LOCUS       NZ_JAPWIK010000002     10888 bp    DNA     linear   CON 14-FEB-2024
DEFINITION  Rhodococcus qingshengii strain IEGM 766
            NODE_2_length_1167099_cov_313.895192, whole genome shotgun sequence.
ACCESSION   NZ_JAPWIK010000002
VERSION     NZ_JAPWIK010000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Rhodococcus qingshengii
  ORGANISM  Rhodococcus qingshengii
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus; Rhodococcus erythropolis group.
REFERENCE   1  (bases 1 to 1167099)
  AUTHORS   Krivoruchko,A.V. and Elkin,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2022) Lam, Institute of Ecology and Genetics of
            Microorganisms, Ural Branch of the Russian Academy of Sciences,
            Golev Str., 13, Perm, Perm Kray 614081, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: SPAdes v. 3.15.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 311x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/14/2024 02:58:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,449
            CDSs (total)                      :: 6,385
            Genes (coding)                    :: 6,320
            CDSs (with protein)               :: 6,320
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 5 (23S)
            tRNAs                             :: 54
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 17 of 65
            Pseudo Genes (incomplete)         :: 52 of 65
            Pseudo Genes (internal stop)      :: 6 of 65
            Pseudo Genes (multiple problems)  :: 9 of 65
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:17:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 464541
            Orig. end                         :: 475429
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPWIK010000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10888
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10888
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10888
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5888](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5888)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(979..1692)
                     /locus_tag="O4271_RS08325"
                     /old_locus_tag="O4271_08325"
     CDS             complement(979..1692)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /locus_tag="O4271_RS08325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08325"
                     /product="FCD domain-containing protein"
                     /protein_id="WP_269610782.1"
                     /transl_table=11
                     /translation="MPDSRIAPTARNIPLSVQIAEQLRSQITSGNWELGEKIPGEHQLV
                     EMTGASRNTVREAIRGLVHAGLLEAHPGDGTYVCASSELEIALQRRAAAENVLEIFEAR
                     EALELYAARLAAERALPEDIDRMTRALDGRDAEPDVAKSVEYDLDFHLEMFAASGNQLI
                     TDIYRSLDRSDTMSIPQGKSDKETRQLFIGPWTDDDPHRQLLAAIRDRDPDGAAAAVVR
                     LMDHSRSLFLQNMPK"
     misc_feature    complement(1729..1744)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     gene            complement(1912..3030)
                     /locus_tag="O4271_RS08330"
                     /old_locus_tag="O4271_08330"
     CDS             complement(1912..3030)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745611.1"
