LOCUS       NZ_JAPTYM010000001     10216 bp    DNA     linear   CON 29-AUG-2024
DEFINITION  Streptomyces noursei strain ATCC 12757
            contig_1_pilon_pilon_pilon_pilon, whole genome shotgun sequence.
ACCESSION   NZ_JAPTYM010000001
VERSION     NZ_JAPTYM010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces noursei
  ORGANISM  Streptomyces noursei
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 10059690)
  AUTHORS   Koduru,L. and Wong,F.T.
  TITLE     Cost-effective hybrid long-short read genome sequencing and assembly
            protocol for GC-rich Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10059690)
  AUTHORS   Koduru,L. and Wong,F.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2022) Institute of Molecular and Cell Biology,
            A*STAR, 61 Biopolis Dr, Proteos, Singapore 138673, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-AUG-2022
            Assembly Method                   :: Flye v. 2.8; Pilon v. 1.24
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_026913165.1-RS_2024_08_29
            Annotation Date                   :: 08/29/2024 08:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,375
            CDSs (total)                      :: 9,278
            Genes (coding)                    :: 8,845
            CDSs (with protein)               :: 8,845
            Genes (RNA)                       :: 97
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 433
            CDSs (without protein)            :: 433
            Pseudo Genes (ambiguous residues) :: 0 of 433
            Pseudo Genes (frameshifted)       :: 168 of 433
            Pseudo Genes (incomplete)         :: 311 of 433
            Pseudo Genes (internal stop)      :: 17 of 433
            Pseudo Genes (multiple problems)  :: 60 of 433
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:08:00
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1477540
            Orig. end                         :: 1487756
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JAPTYM010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="11"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5216)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    504..520
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.57"
                     /tool="antismash"
     misc_feature    complement(735..749)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            complement(1687..3966)
                     /locus_tag="O1M07_RS06220"
                     /old_locus_tag="O1M07_06215"
     CDS             complement(1687..3966)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540973.1"
                     /locus_tag="O1M07_RS06220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_06215"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_189864242.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.2e-37, bitscore:
                     127.7, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPDAAAAPPQHLPPAGRGRHRPEGAPPTRRRRPPAAAPKAKKS
