LOCUS       NZ_CP114040            10897 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Nannocystis poenicansa strain FL3 chromosome, complete genome.
ACCESSION   NZ_CP114040
VERSION     NZ_CP114040.1
KEYWORDS    RefSeq.
SOURCE      Nannocystis poenicansa
  ORGANISM  Nannocystis poenicansa
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Minimal conservation of predation-associated metabolite biosynthetic
            gene clusters underscores biosynthetic potential of Myxococcota
            including descriptions for ten novel species: Archangium lansinium
            sp. nov., Myxococcus landrumus sp. nov., Nannocystis bai
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2022) Biomolecular Sciences, University of
            Mississippi, Faser Hall 407, Oxford, MS 38655, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: IPA v. 10.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: PacBio Sequel IIe
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026965555.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 12:45:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,229
            CDSs (total)                      :: 10,129
            Genes (coding)                    :: 10,068
            CDSs (with protein)               :: 10,068
            Genes (RNA)                       :: 100
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 9 of 61
            Pseudo Genes (incomplete)         :: 52 of 61
            Pseudo Genes (internal stop)      :: 2 of 61
            Pseudo Genes (multiple problems)  :: 2 of 61
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:28:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 11718852
            Orig. end                         :: 11729749
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10897
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="40"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10897
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10897
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5897](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5897)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    18..27
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(462..475)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.18"
                     /tool="antismash"
     gene            948..2543
                     /locus_tag="O0S08_RS44950"
                     /old_locus_tag="O0S08_44950"
     CDS             948..2543
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775563.1"
                     /locus_tag="O0S08_RS44950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_44950"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_269035677.1"
                     /transl_table=11
                     /translation="MTSAPQRVRVDLHPDALLDPDALRRAGARAAGLREADVRHVAVAH
                     RSIDARRGRVRVHVELLVHVDKDMPEEPCYLPHVLPNMSQEPAVVIVGAGPAGMFCAWG
                     LARAGVRAVVLERGREVRARRHDLAGLSQRGELDPESNYCFGEGGAGTFSDGKLYTRAT
                     KRGPVDLVLRAFAGYGAPPEVLVDARPHIGTNRLPRVITAMREHLIGAGVAFRFEARAD
