LOCUS       NZ_JALAYD010000016     12956 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain T16B1 contig_0000016, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAYD010000016
VERSION     NZ_JALAYD010000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 261263)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 261263)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205.44514070089042x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:51:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,297
            CDSs (total)                      :: 4,223
            Genes (coding)                    :: 4,080
            CDSs (with protein)               :: 4,080
            Genes (RNA)                       :: 74
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 89 of 143
            Pseudo Genes (incomplete)         :: 77 of 143
            Pseudo Genes (internal stop)      :: 28 of 143
            Pseudo Genes (multiple problems)  :: 42 of 143
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:20:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 248307
            Orig. end                         :: 261263
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAYD010000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12956
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..12956
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12956
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[10000:10747](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10747)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            complement(426..1358)
                     /gene="hprK"
                     /locus_tag="MOF39_RS18560"
                     /old_locus_tag="MOF39_18525"
     CDS             complement(426..1358)
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="MOF39_RS18560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18525"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_003235406.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGVNRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEDKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQDE"
     misc_feature    1470..1485
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            1540..2730
                     /gene="nagA"
                     /locus_tag="MOF39_RS18565"
                     /old_locus_tag="MOF39_18530"
     CDS             1540..2730
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="MOF39_RS18565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18530"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_148982516.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.6e-42, bitscore:
                     139.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKGIAIVTENEVIKNGYVGINGGKISTVSTERPKESYSK
                     EIQAPADSVLLPGMIDIHIHGGYGADTMDASFSALDTMSSRLPEEGTTSFLATTITQEH
                     GHISQALVNARDWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIRYLKDESIIASMGHTDADSALLSEAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGKTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTEGKDADLVIVSSDCKVILTICRGNIAFISKEADQI"
     gene            2727..3455
                     /gene="nagB"
                     /locus_tag="MOF39_RS18570"
                     /old_locus_tag="MOF39_18535"
     CDS             2727..3455
