LOCUS       NZ_JALAXV010000021      7852 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain T18B2 contig_0000021, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAXV010000021
VERSION     NZ_JALAXV010000021.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 556.3003030959693x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:40:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,343
            CDSs (total)                      :: 4,261
            Genes (coding)                    :: 4,152
            CDSs (with protein)               :: 4,152
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 74 of 109
            Pseudo Genes (incomplete)         :: 58 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 35 of 109
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:22:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7852
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAXV010000021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7852
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="T18B2"
                     /submitter_seqid="contig_0000021"
     region          1..7852
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..7852
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7852
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[5259:>7852](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5260..>7852
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(43..588)
                     /gene="iseA"
                     /locus_tag="MOF15_RS21500"
                     /old_locus_tag="MOF15_21455"
     CDS             complement(43..588)
                     /codon_start=1
                     /gene="iseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389720.2"
                     /locus_tag="MOF15_RS21500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_21455"
                     /product="IseA DL-endopeptidase inhibitor family protein"
                     /protein_id="WP_225516922.1"
                     /transl_table=11
                     /translation="MKKCFLFLTTIALILSLSTNAFAKNTSGDLSQKQALQLALSAREH
                     FWNTMSGHNPKAKKAVCPSGTFEYQNLQYVYMCSDLGTKAKAVNYLTPIFTKTAIEKGF
                     KDYHFTVSKGKLAVPIGDGDNLLNWKKSTAKLISKKGGTVTYEFTVPTLDGSPAAKRKV
                     TFVKESKKWKVNQFDAVI"
     gene            875..2266
                     /locus_tag="MOF15_RS21505"
                     /old_locus_tag="MOF15_21460"
     CDS             875..2266
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389719.2"
                     /locus_tag="MOF15_RS21505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_21460"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_003241939.1"
                     /transl_table=11
                     /translation="METSQEQLVTQKKSNPSVWRSMSLFLVPLLLSNVLQSVGQLVGMM
                     AVGRWLGVDAVAAVSSFFPLFFLLISFTIGIGSGSSILIGQAYGAKNEERLKAVVGTTL
                     TFTFLLGVVLAVIGSIFTLDILRLMGTPENVIHVSASYARILFYAMPFMFLYFAYTTFL
                     RGTGDSKTPFYTLIVSTVINIALLPVLILGMFGFPKLGIYGSAYATVISTIATFIVLMV
                     YLRKRNHPLQFDKTVRRYLKMDKELLALLLRLGIPSSINMILVSLSEIAVISFVNHYGS
                     NATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANEFGRLKQVIRVGIWLNYIIGG
                     VLIILIYVFSHQILGLFLTEQETLYIAHRLLMITLWSYLLFGNAQIISATMRASGTVLW
                     PTVISIFAIWGVEVPVAFVLSHYTNLEILGVWVGYPAAFAVSLLLIYGYYQFVWKKKQI
