LOCUS       NZ_JALAVQ010000016     12765 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus atrophaeus strain TP10O4N-G7 contig_0000016, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAVQ010000016
VERSION     NZ_JALAVQ010000016.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 43576)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 43576)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 345.71764523836856x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:42:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,242
            CDSs (total)                      :: 4,158
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 92 of 169
            Pseudo Genes (incomplete)         :: 113 of 169
            Pseudo Genes (internal stop)      :: 22 of 169
            Pseudo Genes (multiple problems)  :: 50 of 169
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:20:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 12765
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAVQ010000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12765
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..12765
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12765
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[875:2765](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(876..2765)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(34..879)
                     /locus_tag="MOE59_RS20500"
                     /old_locus_tag="MOE59_20420"
     CDS             complement(34..879)
                     /EC_number="1.15.1.1"
                     /GO_function="GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389815.1"
                     /locus_tag="MOE59_RS20500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20420"
                     /product="superoxide dismutase"
                     /protein_id="WP_003325798.1"
                     /transl_table=11
                     /translation="MKRHTYQTEMINWCEDLKEQVQKRGQYNHFEAQFKKVFDKLEDEQ
                     ATEEEWYREAAALYRDITEADGTSERKTYVPIGKHVLPKLPYKYSALEPYISREIMTLH
                     HTKHHQSYVDGLNKAELELKKARQTKNYELVAHWERELAFHGAGHYLHSIFWFSMHPNG
                     KRRPTGALFQMIDLSFGSYSAFKEHFTQAAKKVEGVGWAILVWSPRSGRLEILAAEKHQ
                     LFSQWDVIPLLPLDVWEHAYYLQYKNERAKYVDNWWNVVDWREAEKRFEKARNVVWQLY
                     "
     gene            complement(876..2765)
                     /locus_tag="MOE59_RS20505"
                     /old_locus_tag="MOE59_20425"
     CDS             complement(876..2765)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015388120.1"
                     /locus_tag="MOE59_RS20505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20425"
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="WP_268489381.1"
                     /sec_met_domain="T2TS (E-value: 2.3e-115, bitscore: 377.8,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTLQEQVARCQKKTITQLKNMQNADGSWSFCFEGPIMTNSFFIL
                     LLTSFEDKGNEELIVKLAKGIREKQRPDGTFTNYSGEEKGNVTATVQGYVGMLASGCYL
                     RSDPHMIQAERFIISQGGLKDVHFMTKWMLAANGLYPWPALYLPLCLLAIPPTFPLHFY
                     QFSTYARIHFVPMAVTLNQRFFLKNPNIPSLKYLDSHMSKNPFTWLRTDAYDQRDLSSI
