LOCUS       NZ_JAPTYM010000001     10990 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Streptomyces noursei strain ATCC 12757
            contig_1_pilon_pilon_pilon_pilon, whole genome shotgun sequence.
ACCESSION   NZ_JAPTYM010000001
VERSION     NZ_JAPTYM010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10059690)
  AUTHORS   Koduru,L. and Wong,F.T.
  TITLE     Cost-effective hybrid long-short read genome sequencing and assembly
            protocol for GC-rich Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10059690)
  AUTHORS   Koduru,L. and Wong,F.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2022) Institute of Molecular and Cell Biology,
            A*STAR, 61 Biopolis Dr, Proteos, Singapore 138673, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-AUG-2022
            Assembly Method                   :: Flye v. 2.8; Pilon v. 1.24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026913165.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 08:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,375
            CDSs (total)                      :: 9,278
            Genes (coding)                    :: 8,845
            CDSs (with protein)               :: 8,845
            Genes (RNA)                       :: 97
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 433
            CDSs (without protein)            :: 433
            Pseudo Genes (ambiguous residues) :: 0 of 433
            Pseudo Genes (frameshifted)       :: 168 of 433
            Pseudo Genes (incomplete)         :: 311 of 433
            Pseudo Genes (internal stop)      :: 17 of 433
            Pseudo Genes (multiple problems)  :: 60 of 433
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:08:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8795864
            Orig. end                         :: 8806854
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPTYM010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10990
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="38"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10990
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10990
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5990](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5990)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(91..1353)
                     /locus_tag="O1M07_RS38775"
                     /old_locus_tag="O1M07_38765"
     CDS             complement(91..1353)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537760.1"
                     /locus_tag="O1M07_RS38775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38765"
                     /product="cytochrome P450"
                     /protein_id="WP_372516491.1"
                     /sec_met_domain="p450 (E-value: 7.6e-23, bitscore: 80.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNPMESAEFSRNPYPLLAALRAGGPVRRMRTGKDRTTWVVTGWA
                     EARAALADRRLSKDTARYFADRPSGRDLAPAISRSMLATDPPDHGRLRKLAMTAFTPAA
                     VSRLEPRIREIAEGLAAALAGAAQAAGGGPVDLVEGFAAPLPITVICELLAVPDADRAA
                     VRRWSDDLFAAADPDTADRASHALAGYLAELIAARRAAPGDDVLSGLIAARDAGDRLSE
                     PELVSLAALLVVAGHETTANLIGNGLLALLRDDALRARLRDDAGLLPAAVEEFLRFDAP
                     VTLATFRYATEPFDLGGVRIGAGDVVVVSPGGANRDPARFDEPDAVRLDRPAPGGHVSF
