LOCUS       NZ_JALAWW010000001     10357 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain T5B6 contig_0000001, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAWW010000001
VERSION     NZ_JALAWW010000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 777518)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 777518)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 332.12328936298655x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 03:03:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,623
            CDSs (total)                      :: 4,534
            Genes (coding)                    :: 4,405
            CDSs (with protein)               :: 4,405
            Genes (RNA)                       :: 89
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 78
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 82 of 129
            Pseudo Genes (incomplete)         :: 79 of 129
            Pseudo Genes (internal stop)      :: 22 of 129
            Pseudo Genes (multiple problems)  :: 45 of 129
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:01:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 523684
            Orig. end                         :: 534041
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAWW010000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10357
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10357
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10357
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5357](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5357)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(33..1493)
                     /gene="spoIVCA"
                     /locus_tag="MOF23_RS02950"
                     /old_locus_tag="MOF23_02940"
     CDS             complement(33..1493)
                     /codon_start=1
                     /gene="spoIVCA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390454.2"
                     /locus_tag="MOF23_RS02950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02940"
                     /product="site-specific DNA recombinase SpoIVCA"
                     /protein_id="WP_223203936.1"
                     /transl_table=11
                     /translation="MKGSSIDSQIEACIKKAGTKDVLKYADEGFSGELLERPALNRLRE
                     DASKGLISQVICYDPDRLSRKLMNQLIIDDELRKRNIPLIFVNGEYANSPEGQLFFAMR
                     GAISEFEKAKIKERTSSGRLQKMKKGMIIKDSKLYGYKFVKEKRTLEIFEEEAKIIRMI
                     FNYFTDHKSPFFGRVNGIALHLTQMGVKTKKGAKVWHRQVVRQILMNSSYKGEHKQYKY
                     DTEGSYVSKQAGNKSIIKIRPEEEQITVAIPAIVPAEQWDYAQELLGQSKRKHLSISPH
                     NYLLSGLVRCGKCGNTMTGKKRKSHGKDYYVYTCRKNYSGAKDRGCGKEMSENKLNRHV
                     WGEIFKFITNPQKYASFKEIEQSNHLSDELELIQKEIEKTKKGRKRLLTLISLSDDDDL
                     DIDEIKAQIIELQKKQNQLTEKCNEIQSKMKVLDDTSSSENALKRAIDYFQSIGADNLT
                     LEDKKTIVNFIVKEVTVVDSDTVYIETY"
     gene            complement(1451..1629)
                     /locus_tag="MOF23_RS02955"
                     /old_locus_tag="MOF23_02945"
                     /pseudo=""
     CDS             complement(1451..1629)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390517.1"
