LOCUS       NZ_JALAXV010000017     10366 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain T18B2 contig_0000017, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAXV010000017
VERSION     NZ_JALAXV010000017.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 232839)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 232839)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 556.3003030959693x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:40:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,343
            CDSs (total)                      :: 4,261
            Genes (coding)                    :: 4,152
            CDSs (with protein)               :: 4,152
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 71
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 74 of 109
            Pseudo Genes (incomplete)         :: 58 of 109
            Pseudo Genes (internal stop)      :: 22 of 109
            Pseudo Genes (multiple problems)  :: 35 of 109
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:22:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 125737
            Orig. end                         :: 136103
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAXV010000017.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10366
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10366
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10366
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5366](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5366
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            779..1261
                     /locus_tag="MOF15_RS20140"
                     /old_locus_tag="MOF15_20095"
     CDS             779..1261
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258635.1"
                     /locus_tag="MOF15_RS20140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20095"
                     /product="GyrI-like domain-containing protein"
                     /protein_id="WP_060398811.1"
                     /transl_table=11
                     /translation="MSFSHITHLKQKRFAGLSQRTSNALEMTEERRIPALWDQFWQQDM
                     SILFSQAEKDQSIIALYSNYEQETNGFYTFSVGAFQEDSGTLPEPYENIDLPASAYAVF
                     TSRIGPIEEIVYETWKEIWTWDKRHLRTFTGDFEMYDQSAAVPQRAQVNIYVAIKN"
     gene            1338..2039
                     /locus_tag="MOF15_RS20145"
                     /old_locus_tag="MOF15_20100"
     CDS             1338..2039
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389789.1"
                     /locus_tag="MOF15_RS20145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20100"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_060398810.1"
                     /transl_table=11
                     /translation="MRVTQNQTVWQLTFFPVLFPVNCYLVEEENEVTLVDAAIPGSYKG
                     IIQAVNQLGKPLQHILLTHAHGDHVGSLDILTQAFPHAKVMISERDSFLLQGDTSLRHD
                     EPQTPIKGGIPKHVQTKPHHLLTGGETIGSLLAIPTPGHTPGSMSFLDTRNGIIIAGDA
                     FQLRGGIAVSGQVKWTFPFPAFATWNKEEAIKSAQLLADKAPSCLAVGHGNFLRSPSER
                     MRQAIQKAKKG"
     gene            2044..2619
                     /locus_tag="MOF15_RS20150"
