LOCUS       NZ_JAPTYM010000005      2432 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Streptomyces noursei strain ATCC 12757
            contig_5_pilon_pilon_pilon_pilon, whole genome shotgun sequence.
ACCESSION   NZ_JAPTYM010000005
VERSION     NZ_JAPTYM010000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Koduru,L. and Wong,F.T.
  TITLE     Cost-effective hybrid long-short read genome sequencing and assembly
            protocol for GC-rich Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Koduru,L. and Wong,F.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2022) Institute of Molecular and Cell Biology,
            A*STAR, 61 Biopolis Dr, Proteos, Singapore 138673, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-AUG-2022
            Assembly Method                   :: Flye v. 2.8; Pilon v. 1.24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026913165.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 08:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,375
            CDSs (total)                      :: 9,278
            Genes (coding)                    :: 8,845
            CDSs (with protein)               :: 8,845
            Genes (RNA)                       :: 97
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 433
            CDSs (without protein)            :: 433
            Pseudo Genes (ambiguous residues) :: 0 of 433
            Pseudo Genes (frameshifted)       :: 168 of 433
            Pseudo Genes (incomplete)         :: 311 of 433
            Pseudo Genes (internal stop)      :: 17 of 433
            Pseudo Genes (multiple problems)  :: 60 of 433
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:08:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2432
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPTYM010000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..2430)
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (10-298). E-value:
                     7.9e-137. Score: 448.5. Matches aSDomain:
                     nrpspksdomains_O1M07_RS44795_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (402-685). E-value: 1.2e-96.
                     Score: 315.5. Matches aSDomain:
                     nrpspksdomains_O1M07_RS44795_PKS_AT.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742164.1"
                     /locus_tag="O1M07_RS44795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_44780"
                     /product="type I polyketide synthase"
                     /protein_id="WP_268934665.1"
                     /sec_met_domain="mod_KS (E-value: 7.9e-170, bitscore:
                     551.3, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.3e-46, bitscore:
                     145.2, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.1e-98, bitscore: 315.5,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLPAIPEGFEGYIGTGNSGSVASGRVAYTFGLEGPAVTVDTACSS
                     SLVALHLAAQALRRGECSMALAGGVTVMSTPTAFVEFSRQRGLSADGRCRSFSADADGT
                     GWAEGVGMLLVERLSDARRHGHRVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRQALD
                     SAGLSPVDVDVVEAHGTGTRLGDPIEAQALLATYGQGRSSDSPLWLGSVKSNIGHTQAA
                     AGVAGVIKMVMAMRHGQLPSTLHVGEPSPHVDWSAGEVRLLTESVAWPETDRPRRAGVS
                     SFGISGTNAHIVLEQAPAVVEAEPSAEQVPATDGQGPSPVSSPVAVVLSGRSLEAVRAQ
                     AGRLRAQVSGGGERLVDVGFSSVVSRSVFEYRAAVVADDWEGLLAGLDAVNPVRTVGGK
                     TAFLFAGQGSQRAGMGQELAVAYPAFADALDAVCAGFDNAVGGLGSSLREVIASGVGLD
                     ETGWAQPALFAVEVALFRLLEEWGVRPDVVAGHSIGEIAAAHAAGVLSLGDACRLVAAR
                     ARLMQALPAGGAMVAVQAGEAEVAPLLDERVSVAAVNGPNSVVLSGEEDAVLALASRWR
                     GSRLRVSHAFHSPLMDPMLGEFRAVTEQLTFAEPAITLVTGTKTDDVTSAEYWVRQVRE
                     PVRFLGIMQSLESSGVATYLELGPDGVLSALGQDCLPDTDVAAFHPTLRKNQPESLSLV
                     TALAALHTRGAAVDWEAFYAGSDAQRIDLPTYPFQRQRYWLSVPDHFGNVASAGLDEVR
                     HPLLGALVEMPGAESDDVVVTGRVSVRHMPWLADHRVFGSITVPGTALVDMALRAGA"
     source          1..2432
                     /culture_collection="ATCC:12757"
                     /db_xref="taxon:1971"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces noursei"
                     /strain="ATCC 12757"
                     /submitter_seqid="contig_5_pilon_pilon_pilon_pilon"
                     /type_material="type strain of Streptomyces albulus"
     gene            complement(<1..>2432)
                     /locus_tag="O1M07_RS44795"
                     /old_locus_tag="O1M07_44780"
     region          1..2432
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..2432
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2432
