LOCUS       NZ_CP114899            20747 bp    DNA     circular CON 18-FEB-2024
DEFINITION  Bacillus subtilis strain 1 chromosome, complete genome.
ACCESSION   NZ_CP114899
VERSION     NZ_CP114899.1
KEYWORDS    RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 4214774)
  AUTHORS   Li,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2022) College of Veterinary Medicine, Huazhong
            Agricultural University, Shizi street, Wuhan, Hubei 430000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v1.5 v. Hifiasm v0.12-r304
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/18/2024 04:35:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,515
            CDSs (total)                      :: 4,397
            Genes (coding)                    :: 4,195
            CDSs (with protein)               :: 4,195
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 9, 9 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 9, 9 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 128 of 202
            Pseudo Genes (incomplete)         :: 67 of 202
            Pseudo Genes (internal stop)      :: 46 of 202
            Pseudo Genes (multiple problems)  :: 34 of 202
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:13:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3910078
            Orig. end                         :: 3930825
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114899.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20747
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="11"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20747
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20747
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[10000:10747](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10747
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            1629..4502
                     /gene="uvrA"
                     /locus_tag="O6U12_RS20675"
                     /old_locus_tag="O6U12_20675"
     CDS             1629..4502
                     /EC_number="3.1.25.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_process="GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="uvrA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391396.1"
                     /locus_tag="O6U12_RS20675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20675"
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="WP_128738284.1"
                     /transl_table=11
                     /translation="MAMDRIEVKGARAHNLKNIDVTIPRDQLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAISIDQKTTSRNPRSTVGTVT
                     EIYDYLRLLYARVGKPHCPEHGIEITSQTIEQMVDRILEYPERTKLQVLAPIVSGRKGA
                     HVKVLEQIRKQGYVRVRIDGEMAELSDDIELEKNKKHSIEVVIDRIVVKEGVAARLSDS
                     LETALRLGEGRVMIDVIGEEELMFSEHHACPHCGFSIGELEPRLFSFNSPFGACPTCDG
                     LGMKLEVDADLVIPNQDLSLKENAVAPWTPISSQYYPQLLEAVCTHYGIDMDVPVKDLP
                     KHQLDKVLYGSGDDLIYFRYENDFGQIREGEIQFEGVLRNIERRYKETGSDFIREQMEQ
                     YMSQKSCPTCKGYRLKKEALAVLIDGRHIGKITELSVADALVFFKDLTLSEKDMQIANL
                     ILREIVERLSFLDKVGLDYLTLSRAAGTLSGGEAQRIRLATQIGSRLSGVLYILDEPSI
                     GLHQRDNDRLISALKNMRDLGNTLIVVEHDEDTMMAADYLIDIGPGAGIHGGQVISAGT
                     PEEVMEDPNSLTGSYLSGKKFIPLPPERRKPDGRYIEIKGASENNLKKVNAKFPLGTFT
                     AVTGVSGSGKSTLVNEILHKALAQKLHKAKAKPGSHKEIKGLDHLDKVIDIDQAPIGRT
                     PRSNPATYTGVFDDIRDVFAQTNEAKVRGYKKGRFSFNVKGGRCEACRGDGIIKIEMHF
                     LPDVYVPCEVCHGKRYNRETLEVTYKGKSISDVLDMTVEDALSFFENIPKIKRKLQTLY
                     DVGLGYITLGQPATTLSGGEAQRVKLASELHKRSTGRTLYILDEPTTGLHVDDIARLLV
                     VLQRLVDNGDTVLVIEHNLDIIKTADYIVDLGPEGGAGGGTIVASGTPEEITEVEESYT
                     GRYLKPVIERDKTRMKSLLKAKETATS"
     misc_feature    complement(4558..4569)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     gene            <4583..5147
                     /locus_tag="O6U12_RS20680"
                     /old_locus_tag="O6U12_20680"
                     /pseudo=""
     CDS             <4583..5147
                     /GO_function="GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /GO_process="GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017695304.1"
                     /locus_tag="O6U12_RS20680"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="O6U12_20680"
                     /product="flagellin"
                     /pseudo=""
                     /transl_table=11
                     /translation="RFRSELTVQAGNAGTQQAEDLGVIKDEMDALY"
     gene            complement(5346..5576)
                     /locus_tag="O6U12_RS20685"
                     /old_locus_tag="O6U12_20685"
     CDS             complement(5346..5576)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391394.1"
                     /locus_tag="O6U12_RS20685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20685"
                     /product="hypothetical protein"
                     /protein_id="WP_003235419.1"
                     /transl_table=11
                     /translation="MEKDPSDYTVTQESVLKLIHEQKRMNREMIAELEQIHGPFPISHD
                     IQYIKVLLDSSNTHIVQDLMSVSKQLYKKTL"
     misc_feature    5545..5560
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.68"
                     /tool="antismash"
     gene            5794..6120
                     /gene="yvlA"
                     /locus_tag="O6U12_RS20690"
                     /old_locus_tag="O6U12_20690"
     CDS             5794..6120
                     /codon_start=1