                     GHGPHHCLGAPLARAEARIAFAVLLARFPALRPATDGRDASGGPDGVSWRRTRLMRGPA
                     RLPALLGPIAGGTNGAPPARP"
     gene            1525..1752
                     /locus_tag="O1M07_RS38780"
                     /old_locus_tag="O1M07_38770"
     CDS             1525..1752
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092057.1"
                     /locus_tag="O1M07_RS38780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38770"
                     /product="hypothetical protein"
                     /protein_id="WP_039629879.1"
                     /transl_table=11
                     /translation="MSEHPPVIVHPPTPSGGRRVTVHGQIVGLAHGRGDVAEFLRRAGV
                     AGPAEDIALDDPRLVEWRGGSLDDWPMPSP"
     gene            1971..3449
                     /locus_tag="O1M07_RS38785"
                     /old_locus_tag="O1M07_38775"
     CDS             1971..3449
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537758.1"
                     /locus_tag="O1M07_RS38785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38775"
                     /product="DNA-3-methyladenine glycosylase 2 family protein"
                     /protein_id="WP_189865093.1"
                     /transl_table=11
                     /translation="MSEDSRYEAVRSRDARFDGAFFFAVSTTGIYCRPSCPATTPRRRN
                     VTFFPTAAAAQGSGFRACRRCRPDAVPGSVEWNARADVVGRAMRLIGDGVVDREGVAGL
                     ADRLGYSARQVQRQLTAELGAGPAALARAQRAHTARVLLQTTGLPIADVAFAAGFASVR
                     QFNDTIKEIYALTPSGLRAVRPGPGSRFGGLAAPSGTPGVLPLRLAYRGPYAARQIFDY
                     LAQRALGGIEEVTGEPGGRTYRRTLRLPHGTGVAEVDEATGDGWLDCRLHLTELRDLAT
                     AAQRVRRLFDLDADPFAVGERLGADPALAPLVARCPGLRSPGTVDPDELAVRAVLGRRA
                     GTAAGRALGTRLVAAHGEPLPRPAGTLGHLFPRAVELADAPLTGLGLSDARIAALRALA
                     AALSARDVVLDAGTDRDRAERALRGLPGIGPWTADYIRMRALGDPDVLLGAPPAGTDPA
                     VWSPWRSYATHHLWAADPAGIAERHPNPTERIDA"
     gene            3446..3955
                     /locus_tag="O1M07_RS38790"
                     /old_locus_tag="O1M07_38780"
     CDS             3446..3955
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574649.1"
                     /locus_tag="O1M07_RS38790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38780"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_189865068.1"
                     /transl_table=11
                     /translation="MTLYATLDSPLGELLLVGERSATAPGGTALASLSVPGQKRGAVVQ
                     DDWTHDPAAFAGIARQLRAYFDGGQQPFAVECVPRGTDFQQRVWAALEAIPYGTTTTYG
                     AIAAAIGAPRGAVRAIGTAIGANPLLVVRPCHRVIGADGSLTGYAGGLARKQLLLGLEG
                     AAPEAR"
     gene            complement(4019..4783)
                     /locus_tag="O1M07_RS38795"
                     /old_locus_tag="O1M07_38785"
     CDS             complement(4019..4783)
                     /NRPS_PKS="Domain: PKS_KR (7-183). E-value: 1.3e-07. Score:
                     23.7. Matches aSDomain:
                     nrpspksdomains_O1M07_RS38795_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="O1M07_RS38795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38785"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_189865066.1"
                     /transl_table=11
                     /translation="MGDLYGKTALVTGSSRGIGRGIAQRLAQDGALVAVHYGSNEGAAK
