LOCUS       NZ_JALAYD010000027      1709 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain T16B1 contig_0000027, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAYD010000027
VERSION     NZ_JALAYD010000027.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205.44514070089042x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:51:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,297
            CDSs (total)                      :: 4,223
            Genes (coding)                    :: 4,080
            CDSs (with protein)               :: 4,080
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 89 of 143
            Pseudo Genes (incomplete)         :: 77 of 143
            Pseudo Genes (internal stop)      :: 28 of 143
            Pseudo Genes (multiple problems)  :: 42 of 143
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:20:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1709
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAYD010000027.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS_motif       complement(<1..74)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21380_0005"
                     /evalue="9.10E-09"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOF39_RS21380"
                     /protein_end="570"
                     /protein_start="545"
                     /score="27.7"
                     /tool="antismash"
                     /translation="YNMPAVLELEGKLDPERLDRAFKEL"
     source          1..1709
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="T16B1"
                     /submitter_seqid="contig_0000027"
     gene            complement(<1..>1709)
                     /locus_tag="MOF39_RS21380"
                     /old_locus_tag="MOF39_21340"
     region          1..1709
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1709
                     /SMILES="NC(CCC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1709
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1709](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1709)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..1709)
                     /NRPS_PKS="Domain: AMP-binding (0-336). E-value: 8.1e-90.
                     Score: 293.3. Matches aSDomain:
                     nrpspksdomains_MOF39_RS21380_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (438-507). E-value: 9.1e-27. Score:
                     85.1. Matches aSDomain: nrpspksdomains_MOF39_RS21380_PCP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOF39_RS21380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_21340"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_268288548.1"
                     /sec_met_domain="AMP-binding (E-value: 5.8e-108, bitscore:
                     347.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.7e-18, bitscore:
                     53.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 6.5e-16, bitscore:
                     44.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKAGGAYLPLDPAYPKERLSYMLKDSGAALLLAQPGCSAPSFSGE
                     MLEVDMASLASEKAENDVFTPADGCSLAYVIYTSGSTGKPKGVAVEHRQAVFFLTGMQR
                     QFPLQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAIHQEKVT
                     TAHFIPAMLNSFLDQAEMKALGDGTSLKRVFAGGEPLAPHVAARFASLLPQVSLIHGYG
                     PTEATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDAHLAVQPCGVAGELYIAGAG
                     VARGYLNRPALTEERFLEDPFYPDERMYKTGDLARWLPDGNIEFLGRTDDQVKIRGYRI
                     EPGEIEAVLRGIEGIREAAVTVRTDSGEPELCAYAEGLRRNEVRTQLERLLPGYMIPAH
                     IIEMERWPVTPSGKLDRNALPAPDGAADEETYTAPRNVTEMKLAQLWEDVLKNGPVGIH
                     DNFFDRGGHSLKATALVSRIAKEFDVQVPLKDVFAHPTVEGLASVIREGTDSPYEAMKP
                     TEQREAYPVSSAQKRIYVLQQLEDGGTGYNMPAVLELEGKLDPERLDRAFKEL"
     aSDomain        complement(189..395)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF39_RS21380_PCP.1"
                     /evalue="9.10E-27"
                     /label="MOF39_RS21380_PCP.1"
                     /locus_tag="MOF39_RS21380"
                     /protein_end="507"
                     /protein_start="438"
                     /score="85.1"
                     /tool="antismash"
                     /translation="EMKLAQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFD
                     VQVPLKDVFAHPTVEGLASVIREG"
     aSModule        complement(189..1709)
                     /complete
                     /domains="nrpspksdomains_MOF39_RS21380_AMP-binding.1"
                     /domains="nrpspksdomains_MOF39_RS21380_PCP.1"
                     /locus_tags="MOF39_RS21380"
                     /monomer_pairings="Glu -> Glu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(672..737)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21380_0004"