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="MOF39_RS18570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18535"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_268284564.1"
                     /transl_table=11
                     /translation="MKVVECQTYEELSQKAARIAADTIKEKPDAVLGLATGGTPEGTYR
                     ELIRLHQTENLSFKNVTTVNLDEYAGLSSDDPNSYHYYMNSRFFQHIDSQPSRHFIPNG
                     NADDLEAECRRYEQLVESIGGTDIQLLGIGRNGHIGFNEPGTPFKSRTHVVTLNEQTRQ
                     ANARYFSSIDSVPKKALTMGIQTILSSKRILLLISGKSKAEAARKLLEGDVSEDFPASA
                     LHLHPDVTILIDRKAAALRP"
     gene            3473..4204
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="MOF39_RS18575"
                     /old_locus_tag="MOF39_18540"
     CDS             3473..4204
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="MOF39_RS18575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18540"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_003235409.1"
                     /transl_table=11
                     /translation="MNIDKQSPIPIYYQIMEQLKAQIKSGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGFLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIESTEELAALLGCGHPSPIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYEHIEKYNSIPISRAKQELEPSAATSKEASILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(4223..8092)
                     /locus_tag="MOF39_RS18580"
                     /old_locus_tag="MOF39_18545"
     CDS             complement(4223..8092)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="MOF39_RS18580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18545"
                     /product="metallophosphoesterase"
                     /protein_id="WP_268288452.1"
                     /transl_table=11
                     /translation="MRKYTVIASLFLSFLSVLSDGHHESKAFPVIKQEPFQTPHYIPLL
                     ENPPQIDVKAEMKSNLMIEAQVRDNEYQAFSAFLFYKQSNELGYKMVPMDPTPGAIHKF
                     LAQIPKRLIWNSELEYYIVISNGKQRVESETKKLKLEGYQADLAHIPELLITELAVDTK
                     NTGRADGYEFIEIYNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERITIPSGETFVV
                     WLKPVGHPELTAEDFNRYYQTQLTEGKNLAVLEETEGMANTRPRSVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLDGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILTEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKSHGLRMNIENRDTLSGTQ
                     FLKATTDGRTNSIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPELLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRVSPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLKWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEAEASLDGESI
                     TLPYHTSSSELLPGKHSLKITATDLAGNKTVIERTFKTEREHPDQPEIIDSEADTHRAK
                     LSVRVKDPTDDPMDVAFYRGFQYTARNNVKIFKHASLTEPPKSFVPENETPFTNKELER
                     VSAVDGKTVSTEKKELFPYHRFEVTVDPSVDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDHKPFTLKASVIASDFVRKSKMNVIVQDEIPPAKDMYTFAWMSDTQY
                     YAESYPHIFDKQTAWIKSNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSHRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDEDIAWMNQVLKKHPDRMAVLAFHEYLLVSGNRSPIGEKIFKKVVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPHLQDKNYYDTETYGNKDEFSLSLDLKPRVKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDDLEPHSVYYWYTIVEDSFNGKTKSPIWKFKTKKETYRPSPDQFDFR
                     HVSNP"
     gene            8257..8730
                     /locus_tag="MOF39_RS18585"
                     /old_locus_tag="MOF39_18550"
     CDS             8257..8730
