LOCUS       NZ_CP113950            11653 bp    DNA     circular CON 28-JAN-2024
DEFINITION  Myxococcus sp. NMCA1 chromosome, complete genome.
ACCESSION   NZ_CP113950
VERSION     NZ_CP113950.1
KEYWORDS    RefSeq.
SOURCE      Myxococcus sp. NMCA1
  ORGANISM  Myxococcus sp. NMCA1
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1  (bases 1 to 9502182)
  AUTHORS   Ahearne,A., Stevens,C. and Phillips,K.
  TITLE     Minimal conservation of predation-associated metabolite biosynthetic
            gene clusters underscores biosynthetic potential of Myxococcota
            including descriptions for ten novel species: Archangium lansinium
            sp. nov., Myxococcus landrumus sp. nov., Nannocystis bai
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9502182)
  AUTHORS   Ahearne,A., Stevens,C. and Phillips,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2022) Biomolecular sciences, University of
            Mississippi, Faser Hall 407, Oxford, MS 38655, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 10.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 03:49:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,878
            CDSs (total)                      :: 7,793
            Genes (coding)                    :: 7,662
            CDSs (with protein)               :: 7,662
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 56 of 131
            Pseudo Genes (incomplete)         :: 88 of 131
            Pseudo Genes (internal stop)      :: 13 of 131
            Pseudo Genes (multiple problems)  :: 24 of 131
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:28:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5242200
            Orig. end                         :: 5253853
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113950.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11653
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="16"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11653
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11653
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6653](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6653)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    71..82
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            86..2284
                     /locus_tag="OZ403_RS21605"
                     /old_locus_tag="OZ403_21605"
     CDS             86..2284
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480692.1"
                     /locus_tag="OZ403_RS21605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21605"
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="WP_268781614.1"
                     /transl_table=11
                     /translation="MTRIRIGQRWKREPAASPLDSIALELDGVDLLSGAVEESLAEVVP
                     SLVRSVAALAGGRQKLAQVSLPEAHLELVLRRMGPEVELLVANLSRPARLMRPPVRVEL
                     EELVKTVREAGERFLADVTRAGPKSLATTVGQALKEPLKGLNRPARPPDEAPARPATRR
                     VEPTEVPGFGFELRDAGVPMGRGAARGTAGLLAPLLASGEVWLSLPGKAEAWRVPGPPF
                     LTALELSRQAVELARAVELGESRFELAPAGAKPSLLVDLKSGQAKAGRTGTPFPLAGNV
                     LAAALFHLGESLAAAFAEADRALVANPYRMELAERCREGLSHLRGPVQPPEARGAAREK
                     KARATGQGTSRPLKVPGRLRRLRFAKLWEKRGLPDAEESRLLLGRHGPVYCAPQLASAF
                     SRKDGALLWKRAAALGVAASADGHAVAADIARVYGFTGRGGGARWLHDHDGIPLGPLLL
                     RKDGLLLTLSEDRTVVAFAEATGREVWRLAPPRTQRSWLATQGHRALVGTDSGYLYGLD