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:2430](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2430)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS_motif       complement(6..80)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0011"
                     /evalue="1.40E-10"
                     /label="PKSI-DH_m2"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="808"
                     /protein_start="783"
                     /score="33.6"
                     /tool="antismash"
                     /translation="WLADHRVFGSITVPGTALVDMALRA"
     aSDomain        complement(375..1223)
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O1M07_RS44795_PKS_AT.1"
                     /evalue="1.20E-96"
                     /label="O1M07_RS44795_PKS_AT.1"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="685"
                     /protein_start="402"
                     /score="315.5"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFAGQGSQRAGMGQELAVAYPAFADALDAVCAGFDNAVGGLGSSL
                     REVIASGVGLDETGWAQPALFAVEVALFRLLEEWGVRPDVVAGHSIGEIAAAHAAGVLS
                     LGDACRLVAARARLMQALPAGGAMVAVQAGEAEVAPLLDERVSVAAVNGPNSVVLSGEE
                     DAVLALASRWRGSRLRVSHAFHSPLMDPMLGEFRAVTEQLTFAEPAITLVTGTKTDDVT
                     SAEYWVRQVREPVRFLGIMQSLESSGVATYLELGPDGVLSALGQDCLPDTDVAAFHPTL
                     RK"
     aSModule        complement(375..2399)
                     /domains="nrpspksdomains_O1M07_RS44795_PKS_KS.1"
                     /domains="nrpspksdomains_O1M07_RS44795_PKS_AT.1"
                     /incomplete
                     /locus_tags="O1M07_RS44795"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(513..548)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0010"
                     /evalue="2.30E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="639"
                     /protein_start="627"
                     /score="17.6"
                     /tool="antismash"
                     /translation="YWVRQVREPVRF"
     CDS_motif       complement(666..692)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0009"
                     /evalue="1.30E-02"
                     /label="PKSI-AT-M_m6"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="588"
                     /protein_start="579"
                     /score="8.7"
                     /tool="antismash"
                     /translation="LRVSHAFHS"
     CDS_motif       complement(741..779)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0008"
                     /evalue="3.10E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="563"
                     /protein_start="550"
                     /score="13.9"
                     /tool="antismash"
                     /translation="AAVNGPNSVVLSG"
     CDS_motif       complement(864..986)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0007"
                     /evalue="2.70E-22"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="522"
                     /protein_start="481"
                     /score="71.0"
                     /tool="antismash"
                     /translation="GVRPDVVAGHSIGEIAAAHAAGVLSLGDACRLVAARARLMQ"
     CDS_motif       complement(999..1055)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0006"
                     /evalue="4.50E-04"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="477"
                     /protein_start="458"
                     /score="13.4"
                     /tool="antismash"
                     /translation="ETGWAQPALFAVEVALFRL"
     CDS_motif       complement(1176..1226)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0005"
                     /evalue="7.20E-06"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="418"
                     /protein_start="401"
                     /score="18.4"
                     /tool="antismash"
                     /translation="FLFAGQGSQRAGMGQEL"
     CDS_motif       complement(1437..1469)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0004"
                     /evalue="3.90E+00"
                     /label="PKSI-AT-mM_m5"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="331"
                     /protein_start="320"
                     /score="1.5"
                     /tool="antismash"
                     /translation="VSSPVAVVLSG"
     aSDomain        complement(1536..2399)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O1M07_RS44795_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="7.90E-137"
                     /label="O1M07_RS44795_PKS_KS.1"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="298"
                     /protein_start="10"
                     /score="448.5"