                     TRLIQ"
     gene            2399..3376
                     /gene="galM"
                     /locus_tag="MOF15_RS21510"
                     /old_locus_tag="MOF15_21465"
     CDS             2399..3376
                     /EC_number="5.1.3.3"
                     /codon_start=1
                     /gene="galM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389718.1"
                     /locus_tag="MOF15_RS21510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_21465"
                     /product="aldose 1-epimerase"
                     /protein_id="WP_268284458.1"
                     /transl_table=11
                     /translation="MANDIEKITYLGTPAIKAGNEHIEMIVVPEWGSNVISLVDKTTQL
                     QLLREPETAESFHDTPTLYGIPILFPPNRISDGTFSFRGRTYHFDINEKDKHNHLHGFL
                     YQEKWNVVTMKQTDEEVIVETEIDLSELPHVYKQFPHHAVVRMTYTLKGNTLFKHATVM
                     NNGKEAFPWGIGYHTTFVFPEESSLFSLTADQQWELDERLLPTGKLMDVPNKEALHEGM
                     DLRSQQLDDVFLSSYQKRGGENQTVIYHQHAHISIIYKADEQFKHWVVYNADGRQGYLC
                     PEPYTWVTNAVNLDVSPSLTGVQVLEPGEETTAKSSITIELNHQ"
     gene            3411..4889
                     /gene="dacB"
                     /locus_tag="MOF15_RS21515"
                     /old_locus_tag="MOF15_21470"
     CDS             3411..4889
                     /EC_number="3.4.16.4"
                     /GO_function="GO:0004175 - endopeptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241941.1"
                     /locus_tag="MOF15_RS21515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_21470"
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="WP_268284459.1"
                     /transl_table=11
                     /translation="MKKSIKLYVAVLLLFVVASVPYMHQAAQAAEKQGALAGQIDKILT
                     DHPALEGAMASITVRSAETGAVLYEHSGDIRMRPASSLKLLTAAASLSILGEDYSFTTE
                     VRTDGKFKGKKLNGNLYLIGKGDPTLLPSDFEEMAEKLKQSGVKEIKGNLIGDDTWYDD
                     IRLSPDMPWSDEYTYYGAQISALTASPNEDYDAGTVIVEITPGKKEGEKPAVSVSPKTD
                     YVTIKNDAETTAAGQKKDLTIKREHGTNTITIEGSVPVDASKTKEWIAVWEPAGYALDL
                     FKQALKKQGITVKGHVKTGTAPGSSDVLISHHSMPLSKLFVPFMKLSNNGHAEVLVKEM
                     GKVKKEEGSWEKGLEVLNNALPEFGVDPKSLVLRDGSGVSHINAVSSDQISQLLYDIQD
                     EKWFSAYLNSLPVAGNSDRMIGGTLRNRMKDTPAQGKVRAKTGSLSTVSSLSGYAETKS
                     GKTLVFSILLNGLIDDEDGKDIEDQIAVILANQS"
     gene            5260..>7852
                     /locus_tag="MOF15_RS21520"
                     /old_locus_tag="MOF15_21475"
     CDS             5260..>7852
                     /NRPS_PKS="Domain: Condensation_Starter (3-300). E-value:
                     1.2e-88. Score: 289.3. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21520_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (463-864). E-value:
                     6.3e-113. Score: 369.4. Matches aSDomain:
                     nrpspksdomains_MOF15_RS21520_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOF15_RS21520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_21475"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_268284460.1"
                     /sec_met_domain="Condensation (E-value: 4.3e-75, bitscore:
                     240.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.2e-112, bitscore:
                     363.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQHTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVL
                     EGALNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQA
                     RIPFKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDP
                     LPDQPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSAS
                     RDTITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLSIGTYYGNRGSKA
                     EKEMLGMFVSSLPIRITVDPDADFLSFVRRVGREQLSVMRHQRFPYNLLVNELRKEQKD
                     LHNLIGISMQYQPLQWHNADGFDYETALYFSGYTANELSVQIQERTDTGTIQLNFDYQN
                     TLFSLEDIKRIQNHLLTLLENALQHPHSFIKELDMTNIREKQKLLYEFNKTEAVSPEAP
                     TLHGLFERQAAFTPERPAIRFSSGSLTYAELDMYAGRLASRLAARGVTKESIVGVLSER
                     SPEMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDSGAALLLAQSGCSAPSFSGEMLE
                     VDMASLASEKAENDVFTPADGCSLAYVIYTSGSTGKPKGVAVEHRQAVSFLTGMQRQFP
                     LQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAIHQEKVTTAH
                     FIPAMLNSFLDQAEIERLSDGTSLKRVFAGGEPLAPHTAARFASLLPQVSLIHGYGPTE
                     ATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDAHLAVQPCGVAGELYIAGAGVAR
                     GYLNRPALTEERFLEDPFYPGERMYKTGDLARWLPDGNIEFLGRTDDQVKIR"
     aSDomain        5269..6159
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21520_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="1.20E-88"
                     /label="MOF15_RS21520_Condensation_Starter.1"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="300"
                     /protein_start="3"
                     /score="289.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="HTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVLEGA
                     LNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQARIP
                     FKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDPLPD
                     QPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSASRDT
                     ITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLSIGTYYGNRGSKAEKE
                     MLGMFVSSLPIRITVD"
     aSModule        5269..7851
                     /domains="nrpspksdomains_MOF15_RS21520_Condensation_Starter
                     .1"
                     /domains="nrpspksdomains_MOF15_RS21520_AMP-binding.1"
                     /incomplete
                     /locus_tags="MOF15_RS21520"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       5275..5313
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0001"
                     /evalue="5.10E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="18"
                     /protein_start="5"
                     /score="9.8"
                     /tool="antismash"
                     /translation="YSLTHAQRRVWFT"
     CDS_motif       5338..5454
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0002"
                     /evalue="6.00E-10"
                     /label="C2_starter_024-063"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="65"
                     /protein_start="26"
                     /score="31.7"
                     /tool="antismash"
                     /translation="ICNLTACVKFRGNIDLDVLEGALNHSISRNDAIRFQLLE"
     CDS_motif       5659..5724
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0003"
                     /evalue="8.80E-08"
