LOCUS       NZ_JALAYD010000028      6859 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain T16B1 contig_0000028, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAYD010000028
VERSION     NZ_JALAYD010000028.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6859)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205.44514070089042x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:51:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,297
            CDSs (total)                      :: 4,223
            Genes (coding)                    :: 4,080
            CDSs (with protein)               :: 4,080
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 89 of 143
            Pseudo Genes (incomplete)         :: 77 of 143
            Pseudo Genes (internal stop)      :: 28 of 143
            Pseudo Genes (multiple problems)  :: 42 of 143
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:20:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6859
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAYD010000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..1605)
                     /locus_tag="MOF39_RS21385"
                     /old_locus_tag="MOF39_21345"
     CDS             complement(<1..1605)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_Starter (3-300). E-value:
                     1.1e-89. Score: 292.7. Matches aSDomain:
                     nrpspksdomains_MOF39_RS21385_Condensation_Starter.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOF39_RS21385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_21345"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268288549.1"
                     /sec_met_domain="Condensation (E-value: 6.6e-76, bitscore:
                     243.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.7e-15, bitscore:
                     44.8, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQHTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVL
                     EGALNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQA
                     RIPFKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDP
                     LPDQPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSAS
                     RDTITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKA
                     EKEMLGMFVSSLPIRITVDPDADFLSFVRRVGREQLSVMRHQRFPYNLLVNELRKEQKD
                     LHNLIGISMQYQPLQWHNADGFDYETALYFSGYTATELSVQIQERTDTGTIQLNFDYQN
                     TLFSLEDIKRIQNHLLTLLENALQHPHSFIKELDMTNIREKQKLLYEFNKTEAVSPEAP
                     TLHGLFERQAAVTPERPAIRFSSDSLTYAELDMYASRLAARLAARGVTKESIVGVLSER
                     SPEMLIAVLAVLKAGGAY"
     source          1..6859
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="T16B1"
                     /submitter_seqid="contig_0000028"
     region          1..6859
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..6859
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6859
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1605](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1605)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(553..666)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21385_0005"
                     /evalue="9.60E-10"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="MOF39_RS21385"
                     /protein_end="351"
                     /protein_start="313"
                     /score="31.0"
                     /tool="antismash"
                     /translation="REQLSVMRHQRFPYNLLVNELRKEQKDLHNLIGISMQY"
     aSDomain        complement(706..1596)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF39_RS21385_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="1.10E-89"
                     /label="MOF39_RS21385_Condensation_Starter.1"
                     /locus_tag="MOF39_RS21385"
                     /protein_end="300"
                     /protein_start="3"
