LOCUS       NZ_CP113950            10864 bp    DNA     circular CON 28-JAN-2024
DEFINITION  Myxococcus sp. NMCA1 chromosome, complete genome.
ACCESSION   NZ_CP113950
VERSION     NZ_CP113950.1
KEYWORDS    RefSeq.
SOURCE      Myxococcus sp. NMCA1
  ORGANISM  Myxococcus sp. NMCA1
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1  (bases 1 to 9502182)
  AUTHORS   Ahearne,A., Stevens,C. and Phillips,K.
  TITLE     Minimal conservation of predation-associated metabolite biosynthetic
            gene clusters underscores biosynthetic potential of Myxococcota
            including descriptions for ten novel species: Archangium lansinium
            sp. nov., Myxococcus landrumus sp. nov., Nannocystis bai
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9502182)
  AUTHORS   Ahearne,A., Stevens,C. and Phillips,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2022) Biomolecular sciences, University of
            Mississippi, Faser Hall 407, Oxford, MS 38655, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 10.1.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: PacBio Sequel II
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 03:49:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,878
            CDSs (total)                      :: 7,793
            Genes (coding)                    :: 7,662
            CDSs (with protein)               :: 7,662
            Genes (RNA)                       :: 85
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 56 of 131
            Pseudo Genes (incomplete)         :: 88 of 131
            Pseudo Genes (internal stop)      :: 13 of 131
            Pseudo Genes (multiple problems)  :: 24 of 131
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:28:59
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4275544
            Orig. end                         :: 4286408
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP113950.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10864
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10864
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10864
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5864](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5864)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    18..27
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     gene            166..996
                     /gene="rsmI"
                     /locus_tag="OZ403_RS18410"
                     /old_locus_tag="OZ403_18410"
     CDS             166..996
                     /EC_number="2.1.1.198"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rsmI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553581.1"
                     /locus_tag="OZ403_RS18410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_18410"
                     /product="16S rRNA (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="WP_268784715.1"
                     /sec_met_domain="TP_methylase (E-value: 2.2e-29, bitscore:
                     101.8, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGTLYLVATPIGNLGDVTSRALETLRAVRFIACEDTRHSRVLLD
                     HFGIGGKDLVSLPAFAEGQRAGRILDRIGEGEDCALVTDAGSPAISDPGEKLVSEALER
                     GLMVVPVPGPTALVAALSASGLPTGRFHFLGFLPRKGAERRAMLEEVAQLSATLVLYES
