LOCUS       NZ_CP113950            10846 bp    DNA     circular CON 28-JAN-2024
DEFINITION  Myxococcus sp. NMCA1 chromosome, complete genome.
ACCESSION   NZ_CP113950
VERSION     NZ_CP113950.1
KEYWORDS    RefSeq.
SOURCE      Myxococcus sp. NMCA1
  ORGANISM  Myxococcus sp. NMCA1
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1  (bases 1 to 9502182)
  AUTHORS   Ahearne,A., Stevens,C. and Phillips,K.
  TITLE     Minimal conservation of predation-associated metabolite biosynthetic
            gene clusters underscores biosynthetic potential of Myxococcota
            including descriptions for ten novel species: Archangium lansinium
            sp. nov., Myxococcus landrumus sp. nov., Nannocystis bai
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9502182)
  AUTHORS   Ahearne,A., Stevens,C. and Phillips,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2022) Biomolecular sciences, University of
            Mississippi, Faser Hall 407, Oxford, MS 38655, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 10.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 03:49:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,878
            CDSs (total)                      :: 7,793
            Genes (coding)                    :: 7,662
            CDSs (with protein)               :: 7,662
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 56 of 131
            Pseudo Genes (incomplete)         :: 88 of 131
            Pseudo Genes (internal stop)      :: 13 of 131
            Pseudo Genes (multiple problems)  :: 24 of 131
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:28:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6380458
            Orig. end                         :: 6391304
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113950.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10846
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10846
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10846
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5846](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5846)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            329..1453
                     /locus_tag="OZ403_RS26175"
                     /old_locus_tag="OZ403_26175"
     CDS             329..1453
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554930.1"
                     /locus_tag="OZ403_RS26175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_26175"
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="WP_268782062.1"
                     /transl_table=11
                     /translation="MRSTFHIAVATLLACCAAGCGNLENAPFRVGTVHGQLTESDPSVA
                     MVSLVDQPGVSSHVDADGRFTLEDIPTGMAELFIVATAEKAARVQVQVLGGQSVQVQPV
                     APTPAGFLDLRVKTTNGFRLSAAEVSVKGTPFQRLLLDAKGRLRVGPLPDGCYSVTVTA
                     LGFPATQVEDCAGPGEKKELKVDLEADESLLEQGCQEIGCEEGLVCAPNKKCLECFGNS
                     QCGAGLTCKGNRCEGPGPLCAPCTGDWQCAAGTHCEVLPEGSAACVARCGDDDDAPPTA
                     QALSVDDYSARCAPGFTCQSGRCLPDAANFAGCHALRRMDAPCTDDANCHELGLPGGRC
                     VSGACTVPCATDRDCPDTRWCVTSSAGRVCQVGT"