                     /old_locus_tag="MOF15_20105"
     CDS             2044..2619
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389788.1"
                     /locus_tag="MOF15_RS20150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20105"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_268284355.1"
                     /transl_table=11
                     /translation="MSPRIGLNQKMIVDAAAEIADQEGLNSVTLAALSKKMNVRPPSLY
                     NHVNGLQAIRTELAVNGLTKLFDQMAASVTEQKGDAALLSLAHAYVDFAVENPGYYEAA
                     LLKVQDNRAEIVSDQIVQLVTNLLIENRYACEKTAIHATRGLRSLLHGFTALIAKEAFE
                     REEDILESLSFSIRTFLSGLSINNKNVM"
     gene            2697..3440
                     /locus_tag="MOF15_RS20155"
                     /old_locus_tag="MOF15_20110"
     CDS             2697..3440
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389787.1"
                     /locus_tag="MOF15_RS20155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20110"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_268284356.1"
                     /transl_table=11
                     /translation="MVDIHKIEQLAAASWPSYIQKSMGQWLLRANFGVTKRANSVWTSA
                     DMPEGEFQQEAEHFYKSLGLPVCFHISNASPKGLDGALADSGYEKADECFQMTALCRDI
                     MNRTEDNRRLTYKWEQVPSSVWIDNFIRLEGFSPKRHQGYSNIFKRMPPCKTFFTMYDQ
                     GSLTAVGTASVIEGYGGLSNIVVAKKHRGKGAGTQVIRVLTEWAISNGAKHMYLQVLKE
                     NLAAVSLYEKIGFSPISEHHYRIKK"
     gene            3455..4180
                     /locus_tag="MOF15_RS20160"
                     /old_locus_tag="MOF15_20115"
     CDS             3455..4180
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389786.1"
                     /locus_tag="MOF15_RS20160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20115"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_268284409.1"
                     /transl_table=11
                     /translation="MLMDSLQEIICEKRTYTRLYHSHKHAYSQFLFQLEGSIDLETEGR
                     EVTLKPDRFLYIPAECEHRFRSIGRNECLVLDVPLYALKIDKFHEGSGIEAALDPFWSS
                     IRYLLTEEAKAGTANSLHMLVQYIKEKLQSDTCASIAYIHSHLSERLTIKKLAEIEHYH
                     PAYYSSWFKKQTGKSPQSYIANLRLEEAKRMLMEKKETLTNVSEALGFQNLSSFTRWFT
                     KGTGMPPRLYRNTLYSDKK"
     gene            4245..4577
                     /gene="csaA"
                     /locus_tag="MOF15_RS20165"
                     /old_locus_tag="MOF15_20120"
     CDS             4245..4577
                     /codon_start=1
                     /gene="csaA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389785.1"
                     /locus_tag="MOF15_RS20165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20120"
                     /product="chaperone CsaA"
                     /protein_id="WP_268284357.1"
                     /transl_table=11
                     /translation="MAVIEDFEELDIRTGTIVKAEEFPEARVPAIKLLIDFGVEIGMKQ
                     SSAQITKRYQPEDLINKQIIAVVNFPPRRIAGFKSEVLVLGGIPSQGDVVLLQPDQPVP
                     NGTKIG"
     misc_feature    complement(4865..4880)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            5001..5366