                     ALVVQGGAAAGVRLRGGEVVPARAVVVAPGHSARDVQAWLRAAGVKLSFKPFALGVRIE
                     HPQALIDRLQFGALAGHPALGAASYRLVEQTAAGAAFSFCMCPGGYIVPAATDPGMQVV
                     NGMSPHSRRGRFANSGLVTEVGPQHLAAAGLSPDDPLAGVDYQARFEAAAYVAGGGAFV
                     APAQRLADLVAGAASRELPPTSYHRGLQPARLDRLLGPLAPPLQSALQALGARMPGFLG
                     DDAIAVGVESRTSCPVRIDRDGETLESPSTEHLYPCGEGAGYAGGIVSAALDGLRVAVA
                     IARRLGAPHERITS"
     misc_feature    complement(2577..2592)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.69"
                     /tool="antismash"
     gene            2674..3843
                     /locus_tag="O0S08_RS44955"
                     /old_locus_tag="O0S08_44955"
     CDS             2674..3843
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS44955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_44955"
                     /product="Ig-like domain-containing protein"
                     /protein_id="WP_269035678.1"
                     /transl_table=11
                     /translation="MVLLTACTVYQVPNESETDTTVATGDATGMTTTGTPTGTTTDEPD
                     PTTSSTSSTGTGPDPSAGPSSDPTVSSDPTTEEPPPADCDYPTSIQPIFDQRCSCHQSM
                     MPPKGLALNEGTSFAALVGVDSMGQPGTPYVDPGNSAGSWLVTKIKPMPPVGLQMPEGG
                     MLDADQVALIETWIDAGAPEAGAFDCGGGSSGEAGSVTIDEQGPVNVEIGEMLDLDVTV
                     LDEFGDPVMGATVKWLSSDETVLYVDAKGTLLGLNPGTSEVTAEVEGAVSSPLTVSVLN
                     NQAVAAATFTDALNTLSTNCGCHKGAMPPAGLAFDLAAPDVHAALLAPATQDAQTKRVI
                     PDLPGQSYLFKKLTRTTQTTGEQMPKGKAPLEAEKVQILFRWIFNGAPA"
     gene            3944..4183
                     /locus_tag="O0S08_RS44960"
                     /old_locus_tag="O0S08_44960"
     CDS             3944..4183
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012784.5"
                     /locus_tag="O0S08_RS44960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_44960"
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="WP_269035679.1"
                     /transl_table=11
                     /translation="MSADFVDLAGERVLARVTRVEHYGVYLRHGDAAILVLIVDVAREP
                     IADLRARVHVGDEVEVRILRYVHQYKLFKGSFLA"
     gene            complement(4210..5004)
                     /locus_tag="O0S08_RS44965"
                     /old_locus_tag="O0S08_44965"
     CDS             complement(4210..5004)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021364.5"
                     /locus_tag="O0S08_RS44965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_44965"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_269035680.1"
                     /transl_table=11
                     /translation="MSEEALIRAFAASVIELDAPEAVKADVRGFFARHGVAPELAAGLP
                     EAAAERLAIYRTMVHSRLRAAVVDFLPKTAARLGRPRLRREIEQFVAEAAPSAPYFWRV
                     TGEFLVWAQPRWAVDATLPDFLLDLALHEWSDAEIGNDPAGGEAASGKPLALDQPVQFD
                     GTVRLRRYGWAVHRATETEPPPPEPTALLLYRDRTEHLVKTLELTPRAAAVTSRLLAGE
                     PLQAALMGACAELGVALDDEFLAAMAGYFADLAERGALLGAA"
     gene            complement(5001..5897)
                     /locus_tag="O0S08_RS44970"