                     VRTPTVLQMEAVECGAASLAMVLAHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYG
                     LSAKGMQMEPAALAEVQAPAVLFWEFNHYVVYDGMGRRLGRRGVHINDPDKGRRFVPTE
                     DFDTSFTGVALVFEPTEAFRKGGRRPSVRSALPARLRGTAGTLLAALLASLLLVGVGAA
                     LPALSRTYIDMFLIGNQTSLLGPLFAAMSAMVVLTAVLTGLQQANLLRGRIISSTLSSA
                     RFLRHLLRLPVTFFSQRSPADLVQRLQSNDAVAETLARDLAAAAVDGVVVVLYAVLLWT
                     YDPQLTVIGVLIALLNVVAMRIVVRLRATHTQKLRADTARLTNTSYTGLQLIETMKATG
                     GENGYFRRWAGQHATTLEEQQRLGVPSAWLAVVAPTLATLNSALILWIGGLRAVEGHIS
                     IGLLVAFQALVTRFTAPITRLNGVAGRIQDFAADVARLKDVESFPADAHYSRPDADAGT
                     RRLKGHITLDGVTFGYSPLDAPLLTGFSLAVGPGRQVALVGGSGSGKSTVSRLISGLYT
                     PWEGTIRIDGQRLADIPRSALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDDAVIAAL
                     QDAALYDDVIARRPDGIHSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALD
                     AGTEAVIMDNLRRRGCACVIIAHRLSTVRDSDEIVVLDHGTVVERGRHEELVAAGGAYA
                     ELVKEH"
     gene            complement(3963..4769)
                     /locus_tag="O1M07_RS06225"
                     /old_locus_tag="O1M07_06220"
     CDS             complement(3963..4769)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540972.1"
                     /locus_tag="O1M07_RS06225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_06220"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_189864244.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRFARPQGWLVLTVTVLVMIAATVW
                     AVTGTVSATLTAPGILTHGRGSYVLQSPVAGQVTAVLAEEGQPVAAGAPLARIRTVQGT
                     TTVVRALAAGRLTSLVATIGAVVTTGADLAAVERTNGSRDPLTATLYVPATSAATLPAG
                     AAVDLTVASAPTQRYGVLRGHVAAIGRTAQSRQRISAFLGSSQLGEQFSRAGQPVAVLV
                     RLDTASTATGYAWSTSDGPPFALDSMTQATAAVHLAAQHPIDWLLS"
     misc_feature    4734..4746
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.37"
                     /tool="antismash"
     gene            complement(5001..5216)
                     /locus_tag="O1M07_RS06230"
                     /old_locus_tag="O1M07_06225"
     CDS             complement(5001..5216)
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576071.1"
                     /locus_tag="O1M07_RS06230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_06225"
                     /product="type A2 lanthipeptide"
                     /protein_id="WP_189864246.1"
                     /sec_met_domain="strepbact (E-value: 8.3e-22, bitscore:
                     76.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNAQVQTQEISDADLDNVSGGLVGGVLSTVTTTVGSVAPVGSVL
                     ATATGLVQQTTGLDVNGIVGGATAGL"
     misc_feature    complement(5234..5248)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.56"
                     /tool="antismash"
     misc_feature    5235..5248
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.98"
                     /tool="antismash"