                     /locus_tag="MOF15_RS20170"
                     /old_locus_tag="MOF15_20125"
     CDS             5001..5366
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="MOF15_RS20170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20125"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_268284358.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.2e-07,
                     bitscore: 25.5, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKVFSSMVLGATLLVSSSVIFASSSDEATHGEVNLDETRDLIQ
                     IMKLTKTKAGGGTWYHGFEDGQVKSNYNHTKKTHKSSAKAGGERFFSSKWKKKDEGYTY
                     ASVYQTLWGNRAFWDTK"
     gene            5460..7631
                     /locus_tag="MOF15_RS20175"
                     /old_locus_tag="MOF15_20130"
     CDS             5460..7631
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="MOF15_RS20175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20130"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_268284359.1"
                     /transl_table=11
                     /translation="MKKTIYILFILFLLSMNISSYHYTLNKQVFYTLFSNKQTLIINYN
                     KAHFKSDDFIKGISGFSKKENVNITQYNFLDEKTLNIYASNLKNEPSIKIGDRFISNFN
                     KTANESDKNLIGTIDFPLSTWKIRYFNLNQISNVGLGNRFYISSEDEDVIEQAKKLFSD
                     YGDVSIEENISLNDSAVFSTALLLLALLSMLILFIGILYFVIKNRKQLLLQKLWGYSKT
                     KSLLTFPKMFLKPLVMIIICGALLTVILAFIFNLNDWLSVYVKMYMRNALIVTFSMMVY
                     TIIVTLILYNNSNISATIKGGIPFKKFQWLSLGFKLIITILLFNIFASSLSNLFDLKQR
                     LDNQSYWNKTQTVYKTSFADTGLNYSDLKIDREKNEKVRKLYHELERHKDAFIMDAQNY
                     GILKMNGKTPVYFYTLNTTKENEIYSPSGRSVTISPNYLKVNTIKGINGKTITEKQLTF
                     DQNTLNLLVPKKYAKYKEKILKAYKNQFFFDKVEVDNMYNKEIQQPLNTLKKERLKIKI
                     IYTQNNQEYFTYNSMLGNNKNNIVDPIAIVFTDNVDSSVIGAYATSSLFFSDASKGMAY
                     DHIIPYLDKTDTKELIGSAISVYQELSDQIARIQNQFIQNLIGLTITLILSVAFLISYI
                     WSYYSANAYRLYLKEIFGYSYWARNKSLIILSTVSNCLIGICFSIYYKIYELIGLIVLF
                     FGIEMIILYFLSLYLNRKNMNKILKGDRL"
     misc_feature    5465..5476
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.95"
                     /tool="antismash"
     gene            7628..8248
                     /locus_tag="MOF15_RS20180"
                     /old_locus_tag="MOF15_20135"
     CDS             7628..8248
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328395.1"
                     /locus_tag="MOF15_RS20180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20135"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_268280435.1"
                     /transl_table=11
                     /translation="MIELKSISKSFDGKSVLSNFSIHIEENEFVSIVGKSGSGKTTLLN
                     IISLLDSPDEGEIEILGYKNPKSKEVMKLRRENLGYIFQNYVLMDNETVLTNLLLSTAY
                     AKDFDKNKLSEALEMVGLEKSFLKKKVYQLSGGEQQRVAIARIILKPCDIILADEPTGN
                     LDEYNKNIILSLFHQLKEMGKTIICVTHDPEIANCSDRVINLS"
     gene            8262..8474
                     /locus_tag="MOF15_RS20185"
                     /old_locus_tag="MOF15_20140"
     CDS             8262..8474
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328394.1"