                     LAYWKQLLNVPFSLHQLGFRTAKTYMLDRIEKDGTLYSYASATIFMIYSLLSLGMSRHS
                     SIIQQALNGVKTLVTECDGIPYLENSTSAVWDTALISYSLQQSGVPEKDLMITGAADFL
                     LENQHKKKADWSVKNPHAAPGGWGFSKINTNNPDCDDTAAALQAIPRSLSPMSWDRGLN
                     WLLSMQNKDGGFSAFERNVNHPLIRLLPLESAEDAAVDPSTPDLTGRVLRFLGEKAGMT
                     QDNRQITKAVNWLIDNQEDNGSWYGRWGVCYIYGTWAAITGMRACGTEAFHPAVKKAVH
                     WLKTIQQDDGGWGESCRSAEVKTYVPLRYGTVTQTAWALDALLQYEEPDHPSITKGLGF
                     LINSENHNKHSFDYPTGIGLPKQFYIRYHSYPFAFSLLAYSSYMKSIEKREMK"
     gene            complement(2875..4362)
                     /locus_tag="MOE59_RS20510"
                     /old_locus_tag="MOE59_20430"
     CDS             complement(2875..4362)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389813.1"
                     /locus_tag="MOE59_RS20510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20430"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_003325800.1"
                     /sec_met_domain="Aldedh (E-value: 7.5e-194, bitscore:
                     636.1, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSLTMQISKRLETFLQGTKKLYIDGKFVPSASGATFVTPNPATG
                     ETLMTLFEAQAEDVDKAVKAARKAFDQGEWRTMPAASRSRLMYKLADLMEEHQTELAQL
                     ETLDNGKPISETTNGDIPLAIEHMRYYAGWSTKITGQTIPVAGSYFNYTKHDPVGVVGQ
                     IIPWNFPLLMAMWKMGAALATGCTIILKPAEQTPLTALYLAELIDEAGFPDGVINIIPG
                     FGETAGEALSNHEEVDKIAFTGSTEIGKKIMETAAKSIKRVTLELGGKSPNIILPDANL
                     KKAIPGALNGVMFNQGQVCCAGSRVFIQKDRYDEVVSEMVTYAESLRQGAGLHKDTQIG
                     PLVSKEQHERVLSYIQKGKDEGAKAVTGGSCPYEEGYFVSPTVFTDVEDGMTIAKEEIF
                     GPVLTAIPYDTVDEVIERANNTEYGLAAGLWTENLKHAHYIADRLQAGTVWVNCYNVFD
                     AASPFGGYKQSGLGREMGSYALNNYTEVKSVWINLED"
     gene            4594..4797
                     /locus_tag="MOE59_RS20515"
                     /old_locus_tag="MOE59_20435"
     CDS             4594..4797
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389812.1"
                     /locus_tag="MOE59_RS20515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20435"
                     /product="DUF6501 family protein"
                     /protein_id="WP_003325801.1"
                     /transl_table=11
                     /translation="MIHKTWLKKETVRKVKCVQTNAKKYIVNRVLTPGKEYEVKNETEE
                     FLFVVDNSNKVGGFYKEYFEEV"
     gene            4867..5010
                     /locus_tag="MOE59_RS20520"
                     /old_locus_tag="MOE59_20440"
     CDS             4867..5010
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003325802.1"
                     /locus_tag="MOE59_RS20520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20440"
                     /product="DUF4025 domain-containing protein"
                     /protein_id="WP_010788920.1"
                     /transl_table=11
                     /translation="MKRQVNNGKEEAENFPEQKHPTEGEDVTREQISDTYYEGTIEDTD
                     RD"
     misc_feature    complement(5077..5091)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.9"
                     /tool="antismash"
     misc_feature    complement(5096..5110)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 22.7"