     misc_feature    complement(5235..5249)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.62"
                     /tool="antismash"
     misc_feature    5236..5249
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.16"
                     /tool="antismash"
     misc_feature    complement(5588..5606)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.26"
                     /tool="antismash"
     misc_feature    5690..5707
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.91"
                     /tool="antismash"
     gene            5893..6933
                     /locus_tag="O1M07_RS06235"
                     /old_locus_tag="O1M07_06230"
     CDS             5893..6933
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018540970.1"
                     /locus_tag="O1M07_RS06235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_06230"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_189864248.1"
                     /transl_table=11
                     /translation="MDPVAALERIAFLLERQGAVTYRVQAFRTAASVLDGLAAEELRER
                     AGNGTLGRLKGLGPKTTRVVEEALAGRQPAYLTRLTEEAGGPLVEGERGLALRRALRGD
                     CHLHSNWSDGGSPVEAMARAARDLGHDWCALTDHSPRLTVAHGLTAERLRRQLDVVAEV
                     NARLAPFRLLTGIECDILDDGSLDQEPELLDRLDVVVASVHSKLRMDRRPMTRRMLAAV
                     GNPLVDVLGHCTGRQLTGRLRPESAFDAAEVFAACAEHRTAVEINCRPDRFDPPRRLLR
                     QALDAGTLFAVDSDAHAPGQLDWQIYGCARAEECGVPADRIITTWTARQLTTWTRTGRT
                     PHGAGG"
     gene            complement(6964..7872)
                     /locus_tag="O1M07_RS06240"
                     /old_locus_tag="O1M07_06235"
     CDS             complement(6964..7872)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="O1M07_RS06240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_06235"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_039638460.1"
                     /transl_table=11
                     /translation="MIEARRLHILRALADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVERGARGVRLTAAGDILLGHANAVLAQLERAEAELAAYDKGEAGTVTVAAFATGIA
                     LVLAPALAELAGSAPGIRVRVQDAEGDASLLMVLDRQVDVAVAVEYRGAPDADDPRLTR
                     VPLYAEPFDAVLPPGHPLAGAAAVPLAGLAKDPWIGPYSGNPCHDVVVLACEYAGFRPR
                     LTHSSDDFRAVVALASAGVGVALVPRSALRGMDLGGVVVRPVDGAPPTRRVFAAVRRGA
                     EDHPLFRPVLAALRTAASAQG"
     gene            complement(7924..9117)
                     /locus_tag="O1M07_RS06245"
                     /old_locus_tag="O1M07_06240"
     CDS             complement(7924..9117)
                     /EC_number="2.3.1.29"
                     /GO_function="GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (45-388). E-value:
                     6.6e-72. Score: 234.5. Matches aSDomain:
                     nrpspksdomains_O1M07_RS06245_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1109: 8-amino-7-oxononanoate synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999157.1"