                     LEDGQVRYRMRAPMPFHGPPVAWGRRFLAMLGRGSHWAVLLADAHTGESVWTHEPDLSH
                     LSAPLPVGSRVFIAGEREREGMLLCLDAKGRRLWERPLNLGPGPYALAPLPRAVVVTSA
                     SGAATRVAASGTVDWRVGAAGEPLISALPAHTARDVTLVPGERVRAVDPRGGQVLAEVQ
                     AGVGLVALQADVRLNLYFLDDAGTLSAYRLGSHFTVVE"
     gene            complement(2298..3212)
                     /locus_tag="OZ403_RS21610"
                     /old_locus_tag="OZ403_21610"
     CDS             complement(2298..3212)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638450.1"
                     /locus_tag="OZ403_RS21610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21610"
                     /product="hypothetical protein"
                     /protein_id="WP_174259314.1"
                     /transl_table=11
                     /translation="MKRYFGVIVIVAGVLLAAVMSYRSASGRALEAQRDADQQRIHAEY
                     LERVGWMRANPDEASYRDELKPFFKHYFEQVDAHLTRFKGNTKFDDYLLELEKREESGA
                     KDDRANDRKAFYEYTRKTFDSLREGRYRPVWTATDKGMRLDIISSDVVMVLGKPQIRLQ
                     LAVWGAQRVLKDDGKVLKMMTSATFDTVWKLTDAKGKLLGEMRGADPSMKIDHPERFVR
                     AFPPQMVLGHYDLDLLPNEVAKVEMTINLSSSAASGGTAASTYVWKLDVPSEWKLGAGE
                     SWEGATQEERPEEEIDPSKAAAN"
     misc_feature    complement(3242..3256)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.0"
                     /tool="antismash"
     misc_feature    complement(3243..3256)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.01"
                     /tool="antismash"
     gene            3264..4295
                     /locus_tag="OZ403_RS21615"
                     /old_locus_tag="OZ403_21615"
     CDS             3264..4295
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554161.1"
                     /locus_tag="OZ403_RS21615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21615"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_140792542.1"
                     /sec_met_domain="PF00561 (E-value: 7.6e-08, bitscore: 31.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQATGTHLFTTPLPLEEGELLGNPQVAWAAYGEPSDGKAVVLLH
                     DLAHSHLALGPPEDSAYQPSGWGRALVGTNLALDPAITPVFSPGLLGSPFGSTSPATVN
                     PASGERWGLELPPLTVQDMARAVSATLRARGLTQVRALVGVGLGAHVALRLASLFPDLA
                     EGVVAIGAARALPEGVREKLGLSWQVLRADPDYRDGLYALDAPPRKTMRKLRLDFLKLL
                     CGREFLASAYPAPDAARAALETEADAFADAFDAVSWASLCSAYAGSDVSDGFSRIRARV
                     LLVTGTSDAMAPVNRVRDTYHLLSAAGVSARFLELPGPGDHAALLQDAERLHGPLSDFL
                     RRC"
     gene            complement(4296..4931)
                     /gene="udk"
                     /locus_tag="OZ403_RS21620"
                     /old_locus_tag="OZ403_21620"
     CDS             complement(4296..4931)
                     /EC_number="2.7.1.48"
                     /GO_function="GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="udk"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554162.1"
                     /locus_tag="OZ403_RS21620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21620"
                     /product="uridine kinase"
                     /protein_id="WP_140792544.1"
                     /transl_table=11
                     /translation="MASPLVVGIAGGTASGKTTVARKVREALADCRVAFIDQDSYYRDL
                     KDLPLADRREVNFDHPDAFDRELLVQHLQALKSGQPIQKPVYDFVTSSRTPQTKNVDPG
