccagccc cagtcgctgc ccttggtgat gggcagcagc agggccacca gaccggccga
     3061 cagccccagg gcgcccgcca cgtcgaagcg cccctgggcc cgtatcgcgg tctccggtac
     3121 gacgaggagg gtgagcagca tcgacagcac gcccagcccg gcggcgccga agaacagggc
     3181 gtgccagtcg gcgtgttggg ccaccagggc cgcgaccggc agcgcgagcc cgccgccgac
     3241 gccgatcgat gagctcatca ggcccatggc cgagccgagc cgctcgcgcg gcagctcgtc
     3301 gcgcatgatg ccgatgccga ggggaatggc acccatcgcg aagccctgca gcgcccggcc
     3361 cgcgatcatg gtgaccaggt cactggtgaa gccgcagatc aacgagccga cgaccatgac
     3421 cgcgaggctg gtcagcagca tccgccgctt accgtagaga tctccgaggc gccccatgat
     3481 cggggtcgag acggcgccgg cgagaagggt ggcggtcatg acccaggtgg cattgctggg
     3541 tgccgtgttg agcagcaccg gcaggtcctt gatgaccggc acgagcaggg tctgcatcac
     3601 cgcgacgacg atgccggaga aggcgaggac cgggacgatg ccgcgggggg ctctgtgcgg
     3661 cccgccagcg gccgggtcgg gtgccgtggg cgtggccgtg gtgtccgccg cccttgcggg
     3721 gagtgggtcc gtcgtcgtcc gggtcatgcg tgcggcctcc agggcggcag aggtgagtgg
     3781 gtgccgtcag cagatgacgg agcggagtgc gagagcggta cgggccggct cgggccttcc
     3841 gagtccgccg aattcagtga actacacgcc actgacaatc ccccgcgagc agcgttcgtc
     3901 gtgcacctac cagcccccgt tcacggtccg cggcgtcctg ctgtcatctc aacagcgaga
     3961 ggcgccgcgg ctcttccgcg acgcctctga cctgcacaac tcatccacga ggtgcacaca
     4021 cttccgcgtc cgacaccgcg cacgcggtat gttttcagcg accttgcggc gaaatgctct
     4081 ccgccctccg cgccccggca gccgcacacc ggccggggcg ggctcagtcc ggggcgggct
     4141 cagtccgcga ccactccggc atcagcatct gcctcggcgt tcgccaacgc ctttccctgc
     4201 aaggcggcat cgagttgcgc gccccgctcc gcgtccagat tgagcctggc gaggatggcc
     4261 ggcacggcga tgctggggag ggtgtgctgg aagaaaatcg tcagcctgcg gttgaggtcg
     4321 gcccggttgg tcagggcctg cgacatcatc tgcagcccgg cgaaggcacc gacgaacaac
     4381 tcggcggact cctgcaccac aatgctgtcc aggatctccc cgcggtcccg ggcctcgttc
     4441 aacatgccca cgacgaaccg ggtccacgag agcatcgact gcttgcggtc gagatggttg
     4501 gagccctgct cgaccgccag ccgcgccgca ccccgctgga tcgggtcgct gcgtagccgg
     4561 aaggccagta cctggcccga gtcgacgaat tcctgcagct tgatcttctg cggctccacg
     4621 gcgatatcga gtacctgctc ctccagcacc gcgagcgcca actgctcctt ggaggcgaag
     4681 tggaagtaca gggcgccctt cgtcatgccg gcgcgctcca ggacttcggc gatcgtggcg
     4741 cggtcgtagc cctgctcgtc gaaaaccgac gcggccgcct ccaagatcaa tcgccgggtc
     4801 cgaatcgcgc ggtcctgttg tgccacagga cgcctcctgt ctgtcgatgt gtccacgttc
     4861 cgcaacagtt agctacagcc caagtcgccg ttggatcagc tactttccct ttttcgcccg
     4921 cccggcattg aaaagaaaac cggatggtac gtatcttatc aacgatgtac ctcgatccgt