                     /locus_tag="MOF15_RS20185"
                     /note="Built to rescue LMOF2365_14255 during structural
                     annotation change regression.; Built to rescue
                     LMOF2365_14255 during structural annotation change
                     regression; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20140"
                     /product="bacteriocin-like WGxF protein"
                     /protein_id="WP_268280436.1"
                     /transl_table=11
                     /translation="MKHITFAISTSILIIITGIVHRVIFRLFGFPFDEALFFWGGFVVV
                     YFVIALIASLLFRNVGSSSSRRYQA"
     gene            complement(8622..9134)
                     /locus_tag="MOF15_RS20190"
                     /old_locus_tag="MOF15_20145"
     CDS             complement(8622..9134)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MOF15_RS20190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MOF15_20145"
                     /product="hypothetical protein"
                     /protein_id="WP_268284360.1"
                     /transl_table=11
                     /translation="MKKTVLSTLSLGLLFSTVAPLTSHAESISEPTPSYLTQVNSTPLQ
                     ELIKTSSVPVIQENGKISIQAVPPSTKWKVVDHFRGNSKNIDTAAELGARIAAGLPGLK
                     YSWAGIFAQAVFGTFYHKKPTRYYSTTQYLAYDKKYRYTIVDMYVYKDSKYSKQIGYDY
                     KVFRTPR"
     gene            9229..9471
                     /locus_tag="MOF15_RS20195"
                     /old_locus_tag="MOF15_20150"
     CDS             9229..9471
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MOF15_RS20195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MOF15_20150"
                     /product="hypothetical protein"
                     /protein_id="WP_268284361.1"
                     /transl_table=11
                     /translation="MNFDDKKSILKTVGNVFMVLVLISLFVYAFSNESIGWYVKITSIV
                     MLTISYICYALISLQEKWFILTLIISWLVIIFWVF"
     gene            9601..10089
                     /locus_tag="MOF15_RS20200"
                     /old_locus_tag="MOF15_20155"
     CDS             9601..10089
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003221134.1"
                     /locus_tag="MOF15_RS20200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF15_20155"
                     /product="hypothetical protein"
                     /protein_id="WP_268284362.1"
                     /transl_table=11
                     /translation="MWKALRQLLKNQKNQSPLDEGYLQIPKKDINLELKQKIFELESLG
                     EVVSKKLQNKYIDVFEAQGKPNLTIKICRDIDGDELNDLTAERCLEKEYRSQIAINFYQ
                     PAKAYDEDLDCITMDLWYYYGGFVGIGCGTLFDLNNNDLEKEIEEVLIFLLNDKQSEN"
ORIGIN
        1 tgaaagagga ggagctgttt gcgattgcgt ccgctttgca gagtgtctct tccatgtgtg
       61 aacctgcttc tcacagcaca gcttatcaaa aaattaagca cctcataccg gagcaatctg
      121 ctgaagcatt taagcatcaa acagaaaaat ggtttattga catgacggct tggggccata
      181 ctgaggatca aaaggcactt cgtgagaaaa tcagtgccgc catcgatcaa ttgtcaacga
      241 tatcatttac atatacgaac gcaagcgggg aaacacttct tcgtgaaaca gaaccctaca
      301 cattggtttg caaggcggga cactggtatt tatacgccta ttgtctggtt agaaacgatt
      361 ttcgcttttt taaacttaac cggatgaaag atatggccat tttacaaaat tcctttatca