                     ETVEGIRDNGGRAFLVGAELGLPGDAEALFDAFDTGLAAAGTEPGLDILVNNAAISLPG
                     HIGEVTTEEFDRTIAVNTKAPFFIIQHGLRRMRDGGRIVNISSAVTSTAFPSTIAYGLS
                     KGAIDTLTRTLAKDVGERGITVNTVAPGFIETDMNAAMRSTPEAHAALSAISVFNRLGR
                     PADVADVVAFLASDDSRWITGQRIDVTGGSML"
     aSDomain        complement(4235..4762)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O1M07_RS38795_PKS_KR.1"
                     /evalue="1.30E-07"
                     /label="O1M07_RS38795_PKS_KR.1"
                     /locus_tag="O1M07_RS38795"
                     /protein_end="183"
                     /protein_start="7"
                     /score="23.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIAQRLAQDGALVAVHYGSNEGAAKETVEGIR
                     DNGGRAFLVGAELGLPGDAEALFDAFDTGLAAAGTEPGLDILVNNAAISLPGHIGEVTT
                     EEFDRTIAVNTKAPFFIIQHGLRRMRDGGRIVNISSAVTSTAFPSTIAYGLSKGAIDTL
                     TRTLAKDVGERGI"
     CDS_motif       complement(4691..4756)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_O1M07_RS38795_0001"
                     /evalue="1.10E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="O1M07_RS38795"
                     /protein_end="31"
                     /protein_start="9"
                     /score="11.7"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIAQRLAQDGA"
     gene            complement(5001..5990)
                     /locus_tag="O1M07_RS38800"
                     /old_locus_tag="O1M07_38790"
     CDS             complement(5001..5990)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="O1M07_RS38800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38790"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_372516430.1"
                     /sec_met_domain="AfsA (E-value: 8.5e-22, bitscore: 76.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTLQASSTVPVTSRRRMQQVQNPSAASRPGSDTSAPIPHEFLH
                     RPLVEDILITSWRRLGQDRFSVTAQWPHDHLYFAPHDSRPHPMLAGETIRQAGLLLCHT
                     EFGVPLGHHFILHDLVYTADPAHRVSGNGPTQLSIDVVCSQTRMRAGMLSSSRFDMTIR
                     KDGRVVATGHSHVSVTSPAVYRRVRGERITARHVPGPLPTPVPPGLTGSAAERDVLLSP
                     AGQPGRWLLRIAPGHPTVVNPANDHVPGLSLLDAAQQAARALTRSSSFLPYAFGTEFHR
                     YAEHGLPCLIEARRKMPTATGTTTVLVTGTQDGERVFASVLTAMDARD"
     misc_feature    complement(6054..6067)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.86"
                     /tool="antismash"
     gene            6165..6863
                     /locus_tag="O1M07_RS38805"
                     /old_locus_tag="O1M07_38795"
     CDS             6165..6863
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032559.1"
                     /locus_tag="O1M07_RS38805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38795"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_189865064.1"
                     /transl_table=11
                     /translation="MARQDRAIKTRRVILETAAAVFDEHGFDRATIAEILSRAGVTKGA
                     LYFHFPSKEDLALAVLHEQVLDIAVLPQPIKLQEFVDAGQILACRLRHDPIQRGAARLA
                     IEPGANQLDRKQSMSAWTTFVEGLLREAKARGEVLESVNVRDTAELFVGAFAGLQMMSK
                     AFTDQNDVSHRLLVFFEHTLPTIAVPAVLAKLRLDPERGERLDAELRERAAEEAEHAAA
                     LEADLQNAAP"
