LOCUS       NZ_CP114040            11884 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Nannocystis poenicansa strain FL3 chromosome, complete genome.
ACCESSION   NZ_CP114040
VERSION     NZ_CP114040.1
KEYWORDS    RefSeq.
SOURCE      Nannocystis poenicansa
  ORGANISM  Nannocystis poenicansa
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Minimal conservation of predation-associated metabolite biosynthetic
            gene clusters underscores biosynthetic potential of Myxococcota
            including descriptions for ten novel species: Archangium lansinium
            sp. nov., Myxococcus landrumus sp. nov., Nannocystis bai
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2022) Biomolecular Sciences, University of
            Mississippi, Faser Hall 407, Oxford, MS 38655, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: IPA v. 10.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: PacBio Sequel IIe
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026965555.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 12:45:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,229
            CDSs (total)                      :: 10,129
            Genes (coding)                    :: 10,068
            CDSs (with protein)               :: 10,068
            Genes (RNA)                       :: 100
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 9 of 61
            Pseudo Genes (incomplete)         :: 52 of 61
            Pseudo Genes (internal stop)      :: 2 of 61
            Pseudo Genes (multiple problems)  :: 2 of 61
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:28:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5888356
            Orig. end                         :: 5900240
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11884
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="18"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11884
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11884
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6884](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6884
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(452..469)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.15"
                     /tool="antismash"
     gene            complement(770..1366)
                     /locus_tag="O0S08_RS22695"
                     /old_locus_tag="O0S08_22695"
     CDS             complement(770..1366)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /locus_tag="O0S08_RS22695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_22695"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_269041310.1"
                     /transl_table=11
                     /translation="MKDLTDTRHTAKRDGPQVLDAARLFRDHAEFVANFAVRLGVGRPE
                     VDDVVQEVFLVAHRRGGFVPGPARPTTWLAEITLRVVSASRRKLRRSWEDADTEAVAAA
                     SAQAGSSPGEQAEASEALRRVQQALDTLEEGKRAVFILFELEGESCEAIAAGLGIPIGT
                     VYSRLHKARKQFMEAHARLVAGPPRPLTRSQTVTS"
     gene            complement(1363..2145)
                     /locus_tag="O0S08_RS22700"
                     /old_locus_tag="O0S08_22700"
     CDS             complement(1363..2145)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS22700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_22700"
                     /product="hypothetical protein"
                     /protein_id="WP_269041311.1"
                     /transl_table=11