                     /locus_tag="O1M07_RS06245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="O1M07_06240"
                     /product="glycine C-acetyltransferase"
                     /protein_id="WP_189864250.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.1e-69, bitscore:
                     234.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFDAVRDDLRTALDEIRAAGLHKPERVIGTPQSATVAVTAGGRPG
                     EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQEVHKELEKRISGFLG
                     QEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLSKARRLRYANRDMDD
                     LERQLKDAEGARRTLIVTDGVFSMDGYLAPLREICDLADRYGAMVMVDDSHAVGFVGPG
                     GRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQRSRPYLFSNSLAPV
                     IAAASLKVLDLLESAGDLRERLNANTALFRSRMTEEGFAILPGEHPIAPVMIGDAAEAA
                     RMAELLLERGVYVIGFSYPVVPKGEARIRVQLSAAHSTEDVHRAVDAFVAARAALAG"
     aSDomain        complement(7954..8982)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_O1M07_RS06245_Aminotran_1_2.1"
                     /evalue="6.60E-72"
                     /label="O1M07_RS06245_Aminotran_1_2.1"
                     /locus_tag="O1M07_RS06245"
                     /protein_end="388"
                     /protein_start="45"
                     /score="234.5"
                     /tool="antismash"
                     /translation="EVLNFCANNYLGLADHPEVIAAAHGALDRWGYGLASVRFICGTQE
                     VHKELEKRISGFLGQEDTILYSSCFDANGGVFETLLGPEDAVISDALNHASIIDGIRLS
                     KARRLRYANRDMDDLERQLKDAEGARRTLIVTDGVFSMDGYLAPLREICDLADRYGAMV
                     MVDDSHAVGFVGPGGRGTPELCGVMDRVDIITGTLGKALGGASGGYVAARAEIVALLRQ
                     RSRPYLFSNSLAPVIAAASLKVLDLLESAGDLRERLNANTALFRSRMTEEGFAILPGEH
                     PIAPVMIGDAAEAARMAELLLERGVYVIGFSYPVVPKGEARIRVQLSAAHSTEDVHRAV
                     DAF"
     misc_feature    complement(9316..9325)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    9381..9394
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
ORIGIN
        1 agcgcggcga gcgccagcgg cacgctgtag aggagcagca acacgaggtt gaccgcgccg
       61 accgtggcgg cctgaacggc caccggcccg acgccggcca gcacctggcg gatggcgctg
      121 atgcccatgg cggcactggc cagttcaccg gtggagcggc cggcgaagaa cttcgtcggc
      181 aggcgcagca gccggtccca gacggccggc tgcaggacgc tctcgatccg gccctcgatg
      241 cgcagcacgg tgaggttctg cagcagggtg aaggccgccg cgaccacgcc ggcgatgatg
      301 acggcgagcg cgacctggac gatcgggcca cgggcggcgg ccggtacgta gacgccgagg
      361 accttgccgg tggcgatcgg gacgagagcg ccgagggcga ccgtcaccag tccgctcagg
      421 gcgaggttcc gcaggtcggc gcggttcccg cggaggctga agcgcatcag ccgccacagc
      481 gtgagccggc gttcgggcag tggtcgatag aacatcaccg cgtgcggctc gaactcgtcg
      541 gcggtgtcct tgccgatccg gatccgcagc ccggaggcgg ggttgaccgc ctcgtaccgg
      601 ccgcgccgcc acaacagcgc caccggggca ccggacttgg cgcggtggcc caccaggggc
      661 ccggcgtcgg tgcgccacca gcggccgtcg aggcggaccg cgcgggtgcg gatccgggag
      721 gcgagggcga tccgttcgac ggggctgatc cggtcgccgg cggcggcgcc cttgggcggt