     misc_feature    complement(7137..7152)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            7296..9707
                     /locus_tag="O1M07_RS38810"
                     /old_locus_tag="O1M07_38800"
     CDS             7296..9707
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537753.1"
                     /locus_tag="O1M07_RS38810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38800"
                     /product="MFS transporter"
                     /protein_id="WP_189865062.1"
                     /transl_table=11
                     /translation="MTQTMTDQLPAGAADSAAAPPLPPPAKGGHRGTHAGIVPVLAFAG
                     IVVAVMQTLLVPVIKDLPTLLGTAPSNATWVMTATLLAGAVATPIMGRLGDLYGKRRML
                     LTSLAVMVVGSLICGFTDQLIVMIVGRALQGFAMGAIPLGIGLMRDELPRERLGSAMAL
                     MSSSIGVGGGLALPAAALVAQHADWHTLFFAAAGLGVLSMLLTVLVVPETSVRAPGRFD
                     VAGALGLSVGLVALLLPITKASEWGWGSPRTLGLFGAAALILVLWGVMELRIADPLVDL
                     RTTARREVLLTNLASITVGVAFYAISLVLPQLLQLPTSTGYGLGQSMVVAGLCVAPLGL
                     TMMFVAPLYARIAARRGPKVSLLLGMLVIGVGYAGGIGLMQAPWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLARMSEPLGPVAVPTM
                     AGFRVSFLIATAAVAVGVLVAAFLPSQRKAAGARATPAAQNADETGTAAAGPVAAEGAD
                     AAGPGAAVAADVPADESTTGFRGRVVDTAGGPVPGATVTLIDRHGQQAGVTTAAPDGSY
                     ALAAPAAGTYVLTGAAPAHAPQAVSAAHAGEGTVAGADLVLTPTGSRLAGTVLGGPEGT
                     ALTGARVVITDAHGDVVTHATTGADGRWTVAHLPDGPYTLVFSAPGHQPTAHAVQLGGG
                     GAGADARGDTRHQEVRLRPAATVRGTVRGPDGHPLADAAVTLLQDGTVAGHAVTGPDGA
                     YAFADLAGRHYTLSAAGYPPHAAPISLAGGADARLDLELAQPGGAGQRER"
     gene            complement(9842..10546)
                     /locus_tag="O1M07_RS38820"
                     /old_locus_tag="O1M07_38810"
     CDS             complement(9842..10546)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537752.1"
                     /locus_tag="O1M07_RS38820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_38810"
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="WP_189865060.1"
                     /transl_table=11
                     /translation="MPLEALRPSPLVEQAAQQLRDQITGGHWPVGSRLPGETTLAKTLG
                     VGRSTVREALRALAGAGMVQPRQGAGVFVIAAEPTEDWPTRLRRAAVADVYEVRAMVEV
                     QAARLAAERRTEADVTALYAALERRRADGADDAAFVDADIALHAAVVDAAHNPVLTDLF
                     GQFVPALRQGLIDLVRLLGLRDHDPHHGDGRHAALVEAVAAGDADTAGDVLQSELAETR
                     ALLLTGRTAENE"
     misc_feature    10615..10628
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.78"
                     /tool="antismash"
ORIGIN
        1 accgccgcac tgcggtcggg gcgccggtgc cttgcagcgg gccgtcgcat gccgaacggc
       61 cgggccgggc cgtccgggaa ccgcctggcc tcaaggccgt gccggcggcg ccccgttcgt
      121 gccgcccgcg atcggaccca ggagcgctgg gagccgggcc gggccgcgca tgagccgggt
      181 ccgtcgccaa ctgaccccgt cggggccgcc ggaggcgtcc cggccgtccg tggcgggccg
      241 cagtgcgggg aagcgggcga gcagtaccgc gaacgcgatc cgggcctcgg cccgggccag
      301 cggcgcgccc aggcagtggt gcggtccgtg cccgaaggac acgtggccgc ccggggccgg
      361 gcggtccagc cgtaccgcgt ccggctcgtc gaaccgcgcc gggtcgcggt tggcgccacc
      421 gggcgagacc acgaccacgt cccccgcccc gatgcggacg ccgccgaggt cgaacggctc
      481 ggtggcgtaa cggaaggtcg ccaacgtcac cggcgcgtcg aaccggagga actcctccac
      541 cgcggcgggc agcagccccg cgtcgtcccg caggcgggcg cgcagcgcgt cgtcgcggag
