LOCUS       NZ_CP114040            10882 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Nannocystis poenicansa strain FL3 chromosome, complete genome.
ACCESSION   NZ_CP114040
VERSION     NZ_CP114040.1
KEYWORDS    RefSeq.
SOURCE      Nannocystis poenicansa
  ORGANISM  Nannocystis poenicansa
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Minimal conservation of predation-associated metabolite biosynthetic
            gene clusters underscores biosynthetic potential of Myxococcota
            including descriptions for ten novel species: Archangium lansinium
            sp. nov., Myxococcus landrumus sp. nov., Nannocystis bai
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2022) Biomolecular Sciences, University of
            Mississippi, Faser Hall 407, Oxford, MS 38655, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: IPA v. 10.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: PacBio Sequel IIe
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026965555.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 12:45:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,229
            CDSs (total)                      :: 10,129
            Genes (coding)                    :: 10,068
            CDSs (with protein)               :: 10,068
            Genes (RNA)                       :: 100
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 9 of 61
            Pseudo Genes (incomplete)         :: 52 of 61
            Pseudo Genes (internal stop)      :: 2 of 61
            Pseudo Genes (multiple problems)  :: 2 of 61
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:28:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5336263
            Orig. end                         :: 5347145
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10882
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10882
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10882
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5882](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5882)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            277..1566
                     /locus_tag="O0S08_RS20615"
                     /old_locus_tag="O0S08_20615"
     CDS             277..1566
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024140.5"
                     /locus_tag="O0S08_RS20615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_20615"
                     /product="HAMP domain-containing protein"
                     /protein_id="WP_269040912.1"
                     /transl_table=11
                     /translation="MTLRTRLLLGYGYLIALLLLSVGTAAFNSQRVARNVQVVVQNNLV
                     SLSAAVAMLDQIERQDSATLVGLMQRSEPTEMTAAEEDFEKALVQARSASMSDDDIAML
                     KNLQENYEVYRKARDELLRSIPENPYAAYHETVFRVFEELKRDVNQYLEAKKTALGDAN
                     IEASQSAIEAAVLLGVLVTVALLSLGPLSRSLQRDVLGRLSEVGSVTEAIASGDRRRRV
                     RDFYQDELGQVARQLNAALDRQQELESQMQGRLLEQRRVLVGLMKQWPMPAALIGIDGE
                     LVASTLSEDDEAELDRLTPRVRMAAKVLMTRRFVRAEELSADIKASDGVRVVQMRALAS
                     GDNQIAGWLASFVDPRVGVLAPRGPGDLAPSDESSETGEVPASEEVESGGDEAPASDVA
                     SDEARENDEPALADASVAPTADEESEQRPV"
     gene            1610..2749
                     /locus_tag="O0S08_RS20620"
                     /old_locus_tag="O0S08_20620"