                     /label="C3_starter_134-155"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="155"
                     /protein_start="133"
                     /score="25.0"
                     /tool="antismash"
                     /translation="FHHIIMDGISLNVMGNQIIDLY"
     CDS_motif       6058..6147
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0004"
                     /evalue="2.70E-08"
                     /label="C5_starter_265-294"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="296"
                     /protein_start="266"
                     /score="26.2"
                     /tool="antismash"
                     /translation="DLSIGTYYGNRGSKAEKEMLGMFVSSLPIR"
     CDS_motif       6199..6312
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0005"
                     /evalue="1.70E-09"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="351"
                     /protein_start="313"
                     /score="30.2"
                     /tool="antismash"
                     /translation="REQLSVMRHQRFPYNLLVNELRKEQKDLHNLIGISMQY"
     aSDomain        6649..7851
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOF15_RS21520_AMP-binding.1"
                     /evalue="6.30E-113"
                     /label="MOF15_RS21520_AMP-binding.1"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="864"
                     /protein_start="463"
                     /score="369.4"
                     /specificity="substrate consensus: Glu"
                     /tool="antismash"
                     /translation="FERQAAFTPERPAIRFSSGSLTYAELDMYAGRLASRLAARGVTKE
                     SIVGVLSERSPEMLIAVLAVLKAGGAYLPLDPAYPKERLSYMLKDSGAALLLAQSGCSA
                     PSFSGEMLEVDMASLASEKAENDVFTPADGCSLAYVIYTSGSTGKPKGVAVEHRQAVSF
                     LTGMQRQFPLQEDDIVMVKTSFSFDASVWQLFWWSLSGASAYLLPPGWEKDPALIVKAI
                     HQEKVTTAHFIPAMLNSFLDQAEIERLSDGTSLKRVFAGGEPLAPHTAARFASLLPQVS
                     LIHGYGPTEATVDAAFYVLDPERDRDRLRIPIGKPVPGARLYVLDAHLAVQPCGVAGEL
                     YIAGAGVARGYLNRPALTEERFLEDPFYPGERMYKTGDLARWLPDGNIEFLGRTDDQVK
                     IR"
     CDS_motif       6835..6876
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0006"
                     /evalue="5.20E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="539"
                     /protein_start="525"
                     /score="19.5"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPLD"
     CDS_motif       7060..7119
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0007"
                     /evalue="2.30E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="620"
                     /protein_start="600"
                     /score="35.3"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGVAVEH"
     CDS_motif       7645..7734
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0008"
                     /evalue="6.40E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="825"
                     /protein_start="795"
                     /score="56.8"
                     /tool="antismash"
                     /translation="PCGVAGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       7816..7851
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOF15_RS21520_0009"
                     /evalue="3.50E-04"
                     /label="NRPS-A_a8"
                     /locus_tag="MOF15_RS21520"
