LOCUS NZ_JAPWIK010000003 11931 bp DNA linear CON 14-FEB-2024 DEFINITION Rhodococcus qingshengii strain IEGM 766 NODE_3_length_653427_cov_321.115482, whole genome shotgun sequence. ACCESSION NZ_JAPWIK010000003 VERSION NZ_JAPWIK010000003.1 KEYWORDS WGS; RefSeq. SOURCE Rhodococcus qingshengii ORGANISM Rhodococcus qingshengii Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group. REFERENCE 1 (bases 1 to 653427) AUTHORS Krivoruchko,A.V. and Elkin,A. TITLE Direct Submission JOURNAL Submitted (15-DEC-2022) Lam, Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Russian Academy of Sciences, Golev Str., 13, Perm, Perm Kray 614081, Russia COMMENT ##Genome-Assembly-Data-START## Assembly Date :: 2022 Assembly Method :: SPAdes v. 3.15.4 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 311x Sequencing Technology :: Illumina NovaSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Date :: 02/14/2024 02:58:48 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.6 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 6,449 CDSs (total) :: 6,385 Genes (coding) :: 6,320 CDSs (with protein) :: 6,320 Genes (RNA) :: 64 rRNAs :: 1, 1, 5 (5S, 16S, 23S) complete rRNAs :: 1, 1 (5S, 16S) partial rRNAs :: 5 (23S) tRNAs :: 54 ncRNAs :: 3 Pseudo Genes (total) :: 65 CDSs (without protein) :: 65 Pseudo Genes (ambiguous residues) :: 0 of 65 Pseudo Genes (frameshifted) :: 17 of 65 Pseudo Genes (incomplete) :: 52 of 65 Pseudo Genes (internal stop) :: 6 of 65 Pseudo Genes (multiple problems) :: 9 of 65 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-13 05:17:55 NOTE :: This is a single region extracted from a larger record! Orig. start :: 19879 Orig. end :: 31810 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to JAPWIK010000003.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..11931 /candidate_cluster_numbers="1" /contig_edge="False" /product="RiPP-like" /region_number="1" /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /tool="antismash" cand_cluster 1..11931 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /kind="single" /product="RiPP-like" /protoclusters="1" /tool="antismash" protocluster 1..11931 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="False" /core_location="[5000:6931]" /cutoff="20000" /detection_rule="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /neighbourhood="5000" /product="RiPP-like" /protocluster_number="1" /tool="antismash" proto_core 5001..6931 /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /neighbourhood="5000" /product="RiPP-like" /protocluster_number="1" misc_feature complement(124..135) /note="TFBS match to CcpA, Catabolite control protein A, confidence: weak, score: 16.57" /tool="antismash" misc_feature 467..482 /note="TFBS match to LexA_variant_1, Repressor of DNA damage response, confidence: weak, score: 20.68" /tool="antismash" gene complement(975..2198) /locus_tag="O4271_RS11870" /old_locus_tag="O4271_11865" CDS complement(975..2198) /EC_number="2.3.1.-" /GO_process="GO:0006096 - glycolytic process [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005113320.1" /locus_tag="O4271_RS11870" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11865" /product="dihydrolipoamide acetyltransferase family protein" /protein_id="WP_269611404.1" /transl_table=11 /translation="MSNQVFMLPDLGEGLAEADIAEWHVKVGDVVTIDQIVVEVETAKA AVDVPIPFAGTVIELHGKDGDTLKVGTPLITVSGGESVDAVVSANHERYREEERAGSGN VLIGYGTSEDAPLRRRRAAPSVRVISPIVRKLASDNSIDLTTISGSGAGGVITRADVEA GSNTGTELETSHAADQRIPIKGLRKVVADKLSTSRREIPDATTWVDVDATELLAARAEI NKSLPDSDKISLMALLARLTIAALAQYPELNSSVDTARGEIVRHARTHLGIAAQTPKGL MVPVIRNADALSTVELAQALRVTTDLARDGKLEPARLTGGTFTLNNYGVFGVDGSAPII NHPEAAILGIGRIIDKPWVVNGELTVRKVTQISLSFDHRVCDGGEAGGFLRLFGDYIEN PIRVLGRL" gene complement(2195..3199) /locus_tag="O4271_RS11875" /old_locus_tag="O4271_11870" CDS complement(2195..3199) /EC_number="1.2.4.