      421 gaaaagacat tcagctggat accttgccgt gggataggaa ttggtacgaa aaggaccgtt
      481 taactgaact ggttattctt gttcagcagc cagcacgtca gcgaatacgt gaatggttcg
      541 gatctgaagt tcttcattgt gatgaagatg agaactgccg ggcagtgatt tctcttccag
      601 aagatcaatg gctgatcggt tttttgctgc agttcgggaa ggatattgag gttttggaac
      661 cccttcatat acgggataaa gtaaaagata ccattcatca gatgcaaaaa gtatatgaaa
      721 cctgacacac tgctgtcagg tttctttttt tataatctag gtaaaaagga gggaacatat
      781 gagtttttca catataacac acttaaaaca gaaacggttt gcaggactgt cacagcggac
      841 aagcaacgcg ttggaaatga cagaagaaag gcggatcccg gccctatggg accaattctg
      901 gcagcaggat atgagcattc ttttctcaca ggctgaaaaa gatcaaagca ttatcgcctt
      961 atattccaat tacgaacagg agacgaatgg gttttacaca ttttcagtcg gagcgtttca
     1021 ggaggacagc gggactcttc cggaaccgta tgaaaacatt gatttgccgg cttccgctta
     1081 tgccgttttc acttccagaa tcggccctat tgaagagatt gtgtatgaaa cgtggaagga
     1141 aatttggact tgggataagc gtcatctgcg gacatttaca ggcgactttg agatgtatga
     1201 tcaaagtgca gcagtacctc aacgggcgca agttaatata tatgtcgcca taaaaaattg
     1261 ataaactctt gaaaaactaa tggtgttagt tataatgaaa ctaataccat tagttttttg
     1321 ttagggggaa taaaaagatg agagttaccc agaatcaaac ggtttggcag ctgacttttt
     1381 ttccagtttt gtttccggtc aattgctatt tagtagagga agaaaacgaa gtcacccttg
     1441 ttgatgctgc aattccaggc agctataaag gaattattca agctgtaaat cagctgggaa
     1501 aaccgcttca gcatatttta ttgacccatg cacatgggga tcatgtcgga tcactggata
     1561 tattgacaca agcgtttcct catgcaaagg tgatgatctc agaacgggat tcttttctgc
     1621 tgcaaggtga tacatccttg cgtcatgatg agccgcagac acctataaaa gggggtattc
     1681 cgaaacacgt gcaaacaaag cctcaccatc tgctgacggg cggtgaaaca attggttctc
     1741 tgcttgcaat tccaacaccg ggtcatacac cgggcagcat gtcatttctt gatacaagaa
     1801 acggcattat aatagcagga gatgcatttc aattacgcgg aggaatcgct gtatcaggcc
     1861 aggtaaaatg gacgtttcct tttcctgcct tcgccacatg gaataaggag gaggccatca
     1921 aaagcgcaca attgctcgcc gacaaggcgc cttcctgtct cgctgtcgga catgggaatt
     1981 ttctgagatc accctcagag cggatgcgac aggcgataca aaaagcaaag aaaggatgat
     2041 agcatgtcac ccagaatagg attgaaccaa aaaatgattg tagacgcggc agcggagatt
     2101 gccgatcagg aaggcttgaa tagtgtcacg cttgccgcac tgtctaaaaa aatgaatgtt
     2161 cgccctccat cgctgtataa tcatgtcaac gggctgcaag caatcagaac agagcttgct
     2221 gtcaacgggc tgacaaagct ttttgatcaa atggcagcct ccgtaaccga acaaaaggga
     2281 gatgccgctc tgctttcttt agctcatgca tatgttgatt ttgcagttga aaatcccgga
     2341 tattatgagg cggctttgct taaggttcag gacaacaggg cggaaatcgt gtcagaccaa
     2401 attgtccagc ttgtaacgaa tctgctcatt gaaaatagat atgcatgcga aaagactgcc
     2461 attcacgcga caagggggct cagaagtttg cttcatggct ttactgcttt aatcgcaaaa
     2521 gaagcatttg aacgtgaaga ggatatttta gaaagtctgt cattttcaat tcgaacgttt
     2581 ttgtcaggat tgtcgattaa caataaaaat gttatgtaaa ctaataaata gatgtcttat
     2641 atttctgaat attgtataat gtcatggtag tatacatagg ggagagacat tatgctatgg
     2701 ttgacattca caaaattgag cagttagcgg cagcttcatg gccgtcttat attcagaaaa
     2761 gcatgggaca atggcttctc agagccaact ttggcgtaac caaaagggcg aacagcgtat
     2821 ggacttctgc ggatatgcct gaaggagaat tccagcaaga ggccgagcat ttttataaaa