      781 tcggtgagca cgatgccggc ggccccggcg accttccggc agaccgcgaa ggtcaggtcg
      841 tcgccggtcc ggtcgcggcc cgcggcggcg cgtccggagc ggtccttgcc gatggacgcc
      901 agcagtgcct ggtcggcgcg ttcccggacg gcctcgccgg ccttgatgcc ggccgccgcg
      961 cggtcctcgt gggcgcgctc cagctgctcg atccagcggt cgacggcgga cagcagccgg
     1021 tactgctggt tgaccatccg ctgccacagc gccgcgtcga cgagcaggtc ggcggcggcc
     1081 tcggcgctgt aggaggcgcc gtactggacg ctgccgggcg gcaccggcat ccacaggacg
     1141 tcgtcgtcgg cgaccgcttc gtcgctgggc cggccgtcca acggcgcctc gaacagcacg
     1201 cccagaccgc gcgcggtgcc gagcgcgaag gcgtgctcca ggggggtggc cggctgcccg
     1261 ccgtaggtgt cgtcgggtcc gaaggtgccc tcgtgcccgt acgcgccctg gtcgccgtgg
     1321 tcggggcggg gcagttcgcg cagggcgatc cggcgcagtc cgcagtgctg ggaggggcgg
     1381 ccgacgaggg tgtgctgggg gccttggacg ggcccgagca ggagggtgcc ggcttcgagg
     1441 cggccgagga agtgccagtg gccttcctga ccggcgtcca ccgcgaacag gtcgagggtg
     1501 ccgccggtga ccagccacag cacgtgcggg ccttcgagcg ggacgtcgcg cagcccggcg
     1561 cagtcgacgg gggtgccgag gccgcccagg gcgtgcgtga ccgcgtcgac gccgtgcgga
     1621 tccgcggccg gtgcggccgg ctgcgtggcg gatcccgcgt ccggggacgg gtgtacggat
     1681 gccacgtcag tgctccttga ccagctcggc gtaggcgccc ccggcggcga ccagttcctc
     1741 gtgccggccg cgttcgacga ccgtgccgtg gtcgagcacc acgatctcgt cgctgtcgcg
     1801 gacggtgctc agccggtggg cgatgatgac gcaggcgcag ccgcggcgcc gcaggttgtc
     1861 catgatgacc gcctcggtcc cggcgtccag ggcgctggtg acctcgtcga ggacgaggat
     1921 gctggggcgc cggaccagcg cccgggcgat ctccaggcgc tgccgctggc cgccggagaa
     1981 gttgcggccg tcctgctcga cgcggctgtg gatgccgtcg gggcggcggg cgatgacgtc
     2041 gtcgtagagg gcggcgtcct ggagggcggc gatcaccgcg tcgtccggga tggaggggtc
     2101 ccacagcgcg acgttgtcgc ggacggtgcc ctcgaagagg aagacgtcct ggtcgacgaa
     2161 ggagaccgag gcggccagcg cgctgcgcgg gatgtccgcg aggcgctgtc cgtcgatccg
     2221 gatcgtgccc tcccaggggg tgtagaggcc ggagatcagc cgggagacgg tggacttgcc
     2281 gctgcccgag ccgccgacca gggcgacctg gcggccgggg ccgacggcca gggagaagcc
     2341 ggtcagcagc ggggcgtcga gcgggctgta gccgaaggtg acgccgtcga gggtgatgtg
     2401 gcccttgagg cggcgggtgc cggcgtccgc gtccgggcgg gagtagtggg cgtcggcggg
     2461 gaagctctcg acgtccttga ggcgggccac gtcggcggcg aagtcctgta tgcggccggc
     2521 gacgccgttg agccgggtga tcggtgcggt gaagcgggtc accagggcct ggaaggcgac
     2581 cagcaggccg atggagatgt ggccctcgac ggcgcgcagg ccgccgatcc acaggatcag
     2641 tgcgctgttg agggtggcga gggtgggcgc gacgaccgcc agccaggcgc tggggacgcc
     2701 gagccgctgc tgctcctcca gcgtggtggc gtgctggccg gcccaacggc ggaagtagcc
     2761 gttctcgccg ccggtggcct tcatggtctc gatgagttgg aggccggtgt aggaggtgtt
     2821 ggtcaggcgg gcggtgtcgg cgcgcagctt ctgggtgtgg gtggcgcgca gccggaccac
     2881 gatccgcatg gcgacgacgt tgagcagcgc gatcagcacg ccgatgacgg tgagctgggg
     2941 atcgtaggtc cacaacagca ccgcgtagag gacgaccacg acgccgtcga cggcggccgc
     3001 ggccaggtcg cgggcgaggg tctcggcgac cgcgtcgttg gactgcaggc gctggacgag
     3061 gtcggcgggc gagcgctggg agaagaaggt gaccggcagc cgcagcagat ggcgcaggaa