-" /GO_function="GO:0016491 - oxidoreductase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (smcogs) SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005254623.1" /locus_tag="O4271_RS11875" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11870" /product="alpha-ketoacid dehydrogenase subunit beta" /protein_id="WP_269611405.1" /transl_table=11 /translation="MPMLTMAQALNTSLRDSLAADDNVVVFGEDVGTLGGVFRVTDGLT RDFGDDRCFDTPLAESGIIGFAIGMAMAGFRPVVEMQFDAFAYPAFEQIASHVAKIRNR TKGALSIPIVIRVPFAGGIGGVEHHCDSSEGYYAHTPGLKVVAPSTVEDAYSLLRSAIE DPDPVIFLEPKKLYFARADVELTAREPIGRAVVRRPGRDATLIAYGPSVDVALRSAEAA AAEGRDIEVIDIRSIVPFDDETVTASVRKTGRCIVIQEAQGFAGVGAEIAARVQERCFH HLHAPVLRVSGFDIPYPAPKLERHHLPSIDRVLDAVDRLQWNDAPDTRWAVTA" gene complement(3202..4329) /gene="pdhA" /locus_tag="O4271_RS11880" /old_locus_tag="O4271_11875" CDS complement(3202..4329) /GO_component="GO:0045250 - cytosolic pyruvate dehydrogenase complex [Evidence IEA]" /GO_function="GO:0004739 - pyruvate dehydrogenase (acetyl-transferring) activity [Evidence IEA]" /GO_process="GO:0006086 - acetyl-CoA biosynthetic process from pyruvate [Evidence IEA]" /codon_start=1 /gene="pdhA" /gene_functions="biosynthetic-additional (rule-based-clusters) DXP_synthase_N" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019746167.1" /locus_tag="O4271_RS11880" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11875" /product="pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha" /protein_id="WP_217018155.1" /sec_met_domain="DXP_synthase_N (E-value: 1.7e-06, bitscore: 22.6, seeds: 174, tool: rule-based-clusters)" /transl_table=11 /translation="MTLVEPLGELRYQKFLPADSAVQYLDPAGELTRSEARYAKPSDDR LIAMYRKMFLGRRFDQQATALTKQGRLAVYPSSRGQEACQIAAAMSLEPSDWLFPTYRD SMALAARGVDPVQILSMLAGDWHCGYDPVALRSAPQCTPLATQLLHAAGVAYGESRRGL NTVALALCGDGATSEGDFHEALNFAAVFKAPVIFLVQNNGFAISVPLSRQSAAPTLAHK GVGYGIGSEQVDGNDPVAMMAVMDEAARFVRSGNGPVIVEAHTYRIDAHTNADDATRYR DSAEVEAWLGRDPLPRLEKYLRAHDLIDDAFVESLTAEAETAAATLRAGMNVDRPHDPL DLFRYVFAEQTPQLREQQAQLETELAAEAADTGGH" gene 4484..4954 /locus_tag="O4271_RS11885" /old_locus_tag="O4271_11880" CDS 4484..4954 /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1120: AsnC family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007729068.1" /locus_tag="O4271_RS11885" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11880" /product="Lrp/AsnC family transcriptional regulator" /protein_id="WP_042447206.1" /transl_table=11 /translation="MATEPHALDAIDRRIIAELVADSRISMRALAEKAHISRAHAYVRI ERLEDAGIITGFTTRIAHEKAGLGSSAYVALSIRQHSWRGLAASLKALPFVEHFSLLGG DFDVLVLVRAPDNQELRHTVLEELQGLDGVLSTKTWLIFEEGNGPGPNWLPA" gene 5001..5825 /locus_tag="O4271_RS11890" /old_locus_tag="O4271_11885" CDS 5001..5825 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) RiPP-like: DUF692" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007729070.1" /locus_tag="O4271_RS11890" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11885" /product="DUF692 domain-containing protein" /protein_id="WP_269611408.1" /sec_met_domain="DUF692 (E-value: 2.3e-71, bitscore: 235.3, seeds: 7, tool: rule-based-clusters)" /transl_table=11 /translation="MTLYGLGIGWRPEVSGIIAQLDGLAFCEVIAESVGNEIPPHLGSL GVPVIPHGISLSLGGAEAVDQARIDHLAHAAQLLGAPLASEHIAFVRAGGIEAGHLLPL PRTYEAVDALVRNITRTQDGLPVPFAVENIAALFDWPDDELTEGEFLAEIVERTGALLV LDIANVYANALNRGRDPWTELSRLPLERIAYCHIAGGSLGDGKYHDTHTDPVPEAVLEL LAQFTAAGHHPAIMLERDGNYPPALELLDELDAIADAAGLDRITAGTDWSRT" gene 5822..6148 /locus_tag="O4271_RS11895" /old_locus_tag="O4271_11890" CDS 5822..6148 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007729071.1" /locus_tag="O4271_RS11895" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11890" /product="hypothetical protein" /protein_id="WP_030537467.1" /transl_table=11 /translation="MSTLGERQEALIRALVAGGELPEGFDKDEAAVVSAALLRKRAGEV AHHLPVVRHTLGDRYLQLFTAWAGGRPKTSSRSDAQAFVAHLQDIGELPRPPWYQRFRK LSRK" gene complement(6137..6931) /locus_tag="O4271_RS11900" /old_locus_tag="O4271_11895" CDS complement(6137..6931) /GO_component="GO:0140737 - encapsulin nanocompartment [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) RiPP-like: Linocin_M18" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003942478.1" /locus_tag="O4271_RS11900" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11895" /product="family 1 encapsulin nanocompartment shell protein" /protein_id="WP_003942478.1" /sec_met_domain="Linocin_M18 (E-value: 2.2e-98, bitscore: 323.6, seeds: 61, tool: rule-based-clusters)" /transl_table=11 /translation="MTNLHRDLAPISAAAWAEIEEEASRTFKRHVAGRRVVDVEGPSGD DLAAIPLGHQVPINPLADGVIAHARQSQPIIELRVPFTVSRQAIDDVERGAKDSDWQPV KDAAKQIAFAEDRAIFEGYPAASITGVRASGSNPELKLPIDAKDYPEAISQAITSLRLA GVNGPYSLLLNADAFTAINETSDHGYPIREHLRRVLDGEIIWAPAIDGAFLLSTRGGDY ELHLGQDLSIGYLSHDANSVELYFQESMTFLMYTSEAVVSLA" gene complement(6928..7986) /locus_tag="O4271_RS11905" /old_locus_tag="O4271_11900" CDS complement(6928..7986) /GO_function="GO:0004601 - peroxidase activity [Evidence IEA]" /GO_function="GO:0020037 - heme binding [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003942561.1" /locus_tag="O4271_RS11905" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11900" /product="Dyp-type peroxidase" /protein_id="WP_042447536.1" /transl_table=11 /translation="MAETSEIDEAEVALPAIPQPLLTPLTEAAIFLVFTIDEGGEQAVH DVLADISGLQRSIGFRVPAGGLAAVVGIGSDAWDRLFDGPRPAELHPFVELTGDKHHAP RTPGDLLFHIRARQMDLCFEFATVVTNRLAGAASVIDEVHGFKYFEQRDLMGFVDGTEN PSGQAAYVAVTVGDEDPDFAGSSYVIVQKYLHDMSEWNSLPVEEQENVIGRSKLEDLEM DDDTKPANSHTALTVIEDESGEQIQILRDNMPFGHVGSAEMGTYFIGYSASPTVTEQML TNMFIGNPVGNYDRILDFSTAVTGINFFVPTADFLDDPPDLPTRLVPEATFTAPISDGS LGIGSLKRSAQQ" gene complement(8055..9011) /locus_tag="O4271_RS11910" /old_locus_tag="O4271_11905" CDS complement(8055..9011) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019746173.1" /locus_tag="O4271_RS11910" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11905" /product="SMP-30/gluconolactonase/LRE family protein" /protein_id="WP_007729077.1" /transl_table=11 /translation="MRTRIFDRGIRVGITGALVAGSLLALGVSGAAAAPPCPGSSPAIL SAQIPGAALEGVTVDGQGRLYTTDLVSGRIFRVDGPGQAAVPIATVPGGAGALAWSPDG SLLVGTGANAGVLIGDLTRAASVARVDVNSGAVTPIAGGLSAANGLAVAHDGTIYATND FGSLIGKVSPNGSVDPAWATFPSANGAALSADNRWLYVSRTFANPGVSRISVDNPGYSE SLVDLGGFETFSAPDGLTLDSRDRAIVPTDISGEVWRIDGPGQYCSLTSGLQASSVLTY GRGNSGFAAGHLYRAGFDGKIFEIPAGFDAGAQAATP" gene complement(9045..10001) /locus_tag="O4271_RS11915" /old_locus_tag="O4271_11910" CDS complement(9045..10001) /GO_function="GO:0016787 - hydrolase activity [Evidence IEA]" /NRPS_PKS="Domain: Abhydrolase_1 (22-303). E-value: 2.2e-34. Score: 111.1. Matches aSDomain: nrpspksdomains_O4271_RS11915_Abhydrolase_1.