     CDS             1610..2749
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013324.5"
                     /locus_tag="O0S08_RS20620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_20620"
                     /product="SPFH domain-containing protein"
                     /protein_id="WP_269040913.1"
                     /transl_table=11
                     /translation="MAQQRKSGRPSGGQILGDLQGKLKSVTGGGGSSWFDWVWIGGALI
                     AGLVLMTTSFTAIEPGQVAVRINNITGGLSTVTQPGWITRLPFGIHSVYVLDASPQTFS
                     MHGSKTIDDLEVSELTVRASDGSNFHFSDTTIIFQIAGDEAQTVIRDGGEERGYLKWMR
                     PYARAILRDEFGRESTISVSNPTKFGEATERAKSRLNELLGVHGVIVTQIVTPRPQFND
                     EYEKLIEERNKLGNQLEVIKSNLAAAETSRERRLAEVNRDQNRQIQEKRTELESALATA
                     IAAQADATREADTYKISKVAEGQATLSASKQQAAELRGQLDAVYRARKAEIDAFRSQPV
                     ERVMERLGERLRGVTIAIEPWANDANPSRVEVEKVGGAQ"
     gene            2746..3957
                     /locus_tag="O0S08_RS20625"
                     /old_locus_tag="O0S08_20625"
     CDS             2746..3957
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013324.5"
                     /locus_tag="O0S08_RS20625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_20625"
                     /product="SPFH domain-containing protein"
                     /protein_id="WP_269040914.1"
                     /transl_table=11
                     /translation="MKGFVRICQLTLLSLLFLWIAPTCFISTVPLGTIGVRSSNASGVL
                     AADLEPGWTLNMAGIHSLTLLPSHYLFLDYGSEEGQGLQIRTRDNNNVFVDVSVPYRIK
                     PGEAHQIMMAGNHVESTNGQYRFQRLAQETTVSVLREGLANLTSSDFYHADTRLKVADD
                     TLARLNQELAPLHLEAQAVLVRAVTFRDEYEKQLQQIQLNEQNKLLDGAREKVAKQQQE
                     LDNYELGTNALANARQQDWIKRQADLERAYQVGFVDTKGDSTPGSARRALKALSDEERA
                     ALRVEVGKVLDIDAPEAIADGYLLGIKNIEAETLEYRQRVNAEADGVSQRLAAEGAADV
                     AAVQGEFESKINALLGSPAGRAYVAYKSAENVRFAETLTFQSTDGIPAVLRLRDFALRF
                     MGGG"
     gene            complement(4240..5004)
                     /locus_tag="O0S08_RS20630"
                     /old_locus_tag="O0S08_20630"
     CDS             complement(4240..5004)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS20630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_20630"
                     /product="hypothetical protein"
                     /protein_id="WP_269040915.1"
                     /transl_table=11
                     /translation="MSRLDPRRVQAALVCMHLDPAYAAAVRGPGPLPELADRERELLRA
                     VDPRALAVDPYRRARAVHALLEEYPATAALLGVPAVDAFFSSAAFRTCAFEHGSMALSF
                     GTWLDDHAGGPGRIEAAIARARRPRAGAGEGLACNPCVAPLLVPAGSLAFLERVRARLG
                     PDPAAALANARPSRERPPRGRDREPLLVEAGPGGEVSLGTASEPLVRLLLLAGDGRPHA
                     ALAAEAVRLGAEPHEADELLAGLVADGLLVAC"
     gene            complement(5001..5882)
                     /locus_tag="O0S08_RS20635"
                     /old_locus_tag="O0S08_20635"
     CDS             complement(5001..5882)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.5"
                     /locus_tag="O0S08_RS20635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_20635"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_269040916.1"
