LOCUS       NZ_JAPTYM010000001     11297 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Streptomyces noursei strain ATCC 12757
            contig_1_pilon_pilon_pilon_pilon, whole genome shotgun sequence.
ACCESSION   NZ_JAPTYM010000001
VERSION     NZ_JAPTYM010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10059690)
  AUTHORS   Koduru,L. and Wong,F.T.
  TITLE     Cost-effective hybrid long-short read genome sequencing and assembly
            protocol for GC-rich Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10059690)
  AUTHORS   Koduru,L. and Wong,F.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2022) Institute of Molecular and Cell Biology,
            A*STAR, 61 Biopolis Dr, Proteos, Singapore 138673, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-AUG-2022
            Assembly Method                   :: Flye v. 2.8; Pilon v. 1.24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026913165.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 08:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,375
            CDSs (total)                      :: 9,278
            Genes (coding)                    :: 8,845
            CDSs (with protein)               :: 8,845
            Genes (RNA)                       :: 97
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 433
            CDSs (without protein)            :: 433
            Pseudo Genes (ambiguous residues) :: 0 of 433
            Pseudo Genes (frameshifted)       :: 168 of 433
            Pseudo Genes (incomplete)         :: 311 of 433
            Pseudo Genes (internal stop)      :: 17 of 433
            Pseudo Genes (multiple problems)  :: 60 of 433
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:08:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7169263
            Orig. end                         :: 7180560
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPTYM010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="29"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            429..1166
                     /gene="hemQ"
                     /locus_tag="O1M07_RS31460"
                     /old_locus_tag="O1M07_31455"
     CDS             429..1166
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="O1M07_RS31460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31455"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_189862682.1"
                     /transl_table=11
                     /translation="MDDMTDASTPASDKIPNAGKKAKDLNEVIRYTLWSVFKLRDVLPE
                     DRSGYADEVEELFAQLAAKDVVVRGTYDLSGLRADADVMIWWHSENSDDLQEAYNRFRR
                     TRLGRAMEPVWSNMALHRPAEFNKSHIPAFLADEHARDYVSVYPFVRSYDWYLLPDEDR
                     RRMLADHGKMARGFPDVRANTVASFSLGDYEWILAFEADELHRIVDLMRHLRGSEARRH
                     VREEIPFYTGRRKPVAELVAGLA"
     gene            complement(1243..2952)
                     /locus_tag="O1M07_RS31465"
                     /old_locus_tag="O1M07_31460"
     CDS             complement(1243..2952)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (91-501). E-value:
                     3.3e-17. Score: 54.9. Matches aSDomain:
                     nrpspksdomains_O1M07_RS31465_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536946.1"
