LOCUS       NZ_JAPWIK010000037      6410 bp    DNA     linear   CON 14-FEB-2024
DEFINITION  Rhodococcus qingshengii strain IEGM 766
            NODE_37_length_6410_cov_278.771518, whole genome shotgun sequence.
ACCESSION   NZ_JAPWIK010000037
VERSION     NZ_JAPWIK010000037.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhodococcus qingshengii
  ORGANISM  Rhodococcus qingshengii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Krivoruchko,A.V. and Elkin,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2022) Lam, Institute of Ecology and Genetics of
            Microorganisms, Ural Branch of the Russian Academy of Sciences,
            Golev Str., 13, Perm, Perm Kray 614081, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: SPAdes v. 3.15.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 311x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/14/2024 02:58:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,449
            CDSs (total)                      :: 6,385
            Genes (coding)                    :: 6,320
            CDSs (with protein)               :: 6,320
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 17 of 65
            Pseudo Genes (incomplete)         :: 52 of 65
            Pseudo Genes (internal stop)      :: 6 of 65
            Pseudo Genes (multiple problems)  :: 9 of 65
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:17:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6410
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPWIK010000037.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     cand_cluster    1..6410
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6410
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:6397](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..6397)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(<1..6397)
                     /locus_tag="O4271_RS31835"
                     /old_locus_tag="O4271_31835"
     CDS             complement(<1..6397)
                     /NRPS_PKS="Domain: PCP (31-98). E-value: 6e-23. Score:
                     72.8. Matches aSDomain: nrpspksdomains_O4271_RS31835_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (115-420). E-value:
                     3.4e-111. Score: 363.0. Matches aSDomain:
                     nrpspksdomains_O4271_RS31835_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (596-987). E-value: 5.3e-93.
                     Score: 303.7. Matches aSDomain:
                     nrpspksdomains_O4271_RS31835_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (1094-1159). E-value: 3.7e-24.
                     Score: 76.7. Matches aSDomain:
                     nrpspksdomains_O4271_RS31835_PCP.2"
                     /NRPS_PKS="Domain: Epimerization (1176-1496). E-value:
                     1.2e-110. Score: 362.4. Matches aSDomain:
                     nrpspksdomains_O4271_RS31835_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1510-1667). E-value: 4.9e-43.
                     Score: 138.6. Matches aSDomain:
                     nrpspksdomains_O4271_RS31835_TIGR01720.1"
                     /NRPS_PKS="Domain: Condensation_DCL (1676-1977). E-value:
                     5.9e-100. Score: 326.2. Matches aSDomain:
                     nrpspksdomains_O4271_RS31835_Condensation_DCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="O4271_RS31835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_31835"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_269615150.1"
                     /sec_met_domain="Condensation (E-value: 4.4e-68, bitscore:
                     215.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.2e-105, bitscore:
                     337.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 6.4e-18, bitscore:
                     51.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVHGKLDRKALPDVTFEPQALQYVAPRTPVEETVAAIFDDLLGT
                     STVGVNDDFFALGGNSLSAARLVARVNSVLGERINIRDVFDASTVAALAELAQCRGGEG
                     AKPALTPRGMTTDIPLSPAQERMWFANQFAPESAAYNVACALRLEGAVNVSALHAAFVD
                     VVERHESLRTVFPLTDDGPRQVIMSTSQVVSDLAPILVQGEEELRERSRQIASDGFDVT
                     QQVPMRATLLRRDDQVHSHVLVIVIHHIAADGFSTVPLARDLMSAYSSRVNGKAPQWAP
                     LGIQYADYALWQRGWLGSESDSRSLISKQLGYWAENLAGAPEVLDIPTDRPRGDVRSLR
                     RGRIRFEFGPKVHAGVVALSRKHNATVFMVVHAALAVLLARLSDEDDVAVGTPIAGRGE
                     RELDDLVGMFVNTLVLRTAVDRGATFGDFLDQVRNVDLSAFIHADVPFERVVEIVNPSR
                     STAYSPLFQVMLEFQDIERPALELPDLQVTDVGVEIDTINFDLQLTVTRNAGAAGEYDG
                     LRAEFGYATDIFDEETVAGFADRLQRILERVITYPQSRIGDIEILDAYESAELVPVRGE
                     ISAAVQTLPDLLTAATDMATAQDPGAIALVFEEREVTYLELDVRSNRLARMLIESGVGP
                     ESVVALCLPRSIESVLAVWSIAKTGAAFLPVDPGHPAARIEHMLGDSGAGWGITSRAHT
                     AALSGLVPWLVLDDAGFEERLRAYSPSKVTDADRKAPIRIDNPVYLIYTSGSTGTPKGV
                     TVTHRGLSDFALQERETFAATTRSRTLHFASPSFDASVLELVMAVGAGATMVIVPPTVY
                     GGSELAALLERERVTHCFVTPAALTSVDPDGLTTVGCVVTGGEACPPELVARWAPGRKM
                     FNAYGPTEATVVSSISAALAPNDPVTIGTPTRGFSEVVLDSRLRAVPVGVAGELYLAGP
                     ALARGYHRRYPLTATQFVADPFGAPGTRIYRTGDRVRWTPDRRLEYLGRTDAQVKVRGF
                     RIELGEVEVALQTHPRVAQAVTAVWNGDGRDRLIGYVVPDRGASVDPRDVLAHAGKRLP
                     SYMVPAAVVVLDAVPLTVHGKLDRAALPTPDFSSAATPDRPPRTETENLLAALFAQVLG
                     LDAVGVHDSFFELGGDSIMSIQLVARARDAGVIISPRDVFDHKSVAALAESAHLEQDRP
                     DVLAELPGSAVGTVPLSPVAQWMLDRSSGGLRGGGVKRFSQALLLTAPADLTQDLLVRG
                     LQVLLDRHGMLRARLVESDRVMEVLPPGSISAEGLIHRVAVDSVDDEGFRALASGELEA
                     VADSLDPATADMVRVIWFDLADKPDVSTGRGRLLVVIHHLVVDGVSWRILVTDLASVTA
                     QLQAGVTPELPQIGTSFRRWAHGLVEHVGDRSVELDIWKTILDGDDPLIGSRPLDPTID
                     VDETVDTVTVDISPSATRNLLTTVPAAFHAGVNDGLVTAVALASIEWRRRRGMDTAAAL
                     ITLEGHGREEGAVPGADLARTVGWFTTLYPVRVELGAIDVDDALAGGADAGRALKAVKE
                     YVRAIPDHGIGFGMLRYLSGESDPLGRFRSPQISFNYLGRISSSDGTQDWLPVEGNRRL
                     GGTQKPSMPVASVIDINAAVEDHVDGPCLTASFTFPRGVLDRSDIAEFAELWRRALDGL
                     TAHASRPDAGGLTPSDIPLVSVDQQQIERWENEFPGLRDVWSVSPLQAGLMFHASLAAT
                     STDVYTAQLCVELEGAVDSERLRGAAAGLLANHPNLCAAFLYDVNGLPVQLIVDDAVIP
                     WTEVDLSDRGSATDADLERRLDEDRSAPFDLASPPLLRLTLLRTSPTRCVLVVTNHHIV
                     LDGWSIPLLVRELLMRYAADGLPVERAPADLPETPSYRAYLEWLMHRDMETSLAAWEDA
                     LEGVDEPTLLAPSTPIAEQLVPEEVAVDLPDSVRDGLGALVQRSGITMNTVIQAAWGVL
                     LSRMLSRDDVVFGVTVSGRSPELSGVENMMGLLINTLPVRVQLDPDETCTQLLARLQSE
                     QTALLDHHYVGLGAIQSVVGTGNLFDTLSVFESYPIDTSGMGGNTDIAGMRVTAVNARD
                     ATHYPLTVLSVAEPTLQVSLRYQPAAFDRAAVDTIAQRMSRILAAFAANADTPVGEVDV
                     FVAGERGLVVGGWDGVGVVVGG"
     source          1..6410
                     /collected_by="M.I. Rychkova"
                     /collection_date="2001"
                     /country="Russia: Udmurt Republic"
                     /db_xref="taxon:334542"
                     /isolation_source="oil-polluted soil, oil-extracting
                     enterprise"
                     /mol_type="genomic DNA"
                     /organism="Rhodococcus qingshengii"
                     /strain="IEGM 766"
                     /submitter_seqid="NODE_37_length_6410_cov_278.771518"
     region          1..6410
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     CDS_motif       complement(317..430)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0020"