                     /protein_end="864"
                     /protein_start="852"
                     /score="13.4"
                     /tool="antismash"
                     /translation="FLGRTDDQVKIR"
ORIGIN
        1 ctcaaaaacg ggggtcagtc cccttatgca gggcgttttt tcttatataa ctgcgtcaaa
       61 ttgattgact ttccattttt tactttcttt tacaaatgtc actttccgtt tcgctgcagg
      121 tgatccgtct aaagtaggaa cggtaaattc gtatgtgact gtgccgcctt ttttagagat
      181 cagtttagca gttgattttt tccaatttaa aaggttgtca ccgtcgccga ttggcacggc
      241 tagttttcct tttgaaacag tgaagtgata gtctttaaag cctttttcga ttgctgtttt
      301 tgtgaaaata ggagttaagt aattcactgc ttttgcttta gtgcctaggt cactgcacat
      361 gtacacgtat tgaagatttt gatactcaaa agtgcctgat gggcaaactg cttttttcgc
      421 ttttggattg tggccgctca ttgtgttcca aaaatgctct cttgctgata acgccagctg
      481 cagtgcttgt ttttgtgata aatcacctga agtatttttc gcaaatgcat ttgtgcttaa
      541 tgacagaata agtgcaatag ttgttagaaa tagaaaacat tttttcattg tggttcactc
      601 cttcaaaatc tctttctctt atgttacaaa tgttacaata attacggata gattacaata
      661 cttttgcata aattattttt tattctttta ctcacaagaa aataggctgt tacttgctag
      721 tttattcttc ataagctaaa aagatgtgta aaattagaag aaccgggaag ggtttcatat
      781 gtacagtgat gaataataac atgttacata tcctgttttt gtcaaaagaa tagggtcatg
      841 tttacaaacg aattcaaata aaggagcttt caatatggaa acatcacaag aacaacttgt
      901 aactcagaag aaaagcaatc catctgtctg gcggtctatg tcactctttt tagttccgct
      961 tctgctaagc aatgtactgc aatcagtcgg ccagctagtc gggatgatgg ctgtaggcag
     1021 atggcttggt gttgatgcgg ttgcagctgt atcatccttt ttcccgttat tttttctttt
     1081 aatctcattt acaattggga tcggttcagg aagctcaatc ctgatcggac aagcctacgg
     1141 ggcgaaaaat gaggaacggt taaaggctgt tgtcgggacg acgctcacgt ttacgttttt
     1201 attaggagtt gtgttagccg tcatcggcag tattttcacc ttggatattc ttcgtttaat
     1261 gggcacgcct gaaaatgtaa ttcatgtcag cgccagctat gcacgtattt tattttacgc
     1321 gatgccgttt atgtttttat atttcgctta tacaacattt ttgcgcggaa caggggattc
     1381 taagacacct ttttatacgc tgattgtgag cacggtcatt aacatagcgc ttttgcctgt
     1441 cttgatcctt ggcatgttcg gttttccgaa actgggcata tacggctccg catatgcaac
     1501 cgttatttcc acgattgcga cttttattgt gttaatggtg tatttgcgaa aacggaacca
     1561 tccgctgcaa tttgataaaa cagtgcgccg ttatttgaaa atggataaag aacttctggc
     1621 tctcctgctg cgcctcggga ttccatcaag catcaatatg attctcgtct cattatcaga
     1681 gatagcggtt atttcattcg tcaaccatta cggctctaat gcgacagctg catacggcgt
     1741 ggtcaaccag gtagccagct atgtgcagat gccggccgtc agccttggta ttgcggtgtc
     1801 tatattcgcc gcgcaatcga tcggagcgaa cgagtttggc cgcctaaaac aggtaattcg
     1861 tgtcgggatt tggctgaatt atatcattgg cggtgtgctg atcattctga tctatgtttt
     1921 ttcacatcag attttaggat tatttttaac cgaacaagag acgctttaca ttgcgcaccg
     1981 tctcttgatg attacattgt ggagttactt actctttgga aatgcccaaa ttatttctgc
     2041 gacgatgcgg gcaagcggaa cggttttatg gccgaccgtt atcagtattt ttgccatctg
     2101 gggagtagag gtgcctgtcg cgttcgtact ttcccactac acaaatcttg aaatactcgg
     2161 cgtctgggtt ggctatccgg ctgcgtttgc cgtcagcttg ctgttgattt atgggtatta
     2221 tcaattcgtt tggaaaaaga aacagattac acgtctcatc caatagcatc ccgaatcaca
     2281 ccgttcaaca tttgaacggt gttttttgtt tgataaagag aaaacataaa tggaggaaat
     2341 atggcacatt acattgaatt cttatacaag caatgaatca aggagaggtt gagtacacat
     2401 ggcaaacgat attgagaaga ttacgtattt aggaacacct gccatcaaag caggcaacga
     2461 acatatagaa atgattgtcg ttcccgagtg ggggagcaac gttatttctc ttgtggacaa