     4981 agcgatgcct gggggcagtc atgagtgaca cgatgcacgt caacggcacc gttccaccca
     5041 ggtctccgca ccgtccgcac accggcgggg accgggccgc caccccgcag agccgccacg
     5101 gcatccaggt cacccgggag ttggtacacc gccccttcgc ggacgacatt ctggtcacct
     5161 catggcaccg gctggatgac gcgcacttct cgttgacggc ccagtggccg cacgatcacg
     5221 ggtacttcac cccccatcaa ggccgacacc acctcatcct cacgggtgag accatccgcc
     5281 aggccgggtt gctgctctgc cacgccgaac tcggcgtacc gctcggccac cacttcatcc
     5341 tcggcgacct tgtgtacacc actcaccccg agcatctcgc ggtgggcagc gggcccacgc
     5401 tgctgaccat cgacgtcgtc tgcagccgga tgcgcatgcg ggccggcacg ctggcccgtg
     5461 gccgcttcga catgaccatc cgcaaggcgg gccggatcgt cgcgaccggc cactcggatg
     5521 tcacggtcac cggaccccgt ggctaccagc tgctccgcgg cgatcgactg accgcgcggc
     5581 acaccccggg cgcccttccc gcccccgtta cgccccgact gaccggcagc accaagtgcc
     5641 gcgatgttct gctctccccc gccgggcgac ccgaccgctg gctgctgcgt atcccgcccg
     5701 gccatcccgc ggtggtgaac ccggcgaacg accacatccc cggcatggtg ctgctcgatg
     5761 cggcgcagca ggccgcccgc gtcctgaccg cgcccgatgc cttcgtcccg tacgccttcg
     5821 gcaccgactt ccgccggtac gccgagcacg gcaccccctg tgtgatcgaa gcccgccggg
     5881 tcccgtcggc gctgccctcc accaccacgg tgcaggtgac cggcctccag ggcggcgacc
     5941 cggtcttcgt ctccaccctg acggcactgg acccacgggg ctgacgacac cgctcaggga
     6001 gcaccgcgcc ggtcccgttt gccgccactg agcggcgggt caggcgtcgt ggaagaccag
     6061 gcccagggag tgccgccggc cggagcggac ggcgctcaca ccgtggcgta cgggaccggc
     6121 cgaccagccg cgggcggagc gtacgggccg gtctcgggtc gtgaagatca gtccatggcc
     6181 ctgcggaagg acggtgctgg tccctcggga ctgggcgcgc agccgctgct cgacgaggag
     6241 gaactcgccg ccggtgtagt cgatgccctg ctcgtccagg ccgatgacga cctggagggg
     6301 gaagaccatc tccccgaaga ggtcgcggtg cagcgcgttc cagtcacccg ccccgtaacg
     6361 cagcagaatc tgggaggact tggtctgccc ggcttcgtgg cacggggtca gccactcctc
     6421 cagggagtcc ggccagggag ccggcctgcc cagcttgtcc gcccagtcgc gggcgatctt
     6481 cagcaggtgc gggtagaacg cctcgcgcag ggcctggacc ggttcgggca gcgggttggc
     6541 gaagtagcgg tactggcccg agccgaagcg gtgccgtgcc atgtcgatgg tcgagcggaa
     6601 gcgggaccgg ccgccggatt ccgtgccctg cgcctcggcg ggctcgtcgt acagcgcgct
     6661 gagggcgcgg cagtcggccg ggctgagcag acgtggggtc accgcgcagc ccagggtgtc
     6721 cagttcctcg gccagcgccg gccagtcagc ggcggcgacg cgctgggcga gcgtacgggg
     6781 gcgggagtgg cgctcggtgg tcctggtggc tgtggtggtc gtggtggtca tggagcgctc
     6841 caggggcggg ggtcggtgcg gcgctcgggg gtcgcgggcg gcgcggggcg gctcagtggg
     6901 cctccggcaa ggcgccctcg atgccgagga gttgccgctt gcgtacgggc ccgcccgcgt
     6961 agcccgagag ggatccgtcc gcggcgatca ctcggtggca gggccggatg acgagcagcg
