LOCUS       NZ_CP114040            11872 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Nannocystis poenicansa strain FL3 chromosome, complete genome.
ACCESSION   NZ_CP114040
VERSION     NZ_CP114040.1
KEYWORDS    RefSeq.
SOURCE      Nannocystis poenicansa
  ORGANISM  Nannocystis poenicansa
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Minimal conservation of predation-associated metabolite biosynthetic
            gene clusters underscores biosynthetic potential of Myxococcota
            including descriptions for ten novel species: Archangium lansinium
            sp. nov., Myxococcus landrumus sp. nov., Nannocystis bai
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2022) Biomolecular Sciences, University of
            Mississippi, Faser Hall 407, Oxford, MS 38655, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: IPA v. 10.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: PacBio Sequel IIe
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026965555.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 12:45:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,229
            CDSs (total)                      :: 10,129
            Genes (coding)                    :: 10,068
            CDSs (with protein)               :: 10,068
            Genes (RNA)                       :: 100
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 9 of 61
            Pseudo Genes (incomplete)         :: 52 of 61
            Pseudo Genes (internal stop)      :: 2 of 61
            Pseudo Genes (multiple problems)  :: 2 of 61
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:28:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 11164641
            Orig. end                         :: 11176513
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11872
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="36"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11872
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11872
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6872](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6872)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1135..1148)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.47"
                     /tool="antismash"
     misc_feature    2326..2340
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: medium, score: 23.69"
                     /tool="antismash"
     misc_feature    complement(2842..2857)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.14"
                     /tool="antismash"
     misc_feature    2848..2868
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 23.13"
                     /tool="antismash"
     gene            2859..3323
                     /locus_tag="O0S08_RS42735"
                     /old_locus_tag="O0S08_42735"
     CDS             2859..3323
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /locus_tag="O0S08_RS42735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_42735"
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="WP_269035289.1"
                     /transl_table=11
                     /translation="MTTTRTSWTLALSLATLVALPSCKKEATTPPDGSAGGDAAAGSND
                     GGGGNGGNEGASEEPLTADSFEEAMQARQGDVADCFAQAKEAKPDLAGKLALDVTVAGD
                     GSVSSIKFTDTSTIKEASITSCIEEKAKGWKFNKPSDGNPMTLGYSLNLS"
     gene            complement(3413..4276)
                     /locus_tag="O0S08_RS42740"