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) PF00561" /gene_functions="biosynthetic-additional (rule-based-clusters) Abhydrolase_6" /gene_functions="biosynthetic-additional (smcogs) SMCOG1036: alpha/beta hydrolase fold protein" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003942554.1" /locus_tag="O4271_RS11915" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11910" /product="alpha/beta hydrolase" /protein_id="WP_083058112.1" /sec_met_domain="PF00561 (E-value: 4.9e-28, bitscore: 93.6, seeds: 48, tool: rule-based-clusters)" /sec_met_domain="Abhydrolase_6 (E-value: 2e-17, bitscore: 59.8, seeds: 455, tool: rule-based-clusters)" /transl_table=11 /translation="MTVKTIDVDGFCWQIDDSGSGTAVVMCHGFPGLGYSYRHQSAALA EAGFRSIALDMPGYGGTTRPDAIEDYTNDAVANRLIDLLDALGIDKAVFVGHDFGAPVA WTTALRHPDRVAGLVSLAVPYAPDRFPAKPSAIYAALARKHFLHIHYFQEPGVAERELD ARPREFLQRLFHALSGAYRYLDVWKNPSEGNGYLDVLPEAPALPWSWLTEDDLDVYVKA FTATGFTGGLNWYRAYDANWERSADLDGAHIEVPTLFVAGAEDPVIAMSGPKALDRMKD TVPDLRGVHLLGGAGHFVQQEHAAEVNELLISFLRSL" aSDomain complement(9093..9935) /aSDomain="Abhydrolase_1" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_O4271_RS11915_Abhydrolase_1.1" /evalue="2.20E-34" /label="O4271_RS11915_Abhydrolase_1.1" /locus_tag="O4271_RS11915" /protein_end="303" /protein_start="22" /score="111.1" /tool="antismash" /translation="AVVMCHGFPGLGYSYRHQSAALAEAGFRSIALDMPGYGGTTRPDA IEDYTNDAVANRLIDLLDALGIDKAVFVGHDFGAPVAWTTALRHPDRVAGLVSLAVPYA PDRFPAKPSAIYAALARKHFLHIHYFQEPGVAERELDARPREFLQRLFHALSGAYRYLD VWKNPSEGNGYLDVLPEAPALPWSWLTEDDLDVYVKAFTATGFTGGLNWYRAYDANWER SADLDGAHIEVPTLFVAGAEDPVIAMSGPKALDRMKDTVPDLRGVHLLGGAGHFVQQEH " misc_feature 9991..10006 /note="TFBS match to RutR, Regulator of the pyrimidine and purine metabolism, confidence: medium, score: 22.22" /tool="antismash" gene complement(10003..10377) /locus_tag="O4271_RS11920" /old_locus_tag="O4271_11915" CDS complement(10003..10377) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005262400.1" /locus_tag="O4271_RS11920" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11915" /product="nuclear transport factor 2 family protein" /protein_id="WP_003942502.1" /transl_table=11 /translation="MAPSAEVIRKTVDAYIETVATGTAAQVVALYAEGATVEDPVGTDV RTTRESILEFYSVIENFDQEAHLQTLRIAGNEAAFHFSLITKLGEQTLTIAPIDVMTFD DDGKILSMRAFWNQEDMITA" misc_feature 10404..10418 /note="TFBS match to SypG, Regulator of biofilm formation and host colonization, confidence: weak, score: 19.5" /tool="antismash" gene complement(10443..11123) /locus_tag="O4271_RS11925" /old_locus_tag="O4271_11920" CDS complement(10443..11123) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003942449.1" /locus_tag="O4271_RS11925" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="O4271_11920" /product="hypothetical protein" /protein_id="WP_269611415.1" /transl_table=11 /translation="MTFADLDGDELWSYLTERSGLPGPRVNLGLMLEFARGADDDDILL AAESEDEYIRCCGIVGLGFILVRSRDKAVLNSLTEATTSASWRAREAAAMAVQAIGDTD PQLLRAIIEQWSRSAHPLTLRAAAAGICEPRLLKDKTNAALAVQVCRDATKWIVSQPAD SRRDADTRTLRQALGYCWSVAVAADPENALPAFVSLGASDDTDVVWIVRENRKKARLRK VLES" ORIGIN 1 gattccttgt tggaaacctc ggtgcgccaa cgcaagaccg ggcacgatgt cgccgaccgc 61 gtacacaccg tccacggacg tacggaggcg ttcgtcggtg acgacaaatc ccttgtcgag 121 gctgattccg ttttcttcga aaccgtcacc gtccgtgcga ggtccgcggc cgacggcgac 181 caacagtagg tcggtgtcga gtttcgttcc gtcgcttagt tcgatgcgga cggaatcggc 241 tgcttctttc gcggaatcga cctttgtgtc tgtcttacag acgattccac gtttccggta 301 tgcccgctcc agctgtttcg