                     /locus_tag="O4271_RS08330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08330"
                     /product="hypothetical protein"
                     /protein_id="WP_050656070.1"
                     /transl_table=11
                     /translation="MLTADILLSGPRGRRMLLAYAIDAERALRPEPGDDMFRINAHRAA
                     YHLDIGQGTSQVLFGPGAEEARQTIVTAEGVARSLTEISLPDVTAVDLRSALAEAVDAA
                     RYWQDPDGEDVLVATEVMRDELRRVAEHIAGSECARWWSTSLASSDQWSILSRGEESPD
                     TDEVPAAQQLLSEWRTSTLESEARAERDRPVDPAANWSGYWWSNPPTQGSTRALFDGTP
                     AGLWFVEDSMGWERVVVRALRPPTGARIYEIDSAQAWSELCRRFPLEVTAETRQVWYRT
                     TGRSGRWVIPDWVQVAERFEGVHLTVTGYLAAAGTAIEVDTETASVIAGWAPDETYWFT
                     DAATYISEPCAWVLHDGPEVTDQPAWTLESQQ"
     gene            complement(3389..4231)
                     /locus_tag="O4271_RS08335"
                     /old_locus_tag="O4271_08335"
     CDS             complement(3389..4231)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (78-162). E-value:
                     6.6e-07. Score: 20.6. Matches aSDomain:
                     nrpspksdomains_O4271_RS08335_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940604.1"
                     /locus_tag="O4271_RS08335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08335"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_065351496.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 6.3e-13, bitscore:
                     44.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.7e-07,
                     bitscore: 25.6, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLVLGATGFIGSAVMKQLVSRHGNAAIGFVRTATAAKQFGREG
                     IATVIGDINESASLRVAMSQASTVICCVSYVGDDEQRCVHVNQHGVQNVASMAATVGVE
                     RLLYVSTAAVYGPGPFRDLPVNGAPLSPFSPASRTRALAEKFVRDAGGLVVRPHLVYGP
                     GDRWFLPTLTRIVSRLDSIIDGGSAVLSVVDVDLLARSIYELSIEQQFRYGSTLHVNEP
                     VPRTVIDLLEQHARETNWTLPQNSIPRADAVKAAAQLSLDMHQIDMISLDHWFRSRLY"
     aSDomain        complement(3746..3997)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O4271_RS08335_NAD_binding_4.1"
                     /evalue="6.60E-07"
                     /label="O4271_RS08335_NAD_binding_4.1"
                     /locus_tag="O4271_RS08335"
                     /protein_end="162"
                     /protein_start="78"
                     /score="20.6"
                     /tool="antismash"
                     /translation="DEQRCVHVNQHGVQNVASMAATVGVERLLYVSTAAVYGPGPFRDL
                     PVNGAPLSPFSPASRTRALAEKFVRDAGGLVVRPHLVYG"
     gene            4318..4962
                     /locus_tag="O4271_RS08340"
                     /old_locus_tag="O4271_08340"
     CDS             4318..4962
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729741.1"
                     /locus_tag="O4271_RS08340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08340"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_003940238.1"
                     /transl_table=11
                     /translation="MQERAVLTRQAVILGAAKSFEKFGYSASLGTILQHGGVSKGAMYF
                     HFASKEELAHAVIAAQHGMAMEGTRRVAAHSDIAVETLVLVSQEMARQLVTEPIARGGM