     gene            1458..4247
                     /locus_tag="OZ403_RS26180"
                     /old_locus_tag="OZ403_26180"
     CDS             1458..4247
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554931.1"
                     /locus_tag="OZ403_RS26180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_26180"
                     /product="hypothetical protein"
                     /protein_id="WP_268782063.1"
                     /transl_table=11
                     /translation="MASQGTPRLRIILVTLYTVTTWGCEPAPRHPSTVHNTPPRLEVSP
                     TLDITPLRVGQAVEFVASVEDAEDGAALGANVLWVSSVEGQLARGARATLAFSEPGEQR
                     LTASVVDAGGLAASASLHLNVLARGAPVATVLRPAPGSTFNLGEVLGLECVAMTERGAR
                     LQGRAVQWTSALSGPLPAGDVVRATLSVAGEDSLRCTAVDSATGVSATAAVRVTVRATR
                     APSVRILRPEPAEIFVKQGEAPPHGASVRFAATAQDFHAEGGAGNLDGVVEWRLAPTGR
                     PLGRGPALTHAFTAPGVYTVMARVVDGRGNTASDSVQVRLVTNLPPRCELELPATENPR
                     LLVGVPSRLRARCVDPETEQPVEPTWTTSAEPTPLGTGEAVSAVLSVAGAQVLSACAVD
                     PEDTELRGCVERPVRAIVNTPPIGCAIQAPLPDAVVNAGVPLTLTGSASDAEDARTGLR
                     YTWVSSRDGALAQGPSTHTVRLSTPGHHVLTLTATDPWGLACAATVAVTVNGAPDVRVE
                     SVLQGVLDCGRGPCLEGQPVFLTGFARPAAPQGHIAELTWLDNLAGSLLAVALPLPTAT
                     LALPLAGRHTTVLAATDQNGAVSRAATSFTVLPLGGARLMEDVPDAQRPVVALALTPEG
                     LRYADGASPRVYAERASTTVLDVDAPALSLHTFQGGDGNVLFVGTDGGGVLRCVQHTCT
                     RHTGAMLGATGAAVTAVAAWESPDVLLLGTRRGVVLTRASDPASGGRMGTVGGRRLLDG
                     REVRQLAISPDSSPERVTAWAATSGGLAEITILADDDFEPGLAEVVVRIHTPPAIPDDD
                     VLSVAVGPEGQVFAGTRKGFSALGQPGPALRDAPWGLQDEEIRALHFERQTVGARTRDI
                     LWAGTKNGLVRYDVSVDIATHFGTEEGLPDPDVSALLVAPGGPRYVGTARGLVTYPWH"
     gene            complement(4270..5004)
                     /locus_tag="OZ403_RS26185"
                     /old_locus_tag="OZ403_26185"
     CDS             complement(4270..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941507.1"
                     /locus_tag="OZ403_RS26185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_26185"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_140856017.1"
                     /transl_table=11
                     /translation="MKPSLRDFFDSMGSYLAEPGPESLAALYARHPGWEAPRERVSLYG
                     GFVLGHVRSALDKVFPLTRQALGQDAWDGLVAAFTRKRPARHHELNHLCEGFPAFLADA
                     ASRGLALFVPALARFEWTDFAVFASLEPSPERVERLTANPTLVVLEHDFHLCAYVRARG
                     VATSPASGAELALLWRHPEQLKTLYMAATPPALLVLKMAVEGLALADVVAATGMAEADL
                     RATVARFGRDGLVLLPGDGSGG"
     gene            complement(5001..5846)
                     /locus_tag="OZ403_RS26190"
                     /old_locus_tag="OZ403_26190"
     CDS             complement(5001..5846)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350979.1"
                     /locus_tag="OZ403_RS26190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_26190"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_140856019.1"
                     /sec_met_domain="DUF692 (E-value: 5.4e-94, bitscore: 313.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTYAQRHGLKPLGAGIGLRRSFYAELPRTGRTLDWVELIPENFL