                     /translation="MAIALEIRGELVERTLDPPRPTCSDLHALVGLAIAIAVDAAVLEG
                     LGYEVIEPGEGSVPQAQDPERPPLQRRSRRGETSEVPVRRASVSVVAAVRGGAWLGVLP
                     GLAGGGSVQLELGWRRWVDLRAGLFGGYGGPRALDADTVVTPSLVGGRIDVCAALARPR
                     VRPRLCFGPAAGALQSGAKSPGVQAVVAPWVALMVAPELRIWAAKRFAFDLAVELVVPV
                     VRPVLAERDPSKQGMVGESLPVPPVGAVVSLGVAFTIR"
     misc_feature    complement(2096..2111)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.69"
                     /tool="antismash"
     misc_feature    2097..2110
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.36"
                     /tool="antismash"
     gene            2429..3100
                     /gene="elbB"
                     /locus_tag="O0S08_RS22705"
                     /old_locus_tag="O0S08_22705"
     CDS             2429..3100
                     /EC_number="4.2.1.-"
                     /codon_start=1
                     /gene="elbB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125971.1"
                     /locus_tag="O0S08_RS22705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_22705"
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="WP_269041312.1"
                     /transl_table=11
                     /translation="MQHKRVGVVLAGCGYLDGAEITEAVLTLYFLERAGLEPRCFAPDR
                     PQMHVVDHLTGQPTGETRNVLVESARIARGKVEPLDRARMEELDALVLPGGYGVAKNLS
                     DFAVRGPEADVEPELVRLVGEALSRAKPLVAICISPAILAAALRKLGIGGARLTIGDDD
                     GTAAAVTALGARHEACTVDRAIVDERHRIISTPAYMLGPGPRDVGAGIEQAIATLAGWL
                     R"
     gene            3155..4066
                     /locus_tag="O0S08_RS22710"
                     /old_locus_tag="O0S08_22710"
     CDS             3155..4066
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739070.1"
                     /locus_tag="O0S08_RS22710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_22710"
                     /product="hypothetical protein"
                     /protein_id="WP_269041313.1"
                     /transl_table=11
                     /translation="MSRRFPRALVFLLALASACGGEPVKLAAKAEKLEVKPPPPAPTTV
                     HLAIEGSSGKLGFDMEAPIEKIRGKVPASALSGEVWLDPTDLAKSRGLVHVDLRDLELF
                     QRTAPEEGKEFGEEVKNDTQNEHARQWLEISPDTPADELAKNSVVEFALTRISDASATD
                     LSKVTGPERKVTFTASGDFLLHQRKAEKSVKLEATFRYDGDALREVHVRSVEPLVIGLD
                     EYDVRPRTGFSKLAAKTLEQLAPKVAKEAAVSLEFTARPDGKPAIPEAAAPANSGPPAA
                     PSPAPATPSAPAAEPAPAAAAK"
     gene            4128..4568
                     /locus_tag="O0S08_RS22715"
                     /old_locus_tag="O0S08_22715"
     CDS             4128..4568
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS22715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_22715"
                     /product="hypothetical protein"
                     /protein_id="WP_269041314.1"
                     /transl_table=11
                     /translation="MDIRTISATLALLGSGLTLGGCNKTQTDATEVPGNAGAAGEKSCK
                     GHADSEKSCGGHAEGEKSCGGEKGCGGEKGCSGVKGDTTCAASKPTEAAETAPAADPAT
                     TATATAPAATAAEPTATKSEKKKKPAAKKAKGEAACGEGTCG"
     gene            5001..6884
                     /locus_tag="O0S08_RS22720"
                     /old_locus_tag="O0S08_22720"
     CDS             5001..6884
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="O0S08_RS22720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_22720"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_269041315.1"