     2881 gcttaggctt gcctgtttgt tttcatatca gtaatgcatc tccgaaaggg cttgatggcg
     2941 cattggcaga cagcggatat gaaaaggcag atgaatgttt tcaaatgact gcattgtgcc
     3001 gggacattat gaaccgtaca gaagacaatc gccgattgac atacaagtgg gaacaggtac
     3061 cgtcgtctgt ttggattgat aattttataa gattagaagg attttcgccg aagcggcatc
     3121 aaggctattc aaacattttc aaacgcatgc cgccgtgtaa aacctttttc acgatgtatg
     3181 accaagggag cctgacagcg gtgggaacgg cttctgtcat tgaaggctac gggggactca
     3241 gtaacatcgt tgtagccaag aagcatagag ggaaaggggc aggaactcaa gtaatcaggg
     3301 tgttgactga atgggcaatc agcaacggag cgaagcacat gtatctgcaa gtgctgaaag
     3361 aaaaccttgc ggcagtttct ttatatgaaa aaatcggatt ttcacctata tcggaacacc
     3421 attaccggat caaaaaatga ccaaaggaag gggcttgctg atggactcgc ttcaagagat
     3481 tatttgcgaa aaacggacat atacacgcct ttatcattca cataagcacg cgtacagcca
     3541 atttcttttt cagcttgaag ggagtattga tcttgagacg gaggggcgcg aggtgacatt
     3601 aaagccggat cggtttttat atattccggc tgagtgcgag catcgtttcc gttcaatcgg
     3661 gagaaatgaa tgtcttgtac tggatgttcc gctatatgcg ttgaaaattg ataaatttca
     3721 tgaagggtcg ggaattgaag ctgcacttga tcctttctgg tcatcaatca ggtatttgct
     3781 gacggaggaa gcaaaggccg gcacagcaaa ctcgctgcat atgcttgttc aatacataaa
     3841 agaaaagctg caatccgata cctgcgcctc aattgcatac attcacagcc atttatctga
     3901 gcggctgacg attaaaaagc tggctgaaat cgagcattac catccagctt actattcaag
     3961 ctggttcaag aagcagaccg ggaagtctcc gcagagctat attgccaatc ttcgtctcga
     4021 ggaagcgaaa cgaatgctga tggagaaaaa ggaaaccctt actaacgtga gcgaggcgct
     4081 gggctttcaa aatctttctt catttacaag atggttcacg aaaggtacag gcatgccgcc
     4141 ccggctatat cgaaatacct tatattcgga taaaaaatga tggtcctttg gttaaacaaa
     4201 cctcatacac ctcttttata ataggagaaa aaggagttat tgatatggca gtcattgaag
     4261 actttgagga attggatatc aggacaggca ctattgtaaa agcggaggaa tttcctgaag
     4321 caagagtccc ggccattaag cttttgattg attttggagt agaaatcggc atgaaacaat
     4381 cgagcgcgca aatcacgaag cgttatcagc ccgaagatct catcaacaag caaatcattg
     4441 cagtcgtcaa ctttccgcct cgccggatcg cgggatttaa atcggaagtt ttggtgctcg
     4501 gcggcattcc cagccagggc gatgtcgtcc tattgcagcc ggatcagccc gtcccaaacg
     4561 gcacaaaaat cggataaaca aaaaagacgt ttgccttagg caaacgtctt tgcgtattga
     4621 ggcgatgcac cacacgccac attcgtgtgt gccgcaaagg gcatgtacat cggcttacac
     4681 aattcagctc atcgctatat gaatatagca tggcggctga aggaatgcaa gggtaacttt
     4741 ttaattgtga gtgaaatagt tataaaagct tgtcttattt tgtctaaatt aatcgtgagt
     4801 aattcaaata ttactgtatg atgattgttc gacaataatg agtttgtcaa caataacacg
     4861 gaatgtgact taactgtgac ctcaaacacc attttaagaa taggcagaaa tgcctttaaa
     4921 caaacgaaaa cgtacttaat ggtgaataca aggggtaact gtgacctggt ttcttgtgaa
     4981 aacaaaaagg agaggttata ttgaaaaaga aagttttttc ttcaatggtg ttgggagcta
     5041 ctttactagt atcatcttct gtcatttttg ctagttcttc agatgaggct acacacggag
     5101 aagtgaactt ggatgaaaca agggatttaa tacaaatcat gaaattgacc aaaaccaaag
     5161 cgggtggcgg tacttggtat cacggtttcg aagatgggca agttaaatcc aattacaatc
     5221 atactaaaaa gactcacaaa tcaagtgcga aggctggtgg agaaaggttc ttttcatcta
     5281 aatggaagaa aaaagatgaa gggtatacct acgcttcagt gtatcaaact ctttggggaa
     5341 atagggcttt ttgggatacg aaataaaaca gacaataaca aagtgatttt taatctatct