                     RLTMEIGSIQGPIEQPYLDWIEAIKQVATQAAEAGDIVPGTDIDALARFVVGSFTGVQI
                     LSEMLTGRTDLYERLSQMWNLVLPTIVREDKLAHVMQLAALAPREWEAPSA"
     gene            complement(5001..5888)
                     /locus_tag="O4271_RS08345"
                     /old_locus_tag="O4271_08345"
     CDS             complement(5001..5888)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729740.1"
                     /locus_tag="O4271_RS08345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08345"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_269610787.1"
                     /sec_met_domain="AfsA (E-value: 1.5e-18, bitscore: 62.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYTRTVPRHLVHRHAVAEVFLTDYRIESDSAYQVAVQLPVSHRI
                     FDVSSEQHDPLGVAEAFRQAVVLLCHTRYEIPLHYKFLMETFSFEMLDALDVQSVAEPL
                     SFSMTVDNLTHRDGAVSGVEVSGILSEGNRHVARCNAVARGVSPEGYRRIRQGREDHRP
                     SRRILPSGAAVVGSERVGRLTDGDVLISTDPARHAMFCAPDPHNHSLFDHPVDHIPGMI
                     LFEAARQALRFHIGNPATKIASLSAVFPRFTEWETPCEVSTIPLDASGHVHRVRFTQEL
                     ETTAELEITVADQS"
     gene            complement(6057..6716)
                     /locus_tag="O4271_RS08350"
                     /old_locus_tag="O4271_08350"
     CDS             complement(6057..6716)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729739.1"
                     /locus_tag="O4271_RS08350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08350"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_050656054.1"
                     /transl_table=11
                     /translation="MTLSSTVTLYTTHSDQGFANAIAAGISARAYSEPQTADMSLLRHH
                     TSDHGRALVLLPLGGVDWQVKELSALEGSVLVGDLSSGNSMIRGLAAGAHAVLNSQLPF
                     TSLLDELQAAINPNSARSAHDKSSLVQRIRLRTEEFARISSLTDREFEIVGLLYGGSNV
                     DSIARTAHIAPTTVRAHIRSILQKLGVHSQLEACAMLRRSGHDARRHPLELRIGRF"
     gene            7233..8732
                     /locus_tag="O4271_RS08355"
                     /old_locus_tag="O4271_08355"
     CDS             7233..8732
                     /EC_number="1.14.13.-"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1092: hypothetical
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745606.1"
                     /locus_tag="O4271_RS08355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08355"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_003940522.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 4.8e-09, bitscore:
                     31.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQHVDVLIIGAGLSGIGAACHLIREQTGSTYAILERRENIGGTW
                     DLFKYPGIRSDSDMLTFGFGFRPWIGTKVLADGASIRDYVEETAKEYGVTDHINFGRKV
                     VAMDFDRATAQWSVTVLVEATGETETWTANVLVGACGYYNYDKGYRPAFPGEDDFRGQI
                     VHPQHWPEDLDYTGKKVVVIGSGATAITLIPSMAPTAGHVTMLQRSPTWIQALPSEDPV
                     AKGLKLARVPDQIAYKIGRARNIALQRASFQLSRTNPKLAKKLFLTQIRLQLGKNVDLR
                     HFTPNYNPWDQRLCVVPNGDLFKVLKSGKADIVTDRIATFTEKGIVTESGREIEADVIV
                     TATGLNVQILGGATMSIDGEPVKLNETVAYKSVLYSDIPNFLMILGYTNASWTLKADLA
                     ASYLCRVLKIMRKRDYTTFEVHAEPEDFAEESLMGGALTSGYIQRGDGEMPRQGARGAW
                     KVVNNYYRDRKLMHDAEIEDGVLQFSKVDIAVVPDSKVASA"
     gene            complement(8815..9285)