acgaccacgg atcctcggcg gcgatcagtc gggggagtgc 361 ctcgacgatc gttacttcgg cgccgaacga gcgccagagg cttgcgaact cgacgccgat 421 gacaccgcca ccgagtaccg ttgcgctggt gggcacccgg tcgagttcga gtgcctggtc 481 gctggtgacg atccgtcggc cgagttcgat gccggggagt tcccgcggat acgagccggt 541 ggccagaacg agtgatgttc cggtgtagcg ggttccgtcg acgtcgatgc ttcgcccacc 601 gacatacgtc ccgtatccgt tgacgatggt gatcttgtgt tgcgccaaca agccttgcag 661 accgctgtac agccgctcga ctgtcccgtt cttgtagtcg tgcacctgcg cgacgtcgat 721 gccggcaaaa gatgcccgga caccgaattg ctcactggtg cgtgccgaat cggccacctc 781 ggcggaatgc agcagtgcct tggtcgggat gcatccgcgg tgcaggcacg ttccgccgac 841 cttgtccgct tcgatcagcg tgaccgacaa tccgagttga gcggcgcgga tggcgcaggc 901 atagccgccc gatcctccac cgaggatcac gacgtcgctg tggggttctg cagttgtcat 961 cggagccttg gtcatcagag ccttcccagg acgcggatcg ggttctcgat gtagtcaccg 1021 aacagcctca ggaatccgcc ggcttcgccc ccgtcacaga cgcggtggtc gaagctcaac 1081 gagatctgag tgaccttacg aacggtcaac tcgccgttca cgacccacgg cttgtcgatg 1141 atgcgtccga tccccagaat tgccgcctcg gggtgattga tgatcggagc cgatccgtcg 1201 accccgaata ccccgtagtt gttgagcgtg aacgtgccgc cggtcaggcg ggcgggttcg 1261 agcttgccgt cgcgggcaag gtcggtggtc acccgaagtg cttgtgcgag ttcgacggtg 1321 ctcagggcgt ccgcatttcg aatgaccggt accatcaatc ccttcggtgt ctgcgcggcg 1381 atccccaggt gggtgcgggc gtgtcggacg atctcgcctc gagcagtatc gaccgacgag 1441 ttcagttcgg ggtactgcgc caacgcagcg atcgtcagcc gagccaggag cgccatcagg 1501 ctgatcttgt cggaatcggg cagtgacttg ttgatctcgg ctcgcgccgc caagagttcg 1561 gtggcgtcga cgtcaaccca ggttgttgcg tccgggatct cacggcggct ggtggacagc 1621 ttgtcggcaa ccaccttgcg tagtcccttg atcgggatcc gctggtcggc cgcgtgtgac 1681 gtttccaatt ccgtgccggt gttcgacccg gcctcgacgt cggcgcgcgt gatgactccg 1741 ccggcgcccg aaccggaaat cgtggttagg tcgatactgt tgtcagaggc cagtttacga 1801 acgatcggcg agatcacgcg taccgacgga gccgctctgc ggcggcgcag aggtgcatcc 1861 tcgctggtgc cgtagccgat caggacgttg cccgaaccgg cgcgttcctc ttcgcggtag 1921 cgctcgtggt tggccgaaac gaccgcgtcg acactctcgc caccactcac agtgatcagc 1981 ggtgtgccca ccttcagggt gtctccgtct ttgccgtgga gttcgatcac ggttccggcg 2041 aacgggatgg gcacatccac agcagctttc gcggtctcga cctcgacgac gatctgatcg 2101 atggtgacca cgtcgccgac cttgacgtgc cactccgcga tgtcggcctc ggccagacct 2161 tcacccagat cggggagcat gaatacctga ttgctcatgc cgtcaccgcc caacgggtgt 2221 ccggggcgtc gttccactgc agacggtcga cggcgtccag gacccggtcg atgctgggca 2281 ggtggtgacg ctcgagcttg ggagcggggt aggggatgtc gaagccgctg acccggagca 2341 ccggggcatg gagatggtgg aaacaccgtt cctgcacgcg ggcagcaatt tctgcaccca 2401 caccggcaaa accctgcgct tcctggatga cgatgcagcg tccggtcttt cgcaccgatg 2461 cggtgaccgt ctcgtcgtcg aacggcacta tcgagcggat gtcgatcacc tcgatgtccc 2521 ggccctcggc tgcagcggcc tcggcggacc tcagtgcaac gtccacggac ggaccgtagg 2581 cgatgagcgt cgcgtcacga ccggggcgac ggacgacggc gcgaccgatc ggctcacgtg 2641 cggtcagttc gacatctgct cgcgcgaagt acagcttctt gggctcgagg aagatcacgg 2701 ggtcggggtc ctcgattgcc gaacgtagca gcgagtacgc atcttccacc gtggacggcg 2761 ccacaacctt cagtccgggt gtgtgcgcgt agtagccctc actggaatca cagtgatgct 2821 ccaccccgcc gataccgccg gcaaaaggaa cacgaatgac tatcggaatc gacagcgcgc 2881 ccttggtgcg gttgcggatc ttcgccacat gggaggcgat ctgctcgaac gccggatagg 2941 caaacgcgtc gaactgcatt tccacgaccg gacgaaaacc ggccatggcc atgccgattg 3001 cgaatccgat gatgccggac tcggcgaggg gagtgtcgaa gcatcggtcg tcgccgaagt 3061 cgcgggtgag gccgtcggtg acgcggaaaa cgccgcccag ggtgccgacg tcctcaccga 3121 agacgacaac gttgtcgtcc gcagccagag agtcgcgtag cgaggtgttg agggcctgag 3181 ccatcgtgag catgggcatg gtcagtgtcc ccctgtgtca gcggcttcgg cagcaagttc 