LOCUS       NZ_CP114040            10831 bp    DNA     circular CON 26-AUG-2024
DEFINITION  Nannocystis poenicansa strain FL3 chromosome, complete genome.
ACCESSION   NZ_CP114040
VERSION     NZ_CP114040.1
KEYWORDS    RefSeq.
SOURCE      Nannocystis poenicansa
  ORGANISM  Nannocystis poenicansa
            Bacteria; Myxococcota; Polyangia; Nannocystales; Nannocystaceae;
            Nannocystis.
REFERENCE   1  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Minimal conservation of predation-associated metabolite biosynthetic
            gene clusters underscores biosynthetic potential of Myxococcota
            including descriptions for ten novel species: Archangium lansinium
            sp. nov., Myxococcus landrumus sp. nov., Nannocystis bai
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13228663)
  AUTHORS   Ahearne,A., Stevens,C. and Dowd,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2022) Biomolecular Sciences, University of
            Mississippi, Faser Hall 407, Oxford, MS 38655, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: IPA v. 10.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: PacBio Sequel IIe
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026965555.1-RS_2024_08_26
            Annotation Date                   :: 08/26/2024 12:45:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,229
            CDSs (total)                      :: 10,129
            Genes (coding)                    :: 10,068
            CDSs (with protein)               :: 10,068
            Genes (RNA)                       :: 100
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 61
            CDSs (without protein)            :: 61
            Pseudo Genes (ambiguous residues) :: 0 of 61
            Pseudo Genes (frameshifted)       :: 9 of 61
            Pseudo Genes (incomplete)         :: 52 of 61
            Pseudo Genes (internal stop)      :: 2 of 61
            Pseudo Genes (multiple problems)  :: 2 of 61
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:28:22
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1180234
            Orig. end                         :: 1191065
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10831
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10831
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10831
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5831](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5831
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            52..1065
                     /locus_tag="O0S08_RS04545"
                     /old_locus_tag="O0S08_04545"
     CDS             52..1065
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012376.5"
                     /locus_tag="O0S08_RS04545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_04545"
                     /product="metallophosphoesterase"
                     /protein_id="WP_269037740.1"
                     /transl_table=11
                     /translation="MQSTLIIGDIHGCHAELLDLLDRAGIGDDDLVVSVGDLVDRGPDP
                     TAVVELFRRRPRSLALCGNHERKHIRGVLSYSQQVARLQLGADYEDHVRWMATLPYHWE
                     RDDVRVVHWGLYPGVPLDEVPEDVRAGTTSGDARLRERFGDRPWYEFYTDDKPVVFGHS
                     VVGPEPLVLRDRIFGLDTGVCHGMRLTGLLLPQFKLVSVPARADHWEQVRREWQAPVLR