     5401 tccggtgccg tcaaaacttg aatataaggc accggatttc tttttatgga ggctatccaa
     5461 tgaagaaaac gatttatata ttgttcattc tatttttact ctcaatgaac atatccagct
     5521 atcactatac tctgaataag caagtttttt atactttatt ctctaataaa caaacgttaa
     5581 ttattaatta caacaaagca cactttaaaa gtgatgattt cattaaggga atatctggat
     5641 tttcaaaaaa agagaacgtg aatattactc aatacaattt tttagacgag aagaccttaa
     5701 atatctacgc ttcaaacctt aagaatgaac cttcaatcaa aataggagat aggtttatat
     5761 ctaatttcaa taaaacagca aatgaatcag ataaaaattt gattggtact attgattttc
     5821 ctttatcaac atggaaaatc agatacttca atttaaacca aataagcaac gtagggttag
     5881 gtaatagatt ttatatttca tccgaggacg aagatgtaat tgaacaagct aaaaagctat
     5941 tctcagacta tggtgatgtc agtattgaag aaaacatcag tttaaatgac tctgctgtgt
     6001 tcagcaccgc attgctcttg ttagctttgc tttccatgct aatcttattt attggcatcc
     6061 tttatttcgt aattaaaaat cgcaagcaac ttctcttaca gaagctatgg ggatattcga
     6121 aaaccaaatc actgttaact ttcccaaaaa tgtttttaaa accacttgtt atgatcataa
     6181 tttgcggcgc ccttctcact gtaattctgg cttttatatt caacctaaac gactggttat
     6241 cagtgtacgt taaaatgtac atgcgaaatg cactgattgt gacattctca atgatggtat
     6301 acacaattat tgttactttg attttatata acaacagcaa tataagcgcc acgataaaag
     6361 gcggtatacc atttaaaaag tttcaatggc tgtcattggg ttttaagtta attataacaa
     6421 tcctactttt taatattttt gcttcatccc tctctaattt gttcgattta aaacaacggc
     6481 tagacaatca atcttattgg aataagacac aaacagttta taaaaccagt tttgctgata
     6541 caggtttaaa ctacagtgac ttaaaaattg atagagaaaa aaacgagaag gtacgaaagc
     6601 tttatcatga attagaaaga cataaagatg cgtttataat ggatgctcaa aactatggaa
     6661 tactgaaaat gaatgggaaa actccagttt atttctatac attgaataca accaaagaaa
     6721 atgaaattta ttcaccatcc ggaagaagcg taacgattag tcctaattat ttaaaagtaa
     6781 acaccattaa aggaattaac ggaaaaacga ttactgaaaa acaattaacc ttcgatcaaa
     6841 acacattgaa tttacttgtt ccaaaaaaat acgctaagta taaagaaaaa atcctcaaag
     6901 cttacaaaaa tcaatttttc tttgataagg ttgaagtaga caatatgtat aacaaagaaa
     6961 ttcagcaacc attgaatacg ctgaaaaaag agcggttgaa aatcaaaata atatatactc
     7021 aaaataatca agaatacttc acttataata gtatgctcgg caacaataaa aacaatattg
     7081 ttgatccaat tgcaattgta ttcactgata atgttgattc atcagttata ggtgcatatg
     7141 caacctcaag tttattcttt tctgatgcct caaaaggtat ggcatatgac cacatcatcc
     7201 cttatttaga taaaacagat acaaaagagc tgattggttc tgcgatatca gtctatcaag
     7261 aattaagcga ccaaatagca cgaattcaaa atcagttcat ccagaatcta attggattaa
     7321 ctataacact catattgtca gtagccttcc ttatctctta tatatggtca tactacagtg
     7381 caaatgcgta tcgactgtac cttaaagaga tatttggata ttcatactgg gctagaaata
     7441 aaagtttaat catattgtcg actgtgtcta actgtctgat aggaatctgc tttagtattt
     7501 attataaaat ctatgaatta ataggcttga ttgtattgtt ttttggaatt gaaatgatta
     7561 ttctgtattt cctcagcctt tatttaaaca ggaaaaacat gaataagatt ttaaaaggag
     7621 acagattatg atagaactta aaagcatatc caaaagtttt gatggaaaat cagttctttc
     7681 taacttttca attcacatag aggaaaatga attcgtttct attgttggaa agagcggttc
     7741 tggcaaaacc actcttctaa atataatcag ccttttagat tctccagatg aaggcgaaat
     7801 tgagatttta ggatataaaa atcctaaatc taaagaggtg atgaagttaa ggagagagaa