                     DIILIEGILVLHMKEVRDVMDVKIYVDADDDLRILRRLTRDIKDRGRDFDHVVSQYLRH
                     VRPMHMGFVEPSKHFADIIIPHGGNNEIAIGMLVGALRGKLSGAVKRD"
     misc_feature    complement(4984..4998)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.52"
                     /tool="antismash"
     gene            complement(5001..6653)
                     /locus_tag="OZ403_RS21625"
                     /old_locus_tag="OZ403_21625"
     CDS             complement(5001..6653)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638453.1"
                     /locus_tag="OZ403_RS21625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21625"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_268781615.1"
                     /sec_met_domain="DUF692 (E-value: 1.9e-23, bitscore: 81.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHAVSETWTLPWRGLGLSSNLSVADVPQPYRLLDESPGLFDYVE
                     YSAPLSLEESKAHASLFPEMWRRRQDVPVLFHPVHLNLWGPALEPGQALRDLDAHARAV
                     ESPWVGNDVGWWHVDGQPFPGYLYFTPPFNEAGLEACVSHALHVQRHLSVPLVLENPAV
                     LARRGQWHVLDFMAKLHARTGAPLLLDLGHLLSHQLSAGLPLESGLDGFPLDQVIELHI
                     AGGVVTHRGPRSFYVDDHTQPVREELFDLLASLIPRCPSLRAVTFEGDGHPPEVARMSL
                     RRLRQLVPSGARPPLTWCVPAAVAAVPPLTEDSQPWALFDAGYAAAPAASVEDEAGTLA
                     DQDFRLAVVAEVLDRDWPLTRLLLAGTREGLAAFTGSKEYRKLFDSVTRTPGQSFASWA
                     MRRLRESPDEGASAALTLEMLLPSLFTRRVPAPAPGQVGLMEDVRVGSLPVDLSELVHS
                     ARAVRRHLTARAWACGTLELSGLDSLNQVARRPGPGPWSFVARRKAGGPEVMSVPPALG
                     EFLGMLAVRPMAEAEVSPGLLEAARGRGLIRRG"
     gene            6770..7585
                     /locus_tag="OZ403_RS21630"
                     /old_locus_tag="OZ403_21630"
     CDS             6770..7585
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940771.1"
                     /locus_tag="OZ403_RS21630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21630"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_140798707.1"
                     /transl_table=11
                     /translation="MPAPSRVEIDESNAQYRGAWRLFALGSKSGEVVERPEVYITACHV
                     AWSMMNSAFLRAPVETEQALAAAAASAARYFSAGKHGWSFVICDDWLAPSVRDNAPSIL
                     AWYGLKSEMGVTGMVADRLAPAIHPQPPFAVRPVTDTWGRQAVADINANAYDVPRHFGR
                     EAFDEDTLYTPDCQGFVACRDEEAAASTVVLRVDAAAYVALVATQPQHRRQGAAEAVMR
                     HALAKAHQDWGTERTVLHATSAGEPVYTRMGYRPVTRFRMYMAPPPGQG"
     misc_feature    complement(7647..7665)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.38"
                     /tool="antismash"
     gene            7816..8649
                     /locus_tag="OZ403_RS21635"
                     /old_locus_tag="OZ403_21635"
     CDS             7816..8649
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940772.1"
                     /locus_tag="OZ403_RS21635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21635"
                     /product="hypothetical protein"
                     /protein_id="WP_237077290.1"
                     /transl_table=11
                     /translation="MAKWDDLVLAIPPHAGGRYQAPAAVTLLETLLGLHEAQLTLLRGL
                     HRDVRPLREGPFHAGRLHLEAVRAPQATRDEQLERLKSARSCFTEAMGTERDKARLSFI
                     ALHLGLCWLLLGAERDAREWTQLAHRTAVEAMKDILVEASKHQGLTQNRYLDPALTFFM
                     FFTSTATLGAGYLFWDRVLSKRTGTVMRRALGQMQALADYVDMLGEVRLRLGELPHDVA