                     /locus_tag="O1M07_RS31465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31460"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_229921884.1"
                     /sec_met_domain="PF00561 (E-value: 6.9e-21, bitscore: 74.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGALGSLVLTGLASAPAGGAAPDPAAPPAPVHPLTFGRC
                     AGVEHLPASVECGTLTVPLDYARPRGKQIKLTVSRTRATSSAERQGALVFNPGGPGASS
                     MQFPLYGALPTWHRTARAYDFVGYAPRGVGRSAPLSCQDPKEFTKAPTDSPRYPSESFK
                     QKKVAEAKAYAHGCAQRAGADLPFYNSVNNARDLDMLRAALGEKKLTFMGASYGTYFGA
                     VYATLFPGHVRRMVFDSVVNPDPRQIWYQNNLDQSVAFERRWGDWLRWVAKHDATYHLG
                     ADKQAVQHSYDTVIQRLRHAPVNGKIGPGQLQAAYLKTGYNDAFWPMRAEALSKYLRGD
                     AQLLIAQALPSGDGQGEENANAVYTAVECNDSAWPRNWHVWDRDNTALARTAPFETWDN
                     AWMNLPCAFWPERRQSAQAGVEEAIRTTTQRAVKGGSAGDALAHASQQAADTDLDDALY
                     GRGRPLDIRTEPGALPPVLLLAAERDAATPYPGALELQRRLPGSSLVTERAAGTHGIAY
                     GENLCVNAYVETYLLRGQVPGHRSYCGPRAEPVPGQPLQRARPTLVQQVKGR"
     aSDomain        complement(1450..2679)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O1M07_RS31465_Abhydrolase_1.1"
                     /evalue="3.30E-17"
                     /label="O1M07_RS31465_Abhydrolase_1.1"
                     /locus_tag="O1M07_RS31465"
                     /protein_end="501"
                     /protein_start="91"
                     /score="54.9"
                     /tool="antismash"
                     /translation="ALVFNPGGPGASSMQFPLYGALPTWHRTARAYDFVGYAPRGVGRS
                     APLSCQDPKEFTKAPTDSPRYPSESFKQKKVAEAKAYAHGCAQRAGADLPFYNSVNNAR
                     DLDMLRAALGEKKLTFMGASYGTYFGAVYATLFPGHVRRMVFDSVVNPDPRQIWYQNNL
                     DQSVAFERRWGDWLRWVAKHDATYHLGADKQAVQHSYDTVIQRLRHAPVNGKIGPGQLQ
                     AAYLKTGYNDAFWPMRAEALSKYLRGDAQLLIAQALPSGDGQGEENANAVYTAVECNDS
                     AWPRNWHVWDRDNTALARTAPFETWDNAWMNLPCAFWPERRQSAQAGVEEAIRTTTQRA
                     VKGGSAGDALAHASQQAADTDLDDALYGRGRPLDIRTEPGALPPVLLLAAERDAATPYP
                     GALELQRRLPG"
     misc_feature    2906..2925
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 23.45"
                     /tool="antismash"
     gene            complement(3089..3865)
                     /locus_tag="O1M07_RS31470"
                     /old_locus_tag="O1M07_31465"
     CDS             complement(3089..3865)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536945.1"
                     /locus_tag="O1M07_RS31470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31465"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_189862684.1"
                     /transl_table=11
                     /translation="MWLLFLLLAWVAAVVSCVRVCRTAVVTGQARHRAQGTPPDTDDAH
                     AGLTLYETAYLSGGPHRLADVVLVLMAQQRRLHLAHTGWATVVDPVGQDPVERAALTAI
                     GPEGQCRIPAVRTALSGTDAVRGLADRLVDAGLAAPAAIRTAVRQVRGAALVVLLAAGA
                     TYWTAPSGEPDRLVLAWFALPLILTLGTWAIARAESRPSTGWATEAGAARLRQEHPGAR
                     DRRPAPAPAGLTGLALHGPAVLVDPGLRAALHSSGA"
     misc_feature    3908..3922
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            complement(4003..4992)
                     /locus_tag="O1M07_RS31475"
                     /old_locus_tag="O1M07_31470"