                     /sec_met_domain="DUF692 (E-value: 5.3e-18, bitscore: 64.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDFLARVRALPRLGLGVSTEYGARRQDGALDPLELRRRRPDCAG
                     FLEVGVEVVKGLDADARAWAAGGLPTTYHFLDINLDNPDDFDPPWLEGVRTIAAELRPA
                     WLCGDAGLWHLGRRDRGHMLLLPPVLVDEAVAPLADGLIALREATGLEVLPENPPGAAF
                     VGELHLLDFFARVAERADTGLLLDCAHLAMFQRARGHAPLTGFDGFPWDRVVELHVAGG
                     RVQDTAGLAWVEDDHGTDVLPDTWEIAAEAVARAVNLRAIVVECERNPIDAVLPLFDRV
                     AALWPGRGPEAA"
     misc_feature    5947..5958
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.12"
                     /tool="antismash"
     gene            complement(5954..6577)
                     /locus_tag="O0S08_RS20640"
                     /old_locus_tag="O0S08_20640"
     CDS             complement(5954..6577)
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /GO_process="GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_N_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1193: glutathione S-transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739613.1"
                     /locus_tag="O0S08_RS20640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_20640"
                     /product="glutathione S-transferase family protein"
                     /protein_id="WP_269040917.1"
                     /sec_met_domain="GST_N_3 (E-value: 9.8e-15, bitscore: 53.9,
                     seeds: 52, tool: rule-based-clusters)"
                     /sec_met_domain="GST_C (E-value: 2.2e-12, bitscore: 46.2,
                     seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSITFYYAPNTSASRVHWVLEELGIPYDKVKLDLRAGEQHKPEFL
                     AINPNAKVPALVVDGTPMFESLAIIIYLGDRFGVEKGLWPRVGSGEHAEALAWSVWGSV
                     TLGGTIFRLFYNTSEWFPAESRNAPQAETARKEFEAQLGILDQRLRGREYLVGDHFTLV
                     DAANAAAIAWALSFIKVDTTRLPDLSAWLERSTQRPANQAVHAG"
     gene            complement(6647..8512)
                     /locus_tag="O0S08_RS20645"
                     /old_locus_tag="O0S08_20645"
     CDS             complement(6647..8512)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015983.5"
                     /locus_tag="O0S08_RS20645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_20645"
                     /product="radical SAM protein"
                     /protein_id="WP_269040918.1"
                     /sec_met_domain="PF04055 (E-value: 4.9e-18, bitscore: 65.3,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDDRTHARPRTSRPPRLSPGDYKALIKVGYGCNEHCTFCHTLDVR
                     PIDGDAEEVERKILRAAELGHTMIVLSGGEPTIRPELLRWATQVAGLGLDLGLVTNGRA
                     LAYAELTDKLVANRLRYVYLSLHGGSAPVHNRLVRSDAFDQAVAALRNLSGRGLDLTVN
                     CVVTRQNLAHLRGLVDLVAAFADVTLKFSMVEPKGGAARNFELLVPRVGAVAEAVLAAA
                     EHGRERGVRVQHGGIPRCLVPGLEDSTSDLRSHRYWTMVEIGEPDLFPVDDDNKRHPPA
                     CDGCSLRGGCPGLYRGYHEVHGDGELRPVTDRPRANSFNYTLEAVHEAPEATCPLRQGP
                     LGVTPWERGRHLFVRRGGAIARYRADTRDFTDEEIAAVKHDLGQVYVDAARGPAPQDFA
                     RELVPLARSPLCAGCPHLSACTGMFEPVFEDIFTRDDAVVRARLAGLAGDVLDLGCGDA
                     PYLDVLAPRIAAGAIRYTGVDPDAAALARLRARLPPGAALERGDAEALELGERRFDAVT
                     ILRSFNHLRDPARVLSTALSRLRPGGLLVVCDNTAFGLARTPAQTTRGERSTARREHYR
                     NDTAADAARRIEAVAAELGVTLASHDRRDIGPGTSNQWLLVYSLA"
     gene            8708..9196