                     /tool="antismash"
     misc_feature    complement(5097..5110)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     gene            5343..5687
                     /locus_tag="MOE59_RS20525"
                     /old_locus_tag="MOE59_20445"
     CDS             5343..5687
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389811.1"
                     /locus_tag="MOE59_RS20525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20445"
                     /product="PH domain-containing protein"
                     /protein_id="WP_003325803.1"
                     /transl_table=11
                     /translation="MGIFSVSRPTDAKKLEALLVEGERIESIYKLRVDQICFTNKRIIF
                     FDNKMFSKKKVRVFLPYKTIESFAIQEAGMFDPDTGLLLMTRSKTFDLEFAKDTDLSEV
                     QAILTKYLCS"
     gene            5831..6322
                     /locus_tag="MOE59_RS20530"
                     /old_locus_tag="MOE59_20450"
     CDS             5831..6322
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389805.2"
                     /locus_tag="MOE59_RS20530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20450"
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="WP_010788918.1"
                     /transl_table=11
                     /translation="MALTKEQTQHLYHKLLDLQKELSGPKKATESMTEEAGELSNGVDN
                     HMADHGSLYTDRMTDQTVKEVDRELLDEVNNALQKIKDGTYGICEATGEEIPYERLDAV
                     PYARMTVEAQTDREEALEPDAPAVEKEFHAQVKDLSNKETIDQKSSATYEVLDREQDSK
                     "
     gene            6477..7103
                     /locus_tag="MOE59_RS20535"
                     /old_locus_tag="MOE59_20455"
     CDS             6477..7103
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389804.1"
                     /locus_tag="MOE59_RS20535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20455"
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="WP_003325805.1"
                     /transl_table=11
                     /translation="MSQVLFVKASDRTAEEGVSVKLYEAFLAAYKENNPNDEVIELDLH
                     KENLPYLGRDMINGTFKAAKGIEMSEEEKKQASTADVYLNQFVKADKVVFAFPLWNFTV
                     PAVLHTYVDYLSRAGVTFTYTPEGPKGLMGDKKVALLNARGGVYSEGPMAALEMSLNFM
                     KTVLGFWGVQDLHTVVIEGHNAAPDNAQTIVEKGLQEAKDLAAKF"
     misc_feature    7257..7273
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.04"
                     /tool="antismash"
     ncRNA           7308..7494
                     /db_xref="RFAM:RF00013"
                     /gene="ssrS"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /locus_tag="MOE59_RS20540"
                     /ncRNA_class="other"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /old_locus_tag="MOE59_20460"
                     /product="6S RNA"
     gene            7308..7494
                     /gene="ssrS"
                     /locus_tag="MOE59_RS20540"
                     /old_locus_tag="MOE59_20460"
     gene            7627..9405
                     /gene="recQ"
                     /locus_tag="MOE59_RS20545"
                     /old_locus_tag="MOE59_20465"
     CDS             7627..9405
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003325807.1"