                     /score="292.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="HTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVLEGA
                     LNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQARIP
                     FKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDPLPD
                     QPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSASRDT
                     ITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKAEKE
                     MLGMFVSSLPIRITVD"
     CDS_motif       complement(718..807)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21385_0004"
                     /evalue="6.30E-09"
                     /label="C5_starter_265-294"
                     /locus_tag="MOF39_RS21385"
                     /protein_end="296"
                     /protein_start="266"
                     /score="28.3"
                     /tool="antismash"
                     /translation="DLAIGTYYGNRGSKAEKEMLGMFVSSLPIR"
     CDS_motif       complement(1141..1206)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21385_0003"
                     /evalue="5.00E-08"
                     /label="C3_starter_134-155"
                     /locus_tag="MOF39_RS21385"
                     /protein_end="155"
                     /protein_start="133"
                     /score="25.8"
                     /tool="antismash"
                     /translation="FHHIIMDGISLNVMGNQIIDLY"
     CDS_motif       complement(1411..1527)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21385_0002"
                     /evalue="3.40E-10"
                     /label="C2_starter_024-063"
                     /locus_tag="MOF39_RS21385"
                     /protein_end="65"
                     /protein_start="26"
                     /score="32.6"
                     /tool="antismash"
                     /translation="ICNLTACVKFRGNIDLDVLEGALNHSISRNDAIRFQLLE"
     CDS_motif       complement(1552..1590)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21385_0001"
                     /evalue="2.90E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="MOF39_RS21385"
                     /protein_end="18"
                     /protein_start="5"
                     /score="10.5"
                     /tool="antismash"
                     /translation="YSLTHAQRRVWFT"
     misc_feature    1805..1818
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.77"
                     /tool="antismash"
     gene            complement(1976..3454)
                     /gene="dacB"
                     /locus_tag="MOF39_RS21390"
                     /old_locus_tag="MOF39_21350"
     CDS             complement(1976..3454)
                     /EC_number="3.4.16.4"
                     /GO_function="GO:0004175 - endopeptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241941.1"
                     /locus_tag="MOF39_RS21390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_21350"
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="WP_268288550.1"
                     /transl_table=11
                     /translation="MKKSIKLYVAVLLLFVVASVPYMHQAAQAAEKQGALAGQIDKILA
                     DHPALEGAMAGITVRSAETGAVLYEHSGDIRMRPASSLKLLTAAASLSILGEDYSFTTE
                     VRTDGKFKGKKLNGNLYLIGKGDPTLLPSDFEEMAEKLKQSGVKEIKGNLIGDDTWYDD
                     IRLSPDMPWSDEYTYYGAQISALTASPNEDYDAGTVIVEITPGKKEGEKPAVSVSPKTD
                     YVTIKNDAETTAAGQKKDLTIEREHGTNTITIEGSVPVDASKTKEWIAVWEPAGYALDL
                     FKQALKKQGITVKGHVKTGAAPGSTDVLISHRSMPLSKLFVPFMKLSNNGHAEVLVKEM
                     GKVKKEEGSWEKGLEVLNNALPEFGVDPKSLVLRDGSGVSHINAVSSDQISQLLYDIQD
                     EKWFSAYLNSLPVAGNSDRMIGGTLRNRMKDTPAQGKVRAKTGSLSTVSSLSGYAETKS
                     GKTLVFSILLNGLIDDEDGKDIEDQIAVILANQS"
     gene            complement(3489..4466)
                     /gene="galM"
                     /locus_tag="MOF39_RS21395"
