LOCUS       NZ_JALAVX010000039      1610 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus subtilis strain EC49O2N-C8 contig_0000039, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAVX010000039
VERSION     NZ_JALAVX010000039.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.01322621364343x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:42:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,133
            CDSs (total)                      :: 4,055
            Genes (coding)                    :: 3,871
            CDSs (with protein)               :: 3,871
            Genes (RNA)                       :: 78
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 97 of 184
            Pseudo Genes (incomplete)         :: 98 of 184
            Pseudo Genes (internal stop)      :: 21 of 184
            Pseudo Genes (multiple problems)  :: 27 of 184
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:11:08
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1610
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAVX010000039.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1610
                     /db_xref="taxon:1423"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis"
                     /strain="EC49O2N-C8"
                     /submitter_seqid="contig_0000039"
     gene            <1..>1610
                     /locus_tag="MOE40_RS19775"
                     /old_locus_tag="MOE40_19740"
     region          1..1610
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1610
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1610
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>1610](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>1610
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>1610
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (302-371). E-value: 4.4e-25. Score:
                     79.7. Matches aSDomain: nrpspksdomains_MOE40_RS19775_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (385-535). E-value:
                     3.9e-52. Score: 169.1. Matches aSDomain:
                     nrpspksdomains_MOE40_RS19775_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389716.2"
                     /locus_tag="MOE40_RS19775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE40_19740"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268550233.1"
                     /sec_met_domain="AMP-binding (E-value: 2.6e-72, bitscore:
                     229.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 6.1e-18, bitscore:
                     51.4, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.7e-53, bitscore:
                     167.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAYLLPPGWEKDPALIVQAIHQEKVTTAHFIPAMLNSFLDQAEM
                     LALGDGTSLKRVFAGGEPLAPRTAARFASVLPQVSLIHGYGPTEATVDAAFYVLDPERD
                     RDHLRIPIGKPVPGARLYVLDPHLAVQPSGVAGELYIAGAGVARGYLNRPALTEERFLE
                     DPFYPGERMYKTGDVARWLPDGNIEFLGRLDDQVKIRGYRIEPGEIEAALRSIEGVREA
                     AVTVRTDSGEPELCAYIEGLRRNEVRAQLERLLPGYMIPAYMIEMEQWPVTPSGKLDRN
                     ALPAPGGAADAETYTAPRNVTEMKLSQLWEDVLKNGPVGIHDNFFDRGGHSLKVTALVS
                     RIAKEFDVQVPLKDVFAHPTVEGLATVIREGTDSPYEAIKPAEKQETYPVSSAQKRIYV
                     LQQLEDGGTGYNMPAVLELEGKLNPERMDRAFKELIKRHESLRTSFEQDAGGDPVQRIH
                     DEVPFTLQTTVLGARTEQEAAAAFIKPFDLSQAPLFRAQIVKVSDERHLLLVDMHHIIS
                     DGVSVNILIREFGELYNNR"
     CDS_motif       394..483
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE40_RS19775_0001"
                     /evalue="2.90E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE40_RS19775"
                     /protein_end="161"
                     /protein_start="131"
                     /score="57.9"
                     /tool="antismash"
                     /translation="PSGVAGELYIAGAGVARGYLNRPALTEERF"
     CDS_motif       565..630
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE40_RS19775_0002"
                     /evalue="2.50E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE40_RS19775"
                     /protein_end="210"
                     /protein_start="188"
                     /score="41.7"