                     /sec_met_domain="DUF692 (E-value: 4.9e-84, bitscore: 280.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRPTGIGLGLRWDFLDEALAAPVDPALAFFEISPENYMRRGGYF
                     PAALETIAERRPLLTHGLMLNVGAATGLDDAYLAELRSFLDALRVAEHTDHLCWTGRGG
                     VCLHDLLPLPFTRAAARQVADQIRRAQDALARPLALENISYYAAAGASRGSDRGAALAA
                     RELEFLHEVLSRADAGLLLDVNNVVVNATNHGFDPDAFVADLPLERVVRLHVAGGEVRP
                     HLGGLLIDTHGAPVPPAVGALMGRVVARLGPLPVLYERDHNIPDYAKLVAEVRDLAGIY
                     DAALAGESRPKAHVPEDIRLTGTSEGSSHGSNSGDEAEARPDAVLAARPPAEPRDSSLA
                     GPASAAPHDDLSADERAELDDIQRGVSHLIVDLEAESPARRGLSEFLRARGVGPAAVAA
                     LAEVDPERLLVYRQLIRRQVRGVLADLLSGTAAARGQVAFDADADAWIAELGPRSRLLR
                     DLPGEFVAWATPRWSNEHVLEDLPEAPAWLLDLARHELLTHEVGASPPRPPGDERPFGL
                     DARLEFDPSVRLGRFAFAVHEHPDAPDVAPRREPTALLLYRDREHAVRTLHLSPLAEAL
                     LDALLLRGVTVEQAVREAAEATGSAIDGDFLGRVSVLLADLSERGAIRGAR"
     gene            complement(7094..8008)
                     /locus_tag="O0S08_RS22725"
                     /old_locus_tag="O0S08_22725"
     CDS             complement(7094..8008)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS22725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_22725"
                     /product="hypothetical protein"
                     /protein_id="WP_269041316.1"
                     /transl_table=11
                     /translation="MTLAHIRAVLICLTGVLAATPALAQEPAPAASAPAPATAAPAPVP
                     APAGETVATPAPTPESGVSVPPPSAAPPPVSPPVAAPPTTAPTAGAPPGPAPAAGAAPS
                     FDWGGRQVEPLPPPPAPADPSKIRRDSWRGRFWIAPRLTITGPIGGDKPARPTLLTLGG
                     GFDFGVRINNRLGVGTGLSGQTHTSIRTTLPGTTDKTIKNGGMFFWDVAFVRLYFMKKR
                     FQPLMELGVGYARARLPLGDVLHGAQIRAGLGFDAWVSNQVTLGFTTVYRMIALNMPKD
                     GITPARWEVGHALQGVLQLGLHW"
     gene            complement(8005..8796)
                     /locus_tag="O0S08_RS22730"
                     /old_locus_tag="O0S08_22730"
     CDS             complement(8005..8796)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS22730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_22730"
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="WP_269041317.1"
                     /transl_table=11
                     /translation="MRLSLALPGEAADLEGPVSEALQVALRDSAIFAPDGAPGSTVQGE
                     AALTREAADAPWVLHVGMAVPDDLRSKFAVAALTTSATAGTDAFRPSTEALTDAARRAI
                     AGLEAQCRLARGDASGLDVLLSSGEPSQILVALRYVGDREAREHAHRLLPLLRSDDMRV
                     RMAVLDVLGSVGTRDHAAAIVREVHRLDPGATREAYRALARLGGADAVGFLRFAAANED
                     DPELRAEAERALSSALSGKPPSAAEVPRGVDLPKLARGHRQ"
     misc_feature    complement(8026..8028)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(8871..9554)
                     /gene="lipB"
                     /locus_tag="O0S08_RS22735"
                     /old_locus_tag="O0S08_22735"
     CDS             complement(8871..9554)
                     /EC_number="2.3.1.181"
                     /GO_function="GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="lipB"
                     /inference="COORDINATES: protein motif:HMM:NF046470.1"
                     /locus_tag="O0S08_RS22735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_22735"
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="WP_269041318.1"
                     /transl_table=11
                     /translation="MASTPESLRAVWLGRMAFEEALALQLAARERVVAGEPGVLFLVEH