                     /evalue="2.80E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="MOF39_RS21380"
                     /protein_end="346"
                     /protein_start="324"
                     /score="41.6"
                     /tool="antismash"
                     /translation="FLGRTDDQVKIRGYRIEPGEIE"
     aSDomain        complement(702..1709)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF39_RS21380_AMP-binding.1"
                     /evalue="8.10E-90"
                     /label="MOF39_RS21380_AMP-binding.1"
                     /locus_tag="MOF39_RS21380"
                     /protein_end="336"
                     /protein_start="0"
                     /score="293.3"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="MKAGGAYLPLDPAYPKERLSYMLKDSGAALLLAQPGCSAPSFSGE
                     MLEVDMASLASEKAENDVFTPADGCSLAYVIYTSGSTGKPKGVAVEHRQAVFFLTGMQR
                     QFPLQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAIHQEKVT
                     TAHFIPAMLNSFLDQAEMKALGDGTSLKRVFAGGEPLAPHVAARFASLLPQVSLIHGYG
                     PTEATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDAHLAVQPCGVAGELYIAGAG
                     VARGYLNRPALTEERFLEDPFYPDERMYKTGDLARWLPDGNIEFLGRTDDQVKIR"
     CDS_motif       complement(819..908)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21380_0003"
                     /evalue="3.90E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="MOF39_RS21380"
                     /protein_end="297"
                     /protein_start="267"
                     /score="57.5"
                     /tool="antismash"
                     /translation="PCGVAGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       complement(1434..1493)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21380_0002"
                     /evalue="1.40E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOF39_RS21380"
                     /protein_end="92"
                     /protein_start="72"
                     /score="36.0"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVAVEH"
     CDS_motif       complement(1677..1709)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF39_RS21380_0001"
                     /evalue="8.30E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="MOF39_RS21380"
                     /protein_end="11"
                     /protein_start="0"
                     /score="12.9"
                     /tool="antismash"
                     /translation="MKAGGAYLPLD"
ORIGIN
        1 agctctttaa aagccctgtc cagtctttcg gggtccagct tcccttccag ctctaacaca
       61 gccggcatat tgtagcctgt ccctccgtcc tccagctgct gaaggacata gatccgtttt
      121 tgcgcggagg aaaccggata ggcctcacgc tgttccgtcg gtttcatcgc ttcatatgga
      181 ctgtcagttc cttcacggat aacggaagcc agtccttcaa cagtcggatg ggcaaacaca
      241 tctttcagcg gcacctgtac atcgaattct ttggcgatcc gggacacaag cgccgttgct
      301 tttaaggaat gtcctccgcg gtcgaaaaag ttgtcgtgaa tcccgacagg gccatttttc
      361 agcacgtcct cccacagctg ggcaagcttc atctctgtca catttctcgg cgccgtgtag
      421 gtctcctcat ctgccgctcc gtcaggagct ggcagggcgt ttcggtcaag ctttccgctt
      481 ggcgtaaccg gccaccgttc catctctatt atatgggctg ggatcatgta gcctggcagc
      541 agtctttcaa gctgcgtccg cacttcgttt cttcggagtc cttctgcata ggcgcataat
      601 tccggctcgc cgctgtctgt ccgtacggta acggctgcct ccctgatgcc ctctatgcct
      661 cttagcaccg cttcaatttc tccgggctca atccggtagc cgcggatttt cacctgatca
      721 tccgtgcggc cgaggaattc aatattcccg tcagggagcc agcgcgccaa atccccggtt
      781 ttgtacatac gttcatccgg gtaaaacggg tcctcaagaa aacgctcctc cgttaaggcc
      841 ggccgattca aatagcctct ggccacaccc gctccggcta tgtacagttc gccggcgaca
      901 ccgcaaggct gtacggctaa atgcgcatct aaaacataca aacgcgcacc cggtacgggc
      961 ttcccgatcg gaatccgcaa gcgatccctg tcccgctctg ggtccaacac gtaaaatgcc
     1021 gcgtcaaccg ttgcttctgt cggcccgtag ccatgaatca gcgatacctg cggcaataaa
     1081 gaagcaaaac gggctgccac atggggcgca agcggttcac ctccggcgaa tacacgcttc
     1141 aggcttgtcc catcaccgag cgctttcatt tcagcttgat cgagaaagct gttcagcata
     1201 gccggaataa aatgagccgt tgtcactttt tcctgatgaa tggcttttac aatcaatgcc
     1261 gggtctttct cccagccggg cggaagcaga taagccgaag cgccggaaag ggaccaccaa
     1321 aacagctgcc acacagacgc atcaaaggaa aaagaggttt tcaccataac aatgtcatct
     1381 tcctgaagcg gaaactgacg ctgcatgcca gtcagaaaga aaacagcctg gcggtgctcg
     1441 acggccactc cttttggctt cccggtggag cccgatgtat agatgacata ggcgagagaa
     1501 cagccgtcag caggagtaaa cacatcgttt tctgcttttt cgctcgcaag agatgccatg
     1561 tccacttcaa gcatctctcc ggaaaagctc ggtgcggaac atcccggctg tgccagcaag
     1621 agcgcggccc cgctgtcttt cagcatgtag ctcagccgct cctttggata tgcaggatca
     1681 agcggcaaat acgccccgcc tgccttcaa
//