                     /evalue="2.10E-13"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="O4271_RS31835"
                     /protein_end="2027"
                     /protein_start="1989"
                     /score="42.6"
                     /tool="antismash"
                     /translation="QSEQTALLDHHYVGLGAIQSVVGTGNLFDTLSVFESYP"
     aSDomain        complement(467..1369)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O4271_RS31835_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="5.90E-100"
                     /label="O4271_RS31835_Condensation_DCL.1"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1977"
                     /protein_start="1676"
                     /score="326.2"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVLDG"
                     /tool="antismash"
                     /translation="DVWSVSPLQAGLMFHASLAATSTDVYTAQLCVELEGAVDSERLRG
                     AAAGLLANHPNLCAAFLYDVNGLPVQLIVDDAVIPWTEVDLSDRGSATDADLERRLDED
                     RSAPFDLASPPLLRLTLLRTSPTRCVLVVTNHHIVLDGWSIPLLVRELLMRYAADGLPV
                     ERAPADLPETPSYRAYLEWLMHRDMETSLAAWEDALEGVDEPTLLAPSTPIAEQLVPEE
                     VAVDLPDSVRDGLGALVQRSGITMNTVIQAAWGVLLSRMLSRDDVVFGVTVSGRSPELS
                     GVENMMGLLINTLPVRVQLD"
     CDS_motif       complement(479..574)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0019"
                     /evalue="2.80E-18"
                     /label="C5_DCL_263-294"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1973"
                     /protein_start="1941"
                     /score="58.3"
                     /tool="antismash"
                     /translation="DVVFGVTVSGRSPELSGVENMMGLLINTLPVR"
     CDS_motif       complement(902..967)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0018"
                     /evalue="7.20E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1832"
                     /protein_start="1810"
                     /score="34.5"
                     /tool="antismash"
                     /translation="NHHIVLDGWSIPLLVRELLMRY"
     CDS_motif       complement(1181..1300)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0017"
                     /evalue="1.10E-10"
                     /label="C2_DCL_024-062"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1739"
                     /protein_start="1699"
                     /score="33.7"
                     /tool="antismash"
                     /translation="DVYTAQLCVELEGAVDSERLRGAAAGLLANHPNLCAAFLY"
     CDS_motif       complement(1322..1363)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0016"
                     /evalue="1.20E-02"
                     /label="C1_DCL_004-017"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1692"
                     /protein_start="1678"
                     /score="8.4"
                     /tool="antismash"
                     /translation="WSVSPLQAGLMFHA"
     aSDomain        complement(1397..1867)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O4271_RS31835_TIGR01720.1"
                     /evalue="4.90E-43"
                     /label="O4271_RS31835_TIGR01720.1"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1667"
                     /protein_start="1510"
                     /score="138.6"
                     /tool="antismash"
                     /translation="AGRALKAVKEYVRAIPDHGIGFGMLRYLSGESDPLGRFRSPQISF
                     NYLGRISSSDGTQDWLPVEGNRRLGGTQKPSMPVASVIDINAAVEDHVDGPCLTASFTF
                     PRGVLDRSDIAEFAELWRRALDGLTAHASRPDAGGLTPSDIPLVSVDQQQIER"
     aSModule        complement(1397..6052)
                     /complete
                     /domains="nrpspksdomains_O4271_RS31835_Condensation_LCL.1"