     CDS             complement(4003..4992)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="O1M07_RS31475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31470"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_189862686.1"
                     /transl_table=11
                     /translation="MSFTQGVYLGLGLSSLALLVGALVVRLRTPAVGRAAGPTRDVREL
                     AFLAGGPGRVADTALWVMHKDRRLTIGGPGVVTVRRATAHNPVETAVLDAVATTPGGAL
                     AAVRGRVMRSAAVQEVGDRLAARGLLRPPRAGRGWRLLAGLQVKASVVLFAVALVLSFL
                     AVRDGGGPWAPPVAVRVVPAVAVGLLVGLPCRRLFTRRITRAGRSALAAARPRGTRPDG
                     LRGLEAARQVAGLAVALGGAVVLTDALLRDHFVQAQGAVAASTPSGGSSAGSGGCGGDA
                     GSAWCGSSDAGGDSGCGASSCGGGSSCGGSSGCGGSGCGGGGCGGGGD"
     gene            complement(5001..6297)
                     /locus_tag="O1M07_RS31480"
                     /old_locus_tag="O1M07_31475"
                     /pseudo=""
     CDS             complement(5001..6297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675658.1"
                     /locus_tag="O1M07_RS31480"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31475"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 1e-94, bitscore: 315.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIAAEIAALPGVDWVEVVAENVCPGHLPDALRE
                     LRERGTTVIPHGVSLGLGGADRPDTGRLAALAERATALEAPLVTEHIAFVRAGGPLTAS
                     PVLEAGHLLPTPRTRDALDVLCENVRIAQDALPVPLALENIAALFAWPGEELTEGQFLS
                     ELVERTGVGLLIDVANLHTNHVNRGEDPTAALDELPVEALAYVHVAGGVERDGVWHDSH
                     AHPVTRPVLDVLAELCARTDPPGVLLERDDDFPEGGELAGELDAIRAVVEEARRVRAA"
     gene            complement(6454..7092)
                     /locus_tag="O1M07_RS31485"
                     /old_locus_tag="O1M07_31480"
     CDS             complement(6454..7092)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="O1M07_RS31485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31480"
                     /product="LysE family translocator"
                     /protein_id="WP_189862688.1"
                     /transl_table=11
                     /translation="MLTSLIGFTVVAALLTVSPGPDFAVVLRTALGSGRRAALCSALGI
                     AAGCFVWGLAGAVGLTAMLAASQTAYDALRVTGASYLVWLGVQALRGARRRRGADGPAD
                     EAAPAVPGSPWRAFRGGLLTNVLNPKVGVVYLSLLPQFIPHGAPVVGTTLLLVAVHAVL
                     GVLWLGGVALAVHRARAVLQRPRVRRRLDQATGGVLVALGAAVGWEIAR"
     gene            complement(7197..7850)
                     /locus_tag="O1M07_RS31490"
                     /old_locus_tag="O1M07_31485"
     CDS             complement(7197..7850)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350473.1"
                     /locus_tag="O1M07_RS31490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31485"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_189862690.1"
                     /transl_table=11
                     /translation="MDGTDPKTPRERYRAQVRAEIKEHAWEQIATAGVSALSLNAVAKQ
                     MGMSGPALYRYFASRDELITALVKDAYQSLAEALRTASVGGADLAVLAHALRGWALADP
                     QRYFLVYGTPVPGYHAPADTTGIADQIMTVLLDACTALAADEPTTPFGAHLEAHREWVH
                     DHPAPPAALHKALTFWSRLHGVLSLELAGHFSGMGLDPALFFAAELDSLLAPGA"
     misc_feature    complement(7892..7905)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 11.95"
                     /tool="antismash"
     misc_feature    7894..7910
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.71"
                     /tool="antismash"