                     SLGGRSQRALDACAERWPVLPHGVGLNIGGPDALDDDYVRGLSALVKRVDAPFFSDHLC
                     FSRLDGVYLHDLLPLPFSEAAVEHVVPRVREVMDRVGRPFLLENPSYYARMPGGTLREA
                     DFLRHVVEQADCGLLLDVNNVYVNARNHGYDARAFVDALPLERVVQIHLAGHTCYPDVI
                     VDTHGDRVCDDVWSLYRYVLARTGPVATLIEWDQDIPSLDAVLDEADVARAALREVAGR
                     "
     gene            complement(5961..6206)
                     /locus_tag="OZ403_RS26195"
                     /old_locus_tag="OZ403_26195"
     CDS             complement(5961..6206)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941509.1"
                     /locus_tag="OZ403_RS26195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_26195"
                     /product="hypothetical protein"
                     /protein_id="WP_140794029.1"
                     /transl_table=11
                     /translation="MNVKALAAVVGTLSLAALATGCVTKKSAEMEPATAEETGAESSGA
                     TESQEAAPAATEEKGAEGSCGAGSCGSGSCGGQKKE"
     gene            complement(6265..7680)
                     /locus_tag="OZ403_RS26200"
                     /old_locus_tag="OZ403_26200"
     CDS             complement(6265..7680)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637554.1"
                     /locus_tag="OZ403_RS26200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_26200"
                     /product="hypothetical protein"
                     /protein_id="WP_268782064.1"
                     /transl_table=11
                     /translation="MRARAAAVILFGLSLMSCQAPLRHHPREDAAALRYNLTYVREPEH
                     AVDVEVVLVRGTPREFLFSQPGAVTTVSVHREDGGVQTLPVREGRVLLPRGTRFLRYRY
                     PLDASIRERGPDFFQGSGDGDARHVAGRAYLIRPRVVKPGLRVELSLVGTDALLPWQPG
                     ESGVYRLRGEDLVDSGFHGFGGRRCQVRLPDAVLDVAVLGHFTHLSDAAVCTWLGKAAE
                     EVRTVRRAFPHPRITVRVIPAPGHRGPSVFGMVRWSSPPSISISVGQEARPEDFARDWV
                     AVHEMLHLTHPVFVPRVPWLSEGLATYYTEVARARSGRQDSRRAWEELLGGFARGREGN
                     DTRTMQEAVSQDSFSLAVYWTGALFALHLDVELRRITGNTRGLDDVLELLAARGSSSTF
                     GAFGAAVDAVAGQPLFNALLERHLPRPAFAEQAGLLEALGVQDGPNGVSLVPARDSLLR
                     DSVVGRRSHGETP"
     gene            <7992..8363
                     /locus_tag="OZ403_RS26205"
                     /old_locus_tag="OZ403_26205"
                     /pseudo=""
     CDS             <7992..8363
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2fas"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621051.1"
                     /locus_tag="OZ403_RS26205"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_26205"
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase II"
                     /pseudo=""
                     /sec_met_domain="t2fas (E-value: 1.6e-42, bitscore: 144.6,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GYINAPGTSTDLGDALEMEGIARVFGDAARSVAVSSTKSMTGHMN
                     GAAGTAEAIISVLALTRGLLPPTINLERKDPRIQLDCVPHTARPAQVDAVTSNSFGFGS
                     TNVSLVFRRRSPTASSASL"
     misc_feature    8415..8431