                     /locus_tag="O0S08_RS20650"
                     /old_locus_tag="O0S08_20650"
     CDS             8708..9196
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS20650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_20650"
                     /product="hypothetical protein"
                     /protein_id="WP_269040919.1"
                     /transl_table=11
                     /translation="MKPSPRVARRKVLAALGVAAAGVSTAACTLRPRTTAPSAAAPLAD
                     LVGPVGEWRVEQVLPLVAGAVPVLMRTPEGRKFQVDLLRAEVDGPRAVAQAGDLALYLA
                     NGGAGDRPSTAVEEQGARALAQRLSQLAAERGAPAIPGLLTLSERLASHRQAVLTPWS"
     misc_feature    9316..9334
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.87"
                     /tool="antismash"
     gene            9387..10223
                     /locus_tag="O0S08_RS20655"
                     /old_locus_tag="O0S08_20655"
     CDS             9387..10223
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS20655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_20655"
                     /product="hypothetical protein"
                     /protein_id="WP_269040920.1"
                     /transl_table=11
                     /translation="MTLARAVGVDPSGPEGQGVLNMLRGAGGTLTLAGADGEAIAAWIA
                     AARGAGFARVVAAPGPAGLAAEDLARLKTAGFDGLRVHLYAAESAGHDYHAGSEGSFRA
                     TTAMLRAARTVPLPVTVTTPLTRSNTRVLAAMPLLLADVGARAWQIEVVAEGRPDGYGA
                     VAPRLAVAVPYALQAMVAAERAGLMAAIAGAPLCLLGPLRERAVPGPARAFADGCSDCA
                     SRANCAGVDPEYLRRFGPGELAPVRGGEAEVGAAQRLQDMFSGPCLEVSEGACWPI"
     gene            10208..10402
                     /locus_tag="O0S08_RS20660"
                     /old_locus_tag="O0S08_20660"
     CDS             10208..10402
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS20660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_20660"
                     /product="hypothetical protein"
                     /protein_id="WP_269040921.1"
                     /transl_table=11
                     /translation="MLADLTPPAPGSETARGLFSGPWLEVFGGRVLADPTLPAPGSETV
                     RGLFSGPWLEVSEGACWPI"
     misc_feature    10864..10873
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.13"
                     /tool="antismash"
     misc_feature    complement(10865..10878)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.83"
                     /tool="antismash"
     misc_feature    10866..10879
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.23"
                     /tool="antismash"
     misc_feature    10867..10880
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.18"
                     /tool="antismash"
ORIGIN
        1 gcccacccga ccatcctcga gcagatcgcg ctcgacgaga acaccaacct ggtcgaggtt
       61 gcgacgcccg aacaattcat cggcaagacg atggcggaga tcgacatgaa gcggcgctac
      121 ggggtgtcgg tgatcgcggt ccggcgcggg gggcaggtca tccctcagct cgccgtcgac
      181 gaccggctcg cccgcggcga tcgcctgctc atcgtcggcc cgccggcgtc ggtccgccgc
      241 gtcgccagcc tggcgtagac ccccggaacc gccgccatga ccctccgcac ccgcctcctg
      301 ctcggctacg gctacctgat cgcgctgctc ctgctcagcg tcggcacggc cgcgttcaac
      361 tcccagcggg tcgcccgcaa cgtgcaggtg gtcgtgcaga acaacctggt cagcctgagc
      421 gcggcggtgg cgatgctcga ccagatcgag cgccaggaca gcgcgacgct ggtcggtctg