      601 cagggccagc aggccgttgc cgatcagatt ggccgtggtc tcgtgcccgg cgaccaccaa
      661 cagcgcggcc agcgagacga gttcgggctc gctgagccgg tcaccggcgt cccgggcggc
      721 gatcaagccg ctcagcacgt cgtccccggg cgccgcgcgc cgcgccgcga tcaactcggc
      781 caggtacccg gccagggcgt gcgaggcccg gtcggcggtg tccggatccg ccgccgcgaa
      841 caggtcgtcg gaccaccggc gcaccgcagc ccggtccgcg tccggcaccg ccaacagctc
      901 gcagatcacc gtgatcggca gcggcgccgc gaacccctcg accagatcga cggggccgcc
      961 gcccgccgcc tgcgcggccc cggccagcgc cgcggccagc ccctccgcga tctcccggat
     1021 gcggggctcc agccggctca ccgccgcggg ggtgaaggcc gtcatggcca gtttccgcag
     1081 ccggccgtgg tccggcgggt cggtggcgag catgctccgg ctgatggccg gtgccagatc
     1141 gcgtccggag ggccggtccg cgaagtaccg tgcggtgtcc ttcgacaacc gccggtcggc
     1201 cagcgccgcg cgtgcctccg cccagccggt caccacccac gtcgtgcggt ccttgccggt
     1261 ccgcatccgc cgcaccgggc cgcccgcccg gagcgcggcg agcagcgggt aggggttgcg
     1321 ggagaactcg gccgactcca tggggttgtt cactgccacc ctcgtgctct cgttcgcggt
     1381 caggtccgcg cccgccctcc cggaccggcc cggcgaccac cttcgcaccg gcgccggtcg
     1441 ccccgtccgg tccggatccc agccgtccga gtgagatccc accgtcgagg tgcgagcgcg
     1501 cggagcccgg cgcacgctgg aggcatgtcc gagcacccgc ctgtcatcgt gcacccaccg
     1561 acgccctccg gtggacgccg ggtgaccgtg cacgggcaga tcgtcggcct ggcccatggc
     1621 cgcggcgacg tggcggagtt cctccggcgc gcgggtgtcg ccggccccgc cgaggacatc
     1681 gcactggacg acccccgcct ggtcgagtgg cgcggcggct ccctggacga ctggccgatg
     1741 ccgtcgccgt gacgggcccc ggccgggcac ctccgagatg tgcccggccc gtcctccgcc
     1801 gagcccacgc cggccccccg ctcgcatccg tgggtacccg ttgtcgctca acctggtccg
     1861 caggcccgcc gccccggccg ggcccctcgc cccggccgtc gaggcaccga aagcgccgtg
     1921 tccgatttcc gccagagtgg tggcgcggaa tgacgcacag tgggggtgtg atgagcgagg
     1981 acagcaggta cgaggcggtg cggagccggg acgccaggtt cgacggggcg ttcttcttcg
     2041 cggtgtcgac gacggggatc tactgccggc cgagctgccc cgcgaccacc ccgcggcggc
     2101 ggaacgtcac cttcttcccg accgcagcgg ccgcgcaggg ctccggattc cgggcctgcc
     2161 ggcggtgccg cccggacgcg gtccccggct cggtcgagtg gaacgcccgg gcggacgtcg
     2221 tggggcgggc gatgcggctg atcggcgacg gggtggtcga ccgggagggc gtcgccgggc
     2281 tggccgaccg gctcggctac agcgcccggc aggtgcagcg gcagctgacc gcggagctcg
     2341 gcgcagggcc cgccgccctg gcccgggcgc agcgcgcgca caccgcgcgg gtgctgctgc
     2401 agaccaccgg actgccgatc gccgacgtcg ccttcgccgc cggattcgcc agcgtccggc
     2461 agttcaacga cacgatcaag gagatctacg cgctcactcc gagcggactg cgggcggtcc
     2521 gccccgggcc cggttcccgc ttcggcggcc tcgcggcccc gtccggcacg cccggtgtgc
     2581 tgccgctgag gttggcgtac cgcggcccgt acgcggcccg gcagatcttc gactacctgg
     2641 cgcagcgggc gctcggcggg atcgaggagg tcaccgggga gcccggcggc cggacgtacc
     2701 ggcgcaccct gcggctgccc cacggcaccg gtgtcgcgga ggtcgacgag gccaccggcg
     2761 acggctggct ggactgccga ctgcacctca ccgagctgcg ggacctcgcc accgccgccc
     2821 agcgcgtccg ccgcctgttc gacctggacg ccgacccgtt cgcggtcggc gaacgcctcg
     2881 gcgcggatcc cgcgctggcc ccgttggtcg cccgttgccc ggggctgcgc tccccgggga
     2941 ccgtggaccc ggacgaactc gcggtgcgcg ccgtcctcgg ccggcgggcc gggaccgccg
     3001 ccggccgggc cctgggcaca cgcctggtcg ccgcgcacgg ggagccgttg ccacggcctg
     3061 ccggcaccct cggccatctc ttcccgcggg ccgtcgagtt ggcggacgcg ccgctcaccg
     3121 ggctcggcct gtccgacgcg cggatcgccg cgctgcgcgc cctcgccgcc gcactcagcg