                     TLPWQTMTFEQIEKKIRSLRDPELDPSVLDRVQEWVTTLRGALPQLRECLDAEVARISE
                     TAGEEFGRAAAAHPAGSWLLRRRAGKLSPEHLGCSSPQQLLALASAFGVTLSADPV"
     gene            complement(1144..1923)
                     /locus_tag="O0S08_RS04550"
                     /old_locus_tag="O0S08_04550"
     CDS             complement(1144..1923)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS04550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_04550"
                     /product="hypothetical protein"
                     /protein_id="WP_269037741.1"
                     /transl_table=11
                     /translation="MTSGATTTTTSGASSTSSEGGSSVEPTSSAPVDLPGAPCDIWAQD
                     CPPGLKCAPFTVGDFLDETHCVPVDPDPRQLNEPCQTSGTMKEPLDNCDAGLVCGYTDE
                     SGAGHCIPRCSGSPSSPECPEPGETCLTLNQSGTVNVCLAGCSPLDPVCPEGAACAMIG
                     GVDDRFFCVDATDPVRSEYGADCLFEHQCGPGLLCVAKAHVPGCAGERCCTQFCDLAEM
                     EGCPAAPEQVCSPWPGPYRGWEPPPGLENVGFCSAAT"
     misc_feature    complement(2012..2025)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.69"
                     /tool="antismash"
     gene            complement(2097..3248)
                     /locus_tag="O0S08_RS04555"
                     /old_locus_tag="O0S08_04555"
     CDS             complement(2097..3248)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS04555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_04555"
                     /product="hypothetical protein"
                     /protein_id="WP_269037742.1"
                     /transl_table=11
                     /translation="MRSTSLALLAVLLTPPGLARAASRCELTGPPALLGRVAGSDALAG
                     ELDVRSGVAVDVHLVAPGRLDGKPVLFADTGAARHVSWSASGCPKVQVHWRRVEPRMQH
                     VATPPPNAAIAIYANAVVFGPSHGTWLGYDRLEYFETAVPAGDDLWTLTVRDARPSEAA
                     LADARLPEYRDLGTMRLAATVTGPDAVARATPDADDVPRGQIADDVFRYSVRSGDGLFG
                     WLTSYFNVPYVFGSAGVGAKSQAERRVGADCADLIVAGLRRAGRRDLAYSSVGGLVREL
                     GRVTAPLEVRARGDALDPAAYVPRPGDILALDYLDVAALPRPWDHIVVLAVDRGPGGGP
                     ADGRLGPDDLVADIGDAQGLKFAPLGDQGDVRVLVLRARGVEG"
     gene            3350..3760
                     /locus_tag="O0S08_RS04560"
                     /old_locus_tag="O0S08_04560"
     CDS             3350..3760
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF046815.1"
                     /locus_tag="O0S08_RS04560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_04560"
                     /product="DUF488 domain-containing protein"
                     /protein_id="WP_269037743.1"
                     /transl_table=11
                     /translation="MTAAVDLRKGIGDIHTRRWNDPARRGDGTRILVCRYRPRGVRHED
                     ETWAEWLKDVSPSPELLAAFHGKDQEPISDRMFRRRYLNEMRKEPARAQVQALAERVAA
                     GETVTLLCSSGCVDERRCHRTWLRELLLEETG"
     gene            complement(3777..3926)
                     /locus_tag="O0S08_RS04565"
                     /old_locus_tag="O0S08_04565"
     CDS             complement(3777..3926)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS04565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_04565"
                     /product="hypothetical protein"
                     /protein_id="WP_269037744.1"
                     /transl_table=11