                     PRRLSETLPDLLDAWGDRRACVARELTKLHEEFARGTLSELAARYAAEEPRGEVVVLVE
                     GRTGETRWSEEELRRALEEGLSRGEKLKALSTELARRAGWAGQDVYRLGLSLKR"
     gene            complement(1040..2335)
                     /locus_tag="OZ403_RS18415"
                     /old_locus_tag="OZ403_18415"
     CDS             complement(1040..2335)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553582.1"
                     /locus_tag="OZ403_RS18415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_18415"
                     /product="hypothetical protein"
                     /protein_id="WP_140791572.1"
                     /transl_table=11
                     /translation="MHRLRVVLAALTLGAPFAATAADITRIASSLEEDDPFDLFIDVGF
                     ERTQNRAKIIREQLAPAGQPGGRVDASELWYKGVDARLNLELSVGLYRDLEFSFALPVV
                     FSQNERWDFTSNSSPETSTITNNCLNANGSAIPGCLEGSAPGNALFDVPQESFRGGLGN
                     MRFGLAYAFFNQEKDETKPTWIVGLEYEAPTAKLRDAAADTTDSDNRGNIGDRVHKYQL
                     YTAFSRRIGAAEPYFKAHYTIPVRGPGAYSNCAQAESNPDNLGAPGNCFTGPWNRKETG
                     INAPHTAGMLFGVELVPFNRPAKSQRFALDVRGIGNYVGSGRYYNELSAALRKLLTSED
                     YFQVGGMLGVTANASKAFQIRASGMFLYNTNHAITAEEPGRDVNNDGAVDLALNSPELN
                     PNFDWRYDLVSRRFRAVESTTFRFDLSATFSF"
     gene            complement(2432..3469)
                     /locus_tag="OZ403_RS18420"
                     /old_locus_tag="OZ403_18420"
     CDS             complement(2432..3469)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349973.1"
                     /locus_tag="OZ403_RS18420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_18420"
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="WP_174258811.1"
                     /transl_table=11
                     /translation="MRPNLPTLGAPPKRSPFGTVVAVSLILGGAAGGVWWWKQRMVQAT
                     EEAAAQMPNAVPDAGAVAAAPTPAPVATDPVKAAGLERVSVSINGPLETALVNAAGSDV
                     GPALAQVVTRTLVWWVSVPGEILRGDTLEVLYQRRTNEEPLVHAVRFTSAKLGKTMSAY
                     RYQGSGEPNARYYLSGGDELELRLERSPIDDYEQVTSLLRDGRKHKGVDFRTPVGTPIK
                     APFNGVVKRKNWNFSSNGNCIELVESGGKGRRALFLHLDEVDKSVKPGTRFSVGQVIAK
                     SGNTGRSFAPHLHYQLMTQDDRVLDPYDQHKTYRRSLAAQHRSGFEAEMRRMDGLLSAS
                     VVAGK"
     gene            complement(3546..4970)
                     /locus_tag="OZ403_RS18425"
                     /old_locus_tag="OZ403_18425"
     CDS             complement(3546..4970)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940152.1"
                     /locus_tag="OZ403_RS18425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_18425"
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="WP_268784716.1"
                     /transl_table=11
                     /translation="MAKVLVIDDEANLRKVLATMLRRDGFDVTVAENGEQGLAEFHKNG
                     ADIVVTDLVMPKVGGMEVLGTIRAANPDVPVIIITAHGTVDSAVDAIKAGAFDYITKPF
                     DQAELSSVVAKAAKTNESARRSVRPDVKARAAIIGDSSTIQEVYKIIDKVADTPSTVLI
                     TGESGTGKELIATALHGASSRRDKPFIKINCAAIPHTLLESELFGYERGAFTGAVTSKP
                     GRFELADEGTLFLDEIGEIPVEMQVKLLRALQEGEFERVGGIKTTRVDVRLVAATNRDL
                     QAEIEAGRFRKDLYYRLAVVPISLPALRERRSDIPMLARHFVEKYNRRLNKKIEGIADD
                     AMALLQGYGWPGNIRELENLIERVLLFADGPLITARDLPEPVRQGTSVQAGANLASAVA
                     SIDVPTGEVGLKDIVRMKAAELERDLIVKKLEETGGNVTRAARLLQISRKSLQTKMKEF