                     /locus_tag="O4271_RS08360"
                     /old_locus_tag="O4271_08360"
     CDS             complement(8815..9285)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729737.1"
                     /locus_tag="O4271_RS08360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08360"
                     /product="hypothetical protein"
                     /protein_id="WP_064112397.1"
                     /transl_table=11
                     /translation="MEPLEPMRPVSVPADTHKSTPIWKSAPVTLGTVGVFAYALMSART
                     GVVEVALGAAIAIAGAALYCMSVMRTIRENSSSRVPLLGTPPVSPRDVDLLAGAGMPLI
                     MGGSLLAIRAAGWTWPYLYLGLLAVIMVATYVLPVVVHNRRLRKRTHRSQTP"
     gene            9363..10052
                     /gene="lexA"
                     /locus_tag="O4271_RS08365"
                     /old_locus_tag="O4271_08365"
     CDS             9363..10052
                     /EC_number="3.4.21.88"
                     /GO_function="GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="lexA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940361.1"
                     /locus_tag="O4271_RS08365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08365"
                     /product="transcriptional repressor LexA"
                     /protein_id="WP_269611392.1"
                     /transl_table=11
                     /translation="MSGPLTTILVVTDYDDLDMFGGLDASSLPLRQQQILMTIRDLVAA
                     NGCTPSTRQIGDAVGLRSTSTVSKHLKSLEEKGFLRRGAAMARQLDVRPFLVGAKEQSS
                     SNTVTVPVVGDIAAGAPILAEEHADEVLALPRELVGSGTVFGLRVRGESMVDAAICDGD
                     VVVVRRQDEAHSGEIVAAMIDGEATVKVLRRRDGHVYLEPRNPAYSVIDGDHAVILGKV
                     VSVMRRI"
     gene            complement(10066..10479)
                     /locus_tag="O4271_RS08370"
                     /old_locus_tag="O4271_08370"
     CDS             complement(10066..10479)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003939933.1"
                     /locus_tag="O4271_RS08370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O4271_08370"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_054825997.1"
                     /transl_table=11
                     /translation="MEILKPQPTSKAPSDWFTGDVWWDVIYAGQEPSRMRANMVRFAPC
                     ARTDWHSHAVGQTLHIVSGTALVQARGGEIVEVHPGETVYTPAGEEHWHGAAPDRFMSH
                     LALWEGPGDGGTETTWGDKVTDEEYGGPRARRR"
ORIGIN
        1 agaactgcca ctgcggcgac cgacaccagc ccgaggacgg ctcctctctg atccttgggc
       61 agtttgtgca ggagcactgc tgtgaggagg ctgccgacga tcgagaatat ttgcatgatg
      121 ccttgatgca gtccggcggt agccgcggta gtgccgtgtg attcttcaat ggtgggccac
      181 catgtcagaa cggtgtagaa gaagagagat tggacgccca tgaatattgt gatctgccac
      241 ccgagtgctg agcgccaggg ggagcgatgg ccgcctggat gggaatccaa agccgcagca
      301 tgatcgtgtt tcggaagcgt gcgtcggcga agttggggaa ggaacacccc aaatcctatg
      361 agagccatag cagcccagat tccgaatgcg atccgccaac cgtgatcggt gagaccggcg
      421 atcggcacgg caagtccgga tgcggttgcg gcgacggctg cagagaccgc cgagaagatg
      481 ccggtcaaac ggccggcttc ggtcgggaag tctcgtttga ggagcgccgg cagtagaacg
      541 ttcattgttg cgatggacat gccgagcgat acggtaccga tccacagcgc aggcatccac
      601 ggaacagagc ggatcacgat cgctaaggcg agcatgccga gtgctgctgc gagtgtccgt