                     PPVLTLGRRARREDILWSEEQRAAAGVTIWEAPRGGEVTLHGPGQLVAYPIMMIGRRIR
                     EHIVRLGDVAIAVLGELGVDGCEFRMEHPGVWRGTEKLASIGIHVSRGVTVQGISVNLA
                     VDPALFGALVSCGLHGVTVTSAVAVGGRETTVEAAGRRFAALYAAQLGLPLVWEGAEGY
                     GSDRR"
     gene            complement(9538..11256)
                     /locus_tag="O0S08_RS22740"
                     /old_locus_tag="O0S08_22740"
     CDS             complement(9538..11256)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016924.5"
                     /locus_tag="O0S08_RS22740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_22740"
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_269041320.1"
                     /transl_table=11
                     /translation="MTFLEAAIEVLRHESEPLHFSEIVKRAVDRQLLSHVGRDPVAAMQ
                     ASLNAAVRGDSALLVRSKPGFFQLRPGAAPPPAPAPPVPEPPPPPAAPPTPPPTLELPF
                     ETAPVSAKKSNDVEALPPKVEANSMLTEDTRDEEETSEADEGQAEEGGGRRRRRRRDRF
                     GVRGEAPAPQQIAIKPTRRGLDTGKRAEVIQRVTPNLEFEAPKGAGLDGVTDVAVVMAN
                     AMSRLAEERPELRTELESMQRPPPPPPPSAPAVEHRSLRRPIGAPPPPPRPQPQPQVQP
                     QAASEDEDRPGRRRRRRRRRGRRNEGGVAEIRTMRDEASESLLQQVAQVMTDAGPRSLH
                     IRQIAETLAGMGVLGGEISEIERAVTAAILLDIRHGGRASRFIARGDARYQLHASRVPP
                     AVTAAEQALHAAITALNNEIANAFVQWLQTLGPRGLEAVVRIWLTREGYPVLATLPPSR
                     GIAKLVIGDIEGDPDSDSEEDEANPKLLALILPRRAGIDASAWAGDLERTQASGCLVFY
                     MGDAPAEAPWGDARVVTASELAGWLRTQGVGISPLPVAIGALDPTVLESISGLDT"
     misc_feature    11294..11307
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.18"
                     /tool="antismash"
     misc_feature    complement(11295..11308)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.99"
                     /tool="antismash"
     misc_feature    11295..11309
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 20.58"
                     /tool="antismash"
     misc_feature    complement(11406..11420)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.85"
                     /tool="antismash"
     misc_feature    complement(11407..11422)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.12"
                     /tool="antismash"
ORIGIN
        1 cgcacctggg ccgcgtaggg cccgttcgga aaacggctca ggaacgccgc tgcgcgccgt
       61 tctgcctcac ttcgacggtt cagctcgaag agggccagca ccgcgacgcc ctcccattcc
      121 tgggcgaact gcccggcctt gaactcggtc gcccccgcct cggcccggga gagcgcctcg
      181 gccggatcgc gcccgaggaa cccgcgggca gcctgcaggt cgcgggcctc gcggaggtag
      241 tcggccgacc cgccaccggc gcgggccgcc gctcgcgcgt cggtccgggt cgccttggcc
      301 gcgcgcacct tcttgccgtc gccgcgggcc gctggctttt cggacatgtc ctcggcgacc
      361 tcctccggcg cgctcgccgg agctgcctct tgggccatgt cctccacgac agccaccggc
      421 gcgagcacgg ccggtgcgcg tgctggctcg gaggacatgt ccttaacaac agcttccggc
      481 gctcgcccct cgcccgggcc gccgagcgcc ggcggcggcg tggtcgtcgt cggctgcgac
      541 agccacagcc ccccgccgat gagcccgccg aggatcagcg ccccgaggcc gagcgtcccg
      601 gcgccccagc tcgaggaggc cgcgccggcg cccttggcca gcgaggcctc gaagcgggcc
      661 gcgccggcgc cggcgtcgta cggcacgcgg tggcgagcag cggccgcgag gtcgcggcga
      721 agcccggcgg aggcggtcac gtcgtcgagg aggcgggtgg gctcttggtt catgacgtca
      781 ccgtttgcga gcgagtgagc ggccgaggcg gccccgcgac caggcgggcg tgggcctcca
      841 tgaattgctt gcgggccttg tgcagccgcg aatacaccgt tccgatgggg atccccaggc
      901 cggcggcgat cgcctcgcag ctctcgccct cgagctcgaa gaggatgaac acggcgcgct