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="MOF39_RS18585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18550"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_225514489.1"
                     /transl_table=11
                     /translation="MDNSIHDELFQAIQQFALKRDKRVWQKVQIPSHSMSSAHLEHLKK
                     DWTLTQLHIVSCIHTSQNVNNSFLASRLHISKAAVSKAVQALLKHNIITVTKKPGNKKE
                     IFYTLTESGRELAALHEQLHEKAKEQYKQLFNEFSIDDLKTVTAFFNLWVKHM"
     gene            complement(8766..9986)
                     /gene="cypX"
                     /locus_tag="MOF39_RS18590"
                     /old_locus_tag="MOF39_18555"
     CDS             complement(8766..9986)
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010332029.1"
                     /locus_tag="MOF39_RS18590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18555"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_003235412.1"
                     /sec_met_domain="p450 (E-value: 6.1e-38, bitscore: 124.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQSIKLFSVLSDQFQENPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFVGDALDHL
                     SPFIKQNAENLLDPYLEKGRIDLVNDFGKTFAVCVTMDMLGLDKRDHEKIAEWHSGVAD
                     FITSIFQTPEARAHSLWCSEQLSDYLMPVIEERRVNPGSDLISILCTSEYEGMAMSDKD
                     ILALILNVLLAATEPADKTLALMVYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEHPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKTEIEIVANIVLDKMRNIRLEEGFRYAESGLYTRGPVSLH
                     IAFDRAQ"
     gene            complement(10001..10747)
                     /gene="pchC"
                     /locus_tag="MOF39_RS18595"
                     /old_locus_tag="MOF39_18560"
     CDS             complement(10001..10747)
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="MOF39_RS18595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18560"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_268278123.1"
                     /sec_met_domain="CDPS (E-value: 4.8e-83, bitscore: 272.1,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEMVTERKPVDFTAESLTQNCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWATAEFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     AAHGGDSKAIHTFSDFAENNAYQLMRQEVEHAFFEQPHFRHACLDMSREAMIGRARGIG
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIR
                     MRPNQGYLIVQETAQMLSETRITSEG"
     gene            11177..11686
                     /gene="pchR"
                     /locus_tag="MOF39_RS18600"
                     /old_locus_tag="MOF39_18565"
     CDS             11177..11686
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="MOF39_RS18600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18565"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_268288453.1"
                     /transl_table=11
                     /translation="MPDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVIDIPK
                     NMTSIHVIDCIGQHEPINNAGIAKKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGKRIFDLHEKLHKKKELAFYQFLDSFSQDEQNAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     gene            11708..12919
                     /locus_tag="MOF39_RS18605"
                     /old_locus_tag="MOF39_18570"
     CDS             11708..12919
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="MOF39_RS18605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF39_18570"