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(8568..8583)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.56"
                     /tool="antismash"
     misc_feature    complement(8581..8595)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.95"
                     /tool="antismash"
     misc_feature    8594..8608
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.15"
                     /tool="antismash"
     misc_feature    complement(8602..8614)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.58"
                     /tool="antismash"
     gene            8674..10293
                     /locus_tag="OZ403_RS26210"
                     /old_locus_tag="OZ403_26210"
     CDS             8674..10293
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554936.1"
                     /locus_tag="OZ403_RS26210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_26210"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_140794031.1"
                     /transl_table=11
                     /translation="MNDNALNANVGLKLRGLRLARNIKQADAAKDLGVSPAYLNLIEKG
                     KRVMPFPLLWKALRYFDQDPEQFMSTLGEGRVDEALAKLLDEPLLKSLDIDPESLQSLS
                     AEPKLAGTVAALFNLYKNTRTQLENVLAQLNVEERTRAQGGLGNGAAPGVRFDYSPFDE
                     VSDFLEKHRNYFPELEEQADALRRDVRLERQLTSGQLIRLLEERFGYRVLFDDPSPSGS
                     SVVRRLDPEERTLTLSPFLTEQPLKFQIAASIGLLVMDREKLLERVLDAGRMRHGETQR
                     LIKVNLANYFAGALMLPYGDFFKEVQRTRYDVELLSSIFGSTYETVAHRLCNLSDPKRP
                     GLPFHFLRADIAGNISKRYSGTGIRFATGGGSCAKWAVHLAFLNPSQLTRQYSIMPDGT
                     AYFCFAKVQLQPIEGSIVKGTAYSIGLGTHAENAKYLAYGLPTTDLRKDAIPSGISCRF
                     CERTDCNQRAAASYRFAFAFDEYTKKDCFFSPILVHEKADKVEKDRHPGAHSLAVAANG
                     TDGSEKDDSLDKTSRRRKGGDA"
     misc_feature    10740..10756
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.32"
                     /tool="antismash"
ORIGIN
        1 cgggcagcga ggtgcccttc cgctacacca ccgtctccgc cggagcggcc cttccctacc
       61 tgtggcgctg ggagcggctg acgttgttcg cggggccgcg tgtggccggc ctgtacctca
      121 ggcggtcctt tgacgtagag gctgccgcca acgggcaacg gttcttcacc gtcagccccg
      181 gcgtggtggg cggcatcgtg tggcgggtgg gtgagcggct ggagctgatg tcacaggccc
      241 agttcatgct gacgtatgtc gttgtggatg ggcaggggca ggccgtgggt ttcacgggtg
      301 gctgggccgg cgcgggatat cgtttctgat gcgctcgacg tttcacatcg cggtagccac
      361 cctcctggcc tgctgcgccg cgggctgcgg caacctggag aacgccccct tccgggtggg
      421 caccgtgcat gggcagctca cggagagcga cccctcggtg gccatggtgt cgctggtgga
      481 ccaacccggc gtgagcagcc atgtcgacgc ggacgggcgc ttcaccctgg aggacatccc
      541 cacggggatg gcggagctct tcatcgtcgc cacggccgaa aaggccgccc gcgtccaggt
      601 gcaggtgctg ggaggccagt ccgttcaggt acaaccggtg gcgcccacgc ccgcgggctt
      661 cctcgacctg cgcgtgaaga cgaccaacgg cttccggctg tccgcggcgg aggtgtccgt
      721 gaagggcaca cccttccagc ggctgctgct ggacgcgaag gggcggctgc gcgtggggcc
      781 ccttccggac ggctgctact ccgtgacggt gacggccctg ggctttccgg ccacccaggt
      841 cgaggactgc gcgggccccg gcgagaagaa ggagctcaag gtcgacctgg aggcggacga
      901 gtcgctcctc gaacagggct gccaagagat tggctgcgag gaaggcctgg tgtgcgcgcc
      961 gaacaagaag tgcctggagt gcttcggcaa ctcgcaatgc ggcgcgggcc tgacgtgcaa