                     GLRDTTPDGQEDGPDE"
     gene            complement(5001..5864)
                     /gene="encA"
                     /locus_tag="OZ403_RS18430"
                     /old_locus_tag="OZ403_18430"
     CDS             complement(5001..5864)
                     /codon_start=1
                     /gene="encA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114001.1"
                     /locus_tag="OZ403_RS18430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_18430"
                     /product="encapsulin nanocompartment shell protein EncA"
                     /protein_id="WP_141254361.1"
                     /sec_met_domain="Linocin_M18 (E-value: 3.8e-83, bitscore:
                     277.3, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDFLGHAENPLREEEWARLNETVIQVARRSLVGRRILDIYGPLG
                     AGVQTVPYDEFQGVSPGAVDIVGEQETAMVFTDARKFKTIPIIYKDFLLHWRDIEAART
                     HNMPLDVSAAAGAAALCAQQEDELIFYGDARLGYEGLMTANGRLTVPLGDWTSPGGGFQ
                     AIVEASRKLNEQGHFGPYAVVLSPRLYSQLHRIYEKTGVLEIETIRQLASDGVYQSNRL
                     RGDSGVVVSTGRENMDLAVSMDMVAAYLGASRMNHPFRVLEALLLRIKHPDAICTLEGA
                     GATERR"
     gene            complement(5911..6390)
                     /gene="encB"
                     /locus_tag="OZ403_RS18435"
                     /old_locus_tag="OZ403_18435"
     CDS             complement(5911..6390)
                     /codon_start=1
                     /gene="encB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114000.1"
                     /locus_tag="OZ403_RS18435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_18435"
                     /product="encapsulin nanocompartment cargo protein EncB"
                     /protein_id="WP_174258812.1"
                     /transl_table=11
                     /translation="MAGPPDSDLDDVARIRLVLARELETINEYEAYARASSHPEVRAFF
                     QHLAAEEKEHVSEAVHMLRMLDSGQNDHFAKPFVPGHFQAAEPPAPTPASVHVPAPDGP
                     AFSVNGRNGRLPSEPPTSLPPQRLLYGLPAPPPAVESHPLTVGSLRRGGGSGSGR"
     gene            6496..8193
                     /gene="murJ"
                     /locus_tag="OZ403_RS18440"
                     /old_locus_tag="OZ403_18440"
     CDS             6496..8193
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="murJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940155.1"
                     /locus_tag="OZ403_RS18440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_18440"
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="WP_140875145.1"
                     /transl_table=11
                     /translation="MTTSSPESPPPPIAPVAPPRPERSGGGAALVAAGILASRLMGLVR
                     ERVFAHYLGNAEAAAVFKAALRIPNFLQNLFGEGVLSGSFIPVYAQLLGRKDTETADRV
                     AGAVFGILSLVTAVVVALGMVFTPLLVDAIAPGFRGQERELAVHLVRILFPGTGMLVLS
                     AWCLGILNSHRRFLLSYLAPVVWNLVIIAALVAAGGRYEEEALVSVLAYAVVLGSFLQF
                     AVQVPSVLKLLGRFRPTLSLAAEPVRQVLKNFGPVVLGRGVVQFSAWVDTAFASLISNR
                     ALSSLLYAQTIYLIPVSLFGMAVSAAELPEMSRATGEGADVAARLRQRIDGGARRICFW
                     VVPSAAAFLFLGDMVAAALLQTGRFDAADSRYLWYLLMGAALGLVASTVGRLYASAFYA
                     LKDPKTPLRFAVVRVLVGTLGAWVLGLRLPGWLGVPQHLGAVGLTLASGLVAWLEAGLL