     misc_feature    complement(7941..7957)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 18.14"
                     /tool="antismash"
     gene            7979..8635
                     /locus_tag="O1M07_RS31495"
                     /old_locus_tag="O1M07_31490"
                     /pseudo=""
     CDS             7979..8635
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566587.1"
                     /locus_tag="O1M07_RS31495"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31490"
                     /product="SRPBCC family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MWRAARTAAGSTGNAFAESDRAPRQPTRAQQQARAQQPTRGTPCP
                     ASPVPVPASAPAQTLVPAPTPALAHAPLRTIWKL"
     gene            complement(8932..9641)
                     /locus_tag="O1M07_RS31500"
                     /old_locus_tag="O1M07_31495"
                     /pseudo=""
     CDS             complement(8932..9641)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088892.1"
                     /locus_tag="O1M07_RS31500"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31495"
                     /product="peptidyl-tRNA hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDDQRAQAPTQPAPEYVLPLVVRVERAAPPGARTRWRPRRGRCWC
                     CSPTNGPPTRASGPGRCTTGRTPGYGRWSGGRAERSGGGPRPCPASRSPAARPRCGSSR
                     RCRWTAGRRTSPGSRSPAPSWTNRRPPRSPRTARRCCGSIPG"
     gene            9864..>10769
                     /locus_tag="O1M07_RS31505"
                     /old_locus_tag="O1M07_31500"
                     /pseudo=""
     CDS             9864..>10769
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088893.1"
                     /locus_tag="O1M07_RS31505"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_31500"
                     /product="ATP-binding cassette domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRLDGVGRRYGLRGPWVLREVRLDVPAGALLRIEGTNGSGKSTLL
                     RLLAGIDRPTTGRITGRPRTAYVPERFPAALPFTAAGYLAHLGRVHGLRTPEAADRAAE
                     WLARFGAAGHARTPLAELSKGTSQKVAVAQALLAEPELLVLDEAWTGLDPAARDTLDRA
                     VAERVAAGGTVVFVDHDPRRLAGAASARYRVADGRLLPREPGTAAEPTGGVDAGAGPRT
                     VVEAEGPPGSPPPPGLPGAPDLAPGPGNSLRLTVATVHSDALLRALLTARPPWHIRAVA
                     AEPPGPGTPGTPGTPAPGEAP"
ORIGIN
        1 gacggccacc ggatcaaggc gtcgaccttc gccagccaca agtggaactg gatcgccgag
       61 gccgacccgg acctcttcgt gctgcgcacc tccctcggcc ggtacggcga cgaggccgat
      121 ctggagcgcg acgacgccga actggtcgcc ctctccctcg ccgacctcgg cgaggcggtc
      181 ggcctgaccg cccggccggt gtccaccacc gtcacccgct gggacggcgg gctgccgcag
      241 tacgccgtcg gccatgtgga ccgggtggcg cggatccggg ccgcggtcgc cggactgccg
      301 ggcccgctgc gggtctgcgg cgcgctctac gacggcgtcg gcatcccggc gtgcatcgcc
      361 tccgcgcacc gggccgccga cgaggtcctc gggaccctga cggcgctcgc cggcggcggc
      421 gagtgagaat ggacgacatg acagacgctt ccacccccgc ctccgacaag atccccaacg
      481 ccggcaagaa ggccaaggac ctcaacgagg tcatccgcta caccctctgg tcggtcttca
      541 agctgcgcga cgtcctcccc gaggaccggt ccggctacgc cgacgaggtc gaggagctgt
      601 tcgcccagct cgccgccaag gacgtggtgg tccgcggcac ctacgacctc tccgggctgc
      661 gcgccgacgc cgacgtgatg atctggtggc actcggagaa ctccgacgac ctccaggagg
      721 cgtacaaccg cttccgccgc acccgtctgg gccgcgcgat ggagccggtc tggtcgaaca
      781 tggcgctgca ccgtcccgcc gagttcaaca agtcgcacat cccggccttc ctggccgacg