                     /translation="MSLQPLPLRHVHEDILHPPAASPARITTGAVVAIVRDSSSPRARA
                     AAAT"
     misc_feature    complement(3909..3911)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3918..3920)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3950..4654)
                     /locus_tag="O0S08_RS04570"
                     /old_locus_tag="O0S08_04570"
     CDS             complement(3950..4654)
                     /EC_number="3.1.3.16"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS04570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_04570"
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_269037745.1"
                     /transl_table=11
                     /translation="MSAFVVSLREPRFGNPVAAEVLGDITVAAVASAWGSWSGLEAEVP
                     AVVGARTSLVADARAGETDWRAALLRAFAVAAVHIDRVPQQEEDDEWTPLTSMTCAVVT
                     PERIHIGWLGGVTACVLDDRRLVRETEPHTLIRQLLAEGNKTAALAGMAHSLGHIIVRS
                     VGPASRGAPSPEFAEWPPLRPGERLLLAPRPTVAALRGHLPLAPAGGDPWLQAAVSTCD
                     DAGHRIGVLVEP"
     misc_feature    4656..4669
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.42"
                     /tool="antismash"
     gene            5001..5831
                     /locus_tag="O0S08_RS04575"
                     /old_locus_tag="O0S08_04575"
     CDS             5001..5831
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629235.1"
                     /locus_tag="O0S08_RS04575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_04575"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_269037746.1"
                     /sec_met_domain="DUF692 (E-value: 9.3e-80, bitscore: 266.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAKIAADGVGVLLLHEYAEALLEADPALDFIEVMPENWARFGGR
                     RRRLFDACRERWPAVAHSISLSVGGPEPIDGEFLAMIAALSPTLGWWSDHLCFGRIGGG
                     YTHALLPLPHSEEAVAHVVRRVKEVQARLGRELVLENVATYARLPVDALAEPAFVRAVV
                     EEADCGLLLDVGNVVVNAHNHGLDPEAYVDALPLTRVREIHVAGHRPRGPLLLDDHTGP
                     VPESVWALYRRTIRAAGRAIPTIVEWETEIPPVEALLAEVARARSESALALEGR"
     misc_feature    complement(5044..5057)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.26"
                     /tool="antismash"
     gene            5831..6523
                     /locus_tag="O0S08_RS04580"
                     /old_locus_tag="O0S08_04580"
     CDS             5831..6523
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS04580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_04580"
                     /product="hypothetical protein"
                     /protein_id="WP_269037747.1"
                     /transl_table=11
                     /translation="MRLSALFSALRPLIDGTADARSVAESLGEVVSPRLGLGLQVYAEA
                     CHKLRRDTVDVIHAGTRAAVTARLGEERWARLVDAYFRERPARCFELNENAAGFGAFAA
                     AQVGAPGWLGELAALEWATWRAESGLDDLRDSSEGPLRAAASVVVLRGAWDVASWLAEE
                     QWAAEPEAGECAVVVWRDRELDACRATPTEEEMAAIEAALAGAPGPWGADEVLTELHAA
                     GILVGAWG"
     gene            complement(6773..7180)
                     /locus_tag="O0S08_RS04585"
                     /old_locus_tag="O0S08_04585"
     CDS             complement(6773..7180)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS04585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_04585"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_269037748.1"