      661 tccattcccc accgagctgc gatgaccggg gcaacggggg agaacactgc gaagcacagc
      721 agtggaaggc tgatcagcgc tgatgctgtt gtcgacgaga tatcgagatc ggtgcgtacc
      781 tcaccgagta gcgggccgac gactgtgatt gaggctcgaa gattggctgc gatgagaaga
      841 atgccagcga taacgagtcc tcggcgcatt atcggtatcg cgctcggggc agacgccacg
      901 ttttcggtgt cggagatgac gttagccata ggtgtgccct tctgagattg tctgtgcgct
      961 gaacctcttg tgtgtcagct acttcggcat gttttgcaga aagagggatc ggctgtggtc
     1021 catcagtcgg accactgcgg cggctgcgcc gtccggatcg cggtcacgga tggccgcaag
     1081 cagctgccgg tgtgggtcgt cgtcggtcca aggtccgatg aacagctgtc gagtctcctt
     1141 gtcggactta ccttgtggga tgctcatggt gtcagagcga tcgagactgc ggtagatgtc
     1201 cgtgatcagt tggttcccgc tcgctgcgaa catctccagg tggaagtcga ggtcgtactc
     1261 gacggatttg gcgacgtcgg gctcagcgtc tcggccatcc aaagcccgag tcattcggtc
     1321 gatgtcttcg ggcagggctc gttcggctgc gagtcgggcg gcgtagagct ccaaagcttc
     1381 acgggcttcg aagatttcga gcacgttctc cgccgctgca cgtctttgaa gtgcaatttc
     1441 gagttctgag ctcgcgcaga cgtaggttcc gtctcccggg tgtgcttcga ggaggccggc
     1501 atggacgagg ccgcgaatgg cctcccggac cgtattgcgg ctcgcgccgg tcatttcgac
     1561 aagttgatgc tcgccgggga tcttctcccc taattcccag ttgccagaag tgatctggga
     1621 ccgcagttgt tccgcgatct gcactgaaag tgggatgttg cgtgcggtgg gtgcgatacg
     1681 ggaatccggc atgagtcaaa tgtaggacat ttggcgattt gtggataggt ttacctttgt
     1741 gatctagggc actcgggttc gggtcggcct gggcggggta ccgcccttcc gcatgtctgc
     1801 gtgcacgtga gtgctcctgc cgaccgcgca cggggcagtt gactcgactc gaagggcaac
     1861 tgcgatcgat tcctcacgga acctcatgcc tagcagaccc tcacgtgcgg gtcattgctg
     1921 cgattccagc gtccatgcgg gctggtcggt cacttccggg ccgtcgtgca gcacccaagc
     1981 acacggctcg gaaatgtagg tggcagcatc ggtgaaccag tatgtttcgt cgggtgccca
     2041 gcccgcgatg acgctcgccg tctcggtatc gacctcgatc gcggttcctg ctgccgcgag
     2101 gtatcccgtc acggtaaggt gcactccctc aaatctttcc gcgacctgca cccagtcggg
     2161 gatgacccac ctgcctgatc ggccggtagt ccgataccaa acctgccgcg tttctgcggt
     2221 gacctcgagc gggaaccgtc tgcacagctc cgaccaggct tgcgcgctgt cgatctcgta
     2281 gatgcgtgcg ccagtcggtg gccgcaatgc tcggacgacg acccgttccc atcccatgct
     2341 gtcttcgacg aaccacaagc ccgccggagt cccgtcgaac agcgccctcg tcgaaccctg
     2401 agtgggtgga ttcgaccacc aataacctga ccagttcgcc gcggggtcca ccgggcggtc
     2461 acgttcagct ctcgcttccg attccagggt gctcgtgcgc cactcgctga ggagttgttg
     2521 cgcagcaggc acttcgtctg tgtccggaga ttcctcacct ctgctcagaa tcgaccattg
     2581 gtcggatgag gcgagcgagg ttgaccacca ccgcgcgcat tcggagccgg cgatgtgctc
     2641 ggcgacgcgt cgaagctcat ctcgcataac ttcggtggcg acgaggacgt cttctccgtc
     2701 gggatcctgc cagtaacgcg ccgcatcgac cgcttctgcg agggcggagc gtagatcgac
     2761 ggcggtgacg tcggggagcg atatttccgt gaggctgcgc gccacgccct ccgcggtgac
     2821 tatggtctgc cgggcctctt cggcgcccgg gccgaacaac acttgggagg ttccttgtcc
     2881 gatgtcgagg tgatacgccg cccggtgagc gttgatgcgg aacatgtcgt cgcctggctc
     2941 gggacgcagg gcgcgttcgg cgtcgatcgc atacgcgagc agcatccggc gtccgcgcgg
     3001 gcccgacagc aaaatatccg cagtgagcat ctgttctccc gttttctggt tcagatgacg
     3061 gtagccgagg tcgttgagac ggagaacttt ctcctttgat gtcggaaatc ccgacggtcc
     3121 cgtcgcgcac gacatgatcg ccacacactg cattcggcgg cgaaggcgga cggaagggag
     3181 ccccaggctg ctgccagtcg cgtgaacgcc gcttctttgg tgtgcgcgat cactgccgtc