      841 agcacgcccg cgactacgtg agcgtctacc cgttcgtgcg ctcctacgac tggtacctcc
      901 tccccgacga ggaccgtcgc cggatgctcg ccgaccacgg caagatggcc cgcggcttcc
      961 ccgacgtccg cgccaacacc gtcgcctcct tctcgctcgg cgactacgag tggatcctcg
     1021 ccttcgaggc cgacgagctc caccgcatcg tcgacctgat gcgtcacctg cgcggctccg
     1081 aggcgcgccg gcacgtccgc gaggagatcc cgttctacac cggccgccgc aagccggtcg
     1141 ccgagctggt cgccggcctg gcctgaccgg tcggacaccc cggaacacgg aggtcggggc
     1201 cgcccccgaa gggacggccc cgacctccac gctccggggc ggctaccgcc ccttcacctg
     1261 ctgcaccagc gtcggccggg cccgctggag cggctgcccc ggcaccggct cggcgcgcgg
     1321 tccgcagtac gaccggtgcc cggggacctg cccgcgcagc aggtacgtct cgacgtaggc
     1381 gttcacacag aggttctcgc cgtacgcgat gccgtgggtg cccgcggccc gctcggtcac
     1441 cagggacgag ccgggcagcc gccgctgcag ctccagcgcg cccggatagg gggtggccgc
     1501 gtcccgttcg gcggccagca gcagcaccgg cggcagcgcg ccgggctcgg tgcggatgtc
     1561 cagcgggcgg ccccgcccgt acagcgcgtc gtccaggtcg gtgtcggcgg cctgctggga
     1621 ggcgtgggcc agggcgtccc cggccgaccc gcccttgacg gcccgctggg tggtggtgcg
     1681 gatcgcctcc tcgacgccgg cctgcgccga ctgccggcgc tccggccaga aggcgcacgg
     1741 caggttcatc caggcgttgt cccaggtctc gaacggcgcg gtgcgggcca gggcggtgtt
     1801 gtcccggtcc cagacgtgcc agttgcgcgg ccaggccgag tcgttgcact cgacggcggt
     1861 gtagacggcg ttggcgttct cctcgccctg gccgtccccg gagggcagtg cctgggcgat
     1921 caacagctgg gcgtcgccgc gcaggtactt cgacagcgcc tcggcgcgca tcggccagaa
     1981 cgcgtcgttg tagccggtct tgaggtacgc cgcctggagc tgcccggggc cgatcttccc
     2041 gttgaccggc gcgtgccgca gccgctggat gacggtgtcg taggagtgct gcaccgcctg
     2101 cttgtcggct cccaggtggt aggtggcgtc gtgcttggcg acccagcgca gccagtcgcc
     2161 ccagcggcgc tcgaaggcga cgctctggtc gaggttgttc tggtaccaga tctgccgggg
     2221 atcggggttg accacgctgt cgaagaccat ccggcggaca tggccgggga acagcgtcgc
     2281 gtagaccgcc ccgaagtacg tcccgtagga cgcgcccatg aacgtcagct tcttctcgcc
     2341 gagcgccgcc cgcagcatgt ccaggtcccg ggcgttgttg accgagttgt agaacggcag
     2401 gtcggcgccg gcccgttggg cgcagccgtg ggcgtacgcc ttcgcctccg ccaccttctt
     2461 ctgcttgaag gactcgctgg ggtaccgcgg gctgtccgtg ggtgccttgg tgaactcctt
     2521 ggggtcctgg caggacagcg gggcgctgcg ccccaccccg cgcggcgcat agccgacgaa
     2581 gtcgtaggcg cgggcggtcc ggtgccaggt cggcagcgcg ccgtagaggg ggaactgcat
     2641 gctggacgcg ccggggccgc cggggttgaa gaccagggcg ccctgccgtt cggcggacga
     2701 ggtggcccgg gtccggctga ccgtcagctt gatctgcttg ccgcgcggcc gggcgtagtc
     2761 gagcgggacg gtgagcgtgc cgcactcgac ggaggccggg aggtgctcca cgcccgcgca
     2821 ccggccgaag gtgagggggt gaacgggggc gggcggcgcc gccgggtccg gggcggcgcc
     2881 gccggccggt gcggaggcga ggccggtcag gaccagcgac ccgagggcgc cgtacaacgc
     2941 aagtgctctc acgagccgtc ccttcgtatg gtgcgcggtg cggggctccg tggcggggct
     3001 cgctgcacgg ggtcgcacgg tgggcataca aaagggatag ttgggccaac cgagggcgaa
     3061 gtaaagcacc gtacgacggt gtcggacgct atgccccgga agagtggagc gccgcgcgca
     3121 aaccgggatc gacgagcacg gcgggtccgt gcagggcgag tcccgtcagg ccggccggtg
     3181 ccggcgccgg acgtctgtcc cgcgcgccgg ggtgctcctg ccgcagccgt gccgcgccgg
     3241 cctcggtggc ccagccggtg gacgggcggg actccgcgcg ggcgatggcc caggtgccga
     3301 gggtgaggat cagcgggagc gcgaaccatg ccagcaccag ccggtcgggt tcgccggacg
     3361 gggccgtcca gtaggtcgcg ccggcggcca acagcaccac gagcgcggcg ccgcggacct