      961 tgccttcctc cagggtgtcg agcgcctgct gcacgcggcg cagcgcctcg ctggcctcgg
     1021 cctgctcgcc gggagagctg ccggcctgcg cggacgcggc ggcgacggcc tcggtgtcgg
     1081 cgtcttccca cgatcgccgc agcttgcggc ggctcgcaga caccacgcgc agcgtgatct
     1141 cggccagcca ggtcgtcggt cgcgcgggcc ccggcacgaa gccgccgcgg cggtgggcga
     1201 ccaggaagac ttcctggacc acgtcgtcga cctcggggcg accgaccccg aggcggaccg
     1261 cgaagttggc gacgaactcc gcatggtcgc ggaacaggcg cgccgcatcg aggacctgag
     1321 ggccgtcacg cttggctgta tgtcgcgtgt ccgtcaaatc cttcaccgga tcgtgaacgc
     1381 tacgcccagg ctcaccacgg cacccacggg cggcaccggc agcgattcac cgaccatacc
     1441 ttgtttgctc ggatcgcgct cggcgagcac cggccgcacc accgggacga ccagctcgac
     1501 cgcgaggtcg aaggcgaagc gcttggcggc ccagatccgc agctccggcg ccaccatcag
     1561 cgccacccac ggggcgacca cggcttgcac gccgggcgac ttcgcgccgc tctggagcgc
     1621 accggcggct ggcccgaagc acaggcgagg ccgcacacgc ggacgcgcaa gcgcggcaca
     1681 cacgtcgatc cgaccgccca ccaggctcgg cgtcaccacc gtgtcagcgt cgagcgcgcg
     1741 aggccccccg tagccgccga acagccctgc acgcaggtcc acccacctgc gccacccgag
     1801 ctcgagctgc acgctgccgc cgcccgcgag tcccggcaac accccgagcc acgccccgcc
     1861 gcgcaccgct gccaccaccg acaccgacgc gcgacgcacc ggcacctcgc tcgtctcgcc
     1921 tcgccgcgac cgccgctgca gcggcggccg ctccgggtct tgcgcttgcg gcacgctccc
     1981 ctcgcccggc tcgatcactt cgtagccgag cccctcgagc accgccgcgt cgaccgcgat
     2041 agcgatcgca agcccgacga gcgcatgcag gtcgctgcac gtcggtcgcg gcggatcgag
     2101 cgtgcgttcg acgagttcgc cgcggatctc gagcgcgatg gccaccgagt gcggccgcgt
     2161 cggatcgccg cggacgtcga tcctcgcgac cgacgcatcg acggtgtcgc gacccaacca
     2221 ggtcgtcgct gcgtccgcga gcgcgtccgg cgtcaggcac gtcgcgccgg gttcgacgcg
     2281 cagcgcctcc cgcagcgggc gcgggggcgg aggcgacggc gcgggcgcga gcagcaggcc
     2341 ggcggtgaac agggcgacga gcaccgcgcc caggatgcgt cgcctccccc gccgttcgcc
     2401 agtcgcatct cgtggtaagg tccccgcgat gcagcacaaa cgcgtcggag tcgtcctggc
     2461 cggctgcggc tacctcgacg gagccgagat caccgaggcc gtcctcaccc tctacttcct
     2521 cgagcgcgcc ggtctggagc ctcgctgctt cgcccccgat cgtccgcaga tgcacgtcgt
     2581 cgatcacctc acgggccagc ccacgggtga gacccgcaac gtcctcgtcg agtcggcgcg
     2641 gatcgcccgc ggcaaggtcg agccgctcga tcgcgcgcgc atggaggaac tcgacgcgct
     2701 cgtcctcccg gggggttacg gcgtcgccaa gaacctcagc gacttcgccg tccgcggccc
     2761 cgaggccgac gtcgagcccg agctcgtgcg gctggtcggc gaggccctct cgcgcgccaa
     2821 gccgctggtc gccatctgca tcagccccgc catcctcgcc gcggccctgc gcaagctcgg
     2881 catcggcggc gcccgcctca cgatcggcga cgacgacggc actgcggccg ccgtcaccgc
     2941 gctgggcgcc cgtcacgagg cctgcaccgt cgaccgcgcg atcgtcgacg agcgccatcg
     3001 catcatctcc acgccggcct acatgctcgg ccccggcccg cgcgacgtcg gcgccggcat
     3061 cgagcaggcg attgcgaccc tcgccggctg gctgcgctga gcgaccgggc ccgtgtaaca
     3121 ctcgccgggc cgcaccgttg ccgaggagtt cctgatgtcg cgccgcttcc cccgtgccct
     3181 cgtcttcctc ctcgccctcg cctccgcgtg tggcggcgag cccgtgaagt tggccgccaa
     3241 ggccgagaag ctcgaggtca agccgccgcc gcccgccccg accacggtgc acctcgccat
     3301 cgaaggcagc agcggcaagc tgggcttcga catggaggcc ccgatcgaga agatccgcgg
     3361 caaggtcccg gcctccgccc tgagcggcga agtgtggctc gacccgaccg acctcgccaa
     3421 gtcacgcggc ctcgtgcacg tcgatctccg cgacctcgag ctgttccagc gcaccgctcc
     3481 ggaggagggc aaggagttcg gcgaggaggt caagaacgac acgcagaacg agcacgcccg
     3541 ccagtggctc gagatctcgc ccgacacgcc cgccgacgag ctggccaaga acagcgtcgt
     3601 cgagttcgcc ctgacgcgga tcagcgacgc ctccgcgacc gacctgtcca aagtgacagg