                     RYELTHGVDEQQSVARITIRKLISDREAIYFNGRETRKVTWNDQERSEVITRELI"
     gene            8947..9639
                     /locus_tag="OZ403_RS21640"
                     /old_locus_tag="OZ403_21640"
     CDS             8947..9639
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554167.1"
                     /locus_tag="OZ403_RS21640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21640"
                     /product="response regulator transcription factor"
                     /protein_id="WP_140792552.1"
                     /transl_table=11
                     /translation="MAERPTVLVVDDDPHLREIVRFALEQGGFRVDEAADGRAALTQVD
                     RALPALIVLDIMMPELDGLAVCREVRRKHELPIVFLSSRDDEVDRILGLELGGDDYLTK
                     PFSPRELVARVKAVLRRARPAPEVEVPAARMQSRGPLRMDAERWRAWWQDTEVVLTVTE
                     FQLLATLLRVPGKVFTRDELMNRVYDDVVVSDRTIDSHVRRVRQKFASAGGDVIETVHG
                     LGYRLALP"
     gene            9608..11248
                     /locus_tag="OZ403_RS21645"
                     /old_locus_tag="OZ403_21645"
     CDS             9608..11248
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940774.1"
                     /locus_tag="OZ403_RS21645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_21645"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_268781616.1"
                     /transl_table=11
                     /translation="MALAIGSPSPERRARPRLWLVFAAVGVAGFALTLAGLAFVRVHDN
                     QLIRQTESELIAQGAVVAEVFRERLRATVPEGVYGRERTAPWPFPIPDDTRLRPILPSL
                     RASDASLPPGDTPRPSLVPAEPLSQAAGLQLTPLLEQVRAATLAGIRVVDTEGVVVASS
                     SPALLWTTLADRTEVQRALRGEPVSVMRRRVADPEDTPLASLSRDTGIRVTVALPVLEG
                     DRVWGAVVLTRTPMTFAKATYADRWNLTATGFVLLGAVALMSLAAAALVGRPVRALVKQ
                     TRAIAMSDPAGFEPMARPVVAELAELSESLAGMATALRDRNQYIRSFAANVSHEFKTPL
                     ASIQGAVELLRDSADAMSPEQHARFLANVDADARRLTRLVQRLLELARADSMTATLAQV
                     ELGPLLEGLALRARAEGATDIHVTAPPTGLKVSLPQEVLDDVLWQLVTNARQHGGDTIR
                     VDLSVEQAPEADMVRVVVKDNGKGISEANRARIFDAFFTTARERGGTGLGLTIAQSMLR
                     AFHARLELLPSDTGTTFAVVAPVPRLQRK"
ORIGIN
        1 ggaattccga acggttacaa aaatgtaggt ccgtataggc ggcggatgct agccatcgct
       61 aaactggctt gtcaacgaac gcaagatgac ccgcatacgc atcggacagc gctggaaacg
      121 cgaacccgcg gcttccccgc tcgattccat cgcactggaa ttggacgggg tggaccttct
      181 gtccggggcc gtggaggagt ctctagcaga agtcgtcccc tccctggtgc gctcagttgc
      241 ggcgctagcg ggtgggcgtc agaaactggc ccaggtctcc ctgcccgagg cccacctgga
      301 gctggtgctc cgccgcatgg ggccggaggt ggagctgctg gtggccaacc tctcccgccc
      361 tgcccggctg atgcgcccgc ccgtccgggt ggagctggag gaactggtga agacggtccg
      421 tgaggccggc gagcgcttcc tcgccgacgt cacccgggcc gggcccaagt cgctggccac
      481 caccgtgggg caagccctca aggagccgct gaaaggcctg aaccggcccg cccgtcctcc
      541 agacgaggcg ccggcccggc ccgccacccg gcgcgtggag cccacggaag tcccgggctt
      601 cggcttcgag ctccgggacg ccggcgtccc catgggccgt ggcgccgcgc gggggacggc
      661 cggactgctg gcgcccctgc tggcatcggg cgaggtctgg ctgtcgctgc ccggaaaggc
      721 cgaggcctgg cgcgtccccg gcccgccgtt cctgacggcg ctggagctgt cgcgacaggc
      781 ggtggagctg gcgcgcgcgg tggagctggg cgaaagccgc ttcgagctgg cccccgcggg
      841 ggcgaagcct tcgctcctgg tcgacctgaa gtcgggccag gcgaaggcgg gacgcaccgg
      901 gacgcccttc ccgctcgcgg gcaacgtgct cgccgccgcc ctcttccacc tgggcgagtc