     3121 gcgggcgctg ctgagggtgg aggagatgat ccggccgcgg agcaggttgg cctgctggag
     3181 gccggtcagg acggcggtga gcaccaccat cgcgctcatc gcggcgaaca gcggccccag
     3241 cagcgaggtc tggttgccga tcaggaacat gtcgatgtag gtgcggctga gcgcgggcag
     3301 cgcggcgccg acgccgacca gcagcaggct ggcgagcagc gccgccagca gggtgccggc
     3361 ggtgccgcgc agccgggccg gcagggcgct ccgcacgctg ggccggcggc cgcccttgcg
     3421 gaacgcctcg gtgggctcga agaccagggc gacgccggtg aagctggtgt cgaagtcctc
     3481 cgtcggcacg aagcggcggc ccttgtcggg gtcgttgatg tggacgcccc ggcggccgag
     3541 gcggcggccc atgccgtcgt agacgacgta gtggttgaac tcccagaaga ggacggccgg
     3601 tgcctggacc tcggcgaggg cggccggctc catctgcatg cccttggccg acaggccgta
     3661 actgcgggcg gccttgagga ggttgctggc ccgggagccg tcgcgggaga cgccgcaggc
     3721 gatccgcagt tcctccagcg gcacatggcg tccgtagtgg gcgagcacca tcgccaggga
     3781 ggcggcgccg cactccaccg cttccatctg gagcacggtg ggggtgcgca cggacttctt
     3841 cgccttgggg gcggcggccg gcggcctgcg gcggcgggtg ggcggcgcgc cctcggggcg
     3901 gtgccgtccg cggccggcgg gcgggaggtg ctgcgggggt gccgcggcgg cgtcgggtgc
     3961 ggtcacgaga gcagccaatc gatcgggtgc tgcgcggcca ggtggacggc ggcggtggcc
     4021 tgggtcatcg agtccagggc gaacggcggg ccgtcggagg tggaccaggc atagccggtg
     4081 gcggtggacg cggtgtcgag ccggaccagc accgcgaccg gctggccggc gcgggagaac
     4141 tgctcgccga gctggctgct gccgaggaac gcggagatcc gctggcggct ctgggcggtg
     4201 cggccgatgg ccgcgacgtg gccgcgcagc acgccgtacc gctgggtggg ggcggaggcg
     4261 acggtgaggt ccacggcggc gccggcgggg agggtggcgg cgctcgtggc cgggacgtag
     4321 agggtggccg tcagggggtc gcgggagccg ttggtccgct ccacggcggc gaggtcggcg
     4381 ccggtggtga cgaccgcgcc gatggtggcg accaggctgg tcagccggcc ggcggcgagc
     4441 gcgcggacga cggtggtggt gccctgcacg gtccgtattc gcgccaacgg ggcgccggcg
     4501 gcgaccggtt ggccctcctc ggcgaggacc gcggtgacct ggccggcgac cgggctctgc
     4561 aggacgtagc tgccgcggcc gtgggtgagg atgccggggg cggtgagggt ggcggagacg
     4621 gtgccggtga ccgcccagac ggtcgcggcg atcatgacga ggacggtgac ggtcagcacc
     4681 agccagccct gggggcgggc gaagcgcacc ggcaggtcga gctcctcggg cgattgcagc
     4741 ttggaaaggg cctgttggcg gaactgcacg acactcttcc ctcaactgca ggggctggta
     4801 cgggagtagc gggagtgtac cgccgccgga gatccggtgg cggtttcccg gcggcgaaca
     4861 gccgggtgcc ccggacggcc cggccggaac cgggttgtcc ggggcacgcg gtacggcaga
     4921 tcgacaggga gggggcgccg actgccgtca cgaccgttcg tcgccgaccg tgccgcgcgg
     4981 cggcacaggg gcggctcgga tcagagaccg gcggtcgcgc cgcccacgat gccgttgacg
     5041 tcgaggccgg tggtctgctg gaccaggccg gtggcggtgg cgaggacgct gccgaccggg
     5101 gcgaccgagc caacggtggt ggtgacggtg ctcagcacgc cgccgacgag gccaccggac
     5161 acgttgtcca ggtcggcgtc ggagatttcc tgggtctgca cctgggcgtt gttcatgatg
     5221 tttcccttcg gatcaatatc aagaaaaaac aagcgaaacg ggccgtccgg tgggtgaccc
     5281 ggccgcggcc acggtagtcc ggcccccgat atcgggaaac cccggcgact tccgcggtgc
     5341 gatggatcaa agcatgcggg cgggtttccc tgctaatcgg atgagcggcg atccgggtgc
     5401 gactacgccg cgcaattacc ggacgtgtac gtgcgtccat ggcttgatcg aagcatcttc
     5461 acatctcctc cggtttcttt tccgtcgaag ggtcgttgcg acgcccccgg aatggggcat