                     /transl_table=11
                     /translation="MSLPRAVLALALVSACARTGPSSRTLHYIDKTLVSSSPVDHRAYA
                     AYIQARVALDAEPADLARALQQIDLALRYDRDDPHLWTTRGEIELRLGDVDAARRSSAR
                     ALAFGPEYPPALQLAAQLQLPGGRQVAEARR"
     misc_feature    complement(7406..7419)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.1"
                     /tool="antismash"
     misc_feature    complement(7629..7647)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.79"
                     /tool="antismash"
     gene            complement(7716..9341)
                     /locus_tag="O0S08_RS04590"
                     /old_locus_tag="O0S08_04590"
     CDS             complement(7716..9341)
                     /EC_number="6.-.-.-"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565406.1"
                     /locus_tag="O0S08_RS04590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O0S08_04590"
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="WP_269037750.1"
                     /transl_table=11
                     /translation="MSDAPKRPSTPDPRAALEQRERLADSYAGPNLLAGGDKDLAVAEK
                     GAAVAVDRLHKSGRQTAHERIELLLDPGSFVELDRFVTTQCPDFDMPGKKVYGDGVVTG
                     HGTVEGRPVFVFAQQAPVFGGSLGWAHAMKICKVMDMATTVGAPVIGLNDSGGARIQEG
                     VASLAGYAEIFKRNVLASGVVPQLSLILGPCAGGAVYSPALTDLVLMVEQSSYMFITGP
                     DVIKTVTHEEVTKEALGGAATHNEKSGVAHFACPSEAAAAAMTRELLGYLPSNNMEDPP
                     HRPTADDPNRSTPELADFVPVDASRPYDIRTIIRAVADDGAFLEVQEHFARNMVIGFCR
                     IDGRSVGVVANQPLVLAGCLDINASVKAARFVRMCDAFNIPLWTLVDVPGFLPGTDQEF
                     NGIIRHGAKLLYAFVEATVPKVTLVTRKAYGGAYDVMASKHIRADVNLAYPTAEIAVMG
                     AEGAVNIIYRRELVEAKNPAAQRTELLNNYRDLFANPYKAAELGFIDQVIRPEDTRRHV
                     SRAFALLKNKRQENPRRKHGNIPL"
     gene            9405..9935
                     /locus_tag="O0S08_RS04595"
                     /old_locus_tag="O0S08_04595"
     CDS             9405..9935
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="O0S08_RS04595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="O0S08_04595"
                     /product="hypothetical protein"
                     /protein_id="WP_269037751.1"
                     /transl_table=11
                     /translation="MREERKSTPVASALWLAACLAACGGGSGSEAAPSKPASPKTEYRC
                     SIQVSKTASEFTGKGNGEDPAKADEAAWTDVCAKLPEAERATCRDETKWTVTKSSMSAS
                     GGGPTSHSTTLALVAIAPMFEGEAFSEASSDEACKAALADACTKAGSPGDCLAAGFEKK
                     GEARTKKTQMSKN"
     misc_feature    complement(10031..10046)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.56"
                     /tool="antismash"
ORIGIN
        1 gcgcgggggc ggcggcgagc gcatcggcga ccgcccggag gagaacgtgt catgcaatcc
       61 acgctcatca tcggcgacat ccacggctgt cacgccgaat tgctcgacct gctcgatcgc
      121 gcaggcatcg gcgacgacga tctggtggtc agcgtcggcg acctcgtcga ccgcgggccc
      181 gacccgacgg ccgtcgtcga gctgttccgc cggcgcccgc gctcgctggc gctgtgcggc
      241 aaccacgagc gcaagcacat ccgcggggtg ctgtcgtatt cacagcaggt cgccaggctg
      301 cagctcgggg cggattacga ggaccacgtg cgctggatgg cgacgctgcc ctaccactgg
      361 gagcgcgacg acgtgcgggt ggtccactgg gggctctacc ccggagtgcc gctcgacgag