      961 cctggccgcg gccttcgcgg aggcggaccg cgcgctggtg gccaacccgt accggatgga
     1021 gctggcggag cgctgccgcg agggcctttc gcacctgcgt ggccccgtgc agccccccga
     1081 ggccaggggc gcggcccgag agaagaaggc ccgggccacg ggccagggca cctcacgtcc
     1141 gttgaaggtc cccggccggt tgcgccggct gcgcttcgcg aagctgtggg agaagcgcgg
     1201 cctgccggac gcggaggaat cgaggctgct gctcggccgg cacgggccgg tgtactgcgc
     1261 gccgcagctc gcctccgcgt tctcccgaaa ggacggagcc ctactgtgga agcgcgccgc
     1321 ggcgctgggt gtggccgcgt cggcggatgg ccacgcggtg gcggcggaca tcgcgcgtgt
     1381 ctacggtttc acgggccggg gcggcggtgc gcgctggctg catgaccacg acggaatccc
     1441 cctgggtccg ctgctgctgc gcaaggacgg cctgctgctc accttgtccg aggaccgcac
     1501 cgtcgtggcc ttcgcggagg ccacgggccg cgaggtctgg cgcctggcgc cgccgcggac
     1561 ccagcgcagc tggctggcca cccagggaca ccgcgcgctg gtgggcacgg actcgggcta
     1621 cctctatggg ctcgacctgg aggacggcca ggtccgctac cgcatgcgcg cgcccatgcc
     1681 gttccacggg ccacccgtgg cctggggccg gcgcttcctg gccatgctgg gccgcggctc
     1741 gcactgggcc gtgttgctcg cggatgcgca caccggcgaa tccgtgtgga cgcatgagcc
     1801 ggacctgtcc cacctgtccg cgccgttgcc cgtgggctcg cgggtgttca tcgccggaga
     1861 gcgcgagcgc gaaggcatgc tgctctgcct ggacgcgaag ggccggcgcc tctgggagcg
     1921 cccgctcaac ctgggccctg gcccctatgc gttggcgccc ctcccccgcg cggtggtggt
     1981 gacgagcgcc tcgggcgccg ccacacgggt ggccgcatcc ggcacggtgg actggcgcgt
     2041 gggcgccgcg ggcgagccgc tcatcagcgc gctccccgcc cataccgcgc gcgacgtcac
     2101 gctggtgcca ggggagcgcg tccgcgcggt ggacccccgc ggcgggcagg tgctcgcgga
     2161 ggtccaggcc ggcgtggggc tcgtcgcgct ccaggccgac gtgcgcctca acctctactt
     2221 cctggacgat gccggcacgc tgtccgcgta ccggctcggc tctcacttca cggtggtgga
     2281 gtaacccccg cgaaggccta gttcgccgcg gccttcgacg ggtcgatctc ctcctcgggg
     2341 cgctcttcct gggtggcgcc ctcccaggac tcgccggcgc cgagcttcca ttccgacggc
     2401 acgtccagct tccacacgta ggtggacgcc gcggtgccgc ccgacgccgc gctggaggag
     2461 aggttgatgg tcatctccac cttcgccacc tcgttcggga gcaggtccag gtcgtagtgg
     2521 cccagcacca tctgcggggg gaacgcccgg acgaagcgct ccgggtggtc aatcttcatg
     2581 gacgggtcgg cgccgcgcat ctcgcccagc agcttcccct tcgcgtcggt gagcttccac
     2641 accgtgtcga acgtggcgct ggtcatcatc ttcagcacct tgccgtcgtc cttgagcaca
     2701 cgctgcgcgc cccacaccgc cagttgcagg cggatctgcg gcttgcccag caccatcacc
     2761 acgtcggagg agatgatgtc caggcgcatg cccttgtccg tggccgtcca caccgggcgg
     2821 taacggccct cgcgcaggct gtcgaacgtc ttgcgcgtgt actcgtagaa ggccttgcgg
     2881 tcgttggcac ggtcatcctt cgcgccactc tcctcgcgct tctccagctc caggagatag
     2941 tcgtcgaact tcgtgttccc cttgaaacgc gtgaggtgcg cgtccacctg ctcgaagtag
     3001 tgcttgaaga agggcttcaa ctcatcgcgg tagctggcct cgtccgggtt ggcgcgcatc
     3061 cagcccaccc gttcgaggta ctccgcgtga atccgttgct gatccgcatc ccgctgggct
     3121 tccagcgcgc gaccgctcgc gctgcgatag ctcatgacgg cggccagcag cacacccgcg
     3181 acgatgacga tgactccgaa gtagcgcttc acgcggcagt ctcctgtgcc tttgtcctgg
     3241 cagtgatatg aaaaatcccc aacgtgatcc aggccaccgg gacccacctg ttcaccacgc
     3301 cccttccctt ggaggaagga gagctgctgg gcaatcccca ggtggcctgg gcggcctacg
     3361 gagaaccatc agacggcaaa gcggtggtgt tgctgcacga cctcgcgcac tcgcacctcg
     3421 cgctgggccc gccggaggac tcggcgtacc agccctccgg ctggggacgc gccctggtgg
     3481 ggacgaacct cgcgttggac cccgccatca cccccgtctt ctcccccggg ttgctcggca
     3541 gccctttcgg tagcacgtcg cccgcaacgg tgaatcccgc gtcgggtgaa cgctgggggc
     3601 tcgagctgcc gccgctcacg gtgcaggaca tggcgcgcgc cgtgtcggcc acgctgcgcg