3241 ggtttcgagt tgggcttgtt gctcacgcaa ttgcggtgtc tgctcggcga agacgtaccg 3301 gaacagatcg agcggatcgt gcgggcggtc gacgttcatg ccggcgcgca gtgtggcggc 3361 cgcggtttct gcctctgcag tcagcgattc cacgaacgcg tcgtcgatca gatcgtgtgc 3421 gcgcagatac ttttcgagac gcggcaacgg atctcgcccg agccaggctt ccacctcggc 3481 gctgtcgcgg tagcgggtgg cgtcgtcggc attggtgtgt gcgtcgatgc gataagtgtg 3541 tgcctcgacg atcaccggtc cgtttccgct gcggacgaag cgtgccgcct cgtccatgac 3601 ggccatcatc gcgacgggat cgttgccgtc tacctgctcg ctgccgatcc cgtagccgac 3661 gcccttgtgc gccaatgtcg gtgctgcgct ttggcgcgac agtggcacgc tgatcgcaaa 3721 tccgttgttc tgcaccagaa agatcaccgg cgctttgaag acggcggcga agttcagcgc 3781 ctcgtggaaa tcgccctcac tggtggcgcc gtcaccacag agcgccaagg cgacggtgtt 3841 caagccccgt cgagactccc cgtaggccac ccccgctgca tgcagcaatt gcgttgccag 3901 cggggtgcac tgcggcgcac tgcgcagtgc gaccgggtcg tatccgcagt gccagtcgcc 3961 ggcgagcatg ctcagaatct gcaccggatc gacaccccgg gcggccaacg ccatcgaatc 4021 ccggtaggtg gggaagagcc agtcactcgg ctccagactc atcgctgccg cgatctgaca 4081 tgcctcctgc cctcgcgagg acggatacac ggccaggcgc ccctgtttgg tcagtgccgt 4141 agcctgctgg tcgaaacgcc tgcccaggaa catctttcga tacatcgcga tcaggcgatc 4201 gtcgctcggt tttgcgtagc gggcctcgct gcgcgtcagt tctccggcag ggtcgaggta 4261 ctgcaccgca gaatcggccg gcaggaactt ctgataccgc agttccccca agggctcgac 4321 aagtgtcact tctacctcca gaagacgttg tgtccatcag tattcgcact tcggcgacgt 4381 aatggcagca agcgtcgagg attcaagaca aacggcctat catgaggatt cgggcagcaa 4441 tatgtccatc aaccgactcg actaccgaac caggaatgga cagatggcca ccgaaccgca 4501 cgctctcgat gcgatcgacc gccgcatcat cgccgaactg gtcgcagaca gtcgcatctc 4561 catgcgtgca ctggctgaaa aggcgcacat atcccgcgct cacgcctacg tccgcatcga 4621 aaggctcgag gacgccggaa tcatcaccgg attcacgacg cggatcgccc atgagaaggc 4681 cggactcggc tcctcggcgt acgtggcact ctcgatccgg cagcactcgt ggcgaggtct 4741 ggcggcgtcc ttgaaggccc tgccgtttgt cgagcacttc tccctcctcg gcggcgactt 4801 cgacgttctg gtgctggtcc gtgccccgga caaccaggaa ctacgccaca ccgttctcga 4861 agaactccag ggactcgacg gggttctctc gaccaaaacg tggctgatct tcgaagaagg 4921 caacggtccg ggacccaact ggctgcccgc ctgatccgtc tatagtcgcc gttagcagtt 4981 gcacacggga ggtgattcga atgaccctgt acggcctggg aatcggttgg cgccccgagg 5041 tctccggaat catcgcgcag ctcgacggcc tggcattctg cgaagtcatt gccgaatccg 5101 tcggaaacga gataccgccg cacctcggct cgctcggagt tccggtgatc ccgcacggaa 5161 tctcgctctc acttggcggg gccgaggcgg tagatcaggc acggatcgat caccttgcgc 5221 atgcagctca gctactgggc gctcccttgg cgagcgagca catcgcattc gtgcgtgcag 5281 gcggcatcga ggccggccac ctgctcccac ttccgcgcac ctacgaagcc gtcgacgccc 5341 tggtacgcaa catcacccgg actcaggacg gtctaccggt gccgttcgcc gtcgaaaaca 5401 ttgccgccct gttcgactgg ccggacgacg agttgaccga aggggagttt ctcgcggaga 5461 tcgtcgagcg caccggagcc ctgctggttc tcgacatcgc gaacgtctac gccaacgctc 5521 tcaatcgcgg gcgtgatccc tggaccgagc tgagccggct gccactcgag cgaatcgcct 5581 actgccacat cgccggtggc agtctcggcg acggaaaata ccacgacacg cacaccgacc 5641 cggtgcccga agccgtgctg gaactccttg cgcagttcac cgccgccggt caccatcccg 5701 cgatcatgct cgagcgcgac ggaaactatc cgccggcact ggaattgctc gacgagctcg 5761 atgccatcgc cgacgcagct ggtctggatc gcatcaccgc gggcaccgat tggtcacgca 5821 catgagcacg ctcggtgagc gtcaagaggc gctgattcgc gcactggtgg cgggtggcga 5881 actgcccgaa ggcttcgaca aagacgaagc cgccgttgtc tcggcggcac tgctccgaaa 5941 gcgcgcagga gaagtggcgc accaccttcc ggtcgtgcgc cacactctcg gcgatcgtta 6001 tcttcagttg ttcacagcgt gggcgggcgg acggccgaag acgtcttcgc gttcggatgc 6061 ccaggccttt gtcgcccatc tccaggacat cggcgaactg cctcgcccgc cctggtatca 6121 gaggtttcga aagctctcac