     1021 gggcaaccgc tgcgagggcc ccggtcccct gtgcgcgccc tgcacgggcg actggcaatg
     1081 cgccgctggg acgcactgcg aggtgctgcc cgaaggaagc gccgcgtgcg tcgcccggtg
     1141 tggcgacgac gatgacgccc cccccacggc ccaggccttg tcggtcgacg attactcggc
     1201 gcggtgcgcg ccgggcttca cctgccagtc cgggcggtgc ttgccagatg ccgcgaactt
     1261 cgcgggctgc catgccctgc ggcggatgga cgcgccctgc accgacgacg cgaactgcca
     1321 cgagctggga ctgcccggcg ggcgctgtgt cagcggggcc tgcacggtgc cctgcgcaac
     1381 ggaccgtgac tgcccggaca cccggtggtg cgtgacttca tcggcgggcc gcgtctgcca
     1441 ggtcgggacg taacaccgtg gcgagccaag gcaccccgcg gctccgcatc atcctggtca
     1501 ccctgtacac cgtcacgacg tggggctgtg agccggcccc ccgccatcct tcgacggttc
     1561 acaacacccc tccccggctc gaagtcagcc ccaccctgga catcaccccg ctgcgagtgg
     1621 gccaggccgt ggagttcgtg gcctccgtcg aggatgcgga ggacggcgcc gcgctgggcg
     1681 ccaacgtgct gtgggtgtcc tccgtcgaag ggcaactggc ccgcggcgca cgcgccaccc
     1741 tcgccttcag cgaaccggga gagcagcggc tgaccgcctc ggtggtggac gccggtggac
     1801 tggccgcgag cgcgtcactc cacttgaacg tcctcgcgcg gggcgctccc gtggccaccg
     1861 tgctgcgccc cgccccgggc agcaccttca acctcggcga ggtgttgggc ctggagtgcg
     1921 tggcgatgac ggagcgtggc gcgcggctcc aggggcgcgc ggtgcagtgg acgtccgccc
     1981 tgtccgggcc gctgcctgcc ggcgacgtgg tgcgggcaac cctgtccgtg gccggcgagg
     2041 acagcctccg ctgcacggcg gtggactccg ccacgggtgt ctccgccacc gccgcagtgc
     2101 gcgtcaccgt ccgcgccacg cgcgctccca gcgtgcgcat cctccggccc gagcccgcgg
     2161 aaatcttcgt gaagcaaggc gaggcaccac cccacggcgc ctccgtccgc ttcgcggcca
     2221 ccgcccagga cttccacgcc gagggcggcg cgggcaacct ggacggcgtg gtggagtggc
     2281 gcttggcgcc cactgggcgt ccgctgggcc gaggcccagc cctgacacac gccttcaccg
     2341 cgcccggcgt ctacacggtg atggcgcggg tggtggatgg gcgcggcaac accgcctcgg
     2401 acagcgtgca ggtgcggctg gtcaccaacc ttccgccccg gtgcgagctg gagctccccg
     2461 cgaccgagaa tccacgcctg ctcgtgggcg tccccagccg gctgcgggcc cgctgcgtgg
     2521 acccggagac ggagcaaccg gtggagccca cctggaccac cagcgccgag cccacgcccc
     2581 tgggcacggg tgaagccgtg agtgcagtgc tgtccgtggc cggtgcgcag gtgttgtcgg
     2641 cgtgcgccgt ggaccccgag gacaccgagc tgcgcggctg tgtggaacgg ccggtgcgcg
     2701 ccatcgtcaa cacgcccccc attggctgcg ccatccaggc accgcttccg gacgcggtgg
     2761 tgaacgcggg tgtgccgctg acgctcacgg gttcggcgtc ggacgcggag gatgcgcgga
     2821 cgggcctgcg ctacacgtgg gtgtcctcgc gggacggcgc cctggcccag ggcccgagca
     2881 cgcacaccgt ccggctgtcc acgcccggcc accacgtgct gacgctcacc gcgacggacc
     2941 cctggggact ggcctgcgcg gcgaccgtcg ccgtcactgt gaatggcgcg cccgacgtgc
     3001 gggtggagag tgtgctgcaa ggcgtgctgg attgcggcag aggcccatgc ctggaggggc
     3061 agcccgtgtt cctcaccggc ttcgcgaggc ccgcggcccc tcaggggcac atcgcggagc
     3121 tgacgtggct ggacaacctg gcggggagtc ttttggcggt cgcgctgccg ctgcccaccg
     3181 ccaccctcgc cttgcccctc gccggtcgcc acacgacggt gctggccgcc accgaccaga
     3241 acggcgcggt gagccgcgcg gcaacgtcct tcacggtgct gccgctggga ggcgcgcggc
     3301 tgatggagga cgtgccggac gcgcagcggc ccgtggtggc cctggcactc accccggaag
     3361 ggctccgcta cgccgacggc gcatcccctc gggtgtatgc ggagcgcgcc tccacgacgg
     3421 tgctggacgt ggacgccccg gccctgtcgc tccatacgtt tcaaggagga gacggcaacg