                     /old_locus_tag="O0S08_44970"
     CDS             complement(5001..5897)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238681.1"
                     /locus_tag="O0S08_RS44970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_44970"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_269035681.1"
                     /sec_met_domain="DUF692 (E-value: 4.3e-95, bitscore: 317.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSFRAGIAARAAVPTGVGLGLRSTFLAEVDRGAADGRVPFWEISP
                     DNYVARGGSRPARLARVAERFPILSHGLSLSLGGLDPLDADFLSGLHGFFEQLPPPWHS
                     DHLCFSGHDGKMLHDLLPLPWTTASAKRVAARVREVSQRLARPMLVENISYYLSLGSTP
                     LDEAAFVVEVLERADCGLLLDVNNVFVNAANHGFDPLEWLAQIPLDRVVQLHIAGHEFW
                     DDERRDLIVDTHGAPVRSEVDALLSWVVERTGALPVLLERDENIPPLQELLAERDRVDS
                     VYQAALARHAAHQGDPA"
     gene            complement(6004..6276)
                     /locus_tag="O0S08_RS44975"
                     /old_locus_tag="O0S08_44975"
     CDS             complement(6004..6276)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS44975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_44975"
                     /product="hypothetical protein"
                     /protein_id="WP_269035682.1"
                     /transl_table=11
                     /translation="MNVKHIAATLAILGSASFAVTGCKKNAEGTEVPAEGEKKADGSCG
                     ANKDGSCGANKDGSCSAKKEGEGDAAAGGETPPADGAAATPPAAN"
     misc_feature    complement(6327..6340)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.67"
                     /tool="antismash"
     gene            complement(6337..7803)
                     /locus_tag="O0S08_RS44980"
                     /old_locus_tag="O0S08_44980"
     CDS             complement(6337..7803)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS44980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_44980"
                     /product="hypothetical protein"
                     /protein_id="WP_269035683.1"
                     /transl_table=11
                     /translation="MFQRRLRARARAALATLLGLAGCAHGSARGQDGALRWRYRVEVPA
                     ALDRLRVEVCFPDGAPRRLKIDEDDAEAVAAVSEVAADGGSARLDREAAAIVLGPLPAG
                     ACVRYSVAIESLLEGSGSRLHARVGEVVSVDPRLVLWRPATLYYEADVALELSLAEGVS
                     ASVPWPATGEHRYRLPVTSLMWASQAVFGGLTHQTVEAAGATFSVAIVDQPRKLSEAGV
                     SEWITTAAETVATLYGRFPTERMQVVVLPFPGGGGPVYFGMALRGGGPSVQLLVSSEAA
                     DHAFPGEWVAIHEFLHHGMPFVQQADAWLSEGFVTYYTEILPTRRGFRSESAGWQALLD
                     GFGRGRKDGAGQPLAQASREMHDNHSYQRVYWSGAALALLADVSLRLGVGERSLDAAMR
                     HLLRCCAKTPRVWTAEAALRELDAWAGSPVFTELAASWLPRAEFPELGPVYRKLGLDVI
                     KGEVQIHPSAPAAAVRRAIFARPSGRSESL"
     gene            7828..8427
                     /locus_tag="O0S08_RS44985"
                     /old_locus_tag="O0S08_44985"
     CDS             7828..8427
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF020398.5"
                     /locus_tag="O0S08_RS44985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_44985"