     3661 cgcggggact gacgcaggtg cgcgcgctgg tgggcgtggg actgggcgcc catgtggcgc
     3721 tgcgactggc gtcgctgttc cccgacctgg ccgagggcgt ggtggccatc ggcgcggcgc
     3781 gcgcgctgcc cgaaggcgtg cgcgagaagc tgggcctgtc ctggcaggtg ctgcgcgcgg
     3841 atccggacta ccgggacggc ctctacgcgc tggacgcgcc gccgcgaaag acgatgcgca
     3901 agctgcgtct ggacttcctg aagctgctct gcggacgtga gttcctggcc tccgcgtacc
     3961 cggcccccga cgcggcccgc gccgcgctgg agactgaagc ggacgccttc gcggacgcct
     4021 tcgacgcggt gtcctgggcg tcgctgtgct cggcgtacgc gggcagtgac gtgtcggacg
     4081 gcttctcgcg aatccgtgcg cgggtgctcc tggtgacggg gacgtcggat gccatggccc
     4141 cggtgaaccg ggtgcgtgac acctaccacc tgctgagcgc cgcgggcgtg agcgcccgct
     4201 tcctcgagct gccggggccc ggggaccacg cggcactgct ccaggacgcg gagcggctcc
     4261 atggcccgct gagcgacttc ctgcggcggt gctgactagt cccgcttcac ggcgccggag
     4321 agcttgccgc gaagcgcgcc caccagcatg ccaatggcaa tctcgttgtt gccgccgtgc
     4381 gggatgatga tgtccgcgaa gtgtttggac ggctccacga agcccatgtg catgggccgc
     4441 acgtggcgca ggtactggct caccacgtgg tcgaagtcgc ggccgcggtc cttgatgtcg
     4501 cgggtgaggc gccggaggat gcgaaggtcg tcgtccgcgt cgacgtagat cttcacgtcc
     4561 atcacatccc ggacctcctt catgtggagc accaggatgc cctcgatgag gatgatgtcg
     4621 ccggggtcca cgttcttcgt ctgcggcgtg cgcgacgaag tgacgaagtc gtagacgggc
     4681 ttctggatgg gctgcccgga cttcagcgct tgcaggtgct gaaccagcag ctccctgtcg
     4741 aaggcatccg ggtggtcgaa gttcacctca cgcctgtccg ccagcggaag gtccttcaag
     4801 tcccggtagt atgaatcctg atcgatgaag gccacgcggc aatcggccaa tgcctcgcgt
     4861 accttgcggg caacggtggt cttgccggac gcggtgccgc ccgcgattcc gacgacgagg
     4921 ggggacgcca tgtgggcgcg acgtatctta ccaggacgac cagcgcaagg gttcggtcca
     4981 atcctgaaca attcttcccg ctatccccgg cggatcagcc cacgccccct cgccgcctcc
     5041 agcaacccag gggacacctc cgcctcggcc atgggccgca cagcgagcat tcccaggaat
     5101 tcacccaagg ctgggggcac cgacatcacc tccggtcctc ccgccttgcg ccgagcgacg
     5161 aaggaccagg gccccgggcc cggccggcgc gccacctggt tcagcgaatc caggccggac
     5221 agctccaacg tgccacacgc ccacgcacgg gccgtgaggt gccggcgtac ggctcgggcg
     5281 gaatggacca gctcggacag gtccacggga agcgacccga cccgcacgtc ctccatcaac
     5341 cccacctgcc ccggcgcggg cgcgggcacc cgccgcgtga acagcgacgg cagcagcatc
     5401 tccagcgtca acgccgccga cgccccctca tccggtgatt cgcgcagccg gcgcatggcc
     5461 caggacgcga aggactgccc gggcgtccgc gtcacggagt cgaagagctt ccggtactcc
     5521 ttcgagccgg tgaaggccgc cagcccctcc cgcgtgcccg cgagcaacaa ccgggtgagt
     5581 ggccaatcgc ggtcgaggac ctccgcgacc acggccaggc ggaagtcctg gtccgccagc
     5641 gtgccggctt cgtcctccac ggaggccgcg ggcgcggcgg cgtaacccgc gtcgaacaag
     5701 gcccagggct ggctgtcctc cgtgagtgga ggcacggccg cgacagccgc cggtacgcac
     5761 cacgtcagcg gaggacgcgc tcccgagggc accagctggc gcaggcggcg caaggacatc
     5821 cgggcgacct cgggcgggtg gccatctccc tcgaaggtga cggcacgcag cgacgggcag
     5881 cgcggaatca acgaggccag caggtcgaac agctcctcgc gcaccggctg cgtgtggtca
     5941 tccacgtaga agctgcgcgg cccccggtgc gtcaccacgc ctccggcgat gtgcagctcg
     6001 atgacctggt cgagcggaaa gccgtccagc cccgactcca atggcagtcc cgcggagagc
     6061 tgatggctga gcaggtgccc caggtcgagc agcagcggcg ccccggtgcg cgcgtgcagc
     6121 ttcgccatga agtcgagcac atgccactgc ccgcgccgcg cgagcaccgc ggggttctcc
     6181 agcacgagcg gcacggacag gtgccgctgg acatggagcg cgtgggacac gcacgcctcc
     6241 aggcccgctt cgttgaaggg cggcgtgaag tagaggtagc ccgggaaggg ctgcccgtcc