     3481 tgctcttcgt gggcaccgac ggcggcggcg tgctccggtg cgtccagcac acctgcacgc
     3541 gacacacggg cgccatgctg ggcgccacgg gcgcggcggt gaccgcggtg gccgcgtggg
     3601 aatccccgga cgtgctgctg ctgggcaccc gccggggcgt ggtcctcaca cgcgcgagcg
     3661 accccgcctc cggaggacgc atgggcaccg tgggcggccg ccgcctgctg gacggacgcg
     3721 aggtgcgcca gctcgccatc tccccggact ccagcccgga acgcgtgacg gcctgggcgg
     3781 ccacctcggg tgggctcgcg gaaatcacca tcttggcgga cgacgacttc gagcccgggc
     3841 tcgccgaagt cgtggtgcgg atccacactc caccggccat tcccgatgac gacgtcctgt
     3901 ccgtggccgt ggggcccgag ggccaggtgt tcgccggcac gaggaagggc ttcagcgcgc
     3961 tgggacagcc cggtcccgcg ctgcgcgacg cgccctgggg actgcaggac gaggagattc
     4021 gcgcgctcca cttcgaacgg cagaccgtgg gcgcgcgcac ccgggacatc ctctgggccg
     4081 gcacgaagaa cgggctggtt cgctatgacg tgtcagtaga catcgccacc cacttcggaa
     4141 cggaagaggg cttgccggac ccggacgtga gcgccctgct cgtggcgccc ggaggaccgc
     4201 gctacgtggg cacggcgcga ggcctggtga cgtacccctg gcactgagcg cccggacagc
     4261 gcgggccctt caacccccgc tgccgtcacc cgggaggagc accaatccat cgcggccgaa
     4321 gcgcgccact gtcgctcgca ggtcggcctc cgccatgccc gtggccgcca ccacgtccgc
     4381 cagtgccaac ccctccaccg ccatcttcag caccaacagc gccggcggcg tggcggccat
     4441 gtagagcgtc ttcaactgct ccgggtggcg ccacagcagc gccagctccg cgcccgacgc
     4501 tggtgacgtc gcgacgcctc gggcccgcac gtacgcgcac aggtggaagt cgtgctccag
     4561 caccaccagg gtgggattgg ccgtcagccg ctccacgcgt tcgggcgaag gctccaggga
     4621 cgcgaacacc gcgaagtcgg tccactcgaa gcgggccagt gcgggcacga acagcgccaa
     4681 tccccgcgag gccgcgtccg cgagaaacgc gggaaagcct tcgcacaggt ggttgagctc
     4741 atggtgccgc gcgggccgct tccgcgtgaa ggccgccacc aggccgtccc atgcgtcctg
     4801 ccccagggcc tggcgcgtca gcggaaacac cttgtccagc gcggagcgca catggccgag
     4861 cacgaagccg ccgtagagag acacccgctc acgcggcgcc tcccaccccg ggtgcctcgc
     4921 gtacagcgcc gccagtgact ccgggccggg ctcggcgagg tagctcccca tgctgtcgaa
     4981 gaagtcgcgc agggacggct tcatcggccg gcgacctccc gcagcgcggc tcgcgccaca
     5041 tccgcctcgt ccagcaccgc gtccagggaa ggaatgtcct ggtcccactc gatgagcgtc
     5101 gctaccggac cggtgcgcgc cagcacgtag cggtagagcg accacacgtc gtcgcagacg
     5161 cggtcgccat gcgtgtcgac gatgacgtcc gggtagcacg tgtggccggc caggtgaatc
     5221 tgcaccacgc gctccagcgg cagggcgtcc acgaaggccc gcgcgtcgta gccatggttg
     5281 cgcgcgttga cgtagacgtt gttcacgtcc agcagcagcc cacagtccgc ctgctccacg
     5341 acgtggcgga ggaaatccgc ctcccgcagc gtgccgccgg gcatccgcgc gtagtagctc
     5401 gggttctcca ataggaaggg acggcccacg cggtccatca cctcacgcac gcggggcacc
     5461 acgtgctcga cagcggcctc gctgaacggc agcggcagca agtcgtgcag gtacacaccg
     5521 tccagccgcg aaaaacagag gtggtcggag aagaagggcg cgtccacccg cttcaccagc
     5581 gccgacagtc cccggacata gtcgtcatcc agcgcgtccg ggccgccgat gttcaacccc
     5641 acgccatggg gcaacacggg ccaccgctcc gcgcatgcgt ccagcgcgcg ctgcgagcgc
     5701 ccccccagcg acaggaagtt ctccgggatg agctccaccc agtcgagcgt ccgtccggtg
     5761 cgcggcagct ccgcatagaa gctcctgcgc agcccgatgc ccgcacccag cggcttcagc
     5821 ccatgtcgct gtgcgtacgt caccacggca gaagccctct cgaggcgtgg gaaacaaccc
     5881 gggcggcggt ggagctgctc aaccgcccgg caacaagggc ccggccttca ccaggccctc