     3241 tttgtgtagc cgtggttttc gtcgatcaga gctctctgtt tgcctgtagt gactgcgaaa
     3301 taggtcgtat tgattgctga ggctgaacaa tggagagggc tgaaaaggtt gggagacagg
     3361 tgttttggcg agagtgatcc gtgtgccatc agtacagtct cgaccggaac cagtgatcga
     3421 ggctgatcat gtcgatctgg tgcatgtcga gactcagctg ggcagcagct ttcaccgcgt
     3481 cggcgcgggg gatagagttc tggggcagag tccagttggt ctcccgcgca tgttgctcga
     3541 gaaggtcgat gactgtccgc gggacaggct cgttgacgtg aagtgttgag ccgtagcgaa
     3601 attgctgttc tatcgacaac tcgtagatgg atcgggcgag taggtctacg tcgactaccg
     3661 acaggacagc gctaccgccg tcgatgatcg agtcgaggcg agagacaatt cgcgtcagtg
     3721 tcggaagaaa ccatctgtcg ccgggtccgt acacgagatg cggccggacg accaggcctc
     3781 cggcgtcgcg cacgaacttc tcggcgagag cgcgcgtgcg actggcaggt gagaaggggc
     3841 tgagaggtgc gccgttgacc ggcaagtctc gaaacggtcc cggcccgtac acggccgcgg
     3901 tactgacgta gagcaagcgt tcgacgccga cggtggcggc catcgacgca acgttctgta
     3961 cgccgtgttg gttgacgtga acgcaccgtt gttcgtcgtc gcccacgtac gacacgcaac
     4021 agatgacggt cgacgcctga ctcattgcaa ctcgtagaga agcactttcg ttgatgtcgc
     4081 cgatcacggt ggcgattcct tcacggccga actgtttggc tgcggtggcc gtgcgaacga
     4141 aaccgattgc agcgtttccg tggcgggaaa ccagctgttt catcactgcc gagccgatga
     4201 aaccagtggc cccgagtacc agagtcgtca cggtgtgtcc gtgtcgagag cgttgctgcg
     4261 ggctctttcg acgtatcgtt gagattcatt ccgtcggcaa cgagagctgg gtagatcatg
     4321 caggagcgtg ctgtcctgac gcgccaggcc gtcattttgg gtgctgcgaa gagcttcgag
     4381 aagttcgggt actccgccag tttgggcacg atcttgcagc acgggggtgt gtcgaagggg
     4441 gcgatgtatt ttcatttcgc gtccaaggag gagcttgcgc acgcggtcat cgcagcgcag
     4501 cacggtatgg cgatggaggg tacccggcgg gttgctgcgc actccgacat cgcggtcgaa
     4561 accctggtgc tcgtttcgca ggagatggct cggcagcttg tgaccgagcc catcgcgcga
     4621 ggtggcatgc ggttgacgat ggagatcggc agtattcaag gccccatcga acagccgtac
     4681 ctcgattgga tcgaggctat caagcaggtg gcaacccaag cggccgaagc cggcgatatc
     4741 gtcccgggca ccgatatcga cgcgttggct cgatttgtgg tggggtcgtt caccggagtg
     4801 cagattctct cggaaatgct gacgggcaga accgatctgt acgagcggtt gagtcagatg
     4861 tggaacctgg tgctgccaac gatcgtccgc gaggacaagt tggcgcacgt catgcaactg
     4921 gcggcgcttg caccgagaga gtgggaagca ccgagcgctt gacgtggaag acgcgacgag
     4981 aagaacgtgt tcgttccctc tcatgattgg tccgcaactg taatttcgag ttccgcggtg
     5041 gtttccagtt cttgagtgaa ccgaactcgg tgcacatggc ccgacgcgtc aagggggatc
     5101 gttgacacct cgcacggcgt ttcccattca gtgaacctcg gaaagaccgc cgagagtgag
     5161 gcgatctttg ttgctgggtt gccgatgtga aatcggagcg cttgtcgggc ggcctcgaaa
     5221 agtatcattc ctgggatgtg gtcgacgggg tggtcgaaaa gggagtggtt gtgcggatcg
     5281 ggagcgcaga acatcgcgtg ccgagcagga tcagtgctga tgagcacatc tccatcggtt
     5341 aggcgtccga ctcgctccga tccgaccacc gcggctccgg aaggaaggat gcgtcggctc
     5401 gggcggtggt cttctcgtcc ttgccgaatt cgacggtatc cctcggggct gactcctcgc
     5461 gcaacagcgt tgcatctggc gacgtgccgg ttgccctcgc tgaggatccc cgacacttcg
     5521 acccccgaaa ccgctccgtc gcgatgagtg aggttgtcca ctgtcatcga gaaactcaac
     5581 ggctccgcga ctgattgcac gtcgagcgcg tccagcatct cgaagctgaa cgtttccatg
     5641 aggaacttgt agtgcagtgg aatctcgtat cgggtgtggc acagaagcac cactgcctgt
     5701 cgaaatgctt ctgccacccc gagcggatcg tgttgctcgc tcgatacgtc gaatatccgg