                     /domains="nrpspksdomains_O4271_RS31835_AMP-binding.1"
                     /domains="nrpspksdomains_O4271_RS31835_PCP.2"
                     /domains="nrpspksdomains_O4271_RS31835_Epimerization.1"
                     /domains="nrpspksdomains_O4271_RS31835_TIGR01720.1"
                     /locus_tags="O4271_RS31835"
                     /monomer_pairings="Leu -> D-Leu"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1784..1846)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0015"
                     /evalue="5.80E-10"
                     /label="NRPS-E6"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1538"
                     /protein_start="1517"
                     /score="31.3"
                     /tool="antismash"
                     /translation="VKEYVRAIPDHGIGFGMLRYL"
     aSDomain        complement(1910..2869)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O4271_RS31835_Epimerization.1"
                     /evalue="1.20E-110"
                     /label="O4271_RS31835_Epimerization.1"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1496"
                     /protein_start="1176"
                     /score="362.4"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="VGTVPLSPVAQWMLDRSSGGLRGGGVKRFSQALLLTAPADLTQDL
                     LVRGLQVLLDRHGMLRARLVESDRVMEVLPPGSISAEGLIHRVAVDSVDDEGFRALASG
                     ELEAVADSLDPATADMVRVIWFDLADKPDVSTGRGRLLVVIHHLVVDGVSWRILVTDLA
                     SVTAQLQAGVTPELPQIGTSFRRWAHGLVEHVGDRSVELDIWKTILDGDDPLIGSRPLD
                     PTIDVDETVDTVTVDISPSATRNLLTTVPAAFHAGVNDGLVTAVALASIEWRRRRGMDT
                     AAALITLEGHGREEGAVPGADLARTVGWFTTLYPVRVEL"
     CDS_motif       complement(1916..1966)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0014"
                     /evalue="1.10E-08"
                     /label="NRPS-E5"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1494"
                     /protein_start="1477"
                     /score="27.1"
                     /tool="antismash"
                     /translation="DLARTVGWFTTLYPVRV"
     CDS_motif       complement(2384..2449)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0013"
                     /evalue="2.40E-10"
                     /label="NRPS-E2"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1338"
                     /protein_start="1316"
                     /score="32.6"
                     /tool="antismash"
                     /translation="LLVVIHHLVVDGVSWRILVTDL"
     CDS_motif       complement(2681..2713)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0012"
                     /evalue="6.90E-03"
                     /label="Cy2"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1239"
                     /protein_start="1228"
                     /score="9.2"
                     /tool="antismash"
                     /translation="LLDRHGMLRAR"
     aSDomain        complement(2921..3115)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O4271_RS31835_PCP.2"
                     /evalue="3.70E-24"
                     /label="O4271_RS31835_PCP.2"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1159"
                     /protein_start="1094"
                     /score="76.7"
                     /tool="antismash"
                     /translation="ENLLAALFAQVLGLDAVGVHDSFFELGGDSIMSIQLVARARDAGV
                     IISPRDVFDHKSVAALAESA"
     CDS_motif       complement(3170..3196)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0011"
                     /evalue="6.40E-01"
                     /label="NRPS-A_a10"
                     /locus_tag="O4271_RS31835"
                     /protein_end="1076"
                     /protein_start="1067"
                     /score="3.5"
