LOCUS       NZ_JALAXV010000023     10094 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain T18B2 contig_0000023, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAXV010000023
VERSION     NZ_JALAXV010000023.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 10094)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10094)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 556.3003030959693x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:40:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,343
            CDSs (total)                      :: 4,261
            Genes (coding)                    :: 4,152
            CDSs (with protein)               :: 4,152
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 74 of 109
            Pseudo Genes (incomplete)         :: 58 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 35 of 109
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:22:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10094
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAXV010000023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..10094
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>10094](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <1..>10094
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            <1..3714
                     /locus_tag="MOF15_RS21530"
                     /old_locus_tag="MOF15_21485"
     CDS             <1..3714
                     /NRPS_PKS="Domain: AMP-binding (192-585). E-value:
                     9.3e-116. Score: 378.7. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21530_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (690-756). E-value: 2.8e-24. Score:
                     77.1. Matches aSDomain: nrpspksdomains_MOF15_RS21530_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (768-1070). E-value:
                     1.8e-163. Score: 537.1. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21530_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (1076-1230). E-value: 5e-53.
                     Score: 171.0. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21530_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389714.1"
                     /locus_tag="MOF15_RS21530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_21485"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268284462.1"
                     /sec_met_domain="AMP-binding (E-value: 9.3e-124, bitscore:
                     400.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.2e-16, bitscore:
                     48.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.8e-78, bitscore:
                     250.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AGRPHKDLEPILGMFVNTLALRTRPEGGKPFVEYLQEVRETALEA
                     FEHQDYPFEELVDKLKLTRDMSRNPLFDVMFVLQNMDQESIQLEELHLKPAANNGHQTS
                     KFDLTLYAHEQPCGLLTFQMEFSTDLYKKKTIEKWLQYFINMLLSITQDNKAALGTINI
                     LNEDETHYLIHELNRTKIDYPRNETISRLFEMQAEQTPNAIAIVSDTQELTYADLNSRA
                     NQIASVLQRKGVGPDRVVALLTGRTPELIAGMLGILKAGGAYLPIAPDLPVERIAYMLS
                     DSGATLLLRSEKAEKRQLDIACEQFIIEDIQKQGETKNIESSAGPHSLAYIIYTSGSTG
                     KPKGVMIEQRSVIRLVKNSNYITFTPADRLLMTSSIGFDVVTFEIFGPLLNGAALHLSD
                     KEVFLDSYQLQRYIEHHGITTMWLTSSLFNHLTEQNETTFSQLNHLIIGGEALSASHVN
                     RIRNVCPEVSIWNGYGPTENTTFSTCFHIQKTYELSIPIGRPVGNSTAYILNKWGMLQP
                     VGTVGELCVGGDGVARGYLGRPDLTKEKFVPNPFAPGDRLYRTGDLARWLPDGTIEYVG
                     RIDDQVKVRGYRVEMGEIESALRQIDGVKEAAVLARTGQTGSKELFGYISVNAGTNAEQ
                     VRSLLARSLPNYMIPAYIIEMETLPLTSNGKLNRKALPEPDFASKQTYVPPRDDLEEQL
                     ALIWQEVLGIQRIGIEDSFFELGGDSIKALQVSARLGRHGLSLQVSDLFRHPKIKDLSP
                     FICKTERIIEQGPIQGDVPWTPVQQWFLSQDIEERHHFNQSVMLFHSDRLSEDALRAAL
                     KKLAEHHDALRMVYRNDDGRWIQINQGIQESQLYSLRISDLSQSESGWETQIKQEVADL
                     QQSINLQEGPLLHAAWFKTLTGDYLFLAIHHLVVDGVAWRILLEDLSAGYQQASSGQVI
                     QLPPKTDSYQVYARRIQEYAQSSRLIREEAYWRTVEEQQAAELPYEMPLHENTDFSRRD
                     SIRFSLSAANTAVLLQNVNHAYGTDTQDILLTAASLAICEWTGGSKLRIAMEGHGREHI
                     LPELDISRTVGWFTSMYPALISFENHRDELGTAVKTVKDTLGRIPNKGVGYGILKYLTH
                     PENKSIAFSKTPDISFNYLGQFNDIERQDSFRPSSLGSGKDITHTWKREQIIEMSAMTA
                     DKKLHFNLSYPPAIFHRNTMEQLINRIEHFLRDIMKHCAGKQKAEKTLSDFSSQSLTAE
                     DLDSISSLVEEL"
     source          1..10094
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="T18B2"
                     /submitter_seqid="contig_0000023"
     region          1..10094
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10094
                     /SMILES="NC([*])C(=O)N1C(CCC1)C(=O)NC(CCC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       4..69
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0001"
                     /evalue="3.00E-09"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="22"
                     /protein_start="0"
                     /score="29.3"
                     /tool="antismash"
                     /translation="AGRPHKDLEPILGMFVNTLALR"
     CDS_motif       118..237
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0002"
                     /evalue="2.50E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="78"
                     /protein_start="38"
                     /score="74.5"
                     /tool="antismash"
                     /translation="RETALEAFEHQDYPFEELVDKLKLTRDMSRNPLFDVMFVL"
     aSDomain        580..1758
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21530_AMP-binding.1"
                     /evalue="9.30E-116"
                     /label="MOF15_RS21530_AMP-binding.1"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="585"
                     /protein_start="192"
                     /score="378.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEMQAEQTPNAIAIVSDTQELTYADLNSRANQIASVLQRKGVGPD
                     RVVALLTGRTPELIAGMLGILKAGGAYLPIAPDLPVERIAYMLSDSGATLLLRSEKAEK
                     RQLDIACEQFIIEDIQKQGETKNIESSAGPHSLAYIIYTSGSTGKPKGVMIEQRSVIRL
                     VKNSNYITFTPADRLLMTSSIGFDVVTFEIFGPLLNGAALHLSDKEVFLDSYQLQRYIE
                     HHGITTMWLTSSLFNHLTEQNETTFSQLNHLIIGGEALSASHVNRIRNVCPEVSIWNGY
                     GPTENTTFSTCFHIQKTYELSIPIGRPVGNSTAYILNKWGMLQPVGTVGELCVGGDGVA
                     RGYLGRPDLTKEKFVPNPFAPGDRLYRTGDLARWLPDGTIEYVGRIDDQVKVR"
     aSModule        580..3693
                     /complete
                     /domains="nrpspksdomains_MOF15_RS21530_AMP-binding.1"
                     /domains="nrpspksdomains_MOF15_RS21530_PCP.1"
                     /domains="nrpspksdomains_MOF15_RS21530_Epimerization.1"
                     /domains="nrpspksdomains_MOF15_RS21530_TIGR01720.1"