     6301 acgtgccacc agcccacgtc gttgcccacc cagggactct ccacggcgcg cgcgtgggcg
     6361 tccaaatccc tcaaggcctg gcctggctcc agcgcgggcc cccagaggtt gaggtggacg
     6421 ggatggaaga gcaccgggac gtcctggcga cggcgccaca tctccgggaa caacgaggcg
     6481 tgcgccttcg actcttccaa ggagagcggc gcgctgtact ccacgtagtc gaagaggccc
     6541 ggtgactcgt ccagcaagcg gtagggctgc ggcacgtccg ccacgctcag gttgctgctc
     6601 aaccccaggc cccgccaggg cagtgtccac gtctcggaaa ccgcatgcgt catccgctca
     6661 acgtagacgc gccccgcgtc caccgccacc cgcgactgcc gggcagtggc gcgctgaacg
     6721 ctcccgaggt cgggtatgct cgccgattcc cctcacccca ggagccacga tgcccgcccc
     6781 ctcacgcgtc gagatagatg agtccaacgc gcagtaccgt ggagcctgga ggctgttcgc
     6841 actgggcagc aagtcgggtg aagtggtgga gcgccccgag gtctacatca ccgcctgcca
     6901 cgtagcctgg tcgatgatga actccgcctt cctgcgcgcg cccgtcgaaa cggaacaagc
     6961 gctcgcggcc gcggcggctt cggcggcgcg ctacttctcc gccggcaaac atggctggag
     7021 cttcgtcatc tgcgacgact ggctggcgcc ctccgtgcgc gacaacgcgc cctccatcct
     7081 tgcctggtac gggctcaagt ccgagatggg cgtcaccgga atggtggcgg accggctggc
     7141 gcccgccatt cacccacagc ccccgttcgc cgtccgtccc gtcacggata cctggggccg
     7201 gcaggccgtg gccgacatca acgccaacgc ctacgacgtc ccccgccact tcggccgtga
     7261 agcgttcgac gaggacacgc tctacacccc ggactgccag ggcttcgtgg cgtgccgcga
     7321 cgaagaggcc gcggccagca cggtggtgct gcgcgtggat gccgccgcct acgtcgccct
     7381 cgtcgcgacc cagccgcaac accgccggca gggtgccgcc gaggccgtga tgcgacatgc
     7441 gctggcgaag gcccatcagg actggggcac cgagcgcacc gtcctccacg cgaccagcgc
     7501 gggcgagccc gtctacaccc gcatgggcta ccggccggtg acgcgcttcc gcatgtacat
     7561 ggcgcctcca ccgggccagg gctgagccac gcgcgagggc tcggcacggg ggagtaaaaa
     7621 cagactcacg ccatttcgag agagtgactg gctttttcta ccgagcttga aagccagtcg
     7681 ccccacctca ttgaagaatg ccgctctcgc atgaactgcg gcccacattc ttcgatggcg
     7741 tggaggttat ggcacactga tccgtgaggg ggcttgccgc acgtcttgca tcgcccagcc
     7801 acacggagtc cgtacatggc caagtgggat gatctcgtcc tggcgattcc accgcacgcg
     7861 ggcggcagat atcaggcgcc cgccgcggtc acgctgctgg agacactcct cggccttcac
     7921 gaagcacagc tcaccctcct tcgcggattg catcgcgacg tccggccgct gcgggaaggt
     7981 cccttccatg ccgggcggct ccacctggag gccgtgcgcg cccctcaggc cacccgcgac
     8041 gaacagctgg agcggctgaa gtccgcgcgg agctgcttca cggaagccat gggcaccgag
     8101 cgcgacaagg cgcggctgtc gttcatcgcc ctgcacctgg gcctctgctg gctgctgctg
     8161 ggcgcggagc gggatgcgcg ggagtggacg cagctggccc accgcacggc cgtggaggcc
     8221 atgaaggaca tcctggtgga agcctcgaag caccagggcc tcacccagaa ccggtacctg
     8281 gatccggcgc tcaccttctt catgttcttc accagcacgg ccacgctggg cgcgggctac
     8341 ctcttctggg accgggtgct cagcaagcgc acgggcacgg tgatgcggcg cgcgctgggg
     8401 cagatgcagg cgctggcgga ctacgtggac atgctgggcg aggtccgcct ccgcctgggc
     8461 gagctccccc atgacgtcgc ccggtacgag ctgacccacg gcgtggacga acagcagtcc
     8521 gtcgcccgca tcaccatccg gaagctcatc agcgaccggg aggccatcta cttcaacggc
     8581 cgggagaccc ggaaggtcac ctggaacgac caggagcgct ccgaggtcat cacccgcgag
     8641 ctgatctgac gccgggcgct gtcaccgtcc cgtcaaaacc tgttttcagg attgccgcga
     8701 gcgaaacgtc agaccggccc gccatagtag cggcacttcc tcgatggggg cgaaggtgac
     8761 ggcgcaaacg atgctcgtgt tcattccagt gattctccta ctcgccgcgg cgattgcgat
     8821 tccgcggccc cgccggcgct cacgccggta gtcgtagtca cggccggggt tgcgccccgg
     8881 ccgtctcccc ggcccgggcg ggtgtagagt gcggcagtcg tcgcaatcct cccctcccga
     8941 gcagccgtgg ccgaacgtcc cacagtcctg gtcgtcgacg atgatcctca cctgagggag