      481 atgcagcgca gcgagccgac cgagatgacg gcggccgagg aggacttcga gaaggcgctg
      541 gtccaggcgc gcagcgcgtc gatgtccgac gacgacatcg ccatgctgaa gaacctccag
      601 gagaactacg aggtctaccg caaggcgcgc gacgagctgc tgcgcagcat ccccgagaac
      661 ccgtacgcgg cctaccacga gacggtcttc cgcgtgttcg aggagctgaa gcgcgacgtc
      721 aatcagtacc tcgaggcgaa gaagacggcg ctcggcgacg ccaacatcga ggcgtcgcag
      781 tcggccatcg aggcggcggt gctgctcggc gtgctcgtca ccgtggcgct gctgtcgctc
      841 gggccgctgt cccgcagcct gcagcgcgac gtgctcggac gcctgtcgga ggtcggcagc
      901 gtgaccgagg cgatcgccag cggcgaccgc cgccgtcgcg tgcgcgactt ctatcaggac
      961 gagctgggcc aggtcgcgcg ccagctcaac gcggcgctcg atcgccagca agagctcgag
     1021 tcgcagatgc agggccgact gctcgagcag cgccgcgtgc tggtcggctt gatgaagcag
     1081 tggccgatgc ccgcggccct gatcggcatc gacggcgagc tggtggcgtc gacgctcagc
     1141 gaggacgacg aggccgagct cgatcgcctg acgccgcgcg tgcgcatggc ggcgaaggtg
     1201 ctgatgacgc gccgcttcgt ccgcgccgag gagctgtcgg ccgacatcaa ggcgagcgac
     1261 ggcgtacgcg tggtgcagat gcgcgcgctg gcctccgggg acaaccagat cgcgggctgg
     1321 ctggcgtcct tcgtcgatcc gcgggtcggg gtgctggcgc cgcgcgggcc gggcgatctc
     1381 gcgccgagcg acgagtcgag cgagacgggc gaggtgccgg cgagcgaaga agtggagagc
     1441 ggcggcgacg aggcgcccgc gagcgacgtg gcgagcgacg aggcgcgcga gaacgacgaa
     1501 cccgcgctcg cggacgcgtc agtggcgccg acggccgacg aggagtccga gcagcggcct
     1561 gtctgagccg gctcgcgggg cctcgcgggg cctggtgtac gatgcgggca tggcgcaaca
     1621 gcggaaatca ggccggccca gcggcggcca gatcctggga gacctccagg ggaagctcaa
     1681 atcggtgacc ggcggcggag gctcgagctg gttcgactgg gtgtggatcg gcggggcgct
     1741 gatcgccggg ctcgtcctga tgacgacctc gttcacggcg atcgaacccg gccaggtggc
     1801 ggtgcggatc aacaacatca ccggcgggct gtcgacggtg acgcagccgg gctggatcac
     1861 gcggctgccg ttcgggatcc actcggtcta cgtgctcgac gcgagcccgc agacgttctc
     1921 gatgcacggg tcgaagacga tcgacgacct cgaggtcagc gagctgacgg tgcgcgccag
     1981 cgacggctcg aactttcact tctccgacac cacgatcatc ttccagatcg ccggtgacga
     2041 ggcccagacg gtcatccgcg acggcggcga ggagcgcggc tacctcaagt ggatgcgccc
     2101 gtacgcccgg gcgatcctgc gcgacgagtt cggccgcgag tcgacgatct ccgtgtccaa
     2161 cccgaccaag ttcggcgagg cgaccgagcg cgccaagagc cggctcaacg agctgctcgg
     2221 ggtgcacggc gtgatcgtga cccagatcgt gacgccgcgg ccgcagttca acgacgagta
     2281 cgagaagctg atcgaggagc gcaacaagct cggcaaccag ctcgaggtca tcaagtccaa
     2341 cctcgcggct gccgagacca gccgcgagcg ccggctcgcc gaggtcaacc gcgatcagaa
     2401 ccgccagatc caggagaagc ggaccgagct cgagtcggcg ctggcgaccg cgatcgcggc
     2461 ccaggccgac gcgacccgcg aggccgacac ctacaagatc tcgaaggtcg ccgagggcca
     2521 ggcgacgctc agcgcgtcca agcagcaggc ggccgagctc cgcggccagc tcgacgcggt
     2581 ctaccgcgcg cgcaaggccg agatcgacgc gttccgctcg cagccggtcg agcgcgtgat
     2641 ggagcgcctg ggcgagcgcc tgcgcggggt gacgatcgcg atcgagccgt gggccaacga
     2701 cgccaacccg tcgcgggtcg aggtcgagaa ggtcggaggt gcgcagtgaa gggcttcgtc
     2761 cggatctgtc aattgacgct gctgtcgctg ctgttcctgt ggatcgcgcc gacgtgcttc
     2821 atctcgacgg tcccgctcgg cacgatcggg gtccgcagca gcaatgcgtc gggcgtgctc
     2881 gcggcggacc tcgagcccgg gtggacgctc aacatggcgg ggatccactc gctgacgctg
     2941 ctgccgagcc actacctgtt cctcgactac ggctcggagg agggccaggg gctgcagatc
     3001 cgtacgcgcg acaacaacaa cgtgttcgtc gacgtcagcg tgccgtaccg catcaagccc