     5941 gggacttcgg tccggaggaa ctactccttc ttctggccgc cgcaggagcc ggagccgcac
     6001 gagccggcgc cacagctgcc ctcggcgccc ttctcctcgg tcgcggccgg agcggcctcc
     6061 tgcgactccg tggctccgct gctctccgcg ccagtctcct cggccgtggc gggctccatc
     6121 tccgccgact tcttggtcac acaacccgtg gccagcgcgg ccagggacag ggtgccgaca
     6181 accgctgcaa gcgccttgac gttcatggat gagacttcct ttcggtactg cgtggggggt
     6241 gttgtgtgga ggtcacgggt gcattcaggg tgtttcgccg tggctgcgcc gaccgaccac
     6301 cgaatcacgc aacagactgt ctcgggcggg taccagcgag acgccgttgg gtccgtcctg
     6361 gacacccagc gcttctagca gaccggcctg ttccgcaaaa gcaggccgcg gcaagtgccg
     6421 ctccagcaga gcattgaaca gcggctgtcc cgcgacggca tccaccgcgg caccgaatgc
     6481 cccgaaggtc gaggaactgc cgcgagccgc gagcaactcc agcacgtcat ccagcccgcg
     6541 cgtgtttcca gtgatacggc gcaattccac atccagatgc aacgcgaaga gcgctcccgt
     6601 ccaatagaca gcgagcgaaa agctgtcttg agacacggcc tcctgcatgg ttcgcgtgtc
     6661 attgccctct cgcccccgag cgaagccgcc cagcagttcc tcccaggcgc gccgcgagtc
     6721 ctgccgcccg gagcgcgcac gcgcgacctc cgtgtagtag gtggccagcc cctccgacag
     6781 ccacggcacg cggggcacga agacaggatg ggtcaggtgc agcatctcgt ggacggccac
     6841 ccagtcccgc gcgaagtcct ccggccgcgc ctcctgcccc acgctgatgg agatgctggg
     6901 cggcgagctc cagcgcacca tgccgaagac gctgggcccg cggtgcccag gcgcggggat
     6961 gacacgcacg gtgatgcgcg gatgcgggaa ggcgcgccgc acggtgcgga cttcctccgc
     7021 ggccttgccc aaccaggtgc agacggccgc gtcgctcagg tgcgtgaagt gtccgaggac
     7081 ggccacgtcc agcaccgcgt ccggcaggcg cacctgacac cggcggcctc cgaagccgtg
     7141 gaagcccgag tccaccaggt cctcgccgcg cagccggtac acgccactct ccccgggctg
     7201 ccacggcagc aacgcatcgg tgcccaccag cgacagctcc acgcgcaggc ccggcttcac
     7261 cacccgcggc cggatgaggt acgcgcggcc cgcgacgtgc cgcgcatcgc cgtctccgga
     7321 gccctggaag aagtcagggc cccgctcccg gatggaggca tccagcgggt agcggtagcg
     7381 caagaagcgc gtcccccgag gcagcaagac gcggccctcc cggacgggca acgtctggac
     7441 gcccccatcc tcccggtgca cggacaccgt cgtcacggcg cccggctgac tgaagaggaa
     7501 ctctcgcggc gttccccgca ccagcaccac ctccacatcc accgcgtgct cgggctcacg
     7561 cacgtaggtg aggttgtagc gcagcgccgc cgcatcctcc ctggggtggt ggcgcagtgg
     7621 cgcctgacac gacatcagcg acaggccgaa caggatgacg gcggcggctc gggcacgcat
     7681 gcgcagcggt gccctgctcc acgttcgcgg ccatccatgc ccaccgtcca tacacggtga
     7741 aggttcgccc atgacgcgcc ggaggtgaat ggcgatgagg cctccgggag cgcgggccga
     7801 cacttctcca gcgtcaaccc acactccgtg gcacggcggg gcagcccgag cgtcgtgctc
     7861 agcggggctt cttccgggcg ggagccgcgg ccgggtcacc cggaccagcg gcatggcccc
     7921 gaaacgaagc gccagcgtcg ccagcgcgcg cttgcgcgcc gtgacaccgc cctcccctgg
     7981 cacacgggag cggctacatc aacgcccctg gcacatcgac ggacctgggc gacgccctgg
     8041 agatggaggg cattgcccgc gtcttcgggg acgcggcgcg ctcggtcgcc gtctcttcga
     8101 ccaagtcgat gacgggccac atgaacggcg cggccggcac cgcggaggcc atcatcagcg
     8161 tgctggccct cacccgtggc ctgctgccgc ccaccatcaa cctggagcgg aaggacccgc
     8221 gcatccagct ggactgcgtg ccccacacgg cccgccccgc gcaggtggac gccgtcacga
     8281 gcaactcgtt cggcttcgga agcaccaatg tgtccctggt gttccggcgc cgatcgccca
     8341 cggcctcctc cgccagcctg taaacccacg ctttcgcgtt taccagaacg tgccccaccc