                     /old_locus_tag="O0S08_42740"
     CDS             complement(3413..4276)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (51-202). E-value:
                     1.9e-18. Score: 58.4. Matches aSDomain:
                     nrpspksdomains_O0S08_RS42740_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.5"
                     /locus_tag="O0S08_RS42740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_42740"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_269035290.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.3e-14, bitscore:
                     52.4, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 3.2e-16, bitscore:
                     58.4, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPRAVVTGAAGTVGSRVCDRLAAAGWGVRGLVRSPALPGAPVEL
                     VQADLGDADALRVACRGADVVVHCAAALSSDRRMCRETNVEGTRHLLAALGASRRARFV
                     FVSSVSVYDVRHGWSVDEQSPQWSDDVYPYGRSKVEAEELVRSDDHGLAWVVLRPVPVL
                     SMHRSSFWGPLALERARRSAQPQFASRTMPFVHVDNLADAALLAATHPRAVGGVFDVVD
                     GHGETRAYHEALAAATGRRPADPPDDAPAMRVAGTRIRSELGYSPRDRFAEFLGELARA
                     TVTPES"
     aSDomain        complement(3671..4123)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O0S08_RS42740_NAD_binding_4.1"
                     /evalue="1.90E-18"
                     /label="O0S08_RS42740_NAD_binding_4.1"
                     /locus_tag="O0S08_RS42740"
                     /protein_end="202"
                     /protein_start="51"
                     /score="58.4"
                     /tool="antismash"
                     /translation="DADALRVACRGADVVVHCAAALSSDRRMCRETNVEGTRHLLAALG
                     ASRRARFVFVSSVSVYDVRHGWSVDEQSPQWSDDVYPYGRSKVEAEELVRSDDHGLAWV
                     VLRPVPVLSMHRSSFWGPLALERARRSAQPQFASRTMPFVHVDNLAD"
     CDS_motif       complement(4199..4261)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O0S08_RS42740_0001"
                     /evalue="2.80E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="O0S08_RS42740"
                     /protein_end="26"
                     /protein_start="5"
                     /score="10.4"
                     /tool="antismash"
                     /translation="VVTGAAGTVGSRVCDRLAAAG"
     gene            complement(4273..5001)
                     /locus_tag="O0S08_RS42745"
                     /old_locus_tag="O0S08_42745"
     CDS             complement(4273..5001)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS42745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_42745"
                     /product="hypothetical protein"
                     /protein_id="WP_269035291.1"
                     /transl_table=11
                     /translation="MRRDVIVVDDFYADPAAVVRHARGLEYVYPYAQPGTDREGCPRTW
                     FASRYRSANECPFKSSAALISRLQALTGELVDVAAWRMEFPIDENGYPRSDHRLVHPKS
                     AWWNCCFHLKLDVKQELGGGVHSHTDHDSWNPVGLDGWAGLVYLNPAPPTNQGGLRTWD
                     NRDPARQFEWMTSKHNWQLRDTFANVYNRLILHRGRVPHSGSNGWGDSFENGRFFQTFF
                     FRTLGQRELPSLDLEELNFG"
     gene            complement(5001..6872)
                     /locus_tag="O0S08_RS42750"
                     /old_locus_tag="O0S08_42750"
     CDS             complement(5001..6872)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="O0S08_RS42750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_42750"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_269035292.1"