     3661 tcccgaacgc aaggtcacct tcaccgccag cggcgacttc ttgctgcacc agcgcaaggc
     3721 cgagaagtcg gtcaagctcg aggccacctt ccgctacgac ggcgacgccc tgcgcgaggt
     3781 ccacgtcagg agcgtcgagc cgctggtaat cggcctcgac gagtacgacg tccgcccgcg
     3841 caccggtttc agcaagctgg ccgccaagac gctcgagcag ctcgccccca aggtcgccaa
     3901 ggaggccgcg gtctcgctcg agttcaccgc ccgacccgac ggcaagccgg cgatccccga
     3961 ggccgccgcg cccgccaatt ccggcccgcc cgcggccccg tcgcccgccc ctgccacccc
     4021 ttccgccccc gctgccgagc cggcccccgc ggccgcagcc aagtgaccga cgcgcggcga
     4081 tccgacgcgc gtccatcccc gtaacgaacg cacaggagca ctcaatcatg gatattcgca
     4141 cgatctccgc caccctcgcc ctgctcggct ctggcctcac tctcggcggc tgcaacaaga
     4201 cccagaccga cgccaccgag gtgcccggca acgccggcgc cgcgggcgag aagtcgtgca
     4261 agggccacgc ggacagcgag aagagctgcg gcggccacgc cgagggcgag aagagctgcg
     4321 gcggcgagaa gggctgcggc ggcgagaagg gctgcagcgg cgtgaagggc gacacgacct
     4381 gcgccgcctc gaagccgacc gaggccgccg agaccgcgcc ggccgccgac ccggccacca
     4441 ccgccactgc caccgcgccg gccgccaccg cggccgagcc gaccgccacc aagtccgaga
     4501 agaagaagaa gccggccgcc aagaaggcca agggcgaggc cgcctgcggc gagggcacct
     4561 gcggctgatc gccgcgcgga gcaacctgtc ccctcgacca gcccgagccg cgcgcccggg
     4621 ctggcccccc gtgacgctcg tgcgctcagt tcgatcgccc agcggctccc acgtccgtcg
     4681 catgtctgcg actgtcgtcc tctcgagcca tcgatgccgc acatcgtcgc gtccggacga
     4741 aaaaagctgt cctttcgacc agccgagctt ccggcatccc gtcgatgccg cacatcgtcg
     4801 cgttcgggcg cgacacaggc tgtcctttca accagccgac tctccggtat ccggaggtgc
     4861 ggcacatcgt cgcgttcggg cgcgacacag gttgtccttt cgaccagcca gccttccggc
     4921 atctcgtcga tgccgaccgc gccagtcggc gtcggccatc tcgacccgtc acctgcctga
     4981 cccgaggcgc catgcctgac gtgaagcgtc cgaccggcat cggcctcggc ctgcgctggg
     5041 acttcctcga cgaggctctg gctgcgcccg tcgatcctgc gctcgcgttc ttcgagatct
     5101 cgcccgagaa ctacatgcgc cgcggcggct acttcccggc cgctctcgag acgatcgccg
     5161 aacgacggcc gctgctgacg cacgggctca tgctcaacgt cggcgccgcc accggactgg
     5221 acgacgccta cctcgccgag ctgcgcagct tcctcgacgc gctccgcgtc gccgagcaca
     5281 ccgaccacct gtgctggacc ggccgcggcg gcgtgtgcct gcacgacctg ctgccgctgc
     5341 cgttcaccag ggccgcggct cgccaggtgg ccgaccagat ccgccgggcc caggacgccc
     5401 tcgcgcggcc gctcgcgctc gagaacatca gctattacgc ggccgccggc gcctcacgcg
     5461 ggtccgatcg cggcgcggcg ctggcagccc gcgagctcga gttcctgcac gaggtgctca
     5521 gccgcgccga cgccggtctg ctgctcgacg tcaacaacgt cgtcgtcaac gcgaccaacc
     5581 acggcttcga cccggacgcc ttcgtcgccg acctgccgct cgagcgggtc gtccgcctcc
     5641 acgtcgccgg cggcgaggtc cgcccgcacc tcggcggcct gctcatcgac acccacggcg
     5701 ccccggtgcc cccggccgtc ggcgcgctga tgggccgcgt cgtcgcccgc ctcggccccc
     5761 tccccgtcct gtacgagcgc gaccacaaca tccccgacta cgccaagctg gtggccgagg
     5821 tccgcgatct ggctggcatc tacgacgcgg ccctcgccgg cgagagcaga ccgaaagcac
     5881 atgtccccga ggacatccgc ctcacgggca cgagcgaggg gagctcgcac gggtccaact
     5941 ccggcgacga ggcagaagct cgccctgacg ccgtcctggc cgcgcgtccg cctgcagaac
     6001 ctcgcgattc gagcctggcc ggcccagctt ccgctgcacc tcacgacgac ctctccgcag
     6061 acgagcgcgc cgagctcgac gacatccagc ggggcgtctc gcatctcatc gtcgacctcg
     6121 aggccgagtc gcccgcgcgc cgcggtctgt ccgaattcct gcgtgcccgc ggcgtcggtc
     6181 cggcggcggt cgccgctttg gcggaggtcg acccggagcg gctgctcgtc tatcgccagc
     6241 tcatccgccg gcaggtgcgc ggcgtgctcg ccgacctgtt gtcgggcacc gcggccgccc
     6301 gcgggcaggt cgccttcgac gccgacgccg acgcctggat cgccgagctc ggcccccgct
     6361 cccgcctcct ccgcgacctg cccggcgagt tcgtcgcctg ggcgacacct cgctggtcga