                     /product="MFS transporter"
                     /protein_id="WP_268278124.1"
                     /transl_table=11
                     /translation="MAQMKSKAVLILYIVCFSAFFASLSQNIYSPIIPIIKESFHVSAA
                     MVNLSVSVFMIVTAIMQIILGTIIDFKGARFVLIAGILTTTAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGSERGSAMGTYQMLLSAAPAVAPVLGGFIGGAAGY
                     EGIFWMLTAISVVMLVANSMIFPKDSPAGSKQKTKGNVFTHYKSIFTNRTGNVILALSF
                     VLFFIYFAVIVYLPILLTEHYHIDVSIAGLLYLPLALSTIAGTFLFKRIQAKISLNTLF
                     IGSNAVAACSIMLFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GIFNFIRYTGMAAGPMISAYLLTMMPSAMSFGLLGLVFAALSFCLLPQMYPPQKRVKQK
                     KHHM"
ORIGIN
        1 agcggccaat cgcctggccg agcaaaatgc ttggcgcggc aatatccgca agcttccaaa
       61 acgaaaggtt tttcactctt gaaaacacat aacctgttaa aatggcgccg attaacccgc
      121 cgtgaatggc aatgccgccc ttccaaatct taatgatttc tcccggatgt gccgcataat
      181 aatcccattc aaatgcaacg taataaattc gtgcgcaaat aatcgcaatc ggaatggcaa
      241 acaggacaag atcgataaac gtgtctttct gcagcccccg tttctcactt tccctcatcg
      301 caatccaaag ccctagcaaa gcgccgagac cgataatgat tccgtaccaa tggacggcta
      361 acggccccag ctgaaaagct atcggattga gtggttctat cgcttcattc atttaacgcc
      421 aactcctatt cgtcttgttc gccgtcttca atgacgtccg ccagcttgtt ggtaaattgc
      481 tcagcggcgt tcagacccat ccgcttcaat ctgaagttca tcgctgccac ctcgatgatg
      541 actgcgagat ttcggcccgg acggacggga atcgtcagtt ttgtaatttc tgtatcaatg
      601 attttcatgg tttcttcctc aagtccgaga cggtcgtatt gcttgccctg ctcccagagt
      661 tcaagattca tgacaatcgt aattcgttta ttgcttctga ccgcgccggc accaaacagg
      721 gtcatcacgt tgataatgcc aagccccctg atttcaagga gatgctcaat taattcaggc
      781 gcgtttccga caagggtatc ctgatcctcc tgacgaattt caacgcaatc atcggcaacg
      841 aggcggtgcc cccttttcac aagctccagt gcggtttcac ttttcccgac gccgcttttt
      901 cccgtaatca gcacgccgac accgtaaata tcgacaagta cgccatgaat cgctgtagta
      961 ggcgccagac ggctctcaag aaaattggtt aaacggcttg acagccttgt cgttttcagc
     1021 ggcgatctga ggacaggcac gccgtttttc tcggaagcgt caatcagctc ctgcgggata
     1081 ggcatatctc tggaaagaat aatagctggt gttacatcag tgcacagaga atccattcgc
     1141 tgctttttat cctcttccgg cagctgttca aagaaagaaa gctctgtttt tccgagaagc
     1201 tgcacgcgct ctctcggata atatgtaaaa tatccggcaa tttcaatgcc tggtcttgat
     1261 aagtcactca ttgtaatcgg acggttaacc ccttcttctc cgctgattaa ttccaaattg
     1321 aactgttcca ttacgtcttt tgtgcgaacc tttgccacga tatgttcctc ctgttccggg
     1381 ctgccccgag cttgctcaca atactttcat tttatcactt tcgggcttga acctaaaaca
     1441 gattttataa aagacgggaa aacacctcag ctggtctaga tcactagtct gaaaaggggt
     1501 aaaataaatg tattcatatt ccagaaaggc ggatcaccta tggcagaaag tcttcttatc
     1561 aaaggcatcg cgatcgtgac agaaaatgaa gtaatcaaaa atggctatgt cggaatcaat
     1621 ggcggaaaaa tcagcacagt ttcaacagaa cggcctaaag agtcttactc aaaagagatt
     1681 caagcaccgg cagattccgt tttgcttccc gggatgattg acattcatat tcacggcggc
     1741 tatggcgcag atacgatgga tgcaagcttt tccgccctcg acaccatgtc ctcaagactt
     1801 ccagaagaag gcacgacgag ctttttagca acaaccatta cccaggaaca tgggcacatt
     1861 tcccaagctt tggtgaatgc aagagattgg aaagcagctg aagaatcctc tttattaggc
     1921 gctgaactgc tcggcattca tttggagggg ccctttgttt cacctaaaag agccggagcg
     1981 cagccaaagg aatggatcag gccttctgat gttgcgcttt tcaaaaagtg gcagcaggag
     2041 gcgggcggac tgatcaaaat cgtcacactt gcgccggagg aggatcagca ttttgaactg
     2101 atccgctatt tgaaagacga atcgattatc gcgtcgatgg ggcacacaga tgccgactcc