                     /sec_met_domain="TIGR03975 (E-value: 8.2e-198, bitscore:
                     657.8, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 8.4e-17, bitscore: 61.2,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLFAVMPFGALRPAIGPSLLKAHLARRGIASKIVYLNIRFARS
                     IGLPDYEYVAERSPSQSLAGDWIFARSLSGERPDADARYLADFVRRFSKFGPVDRAIEI
                     LERCRDRAAGFLQKCLREVSWGDYDVVGFTSTFSQHAASLALARLVKAEHPRVKIALGG
                     ANCEESMGLQAHRSFPFIDFVCSAEADLSFPRLLEALAAGGDPSAVPGVIARRDGESRY
                     TTLTPDRVERMDDLPVPLYDDYFEQLPALGGVAKRGASVLMESSRGCWWGQKHHCTFCG
                     LNGTSMAFRSKSEQRVLREIDELEQLYGARTVVMVDNILDMKYFETLIPGLARRGLKLD
                     LFYETKANLSKDQLRAMQRAGITMIQPGIESLSSDVLRIMKKGISGVQNVQMLKWCREF
                     GITPHWNLIFGFPGEEPRDYEVMAEVLDLLHHLQPPYGVGQIRLDRFSPNFVRSSENGL
                     TNVRPDRSYGNIYALSESELFEFAYYFEHDYADGRDPETYVGPLRAAVKRWYANKGNKG
                     LLYVDHGDDLAVWDFRPGAHRTLTVLSGLERAVYVCCDQHRSLKQVVAACRADEREVRP
                     VLDGLVASRLVLHLDDRYLSLAVRKHGVGRDEDAEPRAPSSRSLAVV"
     gene            complement(6869..7648)
                     /locus_tag="O0S08_RS42755"
                     /old_locus_tag="O0S08_42755"
     CDS             complement(6869..7648)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012334.5"
                     /locus_tag="O0S08_RS42755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_42755"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_269041904.1"
                     /transl_table=11
                     /translation="MDASLARRRRTAVRRRATGVLEVTKRECLITGASGLLGSAWIARY
                     ASKYAITAVHRTREIEFATQDQSFFDPLAPRRPLDANDHAVHAVRADLATEKGCEQVVG
                     ETIARTTRLDLLLSAATVRMSAPLTSAPSLAAAEHAFALDVLAPLRLAAALVRASWGAD
                     PAANAAANRSVVLVAGTAGRYVYEDQGLGLHSATSAALCQLVYHLASELWHLGIRVNGV
                     LMPTNYCRDDAEKAATRIAGLDAGSATGTLVTLTEGP"
     gene            complement(7579..8352)
                     /locus_tag="O0S08_RS42760"
                     /old_locus_tag="O0S08_42760"
     CDS             complement(7579..8352)
                     /EC_number="1.14.11.-"
                     /GO_function="GO:0008198 - ferrous iron binding [Evidence
                     IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025043.5"
                     /locus_tag="O0S08_RS42760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_42760"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_269035293.1"
                     /transl_table=11
                     /translation="MDRLEDILARREWLRRSRPFPHVVARGVFKPRFYARLCEELGEVL
                     ARGLSETADGERFSRNIANYDSYGIGFRAGDGGALGVFLSPEWRDLMADCFGLQRTDYV
                     FAGAHHHAVGSGDGFVHNDYNPVWFPVSSEGRIQVPDHQRCDYKTGEGQLSPAEKIRVV
                     RGTAVIFFLLNGGWRPGDGGETGLYRSGRDPPGEPSVRVPPLDNSLLAFECSPDSYHAF
                     ISNRRATRTSVIMWTHRSLADAEQRFGVERLEYWK"
     gene            complement(8353..9078)
                     /locus_tag="O0S08_RS42765"
                     /old_locus_tag="O0S08_42765"
     CDS             complement(8353..9078)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF020604.5"
                     /locus_tag="O0S08_RS42765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_42765"
                     /product="Stf0 family sulfotransferase"
                     /protein_id="WP_269035294.1"
                     /transl_table=11
                     /translation="MESNRPARRYVIAGSQRSGSSLLCEGLEATGVAGHPAESFAPDFL