     3181 cccgggacgt cgtcctggac gccgggaccg atcgcgaccg ggccgagcgc gcgctccgcg
     3241 gcctccccgg catcggtccg tggaccgccg actacatccg gatgcgggcg ctgggcgacc
     3301 ccgatgtcct cctcggcgca ccgccggcgg gcaccgaccc ggcagtgtgg agcccctggc
     3361 gctcgtacgc gacgcaccac ctctgggccg ccgaccccgc cggcatcgcg gaacggcacc
     3421 cgaacccgac agaacggatc gacgcatgac cctctacgcc accctcgaca gccccctggg
     3481 cgaactcctg ctggtcggcg agcggtccgc caccgcgccc ggcggcaccg cgctcgcctc
     3541 gctctccgtg ccgggccaga agcgcggggc cgtcgtccag gacgactgga cgcacgaccc
     3601 ggccgcgttc gccgggatcg cccgccagct ccgggcgtac ttcgacgggg gacagcagcc
     3661 gttcgccgtc gagtgcgtcc cccgcggcac ggacttccaa cagcgcgtct gggcggcgct
     3721 ggaggccatc ccgtacggca cgaccaccac ctacggggcg atcgcggccg ccatcggggc
     3781 gccgcgcggt gccgtccgcg ccatcgggac ggcgatcggc gccaacccgc tgctggtggt
     3841 ccggccctgc caccgcgtca tcggcgccga cgggtcgctc accggctacg ccggcggcct
     3901 ggcccgcaag caactgctcc tcggcctcga aggggcggcg ccggaggccc gttgagccgg
     3961 ccgaacgcgg ccgcgtccgc cggtccgggc accgccccgg accggcggac cgggtaggtc
     4021 agagcatcga cccaccggtg acgtcgatgc gctggccggt gatccaacgg gagtcgtcgg
     4081 aggcgaggaa ggcgaccacg tccgcgacgt cggcgggtcg gccgagccgg ttgaacaccg
     4141 aaatggcgga aagtgccgca tgtgcctcgg gagttgaccg catggccgcg ttcatgtcgg
     4201 tctctatgaa tccgggcgcc acggtgttca ccgtgatccc gcgctcgccg acgtccttgg
     4261 cgagggtccg ggtgagggtg tcgatcgcgc ccttggagag cccgtaggcg atggtcgagg
     4321 ggaaggccgt actcgtcacc gcggacgaga tgttgacgat ccgtccgccg tcccgcatcc
     4381 gccgcagccc gtgctggatg atgaagaacg gggccttggt gttgaccgcg atggtgcggt
     4441 cgaactcctc ggtcgtcacc tcgccgatgt gccccggcag gctgatcgcc gcgttgttga
     4501 cgaggatgtc caggccgggt tccgtgcccg ccgcggccag cccggtgtcg aaggcgtcga
     4561 acagcgcctc ggcgtcgccc gggagcccca gctccgcgcc gaccaggaac gcccggccgc
     4621 cgttgtcccg gatgccctcg acggtctcct tggccgcgcc ctcgttgctc ccgtaatgga
     4681 cggcgaccag ggctccgtcc tgcgcgagac gctgggcgat gccccgcccg atgccgcggc
     4741 tggatcccgt aaccagcgcg gtctttccgt aaaggtcacc catggtcgtt gtcggccttc
     4801 cgtagtgctt ctggcggtcg gttgccgccg cgtcaaaggg gagtgctgtg cgggcggccc
     4861 ggccgttgtt ccggtatgtc gtctgcctac acggtgcatg agccggtcgg gctcatgacg
     4921 aaggggggtg gcgcgtgcgc gccggcgctc atggggcggt gggctcggac gggcggttcg
     4981 gcggcggccc gcccgtccgg tcagtcgcgc gcatccatgg cggtcaggac ggaggcgaag
     5041 acccgctcgc cgtcctgggt cccggtgacc aggaccgtcg tggtgccggt ggccgtcggc
     5101 atcttccgcc gcgcctcgat cagacacggg aggccgtgtt ccgcgtaacg gtggaactcg
     5161 gtgccgaaag cgtagggcag gaaggagctg cttctggtca gcgcgcgggc ggcctgctgc
     5221 gccgcgtcga gcagggacag tccgggcacg tggtcgttgg cggggttgac caccgtgggg
     5281 tggccgggcg cgatccgcag cagccaccgg cccggctgcc cggcggggga gagcaggacg
     5341 tccctctcgg cggcgctgcc ggtcagcccc ggcgggacgg gggtggggag cgggccgggg
     5401 acgtggcggg ccgtgatccg ttccccgcgg acccggcggt agacggccgg cgaggtgacg
     5461 ctgacatggg agtggccggt cgcgacgacg cggccgtcct tgcggatcgt catgtcgaag
     5521 cggctgctgg acagcatgcc ggcgcgcatc cgtgtctggc tgcacacgac gtcgatgctc
     5581 agctgcgtcg gcccgttccc ggagacgcgg tgcgcggggt cggcggtgta gacgaggtcg
     5641 tgaaggatga agtgatgacc gagcggtacc ccgaactcgg tgtggcagag cagcagtccg
     5701 gcctgtcgga tggtctctcc cgccagcatc ggatggggcc ggctgtcgtg gggagcgaag