     2161 gcattactgt cggaggccgc gaaagcaggc gcgagccata tgacccatct ttacaacgcg
     2221 atgagcccgt ttcatcaccg agagcccgga gtgatcggaa cggcgctagc ccatgatggg
     2281 tttgtgacag agcttatcgc tgacggcatc cactcccatc ctttagcggc aaagcttgct
     2341 tttttggcaa aaggcagcag caagctcatt ttaattaccg attccatgag ggcaaaaggc
     2401 ctgaaagatg gtgtatacga gttcggcggc caaagtgtga cggtaagagg aaagacagct
     2461 cttctttccg acggaacact tgccgggtcg atcctcaaaa tgaacgaagg cgcacggcat
     2521 atgcgtgagt ttacaaattg ctcttggacg gatatagcta atataacttc tgcaaacgca
     2581 gccaaacagc tgggtatctt tgaccgaaaa ggcagtgtga cagagggaaa agatgcggat
     2641 cttgtgattg ttagcagtga ttgcaaggtg attctcacca tctgccgcgg aaacattgca
     2701 tttatatcca aggaggctga ccagatatga aagtagttga atgtcaaaca tatgaagagc
     2761 taagccaaaa agcagccaga atagccgcag acacaatcaa ggaaaaacct gacgccgttc
     2821 tcggtttagc aacaggcggc acaccggaag gcacgtatcg cgagctgatc cgcctgcacc
     2881 aaactgagaa tctttcattt aaaaacgtca ccacggttaa tttggatgaa tacgccggac
     2941 tttcaagcga tgatccgaac agctatcact actatatgaa ttctcgtttt tttcagcata
     3001 tcgacagcca gccaagccgg cattttattc ccaatggaaa tgcagacgac ctggaagccg
     3061 aatgcaggcg gtacgaacaa ctagtcgaat ccattggcgg cactgacatt cagcttctcg
     3121 gcatcggccg aaacggacat atcggcttca acgaaccggg aacgcctttc aagtcgcgaa
     3181 cacacgttgt gacattaaat gaacaaaccc gccaggcgaa tgccagatat ttttcttcaa
     3241 tagacagtgt gccgaaaaaa gcactcacaa tgggaatcca gacgattctc tcaagcaagc
     3301 gcattctgct gcttatttcc ggcaaaagca aagcggaagc tgcgagaaag ctgttagaag
     3361 gagacgtaag cgaagatttc ccagcatcgg ctttgcacct gcaccccgat gttacgattt
     3421 tgattgatcg taaagccgcg gcattaagac cttgaaagga acatgctgac ttatgaatat
     3481 cgataaacaa tcgcctattc cgatttatta tcagattatg gagcaattaa aagcccaaat
     3541 taagagcgga gagctgcagc cagatatgcc tcttccctcc gagcgtgaat atgccgaaca
     3601 attcggaatc agccggatga cggttcgcca ggcgctttct aatttagtca atgaaggctt
     3661 tctttatcgc ctgaaaggac ggggcacctt tgtcagcaag ccaaaaatgg aacaagcact
     3721 ccaagggctt acaagcttta ccgaggatat gaaaagccgc gggatgacac cgggcagcag
     3781 gctcattgac tatcagctta ttgagtcaac tgaggagctc gcagctttat taggctgcgg
     3841 gcatccctct cctatccata aaatcactcg agtgcggctg gcaaatgata ttccgatggc
     3901 aattgaatcc tcgcatattc cgttcgagct tgcgggtgaa ttaaacgaat cgcattttca
     3961 gtcgtcgatt tacgagcata ttgaaaagta caacagcatc ccgatttccc gtgcaaaaca
     4021 ggagcttgaa ccaagcgctg caacatcgaa agaagcaagc attcttggca ttcaaaaagg
     4081 ggcgcctgtc ctgttaatca aacgaacaac gtatttacag aacggaactg cttttgaaca
     4141 tgcaaaatca gtatacagag gcgaccgtta tacatttgtc cactatatgg accggctttc
     4201 ataaaaaaat ctccaaccct ttttaaggat tggagacatg gcgaaaatca aactggtctg
     4261 gcgacggacg atatgtctct tttttcgtct tgaacttcca gatcggtgat ttcgttttgc
     4321 cgttaaagct gtcttccaca atggtgtacc aataataaac agagtgcggt tcaagatcat
     4381 cccagcggaa ttcagcggta tccccgctct tcacttgctc ccgttttccg agctcttcat
     4441 tggtatatac attacattca aaataatcgg tttccacctt tttgaccctc ggcttcaaat
     4501 caagcgaaag cgaaaactca tccttgttgc cgtaggtttc cgtatcatag tagtttttat
     4561 cttgtaaatg cggagagtac gtcgacacat gcaccatatc attcgcctgg tcaaactgca
     4621 gcaaacgcaa atacccttgt ccgccttctg gcccgccctg ataatcggcc agcatttgat
     4681 gcacgaggcg atccggtttt ccatcaccat catcatccag ctcatcagtt tttctcatcg
     4741 cactatgata atgcccgctt aacaccatca ccacattcgg attcggcttc acgacttttt
     4801 tgaatatctt ctctccgatc gggcttctgt ttccgctgac cagcaaatac tcatgaaaag