                     DHWFHRWGLVPDCPFADYLAAARRHGTGRNGVFGIKIQYMHVQSLARWAGRPRAGDAVL
                     ERLFPGARYVNIVRRDVRAQALSFYRAIHTNQWARTPGVAPAPPPALDPREVEQLERQL
                     TWQQTSWLQYFAARGIEPFTVYYESLAADYRGQIARVLEFLGLDGATARLLPPPVLIRQ
                     ADETTRRWREALDGRSDGR"
     gene            complement(9097..9972)
                     /locus_tag="O0S08_RS42770"
                     /old_locus_tag="O0S08_42770"
     CDS             complement(9097..9972)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012799.5"
                     /locus_tag="O0S08_RS42770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_42770"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="WP_269035295.1"
                     /sec_met_domain="TP_methylase (E-value: 1.1e-15, bitscore:
                     57.3, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTSTKHHAFDIAIVGLGVSGIHQMTREVEETIKRCRHVFVTDTA
                     EGVCDYLRTICPKVTELTVRGDLGAHRMVIYRRMASEVISGALDEGPVCFATYGHPTMY
                     CYPTELIQRAAQILDLRTMILPGVSSLDTLLSDLGIDPGGDGLQIYEASDMLIRQRPLQ
                     TDVGTVIYQVPIVLESSNRLPGKQSIDNLRRFQAYLLKYYPPKHTALFVLSKTHPLLET
                     VTQRIPLDRLADVMSTNTNLGTLYIPPVQHRAVVEHELAERMGAHDALDSPRRPGRPAI
                     GPKDPKARKA"
     misc_feature    10206..10219
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 21.45"
                     /tool="antismash"
     gene            10247..10519
                     /locus_tag="O0S08_RS42775"
                     /old_locus_tag="O0S08_42775"
     CDS             10247..10519
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS42775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_42775"
                     /product="hypothetical protein"
                     /protein_id="WP_269035296.1"
                     /transl_table=11
                     /translation="MAPAAVPAAVQAVVQAGAAVAAAADLEEAVAVAAVVAAVAAAAAG
                     LEEAAAVAAVAAVAVAAVAAVAAVVEAVATPREAKAGPDRSARHP"
     misc_feature    10650..10668
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.89"
                     /tool="antismash"
ORIGIN
        1 gggatcagcg actcgcagaa cgggtagacc gcggccggcg tcagcgtggt cgggtcgatg
       61 cggaagcgct tgttgtcggc gttgtcctcg gcgaacaggg ccttggcgat gaactcgcgc
      121 acctcggcgt acggcccctc gaccacgccg agcagctcgg acagcggcgg cgaccagcgg
      181 gccagctccc agcgcgcgag gtcgaggccg aactgccgga tcggcgcgcg gctgtcggtg
      241 agcagcggct tgacgcgggc ccacgacagg aactcggccg ggatctccgc gcccggatcg
      301 accggacgat cggtgagcgc gaggctgatg tcggggtggt gccggcggat gtactggcgg
      361 gcgaagtcgg cctgcggcgc gtcggccggg ccgggcccgg tggccatctc ccacagccgc
      421 tcgcagccgg cctgcaccga gtcgccgctg aagctgcgct gctcgccggc cagcatctgc
      481 aagaacgcgg tctcggagat gatcttgatc ggcgcgccct tctcgaccag cttctcggcc
      541 gcgacctgct tgctgccctt gcggccctgg ccgtacagcg gcgagccgtc gtcgccgatc
      601 accaggtagt cgagcttcgc cgtcacgctg ccggcgttct tgcccttggc gttcgtgacc
      661 ttctcctcgg cgtcgtcgcg cttcatcgtc gccagcttgc cggtgaagag gaaggtcttg
      721 ccgccgaggt cgatcgcctt gctcgcgccc ttggcctcgg ccttcggcgc actggcttcg
      781 ctggacttcg gcgcgaagtc ggcgggcacc agcgccttcg tcatgtattc gagggcgaag
      841 ttgcggtact gcggctcggg ccgcagcgcc agctgcatca gccagtcgaa gccgagctgg
      901 ctcggccgga accggcgcac gtcgctgagg aagccgagcg cggtggccgc gaggccctcg
      961 tcgaagttga gctcgcgggc ccacgggcgg ccgctggcga tgagctcggc gatccacttg