                     /old_locus_tag="MOF39_21355"
     CDS             complement(3489..4466)
                     /codon_start=1
                     /gene="galM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389718.1"
                     /locus_tag="MOF39_RS21395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_21355"
                     /product="aldose 1-epimerase"
                     /protein_id="WP_225516923.1"
                     /transl_table=11
                     /translation="MANDIEKITYLGTPAIKAGNEHIEMIVVPEWGSNVISLVDKTTQL
                     QLLREPETAESFHDTPTLYGIPILFPPNRISDGTFSFRGRTYHFDINEKDKHNHLHGFL
                     YQEKWNVVTMKQTDEEVIVETEIDLSELPHVYKQFPHHAVVRMTYTLKGNTLFKHATVM
                     NKGKEAFPWGIGYHTTFVFPEESSLFSLTADQQWELDERLLPTGKLMDVQNKEALHEGM
                     DLRSQQLDDVFLSSYQKRGGENQAVIYHQHAHISIIYKADEQFKHWVVYNADGKQGYLC
                     PEPYTWVTNAVNLNVSPSLTGVQVLEPGEETTAKSSITIELNHQ"
     gene            complement(4599..5990)
                     /locus_tag="MOF39_RS21400"
                     /old_locus_tag="MOF39_21360"
     CDS             complement(4599..5990)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389719.2"
                     /locus_tag="MOF39_RS21400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_21360"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_003241939.1"
                     /transl_table=11
                     /translation="METSQEQLVTQKKSNPSVWRSMSLFLVPLLLSNVLQSVGQLVGMM
                     AVGRWLGVDAVAAVSSFFPLFFLLISFTIGIGSGSSILIGQAYGAKNEERLKAVVGTTL
                     TFTFLLGVVLAVIGSIFTLDILRLMGTPENVIHVSASYARILFYAMPFMFLYFAYTTFL
                     RGTGDSKTPFYTLIVSTVINIALLPVLILGMFGFPKLGIYGSAYATVISTIATFIVLMV
                     YLRKRNHPLQFDKTVRRYLKMDKELLALLLRLGIPSSINMILVSLSEIAVISFVNHYGS
                     NATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANEFGRLKQVIRVGIWLNYIIGG
                     VLIILIYVFSHQILGLFLTEQETLYIAHRLLMITLWSYLLFGNAQIISATMRASGTVLW
                     PTVISIFAIWGVEVPVAFVLSHYTNLEILGVWVGYPAAFAVSLLLIYGYYQFVWKKKQI
                     TRLIQ"
     gene            6277..6822
                     /gene="iseA"
                     /locus_tag="MOF39_RS21405"
                     /old_locus_tag="MOF39_21365"
     CDS             6277..6822
                     /codon_start=1
                     /gene="iseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389720.2"
                     /locus_tag="MOF39_RS21405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_21365"
                     /product="DL-endopeptidase inhibitor IseA"
                     /protein_id="WP_268286778.1"
                     /transl_table=11
                     /translation="MKKYFLFLTTIALILSLSTNAFAKNTSGDLSQKQALQLALSAREH
                     FWNTMSGHNPKAKKAVCPSGTFEYQNLQYVYMCSDLGTKAKAVNYLTPIFTKTAIEKGF
                     KDYHFTVSKGKLAVPIGDGDNLLNWKKSTAKLISKKGGTVTYEFTVPTLDGSPAAKRKV
                     TFVKENNKWKVNQFDAVI"
ORIGIN
        1 atacgccccg cctgccttca atacagcaag taccgctatc agcatttcag gtgaccgttc
       61 agacaggaca ccgacgatgc tttccttcgt gacgccgcgt gccgcgaggc gcgcagccag
      121 ccggctggca tacatgtcaa gctcagcgta cgtcagcgaa tcgctggaaa agcggatggc
      181 tggccgctcc ggtgtgacag ccgcctgtcg ttcaaacaga ccgtgcaggg ttggggcttc
      241 aggcgaaaca gcttccgtct tattaaactc atacaacaat ttttgtttct ctcttatatt
      301 agtcatatct aattctttta tgaaactgtg cggatgttga agggcgtttt ccagtaacgt
      361 aagcagatgg ttctgtattc gcttaatatc ctcaagagaa aacaaggtat tttgatagtc
      421 gaaatttaat tgaatagttc cggtgtctgt ccgttcctgt atttgaacag aaagctcggt
      481 cgcggtgtat ccgctgaaat aaagcgccgt ttcatagtca aaaccgtctg cattgtgcca
      541 ttggagcggt tgatactgca tagatatacc gataaggttg tgtaaatcct tctgttcttt
      601 tcttaattca tttaccagca aattataagg aaaccgttgg tgacgcataa cggaaagctg
      661 ttccctgcct actctgcgga caaaggatag aaagtccgcg tcaggatcaa ccgttattct