                     RRKLVKQLGPVGVPSGLLPRLWGAAVVAGLVAVGIKVGLTSLLGPMPGVTAEWGGGFLM
                     PPRLHPVLGFAAVALPFGAVYFGMSAALGIPEAGTVLRKVGRKLRLVR"
     gene            8466..10406
                     /locus_tag="OZ403_RS18445"
                     /old_locus_tag="OZ403_18445"
     CDS             8466..10406
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940156.1"
                     /locus_tag="OZ403_RS18445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="OZ403_18445"
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="WP_268784717.1"
                     /transl_table=11
                     /translation="MGPKKPKATFGDVMLGIPSGGARSEGGRGEQRGGGRGGERGGSGR
                     GEREAQPRPAGGAPRDERGPRGGGERRGGGDRRPSGPMVVVKRASGAIETRGPVGDAPA
                     EATATAEETTAAAESSAATPAPTPRPVTPTPAPTSALYEEVPEAQSFAEMFEAQAKEGG
                     TPSRKGLRVGEKVRGTIFQLGADTAFVSVEGAAKSEAMIELRELKDDEGILRFGVGDTI
                     DAHVVEVGAKGILLSRALAKGSANLALLAEARASGMPVEGLVLSVNKGGVEVAIGDIRA
                     FCPISQLDLRYVEKPDQFIGEKLQFRVSEVRERNVVLSRRALLEDEQRQLAAETRKNLA
                     QGKIVKGKVTGVRDFGVFVDLGGVEGMIPVSELSYTRVGHPSDVVKVGDDVEVEILRME
                     AGQPNSPDKSKQKERITLSMRSRQEDPFQKALSEIKEGDRVQGKVVRLQQFGAFVELRP
                     GVDGLVHISALSDRRIAHPRDVVKEGESIWVSVEKIDTQEKRIGLRRISEEEAQRPPEE
                     RSAPAAEAAAPAQPAAPRPKVGQVVVGKVDRIEPYGVFLAFPGGKGLLPASETGTERGT
                     DLRKHYSLGQEVKVAILDIDASGKIRLSVTAAIRAEERAEVEAWQKTQQPQGAGKKGFG
                     TFADLLSKARK"
     tRNA            10508..10581
                     /anticodon=(pos:4286086..4286088,aa:Met,seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="OZ403_RS18450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="OZ403_18450"
                     /product="tRNA-Met"
     gene            10508..10581
                     /locus_tag="OZ403_RS18450"
                     /old_locus_tag="OZ403_18450"
     tRNA            10649..10722
                     /anticodon=(pos:4286227..4286229,aa:Met,seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="OZ403_RS18455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="OZ403_18455"
                     /product="tRNA-Met"
     gene            10649..10722
                     /locus_tag="OZ403_RS18455"
                     /old_locus_tag="OZ403_18455"
     tRNA            10788..10861
                     /anticodon=(pos:4286366..4286368,aa:Met,seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="OZ403_RS18460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="OZ403_18460"
                     /product="tRNA-Met"
     gene            10788..10861
                     /locus_tag="OZ403_RS18460"
                     /old_locus_tag="OZ403_18460"
ORIGIN
        1 aagcagcgcc gggtggtgaa ggcggcgctt cggtacctgt tcgcgcatga cttgcgtgga
       61 cggatgctcc gtttcgacgt gatttcggtg gtggggcgcg gggagcgcgc caccgtggag
      121 cacatccctg gcgccttcga cgcggggatg tgagaggacg acgccatggc tggaacgctc
      181 tatctggtgg cgacgcccat cgggaacctg ggggacgtca cgtcgcgcgc gttggagacc
      241 ctgcgcgcgg tgcgcttcat cgcgtgtgag gacacgcggc actcgcgggt gctgctcgac
      301 cacttcggca ttggcgggaa ggacctggtg agcctgcctg ccttcgcaga agggcagcgc
      361 gccggacgca tcttggaccg gataggggaa ggggaggact gcgcgctcgt gaccgatgcg
      421 ggcagccccg ccatcagcga cccgggcgag aagctggtat ccgaggcgct ggagcgcgga