     5761 tagaggtgat catgcggcca ctgggcggtc acggagaagc ggtcctggcc cagtcggcgc
     5821 caggaagtga tcaagatgtc ctccacgaga gggcggtgca ggaactcgtg agggatcggg
     5881 gcactggtgt cggaccccgg ccggcttgcg gccgatgggt tctggacctg ctgcatccgg
     5941 cgtcggctcg tgactggaac cgtgctgctg gcctgcaacg tgtcgctcat gacagcccct
     6001 atgtgtgtct ctacggaaga gctctggaca cgattaagat acgtaccatc cggttttctt
     6061 ttcaatgccg atgtgagggt aaaggcgatc cgtcgatttg gaggagactt gagctgtggc
     6121 gaaatactga gaagcttgag cctttgacag acaggaggcg ctctgtggca cgtcaggacc
     6181 gcgcaatcaa gacgcgacga gtgatcttgg aaaccgccgc ggcggtcttc gatgaacacg
     6241 gcttcgaccg cgcgacgatc gctgagattc tctcgcgcgc cggcgtgacg aagggcgccc
     6301 tgtatttcca ctttccctcc aaagaagatc ttgcgctcgc cgtgctgcac gagcaggtgc
     6361 tcgatattgc ggtgctgccg cagccgatca aactccagga gttcgtggac gccgggcaga
     6421 tcctggcgtg ccggctgcga cacgatccga tccagcgcgg cgccgcccgt ctcgccatcg
     6481 agccgggtgc caatcagctc gatcgcaagc agtcgatgtc ggcgtggacc acgttcgtcg
     6541 aggggctgct cagggaggcc aaggcgcggg gcgaagtcct ggagagcgtc aacgtgcggg
     6601 acacggcaga gctgttcgtc ggtgccttcg ccgggcttca gatgatgtcc aaggcgttca
     6661 ccgaccagaa cgacgtcagt catcggctgt tggtcttctt cgagcacacc ctgcccacca
     6721 tcgcggtgcc ggccgttctc gccaaactca ggctcgaccc ggagcggggt gagcggctcg
     6781 atgcggagct ccgggaacgc gcggcggagg aggcggagca cgccgccgcg ctggaggcgg
     6841 acctgcagaa cgcggcgccg tagcgcgccg cggctgcacc tcggggtgcg gatgtcgcgg
     6901 ggcggaaggt ccccgggtgt gctgatacgt cggttccgtc ccgggcgcgg ccccgccgcc
     6961 cgcgctccgc tcttgaccca gtcttgatcc ctcggccggt cgattgtcag tggcgtgcgg
     7021 ttcactggat cccgtgtccg gaacgaggcc gggggagggg cgatggacgg gacggtgggc
     7081 ccgaaggggc gggccgcgta cggaagttga tgcaggcggg ggccgggtgt ggtcggcgtg
     7141 agcgagatca ccgtccggag tccgagatct ggccgggtgg cttggaatag gcccccgagc
     7201 tgaaacgttc gccatgcacg tatcaagggg ccacgcccct gttcgcgctc cgcgcaccct
     7261 ctccgctcgc tcctgccgcc ctggaggccg cacgcatgac ccagacgatg acggaccagc
     7321 tccccgcggg ggctgcggac tccgccgccg cgccgccact gcccccgccc gccaagggcg
     7381 gacaccgcgg aacgcacgcc ggcatcgtcc cggtgctcgc cttcgccggc atcgtcgtcg
     7441 cggtgatgca gacgctgctc gtgccggtca tcaaggacct gccgaccctg ctcggcaccg
     7501 cgcccagcaa cgccacctgg gtcatgacgg ccaccctcct cgccggtgcg gtcgccaccc
     7561 ccatcatggg ccgcctcggc gacctctacg gcaagcggcg gatgctgctg acgagtctcg
     7621 ccgtcatggt cgtgggctcg ctgatctgcg gcttcaccga ccagttgatc gtcatgatcg
     7681 tcgggcgggc gctgcagggc ttcgcgatgg gcgcgatacc cctcggcatc gggctgatgc
     7741 gcgatgaact gccccgggag cggctcggct cggccatggc cctgatgagc tcctcgatcg
     7801 gcgtcggcgg cggactggcg ctgcccgccg cggccctggt ggcccaacac gccgactggc
     7861 acacgctgtt cttcgccgcc gccgggctcg gcgtgctgtc gatgctgctc accgtgctgg
     7921 tcgtcccgga gacctcggtg cgggcgcccg gccgcttcga cgtcgccggc gccctcggcc
     7981 tgtcggtcgg cctggtcgcc ctcctgctgc cgatcaccaa ggcgagcgag tggggttggg
     8041 gatcgccccg caccctcggc ctgttcgggg ccgcggcgct gatcctggtg ctgtggggcg
     8101 tgatggagct gcggatcgcc gacccgctgg tggacctgcg caccaccgcc cggcgcgagg
     8161 tgctgctcac caacctcgcc tccatcacgg tcggggtggc gttctacgcc atctcgctcg
     8221 tgctgccgca gctgctccag ctgcccacgt cgaccggcta cggcctcggc cagtcgatgg
     8281 tggtggccgg gctgtgcgtg gcgccgctgg ggctgacgat gatgttcgtg gcaccgctgt
     8341 acgcacggat cgcggcccgc cgcggcccca aggtctcgct gctgctcggc atgctggtca