                     /gene="yvlA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391393.1"
                     /locus_tag="O6U12_RS20690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20690"
                     /product="protein YvlA"
                     /protein_id="WP_003244375.1"
                     /transl_table=11
                     /translation="MNRNQAIIASLCYFSVFIAPIIVPIVAYFVVNEKETKRHAIRSLI
                     SHIVPFVGWLFLFIALLGGAVAIDGDSLLPVFVIIGGAVIYFLVVIGIIIWNVIQGIKV
                     LRAA"
     gene            6145..7242
                     /gene="yvlB"
                     /locus_tag="O6U12_RS20695"
                     /old_locus_tag="O6U12_20695"
     CDS             6145..7242
                     /codon_start=1
                     /gene="yvlB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391392.1"
                     /locus_tag="O6U12_RS20695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20695"
                     /product="DUF4097 domain-containing protein"
                     /protein_id="WP_014478112.1"
                     /transl_table=11
                     /translation="MKQEKERILKLVEEGKLTAQEALTLIEKLDSDYKEKEEKITALSV
                     HVHDEEEPFTTAKKESGKPSLGAKLFDWIDSAVKKVKEVDLDLNFGHAYDVQHIFQFKD
                     TDFSSVELQIANGSVNIVPWEDDDIRAECQAKVYRADSQDAARHAFLQHIECEIKGNKF
                     FIRTEKKTMKTNVTLYIPQKEYDKIRVKLFNGPVRGEHLHVKEFSAKTTNGVLSFSHLT
                     AEKAIAETANGQIKLASHSCGTIEAETINGLIDLRGKSESIDVQSFNGNIAINVTESDC
                     RSIYAKTTTGSVELAIPDDLAVKAELKSNLGSLSHELMDVEMLKEKNDTIQKEMMFTSN
                     QAHDQNITVFSESLTGAIKLKYSQR"
     gene            7247..7444
                     /gene="yvlC"
                     /locus_tag="O6U12_RS20700"
                     /old_locus_tag="O6U12_20700"
     CDS             7247..7444
                     /codon_start=1
                     /gene="yvlC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391391.1"
                     /locus_tag="O6U12_RS20700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20700"
                     /product="PspC domain-containing protein"
                     /protein_id="WP_003228065.1"
                     /transl_table=11
                     /translation="MNKLYRSEKNKKIAGVIGGLAEYFNWDASLLRVITVILAIMTSVL
                     PVLLIYIIWIFIVPSERDMK"
     gene            7446..7805
                     /gene="yvlD"
                     /locus_tag="O6U12_RS20705"
                     /old_locus_tag="O6U12_20705"
     CDS             7446..7805
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene="yvlD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391390.1"
                     /locus_tag="O6U12_RS20705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20705"
                     /product="phage holin family protein"
                     /protein_id="WP_003228066.1"
                     /transl_table=11
                     /translation="MVKWAVSILVNALLLIVIDGYIDSIHISSIGAAIIASLILSILNV
                     LIKPLLIIFTLPVTMVTLGLFLFVINAITLMMTASIMGDSFQIDGFGTAIWASVILSVF
                     HLLIQKGILEPLRKK"
     gene            complement(7834..9045)
                     /gene="pchE"
                     /locus_tag="O6U12_RS20710"
                     /old_locus_tag="O6U12_20710"
     CDS             complement(7834..9045)
                     /codon_start=1
                     /gene="pchE"
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="O6U12_RS20710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20710"
                     /product="MFS transporter"
                     /protein_id="WP_017695294.1"
                     /transl_table=11
                     /translation="MAHTKSKAVLILYTVCFSAFFASLSQNIYSPILPIIKESFHVSTA
                     MVNLSVSVFMIVTAIMQIILGAIIDFKGARIVLITGILATAAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGDERGSAMGTYQMLLSVAPAIAPVLGGFIGGAAGY
                     EGIFWILAAISIVLLVTNSITFPKDSPTESMQQAKGNVFAHYKSIFTNRTGNVILTLSF
                     VLFFIYFAVIVYLPILLTEHYHIDVGIAGLLYLPLALSTIAGTFLFKRIQAKIGLHTLF
                     IGSNVFAACSIILFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GMFNFIRYTGMAAGPMVSAYLLTMMPSAISFSLLGLGFAALSFCLLPPMFSPQKRTKQK
                     KHHM"
     gene            complement(9066..9575)
                     /gene="pchR"
                     /locus_tag="O6U12_RS20715"
                     /old_locus_tag="O6U12_20715"
     CDS             complement(9066..9575)
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="O6U12_RS20715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20715"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_003228073.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVAEIPK
                     NMTSIHVIDCIGQHEPINNAGIARKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGRRIFDLHEKLHKKKELAFYQFLDSFSQEEQKAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     gene            10001..10747
                     /gene="pchC"
                     /locus_tag="O6U12_RS20720"
                     /old_locus_tag="O6U12_20720"
     CDS             10001..10747
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="O6U12_RS20720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20720"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_017695293.1"
                     /sec_met_domain="CDPS (E-value: 1.2e-82, bitscore: 274.8,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEMVTERRSVHFIAEALTENCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWAKAQFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     VAHGGDPKAIHTFSDFIDNKAYQLLRQEVEHAFFEQPHFRHACLDMSREAIIGRARGVS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIC
                     MRPNQGYLIVQEMAQMLSEKRITSEG"
     gene            10763..11980
                     /gene="cypX"
                     /locus_tag="O6U12_RS20725"
                     /old_locus_tag="O6U12_20725"
     CDS             10763..11980
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391386.1"
                     /locus_tag="O6U12_RS20725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20725"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_017695292.1"
                     /sec_met_domain="p450 (E-value: 6.4e-36, bitscore: 122.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQSIKLFSVLSDQFQNNPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFIGDALDHL
                     SPLIKQNAENLLAPYLERGKIDLVNDFGKTFAVCVTMDMLGLDKRDHEKISEWHSGVAD
                     FITSISQSPEARAHSLWCSEQLSQYLMPVIKERRVNPGSDLISILCTSEYEGMALSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEQPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKNEIEIVANIVLDKMRNIRLEEDFCYAESGLYTRGPVSLL
                     VAFDGA"
     gene            complement(12021..12494)
                     /gene="yvnA"
                     /locus_tag="O6U12_RS20730"
                     /old_locus_tag="O6U12_20730"
     CDS             complement(12021..12494)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="yvnA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="O6U12_RS20730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20730"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_017695291.1"
                     /transl_table=11
                     /translation="MDNSIHDELFQAIQQFALKRDKRVWQKVQIPSIGISSQHHDHLKK
                     DWTLTQLHIVSCIHTSQNVNNSFLASRLHISKAAVSKAVHALLKHNIITVTKKPGNKKE
                     IFYTLTDSGRKLAALHEQLHEKAKEQYKQLFNEFSIDDLKTVTAFLNLWIKYM"
     gene            12659..16528
                     /gene="yvnB"
                     /locus_tag="O6U12_RS20735"
                     /old_locus_tag="O6U12_20735"
     CDS             12659..16528
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="yvnB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="O6U12_RS20735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20735"
                     /product="metallophosphoesterase"
                     /protein_id="WP_269767320.1"
                     /transl_table=11
                     /translation="MRKYTVIASILLSFLSVLSGGHHELKAVPVVQQELFQTPHYIPLL
                     ENPPQIDVKAEMRNNLMIEAQVRDNSYQAFSAFLFYKQSNELGYKMVPMDPTPGAVRKF
                     LAQIPKRLIWNSELEYYIVMSNGKQRVESETKKLKLEGYQADLAHIPELLITELAVDTK
                     NTGRADGYEFIEIYNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERIIIPSGETFVV
                     WLKPAGHPELTSEDFNRYYQTQLKEGKNLAVIDETEGMANTRPRAVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLNGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKHVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILAEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKAHGLRLNIENRDTLSGTK
                     FLKATTEGRTDRIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTINKYRTYSVPLPETLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLEWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEAEASLDGESI
                     TLPYHTSSSDLLPGKHSLKVTATDLAGNKTVIERIFKTEREHPDQPEVIDSEADTHKAK
                     LSVRVKDPTNDAMDVGFYRGFQYTARNHVKIFKHASLTEPPKSFVPERETLFTNKELER
                     VSAADGKTVSTENKELFPYHRFEVTVDPSIDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDDKPFTLKASMIAADFVRESKMNVIVQDEIPPAKDMYTFVWMSDTQY
                     YAESYPHIFDKQTEWIKDNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSDRFDKKLHFAGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDEDIAWMNQVLKKHPDRMAILAFHEYLLVSGNRSAIGEKIFKEIVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPYVKDKNYYDTDTYGNKDEFSLSLDLKPRIKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDHLESQSVYYWYIIVEDSFNGKTKSPIWKFKTKKETYRPAPDQFDFR
                     HVSNP"
     gene            complement(16548..17279)
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="O6U12_RS20740"
                     /old_locus_tag="O6U12_20740"
     CDS             complement(16548..17279)
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="O6U12_RS20740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20740"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_014478108.1"
                     /transl_table=11
                     /translation="MNINKQSPIPIYYQIMEQLKTQIKSGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGLLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIDSTEELAAILGCGHPSSIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYDHIERYNSIPISRAKQELEPSAATTEEANILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(17297..18025)
                     /gene="nagB"
                     /locus_tag="O6U12_RS20745"
                     /old_locus_tag="O6U12_20745"
     CDS             complement(17297..18025)
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="O6U12_RS20745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20745"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_017695289.1"
                     /transl_table=11
                     /translation="MKVMECQTYEELSQIAARITADTIKEKPDAVLGLATGSTPEGTYR
                     ELIRLHQTENLSFQNVTTVNLDEYARLSSNDPNSYHFYMNDRLFQHIDSKPSRHFIPNG
                     NADDLEAECRRYEQLVESLGGTDIQLLGIGRNGHIGFNEPGTSFKSQTHVVTLNEQTRQ
                     VNARYFPSIDSVPKKALTMGIQTILSSKRILLLISGKSKAEAVRKLLEGNISEDFPASA
                     LHLHSDVTVLIDREAASLRP"
     gene            complement(18022..19212)