     1021 tcctcggccc acgtcggctg gaacgcgacc gcgcgccaga agccggcgtc gatgtcctcg
     1081 gccttgatct tctcctcgga gatccacttc agcagggtct ggcgcgcgta cgggtgcagg
     1141 accatgcgcg cgaggaagtc cttgccgagc tcctcgccgg ggaagcgcga cagcgcgtcg
     1201 agggcgaagt tggtcgcgtt ggcggagatc cgcgggtcgt cgagcaggtc gcggaagtac
     1261 tcggcgccga gcgacttgta ggtgtggatc ttcgccagca ggtccgaggc gaacgcgaac
     1321 accttctgcc ggctgtcgag caggcggtcg gtgaaccacg ccggcgtcag ctcgcgcgcg
     1381 ctgaagtgct tcctcagggc ggcgacggcg aggtcgtggc cgtgctgcgt gccgagcagg
     1441 cgaccccacg cgtcgaggcc gatgtccttg cgcgggtcac ggtcgccgag caggtcggcg
     1501 accagcttgc gcacctcggc gttgctgttg ttggccagca agatcaggcg gtcgagcgcc
     1561 aggtcgcggg cgtgggtgcg ggcgtagttg gccgcgtacg cgcgggccga gtcggcgcgc
     1621 gagtcgagca gctgcagcac cggctcgtgc aggccgagct cgcggaagct gccctggtcg
     1681 aacttcggca cgttcgacag cagccagatc acgaactcgt cgaccgtcgc cgagccgacc
     1741 gcgatcagcc gcgccgccca cggcgcctcg acctcgcgca ggttggcgcg gaagtcggtc
     1801 ttcagcgcct gcaccgcgaa cttgcggacc tgctccgagc gcgcccgctc gagcagctgc
     1861 agcaccggcc gcggggtccg ccgccacagc tcggcgaacg cccgctcgtt cagctgcggc
     1921 gcgcccgagc ggtacagctt gaagttgcgc cgcgtgtact tcttcgtctc gtgatggagg
     1981 atgtggttgt agacccacgt gttctgccac gtggtgctgt cctcgtagtg gcgcagcacc
     2041 gccgccgccg cgtcgacgta ggccggcgcc gcggcctcgc cgaggcgccg caggaaccgc
     2101 cacgcccgcc gcaccaggta gccgagcgtg tgccgcgaaa tctcgccgac cttgcgctgc
     2161 ttggtcgcgt acgcgtggtc gaagcggacc gcgagcgcgc cgaggatctc gacgtcgctc
     2221 ttggcctcgg cctccttgaa gatctgcttg agcccgcgcc acggacccca gcgcagcggc
     2281 accttctgga tcacgtcgag cagcgcggcc cgctcgtagg cgccttcgga gcgcttccac
     2341 agatccagca gcacctcgtg cagctgcagc cgcgggggca gcggagtctc ggcgtccttg
     2401 gcctcgagcg cccgcatgct gtcgatgcgg tagcgagccc gctcctgcgg cttggagcgc
     2461 acgaacttcc agctcgtcag gtgctgggtg aagctcgcaa aggtcagcgc gccttctcgt
     2521 ggcggcttgt ccggccgcgg gtcggccgcg ttggcgaggc tcacgatcaa ggtggacagg
     2581 tccgggtccc ggctgtccca cgcgcggcgg atatcatcga ggctgagttc gagggccatc
     2641 gcctgctccc gagagaggga gccggagcat gccgcgaagg gccgagcgct ttcaactgtc
     2701 agcgtcgcgc ctcggctgtc catttggaca ggtcaaacgc tcgacttcgc gccccgcgat
     2761 cgggacatcc ccttaaagtc gcctggctcg aggggcacat ccttgcaggt acagatcgcg
     2821 ccgagatcct cccctcgggc cgtgataaaa gcgtcggcat gacgaccacc cggacctcgt
     2881 ggaccctcgc gctctctctc gccaccctcg tcgccctgcc gagctgcaag aaggaagcga
     2941 cgacgccccc cgacgggtcc gcaggcggcg acgcggcggc cgggagcaac gacggcggcg
     3001 gcggcaacgg cggcaacgag ggcgcgagcg aggagcccct gaccgccgac tcgttcgagg
     3061 aggcgatgca ggccaggcag ggcgacgtcg ccgactgctt cgcccaggcg aaggaggcca
     3121 agcccgacct cgccggcaag ctcgccctcg acgtcaccgt cgccggcgac ggctcggtct
     3181 ccagcatcaa gttcaccgac acgagcacga tcaaggaggc cagcatcacc tcctgcatcg
     3241 aagagaaggc caagggctgg aagttcaaca agcccagcga cggcaacccg atgaccctcg
     3301 gctactcgct caacctgtcg tagaccgggc ggtgagaccg ggcgcgcgat cgcgcgtgaa
     3361 tttttaaaca ttacccaaaa gacatcctcc tcgacatcga ccgccgcggc ggtcacgact
     3421 ccggcgtgac cgttgcgcgc gcaagctcgc cgaggaactc ggcgaagcgg tcgcgtgggg
     3481 agtacccgag ctcgctgcgg atgcgcgtgc ccgcgacgcg catggcgggc gcgtcgtcgg
     3541 gcgggtcggc gggtcgccgg ccggtcgcag ccgcgagcgc ttcgtggtag gcgcgggtct
     3601 cgccgtgtcc gtcgaccacg tcgaagacgc ctcccacggc gcgcgggtgc gtggctgcga
     3661 gcagcgctgc gtccgccagg ttgtcgacgt gcacgaacgg catcgtccgg ctcgcgaatt