      481 ttgatggtgg tgccggtgcc aggccccacg gcactggtcg cggcgctgag cgcctctgga
      541 ttgcccacgg ggcgcttcca cttcctgggc ttcctcccgc gcaagggcgc cgagcgccgg
      601 gcgatgctgg aggaagtggc gcagctgtcc gccaccctgg tgctctatga gtccccacgc
      661 cgcctgtcgg agacgttgcc cgacctgctc gacgcctggg gcgaccggcg cgcgtgcgtg
      721 gcgcgggagc tgaccaagct gcacgaggag ttcgctcggg gcaccttgtc cgagctggcc
      781 gcgcgttacg cggcggaaga gccccgcggc gaggtggtgg tgctggtgga aggccgcact
      841 ggcgagacgc gttggtccga ggaggaactg cgccgggccc tggaggaagg gctgtctcgc
      901 ggcgagaagc tcaaggcgct gagcaccgag ctggcccgcc gcgcgggctg ggccggtcag
      961 gacgtctacc ggctggggct ttcgctgaag cgctgagtcc ctgggtgggc gccgtcatcg
     1021 gcgcccaccg ggccactcat tagaagctga acgtggcgct caggtcgaag cggaacgtgg
     1081 tgctctccac cgcgcggaag cggcgggaca ccaggtcata gcgccagtcg aagttggggt
     1141 tgagctcggg ggagttgagc gcaaggtcca cggcgccgtc gttgttcacg tcacgccccg
     1201 gctcctcggc cgtgatggcg tggttggtgt tgtagaggaa catgccggac gcgcgaatct
     1261 ggaaggcctt cgacgcgttc gcggtgacgc ccagcatgcc acccacctgg aagtagtcct
     1321 cggaggtgag cagcttgcgc agggcggcgc tcagctcgtt gtagtagcgg ccgctgccca
     1381 cgtagttgcc aatgcctcgg acgtccaggg cgaagcgctg cgacttcgct ggccggttga
     1441 agggcaccag ttccacgccg aagagcattc ccgcggtgtg aggggcgttg atgcccgtct
     1501 ccttgcggtt ccacgggccc gtgaagcagt tgcccggggc gcccaggttg tccgggttgg
     1561 actcggcctg ggcgcagttg gagtaggcgc ccggaccgcg cacggggatg gtgtagtgcg
     1621 ccttgaagta cggctctgcc gcaccgatgc ggcgcgagaa ggccgtgtag agctggtact
     1681 tgtggacgcg gtcgccgatg ttgccgcggt tgtccgaatc ggtggtgtcc gcggccgcgt
     1741 cgcgcagctt cgcggtgggg gcctcgtact ccaggccgac aatccaggtg ggcttcgtct
     1801 cgtccttctc ctggttgaag aaggcgtagg ccaggccgaa gcgcatgttg cccaggccgc
     1861 cgcggaagct ctcctgcggt acgtcgaaca gggcgttgcc cggtgccgag ccctccagac
     1921 agcccgggat ggcggagccg ttggcgttca ggcagttgtt ggtgatggtg gacgtctcgg
     1981 gcgacgagtt ggacgtgaag tcccagcgct cgttctgcga gaagacgacg ggcagggcga
     2041 aggagaactc caggtccctg tagaggccga cggacagctc caggttgagc cgggcgtcca
     2101 cgcccttgta ccagagctcc gacgcatcca cgcggccccc cggttgcccg gcgggggcca
     2161 gctgctcgcg gatgatcttc gcgcggttct gcgtgcgctc gaagccgacg tcgatgaaga
     2221 ggtcgaatgg gtcgtcctcc tcaagcgacg acgcaatccg ggtgatgtcc gccgcggtgg
     2281 cggcgaacgg cgcgcccagc gtcagcgcgg caagtacgac gcgaagcctg tgcatccgac
     2341 ctccgacggc aaatctcccc ggccttccgg caagcccggg gggcttccag ctagccgtgc
     2401 cgtcgaagga gtgtcaagaa acgggtggct tttacttgcc ggccaccacg gacgcgctca
     2461 gcaacccgtc catccggcgc atctccgcct cgaaacccga ccggtgctgc gcggccagtg
     2521 agcggcggta ggtcttgtgc tggtcgtagg ggtccagcac ccggtcatcc tgcgtcatga
     2581 gctggtagtg gaggtggggg gcgaagctgc ggcccgtgtt gccgctcttg gcgatgactt
     2641 gccccacgga gaaccgggtg cccggcttga cgctcttgtc cacctcgtcc aggtggagga
     2701 agagcgcgcg gcgacccttg ccgcccgact ccaccagctc gatgcagttg ccgttgctgc
     2761 tgaagttcca gttcttccgc ttcaccacgc cgttgaaggg ggccttgatg ggcgtaccca
     2821 ccggggtgcg gaagtccacg cccttgtgct tgcggccgtc gcggagcagg gacgtcacct
     2881 gctcgtagtc gtcgatgggg gagcgctcca gcctcagctc cagctcgtcg ccgccggaca
     2941 ggtagtaccg ggcattgggc tcgccgctgc cctggtagcg gtacgcgctc atcgtcttgc
     3001 ccagcttggc gctggtgaag cgcaccgcgt gcaccagggg ctcctcattg gtgcggcgct