     5761 tgcgagaccg ggagttgcac agcaacctgg tacgccgagt ccgattcgat ccggtagtcc
     5821 gtcaagaaaa cctcggcaac agcatgtctg tggacaagat gccgcggcac tgtgcgtgtg
     5881 tattccattg attgaatgca cctttcgatg ttcactcgtg tcttcggtgt cgtgtaggac
     5941 ctaatattcg acgtccgggc gcgtcatcga atcgtcaaaa ttgacgatct tttcgaaggt
     6001 cgagttcgat caggggcggc accccatctc agcgagtgtg tctggggcac ttcctgctag
     6061 aagcgtccga tccgaagttc cagtgggtgt ctgcgtgcgt cgtgaccaga gcggcggagc
     6121 atcgcgcatg cctccagctg ggaatgcacc cccagttttt gcaggatgct tcggatgtgg
     6181 gcgcgaaccg tggtcggtgc gatgtgggcg gttcgagcaa tcgagtcgac attcgagcct
     6241 ccgtagagaa ggcccacgat ctcgaactcg cgatcggtga gactgctgat tcgtgcgaat
     6301 tcttcggtgc gcaaccggat gcgttgtacg agcgaggact tgtcgtgtgc agaccgagcg
     6361 gagttggggt tgatcgcggc ctgcagctca tcgagcaggg atgtgaatgg cagttgagag
     6421 ttgagcacgg cgtgggcgcc cgctgcgagc ccccgaatca tggagttgcc gctactgaga
     6481 tcgccgacca ggaccgatcc ctcgagcgcg gagagctcct tcacctgcca atcgacgccc
     6541 ccgagtggta ggagaacgag cgctctaccg tgatcgctgg tgtgatgcct taacaggctc
     6601 atgtctgcgg tttgtggctc tgaataagcc ctggcgctga ttccggcagc gattgcattg
     6661 gcgaaccctt gatcgctatg agtggtgtac agggtgactg tgctggagag tgtcatagcg
     6721 actacatctc cggtggaggt ccactccgcc cgctctcttc gctgcgaaac caaaaccgtt
     6781 gctttcagcg tggtttcgtc agcaggttag ctatcgcagt gacctttccg ctgatgcgca
     6841 cgaaggttac ggcgatatcg cagttgcccg tcttcccatg gaaactcgcg cgttcgacac
     6901 ctgatcgtgg accttcgaat tatgctgggg cgctgaatat ttgggcgatg agtatcccgt
     6961 gtagacgagt cattgggcgt tccgtcgacg acgtgaacaa ctctccggtg cctcgaggca
     7021 ttcaggcgtc tcggtcgagt tccgcgaggc cggtgcgtcg cacctcaatg ttccgtacgc
     7081 ggccgggtcg agatattgac tgttaccgtc agttgcacat accctgttca ttggcgctga
     7141 ccttagggaa cccggcctga accggccgat cgttctcggt cactgccgac gccagaagta
     7201 gcttcgcgac gaccgacgaa acgagtttcc tcatgacaca gcatgtcgac gtactgatca
     7261 tcggcgctgg cttgtccgga atcggcgcgg cttgccacct cattcgtgag cagaccggaa
     7321 gcacttacgc gatcctcgaa cgccgcgaga acatcggtgg cacctgggac ctgttcaagt
     7381 atcccggcat ccgatcggat tccgacatgc tcaccttcgg attcggtttc cgtccgtgga
     7441 tcggaaccaa agtgctcgca gacggcgcca gtatccgtga ctacgtcgag gaaaccgcca
     7501 aggaatacgg cgtcaccgac cacatcaact tcggccgcaa ggtcgtggca atggacttcg
     7561 atcgcgcgac tgcacagtgg tccgtgaccg tcctggtcga ggcgacgggg gagaccgaga
     7621 cgtggaccgc gaacgtcctc gtcggcgcct gtggttacta caactacgac aagggatacc
     7681 gccccgcctt ccccggtgag gacgacttcc gcggtcagat cgtgcacccg cagcactggc
     7741 cggaggatct cgattacacc ggaaagaagg tagtggtcat cggttccggt gccaccgcga
     7801 tcaccctgat cccgtcgatg gcccccaccg ccggtcacgt caccatgctg cagcgctcgc
     7861 ccacgtggat ccaggcgctg ccgtccgagg accctgttgc caagggtctc aagcttgcgc
     7921 gcgttcccga ccagatcgct tacaagatcg gtcgagcccg caatatcgca ctgcaacgcg
     7981 ccagctttca gctctcacgc accaatccga agctggcgaa gaagctgttc ctcacccaga
     8041 tccgcttgca actcggcaag aacgtggacc tgcgtcactt cacccccaac tacaacccgt
     8101 gggatcagcg cctgtgcgtg gttcccaacg gggacctgtt caaggtgctc aagagcggca
     8161 aggccgacat cgtcaccgac cgtatcgcca cgttcaccga gaagggcatc gtgaccgagt
     8221 cgggccgcga aatcgaggcc gacgtcatcg tcacggcgac cggcttgaac gtacagatcc
     8281 tgggcggggc gaccatgagc atcgacggcg agcccgtcaa gctcaacgag acggtcgcct