                     /locus_tags="MOF15_RS21530"
                     /monomer_pairings="X -> D-X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       766..804
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0003"
                     /evalue="1.70E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="267"
                     /protein_start="254"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LGILKAGGAYLPI"
     CDS_motif       991..1047
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0004"
                     /evalue="1.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="348"
                     /protein_start="329"
                     /score="33.3"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGKPKGVMIE"
     CDS_motif       1552..1641
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0005"
                     /evalue="8.20E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="546"
                     /protein_start="516"
                     /score="56.5"
                     /tool="antismash"
                     /translation="PVGTVGELCVGGDGVARGYLGRPDLTKEKF"
     CDS_motif       1723..1788
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0006"
                     /evalue="1.60E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="595"
                     /protein_start="573"
                     /score="36.1"
                     /tool="antismash"
                     /translation="YVGRIDDQVKVRGYRVEMGEIE"
     aSDomain        2074..2271
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21530_PCP.1"
                     /evalue="2.80E-24"
                     /label="MOF15_RS21530_PCP.1"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="756"
                     /protein_start="690"
                     /score="77.1"
                     /tool="antismash"
                     /translation="EEQLALIWQEVLGIQRIGIEDSFFELGGDSIKALQVSARLGRHGL
                     SLQVSDLFRHPKIKDLSPFIC"
     aSDomain        2308..3213
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21530_Epimerization.1"
                     /evalue="1.80E-163"
                     /label="MOF15_RS21530_Epimerization.1"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="1070"
                     /protein_start="768"
                     /score="537.1"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="GDVPWTPVQQWFLSQDIEERHHFNQSVMLFHSDRLSEDALRAALK
                     KLAEHHDALRMVYRNDDGRWIQINQGIQESQLYSLRISDLSQSESGWETQIKQEVADLQ
                     QSINLQEGPLLHAAWFKTLTGDYLFLAIHHLVVDGVAWRILLEDLSAGYQQASSGQVIQ
                     LPPKTDSYQVYARRIQEYAQSSRLIREEAYWRTVEEQQAAELPYEMPLHENTDFSRRDS
                     IRFSLSAANTAVLLQNVNHAYGTDTQDILLTAASLAICEWTGGSKLRIAMEGHGREHIL
                     PELDISRTVGWFTSMYPALIS"
     CDS_motif       2407..2484
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0007"
                     /evalue="1.70E-05"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="827"
                     /protein_start="801"
                     /score="17.2"
                     /tool="antismash"
                     /translation="RLSEDALRAALKKLAEHHDALRMVYR"
     CDS_motif       2689..2754
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0008"
                     /evalue="3.20E-11"
                     /label="NRPS-E2"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="917"
                     /protein_start="895"
                     /score="35.4"
                     /tool="antismash"
                     /translation="LFLAIHHLVVDGVAWRILLEDL"
     CDS_motif       3160..3207
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0009"
                     /evalue="5.90E-09"
                     /label="NRPS-E5"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="1068"
                     /protein_start="1052"
                     /score="27.9"
                     /tool="antismash"
                     /translation="DISRTVGWFTSMYPAL"
     aSDomain        3232..3693
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21530_TIGR01720.1"
                     /evalue="5.00E-53"
                     /label="MOF15_RS21530_TIGR01720.1"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="1230"
                     /protein_start="1076"
                     /score="171.0"
                     /tool="antismash"
                     /translation="ELGTAVKTVKDTLGRIPNKGVGYGILKYLTHPENKSIAFSKTPDI
                     SFNYLGQFNDIERQDSFRPSSLGSGKDITHTWKREQIIEMSAMTADKKLHFNLSYPPAI
                     FHRNTMEQLINRIEHFLRDIMKHCAGKQKAEKTLSDFSSQSLTAEDLDSI"
     CDS_motif       3256..3318
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21530_0010"
                     /evalue="7.50E-10"
                     /label="NRPS-E6"
                     /locus_tag="MOF15_RS21530"
                     /protein_end="1105"
                     /protein_start="1084"
                     /score="31.0"
                     /tool="antismash"
                     /translation="VKDTLGRIPNKGVGYGILKYL"
     aSDomain        3739..3828
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21535_NRPS-COM_Nterm.1"
                     /evalue="2.00E-18"
                     /label="MOF15_RS21535_NRPS-COM_Nterm.1"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="30"
                     /protein_start="0"
                     /score="57.3"
                     /tool="antismash"
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDS"
     gene            3739..>10094
                     /locus_tag="MOF15_RS21535"
                     /old_locus_tag="MOF15_21490"
     CDS             3739..>10094
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (0-30). E-value: 2e-18.
                     Score: 57.3. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21535_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (7-304). E-value:
                     5.4e-118. Score: 385.4. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21535_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (468-865). E-value:
                     5.9e-104. Score: 339.8. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21535_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (971-1040). E-value: 1.2e-23. Score:
                     75.0. Matches aSDomain: nrpspksdomains_MOF15_RS21535_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1054-1333). E-value:
                     3.3e-104. Score: 340.0. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21535_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1499-1897). E-value:
                     4.8e-110. Score: 359.9. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21535_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (1999-2068). E-value: 2.4e-26.
