LOCUS       NZ_JALAWB010000022      6849 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain EC30O4N-B4-2 contig_0000022, whole
            genome shotgun sequence.
ACCESSION   NZ_JALAWB010000022
VERSION     NZ_JALAWB010000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 324.93002258901475x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:42:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,266
            CDSs (total)                      :: 4,182
            Genes (coding)                    :: 4,055
            CDSs (with protein)               :: 4,055
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 75
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 73 of 127
            Pseudo Genes (incomplete)         :: 74 of 127
            Pseudo Genes (internal stop)      :: 20 of 127
            Pseudo Genes (multiple problems)  :: 33 of 127
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:21:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6849
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAWB010000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6849
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="EC30O4N-B4-2"
                     /submitter_seqid="contig_0000022"
     region          1..6849
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..6849
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6849
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[5256:>6849](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5257..>6849
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            complement(39..584)
                     /gene="iseA"
                     /locus_tag="MOE87_RS20795"
                     /old_locus_tag="MOE87_20750"
     CDS             complement(39..584)
                     /codon_start=1
                     /gene="iseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389720.2"
                     /locus_tag="MOE87_RS20795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE87_20750"
                     /product="DL-endopeptidase inhibitor IseA"
                     /protein_id="WP_003241937.1"
                     /transl_table=11
                     /translation="MKKCFLFLTTIALILSLSTNAFAKNTSGDLSQKQALQLALSAREH
                     FWNTMSGHNPKAKKAVCPSGTFEYQNLQYVYMCSDLGTKAKAVNYLTPIFTKTAIEKGF
                     KDYHFTVSKGKLAVPIGDGDNLLNWKKSTAKLISKKGGTVTYEFTVPTLDGSPAAKRKV
                     TFVKENKKWKVNQFDAVI"
     gene            872..2263
                     /locus_tag="MOE87_RS20800"
                     /old_locus_tag="MOE87_20755"
     CDS             872..2263
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389719.2"
                     /locus_tag="MOE87_RS20800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE87_20755"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_003241939.1"
                     /transl_table=11
                     /translation="METSQEQLVTQKKSNPSVWRSMSLFLVPLLLSNVLQSVGQLVGMM
                     AVGRWLGVDAVAAVSSFFPLFFLLISFTIGIGSGSSILIGQAYGAKNEERLKAVVGTTL
                     TFTFLLGVVLAVIGSIFTLDILRLMGTPENVIHVSASYARILFYAMPFMFLYFAYTTFL
                     RGTGDSKTPFYTLIVSTVINIALLPVLILGMFGFPKLGIYGSAYATVISTIATFIVLMV
                     YLRKRNHPLQFDKTVRRYLKMDKELLALLLRLGIPSSINMILVSLSEIAVISFVNHYGS