                     /locus_tag="MOF23_RS02955"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02945"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKVIIIEGPQADKCINDCYHYLIKLYRKEIQGDSNICKGIDRGTS
                     DQGIEHRQPDRGLH"
     gene            complement(1885..2064)
                     /locus_tag="MOF23_RS02960"
                     /old_locus_tag="MOF23_02950"
     CDS             complement(1885..2064)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037932.1"
                     /locus_tag="MOF23_RS02960"
                     /note="Built to rescue LMOF2365_14255 during structural
                     annotation change regression.; Built to rescue
                     LMOF2365_14255 during structural annotation change
                     regression; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02950"
                     /product="bacteriocin-like WGxF protein"
                     /protein_id="WP_268314890.1"
                     /transl_table=11
                     /translation="MNHFIFALYTSILLIIAGIVHRIIFRVFNLPFDSTILFWGGFVVI
                     YFILALVTSLLFRK"
     gene            complement(2081..2701)
                     /locus_tag="MOF23_RS02965"
                     /old_locus_tag="MOF23_02955"
     CDS             complement(2081..2701)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010328395.1"
                     /locus_tag="MOF23_RS02965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02955"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_136654241.1"
                     /transl_table=11
                     /translation="MIQLENVYKSFDERSILSNFSIKIRENEFVSIIGKSGSGKTTVLN
                     IMSLLDTPDKGEVQILGCKNPNPKEVMKLRRENLGYIFQNYVLMDNETVLTNLSLSTAY
                     AKEFKKDMLPKALEMVGLDKSFLKKRVYQLSGGEQQRVAIARIILKPCDIIFADEPTGN
                     LDEYNKNIILSLFHQLKDMGKTIVCVTHDQEIARCSERVINLS"
     gene            complement(2698..4884)
                     /locus_tag="MOF23_RS02970"
                     /old_locus_tag="MOF23_02960"
     CDS             complement(2698..4884)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01654.1"
                     /locus_tag="MOF23_RS02970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02960"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_268410275.1"
                     /transl_table=11
                     /translation="MKKMICVLFFLFFISLNIFSYNFIVNKQIAYTLFSNKQVLIINYN
                     KPNFNSEIFLKKLVDFSKKEKVNISQYNFLDEKTLNIYTSNIKDEPSLHIRDWKQPSKE
                     RYLSNFEKISEKDVMGSIKFPSSTWKIKYLDINQVNNVGIGNRFYISTTDNIITEKAIR
                     QFSNFGDVSLESNNTINSYMFNTTLALLVLLSIIILFIGILYFAIKNRKQLILHKLWGY
                     SKIKSLFFLPNLFLKPFVIIILFVALLIVILTFTFNLNDWIIIYAKLYIINIIIAILLL
                     TGYKIMITTVLYNNSDISGATKGSMPFKKFQMLSLGFKLVITVLLFNVIALSFTNLFDL
                     KQQLKNKSYWNKTQTIYKLNVTDTGIDYSNLKNDREKNDKLNKLYHELESHKNAFIMYA
                     DNYSVLERKGGKPIYFYTLNSTKENEVYSPSGRNVTISPNYLKINPIIGIKGKQITKKQ
                     LNLDPNTLNLLVPKKYAKFKEKIIKAYKEQFYFQKVEVENMYNKEIGHTSNALKKDRLK
                     INIIYTENSQKYFTFNSELGDINKKNNIVDPIATIFTGNVDTSYIGAYATTSLFFYDDS