     3721 gcggctgcgc gctccgacgc gcgcgctcca gcgccagcgg cccccagaac gacgagcggt
     3781 gcatcgacag gacaggcacc ggccggagga cgacccaggc gagcccgtgg tcgtccgagc
     3841 ggacgagctc ctccgcctcg accttcgagc ggccgtacgg atacacgtcg tcggaccact
     3901 gcgggctctg ttcgtccacc gaccagccgt ggcggacgtc gtacaccgag acgctcgaga
     3961 cgaacacgaa gcgcgcgcga cgggacgcgc ccagcgccgc caacaagtgg cgagtgccct
     4021 cgacgttggt ctcacggcac atcctgcggt cggacgacag ggcggccgcg cagtggacga
     4081 ccacgtcggc gccccgacac gcgacgcgca acgcgtcggc gtcacccagg tcggcctgca
     4141 ccaactccac gggcgcgccc ggtagcgccg gcgacctgac caggccccgc acgccccagc
     4201 ccgccgcggc cagtctgtcg cagacccggc tcccgacggt gccggcggca cccgtgacga
     4261 cggcacgcgg tctcatccga aattcagctc ctcgaggtcg aggctcggca gctcacgctg
     4321 cccgagcgtg cggaagaaga acgtctggaa gaagcggcca ttctcgaacg agtcgcccca
     4381 gccgttcgag ccgctgtgag ggacccggcc gcggtgcagg atcagtcggt tatagacatt
     4441 ggcgaacgtg tcgcgcagct gccaattgtg cttcgacgtc atccactcga actgccgcgc
     4501 agggtcgcga ttgtcccacg tgcggagccc gccctggttg gtcggaggcg ccgggttcaa
     4561 gtagacgagc cccgcccatc cgtcgaggcc caccgggttc cagctgtcgt ggtcggtgtg
     4621 gctgtggacc ccccctccga gctcctgctt cacgtcgagc ttcaggtgaa agcagcagtt
     4681 ccaccacgcg ctcttcggat ggacgaggcg atggtcggac cgggggtagc cgttctcgtc
     4741 gatcgggaac tccatccgcc acgcggcgac atcgacgagc tcgccggtca gcgcctgcag
     4801 ccgcgagatc agcgccgcag acgacttgaa cgggcattcg ttcgcgctgc ggtacctcga
     4861 ggcgaaccat gtccgtggac agccctcgcg gtcggtgccg ggctgtgcat atgggtagac
     4921 gtactccagg ccgcgggcgt gacggacgac cgcggccggg tcggcgtaga agtcgtcgac
     4981 gacgatgacg tcccgccgca tcacaccacc gccagactcc gcgaagacgg cgcgcgcggc
     5041 tccgcatctt catcgcgccc gacgccgtgc ttgcgcaccg cgaggctgag atagcggtca
     5101 tcgaggtgga gcacgaggcg agacgcgacc agcccgtcga gcacgggccg gacctcgcgc
     5161 tcgtcggcgc ggcacgccgc gacgacctgc ttcagggagc ggtgctggtc gcaacagacg
     5221 tacaccgcgc gctcgagccc gctcaggacg gtcagggtgc ggtgcgcgcc cggtcggaag
     5281 tcccacaccg ccaggtcgtc gccgtgatcg acataaagca ggcccttgtt gcccttgttc
     5341 gcgtaccagc gcttgaccgc cgcgcgcagc ggcccgacgt acgtctcggg atctcggccg
     5401 tcggcgtagt cgtgttcgaa gtagtacgcg aactcgaaca gctccgactc ggacagcgca
     5461 tagatattgc cgtagctgcg gtcggggcgg acgttggtga gcccgttctc gctggagcgc
     5521 acgaaattgg ggctgaaccg atcgaggcgg atctgcccca ccccgtacgg aggttgcagg
     5581 tggtggagga ggtccaggac ctcggccatg acctcgtagt cgcgaggctc ctcgccgggg
     5641 aagccgaaga tcaaattcca gtgcggggtg atgccgaact cccggcacca cttcagcatc
     5701 tgtacgttct gaacgccgct gatccccttc ttcatgatcc gcagcacgtc cgagctcagg
     5761 ctctcgatgc ccggctggat catcgtgatg ccggcgcgct gcatcgcgcg gagctgatcc
     5821 ttcgagaggt tggccttggt ctcgtagaag aggtcgagct tcaacccgcg gcgcgcgagc
     5881 cccggaatga gggtctcgaa gtacttcatg tccaggatgt tgtcgaccat gaccacggtg
     5941 cgcgcgccgt acagctgctc gagctcgtcg atctcgcgca agacccgctg ctcgctcttg
     6001 ctgcggaaag ccatggacgt gccgttcagc ccgcagaacg tgcagtgatg cttctgcccc
     6061 caccagcacc cgcgcgagga ctccatgagc acgctcgcgc ctcgcttggc gacccctccc
     6121 agggcgggca gttgttcgaa gtagtcgtcg tagagcggga ccgggaggtc gtccatgcgc
     6181 tcgactcgat ccggcgtgag ggtggtgtag cggctctccc cgtcgcgccg cgcgatcacg
     6241 ccggggacgg cggacggatc gccgccggcg gcgagcgcct cgagcaggcg gggaaagctg
     6301 aggtccgcct ccgccgagca cacgaagtcg atgaagggga acgagcggtg cgcctgcagc
     6361 cccatcgact cctcgcagtt cgccccgccg agcgcgatct tcacgcgcgg gtgctcggcc