                     /product="DUF1802 family protein"
                     /protein_id="WP_269035684.1"
                     /transl_table=11
                     /translation="MRVTSPVALKEWAALCAGLDAGEQSVLVRKGGIREPRAAVGQAPT
                     FSLEHRGFWLLPTWFHAREPGRDRDLAPAARARLPQVLADAPPPGRLHFRLWAEVDAAV
                     EVRDLARLDRLGDAHGLGRECVADRFAYRKPGLWVLFLRTYRSGQLLDLPDRAEYAGCH
                     SWVDLAEELAGAVEPVLTDREHGARVAELRSALGDG"
     gene            8424..9755
                     /locus_tag="O0S08_RS44990"
                     /old_locus_tag="O0S08_44990"
     CDS             8424..9755
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.5"
                     /locus_tag="O0S08_RS44990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_44990"
                     /product="MFS transporter"
                     /protein_id="WP_269035685.1"
                     /transl_table=11
                     /translation="MTDPSTTWRGRAAAILIVLTVINFLNYIDQMLLSSVLEQIKLEFT
                     LSDTAGGLLGSLFVITYTIAAPLGYLGDRFKRKNIIAGGVALWSVATLAAAFARSYEEL
                     LVCRALLGIGEAAYATTAPSIIADLYGPDERSRKLALFYVATPVGSALGFILGGLIGEH
                     FGWRAVFLVGGLPGLVFAALAARIVEPERGRLDPASTRTEGTFFGALGRIVRTPAWGIN
                     TVGLAMMTFTVGGLSLWMPTYLERVHGMSTGAAGPVFGAVTVLAGLCGTLLGGSLGDRL
                     QARSAGGYFWLSGVGLVLSAPLIVLLTRMDDLVALFVVAFFAELLLFLNTGPLNAALVG
                     CVPAWLRGTAFAVNIFCIHAFGDALSRTLIGYVSDRAKAAYLAGQVPTESAVRAAEAAG
                     LNLAIATTAIPVLLGGLVLLWGARRIARAPEGLMTYAGGPHRHR"
     misc_feature    complement(10609..10624)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.03"
                     /tool="antismash"
ORIGIN
        1 gtcgtgcaga tcaagtggaa ggcggccgac aagctgctgc gcgtcggcaa gggcaagcag
       61 aagatctccg cggtgcaggc cgcgatcgac accaagctcg cgccgcaatc aggggacatc
      121 cctggagtcg agctcaggct gtcggcggtg gtcgagccgg tccaggtgca ggtccccaac
      181 gtgctggcga tggtccccgg cggcgacctg cgcgacgagc tcgtggtcct cggcgcgcac
      241 tacgaccaca tcggcgccgt cggccgcggc gagtgcggcg agagccgcaa gggcgacgcg
      301 gtcgacgtcg tctgcaacgg cgccgacgac aacgccagcg gcacggcgat gctgctcgag
      361 ctggcgcggc acttctcgca gcagcggccg cggcgcacgg tggtgttcgc ccacttcgcc
      421 ggcgaggagc tgggcctcct cggctccaag gcgctggcgg acaagccgcc gtttgcgctc
      481 gacaaggtcg tcgccatggt caacctcgac atgatcggcc gactcggacc gaagggcctg
      541 gcgatcggcg gcatcgtctc ctcggacgcc tggatgccgc tgctcgacaa ggtcggcacc
      601 gccggcctgt cggtcctcta cgaggccagc gtcgccaccc gcagcgacca cgccagcttc
      661 tacaagaaga acctgccggt gctgttcttc ttcaccggca cccacgccga ctaccaccgg
      721 ccgggcgacc acgccgacaa gatcaactac gacggtctga tggcgatcgg ctcgctcgtc
      781 cacggggtcg cgcgggcggt cgccgacggc ctcccggtga cctacaaggc cccgcccgag
      841 ggcggcggcc tcgcctccgg cttgcccgga tcgaaccccg acaccgtcgt caagcgggtc
      901 cagggcacct cggccccggt caccgccccc gccgcggagt gagtgccgtg acctctgcgc
      961 cgcagcgggt gcgcgtcgac ctccaccccg acgccctcct cgaccccgac gccctccgcc
     1021 gcgccggcgc ccgcgcggcc ggcctgcgcg aggccgacgt ccgccacgtc gccgtcgccc
     1081 accgctcgat cgacgcccgc cgcggccgcg tccgcgtcca cgtcgagctg ctcgtgcatg