     8341 acaagagcgt gctctactcc gacattccga acttcctgat gatcctcggc tacaccaacg
     8401 catcctggac gctcaaggcc gacctggccg cgtcctatct gtgtcgcgta ctcaagatca
     8461 tgcgtaagcg cgactacacg actttcgagg ttcacgccga acctgaggac ttcgccgaag
     8521 aatctctcat gggcggagcc ctgacctcgg gctacatcca gcgcggcgac ggagagatgc
     8581 cgcgtcaggg tgcccgcggt gcgtggaaag tggtcaacaa ttactaccgc gaccgcaagc
     8641 tgatgcacga cgccgagatc gaagacggtg tgctgcagtt cagcaaggtc gatattgctg
     8701 tcgtgcctga tagcaaggtc gccagcgcat agtcggctcc accgggggat ggaccggtcg
     8761 taccagtggg gacgtcggag aatgtgctgg agatgttgcg tcgacgtacc cgcgtcacgg
     8821 ggtttgcgat cggtgagttc gcttccgtaa tcgtcggttg tgcaccacca cgggaagaac
     8881 ataggtagcg accatgatca cggcgagcag tccgagatag agatatggcc aggtccatcc
     8941 cgctgctcgt atggccagca acgatccgcc catgatcaag ggcatcccgg ccccggcgag
     9001 caggtcgacg tcacggggcg agacgggtgg ggttccgagc agaggtacgc ggctgcttga
     9061 gttttctctg atcgtgcgca tcactgacat gcagtagagg gcggcgccgg cgatcgcgat
     9121 cgccgcgcca agtgcaactt cgacgacgcc ggttctcgcg gacatcaatg cgtaggcaaa
     9181 cacacccacc gttcccaaag tgaccggtgc cgacttccag atcggcgtcg acttatgggt
     9241 gtcggcggga acactgaccg ggcgcatcgg ttcgagaggc tccatcagct cagcgtgcca
     9301 tgcgccgctc gggaaacaaa ctgcctgtgc cgtttctggc agactccggc tgcctggcct
     9361 ggatgtcggg acccctcact acgattctcg tcgtgaccga ttacgacgat ctcgacatgt
     9421 tcggtggcct cgacgcctcc tccctgccgc tccgccagca gcagattctg atgaccatcc
     9481 gggatctcgt ggctgccaac gggtgcacgc cgagcacacg gcagatcgga gacgcggttg
     9541 gtctgcgctc gacgtcgacg gtgtccaagc acctcaagag tctcgaagag aagggcttcc
     9601 tccgtagggg agcggccatg gctcggcagt tggacgtgcg gccgttcctc gtaggcgcga
     9661 aggaacagtc gtcgtcgaac accgtgacgg ttccggtcgt cggtgacatc gcggccggcg
     9721 cgccgatcct cgcggaggag cacgccgacg aggtgcttgc gcttcctcgc gagctcgtcg
     9781 gctccggcac ggtgttcgga cttcgggtgc gtggtgagtc gatggtcgac gcagcgatct
     9841 gtgacggtga cgtcgtcgtg gtgcgtcgcc aggacgaagc tcattccggt gagatcgtcg
     9901 ccgcgatgat cgacggcgag gccacggtga aggtgctccg gcgtcgcgac ggtcacgtgt
     9961 acctcgagcc tcgcaatccc gcgtacagcg tcatcgacgg cgatcacgcg gtcatcctgg
    10021 gcaaagttgt ttccgtgatg cgccgtatct gagaggtgtc cggtctcaac gacgtctggc
    10081 gcggggaccg ccgtattcct catcggtgac tttgtcgccc catgtagttt cggtgcctcc
    10141 gtcgccgggt ccttcccaca gtgcgaggtg gctcatgaaa cggtcgggtg ccgcgccgtg
    10201 ccagtgttcc tctcccgctg gggtgtagac ggtctcaccc gggtggactt cgacgatctc
    10261 gccgccgcga gcttgaacca gcgcggtacc ggagacgatg tggagggtct gtccgactgc
    10321 atgggagtgc cagtcggttc gcgcgcacgg tgcaaagcga accatgttgg cgcgcatcct
    10381 cgacggctcc tgtccggcgt agatgacgtc ccaccagacg tcgccggtga accagtcgct
    10441 gggtgctttg ctggtgggct ggggcttcaa gatttccatg aggtctcctc ggaatcgaaa
    10501 ggggcggttc agttctgcgt cagcggaaca agtcgaatgg tgttgggtgc ggcggcgcgg
    10561 ccggtgacgg agccgacgat ctgcgggccg tagcggtcgt atttggtgtg ataggcggag
    10621 tcgatcgacg agtgggcatc gggtgcggcg gactcgaacg ccacgtcccg ctcgattcct
    10681 cctgctcgga ttcggcccgt tccacttgcc gttgctcgga cgaaccacgg gttgtcggtt
    10741 ccgtaggccg agcgaacgta cagttggttg tcggtacgga caacccaaat cgtgacgtac
    10801 ggccttgcgg ttccgtctcg gcggtacgag gagacctgca actcctcggc gtcgccgatc
    10861 ttggtcagtt cagcctcggt ccacgagt
//