                     /tool="antismash"
                     /translation="GYIGTGNSGSVASGRVAYTFGLEGPAVTVDTACSSSLVALHLAAQ
                     ALRRGECSMALAGGVTVMSTPTAFVEFSRQRGLSADGRCRSFSADADGTGWAEGVGMLL
                     VERLSDARRHGHRVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRQALDSAGLSPVDVD
                     VVEAHGTGTRLGDPIEAQALLATYGQGRSSDSPLWLGSVKSNIGHTQAAAGVAGVIKMV
                     MAMRHGQLPSTLHVGEPSPHVDWSAGEVRLLTESVAWPETDRPRRAGVSSFGISGTNAH
                     IVLEQAP"
     CDS_motif       complement(1758..1802)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0003"
                     /evalue="2.50E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="224"
                     /protein_start="209"
                     /score="13.3"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       complement(1863..1907)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0002"
                     /evalue="1.20E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="189"
                     /protein_start="174"
                     /score="14.0"
                     /tool="antismash"
                     /translation="VEAHGTGTRLGDPIE"
     CDS_motif       complement(2283..2330)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS44795_0001"
                     /evalue="3.50E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="O1M07_RS44795"
                     /protein_end="49"
                     /protein_start="33"
                     /score="22.0"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
ORIGIN
        1 aagcgcccgc cctgagcgcc atgtccacga gcgcggtgcc cggcaccgtg atggacccga
       61 acactcgatg gtcggcgagc catggcatgt gccgaaccga tacccggccg gtcactacca
      121 cgtcgtccga ttcggcgccg ggcatctcca ccagggcacc cagtagggga tgcctcacct
      181 cgtcgagccc agccgatgca acgttgccga aatggtcagg cactgacagc cagtagcgct
      241 gtcgttggaa cggatacgtc gggaggtcga tgcgctgtgc gtcggaaccg gcgtagaacg
      301 cctcccagtc cactgccgcg cccctggtgt gcagagcggc cagcgccgtg accagcgaca
      361 gtgactccgg ctggttcttg cgcagtgtcg gatggaaagc ggcgacgtcg gtgtcgggga
      421 ggcagtcctg ccccagtgcc gagagcacac cgtccggacc cagctccagg taggtcgcca
      481 cccccgagct ttccaggctc tgcatgatgc cgaggaaccg gaccggctcc cgcacctgcc
      541 gcacccaata ctccgccgac gtcacatcgt cggtcttggt ccccgtgacg agggtgatcg
      601 caggctcggc aaaggtgagc tgttccgtca ccgcccggaa ctccccgagc atcgggtcca
      661 tcaggggtga atggaaggcg tgggagaccc gcagacggct gccccgccag cgggaagcga
      721 gagccaggac ggcgtcttcc tcgccggaca ggacaacgga gttggggccg ttgacggcgg
      781 cgacggacac ccgctcgtcc agcagcggag ccacctccgc ttcgcccgcc tgaaccgcca
      841 ccatcgcacc acccgcaggc agcgcctgca tcaagcgggc ccgcgcggcg accagccggc
      901 acgcatcacc cagcgacaac actccggccg cgtgtgcggc tgcgatctca ccgatggagt
      961 gcccggccac cacatcgggt cgcacacccc actcctccaa caaccggaac aacgcaacct
     1021 caaccgcaaa caatgcaggc tgagcccaac ccgtctcatc cagccccaca cccgaagcga
     1081 tcacctcacg caacgacgac ccaagacccc caaccgcgtt gtcgaacccc gcacacaccg
     1141 catccagagc atcagcgaat gccggatacg cgacagcaag ctcctgaccc ataccggccc
     1201 gttgagaacc ctgaccagcg aacaggaaag ctgtctttcc tcccactgtc cggaccgggt
     1261 tcaccgcatc caacccggcc agcagtccct cccagtcgtc ggccactacc gctgcccggt
     1321 actcgaacac cgaccgcgac accaccgagg aaaacccgac atccaccaac cgctcaccac
     1381 cgccggacac ctgcgcccgc aaacgcccag cctgcgcccg caccgcctcc aacgaccgcc
     1441 ccgacaacac caccgcaacc ggtgacgaca ccggggacgg gccctgccca tcggtagcag
     1501 gcacttgttc ggccgagggc tcggcttcaa cgacggcggg ggcctgctcg aggacgatgt
     1561 gcgcgttcgt tccgctgatg ccgaacgagg acactcccgc ccggcgcgga cgatccgtct
     1621 ccggccacgc cacggactcg gtcagcaacc gcacctcacc agccgaccaa tccacgtgcg
     1681 gactcggctc acccacatgc aacgtcgacg gcaactggcc gtgccgcatc gccatcacca
     1741 tcttgatgac acccgccaca cccgcagcgg cctgcgtatg accaatgttc gacttcaccg
     1801 accccaacca caacggagag tccgacgaac gcccctgccc atacgtcgcc aacaacgcct
     1861 gagcctcgat cggatcacct agccgcgtcc cggtcccgtg cgcctccacc acatcaacat
     1921 ccacagggga caaccccgca ctgtccagcg cctgacgaat cacccgctgc tgcgaaggac
     1981 cattcggcgc cgtcaaacca ttcgacgccc cgtcctgatt gaccgcggaa ccacgcacca
     2041 ccgccaacac ccgatgccca tgacgccggg catccgacaa acgctccacc aacaacatgc
     2101 caacaccctc ggcccaaccc gtcccatcag catccgccga gaacgacctg caccgaccat
     2161 ccgccgacaa cccacgctga cgcgaaaact ccacaaacgc cgtcggcgtc gacatcaccg
     2221 tcacaccacc ggccaacgcc atcgaacact caccacgacg cagtgcctgc gcagccaaat
     2281 gcagcgcaac caacgacgac gaacacgccg tgtccaccgt caccgccggc ccctccagac
     2341 caaacgtgta agcaacccga cccgaagcga cactgcccga attccccgta ccgatgtatc
     2401 cctcgaatcc ctcaggaata gccggaagac gt
//