      721 gatgggaaga gaactgacga acatgccgag catttctttt tcagcctttg accctctgtt
      781 gccatagtag gtgccgatgg caaggtcttt tttagaagtg atccggctga tgcatatgta
      841 aaatgaagcc ataaataagg aaatgatatt gatcttttgt tcttcgcaaa agattcgaat
      901 cgtttgccct agatgaggtg aaagggtgat ggtgtctctg gatgcggatg tgctctgttt
      961 ttgtaaagac gtctggccgg ctaacgagtg gtatcccggc ggattctgaa aggtttgagt
     1021 ccaaaataaa cggtcctttg taaaacgtga agactgtaaa tattgactct ctttttcgat
     1081 gtagctcaag taagaaggtt caggctgatc gggtaaagga tcttgctttt tcattttttg
     1141 ataaagatct ataatttgat ttcccatcac gtttaaggag atgccgtcca ttataatatg
     1201 atggaattta gcaaacagcc aaacctcatg attgtggatt ctaaggagat aaaattgata
     1261 aagaggcgag ttgaatagtt taaatgggat tctcgcttgt tcttgtatcc attgctctat
     1321 ttctatcatt tcaacatttg aaaaatctat tattcccagt gaagtatatt tatattcagt
     1381 aaggtacaat cgcggctcaa gctcttctcc ttctagcaac tgaaacctga tggcgtcgtt
     1441 gcgggagatg gaatgattca gcgcgccctc tagaacatca agatcaatat tgcctctgaa
     1501 tttcacacag gccgtaagat tgcaaatgct ggtacctggc tccagcagtt cggtaaacca
     1561 cactctcctt tgggcatggg ttaaagaata agtatgttgg ctcaacggat tccctccagt
     1621 tctcataata agaggataag aaaaaagtag aaaaatgaca ataaaagaaa ttatatgact
     1681 atataatcca tatatgcact atattaacgg atatttttta aagtacaata aaaaatatta
     1741 aatttttata acattttttt gaatatagta ttatttcttt agttttggtt tctttcttca
     1801 tcaaattttt taatataaag tgataaaagt atcattctga ttcgggtctt acacactaat
     1861 ttttcaattg tgctaatttg ctgttgcgcg taatctttct atttgttggc agatgcgacc
     1921 ggaatattat tgaagacata aaaaaacctg ttttccttgg aaaacaggtc tgacgctatg
     1981 attgatttgc caagatgaca gcgatttgat cctcgatatc ttttccatcc tcgtcatcaa
     2041 tcaatccgtt caaaagaata gaaaaaacaa gggtttttcc gctcttggtt tctgcgtacc
     2101 cggataaaga gctgactgtg cttaacgatc cggtttttgc ccttaccttt ccttgtgcag
     2161 gagtgtcttt catccggttc ctcagtgttc cgcctatcat cctgtcacta ttgcccgcaa
     2221 ccggcagaga atttagataa gctgagaacc atttctcatc ttgaatgtca tataacagct
     2281 gtgagatttg atctgaggat acagcattaa tatgggagac acctgatccg tctcttaaga
     2341 caagtgattt gggatcaaca ccaaattccg gaagcgcatt gtttaatact tcaagtccct
     2401 tttcccagct tccttcttct tttttcactt tccccatctc ttttacgagc acttcggcat
     2461 gtccgttatt gcttaatttc ataaacggca caaatagttt tgacagaggc attgagcgat
     2521 gagagattaa cacgtctgta gaaccgggcg cagcgcctgt cttgacgtgc ccttttacag
     2581 taattccttg tttttttagt gcctgcttaa ataaatccaa agcgtatcca gccggctccc
     2641 agacagcaat ccattccttt gtcttgcttg cgtcaaccgg tacgctccct tcaattgtga
     2701 tcgtgtttgt tccatgctct ctttcgatcg tgaggtcttt cttttggcca gccgcagtcg
     2761 tttcggcatc gttcttaatc gttacataat ccgtcttcgg agatacagaa acggcaggct
     2821 tttccccctc ttttttaccc ggggtgatct ctacaatcac agtgcctgca tcataatctt
     2881 cattcggaga cgctgttaaa gcggagattt gcgcgccgta atatgtgtat tcatcactcc
     2941 acggcatatc aggcgataat cggatgtcat cataccacgt atcatcaccg atcagattgc
     3001 ctttgatctc tttcacgccg gattgtttca atttctcagc catttcttca aaatcagatg
     3061 gtaaaagtgt cggatctcct ttccctatca aataaaggtt tccattcagc tttttccctt
     3121 taaatttgcc atccgtccgg acctcggttg taaaggaata gtcctctccg agaatagaaa
     3181 gtgatgcggc cgctgtcaaa agttttaaag aagaggcggg cctcattcgg atgtctcctg
     3241 aatgctcata tagaacagcg cctgtttccg cagaacgaac ggtgatgccg gccattgcgc
     3301 cttcgagtgc aggatgatca gctaaaatct tatcgatctg cccggcaagt gcaccttgtt
     3361 tttcagccgc ttgcgcagcc tgatgcatat aagggaccga agcaacgacg aaaagcagca
     3421 aaacagcaac ataaagcttt atgctttttt tcattttttt accccgcctc tctgcgcctc
     3481 atcattggct attgatgatt cagttcaatg gtgatactgc ttttggctgt cgtttcttct
     3541 cccggctcca gcacttgtac accagtgagt gatggagaca cattaagatt cacagcgttt
     3601 gtcacccatg tgtaaggttc agggcataaa tatccctgct ttccgtccgc gttgtataca
     3661 acccaatgtt taaattgttc atctgcctta taaataatgc ttatatgtgc atgctgatga
     3721 taaatgactg cctgattttc tccgcccctt ttctgataag aagataaaaa gacatcatcc