     1141 tcgacaagga catgcctgaa gagccatgtt atttgccaca tgtcctgccg aacatgtccc
     1201 aagagcctgc cgtggtgatc gtcggcgccg ggccggcggg gatgttctgc gcctggggcc
     1261 tggcgcgcgc cggggtgcgg gcggtcgtgc tcgagcgcgg gcgcgaggtg cgggcgcggc
     1321 ggcacgacct cgccggcctc agccagcgcg gcgagctcga ccccgagagc aactactgct
     1381 tcggcgaggg cggcgcgggc accttctccg acggcaagct gtacacgcgg gcgaccaagc
     1441 gcggcccggt cgacctggtc ctgcgggcgt tcgccggcta cggcgcgccg ccggaggtcc
     1501 tcgtcgacgc ccgcccgcac atcggcacca accgcctgcc gcgggtgatc accgccatgc
     1561 gcgagcacct gatcggcgcc ggcgtggcgt tccgcttcga ggcccgcgcc gacgccctgg
     1621 tcgtgcaggg cggcgcggcc gcgggcgtgc gcctgcgcgg cggcgaggtc gtgccggcgc
     1681 gcgcagtggt ggtcgcgccc ggccactcgg cccgcgacgt ccaggcctgg ctgcgcgcgg
     1741 ccggggtcaa gctgtcgttc aagccgttcg cgctgggcgt ccgcatcgag cacccgcagg
     1801 cgctcatcga ccgcctgcag ttcggcgcgc tcgccggcca cccggccctc ggcgcggcct
     1861 cgtaccgcct ggtcgagcag accgccgccg gcgcggcctt ctcgttctgc atgtgccccg
     1921 gcggctacat cgtccccgcg gccaccgacc ccggcatgca ggtcgtcaac ggcatgagcc
     1981 cgcacagccg ccgcggtcgg ttcgccaact cgggcctcgt caccgaggtc ggcccgcagc
     2041 acctcgcggc cgccggcctg tcgcccgacg atccgctcgc cggcgtcgac taccaggccc
     2101 gcttcgaggc cgcggcctac gtcgccggcg gcggggcctt cgtcgccccg gcgcagcgcc
     2161 tggccgacct ggtcgcgggc gccgcctcgc gcgagctgcc gcccacgagt tatcaccgcg
     2221 gcctgcagcc ggcccgcctc gaccgcttgc tcggcccgct cgcgccgccg ctccaaagcg
     2281 cgctgcaggc cctcggcgcg cgcatgccgg gcttcctcgg cgacgacgcg atcgccgtgg
     2341 gtgtcgagag ccgcaccagc tgcccggtcc gcatcgatcg cgacggcgag acgctcgagt
     2401 cgccgagcac cgagcacctc tacccgtgcg gcgagggggc gggttacgcc ggcggcatcg
     2461 tctccgccgc gctcgacggc ctccgcgtcg cagtagcgat cgcgcgccgc ctcggcgcgc
     2521 cgcacgagcg aattacgagc tgaccttcag gacaggtcgc tcgcgtctct cgttcgcgtg
     2581 atgtttttca ctcctacacc cggcgaggga ggactgtctt cacgcctgcg catgcggttg
     2641 catgtgcgca tgcgctcgac acgatgtctt cccttggtcc tgctgaccgc gtgcaccgtc
     2701 tatcaggtcc ccaacgagag tgagaccgac accaccgtgg cgaccggcga cgccacgggg
     2761 atgaccacca cgggcacgcc gaccggcacc accaccgacg agcccgaccc gacgacctcg
     2821 tcgacgagca gcaccggcac cgggcccgac ccgagcgccg ggcccagcag cgatccgacg
     2881 gtgagcagcg atccgaccac cgaggagccg cccccggcgg actgcgacta cccgacgtcg
     2941 atccagccga tcttcgacca gcggtgcagc tgccaccaga gcatgatgcc gcccaagggg
     3001 ttggccctga acgagggcac gtcgttcgcc gccctggtcg gcgtcgacag catgggccag
     3061 ccgggcaccc cctacgtcga cccgggcaac tccgccggca gctggctggt cacgaagatc
     3121 aagccgatgc cgccggtcgg cctgcagatg cccgagggcg gcatgctgga cgccgaccag
     3181 gtcgccctga tcgagacgtg gatcgacgcc ggcgcgcccg aggccggcgc gttcgactgc
     3241 ggcggcggca gcagcggcga ggccggctcg gtcacgatcg acgagcaagg cccggtgaac
     3301 gtggagatcg gcgagatgct cgacctcgac gtcactgtcc tcgacgagtt cggcgacccg
     3361 gtcatgggcg cgacggtcaa gtggctgtcg agcgacgaga cggtcctcta cgtcgacgcc
     3421 aagggcaccc tgctcggcct caaccccggc acctccgagg tcaccgccga ggtcgagggc
     3481 gcggtcagct cgccgctcac cgtctcggtc ctcaacaacc aggcggtcgc cgcggccacc
     3541 ttcaccgacg cgctcaacac gctctccacc aattgcggct gccacaaggg cgcgatgccc