     8401 tcggggtcgg ttacgccggc gggatcggcc tgatgcaggc tccctggcag acggtcatca
     8461 tcgcggtggt ggtcggcgcg ggcatcggcc tggcgtactc ctcgctcccg gcgctcatca
     8521 tcggggcagt cgacccgtcc gagaccggcg cggccaacgg cctgaacacc ctgatgcgtt
     8581 cgatcggcac gtccgtgtcc agcgccgtca tcggcatggt gctggcccgt atgtccgagc
     8641 cgctggggcc ggtcgcggtg cccacgatgg ctgggttccg ggtctcgttc ctgatcgcca
     8701 ccgccgcggt cgccgtcggg gtgctcgtgg cggccttcct gccgtcccag cggaaggccg
     8761 ccggggcgcg ggcgacgccg gcggcgcaga acgcggacga gaccggcacg gcggcggccg
     8821 gcccggtcgc ggccgagggg gccgacgcgg ccggtcccgg cgccgcggtc gcggcggacg
     8881 tcccggcgga cgagtcgacc accgggttcc gggggcgggt cgtggacacc gcaggcggcc
     8941 cggtgccggg cgccacggtc accctgatcg accggcacgg gcagcaggcc ggggtcacca
     9001 cggccgcgcc cgacggcagt tatgcgctgg ccgccccggc cgcgggcacc tacgtcctga
     9061 ccggcgccgc cccggcccac gccccgcagg ccgtctccgc cgcgcatgcc ggcgagggca
     9121 cggtggccgg ggcggacctg gtgctgacgc ccaccggcag ccgcctcgcc ggcaccgtgc
     9181 tcggtggccc ggaagggacg gcgctgaccg gagcgcgcgt cgtgatcacc gatgcccacg
     9241 gcgacgtggt gacgcacgcg acgaccggag ccgacgggcg gtggacggtc gcccacctgc
     9301 cggacgggcc ctacaccctc gtcttcagcg ctccggggca ccagccgacc gcgcatgccg
     9361 tccagttggg cgggggcggc gccggggcgg atgcccgggg cgatacgcgc caccaggagg
     9421 tgcggctgcg gcccgccgcc accgtgcgcg gtacggtccg cgggccggac gggcacccct
     9481 tggcggacgc cgccgtgacc ctgctccagg acgggacggt cgccggccac gcggtcaccg
     9541 ggccggatgg cgcctatgcc ttcgcggacc tggcgggacg ccactacacc ctctcggcgg
     9601 ccggctatcc gccgcacgcc gcgccgatat ccctggcggg cggcgcggac gcgcgactgg
     9661 acctggagtt ggcgcagccg ggcggggccg ggcagcggga gcgctgagag tgccgcccgt
     9721 ggcgtgcgcc ggcccgcggt gattcctcgt cagcgcggag ggtcgctccc gacgaccgtg
     9781 ccgacgcagg cagcgccggc ggcaccgctg ccggcggcgt tcgcacagtt ctgacgaggc
     9841 gtcactcatt ctccgcggtg cgccccgtca gcaagagcgc ccgggtttcc gccagttcgg
     9901 actggaggac gtcgccggcg gtgtcggcgt cgccggcggc cacggcctcg acgagggcgg
     9961 cgtggcggcc gtccccgtgg tgcgggtcgt ggtcgcgcag gccgagcagt cggacgaggt
    10021 cgatcaggcc ctggcgcagg gcggggacga actggccgaa gaggtcggtt agtacggggt
    10081 tgtgggcggc gtccaccacc gccgcgtgca gggcgatgtc cgcgtcgacg aaggccgcgt
    10141 cgtcggcgcc gtccgcccgc cgcctctcca gggccgcgta cagggccgtg acatcggcct
    10201 cggtgcggcg ctcggcggcg aggcgggcgg cctggacctc gaccatcgcg cgcacctcat
    10261 agacgtcggc gacggccgcg cgccgcaggc gggtcggcca gtcctcggtg ggttcggcgg
    10321 cgatgacgaa gacgccggcg ccctggcggg gctgcaccat tccggcgccg gccagcgcgc
    10381 gcagcgcttc gcggaccgtc gagcggccga cgccgagggt cttggcgagc gtcgtctcgc
    10441 ccgggagcct gctgcccacc ggccagtgtc cgccggtgat ctggtcgcgc agctgttgcg
    10501 cggcctgttc gaccaggggg ctggggcgga gggcttccag cggcatcccg tttctcctgt
    10561 cctcgtaacc cctcagcttg tctgaggagt tgattcatgg ttagtgtacc cgccatgacg
    10621 ctgcgtggcc tcctcctcgg gtgccgcggc ggggcctgac gcgaccggca ccccgccgcg
    10681 gggtccggtg ctgccggtct ccccccggct tctgcgaagg aacgcgatgt cccgcacttt
    10741 ccccaccgtc cgcacgcccc gcgggccggt gcccgcgacc gccccggcct ggaatccgca
    10801 gcgcggcagc gccatgccgg cccaccgcta ccggcccgcc caccagcggg tcgaggtgcc
    10861 gcacaccgag cgcgagtggc ccgccgcccg gatcgagcgg gcgcccctgt gggtgccggt
    10921 cgacctgcgc gacggcaacc aggcactggc cgaaccgatg gacaccccgc gcaagcgccg
    10981 gctgttcgac
//