                     /tool="antismash"
                     /translation="FLGRLDDQVKIRGYRIEPGEIE"
     aSDomain        907..1113
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE40_RS19775_PCP.1"
                     /evalue="4.40E-25"
                     /label="MOE40_RS19775_PCP.1"
                     /locus_tag="MOE40_RS19775"
                     /protein_end="371"
                     /protein_start="302"
                     /score="79.7"
                     /tool="antismash"
                     /translation="EMKLSQLWEDVLKNGPVGIHDNFFDRGGHSLKVTALVSRIAKEFD
                     VQVPLKDVFAHPTVEGLATVIREG"
     aSDomain        1156..1605
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE40_RS19775_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.90E-52"
                     /label="MOE40_RS19775_Condensation_LCL.1"
                     /locus_tag="MOE40_RS19775"
                     /protein_end="535"
                     /protein_start="385"
                     /score="169.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="ETYPVSSAQKRIYVLQQLEDGGTGYNMPAVLELEGKLNPERMDRA
                     FKELIKRHESLRTSFEQDAGGDPVQRIHDEVPFTLQTTVLGARTEQEAAAAFIKPFDLS
                     QAPLFRAQIVKVSDERHLLLVDMHHIISDGVSVNILIREFGELYNN"
     CDS_motif       1228..1335
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE40_RS19775_0003"
                     /evalue="2.30E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOE40_RS19775"
                     /protein_end="445"
                     /protein_start="409"
                     /score="55.3"
                     /tool="antismash"
                     /translation="YNMPAVLELEGKLNPERMDRAFKELIKRHESLRTSF"
     CDS_motif       1534..1599
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE40_RS19775_0004"
                     /evalue="1.70E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOE40_RS19775"
                     /protein_end="533"
                     /protein_start="511"
                     /score="33.3"
                     /tool="antismash"
                     /translation="MHHIISDGVSVNILIREFGELY"
ORIGIN
        1 gcttcggcgt atctgcttcc gcccggctgg gaaaaagacc ccgcgttgat cgtacaagcc
       61 attcatcagg aaaaagtgac aacggctcat tttattccgg ctatgctgaa cagctttctc
      121 gatcaagcgg aaatgttagc gctcggtgac gggacaagcc tgaagcgtgt attcgccgga
      181 ggtgaaccgc ttgcgccgcg tacggcagcc cgttttgctt ctgtattgcc gcaggtatca
      241 ttaatccatg gctacgggcc gacagaagca acggtagacg cggcatttta cgtattggat
      301 ccagaacggg acagagatca tttgcggatt ccgattggga agcccgtacc cggtgcgcgt
      361 ttgtatgttt tagatccgca tttagccgta cagccttccg gtgtcgccgg cgaactatac
      421 attgccggag cgggtgttgc cagaggttat ttgaatcggc cggcattaac ggaggagcgt
      481 tttcttgagg acccgtttta cccgggtgaa cgtatgtaca aaacagggga tgtggcgcgc
      541 tggctccctg acgggaatat tgaattcctc gggcggttgg atgatcaggt gaaaatccgc
      601 ggctaccgga ttgagccggg agagattgaa gcagcactaa gaagtataga aggcgtaagg
      661 gaggcggccg ttaccgtacg gacagacagc ggcgagccgg aattatgcgc ttatatagaa
      721 gggctcagaa gaaacgaagt gcgggcacag cttgagagac tgttgccggg ctacatgatc
      781 ccagcctata tgatagagat ggaacagtgg ccggttacgc caagcggaaa gctcgaccga
      841 aacgccctgc cagctcctgg cggagcggca gatgcggaga cctacacagc gccgagaaac
      901 gtgactgaga tgaagctttc tcagctgtgg gaggacgtgc tgaaaaatgg ccctgtcggg
      961 attcacgaca acttttttga ccgcggggga cattccttaa aagtaacggc gcttgtctca
     1021 cggattgcca aagaattcga tgtacaggtg ccgctaaaag atgtgtttgc ccatccgacg
     1081 gtagaaggac tggctaccgt tatccgtgaa ggaacggaca gtccatatga agcgattaag
     1141 cccgctgaaa aacaggaaac ctatccggtt tcctctgccc aaaagcggat ttatgtcctt
     1201 cagcagctgg aggacggagg gacaggctac aatatgccgg ctgtgttaga gctcgaaggg
     1261 aagctcaacc ctgaaaggat ggacagagct ttcaaagagc tgatcaagcg ccacgagtcg
     1321 cttcggacct ccttcgaaca ggacgcagga ggcgatccgg tgcagcgcat ccatgacgag
     1381 gtgccgttta cgttacagac tacagttctc ggcgcacgaa ccgaacagga ggccgcagct
     1441 gcatttatca aaccgtttga tctcagccaa gcgccgctgt tccgtgccca aatcgtaaag
     1501 gtatcagatg agcggcacct gttgctggtt gatatgcatc acatcatttc agacggtgtt
     1561 tccgtcaaca ttctgattcg ggaattcggc gagctgtaca ataatcggaa
//