                     /tool="antismash"
                     /translation="GKLDRAALP"
     CDS_motif       complement(3407..3472)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0010"
                     /evalue="2.80E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="O4271_RS31835"
                     /protein_end="997"
                     /protein_start="975"
                     /score="35.4"
                     /tool="antismash"
                     /translation="YLGRTDAQVKVRGFRIELGEVE"
     aSDomain        complement(3437..4609)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O4271_RS31835_AMP-binding.1"
                     /evalue="5.30E-93"
                     /label="O4271_RS31835_AMP-binding.1"
                     /locus_tag="O4271_RS31835"
                     /protein_end="987"
                     /protein_start="596"
                     /score="303.7"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="AQDPGAIALVFEEREVTYLELDVRSNRLARMLIESGVGPESVVAL
                     CLPRSIESVLAVWSIAKTGAAFLPVDPGHPAARIEHMLGDSGAGWGITSRAHTAALSGL
                     VPWLVLDDAGFEERLRAYSPSKVTDADRKAPIRIDNPVYLIYTSGSTGTPKGVTVTHRG
                     LSDFALQERETFAATTRSRTLHFASPSFDASVLELVMAVGAGATMVIVPPTVYGGSELA
                     ALLERERVTHCFVTPAALTSVDPDGLTTVGCVVTGGEACPPELVARWAPGRKMFNAYGP
                     TEATVVSSISAALAPNDPVTIGTPTRGFSEVVLDSRLRAVPVGVAGELYLAGPALARGY
                     HRRYPLTATQFVADPFGAPGTRIYRTGDRVRWTPDRRLEYLGRTDAQVKVR"
     CDS_motif       complement(3557..3646)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0009"
                     /evalue="2.00E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="O4271_RS31835"
                     /protein_end="947"
                     /protein_start="917"
                     /score="39.3"
                     /tool="antismash"
                     /translation="PVGVAGELYLAGPALARGYHRRYPLTATQF"
     CDS_motif       complement(4127..4183)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0008"
                     /evalue="6.70E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="O4271_RS31835"
                     /protein_end="757"
                     /protein_start="738"
                     /score="27.9"
                     /tool="antismash"
                     /translation="YLIYTSGSTGTPKGVTVTH"
     CDS_motif       complement(4988..5101)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0007"
                     /evalue="6.10E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="O4271_RS31835"
                     /protein_end="470"
                     /protein_start="432"
                     /score="54.0"
                     /tool="antismash"
                     /translation="RNVDLSAFIHADVPFERVVEIVNPSRSTAYSPLFQVML"
     aSDomain        complement(5138..6052)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O4271_RS31835_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.40E-111"
                     /label="O4271_RS31835_Condensation_LCL.1"
                     /locus_tag="O4271_RS31835"
                     /protein_end="420"
                     /protein_start="115"
                     /score="363.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAADG"
                     /tool="antismash"
                     /translation="TDIPLSPAQERMWFANQFAPESAAYNVACALRLEGAVNVSALHAA
                     FVDVVERHESLRTVFPLTDDGPRQVIMSTSQVVSDLAPILVQGEEELRERSRQIASDGF
                     DVTQQVPMRATLLRRDDQVHSHVLVIVIHHIAADGFSTVPLARDLMSAYSSRVNGKAPQ
                     WAPLGIQYADYALWQRGWLGSESDSRSLISKQLGYWAENLAGAPEVLDIPTDRPRGDVR
                     SLRRGRIRFEFGPKVHAGVVALSRKHNATVFMVVHAALAVLLARLSDEDDVAVGTPIAG
                     RGERELDDLVGMFVNTLVLRTAVD"