                     EGMAYQKLVPYLEKTHTKNLINASVSVYKELSEQIARIQNMFIQNLIGSVIAIILSMAF
                     LVSYIWTYYSVNAYNLYLKEIFGYSYWKRNKHLIIFSLTSNFLLGAGVSLYYNLNLLII
                     FIFLFIGLELIVLYIMSRYLNRKNMNKILKGDRI"
     gene            complement(5001..5357)
                     /locus_tag="MOF23_RS02975"
                     /old_locus_tag="MOF23_02965"
     CDS             complement(5001..5357)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01653.1"
                     /locus_tag="MOF23_RS02975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02965"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_169051586.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 4.3e-09,
                     bitscore: 32.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKVFVTFALGVGLLVPFAAFASNSDEITHGEVDLDKTRNSMTI
                     FKTEEVGGGTWKHGFSKQDNVYSHYNHKKKTHKASVSVGQTLTEGPWKKKNEGYSKASR
                     PAGLFGNKAYWNTK"
     misc_feature    complement(5319..5334)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 21.07"
                     /tool="antismash"
     gene            complement(5695..5856)
                     /locus_tag="MOF23_RS02980"
                     /old_locus_tag="MOF23_02970"
     CDS             complement(5695..5856)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="MOF23_RS02980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="MOF23_02970"
                     /product="hypothetical protein"
                     /protein_id="WP_268410277.1"
                     /transl_table=11
                     /translation="MTIKKKFVLSLILLGLIGGGITSVSSFQSKDLAIDRNTTFLHIHH
                     KDNQKYFA"
     gene            complement(5846..6976)
                     /locus_tag="MOF23_RS02985"
                     /old_locus_tag="MOF23_02975"
     CDS             complement(5846..6976)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014479091.1"
                     /locus_tag="MOF23_RS02985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02975"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_268410279.1"
                     /transl_table=11
                     /translation="MNPVLPSSHVGVKITEWYKMIRQFSIPDAEILKAEVTQEIERMEE
                     DQDLLIYFQLMCFRHQLMLDYLEPENKYGDRPTIKELLEKIEAPQKKLTGLLKYYSLFF
                     RGMYEFDQKEYVEAINYYRKAEKELGYVTDEIEKAEFHFKVAEAYYIMKQTHVSMHHIL
                     QALAIYDTHTLYQIRKIQSLFVIAGNYDDFKCYDKAIPHLKNALQIADKIYNKRMMIIA
                     NLNIGLSYDRAGNYIEAALYFKEALRLIGEKESDLFTKVYFGLCCALFKSGHIMDGVPV
                     LEKGIEYLDKNPNELYRQLFDFLDALYVQGIYETKILEVLEYLDSKKLYAYIEECVKCV
                     AVEYEKANDHLKASRYYKAVLETQTKIQRGDCLYDY"
     misc_feature    6943..6957
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.6"
                     /tool="antismash"
     misc_feature    complement(7002..7018)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.62"
                     /tool="antismash"
     gene            7155..7292
                     /gene="phrE"
                     /locus_tag="MOF23_RS02990"
                     /old_locus_tag="MOF23_02980"
     CDS             7155..7292
                     /codon_start=1