     3061 ggcgaggcgc atcagatcat gatggccggt aaccacgtcg agagcacgaa cgggcagtac
     3121 cggttccagc ggctggcgca ggagacgacg gtgagcgtgc tgcgcgaggg cctggccaac
     3181 ctgacgagct cggacttcta ccacgccgac acgcggctca aggtggcgga cgatactctg
     3241 gcgcggctca accaggagct ggcgccgctg cacctcgagg cgcaggcggt cctggtgcgg
     3301 gcggtgacgt tccgcgacga gtacgagaag cagctgcagc agatccagct caacgagcag
     3361 aacaagctgc tcgacggggc gcgcgagaag gtcgccaagc agcagcagga gctcgacaac
     3421 tacgagctcg gcaccaacgc gctggccaac gcgcggcagc aggactggat caagcgccag
     3481 gccgacctcg agcgcgcgta ccaggtcggc ttcgtcgaca ccaagggcga cagcacgccg
     3541 ggctcggccc gccgcgcgct caaggcgttg tccgacgagg agcgggccgc gctgcgcgtc
     3601 gaggtcggga aggtgctcga catcgacgcg cccgaggcga tcgccgacgg ctacctgctc
     3661 gggatcaaga acatcgaggc cgagacgctc gagtaccgcc agcgcgtgaa cgcggaggcc
     3721 gacggcgtgt cgcagcgcct ggcggccgag ggcgcggccg acgtcgctgc ggtccagggc
     3781 gagttcgaga gcaagatcaa cgcgctgctc ggcagcccgg ccggtcgcgc ctacgtcgcc
     3841 tacaagagcg cggagaacgt gcgcttcgcc gagacgctga cgttccagag caccgacggg
     3901 atcccggcgg tgctgcgcct gcgagacttc gcgctgcgct tcatgggcgg cggctgagtt
     3961 tttttcaaag agaaaagaag aattgagtgg caagcgacgc gcgacctcgt gggcggggcg
     4021 cgcgtcgctc gtcgcgttgg gtcgaggggt cgtgagcgac gcacggcctc ggcgatgagg
     4081 cgcgcgtcgt caggttgttc gagcgagtcg tgggcgatgc gcgacctcgg cggcgaggta
     4141 cgcgtcgctc gtcgcattgc ttcgagtgcg tcgtgagcga cgcacggcct cggcgagggg
     4201 gcgctcgtcg cgtcgctccc gctgggtcgt aggcgatgct cagcaggcga ccagcaggcc
     4261 gtcggcgacg aggccggcca gcagctcgtc ggcttcgtgc ggctcggcgc cgaggcgcac
     4321 ggcctcggcg gcgagagcgg cgtggggtcg gccgtcgccg gcgagcagca gcagacggac
     4381 gagcggctcg ctggctgtcc cgagggacac ctcgccgccg ggaccggctt ctaccagcag
     4441 gggttcgcgg tcgcggccgc gcggcgggcg ttcgcgcgac gggcgggcgt tcgccagcgc
     4501 tgcggcgggg tcggggccga ggcgggcgcg cacgcgctcg aggaaggcca ggctgccggc
     4561 ggggaccagc agcggggcga cgcacgggtt gcaggccagg ccctcgccgg cgccggcgcg
     4621 cgggcggcgg gcgcgggcga tggcggcctc gatgcggccg gggccgccgg catggtcgtc
     4681 gagccatgtc ccgaaggaca gggccatcga gccatgttcg aacgcgcagg ttcggaacgc
     4741 ggccgacgag aagaaggcgt cgacggcggg cacgccgagc agggcggcgg tggccgggta
     4801 ctcctcgagc agggcgtgca cggcgcgggc gcggcggtac gggtcgacgg cgagggcccg
     4861 cggatcgacg gcgcgcagca gctcgcgctc gcggtcggcg agctcgggca gcggacctgg
     4921 cccgcgcacg gccgcggcgt aggcggggtc gaggtgcatg cagaccaggg cggcctgcac
     4981 ccggcgcggg tcgaggcggc tcacgcggcc tccgggccgc gccccggcca cagcgcggcg
     5041 acgcggtcga acagcggcag gacggcgtcg atcgggttgc gctcgcactc gacgacgatc
     5101 gcgcgcaggt tgacggcccg cgccaccgcc tcggcggcga tctcccaggt gtccggaagg
     5161 acatccgtgc cgtggtcgtc ctcgacccat gcgaggcccg cggtgtcctg cacgcgcccg
     5221 ccggcgacgt gcagctcgac cacgcggtcc cacgggaagc cgtcgaagcc ggtgagcggg
     5281 gcgtggccgc gggcgcgctg gaacatggcc aggtgggcgc agtcgaggag caggccggtg
     5341 tcggcgcgct cggcgacgcg ggcgaagaag tcgagcaggt gcagctcgcc gacgaacgcg
     5401 gcgccgggcg ggttctcggg caggacctcg aggccggtcg cctcgcgcag cgcgataagg
     5461 ccgtcggcca gcggcgcgac ggcctcgtcg acgaggaccg gcggcaggag cagcatgtgc
     5521 ccgcggtcgc ggcggccgag gtgccacagg ccggcgtcgc cgcacagcca cgccgggcgc
     5581 agctcggcgg cgatcgtgcg caccccctcc agccacggcg ggtcgaagtc gtcggggttg
     5641 tcgaggttga tgtcgaggaa gtggtaggtg gtcggcaggc cgccggcggc ccaggcccgc