     CDS_motif       complement(5150..5239)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0006"
                     /evalue="2.20E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="O4271_RS31835"
                     /protein_end="416"
                     /protein_start="386"
                     /score="48.9"
                     /tool="antismash"
                     /translation="DVAVGTPIAGRGERELDDLVGMFVNTLVLR"
     CDS_motif       complement(5507..5545)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0005"
                     /evalue="5.10E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="O4271_RS31835"
                     /protein_end="297"
                     /protein_start="284"
                     /score="18.9"
                     /tool="antismash"
                     /translation="QYADYALWQRGWL"
     CDS_motif       complement(5594..5659)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0004"
                     /evalue="3.80E-07"
                     /label="C3_LCL_132-143"
                     /locus_tag="O4271_RS31835"
                     /protein_end="268"
                     /protein_start="246"
                     /score="22.7"
                     /tool="antismash"
                     /translation="IHHIAADGFSTVPLARDLMSAY"
     CDS_motif       complement(5870..5986)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0003"
                     /evalue="3.40E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="O4271_RS31835"
                     /protein_end="176"
                     /protein_start="137"
                     /score="58.0"
                     /tool="antismash"
                     /translation="AAYNVACALRLEGAVNVSALHAAFVDVVERHESLRTVFP"
     CDS_motif       complement(6011..6043)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0002"
                     /evalue="1.40E+01"
                     /label="C1_DCL_004-017"
                     /locus_tag="O4271_RS31835"
                     /protein_end="129"
                     /protein_start="118"
                     /score="-0.2"
                     /tool="antismash"
                     /translation="PLSPAQERMWF"
     aSDomain        complement(6104..6304)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O4271_RS31835_PCP.1"
                     /evalue="6.00E-23"
                     /label="O4271_RS31835_PCP.1"
                     /locus_tag="O4271_RS31835"
                     /protein_end="98"
                     /protein_start="31"
                     /score="72.8"
                     /tool="antismash"
                     /translation="EETVAAIFDDLLGTSTVGVNDDFFALGGNSLSAARLVARVNSVLG
                     ERINIRDVFDASTVAALAELAQ"
     CDS_motif       complement(6359..6385)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O4271_RS31835_0001"
                     /evalue="1.70E-01"
                     /label="NRPS-A_a10"
                     /locus_tag="O4271_RS31835"
                     /protein_end="13"
                     /protein_start="4"
                     /score="5.1"
                     /tool="antismash"
                     /translation="GKLDRKALP"
ORIGIN
        1 aaccccccac cacaaccccc acaccatccc aaccacccac caccaaccca cgctcaccgg
       61 cgacaaacac gtcgacctca ccgaccggtg tatcggcgtt cgctgcgaag gcggcaagaa
      121 tccgcgacat tcgctgtgca atggtgtcga ccgcggcgcg gtcgaatgcc gccggctggt
      181 accgcaggct gacctgcagc gtcggttccg cgaccgacag cacggtgagc gggtaatgcg
      241 tggcgtcgcg ggcattcaca gcggtgacgc gcataccggc aatgtcggtg ttcccgccca
      301 tccccgaggt atcgatcgga tacgactcga agaccgacag tgtgtcgaac aaattgcctg
      361 tccctacgac agattgaatc gcgccgagtc cgacatagtg atgatcgagc agagctgtct
      421 gctcgctttg cagtcgagcg agcagctgcg tacacgtctc gtccggatcc agttgcactc