                     /locus_tag="MOE59_RS20545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20465"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_003325807.1"
                     /transl_table=11
                     /translation="MLEKARSLLAHYFGYEKLRPGQEQAIRSVTEAKKNTACIMPTGGG
                     KSICYQIPALLFEGTTIVISPLISLMKDQVDALEEAGIAAAYINSTQSNQDIQSRLTAL
                     KNGSYHLFYITPERLTSPEFIRILQSITVPLVAIDEAHCISQWGHDFRPSYRNIEILFR
                     ELKEKPVIMALTATATPEVHEDICKQLHIEKENTVYTGFSRDNLTFKVAKGENKDRFIE
                     EYLNRNKLEAGIIYTATRKEADRICEKLKRQHINAGRYHGGLPDEVRKEQQEQFLNDQI
                     QVMVATSAFGMGIDKSNIRFVLHYQIPKDMESYYQEAGRAGRDGLDSECILLFSPQDIM
                     VQRFLIEQSASDEGKQKQDLKKLRQMVDYCHTEDCLQRFILKYFGEVDPAACGQCGNCT
                     DTRQAHDVTKEAQMVLSCMIRMNERFGKTLIAQVLAGSKNKKVLDNGFSRLSTYGILKH
                     QSVSEISDFIEFLISDDFIQMSDGTFPTLFVTNKGRNVLLGKEDVVRKEALKAAAITEN
                     DALFERLRMLRKQIAAEQGVPPFVVFSDQTLKEMSGKQPANEEDLLSIKGVGEQKRVKY
                     GKLFLEEIQAYVSETV"
     gene            9653..10558
                     /locus_tag="MOE59_RS20550"
                     /old_locus_tag="MOE59_20470"
     CDS             9653..10558
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389802.1"
                     /locus_tag="MOE59_RS20550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20470"
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="WP_268489383.1"
                     /transl_table=11
                     /translation="MKKTIMSFVAVAAISSTAFGAHASAKEITVQKGDTLWGISQKNGV
                     NLKDLKEWNQLSTDIIIAGEKLTISSEEETTAGQYTVKEGDSLWKIAQKYGISINSLKS
                     WNNLDSDMIYAGTTLAVKGQVSASNTAQESQPQAEPKQEAVQTEQPKQETVQQQPKQET
                     VHQQPKQETVQQQPKQESKAAAETSEKTEEQPVQSNTSNQNAKKELTVTATAYSANDGG
                     ISGVTATGVDLNKNPNAKVIAVDPKVIPLGSKVYVEGYGEATAADTGGAIKGNKIDVFV
                     PKTSDAHNWGVRTVKVKVLN"
     gene            10739..12328
                     /locus_tag="MOE59_RS20555"
                     /old_locus_tag="MOE59_20475"
     CDS             10739..12328
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019714632.1"
                     /locus_tag="MOE59_RS20555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_20475"
                     /product="solute:sodium symporter family transporter"
                     /protein_id="WP_268489385.1"
                     /transl_table=11
                     /translation="MLWIIVSFLLINIGIFLFSWLRTKDVNLHTSDGYFLGGRSLTALY
                     IGSSLLLTNLSTEQMVGLNGQSYAGSMTAMSWEASAPIALIFFALLFLPRYLKTGITTI
                     PDFLEKRFDQKTRQIVSLLLIIGYAVSFLPTVLYSGALVLDQVFGFTDNFGISTFTAVL
                     IISFIIGITSCCYIFFGGLKAAASADAIYGIGLICGGLLIVVLGILAIGEGNFGGGVAK
                     LTAVHTEKLNSIDISNSAEVPWPTILTGLLVNNLFYWAVNQAIVQRALGAKNLAEGQKG
                     ALLAGFFKLVGVFYLVLPGIIAFHLYGGGINNADSVYPRLIVDLLPTALTGIFAAILFG
                     AILSSFNGSLNSTITLFTLDIYKPIFKPNANDADLVKTGRIFVAILGVVSIIIAPFILY
                     APSGLYNYLQEMFGFFNVPILAAVVVGFFSKRVPALAVKIAIPAHIVLYGLSKIFLGHI
                     HFLYILAVLFPFSVAVMLFVGRLRPREKEYRLNHDPQVDLKPWKHVKLASCIILVMVSV
                     IYIVFSPWGIAG"
ORIGIN
        1 tcttcggggg tcagttcctc atcgggggct tttttaataa agctgccata ctacgtttcg