                     /gene="nagA"
                     /locus_tag="O6U12_RS20750"
                     /old_locus_tag="O6U12_20750"
     CDS             complement(18022..19212)
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="O6U12_RS20750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20750"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_017695288.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.5e-42, bitscore:
                     142.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKDIAIVTENEVIKNGYVGINDGKISTVSTERPKEPYSK
                     EIQAPANSVLLPGMIDIHIHGGYGADTMDASFSALDIMSSRLPEEGTTSFLATTITQEH
                     GNISQALVNAREWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIRHLKDESIIASMGHTDADSALLSDAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGRTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTVGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     misc_feature    complement(19267..19282)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            19394..20326
                     /gene="hprK"
                     /locus_tag="O6U12_RS20755"
                     /old_locus_tag="O6U12_20755"
     CDS             19394..20326
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="O6U12_RS20755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O6U12_20755"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_017695287.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGINRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEDKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQEE"
ORIGIN
        1 gagacactcc gccacatcag ctttgtttga gcgcagagat gtcattatta ttgcgagtgt
       61 gtcttgtata tacggcctcg gttcccctga ggaataccgg gaaatggtcg tgtcactgcg
      121 gactgaaatg gaaattgagc gcaacgagct gctcagaaaa cttgtagaca tccaatatgc
      181 ccgcaacgat atcgactttc agcgcggtac atttcgtgtg cgcggggatg tagtggaaat
      241 ctttccggca tcccgtgatg agcattgtat tagagttgaa tttttcggtg acgaaatcga
      301 acggattcgg gaagtggatg cgctgacagg agaaattctc ggcgaccgag atcatgttgc
      361 gattttcccg gcatcccact tcgtaacgcg ggccgagaaa atggagaagg cgattcaaaa
      421 tatcgagaag gagcttgagg agcagctgaa ggtcatgcac gaaaacggaa agctccttga
      481 agcgcagcgt ttagagcagc ggacaagata tgatcttgaa atgatgcgtg aaatgggttt
      541 ctgctccggc atcgagaact attcaagaca tttgactctg cggcctccag gttcaacgcc
      601 gtatacgctt cttgattatt tcccagacga ttttatgatc gtggtggatg agtcgcatgt
      661 gacaattcct caggtgcgag gcatgtttaa cggagaccag gcgcggaaac aggtgctcgt
      721 ggatcacggc ttccggcttc catcagcgct tgataaccgt ccgcttcgtt ttgaggaatt
      781 tgaaaagcat atgcacaata tcgtgtatgt atcagcaaca ccagggccgt acgagattga
      841 acatacagat gaaatggtcg aacagatcat tcgtccgacg gggcttcttg acccgcttat
      901 tgacgtccgt ccaatcgaag gtcagattga cgacttgatc ggcgaaattc aagcaagaat
      961 cgagcggaat gaacgggtgc ttgtgacaac tttgacgaag aagatgtcag aggatctgac
     1021 caattattta aaggaaatcg gcattaaagt aaactatctg cattctgaga tcaagacgct
     1081 tgaacggatt gaaattatcc gcgacctgcg ccttggcaag tacgatgtgc ttgtcggcat
     1141 caacctgctg agggaaggtt tggacattcc cgaagtatcc cttgttgcga ttttggatgc
     1201 ggataaggaa ggtttccttc gttcggagcg gtcgctgatc cagaccatcg gacgggcagc
     1261 gagaaatgcg gaaggccgcg tcattatgta cgccgataaa ataacgaagt ctatggaaat
     1321 tgcgattaat gaaacaaaac gccgccgcga gcagcaggag cggtttaacg aagagcacgg
     1381 cattacgccg aaaacgataa ataaagaaat tcgcgatgtc attcgtgcca cagtagcagc
     1441 tgaggataaa gcggaataca aaacaaaagc cgcgccgaag ctgtcgaaaa tgacgaagaa
     1501 agaacgccag aaagtcgttg aacagatgga gcatgaaatg aaggaagccg ccaaggcgct
     1561 tgactttgag agagccgcgg agcttcgcga tttactttta gagctaaaag cggaaggatg
     1621 atgaacaaat ggctatggat cggatagagg tgaagggagc cagggcgcat aacctgaaaa
     1681 atatagatgt aacgattccg agagatcagc ttgtcgttgt cacgggtttg tccggatcag
     1741 gtaaatcctc ccttgccttt gacacgatat atgctgaagg acagagacgg tatgtcgagt
     1801 cgctgtctgc ttatgcccgc cagtttttag ggcaaatgga taagccggat gtggatgcaa
     1861 ttgaggggct ctctcccgcc atcagcattg atcagaaaac aacgagccgc aatccgaggt
     1921 ctactgtcgg tacggtaact gagatttatg attatctgcg tcttttatat gcgagagtag
     1981 ggaagcctca ttgtccggag cacggaattg agattacatc ccagaccatc gagcaaatgg
     2041 tggacagaat tctggaatac ccggaacgga cgaagcttca ggtgctggcg ccgattgtct
     2101 cgggccgaaa aggcgctcat gtcaaagtgc ttgaacagat tagaaaacaa ggctatgtca
     2161 gagtcagaat tgacggcgag atggctgagc tttctgacga tatcgaatta gaaaagaaca
     2221 agaagcattc cattgaggtt gtcattgacc ggattgtggt gaaagaaggc gtggcagccc
     2281 ggctgtcaga ttcattggaa acggcgcttc gtttaggtga aggacgggtt atgatcgatg
     2341 tcatcggtga ggaagagctg atgttcagcg agcatcatgc ctgtccgcac tgcggatttt
     2401 caattggtga gcttgagccg cgtctgtttt cgtttaacag tccgttcggg gcgtgtccga
     2461 cgtgtgacgg tctcggaatg aagcttgaag tggatgccga tcttgtcatc cccaatcaag
     2521 atttgtcatt gaaggagaat gcggtcgccc cttggacacc gatcagctca caatattatc
     2581 ctcagctgct tgaggcagtc tgcacccact acgggattga tatggatgtg ccggtcaaag
     2641 atttgccgaa gcatcaactg gataaagtgc tgtacggcag cggggatgac ctgatttatt
     2701 tccgatatga aaatgatttt ggacaaatac gcgaaggtga aattcaattt gaaggcgtat
     2761 tgcgcaacat tgaaagacgc tataaggaga caggctccga tttcatccgt gagcaaatgg
     2821 agcagtatat gtctcagaaa tcttgtccga cgtgcaaagg ctatcggtta aagaaagagg
     2881 cgcttgccgt actgattgac ggccgccaca ttggaaaaat taccgagctg tctgtcgccg
     2941 acgcacttgt cttctttaaa gatctcaccc tttctgagaa ggatatgcag atcgccaatt
     3001 tgattttgcg cgaaattgtg gagcgcttaa gctttctgga caaagtcggc ctcgattacc
     3061 tgacattgag cagggcagcg ggtacattgt ccgggggaga ggcgcagcgc atcaggctgg