     3601 cccgccggcc tggccttcga cctcgccgcc cccgacgtcc acgccgccct gctcgcgccc
     3661 gcgacccagg acgcccagac caagcgcgtc atccccgacc tccccggcca gagctacctg
     3721 ttcaagaagc tgacccgcac cacgcagacc accggcgagc agatgcccaa gggcaaggcc
     3781 ccgctcgagg ccgagaaggt gcagatcctg ttccgctgga tcttcaacgg cgcgccggcg
     3841 tgacgcgagc agaccttcgg gacatgtctc gaaagacgtg tcccgaacga cagggcggat
     3901 cgccgctcga ggcgggcccc ggagtccacg ggtcgaggcc ggcatgagcg ccgacttcgt
     3961 cgacctcgcc ggcgagcggg tactcgcccg cgtcacccgc gtggagcact acggcgtcta
     4021 tctgcgacac ggagacgccg cgatcctggt cctgatcgtg gacgtggcgc gcgagcccat
     4081 cgccgatctg cgggcccggg ttcacgtcgg ggacgaggtc gaggtccgga tcctccggta
     4141 cgtccaccag tacaagctgt tcaagggcag cttcctggcc tgacgggatg acctgtccgc
     4201 gaggacagct caggccgcgc cgagcagcgc cccgcgctcg gcgaggtcgg cgaagtagcc
     4261 ggccatcgcc gccaggaact cgtcgtcgag cgccacgccg agctcggcgc acgcgcccat
     4321 caaagcggct tgcagcggct cgcccgccag caggcggctc gtcaccgccg cagcgcgggg
     4381 cgtcagctcc aacgtcttga ccaggtgctc cgtgcggtcg cggtagagga gcagcgccgt
     4441 cggctccggc ggcggcggct ccgtctcggt cgcgcggtgg accgcccagc cgtagcggcg
     4501 gaggcggaca gtgccgtcga actgcaccgg ctggtcgagc gccagcggct tgccgctcgc
     4561 ggcctcacct ccggccgggt cgttgccgat ctcggcatcg gaccactcgt gcagcgccag
     4621 gtcgagcagg aagtccggca gcgtcgcgtc gaccgcccac cgcggctgcg cccagaccag
     4681 gaactcgccc gtcacccgcc agaagtacgg ggccgacggc gccgcctcgg ccacgaattg
     4741 ctcgatctcc cggcgcagcc gcggccggcc gaggcgcgcc gcagtcttcg gcaagaagtc
     4801 gaccaccgcc gcccgcagcc ggctgtggac catcgtgcgg tagatcgcca ggcgctcggc
     4861 cgccgcttcc ggcaagccgg ccgccagctc cggcgccacc ccgtggcggg cgaagaaccc
     4921 gcggacgtcg gccttcaccg cctcgggcgc gtcgagctcg atcacgctgg cggcgaacgc
     4981 ccggatcagc gcctcctcgc tcatgccggg tctccctggt gcgcagcgtg gcgggccagc
     5041 gcggcctggt acaccgagtc gacgcggtcg cgctcggcca gcagctcctg cagcggcggg
     5101 atgttctcgt cgcgctcgag cagcaccggc agcgcgcctg tcctttcgac cacccacgac
     5161 agcagcgcgt cgacctcgct gcggaccggc gcgccgtggg tgtcgacgat caggtcgcgg
     5221 cgctcgtcgt cccagaactc gtggccggcg atgtgcagct ggaccacgcg gtcgagcggg
     5281 atctgggcca gccactcgag cgggtcgaag ccgtggttgg ccgcgttgac gaacacgttg
     5341 ttgacgtcga gcagcagccc gcagtcggcc cgctcgagca cctcgaccac gaaggccgcc
     5401 tcgtcgagcg gcgtcgaccc gagcgacagg tagtagctga tgttctcgac cagcatcggc
     5461 cgcgccagcc gctgcgacac ctcgcgcacg cgggccgcca cgcgcttcgc cgacgccgtc
     5521 gtccacggca gcggcagcag gtcgtgcagc atcttgccgt cgtggccgga gaagcacagg
     5581 tggtcggagt gccacggcgg cggcagctgc tcgaagaacc cgtgcaggcc gctcaggaag
     5641 tcggcgtcga gcgggtcgag cccgccgagc gacagcgaca ggccgtgcga gaggatcggg
     5701 aagcgctcgg cgacgcgggc caggcgggcc gggcggctgc cgccgcgggc cacgtagttg
     5761 tccggcgaga tctcccagaa cggcacgcga ccgtcggcgg ccccgcgatc gacctcggcg
     5821 aggaacgtgc tgcgcaggcc gaggccgacc ccggtgggaa ccgcggcgcg cgcggcgatg
     5881 cctgctcgaa aagacatgtc cgaagaaaca gtatagcagg ccacccgaaa tgcgcccggc
     5941 ccgggctcgg cgccgcgcag gcggggcctg cggccgaatc cgggccggtc gggggcgcgg