                     /gene="phrE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390461.1"
                     /locus_tag="MOF23_RS02990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02980"
                     /product="phosphatase RapE inhibitor PhrE"
                     /protein_id="WP_134975483.1"
                     /transl_table=11
                     /translation="MSKSKLFISLSAVLIGLAFFGSMSNGEMKEASRNVTLAPTHEFLV
                     "
     gene            7515..9110
                     /gene="yqcG"
                     /gene_synonym="rttG"
                     /locus_tag="MOF23_RS02995"
                     /old_locus_tag="MOF23_02985"
     CDS             7515..9110
                     /codon_start=1
                     /gene="yqcG"
                     /gene_synonym="rttG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390463.1"
                     /locus_tag="MOF23_RS02995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02985"
                     /product="LXG family T7SS effector endonuclease toxin YqcG"
                     /protein_id="WP_268410281.1"
                     /transl_table=11
                     /translation="MKVFEAKTLLSEAEKRAKEYKDLKSKMVKLKKAFKAVADLDDSEF
                     SGKGANNIKSFYEDQAGIADQWIDLIEMKISFLTSIPGFLEDANLSDAYIEETFLAHEL
                     ANAYTKSKSIMSEQKKAMKDILNDINDILPLDLFSTETFKNELSSAEKKRKEAIEKMDE
                     VDENLTSEYGLSEANEQMIQADYQALMNATAKGKSASPIHYNAMAYRDSEIHKMTEDVK
                     KQSTDYISFKDQQAEQRRIAKEQEELANRPWYEKSWDAVCNFTGEVSGYYDYKRAADGV
                     DPVTGEKLTAGQRVAAGAMAAAGYIPIVGWAGKLAKGGKAVYSTSKALYRADKALDVYK
                     TPKTFHALHNSSKGLYGLASANGFSEAITGRDMFGNKVSKERQEQSLSGAMAMLVPFGA
                     RGINKKLNAKSSSRVSETSTNTSKKPKVPKTYKRPTYFRKGVRDKVWENAKDSTGSVKD
                     PLTKQVMKKDEPWDMGHKPGYEFRKHQQSAMERNISRKQFLDEHNNPDHYQPELPSSNR
                     SHKGEDMTDDYFGD"
     gene            9125..9703
                     /gene="yqcF"
                     /locus_tag="MOF23_RS03000"
                     /old_locus_tag="MOF23_02990"
     CDS             9125..9703
                     /codon_start=1
                     /gene="yqcF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_390464.1"
                     /locus_tag="MOF23_RS03000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOF23_02990"
                     /product="type VII secretion system immunity protein YqcF"
                     /protein_id="WP_015714310.1"
                     /transl_table=11
                     /translation="MGVTQENKVIARTVLGAFGGKPKVTKYWDDNKSSSIDILSVSDQP
                     QEGITSYSTLGLSDHSINYEVNGTPLRIEIVAAMESASDIYANVLSTCAFNIINSNFTC
                     APGVIFKNVISMYDQETDMKHIMFVPPFLWEEDLELLEFSNKNVTWLMALPISEGELQV
                     AEKHGSDYLQDLLESKQIDIFDIKRESVV"
ORIGIN
        1 taattacaaa aggggggtgc atacacccct ctttaatacg tttcaatata tacggtgtca
       61 gaatccacaa cggtaacttc tttcacgata aagttaacaa ttgttttttt atcttcaaga
      121 gttaagttat ctgcaccgat tgattgaaaa tagtcgatgg ctctttttag agcattttcg
      181 cttgagcttg tatcatctaa tactttcatt tttgattgaa tttcgttaca cttttcagta
      241 agctggtttt gctttttttg cagctcaata atttgtgctt tgatttcatc aatatctaaa