     8401 atgctgcaat gcatcagtaa atgtttgaca aggcgctttg gagagagagg cggcaggttc
     8461 cccacacgcc ctgaagggca gtgatttcat ggacttgaat gtacccggcg ccctggttcc
     8521 gaccgccggc cagccggtcc gtcgccgggc cgacaggggt cctcgtggat gaaaacgatc
     8581 attgcaaatc ggtaaatcga tttgcaaagt gggtgaagag cccgaggcgc gcgcggccat
     8641 tcgcgatcgc cgggggcatc aacgcatccg gccatgaacg acaacgcact gaacgccaac
     8701 gtgggcctga agcttcgggg tctgcgccta gcacggaaca tcaaacaggc ggacgcagcc
     8761 aaggacctgg gcgtttcccc cgcgtacctg aacctcatcg agaagggcaa gcgggtgatg
     8821 cccttccccc tgctctggaa ggccctgcgc tacttcgacc aggaccccga gcagttcatg
     8881 tccacgctgg gcgagggccg cgtggacgaa gcgctcgcga agctgctgga cgagccgctc
     8941 ctcaagagcc tcgacatcga cccggagtcg ctccagtccc tctccgcgga gccgaagctc
     9001 gcgggcacgg tggcggcgct cttcaacctc tacaagaaca cccgcacgca gttggagaac
     9061 gtgctggcgc agctcaacgt cgaggagcgc acgcgcgccc agggcggact gggcaacggc
     9121 gccgcgcccg gcgtgcgctt cgactactcg cccttcgacg aggtcagcga cttcctggag
     9181 aagcaccgca actacttccc ggagctggag gagcaggcgg acgcgctgcg ccgcgacgtg
     9241 cgcctggagc ggcagctcac cagcggtcag ctcatccggc tcctggagga gcgcttcggc
     9301 taccgcgtcc tcttcgacga cccgtctccc tccggctcgt ccgtggtgcg gcggttggac
     9361 ccggaggagc ggacgctgac gctgtcgccc ttcctcaccg aacagccgct gaagttccag
     9421 attgccgcgt ccattggcct gctggtgatg gaccgggaga agctgctgga gcgcgtgctg
     9481 gacgcgggcc gcatgcgcca cggcgagacg cagcggctca tcaaggtgaa cctggccaac
     9541 tacttcgccg gcgcgctgat gctgccctac ggggacttct tcaaggaggt ccagcgcacg
     9601 cgctacgacg tggagctgtt gtccagcatc ttcggctcca cctacgagac ggtggcccac
     9661 cgcctgtgca acctgtcgga cccgaagcgc cctgggcttc cgttccactt cctgcgcgcg
     9721 gacatcgccg gcaacatctc caagcgctac agcggcaccg gcatccgctt cgccaccggc
     9781 ggcggcagct gcgccaagtg ggccgtgcac ctggccttcc tcaacccgtc ccagctgacg
     9841 cggcagtact ccatcatgcc ggacggcacc gcctatttct gcttcgccaa ggtgcagctc
     9901 cagccgatcg agggctccat cgtcaagggc accgcctact ccatcggcct gggcacccac
     9961 gcggagaacg ccaagtacct ggcctacggc ctgcccacga cggacctgcg caaggacgcc
    10021 attccgtccg gcatctcctg tcgcttctgc gagcgcaccg actgcaacca gcgcgcggcg
    10081 gccagctacc gtttcgcctt cgccttcgac gagtacacga agaaggactg cttcttctcc
    10141 cccatcctcg tccacgagaa ggccgacaag gtggagaagg accggcaccc gggcgcccat
    10201 tccctggccg tggccgccaa cgggactgac gggagcgaga aggacgattc gttggacaag
    10261 acctcccgcc gccgcaaggg tggcgacgcc tgaggccccc catgcaccac ccgcccgaag
    10321 acacccaacg cacccacatc ctcgacgcca tccagaagca gaagaacgcg ctggcgccgc
    10381 tgcgcatcac cggctcgccc accgaagtgg gccagggcct ggtgaacctg gccgaactgc
    10441 acggcctgct ggaggaccac gccgccagcc ggcagttcta cgaagaggcg ctcgagaagt
    10501 tccaggaggc caagtacaag cccggccagg cgcaggccct gatggggctg ggcgtggtga
    10561 aggcgaactt cgaggatcac cgcggcgcca tcgagcagat tgcccgcgcc gccatgctct
    10621 tcaacgagtc caaggaccgt gagggcgagg ccctggcccg cgcctgcatc ggcgagtccc
    10681 tgcgctcgtt gggccagccg gaagccgccg aggagaagta ccaggaggcg ctcatcctcc
    10741 tgcgccagac gcgcaacacg gagcgggtgg cgcggctgct catcgacatt ggcgacatcc
    10801 gcatggagaa gggcgactac gagcccgcgc gcaagcgctt cctgga
//