     6421 ttgacgagcc gcgcgagcgc cagcgacgcg gcgtgctgcg agaacgtcga cgtgaagccg
     6481 accacgtcgt agtcgcccca gctcacctcg cgcaggcact tctgcaggaa cccggccgcg
     6541 cggtcgcgac atcgctcgag gatctcgatc gctctgtcca ccgggccgaa cttcgagaag
     6601 cggcggacga agtccgcgag atagcgggcg tcggcgtccg gacgctcgcc ggacagcgac
     6661 cgcgcgaaga tccagtcgcc cgcgaggctc tgcgacggcg accgctccgc gacgtattca
     6721 tagtccggga ggccgatgct ccgcgcgaat cggatgttca aatagacaat cttgctggcg
     6781 atgccgcgcc gcgccaggtg ggccttgagc aggctcggcc cgatcgccgg acggagggcg
     6841 ccgaacggca tcacggcgaa caggaccttc acggcccctc cgtgagcgtg accagcgtgc
     6901 cggtcgccga cccggcgtcg agcccggcga tcctcgtggc ggccttctcc gcgtcgtcac
     6961 ggcagtaatt cgtcggcatg agcacgccgt tgacgcggat accgaggtgc cacaattcgc
     7021 tggcgaggtg atacaccagc tggcagaggg ccgcgctcgt ggccgaatgg agccccagcc
     7081 cctggtcctc gtagacgtat cggccggcgg tgccggcgac caggacgacg ctccggttgg
     7141 cggcggcgtt ggccgcagga tcggcgcccc acgacgcgcg gacgagcgcg gcggcgagtc
     7201 gcaatggcgc gaggacgtcg agcgcgaagg cgtgctcggc ggcggcgagc gacggcgccg
     7261 aggtgagcgg ggccgacatg cgcacggtcg cggccgacag gaggaggtcg agccgggtgg
     7321 tccgggcgat cgtctccccg acgacctgct cgcacccctt ctcggtcgcc aggtcggccc
     7381 ggaccgcgtg gacggcgtgg tcgttggcat cgagcggccg ccggggcgcg agcggatcga
     7441 agaacgactg gtcttgagtc gcgaactcga tctcgcgtgt gcggtgcacc gcagtgatcg
     7501 cgtatttcga cgcgtatcgc gcgatccatg cgctccccaa caggccgctc gcgccggtga
     7561 tcagacattc acgctttgtc acttccagta ctccagtcgc tcgacgccga accgctgttc
     7621 ggcgtctgcg agcgagcgat gcgtccacat gatgacggat gtgcgcgttg cgcgacgatt
     7681 gctgatgaac gcgtggtatg aatcgggcga acactcgaag gcgaggagac tgttgtcgag
     7741 cggcggcacg cgcacgctcg gctcgcccgg cgggtcgcgc cccgagcgat aaaggcccgt
     7801 ctcgccgccg tcgccggggc gccagccgcc gttcaacagg aaaaagatca ccgccgtccc
     7861 gcggacgact cgaatcttct ccgcggggga cagctgccct tcgcccgtct tgtagtcgca
     7921 cctctgatga tccggcacct ggatccgccc ctccgacgag acggggaacc agaccgggtt
     7981 gtaatcgttg tggacgaaac cgtcgccgct gccgacggcg tggtgatgcg cgcccgcgaa
     8041 cacgtagtcg gtgcgctgga ggccgaagca atctgccatg aggtcgcgcc actcgggtga
     8101 aagaaagacc ccgagcgcgc cgccgtcgcc cgcccgaaag ccgatcccgt aggagtcgta
     8161 gttggcgatg ttccgcgaga atctctcgcc gtccgccgtc tcgctgaggc cgcgcgcgag
     8221 gacctctccg agctcctcgc agagccgggc gtagaaccgg ggcttgaaca cgccccgggc
     8281 gaccacgtga gggaagggcc gcgagcgacg cagccattca cggcgggcga ggatgtcttc
     8341 cagtcgatcc attcatctcc cgtcgctacg accatccagc gcctctcgcc atcgtcgagt
     8401 cgtctcgtcc gcctggcgga tgagcaccgg cggcggcaag agcctcgccg tcgcgccgtc
     8461 cagcccgagg aactcgagca cgcgcgcgat ctggccgcgg tagtccgccg ccagcgactc
     8521 gtagtacacc gtaaaaggct cgatcccccg cgccgcgaag tattgcagcc acgaggtctg
     8581 ttgccacgtc agttgccgct ccagctgctc gacctcgcgc ggatcgagcg ccggaggcgg
     8641 cgccggggcg acccccgggg tccgggccca ttgattcgtg tgaatcgccc ggtagaacga
     8701 gagggcctgg gcccggacgt cccggcgcac gatgttgaca tagcgcgcgc cggggaacag
     8761 gcgctcgagc acagcgtcgc cggcgcgcgg cctgcccgcc caacgcgcga gcgactggac
     8821 gtgcatgtac tggatcttga tcccgaagac cccgtttcga ccggtgccgt gccggcgtgc
     8881 ggcggcgagg tagtccgcga acgggcagtc cggcaccagg ccccagcgat ggaaccaatg
     8941 gtcgaggaag tccggcgcga acgattcggc cgggtgccct gcgaccccgg tggcctccag
     9001 accttcgcac aggaggctgc tcccgctccg ctggctgccg gcgatgacgt aacgccgcgc
     9061 tggtcgattg ctctccatgg gcctccgttc gtacgactac gccttccgag ctttcgggtc
     9121 cttcggaccg atcgcggggc gacccgggcg gcgaggtgaa tcgagcgcgt cgtgagcgcc