     3421 ggcggaccgc ggtgcggatc gcggcgggcg ccgcgagacc ggcgtcgacc agccggtcgg
     3481 ccagcccccg gaccgcgtcg gtgccggaca gggcggtccg gacggcgggt atgcggcact
     3541 gcccctcggg gccgatggcg gtgagcgcgg cccgctcgac ggggtcctgg ccgaccgggt
     3601 cgacgaccgt ggcccagccg gtgtgggcga ggtgcagccg ccgctgttgg gccatgagga
     3661 cgagcaccac atcggcgagt cggtgcgggc cgccggagag ataggccgtc tcgtacaggg
     3721 tcagcccggc gtgggcgtcg tcggtgtccg gcggggtgcc ctgggcgcgg tgccgggcct
     3781 ggccggtgac gaccgccgtg cggcagaccc ggacacagga gacgaccgcc gccacccagg
     3841 cgagcaggag gaagaggagc cacatagcgg acgttctacg ggacggcggg cgcggccgcc
     3901 atgatgcttc tcaccctgtg aatatttcgc cccggccggc cggggcgccg aactcgccgc
     3961 cctgaccagc cgcttcgcgc ccacacctga cctgacggtt cgtcagtcac cgccgccgcc
     4021 gcagcccccg ccgccgcacc cggagccccc acagcccgaa ctcccgccgc acgacgaccc
     4081 gccgccgcag gaggacgccc cgcatccgga gtccccaccg gcgtccgagg agccgcacca
     4141 cgccgagccc gcgtccccgc cgcacccgcc ggagcccgcc gacgacccgc cggacggtgt
     4201 cgaggccgcc accgccccct gggcctgcac gaagtggtcc cgcagcaacg cgtccgtgag
     4261 caccacggca ccgcccagcg ccaccgccag cccggcgacc tgcctggccg cctccaggcc
     4321 ccggaggccg tcggggcggg tgccccgggg ccgggccgcg gccagggcgg accggcccgc
     4381 ccgggtgatc cggcgggtga acaaccgccg gcacggcagc ccgaccagca ggcccacggc
     4441 caccgccggg acgacccgga cggccaccgg cggcgcccac ggaccaccgc cgtcgcggac
     4501 ggcgaggaag gacagcacca gcgcgacggc gaagagcacg acgctcgcct tcacctggag
     4561 cccggccagc agccgccagc cccggccggc gcgcggcggg cgcagcagcc cccgggccgc
     4621 cagccggtcg ccgacctcct gcaccgccgc actgcgcatc acccgcccgc ggacggcggc
     4681 cagcgccccg cccggggtgg tggcgaccgc gtccagcacg gccgtctcca ccgggttgtg
     4741 cgcggtggcg cgccgcaccg tcaccacacc cggaccgccg atcgtcagcc gcctgtcctt
     4801 gtgcatcacc cagagcgcgg tgtccgccac ccggcccggc ccgccggcaa ggaacgccaa
     4861 ctcccggacg tcacgggtcg gtccggccgc ccgcccgacc gccggcgtcc gcagccgcac
     4921 caccagggcc ccgacgagca gcgccagcga cgacaggccg aggcccagat acaccccttg
     4981 cgtgaacgac acggcgcacc tcacttccgg cgcagcgcgc gctgcagggt ccctgcccgc
     5041 cgggcggcgg cccgcgcgag ccgcgccgcg cggcgcccgg acgacggccg cggaccggcc
     5101 cggtcgcgcc accagtgcgt cagccgccgc cgtgccaccg ggtccgcggg gcgatcctgc
     5161 gccagcagga acgccgcgaa gtccagggcg tcctggcggt agccgccggc catggggcgg
     5221 cgccgggcgt agcggaggaa ggccgaccgg tagccgtcgc cgaggatctc cgtcagctcc
     5281 ggtgccacct tggcgaccac cgcggcccgc ttggcggcca gggcccggct ctgcacccgc
     5341 agccgggccg ggtcgaagcc ctccggggcg ggcgtcccgg ccaccagtgc ggacagcagc
     5401 gcggcctgcg ccagcgccaa ccgctcacgg gccggaccgg tgtccgccac tgcctcaagc
     5461 cgcccggaca cggcgcgcct cctccaccac ggcgcggatc gcgtcgagtt caccggccag
     5521 ctcgccgccc tcggggaagt cgtcgtcgcg ctccagcagc acgccgggcg gatcggtccg
     5581 ggcgcacagc tccgcgagga cgtccaggac cggccgggtc accgggtggg cgtggctgtc
     5641 gtgccacacc ccgtcccgct cgacaccgcc cgcgacatgg acgtaggcga gcgcctcgac
     5701 cggcagctcg tcgagggccg cggtcgggtc ctcgccgcgg ttgacgtggt tggtgtggag
     5761 gttggcgacg tcgatcagca gcccgactcc ggtgcgctcg accagctcgg acaggaactg
     5821 cccctcggtc agctcctcgc cgggccaggc gaacagcgcg gcgatgttct ccagggccag
     5881 cggcaccggc agtgcgtcct gggcgatccg gacgttctcg cacagcacgt ccagcgcgtc
     5941 ccgggtgcgc ggcgtcggca gcaggtgccc ggcctccagc accggcgacg cggtcagcgg
     6001 gccgccggcg cggacgaacg cgatgtgctc ggtgaccagt ggggcctcca gcgcggtggc