     3061 ggtagagcac ctccagcgtg tcaccgcgga ggatttcccc ggggacggag acccaccaca
     3121 ccagggtgcg cgtcaccacc tgcgccagcg ccggacccac gtccgagcca gcggcgttga
     3181 cgagcgcggt ctccaggggg ccgttgatgc tcaccgacac gcgctccagg ccggcggcct
     3241 tcaccgggtc cgtggccacg ggcgccggcg tgggcgccgc ggcgacggcg cccgcatcgg
     3301 ggacggcgtt gggcatctgg gccgcggcct cttccgtcgc ctgaaccatc cgctgcttcc
     3361 accaccacac gccgccggcg gcaccgccga ggatgagtga cacggcgacc accgtgccga
     3421 agggactgcg cttcggaggg gcaccgaggg tgggaaggtt cggccgcata caagctccct
     3481 gagttggaat gggtgggcgg gcccggaacc gacgcgcgag aagcggaatt ccgcctcctc
     3541 gcggtctatt cgtccgggcc gtcttcctgc ccgtctggtg tggtgtcgcg caagccgaac
     3601 tccttcatct tcgtctggag tgacttgcgg ctgatttgaa gcaggcgagc ggcccgggtg
     3661 acattgccac ccgtctcctc gagcttcttc acgatgaggt cccgctcgag ctcggcggcc
     3721 ttcatccgga cgatgtcctt caggccgact tcccccgtgg gaacgtcgat ggaggccacc
     3781 gcgctggcca ggttggcgcc cgcctggacg ctcgttccct ggcggaccgg ctccggcagg
     3841 tcgcgggcgg tgatgagggg cccgtctgcg aacaggagca cgcgctcgat gagattctcc
     3901 agctcgcgga tgttgcccgg ccacccgtag ccctggagca gggccatggc gtcgtcggcg
     3961 atgccttcaa tcttcttgtt gaggcgccgg ttgtacttct ccacgaagtg ccgggcgagc
     4021 atggggatgt cgctgcggcg ctcacgcagg gcgggcaggg agatggggac caccgccagg
     4081 cggtagtaga ggtccttgcg gaagcggccg gcttcaatct ccgcctggag gtcgcggttg
     4141 gtggcggcca cgagccgcac gtccacgcgc gtcgtcttga tgccgcccac gcgctcgaac
     4201 tcgccctcct ggagcgcgcg cagcagcttc acctgcatct ccaccggaat ctcgccgatc
     4261 tcgtccagga agagggtgcc ctcgtcggca agctcgaagc ggccgggctt ggacgtgacg
     4321 gcaccggtga aggcccctcg ctcgtagccg aagagttcgc tctccagcag cgtgtgggga
     4381 atggcggcgc agttgatctt gatgaacggc ttgtcccggc ggctggaggc cccgtgcaac
     4441 gcggtggcga tgagctcctt gccggtgccg ctctctccgg tgatgagcac cgtggacggc
     4501 gtgtccgcca ccttgtcgat gatcttgtag acctcttgga tcgtcgacga gtcgccgatg
     4561 atggccgcgc gcgccttcac gtccgggcgc acggagcggc gggcgctctc gttcgtcttc
     4621 gcggccttgg cgacgacgga ggacaactcc gcctgatcga agggcttggt gatgtagtcg
     4681 aacgcgcccg ccttgatggc gtccacggcg gagtccaccg tgccatgcgc ggtgatgatg
     4741 atgacgggga cgtccgggtt ggcggcgcgg atggtgccga gcacctccat gccgcccacc
     4801 ttgggcatca ccaggtccgt caccacgatg tccgcgccgt tcttgtggaa ctcggcgagc
     4861 ccttgctcgc cgttctccgc cacggtgacg tcaaagccgt cccggcgcag cattgtggcg
     4921 agcaccttgc ggaggttcgc ctcgtcgtcg atgaccagga ccttggccat ggtggaagtc
     4981 gtgaccgcgg gcgaccggaa ctagcgacgt tccgtggcgc cggcgccctc cagggtgcag
     5041 atggcgtccg ggtgcttgat gcgtagcagc agcgcctcca gcacgcggaa cgggtggttc
     5101 atccgcgacg cgcccaggta cgccgccacc atgtccatgg acacagcgag gtccatgttc
     5161 tcccggccgg tggacaccac cacgccggag tccccgcgca ggcggttgga ctggtagacg
     5221 ccgtccgacg cgagctggcg gatggtctca atctccagca cgcccgtctt ctcgtagatg
     5281 cggtgcagct gcgagtacag ccgcggcgac aggacgacgg cgtaggggcc gaagtggccc
     5341 tgctcattga gcttgcgcga ggcctcgacg atggcctgga acccaccacc cggcgacgtc
     5401 cagtcaccca gcggcaccgt gagccggcca ttggccgtca tcaggccttc gtaacccagg
     5461 cgcgcgtcac cgtagaagat gagctcgtct tcctgctgcg cgcacagggc ggcggcgccg
     5521 gcggcggccg acacgtccag cggcatgttg tgcgtgcgcg cggcctcgat gtcgcgccag
     5581 tgcagcagga agtccttgta gatgatgggg atggtcttga acttgcgagc atcggtgaag