     5521 tccggaacta gaccgtcgac cacctgggcg gccgtgtcac ccgaaaccct gaagcgtgta
     5581 ccggctgcgc attcggtgtg cagatttcct ggaggtcacc gaactcaacg tccggctgac
     5641 gcgccggccc gccgagaagt ccgattcgcg tttccgcagt acggccggga tgttgcccag
     5701 gttttaaccg gacatgattg ccctcgactt ccggtgcact aatgaccgga atcatcacgg
     5761 ctatcgcccc gtcattccga tggaaacgga tcgagtatcc agagaacgga catattcgca
     5821 taactgcggt agggggcgtg cgccggccca accgcggtgg cagccgcgcc ccgcccggtg
     5881 cacggtaggg aggtggatcc cgtggcggcg ctggagcgga tcgcgttcct gctggagcgg
     5941 cagggggcgg tgacgtaccg ggtgcaggcg ttccgcacgg cggcgtcggt gctcgacgga
     6001 ctggccgcgg aggaactgcg cgagcgggcc gggaacggga ccctggggcg tctgaagggg
     6061 ctcggcccca agacgacccg ggtcgtcgag gaggcactgg ccgggcggca gcccgcctat
     6121 ctgacgcggc tgacggaaga ggccggcggc ccgctggtcg agggggaacg gggactggcg
     6181 ctgcggcggg ccctgcgcgg cgactgccat ctgcactcga actggtcgga cggcggcagc
     6241 cccgtcgagg cgatggccag ggccgcccgc gacctcggcc acgactggtg cgcgctgacc
     6301 gaccactccc cccggctcac cgtcgcccac gggctgaccg ccgagcggtt gcggcggcag
     6361 ctggacgtgg tcgccgaggt caacgcgcgc ctggcgccgt tccgactgct gaccgggatc
     6421 gagtgcgaca tcctcgacga cggctcgctg gaccaggagc cggagctgct ggaccggctc
     6481 gacgtcgtgg tcgcctcggt ccactccaaa ctgcggatgg accggcgacc gatgacccgg
     6541 cggatgctgg ccgcggtcgg caatccgctg gtggacgtgc tcggccactg caccggccga
     6601 cagctcaccg gccggctccg tccggagtcg gcgttcgacg ccgcggaggt cttcgcggcc
     6661 tgcgccgagc accgcaccgc ggtggagatc aactgccgcc cggaccgctt cgacccgccg
     6721 cgccgcctgc tgcggcaggc cctggacgcc ggcaccctct tcgcggtgga cagcgacgcg
     6781 cacgcgcccg gccagctgga ctggcagatc tacggctgcg cccgcgccga ggagtgcggc
     6841 gtccccgccg accggatcat caccacctgg acggcccgcc agctgacgac ctggacgcgg
     6901 accggccgca ccccgcacgg ggccggcggc tgaccgtccg gggccggtcc gggaccactc
     6961 gcctcagccc tgcgcggacg ccgccgtgcg cagcgccgcc aacaccgggc ggaacagcgg
     7021 atggtcctcc gcgccgcggc ggaccgcggc gaagacccgg cgggtgggcg gggcgccgtc
     7081 gaccggtcgc accaccacac cccccaggtc catcccgcgc agcgccgagc gcggcaccag
     7141 ggccaccccc acgcccgccg aggccagcgc caccaccgcc cggaagtcgt ccgaggagtg
     7201 cgtcaggcgc ggccggaagc cggcgtactc gcaggccagc acgaccacgt catggcaggg
     7261 attgccggaa tagggcccga tccaggggtc cttggcgagc ccggccagcg gcaccgccgc
     7321 cgccccggcc agtggatgcc cgggcggcag taccgcgtcg aacggctcgg cgtagagcgg
     7381 gacccgggtc agccgggggt cgtccgcgtc cggcgcgccc cggtactcga cggccaccgc
     7441 cacgtcgacc tgccggtcca gcaccatcag caggctggcg tcgccctccg cgtcctggac
     7501 ccggacccgg atgccgggtg cggaaccggc gagttcggcc agggccgggg cgagcaccag
     7561 cgcgatgccg gtcgcgaacg ccgcgacggt caccgtgccg gcctccccct tgtcgtacgc
     7621 cgccagctcc gcctcggccc gctccagctg ggccagcacg gcgttggcat gaccgagcag
     7681 gatgtcgccc gcggcggtca gccggacgcc gcgggcgccg cgctcgacga ggcggtggcc
     7741 ggtctcctgc tccagggcgg ccagttgctg ggagaccgcc gagggcgtga ggtagagcgc
     7801 ggcggccgcc gccgtcaccg tgcgatggtc ggccagcgca cgcaggatgt gcagccgacg