                     Score: 83.7. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21535_PCP.2"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1209: polyketide synthase associated protein papA3"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389713.2"
                     /locus_tag="MOF15_RS21535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_21490"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268284463.1"
                     /sec_met_domain="PP-binding (E-value: 4.9e-17, bitscore:
                     49.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.9e-90, bitscore:
                     289.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.6e-119, bitscore:
                     385.0, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKANSIQDIYPLSYMQEGMLFHSLLQKDSQAYVEQASFTIEGKV
                     NSQFFQNSINALVARHDIFRTIFISQNVSSPQQVVLRERNIIVQEKDITHLNEEKQAQF
                     IEQLKEKDRAQGFHLQKDMLMRIALIQTGESQYTCIWTFHHIMMDGWCLSIVLKEFLHI
                     YASYVNATPISLEPVQPYGSYIKWLMEQDKEQAVSYWEHYLSGHEQQTMLPKQKKTKGR
                     SRQEHVTFSFSKEESSRLSEIAAREEVTLSTIFHTIWGILLQKYNNNDDAVFGSVISGR
                     PAEIEGIEHMVGLFINTIPIRVQGAKTPFIQLIKDMQKDRLAAEAYSYHPLYEIQARSA
                     VKQGLIDHILVFENYPVQQEIQMLNKQEHASDLFQIHNFTVADETNYSFYLMVAPGEEI
                     HIKMSYDAEQHDRSFVLSVKEHLLNAVSQILNNPNLPPEEIEITTDTEKRQLIGEIADR
                     TPVYETIHTMFEKQAEKTPDAHAVIDQACTLTYKELNKSANRLARHLRMKGVARQEPVA
                     IMMERSAALITGVLGILKAGGAIVPIDPHYPADRIRYILNDCGCSHVVSQAHLSASLDN
                     NYLITHAEDIESTEDGSNIKSVNNADDLLYMIYTSGTTGKPKGVQFEHRNMANLLKFEF
                     AHSGIDFEADVLQFATPAFDVCYQEIFSALLKGGTLHIVPEAIKRDVPQLFAFINKHQT
                     NIVFLPTAFIKMIFSERQLANSFPYGVKHLITAGEQLIISDIFQDVLRERGIHLHNHYG
                     PSETHVVSTYTIHPGDPIPEFPPIGKPIGCTDLYILNHQKQLQPCGVPGELYISGASVA
                     RGYVNHDKLTRDKFSSDPFRPGAIMYRTGDLARRLGDGNIEYIGRADNQVKIRGYRIEP
                     QEIEVTLMNHPDIKEAAILISQDQNGEHELCAYYCSVQKLNAIDLRSYMASELPEYMIP
                     AKWIWVDSIPLTPNGKVDQAALPAPDASISGNPYAAPRNLIEAKLSQIFEDVLKNGHIG
                     IQDNFFDHGGHSLKATVLMSRIAKEFHVQVPLKDIFAHPTVEGLALIIQDAEENPYAAI
                     EPAEERKTYPVSSAQKRIYVLQQLDEGVAYNMPAVLELEGALDVAKLSAVCKELISRHE
                     PLRTSFVSGADGEPVQRIHTEVPFTLSIETAIERFIRPFDLSQAPLFRAGLIKVTNEKH
                     VLLVDMHHIISDGVSVQLLIREFTDLYANRQLKPLRIQYKDYAVWQQQFKKGDSYQKQE
                     TYWQQQFSGDLPALELPTDKRRPAERHFTGGKVTFQLDKEITARIKRLANKNRSTLYMT
                     LLALYSAFLSRLSGQDDIVIGSPIAGRPHADLEAVLGMFVNTLALRTRPAGNKTFEEFL
                     KEVRQTALEAYEHQDYPFEELVDKLGVQREMSRNPLFDTTLVLQNMEQQKLKMKDVQLR
                     WNDLQHPISKFDISLYVTEHDSELFCQFEYSTALFEKETIQRWAGLFTTLVEHTAVSPE
                     TELDDIPLLTKEEERALIELCHPFQETGYPMKQTLHYALEQQAAKTPDQPAVIFEHGAM
                     TYKELNEQANRIAWELIGRGVKSETTVAIIGKRSPEMLIGIYGILKAGGAYLPIDPDYP
                     EERISFLLEDSGTDILLLQSSSLHVPAFAGEIVYLNQANKHHLSNPNVDVLPKSLAYVI
                     YTSGSTGRPKGVEVEHRSAVNFLNSLQSRYRLKQSDVIMHKTSYSFDASIWELFWWPYA
                     GASVYLLPQGGEKEPEVIAKAVEEQEITAMHFVPSMLHAFLEHIKYRPVPIKTNRLKRV
                     FSGGEQLGTHLVSRFCELLPDVALTNSYGPTEATVEAAFFDCPLHEKLERVPIGKSVHH
                     VRMYILNQKQRMLPAGCIGELYIAGAGVARGYLNRPALTEERFLEDPFFPGERMYKTGD
                     LARWLPDGHVEFLGRLDDQVKIRGYRIEPGEIEAALRGIEGIREAAVTVRTDSGDPELC
                     AYTEGLRRNEVRTQLERLLPSYMIPAHMIEMERWPVTPSGKLDRNALPAPGGAADEEAY
                     TAPRNVTEMKLAQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFDVQVALKD
                     VFAHPTVEGLASVIREGTDSPYEAMKPAEKREAYPVSSAQKRIYVLQQLEDGGTGYNMP
                     AVLELEGKL"
     aSDomain        3760..4650
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21535_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="5.40E-118"
                     /label="MOF15_RS21535_Condensation_DCL.1"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="304"
                     /protein_start="7"
                     /score="385.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="QDIYPLSYMQEGMLFHSLLQKDSQAYVEQASFTIEGKVNSQFFQN
                     SINALVARHDIFRTIFISQNVSSPQQVVLRERNIIVQEKDITHLNEEKQAQFIEQLKEK
                     DRAQGFHLQKDMLMRIALIQTGESQYTCIWTFHHIMMDGWCLSIVLKEFLHIYASYVNA
                     TPISLEPVQPYGSYIKWLMEQDKEQAVSYWEHYLSGHEQQTMLPKQKKTKGRSRQEHVT
                     FSFSKEESSRLSEIAAREEVTLSTIFHTIWGILLQKYNNNDDAVFGSVISGRPAEIEGI
                     EHMVGLFINTIPIRVQ"
     aSModule        3760..6858
                     /complete
                     /domains="nrpspksdomains_MOF15_RS21535_Condensation_DCL.1"
                     /domains="nrpspksdomains_MOF15_RS21535_AMP-binding.1"
                     /domains="nrpspksdomains_MOF15_RS21535_PCP.1"
                     /locus_tags="MOF15_RS21535"
                     /monomer_pairings="Pro -> Pro"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       3769..3810