     6061 ccgctcggcg agggccgcca gccgcccggt gtccggccgg tccgcgccac cgagcccgag
     6121 cgagaccccg tgcgggatca ccgtcgtgcc gcgttcccgc aactcccgca gggcgtccgg
     6181 gagatggccg gggcagacgt tctcggccac cacctccacc cagtccaccc cgggcagcgc
     6241 ggcgatctcc gcggcgatct ccggccgcca gccgatgccc gtccccaaac gacgcatggt
     6301 tccccctttg tgctccccgc ggtcggggta gtcctgaccc ggcgcggtcg gggcgaatcc
     6361 cgcacccgcg cggttcagag gttgatttga gcttctctca ccaatggccg gcccggatcg
     6421 tgccgtgcgc cggccgttgc ggcccgccgc ggctcagcgg gcgatctccc accccaccgc
     6481 cgcgcccagc gccaccagca ccccgccggt ggcctggtcc agccgccgcc gcacccgcgg
     6541 acgctgcagc accgcccgcg cccggtgcac cgccagggcc accccgccca gccacagcac
     6601 acccagcacc gcgtgcacgg ccaccagcag cagcgtcgtg ccgaccaccg gcgcgccgtg
     6661 cgggatgaac tgcggcagca gggagaggta gaccaccccc accttggggt tgaggacgtt
     6721 ggtcagcagc ccgccgcgga acgcccgcca ggggctgccc ggaacggccg gggcggcctc
     6781 gtccgccggc ccgtccgcac cgcgccgacg ccgggcgccg cgcagcgcct gcaccccgag
     6841 ccacaccaga tacgacgcgc cggtcacccg cagcgcgtcg tacgccgtct gcgaggcggc
     6901 cagcatcgcg gtcagcccca ccgccccggc cagtccccac acgaagcagc cggcggctat
     6961 gcccagcgca ctgcacagcg cggcgcgccg ccccgagccg agcgcggtcc gcagcaccac
     7021 cgcgaagtcc gggccggggg agacggtgag cagtgcggcc accacggtga agccgatgag
     7081 cgaggtcagc atgggtgaca cggtatgtcc cgccgcgggc cgggccgccg gcggggacgg
     7141 ggagcggtcc aacggggccc gccgccggcg gcccggccgt cgccccgcgt acgacgtcac
     7201 gctccggggg ccagcaggct gtccagttcg gccgcgaaga acagggcggg gtccaggccc
     7261 atgccgctga aatggccggc gagctccagg gacaggacac cgtgcaaccg ggaccagaag
     7321 gtcagggctt tgtggagagc cgccggtggg gcggggtggt cgtggaccca ttcccggtgc
     7381 gcttccaggt gggcgccgaa cggtgtcgtc ggttcgtccg ccgcgagggc tgtgcaggcg
     7441 tcgagcagga cggtcatgat ctggtccgcg atgccggtgg tgtccgcggg ggcgtggtaa
     7501 ccgggcacgg gggtgccgta gacgaggaag taccgctggg ggtccgccaa ggcccagccg
     7561 cgcagggcgt gcgccagcac ggccaggtcg gcaccgccga ccgaggcggt gcggagggcc
     7621 tcggcgaggc tctggtaggc gtccttgacg agtgcggtga tcagctcgtc gcggctggcg
     7681 aagtagcggt agagcgcggg tccgctcatg cccatctgtt tggcgaccgc gttgagcgag
     7741 agcgcggaca cccccgctgt ggcgatctgc tcccaggcgt gttccttgat ctccgcgcgc
     7801 acctgggcgc ggtagcgctc ccgaggggtc ttcgggtccg tcccgtccat gacctgccgc
     7861 cttccccgcc cgctcgccta tgccctcaag atttcgttag aacctatcac gcgatccctt
     7921 ggccgctcgc tctccagtta gttatatctt ctaacgaaca gctaaggatc taacggtcgt
     7981 gtggagggcg gcgcgcaccg ctgcaggttc aacaggcaac gccttcgcgg aatccgaccg
     8041 ggcgcctcgg caaccgacga gagcccaaca gcaggcgaga gcccagcaac cgacaagggg
     8101 aaccccatgt ccggcatcgc cagtcccagt cccagcctca gccccggcgc agacgctggt
     8161 acccgcaccc acgcccgcac tcgcccacgc gccgttgcgc accatctgga agctgtagac
     8221 cgacgtggtg cgctggccgt cctggcaggc cggcgtcagc accatgcaac gcctcgacca
     8281 cggccccttc cggaagggtt cggctttccg ttggacgccc ccggtaccgc ccaaccccac
     8341 gactcccgcc accgagttgg agatcacctc gaccgtccga cagctcaagc gcaactcctg
     8401 catccgctgg accggccccg cggtcggcgc gggactgcgc atcgacggcg tccacgtatg
     8461 gaacttcacc gaggccgagg gaggggtccg ggtgagcacc gaggagaccc acaccggcgc
     8521 ccaggtcgat gcgaacgttc ccgtcgcgac cgcgatcctc cgccagggcc tcgaagcatg
     8581 gttgcgcgag ctgaagacca ccgccgaagc gaccaccatc gagcagcggc actgaacccc
     8641 gacgcgccga ttccgtatcg ggtactgaac tcgccgtcgg gatcggtcga ttcgcggccg