     2521 aacgacacag cttcagcttt tgcgtgaacc ggaaacagca gaaagctttc atgatacacc
     2581 aacattgtat ggcattccga tcctgtttcc gcctaaccgg ataagtgacg gtacattcag
     2641 tttccggggc agaacatatc attttgatat caatgaaaag gataaacaca accatcttca
     2701 tgggtttctt tatcaggaaa aatggaacgt agtgaccatg aagcaaactg atgaagaagt
     2761 gatcgttgaa actgaaattg acctttcaga gcttccccat gtgtataagc aatttccgca
     2821 tcatgccgtc gttcggatga catacacgct aaaggggaat actctattta aacatgcgac
     2881 ggtcatgaac aatggaaagg aagcgtttcc atggggaatc ggctatcata ctacatttgt
     2941 ttttccagaa gaaagctcgt tattctccct gacagcagat cagcaatggg agttggatga
     3001 gcgcctgctg ccgaccggga aactgatgga tgtaccgaat aaagaagcct tgcatgaagg
     3061 gatggattta cgcagccagc agctggatga tgtcttttta tcttcttatc agaaaagagg
     3121 tggagaaaat cagacagtca tttatcatca gcatgcacat ataagcatta tttataaggc
     3181 agatgaacaa tttaaacatt gggttgtata caacgcggac ggaaggcagg gatatttatg
     3241 ccctgaacct tacacatggg tgacaaacgc tgtgaatctt gatgtgtctc catcactcac
     3301 tggtgtacaa gtgctggagc cgggagaaga aacgacagcc aaaagcagta tcaccattga
     3361 actgaatcat caatagccca tgatgaggcg cagagaggcg gggtaaaaaa atgaaaaaaa
     3421 gcataaagct ttatgttgct gttttgctgc ttttcgtcgt tgcttcggta ccttatatgc
     3481 atcaggctgc gcaagcggcc gaaaaacaag gtgcacttgc cgggcagatc gataagattt
     3541 taactgatca tcctgcactc gaaggcgcaa tggccagcat caccgttcgc tctgccgaaa
     3601 caggcgctgt tctatatgag cattcaggag acatccgaat gaggcccgcc tcttctttaa
     3661 aacttttgac agcggccgca tcactttcta ttctcggaga ggactattcc tttacaaccg
     3721 aggtccggac ggacggcaaa tttaaaggga aaaagctgaa tggaaacctt tatttgatag
     3781 ggaaaggaga tccgacactt ttaccatctg attttgaaga aatggctgag aaattgaaac
     3841 aatccggcgt gaaagagatc aaaggcaatc tgatcggtga tgatacgtgg tatgatgaca
     3901 tccgattatc gcctgatatg ccgtggagtg atgaatacac atattacggc gcgcaaatct
     3961 ccgctttaac agcgtctccg aatgaagatt atgatgcagg cactgtgatt gtagagatca
     4021 ccccgggtaa aaaagagggg gaaaagcctg ccgtttctgt atctccgaag acggattatg
     4081 taacgattaa gaacgatgcc gaaacgactg cggctggcca aaagaaagac ctcacgatca
     4141 aaagagagca tggaacaaac acgatcacaa ttgaagggag cgtaccggtt gacgcaagca
     4201 agacaaagga atggattgct gtctgggagc cggctggata cgctttggat ttatttaagc
     4261 aggcactaaa aaaacaagga attactgtaa aagggcacgt caagacaggc actgcgcccg
     4321 gttcttcaga cgtgttaatc tctcatcact caatgcctct gtcaaaacta tttgtgccgt
     4381 ttatgaaatt aagcaataac ggacatgccg aagtgctcgt aaaagagatg gggaaagtga
     4441 aaaaagaaga aggaagctgg gaaaagggac ttgaagtatt aaacaatgcg cttccggaat
     4501 ttggtgttga tcccaaatca cttgtcttaa gagacggatc aggtgtctcc catattaatg
     4561 ctgtatcctc agatcaaatc tcacagctgt tatatgacat tcaagatgag aaatggttct
     4621 cagcttatct aaattctctg ccggttgcgg gcaatagtga caggatgata ggcggaacac
     4681 tgaggaaccg gatgaaagac actcctgcac aaggaaaggt aagggcaaaa accggatcgt
     4741 taagcacagt cagctcttta tccgggtacg cagaaaccaa gagcggaaaa acccttgttt
     4801 tttctattct tttgaacgga ttgattgatg acgaggatgg aaaagatatc gaggatcaaa
     4861 tcgctgtcat cttggcaaat caatcatagc gtcagacctg ttttccaagg aaaacaggtt
     4921 tttttatgtc ttcaataata ttccggtcgc atctgccaac aaatagaaag attacgcgca
     4981 acagcaaatt agtacaactg aaaaattagt gtgtaagacc cgaatcagaa tgatactttt
     5041 atcactttat gttcaaaaat ttgatgaaga aagaaaccaa aactaaagaa ataatactat
     5101 attcaaaaaa atgttataaa aatttaatat tttttattgt actttaaaaa atatccgtta