gcaagtgaga caacagcttc gctggtgtac atgaggaacg 6181 tcatcgattc ctggaagtac agctcgacgg agttcgcatc gtgcgagaga tagccgatgg 6241 acaggtcctg gcccagatgc agttcgtagt caccgccacg cgtcgacagc aggaaagcgc 6301 cgtcgatggc cggggcccag atgatctcgc cgtcgagtac gcgtcgcagg tgctcgcgga 6361 tggggtagcc gtgatcggag gtctcgttga tcgcggtgaa cgcatctgca ttcaggagca 6421 gtgagtacgg tccgttgaca ccggccaagc gcagcgacgt gatcgcctgg ctgatggctt 6481 ccgggtagtc cttggcgtcg atggggagct tcagttccgg gttggaaccg ctggcccgca 6541 cgccggtgat gctggctgcg gggtatccct cgaagatggc gcggtcctcg gcgaacgcga 6601 tctgcttggc cgcgtccttg acgggctgcc agtcggagtc cttggcgccg cgttcgacgt 6661 cgtcgatggc ctgacggctg acggtgaaag ggacccgcag ttcgatgatc ggctgggact 6721 gacgagcatg tgcgatgacg ccgtcggcca agggattgat cggcacctga tgcccgagtg 6781 ggatggccgc gagatcgtca cccgacggtc cctccacatc cacgacacga cgaccggcga 6841 cgtgacgctt gaaggtacgg ctcgcctctt cttcgatctc ggcccaggct gctgccgaaa 6901 tgggtgctag gtcgcggtgc aggtttgtca ttgctgggca ctccttttga ggctgccgat 6961 tccgagagaa ccgtcagaga tgggcgctgt gaacgtcgct tcgggaacga gcctggtcgg 7021 caagtccggg ggatcgtcga gaaagtccgc cgtcggaacg aagaaattga ttccggtgac 7081 ggcggtggag aaatcgagaa tgcggtcgta attgccgacg ggattcccga tgaacatgtt 7141 ggtgagcatc tgctcggtga ccgtaggcga cgccgagtaa ccgatgaaat aggtacccat 7201 ctcagcggag ccgacgtggc cgaacggcat gttgtcccgc agaatctgga tctgctcacc 7261 ggattcgtcc tcgatgacgg tgagtgcggt gtgggagttg gccggcttgg tgtcgtcgtc 7321 catctcgaga tcttcgagct tggagcgtcc gatgacgttc tcctgctcct cgacgggcag 7381 ggagttccac tccgacatgt cgtggaggta cttctggacg atgacgtaac tgctgcctgc 7441 gaaatccgga tcctcgtcac cgacggtgac cgcgacgtaa gcggcctgcc cggacgggtt 7501 ctcggtaccg tcgacaaaac ccatgaggtc gcgttgctcg aaatacttga acccgtggac 7561 ctcgtcgatc accgacgcgg ccccggccaa cctgttggtc acgaccgtgg cgaactcgaa 7621 gcacaggtcc atctgacggg cccggatgtg aaacaacaga tcgcccggcg tgcgcggggc 7681 gtggtgtttg tcaccggtca actcgacgaa cggatgcaat tccgccggcc gtggaccgtc 7741 gaaaagtcga tcccacgcat ccgaaccgat gccgacgacg gcagccaagc caccggcggg 7801 aacccggaat ccgatcgagc gttgcagccc ggaaatatcc gccagaacgt cgtgaactgc 7861 ctgctcacca ccctcgtcga tggtgaaaac caggaagatc gcagcttccg tgagcggggt 7921 cagcaacggc tgcggtatcg cgggaagggc cacttcggcc tcgtcgatct cactcgtttc 7981 cgccatgcag accagtatgg acgattccgg cagtcccggc atcagtagtg ggtaagtcgc 8041 gtcagggtgt cgcgtcaggg tgttgcggcc tgggcgcccg cgtcgaagcc ggccggaatc 8101 tcgaagatct ttccgtcgaa cccggcgcgg tagagatgcc cggccgcgaa accggaattg 8161 ccacgcccgt aggtcaacac actcgacgct tgcagtccgc tggtgagcga gcagtactgg 8221 ccgggtccgt cgatccgcca cacctcacca ctgatgtccg tgggaacaat tgcgcgatct 8281 cgcgagtcca aggtcaaacc atctggggca gagaaggttt cgaaaccacc gagatcgacc 8341 aacgactccg aatatcccgg attgtccacg gagatgcggc tgacacccgg gtttgcgaac 8401 gtgcgcgaaa cgtagagcca gcgattatcg gcgctcaggg cagcgccgtt tgcactcggg 8461 aaggttgccc acgccgggtc gacggaacca ttcgggctga cctttccgat cagcgaaccg 8521 aaatcgttgg tggcgtagat cgtcccgtcg tgcgcgacgg caaggccgtt tgcagcgctc 8581 agaccaccgg ctatcggcgt cacggcgccc gagttcacgt ccacccgcgc gaccgacgca 8641 gcacgcgtca gatcgccgat cagcacgccg gcgtttgctc cggtacccac cagcagtgaa 8701 ccgtccgggc tccacgcgag tgcgcccgca ccgccgggaa ccgtcgcgat cggaactgcg 8761 gcctgaccag gcccgtcgac gcggaagatc cgcccactca ccaggtcggt cgtatacagg 8821 cgcccttgcc cgtccaccgt gacaccctcg agggccgcac ccggaatctg ggcgctgagt 8881 atggcgggtg acgatccagg gcaaggcgga gcggcggcgg caccggacac gcccagagcc 8941 agaagcgaac cggcaacaag agcaccggtg atacctaccc gaataccgcg atcgaagatc 9001 ctggtgcgca tctgcgcaac gctagacctt cggcccgatt tcgcctagag