     3121 cgactcaaat tggctcgcgt ttatccggtg tgctttatat tttagatgag ccgtctatcg
     3181 gtctgcatca gcgtgataac gaccgcttga tcagcgctct gaaaaatatg agagacctcg
     3241 ggaacacgct gattgttgtc gaacatgatg aggacacgat gatggcagca gattatttaa
     3301 tagatattgg accgggagcc ggcattcacg gcggacaggt gatatctgcg ggtacgccgg
     3361 aagaagtgat ggaagatcca aactcattaa cgggcagcta tttatcaggg aaaaagttta
     3421 tcccattgcc tcctgaaaga agaaagccgg acggacgtta cattgaaatt aaaggtgctt
     3481 cagaaaacaa cctgaaaaaa gtgaatgcca agttcccgct tgggacgttt acagcagtta
     3541 caggtgtttc cggttcagga aagagtacac tcgttaatga aattttgcat aaggcgctgg
     3601 cgcaaaagct tcataaagcg aaagcgaagc ccggaagcca taaagagatt aaaggtttgg
     3661 atcatttaga taaagtcatt gacattgacc aggcgccaat cggaagaacg ccgagatcca
     3721 accctgcgac atacaccggt gtatttgatg acattcgtga tgtattcgcg cagacaaatg
     3781 aagcgaaggt ccgcggctat aaaaaaggcc gtttcagctt caacgtgaag ggcggacgat
     3841 gtgaagcctg ccgcggagac gggattatta aaattgaaat gcacttcctt cctgacgtat
     3901 acgttccatg cgaggtgtgt cacggcaaac gctataaccg tgaaacgctt gaagtgacgt
     3961 acaaaggaaa aagcatctct gatgtgcttg atatgacggt tgaagatgct ctttctttct
     4021 ttgaaaatat cccgaaaatc aaacgcaagc tccaaaccct ttatgatgtt ggtttaggtt
     4081 atattacgct cggccagccg gcgacgacct tgtcaggcgg agaagcgcag cgcgtgaagc
     4141 tcgcatcaga gctgcacaaa cgctcgaccg gacgcacgct ctacatttta gatgagccga
     4201 cgacaggttt gcatgtcgac gatatcgcca ggcttcttgt cgtgctgcaa cggttggtag
     4261 acaacggaga cactgtactg gttattgagc ataatcttga tatcattaag acggccgatt
     4321 acattgtgga tttgggcccg gaaggcggag ccgggggcgg aaccattgtc gcgtcaggaa
     4381 cgcctgagga aatcactgaa gtggaagaat cgtatacagg ccgttatttg aagcctgtta
     4441 tcgaacgtga caaaacacgc atgaaatcgc tcttaaaagc gaaagaaaca gctacatctt
     4501 aaaaccctct gttaagaggg gacagcttgt cagcaagtcc atccttgggc ttagcaggaa
     4561 agctttttct ttacggcata ataggtttag aagtgaactt actgttcaag caggaaacgc
     4621 aggtacacaa caagcagaag atcttggtgt aattaaagat gaaatggatg cgctctattg
     4681 aggaagttga tggcatttca aatcgtactg aattcaacaa caaaaagtta ttagatggta
     4741 ctgaaactga tggtttcact ttccagattg gagcaaatgc tggccagtag cttacaatta
     4801 acattgacag catgtcatca actgctttag gagtaaacgc acttaatgta acaaattttg
     4861 ctaatactcc ttttgatact caacttgaaa gtattgatac tgcaatcaac aatgtctcaa
     4921 accaacgtgc taaacttggt gcggtacaaa accgtctaga gcacacaatc aacaaccttg
     4981 gtgcttcttc tgaaaactta acagctgctg agtctcgcat ccgtgacgtt gacatggcga
     5041 aagaaatgag cgatttcaca aagaacaaca ttctttctca agcttctcaa gcgatgcttg
     5101 ctcaagcaaa ccaacagccg caaaacgtgc ttcaattgtt acgttaattt tgaacggcta
     5161 tctctttctg atagttacag cttgccgaca gagtccatta actggatgtg ccggcaggct
     5221 ttttttgtta caatcatttt gtgacagcca gggaatcact tgttttttca aaacaaaaaa
     5281 ggcaagatat tgtcatctaa cccttaactt tgtttcagtt ataatgaatg atatgttttc
     5341 cgcaattaca aagtcttctt atacagctgc ttacttacac tcattaaatc ttgaacaata
     5401 tgcgtgttgc tgctgtcgag cagcactttt atatattgga tatcgtgaga gatcgggaag
     5461 ggtccgtgaa tctgctcaag ctcggcgatc atttcacgat tcattctttt ttgttcatga
     5521 atcagcttta acactgattc ttgtgttact gtatagtcac tgggatcttt ctccattgct
     5581 tgtccagcct ccttggttgc cgtaagcttt ttacatattg taatcggcat agccggaaga
     5641 ttctacttct aaaacttttt aaaatccaat ttccgtaaat cggagcatgt ctaaagaatg
     5701 aatttgaaac ctgaagagat tttaaacgta taaataagta aaacgtccct gatcttttgt
     5761 catggacaat tcacaatagg agtgtggaat gaattgaacc gtaatcaagc tattattgca
     5821 tcactttgtt atttcagtgt gtttattgcg ccaatcattg tgccgattgt cgcttatttt
     5881 gttgtaaatg agaaagaaac aaaacgccat gcgatacgct ctcttatttc tcacatagtt
     5941 ccgtttgtcg gctggctgtt cctttttatt gctttacttg gcggagccgt tgcgattgat
     6001 ggagacagct tgcttcctgt atttgtaatc atcgggggag cggttattta tttcttagtt
     6061 gtcattggca ttatcatttg gaatgtgatt cagggcatta aggtcctgcg tgcagcataa
     6121 gtagacaatg gggaggagat cgtaatgaag caagaaaagg aacgaatcct taagcttgtt
     6181 gaggaaggaa agcttacagc acaagaagct ttgacactga ttgaaaagct tgatagcgac
     6241 tacaaggaaa aagaagagaa aatcacagcg ctctcagttc acgttcatga cgaggaagag
     6301 ccatttacaa cggcaaaaaa agaaagcggc aagccgtctt taggagccaa gctttttgac
     6361 tggattgatt ctgctgtgaa aaaggtcaag gaagttgacc ttgatttaaa ctttggccat
     6421 gcgtatgatg ttcagcacat ttttcaattt aaagatactg acttttccag tgttgagctg
     6481 caaattgcca acggcagcgt gaatatcgtc ccttgggaag atgacgatat cagagcggaa
     6541 tgccaggcga aggtatatcg cgctgacagc caggatgctg cgagacacgc atttcttcag
     6601 catattgaat gtgaaatcaa aggaaataaa ttttttatcc gtactgaaaa aaagacgatg
     6661 aaaacgaatg tcactttgta tattcctcag aaggagtatg ataagattcg agtgaagctg
     6721 tttaacggtc cggtcagagg ggaacattta catgtaaaag agttctcagc gaaaacgaca
     6781 aacggcgtgc tgtcgttctc gcacttaaca gccgaaaaag ccatagctga aacggccaac
     6841 gggcaaatta aactcgccag ccacagctgc ggaacgatag aagcggaaac cattaacgga
     6901 ttgatcgatc tccgagggaa aagcgaatcc attgacgttc aaagctttaa cggaaatatt
     6961 gcgattaacg taacagaatc agactgccgc tccatttatg caaaaacaac gactggaagt
     7021 gtggagcttg cgattccgga cgatctcgca gtgaaggcag agctgaaaag caatcttggt
     7081 tcgctgtccc atgaattaat ggatgttgag atgctgaagg aaaaaaatga taccattcaa
     7141 aaagaaatga tgttcacttc aaatcaggca catgatcaaa acatcaccgt cttctcagaa
     7201 tcactaacag gcgccatcaa actaaagtac tcacagaggt gatcgaatga ataagcttta
     7261 tcgctcagaa aaaaataaga aaatcgccgg tgttatcgga ggtctcgcgg aatatttcaa
     7321 ttgggacgca tcactcctca gagtcataac tgtcatctta gccattatga caagtgtctt
     7381 gccggttttg ctcatttata tcatttggat ttttatcgtc ccgtcagaaa gggatatgaa
     7441 atgacatggt aaaatgggca gtcagcattt tagtcaatgc attgttatta atcgtcattg
     7501 atggatatat tgactccatc catatcagca gcattggggc agcgattatc gccagcctca