      301 tcatcgtcat cgcttaggct gattagcgtt aaaagacgct tgcggccttt ttttgttttc
      361 tctatctctt tttgaataag ttctaattca tcagacaggt gatttgattg ttcaatctct
      421 ttaaaagaag catacttttg gggatttgtg atgaatttaa aaatttcacc ccatacatgt
      481 cggtttaatt tattctcaga catttctttt ccgcagccgc ggtcctttgc tccagaataa
      541 tttttccggc aagtatacac atagtagtct ttaccatgtg attttctttt cttccctgtc
      601 atggtatttc cgcatttccc gcatctaacc aaacccgata acaagtaatt gtgagggcta
      661 atactcaagt gttttctttt actttgacct aagagttctt gagcataatc ccattgttca
      721 gctggaacaa ttgctggaat tgccacagtg atttgttctt cttcaggcct tattttaatt
      781 atagatttgt tccctgcctg ctttgaaaca taggaaccct ctgtatcata tttatattgt
      841 ttatgttcac ccttataaga agagttcatt aatatctgtc gaacaacctg cctatgccat
      901 actttagctc ctttttttgt tttaaccccc atctgagtta aatgtagagc aatcccattt
      961 actctgccga aaaaagggct tttatgatcg gtgaaatagt taaaaatcat ccgaatgatt
     1021 tttgcttcct cttcaaatat ctcaagagtt cttttctctt taacaaattt atagccatat
     1081 agtttagaat ctttaatgat catgcctttt ttcatttttt gaagtcggcc gcttgatgtc
     1141 cgttctttga ttttggcttt ttcaaattct gagattgctc cacgcattgc gaaaaataat
     1201 tgaccttctg gagaattggc gtattctcca tttacaaata tcaatggtat atttcgcttt
     1261 cggagttcat catcaatgat aagctgattc attaatttcc gagaaaggcg gtccggatcg
     1321 taacaaatga cttgacttat aagccccttg ctggcatcct ccctcaagcg atttaaagcc
     1381 ggacgttcta aaagctctcc agaaaatcct tcatcggcat acttcagcac atccttagtc
     1441 cctgctttct ttatgcaggc ctcgatctgg ctgtcgatgc tcgatccctt gatcgcttgt
     1501 tcctcggtcg atacccttac atatattgct atcaccctgg atctccttcc tataaagttt
     1561 tattaaataa tgatagcaat cgttaatgca tttgtcagct tgcggtcctt cgatgattat
     1621 taccttcata aaaatgcatc accccttggg taatgctatg taaaaggtgt ttgtcctaat
     1681 gagtttattt tataatgtgt atatgggttg taaagaaaga gaaaaaagac cattgtatta
     1741 aacaatggtg ttttacagga gaaacacaaa ctcaacagcc tcatagaaac aagcggtttg
     1801 tgtatatata caaaagctag agttcaagag tctcatgcct tagttatata atctaggtct
     1861 tgcaaatgtt ctgagattac tatgttattt cctgaataat aaggaagtca ctaaggctaa
     1921 tataaagtaa ataactacaa atcctcccca gaacaaaatt gtactatcaa acggtagatt
     1981 aaacactcta aatattattc tatgaacgat acctgcaata attaaaagga tgctagtata
     2041 cagtgcaaaa ataaaatgat tcattttgtt tcaactcccc ttatgaaaga ttaattactc
     2101 tttcagaaca tctagctatt tcttgatcat gcgttacaca tacgatagtt ttccccatgt
     2161 ctttcaactg atgaaataaa gataaaataa tgttcttatt atattcatct aaatttcctg
     2221 ttggttcatc agcaaaaata atatcacaag gtttcaaaat tattctagct atagcgacgc
     2281 gttgttgctc tccaccactt aactgataga ctcttttctt taaaaagctt ttatctaaac
     2341 cgaccatctc taaagctttt ggtaacatat cttttttaaa ttctttggca taagcagtgg
     2401 aaagtgacag gtttgttaac actgtctcat tgtccataag cacatagttt tgaaaaatgt
     2461 aacctaagtt ctctctcctt aatttcatga cttcttttgg gttaggattt ttacatccta
     2521 atatttggac ttctccctta tccggagtat ccaataagct cattatattt aaaacagtgg
     2581 tttttcctga accactcttt ccaataatag aaacaaattc attttctctt attttaatgg
     2641 aaaaattaga aagaattgat ctttcatcaa aacttttata tacgttttca agttggatca
     2701 tattctatcc ccttttaata ttttattcat gttttttcta ttcaaatatc tactcataat
     2761 atatagaaca attaattcta atcctataaa caaaaaaatg aaaataatta ataaatttaa