     4861 ctaaaacagc catccggtca ggatgcttct tcagcacttg attcatccac gcgatgtctt
     4921 catccgtaat gccccatccc atgtacagca tgatataatc attgccgttg ctcgaaatta
     4981 agtcataatg tccgcggtta tttttataag aaccgccata atgcaatttt ttatcaaaac
     5041 gatgactgcc aaagtatttg ccaaacgccc tgtaggagcc gtccttatgc ccgacatcgt
     5101 ggttacctgc cagcacgccg taaggaatcc cgcttttatc cagaacagac attgaccgat
     5161 cagcgttttt ccactgtctg atatcggcag aatcatctac aatatcaccc gtgtgaaaca
     5221 catatttgat attgagctgc ttttgattgc ttttgatcca tgcggtctgt ttatcaaaaa
     5281 tatgcggata gctttccgcg taatactgtg tatcggacat ccaggcaaat gtgtacatat
     5341 ccttcgcagg aggaatttca tcctggacaa tcacattcat cttcgacttc cgcacaaaat
     5401 cggaggcgat tactgacgcc ttcagcgtaa acggtttgtg atctttggca acgaagctgt
     5461 caaccggctg ccactcgttt gtccggtaat tccaagcata catcgtcacc tttcttcccg
     5521 gcaaagaact tcccttccag accgcttccg ccaagtcatt ctcatcaact gacggatcaa
     5581 ccgtcacttc aaagcgatga taagggaaaa gctccttctt ttctgtcgat actgtttttc
     5641 cgtcaacagc agatacccgc tccaattctt tattcgtaaa gggggtctca ttttccggca
     5701 caaagctttt cggcggttca gtcagtgacg cgtgcttgaa tattttcaca ttgtttcgtg
     5761 ctgtatattg aaatcctcta taaaaagcga catccatggg gtcatccgtc ggatccttca
     5821 cccgcacaga aagcttagcc ctatgcgtat cggcttcgct gtctatgatc tccggctgat
     5881 ccggatgctc acgttctgtt ttaaacgtcc gctcaattac tgttttattg cccgcgaggt
     5941 ccgttgctgt tatttttaga gaatgttttc ccggcaaaag ttcagaagat gacgtatggt
     6001 agggaagtgt aatgctctca ccatctaaag acgcctccgc ttcctcaatt ttgctccatt
     6061 tatcatacat atccgcctgc aggatgaggt ttcctttgta cgtttgtccc tcggtgatat
     6121 tcggctcgat atgcggcccc gaattatcta cacgcacaac caagcttaca ttttccttgc
     6181 cgtcgcttgc ttgaatatgg tgagcgcctt cagaaagctt tgatgtatcc cacttcagca
     6241 actgtgacgt aaacagtccg tcaggaagat tgaattgaaa ttgccagctt ttattcgagc
     6301 gctgattatc tccaataaag agttccttct caggagaaac cctctgatcc ctgatcaccg
     6361 tcccatccga taacaccaat cgggcgtttt tgactaagaa atcatcacgg ttttcagccg
     6421 tatcaaatgg agacaccttt gaacctgatc gtatcgtaat acgctgcagc tgcttccctt
     6481 tacggagcag caactctggt aagggaacag aataggtgcg gtatttattt gttgtatcat
     6541 caaatacctt taacactttg ccttccatcg ttaccgcgtt tttaaagtaa agattcgttt
     6601 ttctcgtatc gaacgcaaaa tacacttctt tttccatagc gggctctgtc acttgttttt
     6661 ttccgtctat ccacagctta atgctgttag tgcgcccgtc tgttgtcgct ttgagaaact
     6721 gagttcccga tagcgtgtct ctgttttcaa tgttcatccg caaaccgtgc gatttgcttg
     6781 tttgtttaat atcaatcatt ttgaccggcg ttctcgcctc gttgattccg tcccctgctg
     6841 ccacgtaata ctcaagctga tccttcccga tcagttcagg cgaatacaca atataatggt
     6901 agagattgtc gttgcggtct ttttcagtga gaatgcgctt aaacggtttg ttttcatctg
     6961 tccgatagta gaaggccgct gttttcacaa gactgcggtc ccgtatatca gcccgcagct
     7021 caatgctttc agcagggcga acaggcttgc ggtcagtcaa atcctcaatg accggctttt
     7081 ctttatcaag cttaattttt cgtttctgct caggaacttg cgcctgtaat accgttcccg
     7141 gggacggatt tttttctccg attgagatat tgagaagttc tgcggttccg tccagcggat
     7201 acttgtaaag cacagatgac agccggcgca gcgcatgttt ccgataatgc gcgaccgaaa
     7261 tatcttttcc tgtattcgtt gatatcacaa ctgaccgcgg cctagtattg gccatcccct
     7321 ctgtctcttc taataccgcg agatttttcc cttccgttaa ttgtgtctgg taatatcgat
     7381 tgaaatcctc cgccgtcaat tctggatgcc cgacaggctt cagccaaacc acaaaagttt
     7441 cgccggatgg aatggtgatc ctttcatccg gtctccaaat cagatcggag tcaggccctt
     7501 ctttcggata gcggtaacgg atatgatagt ctttaaaatc aatcgttctg tcagtcgtat