       61 cgctttttcg aaacgttttt ccgcctcgcg ccaatcaacg acattccacc agttatcaac
      121 atattttgcc cgctcatttt tatattgtaa ataataggcg tgttcccata catcaagggg
      181 cagtaagggg atgacgtccc attggctgaa aagctgatgc ttttcagcag ctaggatctc
      241 aagccgtccg gaacgcggcg accaaacgag tatggcccag ccgacacctt ccactttttt
      301 agccgcttgt gtgaagtgct ctttgaaggc agaatagctg ccgaacgaca aatctatcat
      361 ttgaaacaat gctccggtag gacgcctttt tccgttcgga tgcatcgaga accaaaaaat
      421 gctgtgcaaa taatgtcccg ctccatgaaa agcaagctct cgttcccagt gggcgactaa
      481 ctcatagttt ttcgtttgtc ttgctttctt tagctccagt tctgctttat ttaaaccgtc
      541 cacatagctt tgatggtgct ttgtatgatg aagtgtcata atctcacgag aaatataggg
      601 ttcaagtgcc gaatacttgt aaggaagctt tggcagaaca tgctttccaa tcggcacgta
      661 ggtttttctt tcgcttgttc cgtctgcctc tgttatatct ctgtataatg cggcggcctc
      721 ccggtaccat tcttcttctg tcgcttgttc atcctcaagc ttatcaaaaa cttttttaaa
      781 ttgagcctca aaatgattat attgccctcg tttttgaacc tgttctttca aatcctcaca
      841 ccaattgatc atctcggttt ggtacgtatg gcgtttcatt tcatctccct cttttcaata
      901 gatttcatat agcttgaata agccagtaaa gaaaaggcaa aggggtagct gtgataccga
      961 atataaaact gttttggcag tccgattcct gttggatagt caaaactgtg cttgttgtga
     1021 ttttcgctgt taataagaaa cccgagtcct tttgtaatag acgggtgatc aggttcttca
     1081 tattgcaata aagcatctaa cgcccaggct gtttgggtta ccgttccata tcgtaaaggt
     1141 acgtatgttt tgacttcggc gcttctgcag gattcacccc agccgccatc atcctgctga
     1201 attgttttca gccaatgcac cgcttttttt acagcagggt gaaacgcttc tgtaccgcag
     1261 gcacgcattc ctgttatagc ggcccatgtt ccgtaaatat agcaaacgcc ccatctgccg
     1321 taccatgatc cgttatcctc ctggttatcg atgagccagt tgacggcttt tgttatttgc
     1381 ctgttatcct gtgtcatacc tgctttttca cctaagaagc ggagcacgcg tccggttaag
     1441 tcgggtgtcg aaggatcaac cgctgcgtcc tcagccgact caagcggaag cagacggata
     1501 agagggtggt tcacatttct ttcgaaggct gagaatcctc cgtctttgtt ttgcatagac
     1561 aatagccagt tcagcccccg atcccaagac ataggagaca gggagcgcgg aatcgcttgc
     1621 aatgcggctg cggtatcatc acaatcagga ttattggtgt tgatttttga aaagccccag
     1681 ccgccgggtg cagcgtgcgg atttttgaca gaccaatctg ctttcttttt atgctgattt
     1741 tccaacagaa agtctgcggc tccggttatc atcaaatctt tctcaggcac accgctttgc
     1801 tgaagggaat agctgattaa agcggtgtcc caaacagcag atgtggaatt ctctaaataa
     1861 gggatgccgt cacattccgt aaccaacgtt ttaaccccgt tcaaagcctg ctgaatgatg
     1921 gaagaatggc gtgacatgcc aagggacaga aggctgtaga tcataaaaat ggtcgcgctg
     1981 gcatagctgt acagggtccc gtctttttct atacgatcaa gcatataggt tttggctgta
     2041 cgaaagccaa gctgatgtaa agaaaacggc acgttcagca gctgtttcca ataggccaga
     2101 atagaagaga ggtccctctg atcatacgca tctgttctga gccatgtgaa aggattttta
     2161 gacatatgcg aatcgagata tttgagagag gggatatttg gatttttcag aaagaatctt
     2221 tgattaagag tcacggccat gggcacaaaa tgaattcgcg cgtacgtgct gaactggtaa
     2281 aaatgaagcg gaaacgttgg gggaatagcc aagagacaca gcggaaggta aagcgccggc
     2341 caaggataaa gcccgttggc tgcaagcatc cattttgtca taaaatgaac atccttgaga
     2401 ccgccctgag aaataataaa acgttctgcc tgtatcatat gagggtcaga cctcagataa
     2461 catccggacg caagcattcc gacgtatcct tggacagtgg ctgtcacatt ccctttttct
     2521 tcaccggaat aattggtaaa cgttccatcc ggccgctgtt tttcccgtat gccttttgcc