     8701 atgacgcgga tgcgcagggt ggtgccgggc tgccgacgag tgccaactcg gtgctgtgct
     8761 cgtgagaagg cagggtggga catggccgcg cgagcccact tctcggtcac gcttggtcac
     8821 acgtgaagaa gccgcgcggc cttcgtctcg tcacggcgtc acccgcgttc ggcgccctcg
     8881 gcgacgttac cgccgtcgca gccgcccccc gtcatgaccc gtcggacaga cctagggccg
     8941 ccgtgtgcgg gggagagggc agaatcgact gcccccttcg gtcaccacgg ttttcccggg
     9001 cgcgatctcg gtgaacccgg cgtcccgcac cacgggcagt ccgctggtcg tcagtgccgg
     9061 ccaggccgcg ggcgtcgcgg tgcgcacggc cagcgggaac cctgccgcgc gccaggcttc
     9121 ccgctccgcc gtggacagct cccaccaggc cagctgtgcg ccgtgcccgg cctgtgccat
     9181 cgccttgccc gccgacatct tcagcccggg attgagccac agcaccggcg cgccgtccgc
     9241 gggctccgcg ggggcctgcg gttcgtccag ctcggtgccg gagacctgga gccgggcgag
     9301 gtccttcggc cagccgtcca gcggcaccgg cgggaagacc cgcacctcgg cctcgcggcc
     9361 ggtgaccgtg atgccgggca gggcctcggc ccgccgccac tccgctccgc gcgcccgccg
     9421 gaccaccttc cgtatccggg agtcctgcca gtcgtgcacc gccctggccc actcgcccgc
     9481 gtcggtggcc cgttcgtcgg agagcagcac cagcaccgcc cgcgccgcgg tctccagcgc
     9541 gtccgtgcgc ccgggggtgc ggcccgctcc acgcgcacca ccagcggcag gacgtactcc
     9601 ggcgccggct gcgtgggggc ctgcgcccgc tggtcgtcga tccgggtggg cgtgctgtgc
     9661 tcgccggacg ggccggcggt ctcggtactg gtcacccgat cagtctgcca gccgccaccg
     9721 acagtccccc ctcgcgaccg ccgacggtcg ccgaacccgc gcccgacgac gcggtgttgc
     9781 ccgcgcccgg ctaccgtcgg cggctacagg ggagcagggg agtgggggag gtgcggccga
     9841 tgccgcggca ggaagcgcag cggatgcggc tggacggggt cgggcggcgc tacggcctgc
     9901 gcgggccctg ggtgctgcgc gaggtgcggc tcgatgtgcc ggcgggcgcg ctgttgcgca
     9961 tcgaggggac caacggcagc ggcaagtcca cgctgctgcg gctgctcgcc ggcatcgacc
    10021 ggcccaccac gggtcggatc accgggcgcc cgcgcaccgc ctacgtgccc gagcggttcc
    10081 cggccgccct gccgttcacc gccgccggct acctcgccca cctcggccgc gtccacggcc
    10141 tgcggacacc ggaggcggcg gaccgggccg ccgagtggct ggcgcgcttc ggcgccgccg
    10201 ggcacgcccg gaccccgctg gccgagctgt ccaaggggac gagccagaag gtcgcggtcg
    10261 cccaggcact tctcgccgag cccgaactcc tcgtcctgga cgaggcgtgg accgggctgg
    10321 acccggccgc ccgcgacacc ctcgaccgcg cggtcgccga acgggtcgcc gccggcggca
    10381 ccgtggtctt cgtcgaccac gacccgcgcc gactggccgg ggccgcgagc gcccgctacc
    10441 gcgtcgccga cggccggctg ctgccccggg agcccggtac cgccgccgag ccgaccggcg
    10501 gggtcgacgc cggtgccggc ccgcggaccg tcgtcgaggc ggagggtccg ccgggcagcc
    10561 cgccgccgcc cggcctgccg ggcgcgccgg acctcgcccc cggtcccggc aacagcctgc
    10621 ggctgaccgt cgccaccgtc cactccgacg ccctgctgcg cgccctgctc accgcgcgcc
    10681 cgccctggca catccgggcg gtcgccgccg agccgcccgg ccccggaacc cccggaaccc
    10741 ccggaactcc tgctcccgga gaggcccccg gccatgaccg cctccctgcc cgcgctggtc
    10801 cgctatcagg cggcgctgct cgtcggttcc taccgttggc tgccgccgct cctcgcctac
    10861 gcggcgttcc tcgcgatcgg cgtccagacc ggcggcccga tcctggacgc gtacggctac
    10921 gccgccggcg ggctgctgcc ggtctccgtc tggctggccc ggatctgtgc caccaacgag
    10981 ccgccggccg cccgcagttg catggcggcc gccgccggac ccggccgggc gcacctcgcc
    11041 gcgctgctgg ccgcggtcgg cgcggcgctg gtcctcgcac tcgccgggac ggccttcgtc
    11101 gcgctgctct ccgacccgca ctcgaccggt caccgggtcg cggtcccggt gctgcccgcg
    11161 accggcgccg ggctgctcac cgcgtcggtc tgcctcgtgc tgggcatcgg gatcggcgcg
    11221 ctgtgctccc cgccggtgct gcgccgcccc ggctggggcg tcccggtcgg gctgatcgcc
    11281 gcgctgctgg tcctcgt
//