     5701 gcgtcggcgt cgaggccctt gaccacctcg acgccgacct cgaggaagcc ggcgcagtcg
     5761 ggccggcgcc gccgcagctc gagcgggtcg agggcgccgt cctgccgccg cgcgccgtac
     5821 tcggtgctga cgccgaggcc gaggcgaggg agggcgcgga cgcgggcgag gaagtcgggc
     5881 atggacaggt ccctgggaca ggtccgaacg ggcaggtcgg aaaggacctg accgttcgga
     5941 catggcgaaa aggctagccg gcgtggaccg cctggttcgc cgggcgctgg gtgctgcgct
     6001 ccagccaggc ggacaggtcg gggaggcggg tggtgtcgac cttgatgaag ctcagggccc
     6061 aggcgatcgc ggcggcgttg gcggcgtcga cgagggtgaa gtggtcgccg accaggtact
     6121 cgcggccgcg caggcgctgg tcgaggatcc cgagctgggc ctcgaactcc ttgcgggcgg
     6181 tctccgcctg gggggcgttg cgcgactcgg ccgggaacca ctcgctggtg ttgtagaaga
     6241 ggcggaagat cgtgccgccg agagtgaccg agccccacac gctccacgcc agcgcctcgg
     6301 cgtgctcgcc ggagccgacg cgcggccaca ggcccttctc gacgccgaag cggtcgccga
     6361 ggtagatgat gatcgccagc gactcgaaca tcggcgtgcc gtcgaccacg agggcgggca
     6421 ccttggcgtt ggggttgatg gccaggaact ccggcttgtg ctgctcgccg gcgcggaggt
     6481 cgagcttgac cttgtcgtag gggatcccga gctcctcgag gacccagtgc acgcgcgagg
     6541 cgctggtatt gggggcgtag tagaaggtga tcgacatcgg actccgcggg ttatgtacgc
     6601 gatcgggcag cgatgcggta ggcgagcgca gcggtcggcg gggagctcag gcgaggctgt
     6661 agacgagcag ccactggttg ctggtgcccg gcccgatgtc gcggcgatcg tgcgacgcga
     6721 gggtcacgcc gagctcggcc gcgacggcct cgatccggcg ggcggcgtcg gcggcggtgt
     6781 cgttgcggta gtgctcgcgg cgggcggtcg agcgctcgcc gcgggtggtc tgcgcgggcg
     6841 tgcgggccag gccgaaggcg gtgttgtcgc agacgacgag gaggccgccg gggcgcagcc
     6901 gcgagagggc cgtcgagagc acgcgcgcgg ggtcgcggag gtggttgaag ctgcgcagga
     6961 tcgtgaccgc gtcgaaccgg cgctcgccga gttcgagcgc ctcggcgtcg ccacgctcga
     7021 gcgctgcgcc gggcggcagg cgagcgcgca ggcgggccag agcggcggcg tcggggtcga
     7081 cgccggtgta gcggatcgcg ccggcggcga tccgcggggc caggacgtcg aggtaggggg
     7141 cgtcgccgca gccgaggtcg aggacgtcgc cggcgagccc ggccagccgc gcgcgcacga
     7201 cggcgtcgtc gcgcgtgaag atgtcctcga agacaggttc gaacatccct gtgcatgcgg
     7261 acaggtgcgg acagccggcg cacagcgggc tgcgggccag cgggaccagc tcgcgggcga
     7321 agtcctgggg ggccgggccg cgggcggcgt cgacgtagac ctggccgagg tcgtgcttga
     7381 ccgcggcgat ctcctcgtcg gtgaagtcgc gggtgtcggc gcggtagcgg gcgatcgcgc
     7441 cgccgcggcg gacgaacagg tggcggccgc gctcccacgg ggtgacgccg agggggccct
     7501 ggcgcaaggg acaggtggcc tcgggggcct cgtgcacggc ctccagcgtg tagttgaacg
     7561 agttggcgcg cgggcgatcg gtgaccgggc gcagctcgcc gtcgccgtgg acctcgtggt
     7621 agccgcggta gaggccgggg cagccgccgc gcaggctgca gccgtcgcac gccggcgggt
     7681 ggcgcttgtt gtcgtcgtcg accgggaaca ggtcgggctc gccgatctcg accatggtcc
     7741 agtagcggtg gctgcggagg tcgctggtgc tgtcctcgag gccaggtacg aggcagcgcg
     7801 ggatcccgcc gtgctggacc cgcacgccgc gctcgcgccc gtgctcggcg gcggcgagga
     7861 cggcctcggc gacggcgccg acgcgcggga ccagcagctc gaagttgcgc gcggcgccgc
     7921 ccttgggctc gaccatcgag aacttgaggg tgacgtcggc gaacgcggcg acgaggtcga
     7981 cgaggccgcg caggtgggcg aggttttggc gggtgacgac gcagttgacg gtgaggtcga
     8041 ggccgcggcc gctgaggttg cgcagggcgg cgacggcctg atcgaaggcg tcgctgcgga
     8101 ccaggcggtt gtgcacgggg gcgctgccgc cgtgcagcga gaggtagacg tagcgcaggc
     8161 ggttggcgac gagcttgtcg gtgagctcgg cgtaggcgag ggcgcggccg ttggtgacga
     8221 ggccgaggtc gaggccgagg ccggcgacct gcgtggccca gcgcaggagc tcggggcgga