cgaccgaagg 9061 aaagagatca aaagttcatt gacctcggca gcgtgttctt gttgtacgaa gtgccccgca 9121 ccgccgagga gatgtacacc ccgcaggtcg gggacagtgt ccttcattcg gtccagtgcc 9181 ttgggaccgc tcatcgcgat gaccgggtct tccgcgcccg caacgaagag cgtcgggacc 9241 tcgatgtgcg ctccgtccag atccgcgctg cgttcccaat tggcgtcata cgctcggtac 9301 cagttcaagc caccggtgaa acctgttgca gtaaaggcct ttacatagac atccaaatca 9361 tcctcggtga gccacgacca cggcaacgcc ggcgcctcgg gaagtacgtc gagatagccg 9421 tttccttcgg aggggttctt ccacacatcg aggtaccggt aagccccgga caaggcgtgg 9481 aagagccgct gaaggaattc acgtggacgg gcgtcgagtt cgcgctccgc aacacccggt 9541 tcctgaaaat agtgaatgtg cagaaagtgc ttgcgcgcca aggcggcgta gatggccgag 9601 ggcttggccg ggaatctgtc cggcgcatag ggaaccgcca aggagaccag tcccgcaacg 9661 cgatcggggt gacgaagcgc tgtcgtccac gcaaccggag cgccgaaatc gtgtccgacg 9721 aacaccgctt tgtcgattcc gagagcgtcg agtaggtcga tcagccggtt cgcgacggcg 9781 tcgttggtgt agtcctcgat cgcgtcgggg cgggtagtgc cgccgtaacc gggcatgtcg 9841 agtgcaatcg accgaaaacc cgcttccgcc aacgctgccg actgatggcg atagctgtat 9901 ccgaggccgg gaaagccgtg gcacatgacc acagctgtcc cggaaccgga atcgtcgatc 9961 tgccaacaga atccgtcgac gtcgatcgtc ttgaccgtca tgtcaggcgg tgatcatgtc 10021 ttcctggttc cagaatgcac gcatcgacag gatctttccg tcgtcgtcga aggtcatgac 10081 gtcgatgggt gcgatggtca gagtctgctc gccgagcttg gtgatcagcg agaagtggaa 10141 tgccgcttcg tttccggcaa tgcggagggt ctgaaggtga gcttcctggt cgaagttctc 10201 gatcacggag tagaactcga gaatggactc gcgggtggtg cgcacgtcgg taccaaccgg 10261 gtcttcaacc gttgcaccct ctgcgtacag ggccacaacc tgggcagcag tgcccgtcgc 10321 aactgtctcg atgtaggcgt cgacggtctt gcggattacc tctgcggacg gtgccatggt 10381 ctacccctcg tagttagaac aggttctaat tctgaggata acctacgact ctgttccacg 10441 ggtcagcttt cgagcacctt gcgcaggcga gccttcttcc ggttctcccg cacgatccag 10501 acgacatcgg tgtcgtccga cgcccccagg gagacgaatg ccggcagggc attctccgga 10561 tccgccgcaa ccgcgacact ccagcagtat ccgagcgcct gccggagagt ccgtgtgtcg 10621 gcgtcacgac gggaatcagc cggttgcgag acgatccact tggtagcgtc tcggcacacc 10681 tgaacggcca gcgcagcgtt ggtcttgtcc ttcagcaacc gtggctcgca gatccccgca 10741 gccgctgcgc gcagcgtcaa tggatgagcg gagcgtgacc actgctcgat gatcgcccgc 10801 aataattgcg gatccgtgtc gcctatcgcc tgaacggcca tcgcggcagc ttcacgtgca 10861 cgccacgacg cactggtggt tgcctccgtg agactgttca gaacagcctt gtcgcgtgaa 10921 cgaacaagga tgaatccgag cccgacgatc ccacagcagc ggatgtattc gtcctcggac 10981 tccgccgcca gcaggatgtc gtcgtcatcg gcgccgcgcg cgaactcgag catgagccca 11041 aggttcacgc gcgggccggg caatcctgaa cgctccgtca ggtaactcca cagttcgtca 11101 ccgtccagat ccgcgaacgt catgggagtc ctgctttctc gagtgcgggt gcacccatca 11161 tttcaccgaa agccctctca cgacgggagt tcgaagctca cttgcttccg tcgtcgcgca 11221 tagaggaatc caccgacaaa cgcgagcacc agcaccccgg tcatcaccac ggccagaatg 11281 ttgccgccca gtaggagcgg aaggtttccg gcaatggcga tcgcgaatcc gatcagcccg 11341 acaaggccca agctcggggc aagtttggtg tgccagaatc gctgatcgac cttcgtacga 11401 tgaaagaaga cgagcaccgc aaggcacgtg agcaacatga gagccacgat ccccaccgtt 11461 gcaacgccgg cgaaccacgc gaagatctgt gtcaccggat ccagaccgat gacgatgcaa 11521 gctccgagaa gtaccagagc acagcaggat tgcaccactg acgcgatgtg cggcgaacca 11581 tgcacgcggt gcgtccgccc acctgcgccc ggcagaacgc cggtgttcga cagaacgaag 11641 atgtatctgg ccaagacatt gtggagagac agaactgcgg caaagagact ggacaccaac 11701 agaatttgaa tcaggtgggc tgtcaccgtt ccggcaaagt cggtggcagt ggtgatcagc 11761 atgccgtcgg ggtcagtacc cgcaatgtcc actgcaccct tgtcgcccca ggctgtcaca 11821 accgcccaac tggagagagc gtagaagccg ccgatgagta tcaaggcggc gtatgtcgct 11881 cgcgggatag tccgcgcggg atccttcgct tcgtcacgga agatcgccgt c //