     6001 ggctcagttg gcagccggcg gggtcgcggc cgcgccgtcg gccggcgggg tctcgccacc
     6061 ggcggcggcg tcgccctcgc cttccttctt ggcgctgcag ctgccgtcct tgttcgcgcc
     6121 gcagctgccg tccttgttcg cgccgcagct gccgtcggcc ttcttctcgc cctccgcggg
     6181 gacctcggtg ccctccgcgt tcttcttgca gccggtgacg gcgaacgagg cggagccgag
     6241 gatggcgagg gtggcggcga tgtgcttgac gttcatgtgg agtgcttcct ttttgctcgg
     6301 gtgtgggggc acccgcgagt cgcggatcga tctggctcag aggctttcgg agcgaccgga
     6361 gggtctggcg aaaatggccc gacggacggc ggctgcgggg gcggatgggt ggatctgcac
     6421 ttcccccttt atcacatcga ggccgagctt cctatagaca gggccgagct cggggaactc
     6481 ggcgcgcggc aaccaggacg ctgcgagctc ggtgaacacg gggctgccgg cccaggcgtc
     6541 gagctcgcgg agggccgcct cggcggtcca cacccgcggc gtcttggcgc agcagcgcag
     6601 caagtggcgc atggcggcgt cgagcgatct ctcgccgacg ccgagcctca ggctgacgtc
     6661 ggcgagcaag gcgagcgccg cgccggacca gtagacgcgc tgataactgt ggttgtcgtg
     6721 catctcgcgg ctcgcttgcg cgagcggctg gccggcgccg tccttgcgcc cgcggccgaa
     6781 cccgtcgagc agcgcttgcc agccggccga ctcgctgcgg aagccgcggc gggtcggcaa
     6841 gatctccgtg tagtaggtga cgaagccctc cgacagccac gcgtcggcct gctgcacgaa
     6901 cggcatgccg tggtggagga actcgtggat cgccacccac tcgccgggga aggcgtggtc
     6961 ggccgcctcg ctcgacacga gcagctgcac cgacggtcct ccgccgcgca gcgccatccc
     7021 gaagtatacc gggccgccgc cgccggggaa cggcagcacc accacctgca tgcgctcggt
     7081 cgggaaccgc ccgtagagcg tcgccaccgt ctcggccgcg gtcgtgatcc actcgctcac
     7141 gccggcctcg ctgagcttgc gcggctggtc gacgatcgcc accgagaacg tcgcgcccgc
     7201 ggcctcgacc gtctggtggg tcagcccgcc gaacaccgcc tgcgacgccc acatcagcga
     7261 ggtcaccggc aggcggtatc gatgctcgcc ggtcgcgggc cacggcaccg acgccgacac
     7321 gccctcggcc agcgacagct cgagcgccac gtcggcctcg tagtagagcg tcgccgggcg
     7381 ccacagcacc agccgcgggt cgaccgacac cacctcgccg acgcgcgcgt gcaggcgcga
     7441 acccgagccc tccagcagcg actcgatcgc caccgagtag cgcacgcagg cgccggcggg
     7501 cagcggcccg agcacgatcg ccgccgcctc gcggtcgagc cgcgccgagc cgccgtcggc
     7561 cgccacctcc gacaccgccg ccaccgcctc cgcgtcgtcc tcgtcgatct tgaggcgccg
     7621 cggcgcgccg tcggggaagc agacctcgac gcgcagacgg tcgagcgccg ccggcacctc
     7681 gacgcggtag cgccagcgca gcgccccgtc ctgcccgcgc gcggacccgt gggcgcagcc
     7741 cgcgagcccc agcagggtcg ccagcgcggc cctcgcccgc gcacgcagtc ggcgctggaa
     7801 caccgcccca tggtagcctg agggcccatg cgggtcacat cccccgtcgc cctcaaggag
     7861 tgggcagccc tgtgcgccgg cctcgacgcc ggcgaacaga gcgtcctggt gcgcaagggc
     7921 gggatccgcg agccccgcgc ggccgtcggc caggccccga ccttcagcct cgagcaccgc
     7981 ggcttctggc tgctccccac ctggttccac gcgcgcgagc ccgggcgcga ccgcgacctc
     8041 gccccggccg cgcgcgcacg cctgccgcag gtcctggccg acgccccgcc acccggccgc
     8101 ctgcacttcc gcctgtgggc cgaggtcgac gccgccgtcg aggtccgcga cctcgcgcgc
     8161 ctcgatcgtc tcggcgacgc ccacggcctc ggccgcgagt gcgtcgccga ccgcttcgcc
     8221 taccgcaagc ctggcctttg ggtcctgttc cttcggacct atcgctcggg acagctgctc
     8281 gacctgcccg accgggccga gtacgccggc tgtcactcgt gggtcgacct cgcggaggag
     8341 ctcgcgggcg ccgtcgagcc ggtgttgacc gaccgcgagc acggggcgcg cgtggcggag
     8401 ctgcggtccg cgctggggga cggatgacag acccatccac gacgtggcgc ggccgggcgg