     8281 tcgtcggctc gccgccggag aggacgatca tggtgtgtcc cagctcggcg gcgcggagga
     8341 tcttgcgctc gacctcctcg gcgtcgccgt cgatcgggcg cacgtcgagg gtgtgacaaa
     8401 aggtgcagtg ctcgttgcag ccgtagccga ccttgatcag cgccttgtag tccccgggcg
     8461 agagcctcgg cggccgcgag gtccgcggtc gggcgtgggt gcggtcgtcc atgcgcagcg
     8521 ccgatgctag cgcgtcctcg cggcggtcgc cgagagtcga acgcggccgg gcggagcgag
     8581 ggccggtgcg gcggggccgg cgagcgtcgg aagccgagcg tcggcggtcg tgaggggtcg
     8641 gcgctcgcgt cgccttgacg ggggctgcgg cgggcgggta cgttgggctc cgattaggaa
     8701 agactgcatg aagccttcgc cgcgtgtcgc ccgtcgcaag gtcctggctg ccctcggagt
     8761 cgccgctgcc ggcgtgtcga cggcagcctg cacgctgagg ccccgcacga cggcgccgtc
     8821 ggccgcggcc ccgctggccg atctggtcgg gccggtcggc gagtggcgcg tcgagcaggt
     8881 gctcccgctg gtggccggcg cagtgccggt gctgatgcgc acgccggagg gccggaagtt
     8941 ccaggtcgat ctgttgcgcg cagaggtcga cggcccacgc gcggtcgcac aggccggcga
     9001 cctggcgctg tacctcgcca acggcggcgc gggcgaccgc ccgtcgaccg ccgtcgagga
     9061 gcagggcgcg cgcgcgctgg cgcagcggct gtcgcagctc gcggccgagc gcggggcgcc
     9121 ggccatcccc gggctcctga cgctgtccga gcgcctggcg tcgcaccgcc aggccgtgct
     9181 gacgccgtgg agctgagcgg ccgcgggacg gtgcccggtc ccggaggaca gggcgcttcg
     9241 aacatgtcgc gtgagacagg tgatcctgtc ctggggggac aggtgatcat gagcgcggcg
     9301 cgcggacggg tacctttctg tctgaaagga caggctgcac cgagcccgtg cggcgcaggg
     9361 gattctgtcc ggaaggacat gtccaagtga cgctggcgcg cgcggtgggg gtcgatccgt
     9421 ccggtccgga aggacagggc gttttgaaca tgttgcgagg ggcaggtggg acgctgacgt
     9481 tggcgggcgc cgacggcgag gcgatcgcgg cctggatcgc ggcggcgcgg ggggcagggt
     9541 tcgcgcgggt ggtggccgcg ccggggccgg cagggctggc ggccgaggat ctggcgcggc
     9601 tgaagaccgc cgggttcgac gggctgcgcg tgcatctgta cgcggccgag tcggcggggc
     9661 acgactacca cgcgggtagc gaggggtcgt tccgggcgac gacggcgatg ctccgcgcgg
     9721 cccggacggt cccgttgccg gtgaccgtga cgacgccgct gacgcgctcc aacacgcggg
     9781 tgctggcagc gatgccgctg ctgctcgcgg acgtgggggc gcgcgcgtgg cagatcgagg
     9841 tggtcgcgga gggccgcccg gacgggtacg gggcggtggc gccgcggctg gcggtggcgg
     9901 tgccgtacgc gctgcaggcg atggtggcgg ccgagcgggc ggggctgatg gcggcgatcg
     9961 ccggggcgcc gctgtgcttg ctcgggccgc tgcgcgagcg cgccgtgccg ggaccggcgc
    10021 gggcgttcgc cgacggctgc agcgactgcg cgtcgcgggc gaactgcgcg ggcgtcgatc
    10081 ccgagtacct gcgccgcttc gggccgggcg agctggcgcc ggtgcggggc ggggaggccg
    10141 aggtcggcgc ggcgcagaga cttcaggaca tgttctcggg gccatgtctc gaagtttcgg
    10201 agggcgcgtg ctggccgatc tgacgccgcc cgcgccgggg tcggagacgg cgcgcggcct
    10261 gttctcgggg ccatggctcg aagttttcgg agggcgcgtg ctggccgatc caacgctgcc
    10321 cgcgccgggg tcggagacgg tgcgcggcct gttctcgggg ccatggctcg aagtttcgga
    10381 gggcgcgtgc tggccgatct gacgctgccc gcgccggggt cggagacggc gcgcgggctg
    10441 ttctcggcgg ccctgcggcg cgcgctgacg gagctgctgc ggctgccggc ggggccgggg
    10501 ccgaacggcg cggcgtgggg gcggttcgcg ccggagctgc ggcggatcgc cgcccgcgcc
    10561 ccgggagccc tggcgagcgc ggtgcggcgg ccgaccgtgg gcaccctgat ccgctgcctg
    10621 cgcgggcgcc ccgacccggc gctgcagggc gagctgctgg cgacgctggc gctcgagctg
    10681 gcggtcgcgg gtgtcctcga ggacaggtgc caggtgccgc agccgccgcc cgaggtgctg
    10741 tgcctgggag cgcgctgcgc cctgcggccg ccgccgggcc cgttgacgat cgcgccgtcc
    10801 gggtgggccg cgggcggcgt cggcgggccg atcgggcagg acttcgggac atgtccgtcc
    10861 gagcatgttt ttaaagatat gt
//