     7861 tttaggatat atctttcaaa actatgtgct catggataat gaaactgtat taacaaattt
     7921 attgctttcc acagcatacg caaaagactt tgataagaac aagctgtcag aagctctaga
     7981 gatggtcggc ttagaaaaaa gctttttaaa gaaaaaggta taccagttga gcggtggtga
     8041 gcaacagcgt gtagcaatag ccaggattat tctaaaaccc tgcgatatca ttctggccga
     8101 tgaaccaacc ggaaaccttg atgagtacaa caagaacatt attctttctc ttttccatca
     8161 gttgaaagaa atggggaaaa caatcatatg tgtaacacat gatccagaaa tagctaactg
     8221 ctctgataga gtaataaatc tttcgtaagg agttgttctt gatgaaacat atcacttttg
     8281 caatttctac aagcatcctg attattatta ccggtattgt tcacagggtt atttttagac
     8341 tatttggatt tccattcgat gaagcgcttt tcttctgggg cggatttgtt gttgtttatt
     8401 ttgttatagc tttgattgct tccttactct ttagaaatgt tggttcttct agcagtagga
     8461 gatatcaggc gtaactcaat ctggtcatta aacaataaaa tataccttat cttgaataca
     8521 tcccaaatat aaagtatcct gtatatgtat caaagttggt gtatcaacca ctataggcca
     8581 gagagttccc tcacatactc tctggctttt tatctgacat attatctagg agttctgaaa
     8641 actttataat cataacctat ttgtttgcta tatttactgt ctttgtagac gtacatatca
     8701 acgattgtgt atctatactt tttgtcgtag gctaagtatt gggttgttga gtaataacga
     8761 gttggttttt tatgatagaa tgttccaaac acagcttgag cgaatatacc agcccaggag
     8821 tatttaagac caggtaaccc tgcagcaatt cttgctccta attcagctgc agtgtctatg
     8881 ttcttagagt tccctctaaa atgatctaca accttccatt ttgttgatgg tggcacagct
     8941 tgaattgaaa tttttccatt ctcttgaatg actggaactg atgatgtttt aattaattct
     9001 tgaagtggtg ttgaattgac ctgagtgaga taacttggtg taggttctga gatggattct
     9061 gcatggctag ttagtggagc cactgttgag aataggagtc cgagtgatag ggtagataga
     9121 actgtttttt tcacttcaca atcatccttt caagtttttt aatgcttatg actgtctgga
     9181 tgctgtataa ttataacata taatatacat attttggagg ttgaaattat gaactttgat
     9241 gacaaaaaga gtatattaaa gactgtagga aacgtattta tggttcttgt tcttatctct
     9301 ttatttgttt atgcattctc caatgaatct attggatggt atgttaagat cacttctatt
     9361 gtgatgttaa ctatctctta tatttgctac gctttaattt ctttgcagga aaaatggttc
     9421 attttaactt tgattatctc ctggcttgtg attatttttt gggtattcta aatcaataag
     9481 gctgccgttc atacttccgc aataaaaaag gaaggtaaac agcagccatc tttattattg
     9541 tgggaaactt gtgttctaac agcaggagat gcgaaacatg agccaaagga ggacaacact
     9601 gtgtggaaag ctttaagaca gttacttaag aatcagaaaa atcaaagtcc actcgatgaa
     9661 ggttatttac agattccaaa aaaggatata aatctagaat taaaacaaaa gatatttgaa
     9721 ctggagagcc ttggagaagt agtatccaaa aagctgcaaa ataaatatat agatgttttt
     9781 gaagctcagg gtaaaccaaa ccttacaata aaaatttgta gagacataga tggagatgag
     9841 ctaaatgatt taacagctga aagatgtttg gaaaaagaat accgttcaca aatagcaatt
     9901 aatttttatc aacctgcaaa agcctatgat gaagatctag actgcataac aatggatttg
     9961 tggtattact acggtggttt tgtaggaatc ggttgtggaa cattatttga tttaaataat
    10021 aatgacttag aaaaagaaat tgaagaagtg ctaatatttt tactcaacga caaacaatcc
    10081 gagaattaaa aaaagaaagc cccatagggg cttgaattaa tgcatgtatt ttttctcagc
    10141 ttccttaaaa ctcattattt ttagatcaat tcttttctcg tcacttaatt tttcattatt
    10201 atgtgcatca tgagaaggtg gaatgaccac tttaaggcta ctaatatttg ctgtattgtc
    10261 tttaagtaac cagaaagtct gtccttcttt attaaccttt cccaaaaagt ctccgcctac
    10321 attacccatt aatcccattt gcgattctag ctgttcgcct gtatct
//