     2821 attgtaataa agacttactc ctgctcctag caaaaaatta gaagtcagtg aaaaaattat
     2881 taaatgctta tttcttttcc aatatgaata tccaaatatc tctttcaaat atagattata
     2941 agcattcaca ctatagtaag tccatatata tgaaactaaa aaggccattg ataaaataat
     3001 tgctatcaca gaaccaatta aattttgaat aaacatattc tgaattcttg caatttgttc
     3061 acttaattct ttgtatacag atactgacgc gttaatcaag ttttttgtat gcgttttctc
     3121 taaatatgga actaattttt gataagccat accttcagaa tcatcataaa aaaataatga
     3181 tgtagttgca tatgctccta tgtaagaagt gtcaacatta ccagtgaata tagtggcaat
     3241 aggatcaaca atgttgtttt ttttatttat atcaccaagt tcgctattaa aggtgaaata
     3301 cttttgacta ttttcagtat atattatatt tattttcaaa cggtcttttt tgagagcatt
     3361 tgacgtatgg cctatttctt tgttatacat attctccact tcaacttttt gaaaataaaa
     3421 ctgttcctta taagctttga tgattttttc tttaaacttt gcatattttt taggaacaag
     3481 cagattcaac gtatttgggt caaggtttaa ttgttttttt gtgatctgtt ttccttttat
     3541 gccgataatc gggtttattt tcaaataatt aggacttatt gttacatttc ttccagaggg
     3601 agaatatacc tcattttctt tagtcgaatt gagtgtataa aaatatatcg gctttccccc
     3661 tttcctttct aatactgaat aattgtcagc atacattata aatgcatttt tgtgagattc
     3721 aagctcatga tagagcttat tcagtttatc atttttttct ctatcgtttt taagattact
     3781 atagtcaatt cctgtatcag taacgttgag tttgtaaatt gtctgagtct tattccagta
     3841 tgatttattc tttaattgtt gttttaaatc aaataagttc gtgaatgata gggcaataac
     3901 gttaaataat aaaactgtta tgactaactt aaatcctagt gataacattt gaaatttttt
     3961 aaatggcata ctacctttag tagcgccact tatatcactg ttattatata aaacagtggt
     4021 tatcattatt ttgtaccccg ttaataataa aatcgcaatt ataatattta ttatatacag
     4081 tttagcatat atgataatcc aatcattaag attaaaagta aatgtaagta taacaattaa
     4141 aagggcaaca aataatatta taataacgaa cggttttaga aataaatttg gtaagaagaa
     4201 aagcgatttg atttttgaat atccccataa tttatgtaaa attagttgtt ttcgattttt
     4261 aattgcaaaa taaaggatac caataaataa gattataata gaaagcaaaa ctaacaaagc
     4321 taatgtagta ttaaacatat acgaattaat agtgttatta ctttcaagac tcacatcacc
     4381 aaagtttgaa aactgacgaa ttgccttttc tgttattata ttgtcagtag ttgaaatgta
     4441 aaaccgattc ccaattccca cgttatttac ttgatttata tcaagatact ttattttcca
     4501 tgttgatgat gggaatttaa ttgagcccat tacatctttt tcgcttattt tttcaaaatt
     4561 agataaatat cgttccttcg atggctgttt ccaatcccta atatgtaagg aaggctcatc
     4621 ttttatattc gaagtataaa tatttaatgt tttttcatcc aaaaaattat actgcgaaat
     4681 attaactttt tctttcttag aaaaatcaac aagcttcttc aaaaatatct cactattaaa
     4741 gttaggttta ttataattaa taattaaaac ttgtttatta gagaacaaag tatacgctat
     4801 ttgtttattg acaataaagt tataactgaa tatattcagt gagataaaaa acagaaagaa
     4861 taatacacag atcattttct tcatatgtaa acctccataa aaaatccagt gtctcttcta
     4921 taggagttaa tagaatacac gtttcgaatt tatagacacc ggattcaaga gaaacttata
     4981 ttatatttat ggaatcgctt ttattttgta ttccaataag ctttgtttcc aaatagcccc
     5041 gcaggtctag atgcttttga gtaaccctca ttttttttct tccaaggtcc ctcagttaat
     5101 gtttgtccta ctgaaacact agctttgtga gtcttctttt tgtgattata atgagaataa
     5161 acattatcct gtttagaaaa cccatgcttc caagtcccac cacccacttc ttcagtttta
     5221 aagatagtca tagaatttct tgttttatcc aagtctacct ccccatgagt aatttcatca
     5281 gagttgctag caaatgctgc aaatggaacc agtaaaccta cccccaacgc gaatgtaaca