     9181 catccgctcg gcgagctcgt gctccacgac cgcgcggtgc tgcacgggcg ggatgtagag
     9241 ggtcccgagg ttggtgttgg tcgacatcac gtcggccagc cggtcgagcg ggatccgctg
     9301 cgtgacggtc tcgagcaacg ggtgcgtctt cgacagcacg aagagcgcgg tgtgcttggg
     9361 cgggtagtac ttcaacaggt acgcctggaa gcggcggagg ttgtcgatgc tctgcttgcc
     9421 cgggaggcgg ttgctcgact ccagcacgat cgggacctgg tagatgaccg tccccacgtc
     9481 cgtctgcagc ggccgctggc gaatgagcat gtcgctcgcc tcgtagatct gcaggccgtc
     9541 tcccccgggg tcgattccca ggtcggacag cagcgtgtcg agcgacgaga cgcccggcag
     9601 gatcatcgtc ctcaggtcga ggatctgcgc ggcgcgctgg atgagctccg tcgggtagca
     9661 gtacatcgtg gggtgcccgt acgtggcgaa acagaccggg ccttcgtcga gcgcccctga
     9721 gatcacctcc gacgccatgc ggcgatagat gaccatcctg tgggccccga gatcgccccg
     9781 tacggtgagc tcggtgacct tcgggcagat ggtacgaagg tagtcgcaga cgccctcggc
     9841 ggtgtcggtg acgaacacgt gccggcagcg cttgatggtc tcctcgacct cgcgggtcat
     9901 ctggtggatg cccgagacac ccagacctac gatggcgatg tcgaatgcgt ggtgcttggt
     9961 cgacgtgttc atccgagctt ctccatcaaa cgggcgacgg ccacgcgcct ctcggccgag
    10021 cttcgagctc cgcgaatctc tttcatggct ttcacaactt ccggatcggc gatgagatcg
    10081 tcgatgacaa agtccttcca gcgctgaaag ctgttgaacc agtcgtggtg gccaatgacg
    10141 ttgaccccga aaccgggccc cccgggcggc ggtcccggcg gcggtcccgg cggcggtccc
    10201 ggaactggac cgactccaaa cgacaccccg ggcggcggcc ccggcggtgg ccccggcggc
    10261 ggtcccggcg gcggtccagg cggtggtcca ggcgggggcg gcggtggcgg cggcggcgga
    10321 cctggaggag gcggtggcgg tggcggcggt ggtggcggcg gtggcggcgg cggcggcggg
    10381 cctggaggag gcggcggcgg tggcggcggt ggcggcggtg gcggtggcgg cggtggcggc
    10441 ggtggcggcg gtggtggagg cggtggcaac tccgagggag gcgaaggccg gtccggatcg
    10501 gagcgcacgc cacccatgat cagccgccag agttcggctc gatcgacgat cagcgctcgt
    10561 acctcgtggt ccttgagctc gtagggcgcg agcgccttac gagggtttga ttgcagcgca
    10621 taaaagaaat cgaagtccgt ttgcgcgcga tcgagaacgc gtttgaaatc actcatgtcc
    10681 cctccactgg gaacgcatcc cctataggtg gccccagtgt cgctccacgt caaacccgcc
    10741 taacagcatt catgcgtcgc aacaaatcgc gcgtgcaggc gcctttccgg gtccagggag
    10801 cagtcacgag ccgtgttcgg acgatgtcgc gaatgtgaaa tggagcgctc cgattacggg
    10861 gtcctcgacg acagactccg cgagcgcgcc ccggcgccgg ccgagcagat ctccgggcat
    10921 atgcgtcgag gtgccgatcc ggcgtgtggg catattcgca acgcggcccc ggccccccaa
    10981 tcgtcagcca ggcctacccg tgatcggcgc atccgcatgg tatgaccatc acggcgtacc
    11041 tcggattgcg acgagtcctc gcgccggcgg tttcacgtcg cggccatatc cgcgccgagc
    11101 atgaggacag cctcgtcctc acgatgcgca gcgaatccgc gcactgtatt cgctcgggcg
    11161 gcatggtacc cgagagagtc atcgattcgc ggagctccga ggggacgatg cagccgcagc
    11221 cgtcgaggcc cgcagcgcat cgactgcgac tgcccgagcc gtcgcggcga tcgtgagccg
    11281 ggccggcgat gcgtcgatgc aaccgaggga ggcgcgggcg cgacgcccga gcaggcgtcg
    11341 ttcgctgctg cgttgatgac gtgcacgcag cagaactgct tgtgagcggg acgccgcaga
    11401 ctcgggcgag ttcgcggtgt ggccgtacgc gaccgcccga gcctggcgag atggtccgtc
    11461 gcggcgagcg ggcgcttgcg tgcggacgag ccgcgcgagg cggcgcgatc gaggtagaag
    11521 ggtgtgtgcg atcgaatcct ctcgcgggct tcttcaccgc cgacgctgcg gcgatcctcg
    11581 gcgcgcagga ccggctggcc gcggccgatg aaccggcgcg cgcgcgggtc gtgaggccga
    11641 gtccggtgcc gttcctcccg ggcatgcgag cggcacccga gggcctgtgg tcggagccgt
    11701 acttcggccc ggtggaggcg cgcgatccgg cggcctgggg cgtgatcgaa ttgccgcgac
    11761 cggtggttca cccggggctg gtgccggcga tcgccggggt gctcgggctg cgcgcgagcg
    11821 aggtttgggc ggtcgccgag gtacgggcgt ggatcgacgc cgagggccgg gt
//