     7561 ttttatcgat cttaaacgtg ctgattaaac cgcttttaat tatctttaca ctgccggtca
     7621 ccatggtgac gcttggactc ttcttgtttg tgattaacgc catcacgctt atgatgaccg
     7681 cgtccattat gggtgacagc tttcaaatcg acggcttcgg cacagcgata tgggcatccg
     7741 ttatcctgtc ggtcttccat ttgctgattc aaaaaggcat tttagagccg cttagaaaaa
     7801 aataaaaaaa agctgcccgc aaaggcagct tttttacatg tggtgctttt tttgtttcgt
     7861 gcgcttctgc ggcgaaaaca ttggcggaag aaggcaaaag ctcaatgcgg caaatccaag
     7921 gcctaggagg ctaaaggaaa tggcagacgg catcattgtg agcaagtagg cagataccat
     7981 cggacctgct gccatgcctg tatatcggat aaagttaaac attccgattg cactccctct
     8041 gttgtgctca aattcattag taatcattgt agagtacaag ggaggaataa cccccatcga
     8101 gatgccaaac agtgccagcg tcagagccat gagaacgaga gaaacggaat gtgtaacagc
     8161 aaataaaatg atgctgcagg cggcaaacac attgcttccg ataaacaagg tgtgcagccc
     8221 gatttttgcc tgtattcttt taaacagaaa cgtacctgca atcgtgctca gcgccagcgg
     8281 caaatataac agtcctgcta tacccacatc tatatggtaa tgctctgtca gcaatattgg
     8341 gaggtagaca attactgcaa aataaatgaa aaagagaaca aaacttaaag tcaaaatgac
     8401 gttccctgtt cgatttgtaa atattgattt ataatgagcg aacacattgc ctttggcttg
     8461 ctgcatagat tcagttggag aatctttagg aaaggtgatg ctgtttgtca ccagcaaaac
     8521 gatcgagatg gccgcaagta tccaaaaaat cccttcgtat ccggctgctc cgcctatgaa
     8581 tcctcctaga actggggcaa tagccggtgc gacagacagg agcatttgat acgttcccat
     8641 tgcactcccg cgttcatcgc ctgtaaacag ctgtccgatc gttgtggcag caataagagg
     8701 cagtgctgcg gaaccggctg cctgtatcat tctgaatatc agaaacaaag taaagtcagt
     8761 agtcaccgca cagccgatgc tggctgctgc cgttgccaga ataccggtaa tcaagacgat
     8821 ccgagcgcct ttaaaatcaa tgatcgctcc taatataatt tgcattattg ctgtcacaat
     8881 cataaaaact gagactgaca ggttcaccat agctgtggaa acatggaatg attctttaat
     8941 gatcggaaga ataggtgaat aaatgttctg gcttaaagat gcaaaaaatg cactgaagca
     9001 aacagtgtat aagatcaata ctgcctttga ttttgtatga gccaaagtgt acaccgcctt
     9061 tttgtttact ttacaggttt gtccggagtc ccatcggttt gttctgcttc aagtgttgac
     9121 gtcaactgct ctagaaactt caatacagcc ttttgttctt cttgtgaaaa tgaatcgagg
     9181 aattggtaaa aagccagctc ctttttttta tgcagtttct catgtaagtc gaaaatccgt
     9241 ctgcctttac gggttaattt aaaataaact tcttttttgt tatctgtcag ctgatagctg
     9301 ttaatgaatt cttccttgat cagttttgtg ctgattttcg ttacattcgc tttcgataag
     9361 ttcatttttc tggcaatccc cgcattgtta atgggttcat gctggccgat gcagtcaatg
     9421 acgtgaatgc ttgtcatatt tttgggaatt tcagctacat cctcttctgc ggcctcctta
     9481 aaaaattgct gaagtgaagt gttcgctttt tgttcattaa ggtgcagcag tctcacgtat
     9541 atgtcgtata tcatctgttt tgtcaaatca gacataggct accttctttc ttaggaataa
     9601 tagtttacga tgaaacaata aaacaaatcg tttactggtg aacaatttca ttttatcttt
     9661 tcttgttttc gttgtcaatt gttggtgttt agactatttg ttgatatgtc atgctgaata
     9721 tgcgtgtaaa aagtgtgtgg aaaatgtgat gtttacattt attttcaaat atttgggcat
     9781 agatcatatt gacgtttgga aggaaaaggg ataaaatgac taatgtttac tagtaaacat
     9841 ttatcatgat atgtgaaatg gaaatagtta ttaaaatttt aaaaaacatt tggaaacttt
     9901 aagtgctgcc tgccattcgt cttgttcagt tgttctagct attccatctt gtatttgagg
     9961 gtgatgactt aaagcgggat aagttttagg ggtgaatgag atgaccgaaa tggtaacgga
    10021 aagaaggtct gtgcatttta ttgctgaggc attaacagaa aactgcagag aaatatttga
    10081 acggcgcagg catgttttgg tggggatcag cccatttaac agcaggtttt cagaggatta
    10141 tatttacaga ttaattggat gggcgaaagc tcaatttaaa agcgtttcag ttttacttgc
    10201 agggcatgag gcggctaatc ttctagaagc gcttggaact ccgagaggaa aggctgaacg
    10261 aaaagtaagg aaagaggtat cacgaaacag gagatttgca gaaagagctc ttgtggctca
    10321 tggcggggat ccgaaggcga ttcatacatt ttctgatttt attgataaca aagcctacca
    10381 gctgttgaga caagaagttg aacatgcatt ttttgagcag cctcattttc gacatgcttg
    10441 tttggacatg tctcgtgaag cgataatcgg gcgtgcgcgg ggcgtcagtt tgatgatgga
    10501 agaagtcagt gaggatatgc tgaatttggc tgtggaatat gtcatagctg agctgccgtt
    10561 ttttatcgga gctccggata ttttagaggt ggaagagaca ctccttgctt atcatcgtcc
    10621 gtggaagctg ggtgagaaga tcagtaacca tgaattttct atttgtatgc ggccgaatca
    10681 agggtatctc attgtacagg aaatggcgca gatgctttct gagaaacgga tcacatctga
    10741 aggatgatag ggggagtaaa acatgagcca atcgattaaa ttgtttagtg tgctttctga
    10801 tcaatttcaa aacaatccat atgcttattt ttcacaactg cgggaggaag atccggttca
    10861 ttatgaagag tcgatagaca gttattttat cagccgctat catgatgtcc gctatatcct
    10921 tcagcatccg gatatcttca cgacgaaatc acttgttgag cgtgccgaac cagtcatgcg
    10981 aggccctgtg ctggcccaaa tgcatggaaa agaacactct gccaaaagaa gaattgtagt
    11041 gagaagcttt atcggtgacg cactggatca tctgtctcca ttgattaaac aaaatgcaga
    11101 aaacttgtta gcgccttatc ttgaaagagg gaaaattgat ctcgtcaatg attttggaaa
    11161 gacattcgcg gtgtgcgtca cgatggacat gctcgggctg gataaaagag accatgaaaa
    11221 aatctctgag tggcacagcg gagttgccga ttttatcacg agtatctctc aatctcctga
    11281 agcgcgggca cattcgttat ggtgcagcga acagctttcc caatacttga tgccggtcat
    11341 taaagaacgt cgcgtcaatc cgggatcaga tttaatttcg atcctatgta cttctgaata
    11401 tgaaggcatg gcgctgtcgg acaaggatat actcgcactg attcttaatg tgctgttagc
    11461 cgcaacggaa ccggctgata agacgctggc actgatgatc taccatttgc tcaacaatcc
    11521 tgagcagatg aatgatgttt tggctgaccg ttcgttagtt ccgagagcca ttgcggagac
    11581 attgcgttat aaaccgccgg ttcagctgat tccgcggcag ctgtcccaag atacagtggt
    11641 cggcggtatg gaaatcaaaa aagatacgat tgttttttgt atgatcggtg cggctaaccg
    11701 ggaccctgaa gcatttgaac agcctgacgt gtttaatatt catcgggaag atcttggtat
    11761 caagagcgct tttagcggcg ccgcccggca tctcgctttc ggatccggca ttcataactg
    11821 tgtaggagca gcttttgcca aaaacgaaat cgaaattgta gctaatattg tgctggataa
    11881 gatgcggaat atcagattag aggaagattt ttgttatgct gagtccggtc tgtatacacg