      421 gtgccggagg acgtgcgcgc gggcacgacg tcgggcgacg cgcggctgcg cgagcgcttc
      481 ggcgaccggc cctggtacga gttttacacc gacgacaagc cggtcgtgtt cgggcactcg
      541 gtggtggggc ccgagccgct ggtgctgcgc gaccggatct tcggcctcga caccggggtg
      601 tgccacggga tgcggctgac gggcctgctg ctgccgcagt tcaagctcgt gtcggtgccg
      661 gcgcgggccg atcactggga gcaggtgcgc cgcgagtggc aggcgccggt gctgcgcacg
      721 ctgccgtggc agacgatgac gttcgagcag atcgagaaga agatccgctc gctccgcgac
      781 cccgagctcg acccgtcggt gctggaccgc gtgcaggagt gggtgacgac gctccgcggg
      841 gcgctgccgc aattgcgcga gtgcctggac gccgaggtgg cgcggatctc cgagaccgcc
      901 ggcgaggagt tcgggcgggc cgctgcggcg catcccgccg gctcgtggct gctgcgccgt
      961 cgcgccggca agctgtcgcc ggagcacctc ggctgctcgt cgccccagca gctgctggcg
     1021 ctcgcctcgg cgttcggggt gacgctgtcg gccgatccgg tgtgatggcg ccgcgcgccc
     1081 ggggagctcc gggcgcgcgg cgcttcgccg tgtccgatcg cagcgggcgg cccgggcgac
     1141 gcgctacgtc gcggcgctgc agaagcccac gttctccagc cctgggggcg gctcccagcc
     1201 gcgatagggt ccgggccacg gtgaacaaac ctgctcgggc gccgccgggc aaccttccat
     1261 ctcggcgaga tcgcagaatt gcgtgcagca gcgttcgccc gcgcagccgg ggacatgcgc
     1321 tttcgccacg cagagcaggc ccgggccgca ctgatgctcg aacagacagt ccgcgccgta
     1381 ctccgatcgc accggatcgg tcgcatcgac gcagaagaac cggtcgtcga cgcctccaat
     1441 catggcacac gccgccccct cggggcagac agggtcgagc gggctgcagc ccgccaggca
     1501 cacgttgacg gtccccgact ggttcaacgt caaacaggtc tcgcccggct ccggacactc
     1561 gggactggaa gggctccccg agcagcgcgg aatgcagtgc ccggcgccgc tctcgtcggt
     1621 gtacccgcag acgagccccg cgtcgcaatt gtccagcggc tccttcatcg tccctgacgt
     1681 ctggcagggc tcgttcagct gccgagggtc ggggtcgaca ggcacgcaat gggtctcgtc
     1741 gaggaagtct ccgactgtaa acggcgcgca tttcagcccc ggcgggcaat cctgggccca
     1801 gatgtcacag ggggcgccgg gcaggtcgac gggcgcgctc gaagtcggct cgaccgacga
     1861 gccgccctcg gagctcgtgc tggacgcgcc gctcgtcgtg gtcgtcgtgg cgccggaggt
     1921 cacttccgga gatggaacgg ccgcggacgt gtccgagctc gagctgtcca gtcgagcggg
     1981 ggcgcaggcg gccacgatga tgatgcagcg cagaaccttc attcggacgc tcgaagcgga
     2041 tgcggaatcg acggagggtc cttgcaagaa atgtcgtgag gacatgtgcg aacgtgctag
     2101 ccctcgacgc cgcgggcgcg caggacgagg acgcggacgt cgccctggtc gccgagcggg
     2161 gcgaacttga ggccctgggc gtcgccgatg tcggcgacga ggtcgtcggg gccgaggcgg
     2221 ccgtcggccg ggccgccgcc ggggccgcgg tcgacggcga ggacgacgat gtggtcccag
     2281 gggcgcggca aggcggcgac gtcgaggtag tcgagggcga ggatgtcgcc ggggcggggg
     2341 acgtaggcgg cgggatcgag ggcgtcgccg cgggcgcgga cctcgagcgg ggcggtgacg
     2401 cgccccagct cgcggaccag gccgccgacg ctcgagtagg cgaggtcgcg gcggccggcg
     2461 cggcgcaggc cggcgacgat gaggtcggca cagtcggcgc cgacgcggcg ctcggcctgg
     2521 ctcttcgcgc cgaccccggc gctgccgaac acgtacggga cgttgaagta ggaggtgagc
     2581 cagccgaaca ggccgtcgcc gctgcgcacg ctgtagcgga acacgtcgtc ggcgatctgc
     2641 ccgcgcggga cgtcgtcggc gtcgggggtc gcgcgggcga ccgcgtcggg gccggtgacc
     2701 gtggcggcga ggcgcatggt gccgaggtcg cggtactccg gcaggcgggc gtcggcgagg
     2761 gcggcctcgc tgggcctggc gtcgcgcacc gtgagcgtcc acaggtcgtc accggccggc
     2821 acggcggtct cgaagtactc gaggcgatcg tagcccagcc aggtgccgtg cgaggggccg
     2881 aacacgacgg cgttggcgta gatggcgatg gcggcgttgg gcggcggggt ggcgacgtgc
     2941 tgcatgcgcg gctcgacgcg gcgccagtgt acctgtactt tgggacagcc cgaggccgac
     3001 cacgagacgt ggcgggcggc gccggtgtcg gcgaacagca cgggcttgcc gtcgaggcgg