                     NATAAYGVVNQVASYVQMPAVSLGIAVSIFAAQSIGANEFGRLKQVIRVGIWLNYIIGG
                     VLIILIYVFSHQILGLFLTEQETLYIAHRLLMITLWSYLLFGNAQIISATMRASGTVLW
                     PTVISIFAIWGVEVPVAFVLSHYTNLEILGVWVGYPAAFAVSLLLIYGYYQFVWKKKQI
                     TRLIQ"
     gene            2396..3373
                     /gene="galM"
                     /locus_tag="MOE87_RS20805"
                     /old_locus_tag="MOE87_20760"
     CDS             2396..3373
                     /codon_start=1
                     /gene="galM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389718.1"
                     /locus_tag="MOE87_RS20805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE87_20760"
                     /product="aldose 1-epimerase"
                     /protein_id="WP_225516923.1"
                     /transl_table=11
                     /translation="MANDIEKITYLGTPAIKAGNEHIEMIVVPEWGSNVISLVDKTTQL
                     QLLREPETAESFHDTPTLYGIPILFPPNRISDGTFSFRGRTYHFDINEKDKHNHLHGFL
                     YQEKWNVVTMKQTDEEVIVETEIDLSELPHVYKQFPHHAVVRMTYTLKGNTLFKHATVM
                     NKGKEAFPWGIGYHTTFVFPEESSLFSLTADQQWELDERLLPTGKLMDVQNKEALHEGM
                     DLRSQQLDDVFLSSYQKRGGENQAVIYHQHAHISIIYKADEQFKHWVVYNADGKQGYLC
                     PEPYTWVTNAVNLNVSPSLTGVQVLEPGEETTAKSSITIELNHQ"
     gene            3408..4886
                     /gene="dacB"
                     /locus_tag="MOE87_RS20810"
                     /old_locus_tag="MOE87_20765"
     CDS             3408..4886
                     /EC_number="3.4.16.4"
                     /GO_function="GO:0004175 - endopeptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="dacB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241941.1"
                     /locus_tag="MOE87_RS20810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE87_20765"
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="WP_268427572.1"
                     /transl_table=11
                     /translation="MKKSIKLYVAVLLLFVVASVPYMHQAAQAAEKQGALAGQIDKILA
                     DHPALEGAMAGITVRSAETGAVLYEHSGDIRMRPASSLKLLTAAASLSILGEDYSFTTE
                     VRTDGKFKGKKLNGNLYLIGKGDPTLLPSDFEEMAEKLKQSGVKEIKGNLIGDDTWYDD
                     IRLSPDMPWSDEYTYYGAQISALTASPNEDYDAGTVIVEITPGKKEGEKPAVSVSPKTD
                     YVTIKNDAETTAAGQKKDLTIEREHGTNTITIEGSVPVDASKTKEWIAVWEPAGYALDL
                     FKQALKKQGITVKGHVKTGAAPGSSDVLISHRSMPLSKLFVPFMKLSNNGHAEVLVKEM
                     GKVKKEEGSWEKGLEVLNNALPEFGVDPKSLVLRDGSGVSHINAVSSDQISQLLYDIQD
                     EKWFSAYLNSLPVAGNSDRMIGGTLRNRMKDTPAQGKVRAKTGSLSTVSSLSGYAETKS
                     GKTLVFSILLNGLIDDEDGKDIEDQIAVILANQS"
     misc_feature    complement(5044..5057)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.77"
                     /tool="antismash"
     gene            5257..>6849
                     /locus_tag="MOE87_RS20815"
                     /old_locus_tag="MOE87_20770"
     CDS             5257..>6849
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_Starter (3-300). E-value:
                     1e-89. Score: 292.8. Matches aSDomain:
                     nrpspksdomains_MOE87_RS20815_Condensation_Starter.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOE87_RS20815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE87_20770"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268427574.1"
                     /sec_met_domain="Condensation (E-value: 6.5e-76, bitscore:
                     243.6, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2e-13, bitscore:
                     38.0, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQHTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVL
                     EGALNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQA
                     RIPFKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDP
                     LPDQPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSAS
                     RDTITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKA
                     EKEMLGMFVSSLPIRITVDPDADFLSFVRRVGREQLSVMRHQRFPYNLLVNELRKEQKD
                     LHNLIGISMQYQPLQWHNADGFDYETALYFSGYTATELSVQIQERTDTGTIQLNFDYQN
                     TLFSLEDIKRIQNHLLTLLENALQHPHSFIKELDMTNIREKQKLLYEFNKTEAVSPEAP
                     TLHGLFERQAAVTPERPAIRFSSDSLTYAELDMYASRLAARLAARGVTKESIVGVLSER
                     SPEMLIAVLAVLKA"
     aSDomain        5266..6156
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE87_RS20815_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="1.00E-89"
                     /label="MOE87_RS20815_Condensation_Starter.1"
                     /locus_tag="MOE87_RS20815"
                     /protein_end="300"
                     /protein_start="3"
                     /score="292.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIMDG"
                     /tool="antismash"
                     /translation="HTYSLTHAQRRVWFTELLEPGTSICNLTACVKFRGNIDLDVLEGA
                     LNHSISRNDAIRFQLLEGEELEPRLYLTEYKYTSLGIIDFSNVEMIEIEQWIQEQARIP
                     FKLFNSPLYQFYLLRIHNHEVWLFAKFHHIIMDGISLNVMGNQIIDLYQKMKKQDPLPD
                     QPEPSYLSYIEKESQYLQSSRFTKDRLFWTQTFQNPPGYHSLAGQTSLQKQSTSASRDT
                     ITLSPHLGQTIRIFCEEQKINIISLFMASFYICISRITSKKDLAIGTYYGNRGSKAEKE
                     MLGMFVSSLPIRITVD"
     CDS_motif       5272..5310
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE87_RS20815_0001"
                     /evalue="2.80E-03"
                     /label="C1_starter_004-017"
                     /locus_tag="MOE87_RS20815"
                     /protein_end="18"
                     /protein_start="5"
                     /score="10.5"
                     /tool="antismash"
                     /translation="YSLTHAQRRVWFT"
     CDS_motif       5335..5451
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE87_RS20815_0002"
                     /evalue="3.30E-10"
                     /label="C2_starter_024-063"
                     /locus_tag="MOE87_RS20815"
                     /protein_end="65"
                     /protein_start="26"
                     /score="32.6"
                     /tool="antismash"
                     /translation="ICNLTACVKFRGNIDLDVLEGALNHSISRNDAIRFQLLE"
     CDS_motif       5656..5721
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE87_RS20815_0003"
                     /evalue="5.00E-08"
                     /label="C3_starter_134-155"
                     /locus_tag="MOE87_RS20815"
                     /protein_end="155"