     3781 agctgctggc tgcgtaaatc catcccttca tgcaaggctt ctttattctg tacatccatc
     3841 agtttcccgg tcggcagcag gcgctcatcc aactcccatt gctgatctgc tgtcagggag
     3901 aataacgagc tttcttctgg aaatacaaat gtagtatgat agccgattcc ccaaggaaac
     3961 gcttcctttc ctttgttcat gaccgtcgca tgtttaaata gagtattccc ctttagcgtg
     4021 tatgtcatcc gaacgacggc atgatgcgga aattgcttat acacatgggg aagctctgaa
     4081 aggtcaattt cagtttcaac gatcacttct tcatcagttt gcttcatggt cactacgttc
     4141 catttttcct gataaagaaa cccatgaaga tggttgtgtt tatccttttc attgatatca
     4201 aaatgatatg ttctgccccg gaaactgaat gtaccgtcac ttatccggtt aggcggaaac
     4261 aggatcggaa tgccatacaa tgttggtgta tcatgaaagc tttctgctgt ttccggttca
     4321 cgcaaaagct gcagctgtgt cgttttgtcc acaagagaaa tgacgttgct cccccactcg
     4381 ggaacgacaa tcatttctat atgttcgttg cctgctttga tggcaggtgt tcctaaatac
     4441 gtaatcttct caatatcgtt tgccatgtgt actcaacctc tccttgattc attgcttgta
     4501 taagaattca atgtaatgtg ccatatttcc tccatttatg ttttctcttt atcaaacaaa
     4561 aaacaccgtt caattgttga acggtgtgat tcgggatgct attggatgag acgtgtaatc
     4621 tgtttctttt tccaaacgaa ttgataatac ccataaatca acagcaagct gacggcaaac
     4681 gcagccggat agccaaccca gacgccgagt atttcaagat ttgtgtagtg ggaaagtacg
     4741 aacgcgacag gcacctctac tccccagatg gcaaaaatac tgataacggt cggccataaa
     4801 accgttccgc ttgcccgcat cgtcgcagaa ataatttggg catttccaaa gagtaagtaa
     4861 ctccacaatg taatcatcaa gagacgatgc gcaatgtaaa gcgtctcttg ttctgttaaa
     4921 aataatccta aaatctgatg tgaaaaaaca tagatcagaa tgatcagcac accgccaatg
     4981 atataattca gccaaatccc gacacgaatt acctgtttta ggcggccaaa ctcgttcgct
     5041 ccgatcgatt gcgcggcgaa tatagacacc gcaataccaa ggctgacggc cggcatctgc
     5101 acatagctgg ctacctggtt gaccacgccg tatgcagctg tcgcattaga gccataatgg
     5161 ttgacgaatg aaataaccgc tatctctgat aatgagacga gaatcatatt gatgcttgat
     5221 ggaatcccaa ggcgcagcag gagagccagc agttctttat ccattttcaa ataacggcgc
     5281 actgttttat caaattgcag cggatggttt cgttttcgca aatacaccat taacacaata
     5341 aaagtcgcaa tcgtggaaat gacggttgca tatgcggagc cgtatatgcc cagtttcgga
     5401 aaaccgaaca tgccaaggat caagacaggc aaaagcgcta tgttaatgac cgtgctcaca
     5461 atcagcgtat aaaaaggtgt cttagaatcc ccagttccgc gcaaaaatgt tgtataagca
     5521 aaatataaaa acataaacgg catcgcgtaa aataaaatac gtgcatagct ggcgctgaca
     5581 tgaattacat tttcaggcgt gcccattaaa cgaagaatat ccaaggtgaa aatactgccg
     5641 atgacggcta acacaacccc taataaaaac gtaaacgtga gcgtcgtccc gacaacagcc
     5701 tttaaccgct cctcattttt cgccccgtag gcttgtccga tcaggatcga gcttcctgaa
     5761 ccgatcccaa ttgtaaatga gattaaaaga aaaaataacg ggaaaaagga tgatacagct
     5821 gcaaccgcat caacaccaag ccatctgcct acagccatca tcccgactag ctggccgact
     5881 gattgcagta cattgcttag cagaagcgga actaaaaaga gtgacataga ccgccagaca
     5941 gatggattgc ttttcttctg agttacaagt tgttcttgtg atgtttccat attgaaagct
     6001 cctttatttg aattcgtttg taaacatgac cctattcttt tgacaaaaac aggatatgta
     6061 acatgttatt attcatcact gtacatatga aacccttccc gattcttcta attttacaca
     6121 tctttttagc ttatgaagaa taaactagca agtaacagcc tattttcttg tgagtaaaag
     6181 aataaaaaat aatttatgca aaagtattgt aatctatccg taattattgt aacatttgta
     6241 acataagaga aagagatttt gaaggagtga accacaatga aaaaatattt tctatttcta
     6301 acaactattg cacttattct gtcattaagc acaaatgcat ttgcgaaaaa tacttcaggt
     6361 gatttatcac aaaaacaagc actgcaactg gcgttatcag caagagagca tttttggaac
     6421 acaatgagcg gccacaatcc aaaagcgaaa aaagcagttt gcccatcagg cacatttgag
     6481 tatcaaaatc ttcaatacgt gtacatgtgc agtgacctag gcactaaagc aaaagcagtg
     6541 aattacttaa ctcctatttt cacaaaaaca gcaattgaaa aaggctttaa agactatcat
     6601 ttcactgttt caaaaggaaa acttgccgtg ccaatcggcg acggtgacaa ccttttaaat
     6661 tggaaaaaat caactgctaa actgatctct aaaaaaggcg gcacagtcac atacgaattt
     6721 accgttccta ctttagacgg atcacctgca gcgaaacgga aagtgacatt tgtaaaagaa
     6781 aataataaat ggaaagtcaa tcaatttgac gcagttatat aataaaaaac gccctgcata
     6841 agggactgac ccccgtttt
//