     6421 acgaacatgt cctcgaggac ctgcccgaag cgccagcgtg gttgctggac ctcgcgcgcc
     6481 acgagctcct gacccacgaa gtcggcgcct ctccgccgcg ccccccgggc gacgagcgcc
     6541 cgttcgggct cgacgcccgc ctcgagttcg acccgagcgt ccgcctcggg cggttcgcgt
     6601 tcgcggtcca cgagcacccg gacgcgcccg acgtcgcgcc gcgccgcgag ccgaccgccc
     6661 tgctgctcta ccgcgaccgc gaacacgcgg tccgcaccct tcacctgagc ccgctcgccg
     6721 aggccttgct cgacgctctg ctgctgcgcg gcgtgacggt cgagcaggcc gtgcgggagg
     6781 ccgcggaggc gaccggcagc gcgatcgatg gcgacttcct gggccgcgtc agcgtgctgc
     6841 tcgccgacct atccgaacgc ggcgccatcc gcggcgcccg ctgaagccac gctcgccttc
     6901 agtgttccgg tcgcggtacc agcgatcccc ggcgagggcg aatcaccccg acgcccgagt
     6961 gctcgcgcac gggtcactcc gccgcccctg ctcgcgctcg cccgcatcga agatgccagg
     7021 ccggcatgtc tgcactttgg gacatgtccg aatcgacacg tcccacggac ctctgctcga
     7081 cgcttcggcc gcgctaccag tgcagcccga gctgcagcac gccctgcagc gcgtggccga
     7141 cttcccagcg cgccggcgtg atgccgtctt tcggcatgtt gagcgcgatc atccggtaca
     7201 ccgtggtgaa gccgagcgtc acctggttcg agacccaggc gtcaaagccg aggccggcgc
     7261 ggatctgggc cccgtgcagg acatcgccga gcggcagcct cgcgcgggcg tagccgacgc
     7321 cgagctccat cagcggctgg aagcgcttct tcatgaagta cagccggacg aacgccacgt
     7381 cccagaagaa catcccgccg ttcttgatgg tcttgtcggt ggtgccgggc agcgtcgtgc
     7441 ggatgctggt gtgcgtctga cccgagaggc ccgtgccgac gccgaggcgg ttgttgatcc
     7501 ggacgccgaa gtcgaacccg ccgccgagcg tcagcagcgt gggtcgggcc ggcttgtcgc
     7561 cgccgatcgg cccggtgatg gtgagccgcg gcgcgatcca aaaccggccg cgccacgagt
     7621 cgcgccggat cttcgacgga tcggctggag cgggcggcgg cggcagcggc tcgacctggc
     7681 gcccgcccca gtcgaatgag ggagcggcgc ccgcagccgg agccgggccc ggcggtgcgc
     7741 cggcggtagg tgcggtcgtc ggcggggccg cgaccggcgg tgagaccggg ggcggcgcgg
     7801 ccgagggcgg cggcaccgaa actccgctct cgggcgtcgg agccggcgtc gccaccgtct
     7861 cgccggcggg cgcgggaaca ggcgccggcg cggcagttgc gggagcaggt gccgacgcgg
     7921 caggtgcggg ctcctgcgcc agggccggcg tggcggccag cacgccggtg agacagatca
     7981 ggaccgcacg gatgtgagcg agcgtcattg ccggtgccct cgcgctaact tcggaagatc
     8041 tacaccacgc ggaacctctg cggcactcgg cggctttccg gacagcgccg atgaaagcgc
     8101 acgctccgcc tccgcgcgca actccggatc atcctcgttg gccgcggcga atcgcagaaa
     8161 accgacggca tctgccccgc ccaggcgcgc cagggctcga tacgcctcgc gcgtggcccc
     8221 agggtccagg cgatgcacct cgcgcacgat cgcggccgcg tggtcacgag tccccaccga
     8281 gccgaggacg tcgagcaccg ccatgcgcac ccgcatgtcg tcgctgcgca gcagcggcag
     8341 caggcgatgg gcgtgctccc tcgcctcgcg gtcgcccacg tagcggagcg cgaccaggat
     8401 ctgcgacggc tcgcccgacg acagcagcac gtcgagcccg ctcgcgtcgc cgcgggccag
     8461 gcgacactgc gcctcgagcc cggcgatcgc ccgccttgcc gcgtccgtca gcgcctccgt
     8521 cgacggccgg aacgcgtcgg tgccggccgt ggccgacgtc gtgagcgccg ccaccgcgaa
     8581 cttcgaacgg agatcgtcgg gcaccgccat gccgacgtgg aggacccagg gcgcgtcggc
     8641 ggcctcgcgg gtcagcgccg cctcgccctg gaccgtggag cccggcgcgc cgtccggcgc
     8701 gaagatcgcg ctgtcgcgca acgcgacctg gagcgcctcg gacaccggac cttcgaggtc
     8761 agcggcctcg ccgggcagcg ccaggctgag ccgcaccgga tagcgcccgc cgtccgattc
     8821 accgcacgcg gaggcgaaca ggacgaaggc gagcgccgcg cggagtcgga tcatcgtcga
     8881 tcgctaccat acccctctgc gccctcccag accagcggca ggcccaactg cgccgcgtag
     8941 agggccgcga agcgtcgccc ggccgcctcc actgtcgtct cccgcccgcc gaccgcgacc
     9001 gcgctggtca cggtcacgcc gtgcaagccg cacgacacga gcgcgccgaa cagcgccggg
     9061 tcgaccgcga ggttgaccga gatcccctgg accgtcaccc cgcggctcac gtggatcccg
     9121 atcgacgcca gcttctcggt tccgcgccac acgcccgggt gctccatgcg gaactcgcag