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0001"
                     /evalue="1.00E-04"
                     /label="C1_DCL_004-017"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="24"
                     /protein_start="10"
                     /score="14.3"
                     /tool="antismash"
                     /translation="YPLSYMQEGMLFHS"
     CDS_motif       3832..3945
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0002"
                     /evalue="1.30E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="69"
                     /protein_start="31"
                     /score="46.4"
                     /tool="antismash"
                     /translation="AYVEQASFTIEGKVNSQFFQNSINALVARHDIFRTIFI"
     CDS_motif       4165..4230
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0003"
                     /evalue="5.60E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="164"
                     /protein_start="142"
                     /score="34.8"
                     /tool="antismash"
                     /translation="FHHIMMDGWCLSIVLKEFLHIY"
     CDS_motif       4276..4314
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0004"
                     /evalue="2.40E-02"
                     /label="C4_DCL_171-183"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="192"
                     /protein_start="179"
                     /score="7.8"
                     /tool="antismash"
                     /translation="PYGSYIKWLMEQD"
     CDS_motif       4549..4644
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0005"
                     /evalue="9.40E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="302"
                     /protein_start="270"
                     /score="63.0"
                     /tool="antismash"
                     /translation="DAVFGSVISGRPAEIEGIEHMVGLFINTIPIR"
     CDS_motif       4690..4806
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0006"
                     /evalue="1.20E-20"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="356"
                     /protein_start="317"
                     /score="66.0"
                     /tool="antismash"
                     /translation="QKDRLAAEAYSYHPLYEIQARSAVKQGLIDHILVFENYP"
     aSDomain        5143..6333
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21535_AMP-binding.1"
                     /evalue="5.90E-104"
                     /label="MOF15_RS21535_AMP-binding.1"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="865"
                     /protein_start="468"
                     /score="339.8"
                     /specificity="substrate consensus: Pro"
                     /tool="antismash"
                     /translation="FEKQAEKTPDAHAVIDQACTLTYKELNKSANRLARHLRMKGVARQ
                     EPVAIMMERSAALITGVLGILKAGGAIVPIDPHYPADRIRYILNDCGCSHVVSQAHLSA
                     SLDNNYLITHAEDIESTEDGSNIKSVNNADDLLYMIYTSGTTGKPKGVQFEHRNMANLL
                     KFEFAHSGIDFEADVLQFATPAFDVCYQEIFSALLKGGTLHIVPEAIKRDVPQLFAFIN
                     KHQTNIVFLPTAFIKMIFSERQLANSFPYGVKHLITAGEQLIISDIFQDVLRERGIHLH
                     NHYGPSETHVVSTYTIHPGDPIPEFPPIGKPIGCTDLYILNHQKQLQPCGVPGELYISG
                     ASVARGYVNHDKLTRDKFSSDPFRPGAIMYRTGDLARRLGDGNIEYIGRADNQVKIR"
     CDS_motif       5329..5370
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0007"
                     /evalue="3.10E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="544"
                     /protein_start="530"
                     /score="14.1"
                     /tool="antismash"
                     /translation="LGILKAGGAIVPID"
     CDS_motif       5554..5610
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0008"
                     /evalue="1.50E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="624"
                     /protein_start="605"
                     /score="26.8"
                     /tool="antismash"
                     /translation="YMIYTSGTTGKPKGVQFEH"
     CDS_motif       5992..6018
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0009"
                     /evalue="4.80E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="760"
                     /protein_start="751"
                     /score="4.0"
                     /tool="antismash"
                     /translation="YGPSETHVV"
     CDS_motif       6127..6216
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0010"
                     /evalue="5.80E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="826"
                     /protein_start="796"
                     /score="41.0"
                     /tool="antismash"
                     /translation="PCGVPGELYISGASVARGYVNHDKLTRDKF"
     CDS_motif       6298..6363
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0011"
                     /evalue="7.00E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="875"