     2581 aattttacaa tcagttcttc attaccctta tcttcaaatg aggtgagaag gaggatgaaa
     2641 aatgaattcg tcattattgg gccttcaaag caaaaagacc atgaaccatc agcattttgc
     2701 atgtttttca gctgtgttat ggttttcttt tggcagcgtg ctacctgctc ctgaagtgtg
     2761 ctcatcgcgt tcaactcctt cagaagcata tatattcatg ctctttcgac ggcattacag
     2821 aaagaaagaa caaaaaaact gctgtcaggg cagcagtttt tcaaaatctt ctttttaatc
     2881 ctctaagtta atccacacgc tttttacttc cgtgtaatta tttaaggcgt atgatcccat
     2941 ttctcttcca agtcctgatt gcttgtaacc gccgaatggg gatgcggcat caaacacgtt
     3001 atagcaattc acccagactg ttccggcttg caggcggtca gcgatatagt gagcatgttt
     3061 cagattttcg gtccacaatc cggctgcaag gccatattcg gtattgtttg cccgttcgat
     3121 cacttcatcg accgtatcgt atggaatggc agttaagacc gggccgaaaa tttcttcttt
     3181 tgcaattgtc atgccgtctt ctacgtcggt aaatacagtt ggtgatacaa aataaccttc
     3241 ctcatacgga cagcttccgc ctgtcacggc tttcgctcct tcatccttgc ctttttgaat
     3301 ataggaaagg acacgttcgt gctgttcttt gcttacaagc ggtccgattt gtgtatcttt
     3361 gtgcaggcct gcaccttgac gaagggattc cgcataagta accatctcac tgaccacttc
     3421 atcgtatcgg tctttttgaa taaagacccg tgaaccagcg cagcacactt ggccttggtt
     3481 aaacataaca ccgttcaagg ctcctggaat tgcttttttc aagttcgcgt caggcaagat
     3541 gatattagga gactttccgc caagctctaa tgttacgcgc ttaattgact ttgcggctgt
     3601 ttccataatt tttttgccga tttcagtgga acctgtaaag gcgatcttat ccacttcctc
     3661 atggttggac agcgcttctc ccgcagtttc tccaaatccg ggaataatat taataacgcc
     3721 gtcaggaaaa ccggcttcat caattaattc agccaagtat agcgccgtaa gcggcgtttg
     3781 ctcagcgggc tttaagataa tggtgcatcc tgtcgcaagc gcagcgccca ttttccacat
     3841 ggccataaga agagggaagt tccacgggat gatttgacca acgactccga cagggtcatg
     3901 cttggtataa ttaaaataag aacccgcaac aggtatcgtc tgtccggtta ttttagtaga
     3961 ccagccggca tagtagcgca tatgctctat ggccagcgga atatctccgt ttgttgtttc
     4021 acttatcggt tttccgttgt cgagtgtttc aagctgagcg agttctgttt gatgctcctc
     4081 catcaggtca gccaatttat acatcaatct gcttctggaa gctgccggca tcgtgcgcca
     4141 ctcaccttga tcaaaggctt ttcttgcggc ttttacagct ttatcaacat cctcggcttg
     4201 tgcttcgaag agtgtcatca acgtctcacc ggttgccgga tttggggtga caaaggttgc
     4261 tcctgaagca ctcggaacaa atttgccgtc aatgtacagt tttttggtcc cttgtaaaaa
     4321 tgtctccagc ctcttactga tttgcatcgt taaagaactc atttgtaaca aacctcctgt
     4381 aatttgtaat ggtctgcact gagattcttg gtgatagtcg attcgttggt gtgttggttt
     4441 ggaatggtta gactgcgaag tttgtgaaat tagtaagcgt atacaatgat tttataatag
     4501 agggtatagc aaaatcaatg aataaccatt attgcatttg ataagagaaa aaagctacaa
     4561 tgaacatatc gataagaaaa aggagctttt agaatgattc ataaaacatg gctgaaaaaa
     4621 gaaacagtga gaaaagtaaa atgtgttcaa acaaatgcaa aaaaatacat cgtaaaccgc
     4681 gttttaacgc cggggaaaga atatgaagtg aaaaacgaaa cagaagaatt cctgtttgtc
     4741 gtagacaatt ccaataaagt tggcggattc tataaagagt actttgaaga ggtctgattg
     4801 actgaatgaa tatgtcatgc atcatccata ataaagaatg atcaaagaaa aaagggtgaa
     4861 tgcataatga aaagacaagt caataacgga aaagaagaag cagagaactt cccagaacaa
     4921 aagcatccta cagaaggcga ggatgtcaca agggaacaaa tttcagatac gtattatgaa
     4981 ggaacgattg aagatacgga tcgggattag atgatcttaa aaccgcaggc aaagaactgc
     5041 ggtttttttc gttcagataa atggaaaata aaaccgaatt atctgaaata aacgaaattt