     5641 accatcgcgg tctcctgctc gccgacgatg tccacggcgc cgggcgacac gccctggaac
     5701 tcgtcgtagg gcaccgtctg caccccggcg cccagcggcc cgtagatgtc caggatgcgc
     5761 ctgcccacca gcgaacgccg ggcgacctgg atgacagtct cgttcagacg cgcccactcc
     5821 tcttcgcgga gcgggttctc ggcatgtcca aggaagtcag gcatgaaatg gggctccaga
     5881 ggcatcgact agagaagggg gaagaaagct tcagcgaccg ctgccggaac caccaccccg
     5941 gcgcagcgaa cccacggtga gcgggtgcga ctccacggcg ggcggcggcg cgggcaggcc
     6001 atacagcagc cgctggggcg gcagggacgt tggcggctcg gaggggagcc ggccgttgcg
     6061 gccgttgacg gagaaagccg ggccgtccgg tgcgggcaca tggacggagg cgggggtggg
     6121 ggcggggggc tcggcggcct ggaaatgacc ggggacgaag ggcttggcga agtggtcgtt
     6181 ctgaccactg tccaacatgc ggagcatgtg aacggcctcg gagacgtgct ccttctcctc
     6241 ggcggccagg tgctggaaga acgcgcggac ctccgggtgg gaggaggccc gagcgtaggc
     6301 ttcgtactcg ttgatcgtct ccagctcacg tgccagcacg aggcggatgc gcgccacgtc
     6361 gtccaagtca ctgtcgggag ggccggccat tggaatggac ccttgcaatc cagggggttc
     6421 gccgtcaagc gaaacagggg gacggggttt cgggcttcat gacaccgaac ggcccgcgta
     6481 gagtccgccg ctctcgtgac cacgtcctcg cccgagtctc cgcccccgcc gattgcgccg
     6541 gtcgcgcctc ctcgtcccga gcgttcaggg ggcggcgccg cgctggtagc ggctggcatc
     6601 cttgcttccc ggctgatggg cctggtgcgc gagcgcgtct tcgcccacta cctgggcaac
     6661 gcggaggccg cggccgtctt caaggcggcg ctgcgcattc ccaacttcct gcagaacctg
     6721 ttcggcgagg gcgtcctctc cggctcgttc atccccgtct acgcgcagct cttgggccgc
     6781 aaggacacgg agacggcgga ccgggtcgcg ggcgccgtct tcggcatcct gtccctggtc
     6841 acggcggtgg tggtggcgct gggcatggtg ttcaccccgc tgctggtgga tgccatcgcg
     6901 cccggcttcc ggggacagga gcgggagctg gccgtccacc tggtgcgcat cctcttcccc
     6961 ggcacaggca tgctggtgct gagcgcctgg tgcctgggca tcctcaacag ccaccggcgc
     7021 ttcctccttt cgtacctggc gcccgtcgtc tggaacctgg tcatcatcgc cgcgttggtg
     7081 gccgcgggag gccgctacga ggaagaggcg ttggtctcgg tgctggccta cgccgtggtg
     7141 ctgggcagct tcctccagtt tgcggtgcag gtgccctccg tcctcaagtt gctgggccgc
     7201 ttccgcccga cgctgtcgct ggcggcggag cccgtgcggc aggtgctgaa gaacttcggg
     7261 ccggtggtgc tggggcgcgg cgtggtccag ttcagcgcct gggtggacac ggccttcgcg
     7321 tcactcatct ccaatcgcgc gctgtcctcg ctgctctacg cgcagaccat ctacctcatc
     7381 ccggtgagcc tctttgggat ggcggtgtcc gcggcggagc tgccggagat gtcgcgcgcc
     7441 acgggggagg gcgccgacgt ggccgccagg ttgcgccaac gcattgacgg aggcgcacgc
     7501 cgcatctgct tctgggtcgt cccgtcggcg gcggccttcc tcttcctggg ggacatggtg
     7561 gcggcggcgt tgctgcagac ggggcgcttc gatgcggcgg actcgcgcta cctctggtac
     7621 ctgctcatgg gggcggcgct ggggttggtg gcctccacgg tgggccgcct ctatgcctcc
     7681 gccttctatg ctctgaagga tccgaagacg ccgctgcgct tcgcggtggt gcgcgtgctg
     7741 gtgggcaccc tgggcgcatg ggtgttgggc ctgcggctgc cgggctggct cggggtgccg
     7801 cagcacctgg gcgccgtggg cctcaccctg gccagtggcc tggtggcctg gctggaggcg
     7861 ggactgctgc gccgcaagct ggtgaagcag ctggggccgg tcggcgtccc gtctggcctg
     7921 ctgcctcgct tgtggggcgc cgcggtggtg gctggattgg tggcggtggg catcaaggtg
     7981 ggcctcacct cgctgctcgg gccgatgccc ggcgtgacgg cggagtgggg gggaggcttc
     8041 ctgatgccgc cgcgcctgca ccctgttctg ggcttcgcag cggtggcgct cccgttcggt
     8101 gctgtttatt tcgggatgtc agcggccctg ggcatcccgg aggcgggaac ggtgttgcgc
     8161 aaggtggggc ggaagctgcg tctggtgcgc taaccgccgg ggattgcttg gctctctgcc
     8221 cggcgagcgg ccgctatttg gtgttgcaat catcgggtcc gggatgctct gacgcggtgc