                     /protein_start="853"
                     /score="37.2"
                     /tool="antismash"
                     /translation="YIGRADNQVKIRGYRIEPQEIE"
     aSDomain        6652..6858
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21535_PCP.1"
                     /evalue="1.20E-23"
                     /label="MOF15_RS21535_PCP.1"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1040"
                     /protein_start="971"
                     /score="75.0"
                     /tool="antismash"
                     /translation="EAKLSQIFEDVLKNGHIGIQDNFFDHGGHSLKATVLMSRIAKEFH
                     VQVPLKDIFAHPTVEGLALIIQDA"
     aSDomain        6901..7737
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21535_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.30E-104"
                     /label="MOF15_RS21535_Condensation_LCL.1"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1333"
                     /protein_start="1054"
                     /score="340.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="KTYPVSSAQKRIYVLQQLDEGVAYNMPAVLELEGALDVAKLSAVC
                     KELISRHEPLRTSFVSGADGEPVQRIHTEVPFTLSIETAIERFIRPFDLSQAPLFRAGL
                     IKVTNEKHVLLVDMHHIISDGVSVQLLIREFTDLYANRQLKPLRIQYKDYAVWQQQFKK
                     GDSYQKQETYWQQQFSGDLPALELPTDKRRPAERHFTGGKVTFQLDKEITARIKRLANK
                     NRSTLYMTLLALYSAFLSRLSGQDDIVIGSPIAGRPHADLEAVLGMFVNTLALRTRP"
     aSModule        6901..9942
                     /complete
                     /domains="nrpspksdomains_MOF15_RS21535_Condensation_LCL.1"
                     /domains="nrpspksdomains_MOF15_RS21535_AMP-binding.2"
                     /domains="nrpspksdomains_MOF15_RS21535_PCP.2"
                     /locus_tags="MOF15_RS21535"
                     /monomer_pairings="Glu -> Glu"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       6967..7080
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0012"
                     /evalue="7.50E-15"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1114"
                     /protein_start="1076"
                     /score="47.3"
                     /tool="antismash"
                     /translation="AYNMPAVLELEGALDVAKLSAVCKELISRHEPLRTSFV"
     CDS_motif       7252..7317
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0013"
                     /evalue="1.90E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1193"
                     /protein_start="1171"
                     /score="30.0"
                     /tool="antismash"
                     /translation="MHHIISDGVSVQLLIREFTDLY"
     CDS_motif       7348..7380
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0014"
                     /evalue="1.10E-03"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1214"
                     /protein_start="1203"
                     /score="11.8"
                     /tool="antismash"
                     /translation="QYKDYAVWQQQ"
     CDS_motif       7639..7728
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0015"
                     /evalue="2.80E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1330"
                     /protein_start="1300"
                     /score="51.8"
                     /tool="antismash"
                     /translation="DIVIGSPIAGRPHADLEAVLGMFVNTLALR"
     CDS_motif       7777..7896
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0016"
                     /evalue="7.00E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1386"
                     /protein_start="1346"
                     /score="66.7"
                     /tool="antismash"
                     /translation="RQTALEAYEHQDYPFEELVDKLGVQREMSRNPLFDTTLVL"
     aSDomain        8236..9429
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21535_AMP-binding.2"
                     /evalue="4.80E-110"
                     /label="MOF15_RS21535_AMP-binding.2"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1897"
                     /protein_start="1499"
                     /score="359.9"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="LEQQAAKTPDQPAVIFEHGAMTYKELNEQANRIAWELIGRGVKSE
                     TTVAIIGKRSPEMLIGIYGILKAGGAYLPIDPDYPEERISFLLEDSGTDILLLQSSSLH
                     VPAFAGEIVYLNQANKHHLSNPNVDVLPKSLAYVIYTSGSTGRPKGVEVEHRSAVNFLN
                     SLQSRYRLKQSDVIMHKTSYSFDASIWELFWWPYAGASVYLLPQGGEKEPEVIAKAVEE
                     QEITAMHFVPSMLHAFLEHIKYRPVPIKTNRLKRVFSGGEQLGTHLVSRFCELLPDVAL
                     TNSYGPTEATVEAAFFDCPLHEKLERVPIGKSVHHVRMYILNQKQRMLPAGCIGELYIA
                     GAGVARGYLNRPALTEERFLEDPFFPGERMYKTGDLARWLPDGHVEFLGRLDDQVKIR"
     CDS_motif       8425..8463
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0017"
                     /evalue="9.10E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1575"
                     /protein_start="1562"
                     /score="15.8"