     5341 aaaacctttt tcttcaacgt aatctctcct ttgaaaaaat tttataaggg ttatgtaaaa
     5401 tataccctct atatcaggat tatagcatat atgtataatt ttgtatatat acttatgaca
     5461 ttatacactt tctttaaaac ttttaaggag attttgaacc agccaatttc atctcgatag
     5521 ggctatttaa cttccgttcc acctttaatt actgctatgt tatggcataa tattaactct
     5581 ttagttttca aaccataact ttgtattgta cggaggtttt aatattgctc ttcaggtatt
     5641 tcagcagcga gactcaaacc tctcaaaacc aaaaaagccc ttttcaaggg caaattatgc
     5701 aaaatatttt tgattatctt tatgatggat atgcaggaat gtagtgtttc gatcgatcgc
     5761 tagatcttta gactgaaaac ttgagacact agtaattcca ccacctatta accctaataa
     5821 aattagagaa agaacgaatt tcttcttaat agtcataaag acaatctcct ctctgaattt
     5881 tagtttgcgt ttctaaaact gctttatagt acctggatgc tttcaagtga tcatttgctt
     5941 tttcatactc aacagccacg cacttgacac attcttcaat gtaagcatat agttttttag
     6001 agtccaggta ctctaagacc tcgagaattt ttgtttcgta aataccttga acatacaaag
     6061 catccaagaa atcaaataat tgacgataca actcgtttgg atttttatca aggtactcta
     6121 tgcctttttc taatacaggt actccatcca ttatatgacc agatttaaac aaggcacaac
     6181 ataatccgaa gtaaactttt gtgaacaaat cactttcttt ttctccgatt aatctaagtg
     6241 cttctttaaa atataaagca gcctctatat aatttcctgc cctatcataa ctaaggccaa
     6301 tatttaggtt agcaataatc atcatgcgct tattataaat tttgtcagct atttgaaggg
     6361 cattttttaa atgaggaata gccttgtcgt aacatttgaa atcatcgtaa ttaccggcta
     6421 taacaaacag gctctgaatt ttccgaattt ggtaaagagt atgtgtgtca taaatagcta
     6481 gagcttgcaa aatatgatgc attgatacat gagtttgttt cattatgtaa taagcttcag
     6541 ccactttaaa gtgaaattct gctttttcaa tttcatcagt gacatacccc agctcttttt
     6601 cagctttacg atagtaattg attgcctcaa catactcttt ttgatcaaat tcatacatac
     6661 cgcgaaaaaa caaggaataa tacttcaaaa ggcctgtaag cttcttctga ggagcttcaa
     6721 ttttctcaag aagttctttt attgttggtc gatccccata cttgttctct ggctctaaat
     6781 aatcaagcat aagttgatgc cgaaaacaca ttaattgaaa atatataagt aaatcttgat
     6841 cctcttccat acgctcaatt tcctgtgtca cttctgcttt taaaatttca gcatccggaa
     6901 tactaaactg acgtatcatc ttataccatt cggtaatctt aacccctaca tgtgaggaag
     6961 gtaaaaccgg attcacatga accctccttt tatgtctttt tatgtaatat tctaacattt
     7021 ctaaaaagtg tttggaccgc aaaaaaaccg caaacttttt aacgttttca tcataaaagg
     7081 tgcaacatga tatgcgcagc ccaaacagca gcaggactcc ccttccaata aataggacat
     7141 agactaaaaa gcaaatatcg aaatctaaat tgtttatcag tttatccgcc gttttaattg
     7201 gacttgcctt tttcggatct atgtctaatg gcgaaatgaa ggaagcatcc cggaatgtaa
     7261 ctctagcacc tactcatgaa ttccttgttt gattcgataa acaacattag ttctaattcc
     7321 ctttagccgt tgaataacgg ctttttcttt ttaaacccta agaatctttc cactaaagct
     7381 gtctcaattc taaaaataga tcaaaagaca cagatcgaat gattcatttc caccatttga
     7441 tccacataac agttagtttc atcattcttc catggtaata ttttgttatg acgattaaat
     7501 gaaaggatat gatcatgaaa gtatttgaag ccaaaacctt gctctccgag gctgaaaagc
     7561 gagcaaagga atacaaagat ttaaaaagta aaatggtcaa attaaagaaa gcgttcaaag
     7621 ctgttgcaga tttagatgat agcgagtttt caggcaaagg tgctaataac atcaaatcat
     7681 tttatgaaga tcaagctggc attgctgacc aatggattga tttaattgag atgaaaattt
     7741 ctttcttaac gagtattcct ggtttcttag aagatgccaa cttatcagat gcctacatag
     7801 aagaaacctt tttagcgcat gagcttgcta acgcctacac caaatcaaaa tccattatgt