     3061 ccgggcgcga cgagatggac gtcgacggcg acgccgctgc ggacgtcgag ctcgccggcg
     3121 agggcgtcgc tgccggcgac gcggccgagg agcgcgggcg ggcccgtcag ttcgcagcgg
     3181 gaggcggcgc gcgcgaggcc gggcggggtg aggaggactg cgagcagcgc gagcgaggtc
     3241 gaccgcatgg gggcagctga tcgtgacggg ccggcgggcg gtcggcaagc tggacagtgc
     3301 gtgggttttg acgggcgctt cagcgcgcgc gtgccatggt tggggggcga tgactgccgc
     3361 tgtcgacctg cgcaagggga tcggggacat ccatacccgg cggtggaacg atcccgcgcg
     3421 acgcggcgac gggacgcgga tcctcgtgtg ccgctaccgg ccgcgcgggg tccggcacga
     3481 agacgagacg tgggccgagt ggctcaagga cgtgagcccg agcccggagc tgctggcggc
     3541 gttccacggc aaggaccagg agccgatcag cgatcgcatg ttccgccgcc ggtacctgaa
     3601 cgagatgcgc aaagaaccgg cgcgcgcgca ggtgcaggcg ctggccgagc gcgtggccgc
     3661 gggcgagacg gtgacgctgc tgtgctcgtc aggctgcgtc gacgagcgcc gctgccaccg
     3721 cacgtggctg cgggagctgc tgctcgaaga gacaggttga ggcggcggga gcggtgtcag
     3781 gtggcggcgg ccgctcgcgc tcgcgggctc gagctgtcgc gcacgatcgc caccacggcg
     3841 ccggtggtga tcctcgcggg cgaggcggcc ggagggtgga ggatgtcttc atggacatgt
     3901 ctaagaggta atggctgtaa ggacatgttc tttatgcaat acgaggggat cagggctcga
     3961 cgaggacgcc gatccggtgg ccggcgtcgt cgcaggtcga gactgcggcc tgcagccacg
     4021 ggtcgccgcc ggcgggcgcg agcggcaggt ggccgcgcag ggcggcgacg gtgggccgcg
     4081 gggccagcag gagccgctcg ccggggcgca gcgggggcca ctcggcgaac tcgggggacg
     4141 gcgcgccgcg gctggcgggg ccgacgctgc ggacgatgat gtggccgagg ctgtgggcca
     4201 tcccggccag cgcggcggtc ttgttgccct cggcgagcag ctggcggatc agggtgtgcg
     4261 gctcggtctc gcggacgagc cggcggtcgt cgagcacgca ggcggtgacg ccgccgagcc
     4321 aaccgatgtg gatgcgctca ggagtcacga ctgcgcacgt catcgaggtg agcggggtcc
     4381 actcgtcgtc ctcctcctgc tgcggcacgc gatcgatgtg gaccgcggcg acggcgaatg
     4441 ctcgcagcag cgcggcgcgc cagtcggtct cgcccgcgcg cgcgtccgcg acgagggagg
     4501 tccgcgcgcc gacgactgcg ggcacctccg cttcgagccc ggaccacgag ccccacgcgc
     4561 tggcgaccgc ggcgacggtg atgtccccga ggacttcggc ggcgacgggg ttgccgaacc
     4621 ggggctcgcg cagcgagacg acgaaggcgg acatggacaa tgacgataac aggcagcagc
     4681 gcggatgcgg cctgccgtcg agcgcgcgcg cgccttgacc gtgtgatggg cctcagctag
     4741 ctttctcagg cttttactgg aggatatcga tgcagcagaa gaagggtgtc aagagtgggg
     4801 tgaaggtcaa gacgaacgtg cgcgccggct cctgcaaggg ctagctgcgc gatcggggaa
     4861 ttccgggctc cctcgctcgt tctccgccac gccggcggcg ccgcacgctc gcgcgaggga
     4921 ggatcggacc gccgctcacg gacagggcgg cgcgtcgttc gtcggtgccg cgagcggtat
     4981 gatcattggg tgaagaggcg atgaatgcga agatcgcggc cgacggggtc ggcgtgctgc
     5041 ttctgcacga atatgcagag gcgctgctcg aggccgaccc ggcgctcgac ttcatcgagg
     5101 tgatgccgga gaactgggcc cgcttcggcg gacggcggcg gcgactgttc gacgcctgtc
     5161 gcgagcgctg gccggcggtg gcgcacagca tctcgctgtc ggtcggcgga cccgagccga
     5221 tcgatggcga attcctggcg atgatcgcgg cgctctcgcc cacgctgggc tggtggagcg
     5281 atcacctgtg cttcgggcgg atcggcgggg gctacacgca cgcgcttctg ccgttgccgc
     5341 acagcgagga ggcggtggcg catgtggtcc ggcgggtgaa ggaggtgcag gcgcggctgg
     5401 ggcgcgagct ggtgctcgag aacgtggcca cgtatgcgcg cctgccggtc gacgcgctgg
     5461 ccgagccggc gttcgtgcgc gcggtggtcg aggaggccga ttgcgggctg ctgctcgacg
     5521 tcggcaacgt cgtcgtcaac gcgcacaatc acgggctcga cccggaggcc tacgtcgacg
     5581 cgctgccgct gacgcgggtg cgggagatcc acgtggccgg tcatcgaccg cgggggccgc