                     /tool="antismash"
                     /translation="GILKAGGAYLPID"
     CDS_motif       8641..8700
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0018"
                     /evalue="3.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1654"
                     /protein_start="1634"
                     /score="31.9"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVEVEH"
     CDS_motif       9085..9114
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0019"
                     /evalue="4.60E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1792"
                     /protein_start="1782"
                     /score="6.9"
                     /tool="antismash"
                     /translation="SYGPTEATVE"
     CDS_motif       9223..9312
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0020"
                     /evalue="6.00E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1858"
                     /protein_start="1828"
                     /score="56.9"
                     /tool="antismash"
                     /translation="PAGCIGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       9394..9459
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21535_0021"
                     /evalue="1.20E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="1907"
                     /protein_start="1885"
                     /score="39.6"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
     aSDomain        9736..9942
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21535_PCP.2"
                     /evalue="2.40E-26"
                     /label="MOF15_RS21535_PCP.2"
                     /locus_tag="MOF15_RS21535"
                     /protein_end="2068"
                     /protein_start="1999"
                     /score="83.7"
                     /tool="antismash"
                     /translation="EMKLAQLWEDVLKNGPVGIHDNFFDRGGHSLKATALVSRIAKEFD
                     VQVALKDVFAHPTVEGLASVIREG"
ORIGIN
        1 gccggccggc cgcacaagga tcttgagccg atactcggca tgtttgtgaa cacgctggcg
       61 cttcggacac gcccggaggg cgggaagccg tttgtggagt atttgcagga agtgcgcgag
      121 acagcattgg aagcctttga acatcaggat tatccgttcg aagagctcgt cgacaagctt
      181 aaattgacgc gggatatgag ccggaatccg ctgtttgacg tgatgtttgt tcttcaaaat
      241 atggatcagg aatcgattca gcttgaagaa ctgcatctca aacctgcagc aaacaacggg
      301 catcaaacgt ccaaatttga tttaactctg tatgctcacg aacagccctg cggtttgctg
      361 acgttccaaa tggaatttag tacggactta tataagaaaa aaacgataga aaagtggctt
      421 caatacttca tcaatatgtt actctccatc acccaagaca acaaagccgc tctcggaaca
      481 ataaatatac taaatgagga tgagacccat taccttatcc atgagctcaa tcgtacaaaa
      541 atagattatc caagaaatga aaccatcagc aggctctttg aaatgcaggc tgaacaaaca
      601 cctaatgcca ttgcaattgt cagcgataca caagaattaa cttacgcgga tctgaacagc
      661 cgggcaaatc aaattgcttc agttctacag agaaagggag tagggcctga tcgtgttgta
      721 gccttgttga caggacgaac gccagaactc atagcgggaa tgctgggtat tctgaaagcc
      781 ggaggagcct acctgccaat cgcacctgat ctgcctgttg aacgaatagc ttatatgctg
      841 tcagacagcg gggcaacatt actccttcga tctgaaaaag cagaaaaacg gcaattagac
      901 attgcatgtg aacaattcat aatcgaagac attcaaaagc aaggagaaac aaagaacatt
      961 gaatcgtcag cagggccaca ttcacttgca tatatcattt atacctctgg atcaaccggt
     1021 aaaccaaagg gtgtcatgat tgagcagcga agtgtaattc gtcttgttaa aaacagcaat
     1081 tatattacat ttacacctgc agaccgtctt ctcatgacat catcaatcgg ttttgatgtt
     1141 gtgacatttg aaatatttgg accgctgcta aatggggcag ctttgcacct gtccgataaa
     1201 gaagtatttc tcgattccta tcagcttcag agatacatag aacatcatgg gatcacaacg
     1261 atgtggttga catcttctct attcaatcat ttgacagagc aaaatgagac aaccttttca
     1321 cagctgaatc atcttattat aggcggggaa gccttgtccg caagccacgt caatcgaatc
     1381 agaaatgtct gccctgaagt atcgatatgg aacggatatg gaccaaccga aaatacaact
     1441 ttttcgacat gcttccacat tcaaaaaaca tatgaacttt ccattccgat cgggcggccg
     1501 gttggtaatt ccactgctta tatcttaaat aaatggggaa tgctgcagcc ggtcggaact
     1561 gtcggtgaac tttgtgtcgg cggtgatggc gtcgccagag gctatttagg ccgtcctgat
     1621 ttaacaaaag agaaatttgt cccaaatcca tttgcgcctg gcgatcgact gtaccgcaca
     1681 ggtgatctgg cgcgttggct tcccgatggc acaattgagt acgttggccg tatagatgat
     1741 caagtaaaag tcagaggcta tcgggtggag atgggtgaga tcgaatcagc acttcgccaa
     1801 atagacgggg tcaaagaagc cgctgtgttg gcacgtacag gccagacagg gagcaaagag
     1861 ctgtttggct acatcagcgt aaatgcaggc acgaatgctg aacaagtgcg ttctcttctc
     1921 gcgcgttcac tgccaaatta tatgatcccg gcgtacatca ttgaaatgga gactcttccg
     1981 ctcacatcaa acggcaaatt gaaccggaaa gcacttcctg aaccagattt cgcatctaaa
     2041 caaacgtacg tcccgccacg cgatgacctt gaagaacagc tggccttgat ctggcaggag
     2101 gttctcggca tccagaggat cggaatagag gattcgtttt ttgaattggg cggagattca
     2161 atcaaagcgc ttcaagtttc tgcacgcctt gggcgccatg ggctaagctt gcaggtaagt
     2221 gatttattcc ggcatccaaa gataaaagac ttatcaccat ttatctgtaa gacagagaga
     2281 atcatagaac aaggccccat tcagggagat gttccttgga cgcctgttca gcaatggttt