      481 ggacaggcaa cgtgttgatc agcagcccca tcatgttttc cacaccagac aactcgggtg
      541 agcggcccga aaccgtgacg ccgaagacta cgtcgtcacg ggaaagcatc cgagacagta
      601 ggacacccca cgccgcctgg atcaccgtgt tcatcgtgat cccgctccgt tggaccagcg
      661 caccgagtcc gtcgcgcacc gaatcgggta gatcgacggc aacctcttcg ggcaccagct
      721 gctccgcgat cggcgtcgaa ggcgccaaca acgtcggctc gtcgacgcct tccaaggcgt
      781 cctcccacgc cgcgagcgag gtctccatat cgcggtgcat caaccattcc agataggcgc
      841 ggtaggaggg agtctcggga agatcggccg gggccctctc gaccgggaga ccgtcggccg
      901 cgtatcgcat caacagttcg cgaaccagca acggtatcga ccagccgtcg agaacgatgt
      961 gatggttcgt caccaccaac acgcacctcg tcggcgaggt tcgcagcagc gtcagccgaa
     1021 gcagtggcgg cgaagcgagg tcgaagggtg cactgcgatc ctcgtcgagg cggcgctcca
     1081 aatctgcgtc ggttgccgat ccgcgatcac tcaggtcgac ctcggtccag ggaatcactg
     1141 catcgtcgac aatcaactgc accggtaggc cgttcacgtc gtagagaaac gctgcacaca
     1201 gattcgggtg gtttgcgagt agtccggcag cagcgccgcg caaacgctcg gaatcaaccg
     1261 ctccttcgag ctcgacgcac aactgcgctg tgtagacgtc cgtcgacgtg gccgccagtg
     1321 aggcatggaa catcagcccg gcctgaaggg gcgacaccga ccagacatcg cgcaatccgg
     1381 ggaattcgtt ctcccagcgt tcgatctgtt gttggtccac cgagaccagg ggaatgtccg
     1441 acggcgtcag accgcccgca tcaggtcgcg acgcgtgcgc ggtgagtccg tcgagcgcac
     1501 gacgccacag ttccgcgaac tccgcgatgt cagacctgtc gagcactccg cggggaaagg
     1561 tgaaagatgc tgtcaggcag ggcccgtcga catggtcctc aaccgccgcg ttgatgtcga
     1621 tcacggacgc cacaggcatg gacggcttct gggttccgcc gagccttcga ttgccctcca
     1681 ccggcagcca gtcctgtgtt ccgtcagagg agctgatccg gcccagatag ttgaaactga
     1741 tctgaggaga acggaatcgg ccgagtgggt cgctctcgcc agacaggtac cgcaacatcc
     1801 cgaatccgat tccatgatcg gggatcgcac gcacgtactc tttgacagct ttgagagccc
     1861 gcccggcgtc ggcgccacct gccagtgcgt cgtcgacgtc gattgcgcca agttccacgc
     1921 gcaccgggta gagcgtggtg aaccaaccaa cggtgcgagc gaggtctgca ccaggaaccg
     1981 ctccttcctc gcgcccgtgt ccttcgagcg tgatcagggc ggcagcggta tccataccgc
     2041 ggcgtcgtcg ccactcgatc gaggccaacg caacagcggt gacgagtccg tcgttcacgc
     2101 cggcgtggaa tgccgcagga accgtggtga gaaggttcct ggtcgccgaa ggcgagatgt
     2161 ccactgtaac ggtgtcgacc gtctcgtcga cgtcgatcgt gggatcgagt gggcgcgaac
     2221 cgatcaacgg gtcatcgccg tccaggatcg ttttccatat gtccagttcg acgctccggt
     2281 cgcccacgtg ctcgacaagc ccatgcgccc atcgccggaa cgacgttccg atctgtggca
     2341 gttcgggcgt gacaccggcc tggagttgtg ctgtcaccga cgccaggtcg gtgaccagaa
     2401 tgcgccacga gacgccgtcg acaaccaggt gatgaatcac cacaagtagt cggccccgcc
     2461 cggtcgacac gtcaggtttg tcggccaggt cgaaccagat cacgcgcacc atgtcagccg
     2521 tggcgggatc caagctgtcg gccacggcct cgagttctcc cgacgccaag gccctgaaac
     2581 cttcgtcgtc caccgaatcg acagctacgc ggtggatcag tccttcggcc gagatcgagc
     2641 cgggtggtaa gacttccatg acccggtcac tctcaaccaa gcgagctcga agcattccgt
     2701 ggcggtccag caacacctgg agaccacgga caaggaggtc ctgggtgaga tcagcgggag
     2761 ctgtcagcaa gagcgcctgg gagaaccgtt tcactccgcc gccacgcagc ccgccactgc
     2821 tgcggtcgag catccactgc gccaccgggc tcagtggaac agttccgaca gcggatcccg
     2881 gtagctccgc gagtacatca ggcctgtcct gctcaaggtg cgccgactcg gcaagcgcgg
     2941 cgacggattt gtggtcgaaa acatctcggg gtgagatgat cacacccgca tcgcgagcgc
     3001 gggccaccaa ttggatggac atgatgctgt cgccaccgag ctcgaagaac gaatcatgca
     3061 ctcctaccgc gtcgagaccg agaacctgcg cgaacagagc ggcaagaaga ttctcggttt
     3121 ccgttcgcgg tggccggtcg ggtgtggcgg cggacgagaa atcgggtgtg ggaagcgctg
     3181 cgcggtcgag ttttccgtga accgtcagcg gaacggcgtc gagcaccacc accgccgccg
     3241 gcaccatgta cgacggcagg cgcttgcctg catgggcgag gacgtctcgc ggatcgacac
     3301 tcgctccgcg atcgggaacc acgtagccga tcaaccgatc cctgccgtcg ccgttccaga
     3361 cggccgtcac cgcctgggcc acgcgcgggt gcgtctgcag tgccacctcg acttccccga
     3421 gttcgatgcg gaacccacgg actttgactt gggcatcggt gcgcccgaga tactccagcc