     8461 cggcgatcct gatcgtcctc acggtcatca acttcctcaa ctacatcgac cagatgctgc
     8521 tcagcagcgt gctcgagcag atcaagctcg agttcaccct gagcgacacc gcgggcggcc
     8581 tgctcggcag cctgttcgtc atcacctaca cgatcgccgc gccgctcggg tacctcggcg
     8641 accgcttcaa gcgcaagaac atcatcgccg gcggcgtcgc cctgtggagc gtcgccactc
     8701 tggccgccgc gttcgcgcgc agctacgagg agctgctggt ctgtcgcgcc ctgctcggca
     8761 tcggcgaggc cgcctacgcg accaccgccc cgtcgatcat cgccgacctc tacggccccg
     8821 acgagcgcag ccgcaagctc gcgctgttct acgtcgccac cccggtcggc tcggcgctgg
     8881 gcttcatcct cggcggcctt atcggcgagc acttcggctg gcgcgcagtg tttttggtcg
     8941 gcggtctgcc ggggctcgtg ttcgccgcgc tggccgcgag gatcgtcgag ccggagcgcg
     9001 ggcggctcga cccggcgagc acgcggaccg agggcacctt cttcggcgcg ctggggcgga
     9061 tcgtccgcac cccggcgtgg gggatcaaca ccgtcggcct ggcgatgatg accttcaccg
     9121 tcggcggcct gtcgctgtgg atgccgacct acctcgagcg cgtgcacggc atgagcaccg
     9181 gcgccgcggg cccggtgttc ggcgcggtca ccgtcctcgc cggcctgtgc gggaccctcc
     9241 tcggcggcag cctcggcgac cgtctgcagg cccgttccgc gggcggctac ttctggttga
     9301 gcggcgtggg gctggtgctg agcgcgccgc tgatcgtcct gctcaccagg atggacgacc
     9361 tcgtcgcgct gttcgtggtc gccttcttcg ccgagctgct gctgttcctc aacaccggcc
     9421 cgctgaacgc cgcgctggtc gggtgcgtgc cggcgtggct gcgcggcacc gcgttcgcgg
     9481 tcaacatctt ctgcattcac gcgttcggcg acgcgttgtc gcgcaccttg atcggctacg
     9541 tcagcgaccg ggccaaggcc gcctacctcg ccggtcaggt gccgaccgag tccgccgtgc
     9601 gggcggcgga ggcggcgggc ctcaacctcg cgatcgccac caccgcgatt cccgtgctgc
     9661 tcgggggatt ggtcctcctg tggggcgcgc gccggatcgc ccgcgccccc gaggggctga
     9721 tgacctacgc gggcggcccg catcggcacc gttgaggacg ggcgtcccgg tggacgcgcc
     9781 cccgtcggct ctcgtatcgt gtcggcaatt ctgcgcgccc atcgccgggc gccttcgctt
     9841 aggagctcct tccgatgatc acccgcaacg ccgcggccct gctgttctcc tgcatcgcgc
     9901 tggccggctg caccttcgac ggcaccggct tcggcgagca gcccccgacg acccaggcgg
     9961 acaccgagac caccacgggc acgtcgacgt ccaccaccac gatggacgtc accaccgagc
    10021 cgacgaccac cgtcgacccc ggcgacctgt gcggcaacta tcagctggac gacggcgagg
    10081 agtgcgacca gggcccgaag ggcaacggcg tgtgcgcgcc gacctgcgtg tacaacgtct
    10141 gcggcgacgg ctacgtcggc gccggcgagt cctgcgacga ccagaccgag ggctgcgtca
    10201 attgcaagct ggcctcgtgc agcaacggca cgatcgacac cggcgaggag tgcgacgacg
    10261 gcaacctcga cgagaccgac gcctgcctca ccacctgcaa gaacgcgacc tgcggcgaca
    10321 gcttcgtcca gaacggggtc gaggcctgcg acgacggcaa cgacctcgac accgacgcct
    10381 gcgtcgccgg ctgcaagatg gcgagctgcg gcgacggcct gaagtgggag ggcaacgagt
    10441 tctgcgacga cggcaaccag gtcgacaacg acgagtgccc caacagctgc ggcccgcccg
    10501 gctgcggcga cggcatcgtc tcgcccggcg aagagtgcga cgacggcaac gtggtcaacg
    10561 aggacggctg caccaacggg tgcaagaacc cggtgtgcgg cgacagcatc gaacaggccg
    10621 gcgaggagtg cgacaacggc gtgacccaga acgccgacaa cgccgcctgc acgctcatgt
    10681 gcaaggacgc cgtgtgcggc gacaccaagg tccacatggg cgtcgagcag tgcgacgacg
    10741 gcaacatggt cgacgaggac ggctgcacca acatgtgcaa gaacccggcc tgcggcgacg
    10801 gcatcaagaa cggcacggag gagtgcgacg acgccaacac ggacgagaac gacgcctgcc
    10861 tcaacgactg caagagcaac gtctgcggcg acggcaa
//