     2341 ctttcacaag atattgaaga acgacaccat tttaatcagt ctgtcatgct tttccattct
     2401 gaccgtttgt ctgaagacgc ccttagagca gccttaaaaa aactggcgga gcatcatgat
     2461 gcgcttcgga tggtttatcg taacgatgac gggcgatgga ttcaaatcaa ccaaggcatt
     2521 caggaatcac agctgtacag tttaagaata tctgatcttt cacagtccga aagcggttgg
     2581 gaaacgcaaa tcaaacaaga agttgcagat cttcagcaaa gcatcaattt gcaggaagga
     2641 ccattgttac atgccgcgtg gtttaaaaca ctgacaggag attacttgtt ccttgcgatc
     2701 catcatcttg tcgttgacgg tgtggcatgg cggattttgc tggaggattt gtcagcaggg
     2761 taccaacaag catcttcagg ccaagtgatc cagcttccgc ctaaaactga ttcctatcag
     2821 gtatacgcaa gacggattca ggaatatgcc caaagcagca ggctgattcg tgaggaggcg
     2881 tattggcgaa ctgtggaaga acagcaagct gcggaactgc catatgaaat gccgcttcat
     2941 gagaacaccg acttcagcag aagggattcg atacgctttt cactctctgc agcaaacact
     3001 gccgttttgc tgcaaaacgt caatcacgcc tatggtacag atacacaaga tattctgtta
     3061 accgcagcat ccttggcgat ttgtgaatgg acaggcggaa gcaagctccg tatcgctatg
     3121 gaaggacacg ggagagaaca cattctgcca gagttagata tcagccggac ggtcggctgg
     3181 tttacatcca tgtacccggc actcataagc tttgaaaatc atagggatga gctcggaacc
     3241 gcagtaaaaa ccgttaaaga tacactgggc cggataccaa acaaaggggt aggttacggt
     3301 attctgaaat accttacaca ccctgaaaac aaaagcattg cgttttcaaa aacacctgac
     3361 atcagcttta actatttggg ccagttcaac gacatcgaaa gacaggacag cttccgccca
     3421 tcaagtcttg ggagcggaaa ggatatcact catacatgga agcgtgagca aatcatcgag
     3481 atgagtgcaa tgacagcaga caaaaagctc catttcaatc ttagttatcc gccagccatc
     3541 tttcatagaa acacaatgga gcagcttatc aacaggattg aacacttttt gcgggacatt
     3601 atgaaacatt gcgccggtaa acagaaagca gaaaagacac tgagcgattt tagcagtcaa
     3661 tcattaacgg cagaggattt ggacagcata tccagcttgg tggaagaatt gtagaaaatc
     3721 ggacagggag acatgaacat gacaaaagcg aattcaattc aggatattta tccgctgtct
     3781 tacatgcagg aagggatgct tttccattcc ctcctgcaaa aagattcaca ggcatatgtt
     3841 gagcaagctt cttttacaat agaaggaaaa gtcaactcgc agttttttca aaacagcatc
     3901 aacgctcttg tagcaagaca cgatattttc agaacgattt ttatcagcca aaatgtttcc
     3961 tctccccagc aggttgtcct gagagaacgg aacatcatcg tacaggaaaa agacattact
     4021 catttaaacg aagagaagca agcacagttt atcgaacaat tgaaagaaaa agaccgcgcc
     4081 caagggtttc acttgcaaaa ggatatgctc atgcgcatcg ctctgattca aacaggagag
     4141 agccagtaca cttgtatttg gacctttcat cacatcatga tggacggatg gtgcctcagc
     4201 attgtgctaa aggagtttct ccatatttac gcatcttatg taaatgccac ccctatatca
     4261 ttggagccgg tccagccata cggaagctat atcaaatggc tgatggagca agacaaagag
     4321 caggcagttt catattggga acattatctt tccggccatg aacagcaaac catgctcccg
     4381 aagcagaaaa aaacaaaggg gagaagcagg caggaacacg ttacgttttc attctctaag
     4441 gaagaaagca gcaggctttc ggagattgcg gcaagagaag aagtaacact cagtacgatt
     4501 ttccatacca tttggggtat tctgcttcaa aaatataata ataacgatga cgccgtattc
     4561 ggttctgtca tttcaggaag acccgcagaa atcgaaggca ttgaacatat ggtaggtctt
     4621 ttcattaaca cgataccgat tagggtgcaa ggggcaaaaa caccatttat acagctcata
     4681 aaagatatgc aaaaagaccg tcttgccgct gaagcataca gctaccaccc tttatatgag
     4741 attcaagccc gttcagctgt taaacaaggg ctgattgatc atattctcgt atttgaaaac
     4801 tatcctgttc agcaagagat tcagatgctg aacaagcagg aacatgcttc tgatcttttt
     4861 cagattcata acttcactgt ggcagatgaa accaactaca gcttttatct tatggtggcg
     4921 ccaggagaag aaatccatat taaaatgagc tatgatgcgg agcagcatga tcggtctttt
     4981 gtgctttctg tcaaggagca tcttctgaat gctgtatcac aaatcttaaa taatccgaat
     5041 cttccgcctg aagaaatcga gatcacgaca gatactgaaa agcggcagct gattggggaa
     5101 atcgcggatc gaacgccagt ttacgaaacc atccatacga tgtttgaaaa acaagcggaa
     5161 aagaccccag atgctcatgc agtaattgat caagcctgca cgttaacata caaagaactg
     5221 aataaatcgg ctaacagatt ggcgcggcat ttacgaatga agggtgttgc gaggcaggag
     5281 cctgttgcga tcatgatgga acgctctgcg gcattgatca ccggggttct tggtatcttg
     5341 aaagcaggcg gcgccatagt tccaattgat ccgcattacc cggctgacag aatccgttat
     5401 attctaaatg actgcggctg ttcgcatgtt gtttcacaag ctcatctttc cgcatcgctt
     5461 gataacaatt accttatcac tcatgcagaa gatattgaaa gcacagaaga cggcagtaac
     5521 ataaagtcag ttaacaatgc ggacgatctg ctgtatatga tttatacatc aggtacaaca
     5581 ggtaaaccaa agggcgttca atttgaacat cgaaacatgg ctaacttgct gaagttcgaa
     5641 tttgctcact ctgggattga ctttgaagca gatgttctgc aatttgcgac acctgccttt
     5701 gacgtctgtt atcaagaaat attttctgcg cttctgaaag gcggcaccct gcacatcgtg
     5761 ccagaagcca taaaaagaga tgtgcctcag ctgtttgcat ttataaacaa gcatcagacg
     5821 aatattgtgt ttctcccaac ggcttttatc aaaatgattt tcagcgagcg gcaacttgcg
     5881 aactcgtttc cttatggcgt caaacacttg attacagctg gggaacaatt aattatttca
     5941 gacattttcc aagacgtgtt acgtgaacgc ggcatacacc tgcacaatca ttacggacct
     6001 tcagaaactc atgtggtatc tacgtacacc attcatccgg gagaccctat tcctgagttt
     6061 ccgccaatcg gtaaaccgat aggctgtact gatctatata ttttgaatca ccaaaaacag
     6121 ctacagccat gcggtgtacc aggtgaactc tatatttccg gcgcaagtgt tgccagagga
     6181 tatgtcaatc acgataaact gacacgcgac aaattttcat ctgacccatt caggcctggt