     7021 ggttcgcgcc gatggccgtt ccgatggcgc gtaccgcgcc tcgcgccgca ccgatgtcag
     7081 cggcgacctt cccgtacgtg gtcgtggtgc cgtacgggat ggcctccagc gcctgccaca
     7141 cccgccgctg gaagtcggtg ccccgggtgg gggcgcaggc aatggcgaag cgggtcagct
     7201 caccgtcgaa gtaggcgcgg agctggtcgg cgatgccgtc gaaggcggtg ggggcgtacg
     7261 tccagccgtc ctggacggtg gcgccgccct tctgcccggg tacggacagc gaggcgagcg
     7321 cgatgccacc cggcgcggtg gccgattcct cgcccaccag caggagttcg cccaaggggc
     7381 tgtcgagggt ggtgtagagc gtcatggtcc ggtgctttcc gtagtggtgg ggcaggggtt
     7441 cgccggctcc gtgagcggca cgttccagaa gtggtgcatc gcgtacgagc gccagggccg
     7501 ccaggtttcc gccagctccc gcagacccgc cgccggggcc ccggcccggc gcatgccggc
     7561 cagtacggcc acatcgccgg tcagcaggac atcggggtcc cccagggctc gcatccggat
     7621 gtatccggcg gtccacgggc cgatgccgcg caacccgagg agcgcgcgtt cggcctcgtc
     7681 ccggtcggct ccggcgtcca ggacgaccgt accgtcggcg agtgcgttgc tgagcgtgcg
     7741 cagggtcgcc cgccgtgact cgggcatccc gagttcggtg agggcggcct gcgcaaggtc
     7801 gtcgatacgg gggaagaggt gggtgagcgc accggccggt tcgggcagcg gttctccgta
     7861 ggcggcaacc agggcaccgc cgagggtgcg gccggccgcg accgagacct gctggccgag
     7921 tacggcacgg acggcgagct cgtccggggc gggggctcct ggcgagcgca gaccgggccg
     7981 ccggtccacc agccgcgcca gtacggcatc cgcgccgagc cgttcggcga ccgcgtacgg
     8041 atcggcgtcc aggtcgaaga ggcggcgcat ccgctggctc gcggtggtga gatcccgcag
     8101 ctcggccagg tgcagccggc agtccagcca gccgtcgccg gccgcctcgt cgacctcggc
     8161 gatcccggcc ccgtgcggca gccgcagggt gcgccggtag gtacgggcgc cgggctcgcc
     8221 gctgacttcc tcgatcccgg tgatcgcacg ccgctggagg tggtcgaaca gctcccgcgc
     8281 cgcaaacggg ccgcggaagg ccagtcgcag cggcagcacc ccgggggtgg tgggcgacgc
     8341 caccgggcca aaacgagtcg ccttaccggg gcgggcggca cgcagctcgc tcggggtgag
     8401 ggcgtagatc tccttgatgg tgtcgttgaa ctgccgcacg ctggcgaagc ccgccgcgaa
     8461 ggcgatctcg gccgcctgga gtccggtggt ctgcagcagg acccgggcgg tgtgggcgcg
     8521 ctgtgcccgc gccagggcca ccgggccggc acccagctcg gccgtcagct gccgctggac
     8581 ctgccgggcg ctgtagccga gccgggccgc cagcccggag acgccctccc ggtcgaccac
     8641 gccctcgctg atcagccgca tcgcccgccc gacgacatcg gcgcgggcat tccactccgg
     8701 cgagccgggc accgcatcgg ggcggcagcg ccggcaggca cggaatccgg agccctgggc
     8761 ggccgcggcg gtccggaaga aggcgacatt gcggcggcgg ggcgtggtgg cggggcagct
     8821 cggccggcag tagatcccgg tcgtggacac cgcgaagaag aaggcgccgt cgaatctggc
     8881 gtccctgctg cgcaccgcct cgtacctgct gtcctcgttc atcacagtgt ccactgtgca
     8941 gcaggtcagg cggcggcgct ggcggaaatc ggacggggcg ttcggggagc cgggaccgtg