                     /protein_start="133"
                     /score="25.8"
                     /tool="antismash"
                     /translation="FHHIIMDGISLNVMGNQIIDLY"
     CDS_motif       6055..6144
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE87_RS20815_0004"
                     /evalue="6.30E-09"
                     /label="C5_starter_265-294"
                     /locus_tag="MOE87_RS20815"
                     /protein_end="296"
                     /protein_start="266"
                     /score="28.3"
                     /tool="antismash"
                     /translation="DLAIGTYYGNRGSKAEKEMLGMFVSSLPIR"
     CDS_motif       6196..6309
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE87_RS20815_0005"
                     /evalue="9.50E-10"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="MOE87_RS20815"
                     /protein_end="351"
                     /protein_start="313"
                     /score="31.0"
                     /tool="antismash"
                     /translation="REQLSVMRHQRFPYNLLVNELRKEQKDLHNLIGISMQY"
ORIGIN
        1 atcttatggg gtcagtccct tatgcagggc gttttttctt atataactgc gtcaaattga
       61 ttgactttcc attttttgtt ttcttttaca aatgtcactt tccgtttcgc tgcaggtgat
      121 ccgtctaaag taggaacggt aaattcgtat gtgactgtgc cgcctttttt agagatcagt
      181 ttagcagttg attttttcca atttaaaagg ttgtcaccgt cgccgattgg cacggctagt
      241 tttccttttg aaacagtgaa gtgatagtct ttaaagcctt tttcgattgc tgtttttgtg
      301 aaaataggag ttaagtaatt cactgctttt gctttagtgc ctaggtcact gcacatgtac
      361 acgtattgaa gattttgata ctcaaaagtg cctgatgggc aaactgcttt tttcgctttt
      421 ggattgtggc cgctcattgt gttccaaaaa tgctctcttg ctgataacgc cagctgcagt
      481 gcttgttttt gtgataaatc acctgaagta tttttcgcaa atgcatttgt gcttaatgac
      541 agaataagtg caatcgttgt tagaaataga aaacattttt tcattgtggt tcactccttc
      601 aaaatctctt tctcttatgt tacaaatgtt acaataatta cggatagatt acaatacttt
      661 tgcataaatt attttttatt ctttttactc acaagaaaaa aggctgttac ttgctagttt
      721 attcttcata agctaaaaag atgtgtaaaa ttagaagaat cgggaagggt ttcatatgta
      781 cagtgatgaa taataacatg ttacatatcc tgtttttgtc aaaagaatag ggtcatgttt
      841 acaaacgaat tcaaataaag gagctttcaa tatggaaaca tcacaagaac aacttgtaac
      901 tcagaagaaa agcaatccat ctgtctggcg gtctatgtca ctctttttag ttccgcttct
      961 gctaagcaat gtactgcaat cagtcggcca gttagtcggg atgatggctg taggcagatg
     1021 gcttggtgtt gatgcggttg cagctgtatc atcctttttc ccgttatttt ttcttttaat
     1081 ctcatttaca attgggatcg gttcaggaag ctcgatcctg atcggacaag cctacggggc
     1141 gaaaaatgag gagcggttaa aggctgttgt cgggacgacg ctcacgttta cgtttttatt
     1201 aggggttgtg ttagccgtca tcggcagtat tttcaccttg gatattcttc gtttaatggg
     1261 cacgcctgaa aatgtaattc atgtcagcgc cagctatgca cgtattttat tttacgcgat
     1321 gccgtttatg tttttatatt ttgcttatac aacatttttg cgcggaactg gggattctaa
     1381 gacacctttt tatacgctga ttgtgagcac ggtcattaac atagcgcttt tgcctgtctt
     1441 gatccttggc atgttcggtt ttccgaaact gggcatatac ggctccgcat atgcaaccgt
     1501 catttccacg attgcgactt ttattgtgtt aatggtgtat ttgcgaaaac gaaaccatcc
     1561 gctgcaattt gataaaacag tgcgccgtta tttgaaaatg gataaagaac tgctggctct
     1621 cctgctgcgc cttgggattc catcaagcat caatatgatt ctcgtctcat tatcagagat