     5641 tgctgctcga cgatcacacg gggccggtgc cggagagcgt gtgggcgctg tatcgccgga
     5701 cgatccgcgc ggcggggcgg gcgatcccga cgatcgtgga gtgggagacg gagatcccgc
     5761 cggtcgaggc gctgctggcc gaggtggcgc gggcgcggag cgagtcggcg ttggcgctgg
     5821 aggggcgctg atgcggctgt cggcgctgtt ctcggcgttg cggccgctga tcgacgggac
     5881 ggcggacgcg cgttcggtgg cagagtcgct gggcgaggtg gtttcgccgc ggctggggct
     5941 ggggctgcag gtgtacgcgg aggcgtgtca caagctgcgg cgcgacacgg tcgatgtcat
     6001 ccacgcgggc acgcgggcgg ccgtgacggc gcggctgggc gaggagcggt gggcgcggct
     6061 ggtcgacgcg tacttccgcg agcggccggc gaggtgcttc gagctcaacg agaacgcggc
     6121 cgggttcggc gccttcgcgg cggcgcaggt cggcgcgccg gggtggctcg gcgagctggc
     6181 ggcgctcgag tgggcgacgt ggcgggccga gagcgggctg gatgaccttc gggacagctc
     6241 ggaagggccg ctgcgggcgg ccgcgtcggt ggtggtgctg cgcggggcgt gggacgtggc
     6301 gagctggctg gccgaggagc agtgggcggc cgagccggag gcgggcgagt gcgcggtggt
     6361 ggtgtggcgc gaccgcgagc tggacgcttg tcgggcgacg ccgaccgagg aggagatggc
     6421 ggcgatcgag gcggcgctgg ccggggcgcc agggccgtgg ggcgcggacg aggtgctgac
     6481 cgagctgcac gcggcgggga ttctcgtcgg cgcgtggggg tagagcgccc agagcggcgg
     6541 ggcggcgagg acgacgggcc tcgaccggcc gcggatggcg aggcggaggg atgcgtctct
     6601 ggctcgccgc gagcgggccg gcgtcggcga gcgaaacgga tgtggggacg tgggctccgt
     6661 ttcgcctcgc tcgtcgaggc gagagtgagc gaaaggacat gtcctattcg aaatgtcgat
     6721 tagaacatgt ggatgaagac atgtcctgac gggcaggtaa atcctgcggg gctcagcggc
     6781 gggcttcggc gacctggcga ccgccgggga gctggagctg ggcggcgagc tggagcgcgg
     6841 gcgggtactc ggggccgaac gcgagggcgc gggcgctgct gcggcgcgcc gcgtcgacgt
     6901 cgccgaggcg gagctcgatc tccccgcggg tcgtccagag gtgcgggtcg tcgcggtcgt
     6961 agcggagcgc gaggtcgatc tgctgcagcg cgcgggccag gtcggccggc tcggcgtcga
     7021 gggcgaccct cgcctggatg taggccgcgt aggcgcggtg gtcgaccggc gagctgctga
     7081 cgagcgtctt gtcgatgtag tgcagggtcc gcgagctcgg gcccgtccgc gcgcacgcgc
     7141 tgacgagcgc gagggcgagg acggctcggg gcagggacat gcggagagct tgacacattt
     7201 cgcgccgacg cgggttcacg ccggtgtttc cgggccgtgc ggggccgcag gtgggatgag
     7261 cccggcgcgc cggcggccgc gctcgtcacg aggtgggcgg cttcgtgggc acaagccgtg
     7321 aggatggggg gcccggcgtc gcgcgacgcg cgacgcgacg caggtgagcc agcgcgcgct
     7381 cgcccgcgtg ctgacgctca gggggcgagc aggcgtttgc gcggcggttc gcccgggcgg
     7441 tcgcggcggg ggcgacgacc tgtcctttgg gacagatgtc tccggcgggc ggcgaccacg
     7501 ctcgaccggc gaaggtcaag cctgtccttc gggacaggga gttgccggcg agtcgcgggc
     7561 gggggcgagg cgcggcgggc gggggtcaaa cctgtcctcc ggggcaggta gcctccgacc
     7621 cggttcagcc tgtcctttga gacaggacac ttttggcgag gcgcatgcgg cggccgaaga
     7681 acctgtcctt cagggcaggt ggcctccggc ccggatcaca gcgggatgtt gccgtgcttg
     7741 cggcgcgggt tctcctggcg cttgttcttg agcagggcga aggcgcggga gacgtggcgg
     7801 cgggtgtcct cggggcggat cacctggtcg atgaagccga gctcggcggc cttgtagggg
     7861 ttggcgaaca ggtcgcggta gttgttcagg agctcggtgc gctgggcggc ggggttcttg
     7921 gcctcgacga gctcgcggcg gtagatgatg ttgacggctc cctcggcgcc catcacggcg
     7981 atctcggcgg tcgggtaggc gaggttcacg tcggcgcgga tgtgcttgct ggccatgacg
     8041 tcgtaggcgc cgccgtaggc cttgcgggtg acgagggtga ccttcgggac ggtcgcctcg
     8101 acgaacgcgt acagcagctt ggcgccgtgg cggatgatcc cgttgaactc ttgatcggtg
     8161 ccggggagga agccgggcac gtcgacgagg gtccacagcg ggatgttgaa ggcgtcgcac