     7561 tataaatctc aataaattca taaccgtctg cacgtcccgt gttcttcgta tcaaccgcta
     7621 gctctgtgat cagaagctcc ggaatatggg cgagatcagc ctgatatcct tcaagcttca
     7681 gtttcttcgt ctccgactcg acccgctgct tcccattgga aatcacaatg taatactcaa
     7741 gctcgctgtt ccaaatcaat cgtttcggaa tttgcgccaa aaacttgtga atcgctccgg
     7801 gtgtcgggtc catcggcacc attttgtagc ccagctcatt actttgttta taaaaaagaa
     7861 atgcagaaaa tgcttgatac tcattgtcac gcacttgagc ctcaatcatc agattgcttt
     7921 tcatttcagc tttcacatcg atttgaggag gattttcaag caagggtatg taatgaggcg
     7981 tttggaatgg ttcctgcttt ataacaggga aagctttcga ttcatgatgg ccgtcagaca
     8041 aaactgaaag aaaagacaag aacaaagaag cgataactgt gtatttcctc atcaaaacac
     8101 ctcccttttc acgtattttg cgtctgaata gggaagctta tgagttaatt tttgtgcaaa
     8161 aagaacaaaa aagcgtctgc ctcttgaaaa ttccactatt acccagtaaa ctgaggatag
     8221 ttaaccagtt aacaattaaa tcagagaggc agattgatgg acaacagtat tcatgatgaa
     8281 ttatttcaag ctatacaaca attcgcatta aaaagggata aacgcgtctg gcaaaaggta
     8341 caaataccat cccacagcat gtcctcagca catctcgaac acctcaaaaa agactggaca
     8401 ttgacccagc ttcacattgt ttcctgtata cataccagcc aaaatgtgaa caacagcttc
     8461 ttagcatccc gcttacacat ctcaaaagca gctgtttcaa aagcagttca agctttgttg
     8521 aaacacaata tcatcacagt gaccaaaaaa cccggcaaca aaaaagaaat tttttatacg
     8581 ttaactgaaa gcggcagaga actggccgca ttacatgaac agcttcatga aaaagcaaag
     8641 gagcaataca aacagctctt caatgaattt tcaattgatg atttaaaaac ggtgactgcg
     8701 tttttcaact tgtgggtcaa acatatgtaa aaaaagcctc acccgatggg agagaccttt
     8761 caatcttatt gagccctgtc aaacgcaata tgaagtgaga cgggtccgcg tgtatacaga
     8821 ccggactcag cataacgaaa accttcctct aacctgatat tccgcatctt atccagcaca
     8881 atattggcta caatctcgat ttcggttttg gcaaaagctg ctcctacaca gttatggatg
     8941 ccagatccga aagcgagatg ccgggctgcg ccgctaaaag cgctcttgat gccgagatct
     9001 tcccgatgaa tattaaacac gtcaggatgt tcaaacgctt ccgggtcccg gttagctgct
     9061 ccgatcatac aaaaaacaat cgtatccttt ttgatttcca taccgccgac caccgtatct
     9121 tgggacagct gccgcggaat cagctgaacc ggcggtttat aacgcaacgt ctccgcaatg
     9181 gctctcggca ccaacgaacg gtcagccaaa acatcattca tctgctcggg attgttgagc
     9241 aaatgatata ccatcagcgc cagcgtctta tctgccggtt ccgttgcggc taacagcaca
     9301 ttgagaatca gtgctaatat atccttgtcc gacatcgcca tgccttcata ttcagacgta
     9361 cacaggatgg aaattaaatc tgatcccgga ttgacgcggc gttcctcgat gaccggcatc
     9421 aagtaatcgg aaagttgttc gctgcaccat aaagaatgcg cccgcgcttc aggagtctga
     9481 aagatactcg taataaaatc ggcaactccg ctgtgccact cagcgatttt ttcatgatct
     9541 cttttatcca gtccgagcat gtccatcgtg acacacaccg caaacgtctt tccaaaatca
     9601 ttgacgagat cgattctccc tttttctaga taaggatcta ataagttttc tgcattttgc
     9661 ttaatgaatg gagacagatg atccaatgcg tccccgacaa agcttctcac tacaattctt
     9721 ctttttgcgg agtgttcttt cccatgcatt tgggccagca cagggccgcg catgaccggt
     9781 tcagcacgct caacaagtga ttttgtcgta aaaatatccg gatgctgaag gatatagcgg
     9841 acatcatgat agcggctgat aaaataactg tctatcgact cttcataatg aacaggatct
     9901 tcctcccgca gctgtgaaaa ataagcatat ggattttctt gaaattgatc agaaagcaca
     9961 ctaaacaact taattgattg attcatgttt actcccccta tcatccttca gatgtgatcc
    10021 gtgtttcaga aagcatctgc gccgtttcct gcacaatgag atacccttga ttaggccgca
    10081 tacgaataga aaattcatgg ttactgatct tctcaccaag cttccacgga cgatgataag
    10141 caaggagcgt ctcttccacc tcaagaatat ccggagctcc gataaaaaac ggcagctcag
    10201 caatgacata ttccacagct aaattcagca tatcctcact gacttcttcc atcatcaaac