     1681 agcggttatt tcattcgtca accattatgg ctctaatgcg acagctgcat acggcgtggt
     1741 caaccaggta gccagctatg tgcagatgcc ggccgtcagc cttggtattg cggtgtctat
     1801 attcgccgcg caatcgatcg gagcgaacga gtttggccgc ctaaaacagg taattcgtgt
     1861 cgggatttgg ctgaattata tcattggcgg tgtgctgatc attctgatct atgttttttc
     1921 acatcagatt ttaggattat ttttaacaga acaagagacg ctttacattg cgcatcgtct
     1981 cttgatgatt acattgtgga gttacttact ctttggaaat gcccaaatta tttctgcgac
     2041 gatgcgggca agcggaacgg ttttatggcc gaccgttatc agtatttttg ccatctgggg
     2101 agtagaggtg cctgtcgcgt tcgtactttc ccactacaca aatcttgaaa tactcggcgt
     2161 ctgggttggc tatccggctg cgtttgccgt cagcttgctg ttgatttatg ggtattatca
     2221 attcgtttgg aaaaagaaac agattacacg tctcatccaa tagcatcccg aatcacaccg
     2281 ttcaacaatt gaacggtgtt ttttgtttga taaagagaaa acataaatgg aggaaatatg
     2341 gcacattaca ttgaattctt atacaagcaa tgaatcaagg agaggttgag tacacatggc
     2401 aaacgatatt gagaagatta cgtatttagg aacacctgcc atcaaagcag gcaacgaaca
     2461 tatagaaatg attgtcgttc ccgagtgggg gagcaacgtc atttctcttg tggacaaaac
     2521 gacacagctg cagcttttgc gtgaaccgga aacagcagaa agctttcatg atacaccaac
     2581 attgtatggc attccgatcc tgtttccgcc taaccggata agtgacggta cattcagttt
     2641 ccggggcaga acatatcatt ttgatatcaa tgaaaaggat aaacacaacc atcttcatgg
     2701 gtttctttat caggaaaaat ggaacgtagt gaccatgaag caaactgatg aagaagtgat
     2761 cgttgaaact gaaattgacc tttcagagct tccccatgtg tataagcaat ttccgcatca
     2821 tgccgtcgtt cggatgacat acacgctaaa ggggaatact ctatttaaac atgcgacggt
     2881 catgaacaaa ggaaaggaag cgtttccttg gggaatcggc tatcatacta catttgtatt
     2941 tccagaagaa agctcgttat tctccctgac agcagatcag caatgggagt tggatgagcg
     3001 cctgctgccg accgggaaac tgatggatgt acagaataaa gaagccttgc atgaagggat
     3061 ggatttacgc agccagcagc tggatgatgt ctttttatct tcttatcaga aaaggggcgg
     3121 agaaaatcag gcagtcattt atcatcagca tgcacatata agcattattt ataaggcaga
     3181 tgaacaattt aaacattggg ttgtatacaa cgcggacgga aagcagggat atttatgccc
     3241 tgaaccttac acatgggtga caaacgctgt gaatcttaat gtgtctccat cactcactgg
     3301 tgtacaagtg ctggagccgg gagaagaaac gacagccaaa agcagtatca ccattgaact
     3361 gaatcatcaa tagccaatga tgaggcgcag agaggcgggg taaaaaaatg aaaaaaagca
     3421 taaagcttta tgttgctgtt ttgctgcttt tcgtcgttgc ttcggtacct tatatgcatc
     3481 aggctgcgca agcggctgaa aaacaaggtg cacttgccgg gcagatcgat aagattttag
     3541 ctgatcatcc tgcactcgaa ggcgcaatgg ccggcatcac cgttcgttct gcggaaacag
     3601 gcgctgttct atatgagcat tcaggagaca tccgaatgag gcccgcctct tctttaaaac
     3661 ttttgacagc ggccgcatca ctttctattc tcggagagga ctattccttt acaaccgagg
     3721 tccggacgga tggcaaattt aaagggaaaa agctgaatgg aaacctttat ttgataggga
     3781 aaggagatcc gacactttta ccatctgatt ttgaagaaat ggctgagaaa ttgaaacaat
     3841 ccggcgtgaa agagatcaaa ggcaatctga tcggtgatga tacgtggtat gatgacatcc
     3901 gattatcgcc tgatatgccg tggagtgatg aatacacata ttacggcgcg caaatctccg
     3961 ctttaacagc gtctccgaat gaagattatg atgcaggcac tgtgattgta gagatcaccc
     4021 cgggtaaaaa agagggggaa aagcctgccg tttctgtatc tccgaagacg gattatgtaa
     4081 cgattaagaa cgatgccgaa acgactgcgg ctggccaaaa gaaagacctc acgatcgaaa
     4141 gagagcatgg aacaaacacg atcacaattg aagggagcgt accggttgac gcaagcaaga
     4201 caaaggaatg gattgctgtc tgggagccgg ctggatacgc tttggattta tttaagcagg