     9001 atcgtccgct tcgcactgga gcagggaggc ttccgggtgg atgaggccgc ggacggccgc
     9061 gccgccctga cgcaggtcga ccgggccctc ccagcgctca tcgtcctgga catcatgatg
     9121 ccggagctgg acgggctcgc cgtgtgccga gaggtccggc gcaagcacga gctgcccatc
     9181 gtcttcctct cctcacggga tgacgaggtc gaccgcatcc ttggcctgga gctgggtggc
     9241 gacgactacc tcaccaagcc cttcagtccg cgcgagctcg tggcccgcgt gaaggcggtg
     9301 ctccggcgcg cgcggcccgc cccggaggtc gaagtgcccg cggcgcggat gcagtctcga
     9361 gggccgctgc gcatggacgc ggagcgctgg cgcgcgtggt ggcaggacac ggaggtggtg
     9421 ctcacggtga cggagttcca gctcctggcc acgctgctcc gagtccccgg caaggtcttc
     9481 acccgggatg agctgatgaa ccgcgtctac gacgacgtgg tggtcagcga ccggaccatc
     9541 gacagtcacg tgcgccgggt ccggcagaag ttcgccagcg cgggcggaga cgtcatcgag
     9601 acggtgcatg gccttggcta tcggctcgcc ctcccctgag cgccgcgcgc gcccgcgctt
     9661 gtggctggtg ttcgccgccg tgggcgtcgc ggggttcgcc ctcacgcttg cggggctcgc
     9721 cttcgtgcgc gtccatgaca accagctcat ccgccagacg gagtcggagc tgattgccca
     9781 gggcgcggtg gtcgcggagg tcttccgcga gcggctgcgc gccaccgtgc cggagggcgt
     9841 atacggccgc gagcgcaccg ctccctggcc cttccccatt cccgacgaca cgcgcctgcg
     9901 ccccatcctc ccgtcgctca gggcctcgga tgcgtccctt ccgccgggcg acacgcctcg
     9961 gccttcgctc gttcccgcgg agccgctgtc acaggcggcc ggactgcagc tgacgccgct
    10021 gctcgaacag gtgcgcgcgg cgaccctggc cggcatccgc gtggtggaca ccgagggcgt
    10081 ggtggtggcc agcagcagcc ccgcgctgct gtggacgacg ctggcggacc gcaccgaggt
    10141 ccagcgggcc ctgcgcggcg agcccgtcag cgtgatgcgc cgccgcgtcg ccgatcccga
    10201 ggacacccca ctggcgtcac tcagccgcga caccggcatc cgcgtcacgg tggcgctgcc
    10261 cgtactggaa ggcgatcgcg tctggggcgc ggtggtgctg acgcgcacgc ccatgacgtt
    10321 cgccaaggcg acgtacgcgg accggtggaa cctcaccgcc accggcttcg tgttgctcgg
    10381 cgcggtggcg ctgatgtcgc tggccgccgc cgcgctggtg ggccggccgg tgcgcgcgct
    10441 ggtgaagcag acgcgcgcca tcgccatgag cgaccccgcg ggcttcgaac ccatggcgcg
    10501 ccccgtggtg gcggagctgg ccgagctctc cgagtccctg gccggcatgg ccaccgcgtt
    10561 gcgagacagg aaccagtaca tccgttcctt cgcggccaac gtgtcgcacg agttcaagac
    10621 gccgctggcc tccatccaag gcgcggtgga gctgcttcga gacagcgccg acgcgatgtc
    10681 tcccgagcaa cacgcgcggt tcctcgccaa cgtggacgcg gatgcccgca ggctcacccg
    10741 cctggtgcag cgcctgctgg agctggcccg tgcggactcc atgacggcca cgctcgcgca
    10801 ggtggagttg ggcccgctgt tggaggggct cgcccttcgc gcccgcgcgg aaggcgccac
    10861 ggacatccat gtgacggcac cgcccacggg actgaaggtg agcctcccgc aggaggtgct
    10921 cgacgacgtg ctgtggcagc tcgtcaccaa tgcccgccag cacggtggcg acaccatccg
    10981 cgtggacctg tcggtggagc aggccccgga ggcggacatg gtgcgcgtgg tggtgaagga
    11041 caacggcaaa ggcatctccg aggccaaccg tgcgcgcatc ttcgacgcct tcttcaccac
    11101 cgcccgcgag cgaggcggca cgggcctggg cctgacgatt gcccagtcca tgctgcgcgc
    11161 cttccacgcg aggttggaac tgcttccttc cgacacgggc accaccttcg cggtggtggc
    11221 gcccgtgccc cggcttcaac ggaagtagct gtcggagatg tccacggagt agcccagctc
    11281 cagcgtgggc gaggacgcgg ccaccaggaa ctggcggaaa tgcagcgcct ttccgggcag
    11341 gcctgacacc tccgcggcca cggctcccgt cgactcgtac tgctccaggc tgccgccctc
    11401 gcgccggtag ttgaacaccg ctgggaagtg cgcgcgtgca cggagctccg ccaccggcca
    11461 ggagactcgc ggcaacgcca gcttcaaccg gccggagagg tggaagacgc ccagctctcg
    11521 cgacagcacc acctccagcg tcccgcccgt ctctcccagc cgcgccggag tggccttcaa
    11581 cgccagggtg ccgttctccg actccacggg gacgggaatg gcgttcaggg tgacgcgctc
    11641 gagccggtgt cct
//