     7861 ggcttcgatc atggttccag tttcgcaggg ttcggcgggg ttccgtgccc cggaaccccg
     7921 cggtcagccc gccagggcgg cgcgggcggc cacgaaggcg tccaccgcgc ggtgcacgtc
     7981 ctcggtggag tgggccgcgg acagctggac gcggatccgg gcctctccct tggggaccac
     8041 cgggtacgag aagccgatga cgtacacccc gcgctccagc agcagctcgg ccatccgggc
     8101 cgcctcggcc gcgtcgccga tcatcaccgg ggcgatcggg tgctcgccgg gcaggatcgc
     8161 aaagccctcc tcggtcatcc gggagcggaa cagcgcggtg ttggcgttga ggcgttcgcg
     8221 caggtcaccg gcggactcca ggaggtcgag caccttcagc gaggcggccg cgatcaccgg
     8281 cgcgagcgag ttggagaaga ggtaggggcg ggagcgctgg cggagcaggg cgacgatctc
     8341 ggcgcgcgcc gcgacatagc cgccggaggc gccgcccagc gccttgccga gggtgccggt
     8401 gatgatgtcg acgcggtcca tcacgccgca cagctcgggg gtgccgcgac cgccgggacc
     8461 gacgaagccg accgcgtgcg agtcgtcgac catgaccatc gcgccgtagc ggtcggccag
     8521 gtcgcagatc tcccgcagcg gcgcgaggta gccgtccatc gagaacacgc cgtcggtgac
     8581 gatgagggtg cgccgggcgc cctcggcgtc cttcaactgc cgctccaggt cgtccatgtc
     8641 gcggttggcg taacgcagtc ggcgggcctt ggagagccgg atgccgtcga tgatggaggc
     8701 gtggttgagg gcgtcggaga tgaccgcgtc ctccgggccg agcagggtct cgaagacacc
     8761 gccgttggcg tcgaagcagg aggagtagag gatggtgtcc tcctggccga ggaagccgga
     8821 gatccgcttc tccagctcct tgtgcacctc ctgggtgccg cagatgaagc ggaccgaggc
     8881 caggccgtag ccccagcggt ccagggcacc gtgggcggcg gcgatgacct cggggtggtc
     8941 ggccaggccc aggtagttgt tggcgcagaa gttgaggacc tcgccggggc ggccgccggc
     9001 cgtgaccgcc acggtggcgg actgcggggt gccgatgacc cgctcgggct tgtgcagtcc
     9061 ggcggcgcgg atctcgtcga gggcggtgcg cagatcgtcg cgtacggcgt cgaacatgag
     9121 gtggtgtctc cttaggcggg gtcgaggtcg ggcgacggga gggcaggccg gctggcggcg
     9181 ccgtcatcgg gggtggcggg gctcaggcgc tccagtcgag gatgaccttg ccgccctggc
     9241 cgctggccgc gtcggcgaag gcggtctcga actcctggta cgggtagcgg ccggtgatca
     9301 cgggggcgag gtcgaggccg ccttccagca gcaccgacat cgcgtaccag gtctcgaaca
     9361 tctcccggcc gtagatgccc ttgatcgtga tcatggaggt gacgatccgg gaccagtcga
     9421 ccgggaactc ctcggcggcc aggcccagca cggcgatccg gccgccgtgc gtcatgttgc
     9481 cgatcatgtc gcgcatggcc tcgggccggc cggacatctc caggccgacg tcgaagccct
     9541 cgcgcaggcc cagttcgcgc tggccggcct cgatcgaggt gtgggcgacg ttgagggcga
     9601 ggctgacgcc gaccttgcgg gccagttcca ggcgctgctc gctgacgtcg gtgatcacca
     9661 cgtggcgggc gccggcgtgc cgggcgaccg ccgccgccat gatgccgatc ggtccggcgc
     9721 cggtgatcag gacgtcctcg ccgaccagcg ggaaggagag cgcggtgtgc acggcgttgc
     9781 cgaacggatc gaagaccgcg gcgatgtcga ggtcgaccgg gacacggtgc acccacacgt
     9841 tggtggcggg cagcgcgacg tactcggcga aggcgccgtc ccggccgacg cccaggccga
     9901 cggtggcgcg gcacaggtgg cggcggccgg cctggcagtt gcggcagctg ccgcagacca
     9961 ggtggccctc gccgctgacc aggtcgccgg gcttgatgtc ggccacgtcc cggccggtct
    10021 cgacgacctc cccgacgaac tcgtggccga cggtcagcgg ggtccgcacg gcctgctgcg
    10081 cccagccgtc ccaggcccgg atgtgcaggt cggtgccgca gatgccggtg cgcagcacct
    10141 tgatcagcac atcgccgggg ccgatcgtcg gctccggcac gtccgtcagc cagagtcccg
    10201 gttccgcgtt ctgctt
//