     4261 cactaaaaaa acaaggaatt actgtaaaag ggcacgtcaa gacaggcgct gcgcccggtt
     4321 cttcagacgt gttaatctct catcgctcaa tgcctctgtc aaaactattt gttccgttta
     4381 tgaaattaag caataacgga catgccgaag tgctcgtaaa agagatgggg aaagtgaaaa
     4441 aagaagaagg aagctgggaa aagggacttg aagtattaaa caatgcgctt ccggaatttg
     4501 gtgttgatcc caaatcactt gtcttaagag acggatcagg tgtctcccat attaatgctg
     4561 tatcctcaga tcaaatctca cagctgttat atgacattca agatgagaaa tggttctcag
     4621 cttatctaaa ttctctgccg gttgcgggca atagtgacag gatgataggc ggaacactga
     4681 ggaaccggat gaaagacact cctgcacaag gaaaggtaag ggcaaaaacc ggatcgttaa
     4741 gcacagtcag ctctttatcc gggtacgcag aaaccaagag cggaaaaacc cttgtttttt
     4801 ctattctttt gaacggattg attgatgacg aggatggaaa agatatcgag gatcaaatcg
     4861 ctgtcatctt ggcaaatcaa tcatagcgtc agacctgttt tccaaggaaa acaggttttt
     4921 ttatgtcttc aataatattc cggtcgcatc tgccaacaaa tagaaagatt acgcgcaaca
     4981 gcaaattagc acaattgaaa aattagtgtg taagacccga atcagaatga tacttttatc
     5041 actttatatt aaaaaatttg atgaagaaag aaaccaaaac taaagaaata atactatatt
     5101 caaaaaaatg ttataaaaat ttaatatttt ttattgtact ttaaaaaata tcctttaata
     5161 tagtgcatat atggattata tagtcatata atttctttta ttgtcatttt tctacttttt
     5221 tcttactctc ttattatgag aactggaggg aatccgttga gccaacatac ttattcttta
     5281 acccatgccc aaaggagagt gtggtttacc gaactgctgg agccaggtac cagcatttgc
     5341 aatcttacgg cctgtgtgaa attcagaggc aatattgatc ttgatgttct agagggcgcg
     5401 ctgaatcatt ccatctcccg caacgacgcc atcaggtttc agttgctaga aggagaagag
     5461 cttgagccgc gattgtacct tactgaatat aaatatactt cactgggaat aatagatttt
     5521 tcaaatgttg aaatgataga aatagagcaa tggatacaag aacaagcgag aatcccattt
     5581 aaactattca actcgcctct ttatcaattt tatctcctta gaatccacaa tcatgaggtt
     5641 tggctgtttg ctaaattcca tcatattata atggacggca tctccttaaa cgtgatggga
     5701 aatcaaatta tagatcttta tcaaaaaatg aaaaagcaag atcctttacc cgatcagcct
     5761 gaaccttctt acttgagcta catcgaaaaa gagagtcaat atttacagtc ttcacgtttt
     5821 acaaaggacc gtttattttg gactcaaacc tttcagaatc cgccgggata ccactcgtta
     5881 gccggccaga cgtctttaca aaaacagagc acatctgcat ccagagacac catcaccctt
     5941 tcacctcatc tagggcaaac gattcgaatc ttttgcgaag aacaaaagat caatatcatt
     6001 tccttattta tggcttcatt ttacatatgc atcagccgga tcacttctaa aaaagacctt
     6061 gccattggca cctactatgg caacagaggg tcaaaggctg aaaaagaaat gctcggcatg
     6121 ttcgtcagtt ctcttcccat cagaataacg gttgatcctg acgcggactt tctatccttt
     6181 gtccgcagag taggcaggga acagctttcc gttatgcgtc accaacggtt tccttataat
     6241 ttgctggtaa atgaattaag aaaagaacag aaggatttac acaaccttat cggtatatct
     6301 atgcagtatc aaccgctcca atggcacaat gcagacggtt ttgactatga aacggcgctt
     6361 tatttcagcg gatacaccgc gaccgagctt tctgttcaaa tacaggaacg gacagacacc
     6421 ggaactattc aattaaattt cgactatcaa aataccttgt tttctcttga ggatattaag
     6481 cgaatacaga accatctgct tacgttactg gaaaacgccc ttcaacatcc gcacagtttc
     6541 ataaaagaat tagatatgac taatataaga gagaaacaaa aattgttgta tgagtttaat
     6601 aagacggaag ctgtttcgcc tgaagcccca accctgcacg gtctgtttga acgacaggcg
     6661 gctgtcacac cggagcggcc agccatccgc ttttccagcg attcgctgac gtacgctgag
     6721 cttgacatgt atgccagccg gctggctgcg cgcctcgcgg cacgcggcgt cacgaaggaa
     6781 agcatcgtcg gtgtcctgtc tgaacggtca cctgaaatgc tgatagcggt acttgctgtc
     6841 ttgaaggca
//