    10261 cgatgccgcg tgcacgcccg atcatcgctt cacgagacat gtccaaacaa gcatgccgaa
    10321 aatgaggctg ctcaaaaaac gcatgttcaa cttcttgtct catcagctga taggcattgt
    10381 tttccgcgaa atcagaaaat gtgtgaatcg cctttgagtc cccgccatga gccgcaagag
    10441 ccctttctgc aaatcttcta tttcgtgata cctcttttct tacttttcgc tcagctttgc
    10501 ctctgggagt tccgagcgct tcaagaagat tagccgcctc atgtcccgca agcaaaacgg
    10561 aaacgctttt aaattcagcc gttgcccatc caatcaatct gtaaatataa tcctcagaaa
    10621 acctgctgtt aaatgggctg atccccacca aaacatgccg gcgccgttca aatatttctc
    10681 tgcagttttg tgtcagtgac tcagcagtaa aatctacagg ctttctttcc gtcaccattt
    10741 cggtcatctc attcacctct aaaaacttat cccgctttaa gtcatcaccc tcaaaaatga
    10801 gatggaataa ctggcacaac tgaacaagac gaatggcagg cagcacttaa agtttctaag
    10861 tgaattattt taagatttaa aacttctttt tcattattca tatctgatca atgtttacta
    10921 gtaaacatta gtcattttat cccttatcgt tccacccgtc aatatgaata gtacctattt
    10981 ttttgaaata aatgtaaatg tcacattttt cacacacttt ttacactcct gttcagcatg
    11041 acataacacc ggataaagtt taaacatcag caattgacaa cgaaagtaag aaaagataaa
    11101 attaaatcgt tcaccagtaa acaattttat ttattgtttc atcgtaaact attattcctc
    11161 agaaagaagg tagcctatgc ctgatttgac aaagcagatg atatacgaca tatatgtgag
    11221 actgctgcac cttaatgaac aaaaagcaaa cacttcactt cagcaatttt ttaaggaggc
    11281 cgcagaagag gatgtgatcg atatccctaa aaacatgaca agcattcatg tcattgactg
    11341 catcggccag catgagccca ttaacaatgc ggggatcgcc aaaaaaatga acttatcgaa
    11401 agcgaatgta acgaaaatca gcacaaagct gatcaaggaa gaattcatta acagctatca
    11461 gctgacagat aacaaaaaag aagtgtattt caaattaacc cgtaaaggca aacggatttt
    11521 cgatttgcat gagaaactgc ataaaaaaaa ggagctggcc ttttaccaat tcctcgattc
    11581 gttttcacaa gatgagcaaa atgctgtatt gaaattttta gagcagctga cgtctacact
    11641 tgaagcagaa caaaccgatg ggactccgga caaacctgta aagtaaacaa aaggcggtgt
    11701 attttttatg gctcaaatga aatcaaaagc ggtattgatc ttatacattg tttgcttcag
    11761 cgcatttttt gcatctttaa gccaaaacat ttattcacct ataatcccga tcattaaaga
    11821 atcgttccat gtttctgcag caatggtgaa tctgtcagtc tccgtcttta tgattgtgac
    11881 agcgattatg caaattatat tagggaccat catcgatttt aaaggcgcgc ggtttgtctt
    11941 gattgccgga attctgacaa cgacagcagc cagcatcggc tgtgcggtga ccactgactt
    12001 tactttgttt ctgatattca gaatgataca ggctgccggg tccgcagcac tgcctctcat
    12061 tgctgccaca acgatcggac agctttttac aggcagtgaa cgcgggagtg cgatgggaac
    12121 gtatcaaatg ctcctttccg ccgcgccggc tgtcgcccct gtcctcggcg gattcatagg
    12181 cggagcagcc ggatacgaag ggatcttctg gatgctcacg gccatctctg tcgttatgct
    12241 ggttgcaaac agcatgattt ttcctaagga ctctcccgca ggatctaagc agaaaaccaa
    12301 aggcaatgtg ttcactcatt acaaatcaat atttacaaat cgaacaggaa acgtcatttt
    12361 ggccttaagt tttgtcctct ttttcattta ttttgcggtg atcgtctacc tcccgatatt
    12421 actgacggag cattatcata ttgatgtgag catagcagga ttgttatatt tgccactggc
    12481 gctaagcacg atcgcaggta cgtttttgtt taaaagaata caggcaaaaa tctcgctgaa
    12541 cacattgttt atcggcagca atgcggtcgc ggcctgcagt atcatgttgt tcgccgttac
    12601 acattccgtt tctctcgttc tcatggctct tacactggca ctgtttggca tctcgatggg
    12661 ggtcattcct cccttgtact ccacaatgat caccaatgaa tttgaacaca acagagggag
    12721 tgcaatcgga attttcaact ttatcagata cacaggcatg gcggcaggtc cgatgatatc
    12781 tgcctatctg ctcaccatga tgccgtctgc catgtccttt ggcctcttag gccttgtatt
    12841 tgccgcattg agcttttgcc ttttgcctca aatgtatccg ccgcagaagc gcgttaaaca
    12901 aaaaaaacac cacatgtaaa aaaagctgcc ttctgtggga ctgaccccat aagatg
//