    11941 cggacctgtt tcacttctcg ttgcgtttga cggggcataa tagaattcca aaggtctctc
    12001 ccatgcgggt gagactcttt ttacatatat ttgatccaca agtttaaaaa cgcagtcact
    12061 gtttttaaat catcaattga aaattcatta aaaagctgtt tatattgctc ctttgctttc
    12121 tcatgaagct gttcatgtaa tgccgccaat tttctgccgc tatcagttag cgtataaaaa
    12181 atttcttttt tgttgccagg cttttttgtc actgtaatga tattgtgttt caacaaagca
    12241 tgaaccgctt ttgaaacagc ggcttttgag atgtgtaaac gggatgctaa gaagctgttg
    12301 ttcacatttt ggctggtatg tatgcaggaa acaatgtgaa gctgggtcaa agtccagtct
    12361 tttttgaggt gatcgtgatg ttgtgaggat atgccaatgg atggtatctg caccttttgc
    12421 cagacgcgtt tatccctttt taatgcgaat tgttgtatag cttgaaataa ttcatcatga
    12481 atgctgttgt ccatcaattt gcctctctga tttaattgtt aactggttaa ctatccttag
    12541 tttactgggt aatagtggaa ttttcaagag acagacgttt ttttgttctt tatgcacaaa
    12601 aatgaactca taagcttccc tgttcagacg caaaatacat gaaaagggag gtgctttgat
    12661 gaggaaatac acggttattg cttctatatt actgtctttt ctttccgttt tgtctggcgg
    12721 ccatcatgaa ttgaaagctg ttcctgttgt acagcaggaa ctatttcaaa cgcctcatta
    12781 catacccttg cttgaaaatc ctcctcaaat tgatgtgaaa gctgaaatga gaaacaatct
    12841 aatgattgag gctcaagttc gcgataattc gtatcaagca ttttccgcct ttctttttta
    12901 taaacaaagt aatgagctgg gctacaaaat ggtgccgatg gacccgaccc ctggagcggt
    12961 ccgcaagttt ttggcacaaa ttccgaaacg tttgatttgg aacagcgagc ttgagtatta
    13021 cattgtgatg tcgaatggga agcagcgggt cgagtcggag acgaagaaac tgaaacttga
    13081 aggatatcag gctgatcttg cccatattcc ggagcttctg atcacagagc tagcggttga
    13141 tacgaagaac acgggacgtg cagacggtta tgaatttatt gagatttata atacgactga
    13201 cagaacgatt gattttaaag actatcatat ccgttaccgc tatccgaaag aagggcctga
    13261 ttccgatctg atttggagac cggatgaaag gatcatcatt ccatctggcg aaacgtttgt
    13321 cgtctggctg aagcctgccg ggcatccaga attgacgtca gaggatttca atcgttatta
    13381 tcagacacaa cttaaagaag gaaaaaatct cgcggtgata gatgagacag agggtatggc
    13441 caatacgagg ccgcgggccg ttgtgatatc aacgaataca ggaaaggaca tttcggttgc
    13501 gcattatcgg aaacatgcgc tgcgccggct gtcatcagtg ctttataagt atccgttgaa
    13561 cggaaccgca gaacttctga acatttcaat cggggaaaag aatccgtctc ccggaaccgt
    13621 attacaggca caggtgcccg agcagaaacg gaaaatcaag ctcgataaag aaaaaccggt
    13681 cattgaggat ttgactgacc gcaagcatgt ccgccctgct gaaagtattg agctgcgggc
    13741 tgacatacgg gaccgaagtc tagtgaaaac agcggccttt tactaccgga cagatgagaa
    13801 caaaccgttt aagcgaattc ttgctgaaaa agaccgtaac gataatctct accattatat
    13861 tgtgtattcg cctgaattga tcgggaagga tcaactagag tattatgtgg cggccgggga
    13921 cgggatcaat gaagcgagaa cgccggtcaa aatgattgat attaaacaaa caagcaaggc
    13981 gcacggtctg cggttgaata ttgaaaacag agacacgctg tcgggaacta aatttctcaa
    14041 agccacgaca gaagggcgaa ctgaccgcat taagctgtgg attgacggga aaaaacaagt
    14101 gacagagccc gcaatggaaa aagaagtata ttttgcattc gatacgagga aaacgaacct
    14161 ttattttaaa aacgcggtaa cgatggaagg caaagtgctg aaggtgttcg atgatacaat
    14221 aaataaatac cgcacctatt ctgttccgtt gccagagaca ctgcttcgga aagggaagca
    14281 gctgcagcgt attacaatac gatcgggttc gaaggtgtct ccgtttgata cggctgaaaa
    14341 ccgtgatgac ttcttagtca aaaacgcccg attggtgtta tcggatggaa cggtgatcag
    14401 ggatcagagg gtgtcccctg agaaggaact ctttattggc gataatcagc gctcgaataa
    14461 aagctggcaa tttcagttca atctgcctga cggactattt acatcacagc tgctggagtg
    14521 ggacacatca aagctttctg aaggcgctca ccatattcaa gcaagcgacg gcaaggaaaa
    14581 tgtcagcctg gttgtacggg tagataattc aggacctcac attgagccta atatcaccga
    14641 ggggcaaacg tataaaggaa accttatcct gcaggcggat atgtatgata aatggagcaa
    14701 aattgaggaa gcggaagcgt ctttagatgg agaaagcatt acactcccat atcatacgtc
    14761 atcttctgac cttttgccgg gaaaacattc tctaaaagtg acagcaacgg atctcgcggg
    14821 caataaaaca gtgattgaga ggatatttaa aaccgaacga gagcatccgg accagccgga
    14881 ggttatagac agcgaagccg acacgcataa ggccaagctt tctgtacggg tgaaagatcc
    14941 gacgaatgat gccatggatg ttggttttta taggggattt caatatacag cgcgcaatca
    15001 tgtgaaaata tttaagcacg cgtcactgac tgaaccgccg aaaagctttg tgccggaaag
    15061 ggagacccta tttacgaata aagaattgga gcgggtatca gctgctgatg gaaaaacagt
    15121 atcgacagaa aacaaagagc ttttccctta tcatcgcttt gaagtgacgg ttgatccatc
    15181 aattgatgag aatgatctgg cagaagcagt ctggaagggc agttctttgc cggggagaaa
    15241 ggtgacgatg tatgcttgga attatcggac aaacgaatgg cagcctgttg acagcttcgt
    15301 cgcgaaggat gacaaaccgt ttacgctgaa agcgtctatg attgccgccg attttgtacg
    15361 ggagtcgaaa atgaatgtga ttgtacagga tgaaattcct cctgcgaagg atatgtacac
    15421 atttgtctgg atgtctgata cacagtatta cgccgaaagc tatccgcata tttttgataa
    15481 acagaccgaa tggataaaag ataatcaaaa gcagctcaat atcaaatatg tgttccacac
    15541 gggtgatatt gtagatgatt ccgctgatat cagacagtgg aaaaacgctg atcggtcaat
    15601 gtccgttctg gataagagcg gaatacctta cggcgtgctg gccggcaacc acgatgtggg
    15661 gcataaggac ggctcctaca gggcgtttgg caaatatttt ggcagcgatc gttttgataa
    15721 aaaattgcat tttgccggtt cctataaaaa taaccgtgga cattatgatc tgatttctag
    15781 caacggcaat gattatatca tgctgtacat gggatggggc attacggatg aagacatcgc
    15841 gtggatgaat caggtgttga agaaacaccc tgaccggatg gccattttag cttttcatga
    15901 gtatttgctg gtcagcggaa acagaagcgc gatcggagag aaaatattca aagaaatcgt
    15961 aaagccgaac ccgaatgtcg tgatggtatt gagcgggcat tatcatagtg cgatgagaaa
    16021 aacagatgag ctggatgatg atggtgatgg gaaaccggat cgtcttgtgc atcaaatgct
    16081 ggccgattat cagggcgggc cagaaggcgg ccaagggtat ttgcgtttac ttcaatttga
    16141 ccaagccaat gacatggtgc acgtatcgac gtattctccg tatgtaaagg ataagaatta
    16201 ctatgatacg gatacctatg gcaacaagga tgaattttcc ctttcgcttg atttgaagcc
    16261 aagaatcaaa aaggtggaaa ccgattattt tgaatgtaat gtatatacca atgaagagct