     5161 atatagtgcg tatatggatt atatagtcat ataatttctt ttattgtcat ttttccactt
     5221 ttttcttact ctcttattat gagaactgga gggaatccgt tgagccaaca tacttattct
     5281 ttaacccatg cccaaaggag agtgtggttt accgaactgc tggagccagg taccagcatt
     5341 tgcaatctta cggcctgtgt gaaattcaga ggcaatattg atcttgatgt tctagagggc
     5401 gcgctgaatc attccatctc ccgcaacgac gccatcaggt ttcagctgct agaaggagaa
     5461 gagcttgagc cgcgattgta ccttactgaa tataaatata cttcattggg aataatagat
     5521 ttttcaaatg ttgaaatgat agaaatagag caatggatac aagaacaagc gagaatccca
     5581 tttaaactat tcaactcgcc tctttatcaa ttttatctcc ttagaatcca caatcatgag
     5641 gtttggctgt ttgctaaatt ccatcatatt ataatggacg gcatctcctt aaacgtgatg
     5701 ggaaatcaaa ttatagatct ttatcaaaaa atgaaaaagc aagatccttt acccgatcag
     5761 cctgaacctt cttacttgag ctacatcgaa aaagagagtc aatatttaca gtcttcgcgt
     5821 tttacaaagg accgtttatt ttggactcaa acctttcaga atccgccggg ataccactcg
     5881 ttagccggcc agacgtcttt acaaaaacag agcacatccg catccagaga caccatcacc
     5941 ctttcacctc atctagggca aacgattcga atcttttgcg aagaacaaaa gatcaatatc
     6001 atttccttat ttatggcttc attttacata tgcatcagcc ggatcacttc taaaaaagac
     6061 ctttccattg gcacctacta tggcaacaga gggtcaaagg ctgaaaaaga aatgctcggc
     6121 atgttcgtca gttctcttcc catcagaata acggttgatc ctgacgcgga ctttctatcc
     6181 tttgtccgca gagtaggcag ggaacagctt tccgttatgc gtcaccaacg gtttccttat
     6241 aatttgcttg taaatgaatt aagaaaagaa cagaaggatt tacacaacct tatcggtata
     6301 tctatgcagt atcaaccgct ccaatggcac aatgcagacg gttttgacta tgaaacggcg
     6361 ctttatttca gcggctacac cgcgaacgag ctttctgttc aaatacagga acggacagac
     6421 accggaacta ttcaattaaa tttcgactat caaaatacct tgttttctct tgaggatatt
     6481 aagcgaatac agaaccatct gcttacgtta ctggaaaacg cccttcaaca tccgcacagt
     6541 ttcataaaag aattagatat gactaatata agagagaaac aaaaattgtt gtatgagttt
     6601 aataagacgg aagctgtttc gcctgaagcc ccgaccctgc acggactgtt tgaacgacag
     6661 gcggctttca caccggagcg gccagccatc cgcttttcca gcggttcgct gacgtacgct
     6721 gagcttgaca tgtatgccgg ccggctggcc tcgcgcctcg cggcacgcgg cgtcacgaag
     6781 gaaagcatcg tcggtgtcct gtctgaacgg tcacctgaaa tgctgatagc tgtacttgct
     6841 gtcttgaagg caggcggggc gtatttgccg cttgatcctg catatccaaa ggagcggctg
     6901 agctacatgc tgaaagacag cggggccgcg ctcttgctgg cacagtcggg atgttccgca
     6961 ccgagctttt ccggagagat gcttgaagtg gacatggcat ctcttgcgag cgaaaaagca
     7021 gaaaacgatg tgtttactcc tgctgacggc tgttctctcg cctatgtaat ctatacatcg
     7081 ggctccaccg ggaagccaaa aggggtagcc gtcgagcacc gccaggctgt ttcctttctg
     7141 actggcatgc agcgtcagtt tccgcttcag gaagatgaca ttgttatggt gaaaacctct
     7201 ttttccttcg atgcgtctgt gtggcagctg ttttggtggt ccctttccgg cgcttcggct
     7261 tatctgcttc cgcccggctg ggagaaagac ccggcattga ttgtaaaagc cattcatcag
     7321 gaaaaagtga caacggctca ttttattccg gctatgctga acagctttct cgatcaagcg
     7381 gaaatcgaaa ggctgagtga cgggacaagc ctgaagcgtg tattcgccgg aggtgaaccg
     7441 cttgcgcccc atacggcagc ccgttttgct tctttattgc cgcaggtatc gctgattcat
     7501 ggctacgggc cgacagaagc gacggttgac gcggcatttt acgtgttgga cccagagcgg
     7561 gacagggatc gcttgcggat tccgatcggg aagcccgtac cgggtgcgcg tttgtatgtt
     7621 ttagatgcgc atttagccgt acagccttgc ggtgtcgccg gcgaactgta catagccgga
     7681 gcgggtgtgg ccagaggcta tttgaatcgg ccggccttaa cggaggagcg ttttctagag
     7741 gacccgtttt acccgggtga acgcatgtac aaaaccgggg atttggcgcg ctggctccct
     7801 gacgggaata ttgaattcct cggccgcacg gatgatcagg tgaaaatccg cg
//