     8221 atgcggacga agcgggcggc cttgaccgag gcgttgatgt cgaggcagcc ggcgaggacc
     8281 agcggctggt tggcgacgac gccgacgctg cggccgtcga tgcggcagaa gccgatgacc
     8341 atgttgcggg cgaagtgctc ctgcacctcg aggaaggcgc cgtcgtcggc gacggcgcgg
     8401 atgatggtgc ggatgtcgta ggggcgcgag gcgtcgacgg ggacgaagtc ggccagctcg
     8461 ggggtggagc ggttcgggtc gtcggcggtc gggcggtgcg gcgggtcctc catgttgttg
     8521 gagggcaggt agccgagcag ctcgcgggtc atggcggcgg cggcggcctc gctggggcag
     8581 gcgaagtggg ccacgccgct cttctcgttg tgcgtcgcgg cgccgccgag cgcctccttc
     8641 gtgacctctt cgtgggtgac tgtcttgatg acgtcgggcc cggtgatgaa catgtacgag
     8701 ctctgctcga ccatgaggac gaggtcggtg agcgcgggcg agtagacggc cccgccggcg
     8761 cacgggccga ggatcagcga cagctgcggg accacgcccg acgccagcac gttgcgcttg
     8821 aagatctcgg cgtagccggc cagcgaggcc acgccctcct ggatccgcgc gccgccgctg
     8881 tcgttgaggc cgatgaccgg cgcgccgacg gtggtggcca tgtccatgac cttgcagatc
     8941 ttcatggcgt gggcccagcc gaggctgccg ccgaagacgg gcgcctgctg ggcgaacacg
     9001 aacaccgggc ggccttcgac ggtgccgtgg ccggtgacca cgccgtcgcc gtagaccttc
     9061 ttgcccggca tgtcgaagtc ggggcactgg gtggtgacga agcggtcgag ctcgacgaag
     9121 ctgccggggt cgaggagcag ctcgatgcgc tcgtgggcgg tctgccggcc gctcttgtgc
     9181 aggcggtcga cggccacggc ggcgcccttc tcggccaccg ccaggtcctt gtcgccgccg
     9241 gccaggaggt tcggaccggc gtagctgtcg gcgagccgct cgcgttgttc gagggctgcg
     9301 cgcgggtcgg gggtgctcgg ccgcttgggt gcgtcggaca tggcggggcg gaaaatagca
     9361 ggaatcgcgg cgggctcgcg cgcagatgtg gtatggcgcg cggcatgcga gaagagcgca
     9421 agagcacccc ggtggcgtcc gcgttgtggc tggcggcctg cctcgcggct tgcggcggcg
     9481 gcagcggcag cgaggcggcc ccgagcaagc cggccagccc gaagaccgag taccgctgca
     9541 gcattcaggt gagcaagacg gcgagcgagt tcaccggcaa gggcaacggc gaggacccgg
     9601 ccaaggccga cgaggcggcg tggacggacg tgtgcgcgaa gctgccggag gccgagcgcg
     9661 cgacctgccg cgacgagacg aagtggacgg tgacgaagtc gtcgatgtcg gccagcggcg
     9721 gcgggccgac ctcgcactcg acgacgctgg ctctggtggc gatcgcgccg atgttcgagg
     9781 gcgaggcgtt ctcggaggcc agcagcgacg aggcctgcaa ggcggccctg gccgacgcct
     9841 gcaccaaggc cggctcgccc ggcgactgcc tggcggcggg cttcgagaag aagggcgaag
     9901 ccaggacgaa gaagacgcag atgagcaaga attaacgggg agcgacccgc ggccggttcg
     9961 gtgtcccggg gctcgtcgcc cggctcgctg cgagatcgcc tcgcggctcg tgcgctgagc
    10021 cctgcgcagt ggttcgctcc cggcatgcgg tcactgccgg cccacggcga gcccgagcgt
    10081 ggcgggctcg tcggttctcc ggccgaacag cacgtagtag cgacccgggg tgaggtcgtg
    10141 agacgtgatc tcgccgggct ggacttcctc gtcgaagctg tccaggcacg agccgcactt
    10201 gacgacgcgg aagccggacc agccctccgg ctgcgaggcg atgaccaggt agttgccggc
    10261 ctcggtgacc tcgaagccgc gggtcgtgaa catcacgtcg tcctgggcgg ggcccaggac
    10321 atcgtcctgg tcgcaggcga cgtcggcggt gagggtgacc acccggccgt cccacggcgc
    10381 gagcggcagg ctgcagtcga ccagggcgat ccggttgctc atctctcggc acgacgggta
    10441 agcctcgaag tccgccatcg cctgctcgat gggctcgtcg aagatgtcct cgaagacagc
    10501 cgagaactcg acgaaagtgg cccgcgagtc ggccgcgcgc aggaacagga ggtagcggtc
    10561 gaggccgtga cggtcgagca agaaccgcgc gaacaggccg gcggtctggt agtccctggc
    10621 gtcgaacttg aagttgtgga ggacctggtc gagcagggtg tgctcggtgc ggtagccggc
    10681 gccgtcggcg aagacctcgg cgaagccctc ggcgaacggg cgcgggccct cgatgccggc
    10741 cgagttcatc acggcgtggg ttatctcgtg gaagtggaag ctgcggatgc tgaaggcgta
    10801 cggctcgtcc ccgtgggcgc agccgtcggc c
//