     3481 gtcggtcggg cgtccaccgc acgcgatcgc ccgtgcgata gatccgcgtt cccggcgcac
     3541 cgaagggatc tgcgacaaac tgcgtggcgg tcaacgggta tcgccggtga tagccgcgag
     3601 cgagagccgg cccggcaaga tacaactccc ccgcgacacc taccggtacc gctcgcagac
     3661 gcgagtccag cactacctcg ctgaatccgc gagtaggtgt gccgatagtg accgggtcgt
     3721 tcggagccag tgccgcactg atgctcgaca cgacagttgc ctcggtagga ccgtaggcgt
     3781 tgaacatctt tctgcccggc gcccagcggg ccaccaattc cggcggacac gcttccccac
     3841 ctgtcacgac acaaccgaca gtggtgagac cgtctgggtc gacggacgtt aaggccgccg
     3901 gagtcacaaa acaatgggtg acgcgctcgc gctccagtag cgcggccagt tccgacccgc
     3961 cgtagacggt cggtggcacg atcaccatcg tggctccagc accaacggcc atcacgagct
     4021 cgagaaccga cgcgtcgaaa ctcggcgaag cgaagtgcag agttctggat cgggtcgtgg
     4081 cggcgaaggt ctcacgctcc tggagcgcga agtcgctcaa cccgcgatgt gtgaccgtca
     4141 cgcccttggg tgttcctgtc gaccccgacg tgtagatcag atagaccgga ttgtcgatgc
     4201 gaatcggtgc cttccgatcc gcgtcggtga ctttcgatgg cgagtacgcc cgcagccgtt
     4261 cctcgaaacc ggcatcgtcg aggaccagcc acggcacgag gccggacaat gccgcggtgt
     4321 gagctcgaga cgttatcccc caccctgcac cggagtcgcc cagcatgtgc tcgatacgag
     4381 cagcaggatg tcccgggtcc acgggaagga atgccgcacc tgtcttggca atggaccaca
     4441 ccgccagaac ggactcgatg gagcgtggga gacacaacgc caccaccgat tcgggaccca
     4501 ctccggattc gatcagcatg cgggccaagc ggttcgaccg cacgtcgagt tcgagatagg
     4561 tcacttcacg ttcctcgaac accagcgcga tggcgccggg atcctgggcg gtcgccatat
     4621 ctgttgctgc agtcagcaaa tccggaaggg tctgaacagc ggcggaaatc tcacctcgca
     4681 cgggtaccag ttcggctgac tcataggcgt cgaggatctc gatatctccg atccgtgatt
     4741 gcggatacgt gataacgcgc tcgagaatcc gttgcaggcg gtcggcgaag cccgctaccg
     4801 tttcctcgtc gaaaatatct gtggcatatc cgaattcggc gcgaagaccg tcgtattcgc
     4861 cggctgcgcc ggcgttccgc gtcaccgtca actgaagatc gaagttgatg gtgtcgatct
     4921 cgacgcccac gtcggtcacc tgcaggtcgg ggagttcgag tgcaggccgt tcgatgtctt
     4981 ggaactccaa catgacttga aacaggggcg agtacgccgt cgaacgtgaa ggattcacga
     5041 tctcgacgac tctttcgaac ggcacgtccg cgtgaatgaa ggcgctcaga tctacgtttc
     5101 ggacctggtc gaggaagtct ccgaaagttg cgccgcggtc cactgcagtg cgcaagacca
     5161 acgtgttgac gaacatgccc accaggtcgt cgagttcgcg ctccccacgc ccggcgatgg
     5221 gagttccgac ggctacgtcg tcctcatcac tgagcctcgc cagcagcact gccaaggctg
     5281 cgtgcacaac catgaagacc gtggcgttgt gtttgcgcga cagggcaacc acaccggcat
     5341 gcaccttcgg accgaactcg aacctgatcc gtccgcgtcg aagcgaacgc acatcaccgc
     5401 gcggtcgatc ggtcggaatg tcgagaacct caggtgcgcc cgcaagattc tcggcccaat
     5461 agccgagctg tttcgagatc agagaccgag agtccgattc cgagcccaac caaccacgtt
     5521 gccacaacgc gtagtcggca tactggatcc cgagcggcgc ccactgcggt gccttaccgt
     5581 tcacacggga ggagtatgcg ctcatgagat ctcgggccag cggtaccgtc gagaatccgt
     5641 cggcggcgat gtggtgaatc acgatcacca ggacatgcga gtggacctgg tcatctcgcc
     5701 gcaacaaggt ggcgcgcatc gggacctgct gcgtgacgtc gaaaccatcc gaagcgatct
     5761 gccggctacg ctcgcgcagc tcttcctctc cctgcacaag tatcggtgca agatccgata
     5821 ccacctgtga ggtggacatg atcacttgac gcgggccgtc gtcagtgaga ggaaaaacag
     5881 tgcgcaagga ctcgtgccgc tcgacgacgt cgacaaatgc agcgtgaagc gcactcacgt
     5941 tcactgcacc ttcgagcctc aacgcgcacg cgacgttgta ggcggcggac tcgggggcga
     6001 actgattcgc aaaccacatt cgctcctgcg ccggtgaaag tgggatatct gtcgtcatac
     6061 cccgcggcgt caacgccggc ttggccccct ctcctccgcg acactgcgcc aattcggcca
     6121 gcgctgcaac ggtagaagca tcgaagacgt cacgaatgtt gatccgttcg ccgagaacag
     6181 aattcactcg cgcaaccaac ctggccgcac tgagagagtt cccacccagg gcaaaaaagt
     6241 cgtcgttcac gcccaccgtc gacgttccca gcagatcatc gaagattgcg gcaaccgtct
     6301 cctcgaccgg cgttcgtggc gcaacatact gcagcgcttg aggttcgaag gtgacgtccg
     6361 ggagggcttt acggtccaac ttcccatgca ccgtcaccgg aaactccccc
//