    16321 cggaaaacgg gagcaagtga agagcgggga tacagctgaa ttccgctggg atcaccttga
    16381 atcgcagtct gtttattatt ggtacattat agtggaagac agttttaacg gcaaaacgaa
    16441 atcaccgatc tggaagttca agacgaaaaa agaaacatat cgtccggcac cagaccagtt
    16501 tgattttcgc catgtctcca atccttaaaa agggttggag gcttttttta tgaaagacga
    16561 tccatatagt ggacaaatgt ataacggtcg cctctgtata cggattttgc atgctcaaaa
    16621 gcagttccgt tctgcagata tgttgttcgt ttaattaata ggactggcgc tcccttttga
    16681 ataccaagaa tgttcgcttc ttccgtggtg gcagcgcttg gctcaagctc ctgttttgca
    16741 cgggaaatcg gtatgctgtt gtacctttca atatgatcat agatcgacga ctgaaaatgc
    16801 gattcgttca attcacccgc aagctcaaac ggaatatgtg aggactcaat cgccatcgga
    16861 atatcatttg ccagccgcac ccgagtgatt ttatggatag aggaggggtg cccgcagcct
    16921 aatatagccg cgagctcctc agttgaatca ataagctgat aatcaatgag cctgctgccc
    16981 ggtgtcatcc cgcggctttt catatcctcg gtaaagcttg tcagcccttg aagtgcttgt
    17041 tccatttttg gcttgctgac aaaggtgccc cgccccttca ggcgatagag caagccttca
    17101 ttaactaaat tagaaagcgc ctggcgaact gtcatccggc tgatcccgaa ttgttcggca
    17161 tattcgcgct cagaaggaag aggcatatcc ggctgcagtt ctccgctctt aatttgggtt
    17221 tttaattgct ccataatctg ataataaatc ggaataggcg attgtttatt gatattcata
    17281 agtcagcatg ttcctttcaa ggtcttaatg acgcggcttc acgatcaatc aaaaccgtga
    17341 catcggaatg caggtgcaaa gcagatgcgg gaaaatcttc gctgatgttt ccttctaaca
    17401 gcttacgaac agcttccgct ttgctttttc cagaaataag cagcagaatg cgcttgcttg
    17461 agagtatcgt ttgaattccc attgtcagtg cttttttcgg cacgctgtct atggagggaa
    17521 aatatctggc attcacttgg cgggtttgct catttaatgt cacaacatga gtttgcgact
    17581 tgaaagacgt tcccggttcg ttaaacccga tgtgtccgtt tcggccaatg ccgagaagct
    17641 gaatgtcagt gccgccgagg gactcgacta gctgttcata ccgtctgcat tcggcttcca
    17701 aatcgtctgc atttccattc ggaataaaat gccggctagg cttgctgtcg atatgctgaa
    17761 ataaacggtc attcatatag aagtgatagc tgttcgggtc attgcttgaa agtctggcgt
    17821 actcatccaa attaactgta gtgacgtttt gaaatgagag attctcagtt tggtgcaggc
    17881 ggatcagctc gcgatacgtg ccttccggtg tgctgcccgt cgctaaaccg agaacagcat
    17941 caggtttttc tttgattgta tctgccgtta ttctggctgc tatttggctt agctcttcat
    18001 acgtttgaca ttccattact ttcatatctg gtcagcctcc ttggatataa atgcaatgtt
    18061 tccgcggcaa atggtgagaa tcacctcgca atcactgctg acaatcacca aatctgcatc
    18121 ttttcccaca gtcacactgc cttttcggtc aaagatgccc agctgtttgg ccgcgtttgc
    18181 agaagttata ttagctatat ccgtccaaga gcaatttgta aactcacgca tatgccgtgc
    18241 gccttcgttc attttgagga tcgaaccggc aagggttccg tctgaaagaa gagctgttcg
    18301 tcctcttacc gtcacacttt ggccgccgaa ctcataaacg ccgtctttca ggccttttgc
    18361 cctcatggaa tcggtaatta aaatgagctt gctgctgcct ttcgccaaaa aagcaagctt
    18421 tgcagctaaa gggtgggagt gaatgccatc ggcgataagc tctgtcacaa acccatcatg
    18481 ggccagcgcc gttccgatca ctccgggctc tcggtgatga aacgggctca tcgcgttgta
    18541 cagatgggtc atatggctcg cccctgcttt tgcggcatcc gacagcaatg cggaatcggc
    18601 atccgtgtgc cccatggatg cgataatcga ttcgtctttc aaatggcgga tcagttcaaa
    18661 atgctgatcc tcctctggcg caagtgtaac aattttgatc agtccgcccg cctcctgctg
    18721 ccactttttg aaaagcgcaa catccgaagg cctaatccac tcttttggct gcgctccggc
    18781 tcttttagga gaaacaaagg ggccctctaa atgaataccg agcagttctg cacctaataa
    18841 agaggactct tcagccgctt tccactctct tgcgttcacc aaagcttgag aaatgttccc
    18901 atgctcttgg gtaatcgttg tggctaaaaa gctcgtcgtg ccttcttcgg gaagtcttga
    18961 cgacatgatg tcgagcgcag aaaagcttgc atccatcgta tctgcgccat agccgccgtg
    19021 gatatgaatg tcaatcatgc cgggaagcaa aacggaattc gcaggtgctt gaatctcttt
    19081 tgaatatggc tccttgggcc gttctgttga gactgtgctg atttttccgt cattgattcc
    19141 gacatagccg tttttgatta cttcattttc tgtcacgatc gcaatgtctt tgataagaag
    19201 actctctgcc atagatgatc cgcctttctg gaatttgaat acctttattt tactcttttt
    19261 cagactagtg atctagacca gctgaggtgt tttcccgcct tttataaaat ctgttttagg
    19321 ttcaagcctg aaagtgataa aatgaaagta ttgtgagcaa gctcggggca gcccggaaca
    19381 ggaggaacat atcgtggcaa aggttcgcac aaaagacgta atggaacagt tcaatttgga
    19441 attaatcagc ggagaagaag gaattaaccg cccgattaca atgagtgacc tatcaagacc
    19501 aggcattgaa attgccggat attttacata ttacccgaga gagcgcgtgc agcttctcgg
    19561 aaaaacagag ctttctttct ttgaacagct tccggaagag gataaaaaac agcgaatgga
    19621 ttctctgtgc actgatgtaa caccagctat tattctttct agagatatgc ccatcccgca
    19681 ggagctgatt gacgcctccg agaaaaatgg ggtgcctgtc ctcagatcgc cgctgaaaac
    19741 gacaagactg tcaagccgtt taaccaactt ccttgagagc aggctggcgc ctaccacagc
    19801 aattcatggc gttcttgtcg atatatatgg tgtcggcgtg ctgatcacag gaaaaagcgg
    19861 cgtcgggaaa agtgaaacag cgctggagct tgtgaaaaga gggcatcgcc ttgttgccga
    19921 tgattgcgtt gaaattcggc aggaggatca ggataccctt gtcggaaacg ctccggaatt
    19981 aattgagcat ctccttgaaa tcaggggcct tggcattatc aatgtgatga cgctatttgg
    20041 cgctggcgcg gtcagaagca ataaacgaat cacgattgtc atgaatcttg aactctggga
    20101 gcagggcaag caatacgacc ggctcggact tgaggaagag accatgaaaa tcattgatac
    20161 agaaattaca aaactgacga ttcccgtccg cccaggccga aatcttgcag tcatcatcga
    20221 agtggcagca atgaacttca gattgaagcg gatggggctg aatgccgctg aacaatttac
    20281 caacaagctg gcggacgtca ttgaagacgg tgaacaagaa gaataggagt tggcgttaaa
    20341 tgaatgaagc gatagaacca ctcaatccga tagcatttca gctggggccg ttagccgtcc
    20401 attggtacgg aatcattatc ggtctcggtg ctttgctcgg gctgtggatt gcgatgaggg
    20461 aaagtgaaaa acgggggctg cagaaagata cgtttattga tcttgtcctg tttgccattc
    20521 caattgcgat tatttgcgca cgaatttatt acgttgcctt tgaatgggac tattatgcgg
    20581 cacatccggg agaaatcatt aagatttgga agggcggcat tgccattcat ggcggattaa
    20641 tcggcgccat tttaacgggt tatgtgtttt cgagagtgaa aaacctttcg ttctggaagc
    20701 ttgcggatat tgccgcgccc agcattttgc tcggccaggc gatcggc
//