     9181 ccatcgaccc cgagctcgcc gaggaccgcg atcgccacgt cgccgaggcg cacgatgtgc
     9241 tcgcggatcc gccggccgat catcatgatc ggataggcga ccagctgacc cggcccgtgc
     9301 agcgtcacct cgccgccgcg cggcgcctcc cagatcgtga cgcccgcggc cgcgcgctgc
     9361 tcctccgacc acaggatgtc ctcccgccgg gcgcggcggc cgagcgtcag cacgggcggg
     9421 tgctcgacca ggaacagcac ccccggctcg cccgcgacca cgcgctcgcg cgcggccagc
     9481 tgcagcgcca gcgcctcttc gaacgccatg cggccgagcc acacggcgcg cagtgattca
     9541 ggtgtcgagg ccactgatcg actcgagcac ggtgggatcg agcgcgccga tcgccaccgg
     9601 caacgggctg atgccgacgc cctgggtgcg cagccagccg gcgagctcgc tcgcggtgac
     9661 gacccgggcg tcgccccacg gggcctccgc cggcgcatcg cccatgtaga acaccaggca
     9721 accgctggcc tgcgtccgct ccagatcgcc cgcccacgcc gacgcatcga tgcctgcgcg
     9781 ccgcggcagg atcagcgcca gcagcttcgg gttcgcctcg tcctcttcgg agtcggagtc
     9841 cgggtcaccc tcgatgtccc cgatcacgag cttggcgatc ccgcggctcg ggggcagcgt
     9901 cgccagcacc gggtagccct cgcgggtcag ccagatccgc acgaccgcct cgaggcctcg
     9961 cgggccaagt gtctgcaacc actgcacgaa cgcgttggcg atctcgttgt tgagcgccgt
    10021 gatcgcggcg tgcagcgcct gctcggcggc ggtcaccgcc gggggcacgc ggctcgcgtg
    10081 aagttgatag cgggcgtcgc cgcgggcgat gaaccgcgat gcccgaccgc cgtggcggat
    10141 gtcgagcagg atcgccgcag tcacggcgcg ctcgatctcc gagatctcgc cgccgagcac
    10201 gcccatgccg gccagggtct cggcgatctg gcggatgtgc agcgagcgcg ggccggcgtc
    10261 ggtcatcacc tgcgcgacct gctgcagcag cgactccgag gcctcgtcgc gcatcgtgcg
    10321 gatctcggcc acaccgccct cgttgcggcg accacggcgg cgacgacggc ggcggcggcg
    10381 gcccgggcga tcctcgtcct cactggcggc ttgcggctgg acctgcggtt gaggctgagg
    10441 ccgcggcggc ggcggcggtg cgccgatcgg ccgtcgcaga ctgcggtgtt cgacggccgg
    10501 cgcgctcgga gggggcggcg gcggcgggcg ctgcatgctc tcgagctccg tgcgcagctc
    10561 ggggcgctcc tcggccagcc gcgacatcgc gttcgccatc accacggcca cgtctgtcac
    10621 gccgtcgagg cccgcgccct tgggcgcctc gaactcgagg ttgggcgtca cgcgctggat
    10681 cacctcggcg cgcttgccgg tgtcgagccc gcggcgcgtc ggcttgatcg cgatctgctg
    10741 cggcgccgga gcctcgccgc ggacgccgaa ccgatctcgc cgccggcgcc ggcgacgacc
    10801 gccgccctcc tcggcctgac cctcgtcggc ttcgctcgtc tcttcctcgt cacgggtgtc
    10861 ttcggtcagc atcgaattcg cttcaacttt tggcggcaag gcctcgacat cgttcgattt
    10921 ctttgcactg acaggtgccg tctcgaaggg cagctcgagg gtcggcggcg gggtcggcgg
    10981 ggcggccggc gggggcggcg gctcggggac cggaggagcc ggagccggcg gcggcgctgc
    11041 gccgggccgg agctggaaga accccggctt gctccgcacc agcagggcgc tgtcgccacg
    11101 cacggccgcg ttgaggctgg cctgcatcgc cgcgaccgga tcgcggccca cgtgcgagag
    11161 cagctggcga tcgacggctc gcttcacgat ctcggagaaa tgcaggggtt cggactcgtg
    11221 gcggaggact tcgatcgcag cttcaaggaa ggtcatgtgt acgactgcgg cgcgccgggc
    11281 gacggcgacg gagatttgtg cagaaaattt gcgtggagag gtcggagtct gctagccgcg
    11341 gacgacatgc gtgcgcacaa cgtcggcgtc ctgatctcga ccgccaccgc tcgctcggtg
    11401 ggcgcggaca atgatagcca aatcgaccgc gaaagtggca gcgatcttgc ggaagcgctg
    11461 ggccggctcg gcctcgtgcc cgtctcgctg accgccgacg acgcgctcga cctcaccctg
    11521 cggcggaccc gcctgcgcgc ctgcttgctc gccgcccacg gcctcgccgg cgggctcggc
    11581 cggatccagg ccctgctcga gctgcgcaag atcccgttcg tcggcccgtc cgcgcagacc
    11641 accggcctcg cctacgacaa gctgcgcgcg cggcagatcc tggcctatca cagcctcccc
    11701 gtgccgacca cgctggccct gggcggcgcc gaacccccga gccggcacgc catcggcatg
    11761 ctcggctggc cctgcgtcct caagccgcgt cgcggcagcc acgcggccgg cgtccgcctg
    11821 ctcgccgacg cggacgccgt cgcggacgcc acccaggccg cgggtgacga ggccggcgag
    11881 ttct
//