     6241 gcgatcatgt accgtacagg tgatttggcc agacggcttg gagacggcaa catcgaatat
     6301 ataggacggg cagacaatca agtgaaaatc agaggttatc gaatcgagcc gcaagaaatc
     6361 gaagtcacgt taatgaatca tccggacatt aaagaggctg ccattctcat cagtcaagat
     6421 caaaacggag aacatgaatt atgcgcttac tattgcagtg tacaaaagct gaatgcaata
     6481 gatttacgca gctatatggc tagtgagcta ccagaataca tgattccggc gaaatggata
     6541 tgggttgaca gcatacctct taccccaaac ggaaaagtag accaagcagc gcttccagcg
     6601 ccagatgctt caatcagcgg aaatccatat gctgcccctc gtaatctgat agaggcaaag
     6661 ctttcacaaa tatttgaaga tgtgctgaag aatggtcata taggcatcca agacaacttt
     6721 tttgaccatg gaggccactc tctaaaagca acagtgctga tgtcacggat cgcaaaggaa
     6781 tttcatgtac aagttccgct taaggacatc tttgcccatc cgactgtaga aggattggct
     6841 ttaatcattc aagatgcaga ggaaaatccg tatgcagcaa ttgagccggc agaagaaagg
     6901 aagacatatc ctgtatcttc ggcacaaaaa cggatatatg tgcttcagca gcttgatgag
     6961 ggtgtcgcct acaatatgcc tgcggtctta gaactggaag gggcactgga tgtcgctaaa
     7021 ctgtctgctg tatgtaaaga actgatcagc cggcatgagc ctcttcgaac ttcctttgta
     7081 tccggggcag atggcgagcc tgttcagcgt atccacaccg aagtgccgtt tacattaagt
     7141 atagagaccg ctatcgagag gtttatccgc ccctttgatc tcagccaggc gccattattt
     7201 agagcgggcc ttataaaagt gacgaacgag aagcatgtat tgctggtgga tatgcatcat
     7261 atcatttcag atggcgtatc cgttcagctc ctaatccgag aattcaccga tctttatgca
     7321 aaccgccagc ttaagccatt gcggatccag tataaagatt acgccgtttg gcagcagcaa
     7381 tttaaaaagg gagactcgta ccaaaagcag gagacttact ggcagcagca gtttagcggg
     7441 gacctccctg cgctagagct tccgacagat aaacgccgcc cggcagaacg gcactttaca
     7501 ggcggaaaag taacctttca attggataaa gagatcactg cccgcataaa gcggcttgcc
     7561 aataaaaaca gaagcacgct ttatatgacg cttcttgctt tgtattcagc ctttttgtcg
     7621 cggttaagcg gtcaggatga tatcgtaatt ggttcaccta tcgccggacg gccccatgcc
     7681 gaccttgaag cagttcttgg gatgttcgtc aatacactgg ctctccggac acgcccggca
     7741 ggtaacaaga catttgaaga atttctgaag gaagttaggc agacggcact agaagcgtat
     7801 gagcatcagg attatccgtt tgaagagctt gtggataaac tcggcgtaca gcgggaaatg
     7861 agccggaatc cactgtttga cacaacactt gtccttcaga atatggaaca gcagaagctg
     7921 aaaatgaaag atgtacagct tcgatggaac gatttgcagc atccaatatc gaaatttgat
     7981 atttctctgt atgttacaga acacgattcc gagcttttct gtcagtttga atacagtaca
     8041 gctttgtttg agaaagaaac catccaaagg tgggccggcc ttttcaccac tcttgtggaa
     8101 catacagctg tcagccctga aacagaattg gacgatattc cactcttaac aaaagaagaa
     8161 gagagagctc tcatcgaatt atgccatccg tttcaggaga cgggctatcc gatgaagcaa
     8221 actctccact atgcacttga acagcaggca gcaaaaacgc ctgatcagcc ggccgttatc
     8281 tttgaacatg gagcgatgac ctataaagag ttgaacgaac aggctaaccg tatcgcctgg
     8341 gagctgatcg gacgaggagt aaaatctgaa acgactgttg ccattatagg aaagcgttcg
     8401 cctgaaatgc tgattggcat ttatggcatt cttaaagctg gcggcgctta tcttcctatt
     8461 gacccggatt acccggaaga acgaatcagc tttctgctgg aggacagcgg tacagacatt
     8521 ctcctgcttc agtcttccag cctgcatgtt ccggcatttg caggagagat tgtctacctc
     8581 aatcaggcaa ataaacacca tttgtccaat ccgaatgtgg acgttctccc taaatcttta
     8641 gcctatgtca tctatacatc cggctctacc ggcaggccta agggtgtgga agttgaacat
     8701 cgatctgcgg tgaatttttt gaattccctt cagtcccgct atcggctgaa gcaatcagat
     8761 gtgattatgc ataaaacatc ttactcattt gacgcttcaa tatgggagct gttttggtgg
     8821 ccttatgccg gtgcttctgt ttatctgctt cctcaaggag gggaaaaaga acctgaagtg
     8881 atcgcgaagg ccgtagaaga acaagagatc acagctatgc attttgtgcc atctatgctc
     8941 catgcatttt tagaacatat aaaatatcga cctgttccga ttaagacaaa cagactcaag
     9001 cgggtgtttt ccgggggaga acagctgggg acccatctcg tctcccgatt ttgcgagtta
     9061 cttccagatg ttgcattaac caattcttac ggaccaacgg aggcaactgt agaagctgcg
     9121 ttctttgatt gtccgctgca tgaaaagctt gaacgcgttc caattggaaa atctgttcat
     9181 catgtcagaa tgtatatcct aaatcagaag cagcggatgc tgcctgctgg atgtatcgga
     9241 gaactgtaca ttgccggagc gggtgttgcc agaggctatt taaatcggcc ggcattaacg
     9301 gaggagcgtt ttcttgagga cccgtttttc ccgggtgaac gcatgtataa aactggagat
     9361 ttggcgcgct ggcttcctga cggacatgtt gagttcctcg gccggttaga tgatcaggtg
     9421 aaaatccgcg gctaccggat tgagcccgga gaaattgaag cagcgctaag aggcatagaa
     9481 ggcatcaggg aggcagccgt taccgtacgg acagacagcg gcgacccgga attatgcgcc
     9541 tatacagaag gactaagaag aaacgaagtg cggacgcagc ttgaaagact gctgccgagc
     9601 tacatgatcc cagcccatat gatagagatg gaacggtggc cggttacgcc aagcggaaag
     9661 ctggaccgaa acgccctgcc agctcctggc ggagcggcag atgaggaggc ctacacggcg
     9721 ccgagaaatg tgacagagat gaagcttgcc cagctgtggg aggacgtgct gaaaaacggc
     9781 cctgtcggga ttcacgacaa ctttttcgac cgcggaggac attccttaaa agcaacggcg
     9841 cttgtttcac ggatcgccaa agaattcgat gtacaggtgg cgctgaaaga tgtgtttgcc
     9901 catccgactg ttgaaggact ggcttccgtt atccgtgaag gaacggacag tccatatgaa
     9961 gcgatgaagc cggctgaaaa gcgcgaggcc tatccggttt cctccgccca aaaaaggatc
    10021 tatgtccttc agcagctgga ggacggaggg acaggctaca atatgccggc tgtgttagag
    10081 ctggaaggga agct
//