     9001 cccggtccgc acgtgagcaa ctgaactccc aagggaggcc gacgcgcgtt cagggtttcc
     9061 ccggcgtcgg gcctgcccta cgaggccact accgtgagga tcacgagaat caggaggatc
     9121 acgagagtct cccagcgcat cgctctccgc aggacactca ggtccgaaga accgcccgac
     9181 ccgctaccgc gtgtcgagaa cacgccgtca tcactgctgt cgctgttccc gctgtcgtcg
     9241 ctcacgccct gccccgctcc ctgcccggct caccggccga cggcctgcct gccgatcgcc
     9301 ggacgtcagc gtatcccctg gtccgcggcg ccgagagacg ggtacggcca cggcgaacgg
     9361 ctgatcggcc ccggatgtca ggccgggggc atcggccagt cgtccagtga gcctccgcgc
     9421 cattcgacga ggcggggatc gtccagcgcg atactcccgg ccgggccggt gacgcccgcg
     9481 cggcggagaa attcggcaac atcgccgcgg ccatgggcca ggccgacgat ctgcccacgc
     9541 acggtcactc ggcggccacc ggacggcgtc ggcgggtgca cgatgacagg cggatgctcg
     9601 gacatgcatc cagcgtgcgc cgggctccgc gctcgcgcat ttcggcagcc gccgagtgcg
     9661 tcactccgcc atggaccatg ggcgacaatc agggccatga ccatggacga tcttgtgatt
     9721 cccgataccc gtgcctgtcg taccgcgctg gaggtggcga gcgcctactg ctcacccgcg
     9781 ctgctgaacc actccgtccg ggcgtatctc tgggcggccg gctacgcctc ggccaacggc
     9841 atcgccttcg atccggagct gctctacgtg tcagccatgt tccacgacat cgggctggtg
     9901 aaggagttcg acagccacac cgtgcccttc gaggaggcgg gtggccatgt ggcgtgggtg
     9961 ttcggggcgg gcgccggatg gccggtggag cgtcgggtac gggcgtccga ggtgatcgtc
    10021 cggcacatgt gggacgaggt ggacgcggcc atggatccgg aggcacatct gctggtgtac
    10081 tccacctcgt tggacatctc cgggcggcgt cccgacacgc tgccggacga cctccgcacc
    10141 gaggtgctgg cgcgctaccc acggctcggt ctcggtgaag agttcctggc gtgcttccgg
    10201 gaccaggccg ggcgcaagcc ggacagcacc gccgcggccg cggtccgcga gggcatcgcc
    10261 gcgcggatct ccgccaatcc gctggatgcg tacgggacgt agcgggcatc cgcaacgccc
    10321 ccgggcctgg cagccgtcgc agtcggccgc ggcaagagga agccccgacc agacggggga
    10381 agactggccg gggcggctcg ggaagggtgc gtcggcctct gcgtcaacac ccacacccat
    10441 ttgaacggtg aacaacgggg gcgcgttcca ggcgggcgac gtgatgacca tcacgccgcc
    10501 cgcgaggaac ggcacgcccc gcggcggctc agctcccggc gtggtctccg gagaggcgtt
    10561 cgacgccgcg caggagggcg gagtggtcga ggccgccgtc gccttgtgcg cggagggcgg
    10621 cgatgagttg ggcgacgagg ctgccgacgg ggagggtggc gccgacggtg cgggcggcgt
    10681 cggtgacgat gcccatgtcc ttgtggtgca ggtcgatccg gaagccgggg cggaagtcgc
    10741 gggtggtgaa gttgtccttc ttgcgggcca ggacggtgga gccggccagc ccgccaccca
    10801 ggacgtcgag ggcggcgctc aggtcgacgc cggacttctc caggaagacg acggcttcgg
    10861 cgcaggcctg gatgttgacg gcgacgatga gctggttggc ggccttgacg gtctggcccg
    10921 agccgtgcgg cccgcagcgc acgatcgtct tgccgagcgc ctccagcagc ggacgggcgg
    10981 cgtc
//