     7861 ctgaacagaa aaaagccatg aaagatatat taaatgatat caacgatata ctgcctttgg
     7921 atctgttctc gacagaaact tttaaaaatg aactttcgtc tgccgaaaag aaacgaaaag
     7981 aagccattga gaaaatggat gaagttgacg aaaacttaac atcagaatac ggactatcag
     8041 aagcaaatga acaaatgatt caagctgatt atcaagcttt aatgaatgcg acagcaaaag
     8101 gcaaaagcgc ctctcctatt cactataatg cgatggctta tagagacagt gagattcaca
     8161 agatgactga ggatgtaaag aaacaatcaa cagactacat ctcttttaag gaccaacagg
     8221 cggaacaaag aagaatagca aaagaacaag aagaactggc gaacagacca tggtatgaaa
     8281 agtcttggga tgctgtttgt aattttacag gagaggtctc tggatattat gattataaaa
     8341 gagccgctga tggggttgat cctgttaccg gcgaaaagct aactgctgga caacgggtcg
     8401 ctgccggtgc aatggctgcg gcaggataca tccctatcgt aggatgggca ggtaaattag
     8461 ctaaaggcgg aaaagctgtt tattcaacaa gtaaggcatt gtatagagca gacaaagctc
     8521 tcgatgtata caaaacacct aagacattcc atgctcttca caactccagc aaaggacttt
     8581 atggacttgc ttctgcaaat ggttttagcg aggccataac aggacgcgat atgttcggga
     8641 ataaagtctc taaagagagg caagaacaga gccttagtgg agcaatggca atgttggttc
     8701 ctttcggagc tcgtggaatt aacaaaaagc ttaatgcaaa atcgagttcc cgagttagcg
     8761 aaacatctac taacaccagc aaaaaaccta aagttcctaa aacgtacaaa agaccaactt
     8821 atttccgtaa aggcgttaga gataaggttt gggaaaatgc taaggattca accggatctg
     8881 ttaaagaccc gttaacaaaa caggtaatga aaaaagatga accatgggat atgggtcata
     8941 aaccaggtta tgaatttaga aaacatcaac aaagtgcaat ggaaagaaat attagtcgaa
     9001 agcaattttt agatgaacat aataatccag accattatca acctgaatta ccttcctcca
     9061 ataggagtca taaaggggaa gatatgacgg atgattactt tggagattaa ggagagattg
     9121 tctaatgggc gttacacaag aaaataaagt aattgctaga acagttttag gagcttttgg
     9181 aggtaaaccg aaggttacga aatattggga tgataacaag agtagcagca ttgatatttt
     9241 atcggtcagt gaccagcctc aggaaggtat cacgtcatat tcaactctag gtttgtcgga
     9301 tcattctatt aattatgagg tgaatggcac tccgttgcgg atagaaatcg tagctgcaat
     9361 ggagtcagct tcagacatat atgctaatgt tttatcaact tgtgccttta atataatcaa
     9421 ctcaaatttc acttgtgcac caggtgttat ttttaaaaat gtgattagta tgtatgatca
     9481 ggaaaccgat atgaaacata ttatgttcgt tcccccattt ttatgggagg aagatttaga
     9541 actacttgaa tttagtaaca aaaatgttac ttggctaatg gctttaccta tatccgaagg
     9601 agaattgcaa gtagctgaaa aacacggctc agattattta caagatcttc tggaatcaaa
     9661 acagattgat atttttgata taaagagaga atctgttgtt taatgacttt aataaagaag
     9721 agtcacttta taagcccctc tcaatgagaa gggcttattc atatgataca agataataga
     9781 acaaagtcat tttttgaata gtttatataa ccagataccg aagttagaaa atgtacgcat
     9841 cgaagggatc tacataaaag tagaaggccg aaaagcaaca ttgcattttg acatgccatt
     9901 ttacgctgaa aacattccga agaatgggct ggttcaaggt acaactcaat tgcccttcaa
     9961 gtggactttt tcaaaactta ttcattagaa atgaatactg tatcagatac atacagagga
    10021 aacattaaca taaataaaga tgaaaaaggc ttattagaca ttaatggaca ggagaaatta
    10081 cagcaataat taaagcagaa tacaggatta ttcaatcaat aaatggctat atttgcggag
    10141 ttatcaaata gactatcacc tttttagcta tatcggagca gattaatgag ggctctattg
    10201 cttttataag gaatttcgga caggaggagg caaactcctg tcccgttttt taaaacacag
    10261 tgagagaaaa tcagtgacat tactatcctt attgacctga cacaaatgat gaccgtggac
    10321 cttcaagaca tgcatcaata cgtgcaactt gttcttg
//