     8281 ctccgttatg gacggagcgt tggcgtcaaa gacatgtgcg tcatctggct tcctccggac
     8341 atggcgcgcg tgtagagtgc gccgcctttt tcaccggggc ccggcttctc gggaagccag
     8401 ggtcgaattc gcttcaggaa ggtctcgcag tgagcgacga gaagaacggt tccaattctg
     8461 gcgggatggg tcccaagaag ccgaaggcca ccttcggtga cgtgatgctc ggcatccctt
     8521 ccgggggagc tcgcagcgag ggcggtcggg gcgaacaacg tggcggtggc cgtggaggcg
     8581 agcgcggcgg cagtggccgt ggcgagcgcg aggcccagcc gcgtccggcg ggtggtgcgc
     8641 cgcgcgacga gcggggtccc cgtggcgggg gcgagcgccg tggcggaggc gaccgtcgtc
     8701 cgtcaggccc catggttgtc gtgaagcggg cgtcgggtgc catcgagacg cgcggcccgg
     8761 tgggggatgc tcccgccgag gcgaccgcga cggccgagga gacgaccgcc gccgccgagt
     8821 cttccgccgc gacgccggcc cccacgccgc gtccggtgac gccgacgccg gccccaacca
     8881 gcgcgctgta tgaggaagtc cccgaggccc agtccttcgc cgagatgttc gaggcgcagg
     8941 ccaaggaggg tggaacgccc agccgcaagg gcctgcgcgt gggcgagaag gtccgtggca
     9001 ccatcttcca gctcggcgcg gataccgcgt tcgtgtcggt ggaaggcgct gccaagagcg
     9061 aggcgatgat cgagcttcgc gagctgaagg acgatgaggg catcctgcgc ttcggcgtgg
     9121 gtgacaccat cgacgcgcac gtggtggagg tgggcgccaa gggcatcctc ctcagccgcg
     9181 cgctggccaa gggcagcgcg aacctggccc tgctggccga ggcccgcgct tccggcatgc
     9241 ccgtcgaggg actggtgctg agcgtgaaca agggcggtgt ggaggtcgcc atcggtgaca
     9301 tccgcgcgtt ctgccccatc agccagctgg acctgcgcta cgtggagaag ccggaccagt
     9361 tcatcggcga gaagctccag ttccgcgtga gcgaggtccg cgagcgcaac gtggtgctgt
     9421 cgcgccgcgc gctgttggag gacgagcagc gtcagctggc cgcggagacg cgcaagaacc
     9481 tggcccaggg caagatcgtc aagggcaagg tcaccggcgt gcgcgacttc ggcgtgttcg
     9541 tggacctggg cggcgtggag ggcatgattc ccgtctccga gctttcgtac acccgcgtcg
     9601 gtcacccgag cgacgtggtg aaggtcggtg acgacgtcga ggtcgaaatc ctccgcatgg
     9661 aggccggcca gcccaactcg ccggacaagt ccaagcagaa ggagcgcatc acgctgtcca
     9721 tgcgctcgcg ccaggaggat ccgttccaga aggcgctgtc cgaaatcaag gaaggcgacc
     9781 gcgtccaggg caaggtcgtc cgcctgcagc agttcggcgc cttcgtggag ctgcgtccgg
     9841 gcgtggacgg tctggtccac atctccgcgc tcagcgaccg ccgcatcgcg cacccgcgcg
     9901 acgtggtgaa ggaaggggag agcatctggg tgtcggtgga gaagatcgac acgcaggaga
     9961 agcgcatcgg cctgcgccgc atctccgagg aagaggcgca gcgtcctccc gaggagcgct
    10021 cggcgcccgc agcggaggcc gccgctccgg cgcagcccgc cgcgccccgt cccaaggtgg
    10081 gccaggtcgt cgtgggcaag gtggatcgca tcgagcccta cggcgtgttc ctcgcgttcc
    10141 cgggtggcaa gggcttgctg cccgcgagcg agacgggtac cgagcgtggc accgacctgc
    10201 gcaagcacta ctcgctgggc caggaggtga aggtggccat cctggacatc gacgcgtccg
    10261 ggaagatccg cctgtccgtc accgccgcca tccgcgcgga ggagcgcgcc gaggtggagg
    10321 cctggcagaa gacgcagcag ccgcagggcg cgggcaagaa gggcttcggc accttcgcgg
    10381 atctgctcag caaggcgcgc aagtaattcc ctgtctccag cgctgattcc gttgatgtgg
    10441 tcagcgctgg agaaaggtgt tgacggtaca ggcgagggac ggtacttacc gcctcgcctc
    10501 tctctgccgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgcag
    10561 gttcaaatcc tgcccccgca attcgttgta cttcgaaact tgattggaat cgcagtcagg
    10621 tttcgagtag ttgagcctca agacatgtcg cggggtggag cagcctggta gctcgtcggg
    10681 ctcataaccc gaaggtcgca ggttcaaatc ctgcccccgc aactcgttgt acctcgaatt
    10741 ctgattggaa tcgcagtcag gtttcgagtt gttgaaccca aacatgacgc ggggtggagc
    10801 agcctggtag ctcgtcgggc tcataacccg aaggtcgcag gttcaaatcc tgcccccgca
    10861 actc
//