     5101 tttaaaaaat aatctgaaaa tgaccctgaa aaatgaaact ttcatctata tctgcacgta
     5161 ctatgttatg acagcaaata agcgcgcggt ttttaaaatg atcggatcgg gtttttagaa
     5221 aatgtaatct attcgtaatg gtttaaatga cgtccatgtc gtattcttat atagggatat
     5281 agaacttatt ttatgtactg ataataaaaa taatagataa atgcaggaag aggggttctt
     5341 aattgggtat tttttctgtt agcagaccaa ctgacgcaaa gaaacttgaa gctttgctcg
     5401 ttgaagggga aagaatagaa tctatctaca agctccgggt tgaccaaatt tgctttacaa
     5461 ataaacgaat catatttttt gataataaaa tgttctccaa aaagaaagtg cgtgtatttc
     5521 ttccttataa aacgattgaa agctttgcta ttcaggaagc gggaatgttt gaccccgata
     5581 ctggactttt gctgatgaca agaagcaaga ccttcgattt agaatttgcg aaagacaccg
     5641 acctgagtga agttcaggcg attttgacaa aatatttatg cagctaaagt gtttcacagc
     5701 ttagcggaca caccggacgt ctttatgatg gccggttttt ttgtgaaaaa gcagaggaaa
     5761 cgaaggtgtt tttgttttaa ggtttgttct ttttaaaaac gggaaagcaa gaagaaagga
     5821 gtggttacac atggcattaa ctaaagaaca aactcagcat ctctatcata aattgctaga
     5881 tctgcaaaaa gaattgtccg gcccgaaaaa agcaacggag tctatgacag aagaagcggg
     5941 tgagctgtct aacggagtgg ataaccatat ggcagaccac ggcagcctgt atacggacag
     6001 aatgacagac caaacggtaa aggaagtaga cagggaacta ttagatgaag taaacaatgc
     6061 tttacaaaaa ataaaagacg gaacttatgg aatatgtgaa gcgactggag aagaaatccc
     6121 ttatgagagg ctcgatgctg tgccgtacgc aaggatgact gttgaagctc agactgacag
     6181 ggaagaggct ctagagcctg atgcacctgc ggttgaaaag gaatttcatg cacaggtgaa
     6241 agacctttcg aataaagaaa cgattgatca aaaaagctcc gctacgtacg aagttttaga
     6301 tcgggagcag gactcaaaat aaaagtttca agaaggcgag tacaggcttt tgcagtactt
     6361 gccttttttt tcgtaaaaaa acctcgaatt cgaaataaat ggattgacat tatttaaaaa
     6421 agaaagtaac atactatttg taagtaaata taaaaagatt accgggagga acaaaaatgt
     6481 ctcaagtctt atttgttaaa gcaagcgatc gtacagctga agaaggtgtt tcagtaaaat
     6541 tatatgaagc tttcttggct gcttataaag aaaataaccc aaatgatgaa gtgatcgaac
     6601 tggaccttca taaagaaaac cttccttacc ttggaagaga tatgattaat ggaacattca
     6661 aagccgctaa aggcattgaa atgtcagaag aagagaaaaa acaagcgtca acggctgacg
     6721 tttatctgaa ccaatttgta aaagctgata aagtcgtttt tgcattcccg ctctggaact
     6781 tcacagtgcc tgctgtgctt catacttatg ttgattacct atctcgcgcg ggtgttactt
     6841 tcacatatac gccagaaggt ccaaaaggct taatgggaga caaaaaagta gcccttctta
     6901 acgcacgcgg gggtgtatat tctgaaggac cgatggctgc tcttgaaatg tctcttaact
     6961 tcatgaaaac agttcttggc ttctggggcg tacaagattt gcataccgtg gtgattgaag
     7021 gccataatgc agctccggat aacgcacaaa caatcgttga aaaaggccta caagaagcaa
     7081 aagaccttgc tgctaaattc taatattgat ggaaactccc gtgcttttta tagccggggg
     7141 tttttatttt atggaaattt atctctattt tattatattt atataatgaa aaaactgaat
     7201 gtgttaacgc tgtctcagta aagtgtcttt tctgagctct gaaaaaagtt tttttattgt
     7261 aatatcataa ccacttgaaa aaatgtcagt aatcgtgtat tctttaatag aagctacatt
     7321 gttcgtattg ataattaagt tttaaccttt aagctgtaat agggagtttt acatcgaagc
     7381 cggaatgtca tgcctccaag atagaggacg acaggaacgc ttctgcaaac acccactttg
     7441 aggagtggtg gtttaaaact ttcttacgtg catacggcaa tacgtagcga aagccatttc
     7501 cttttggaga tggcttttta ttttgcgttt ttcttttgga aattcaattt cagccgacca
     7561 gtaatagcaa ggcttctttt tatgataaga tgggagaaac gaagaaaaag cgaggtctta
     7621 gacgttatgt tagagaaagc ccgatcgcta ttagcgcatt attttgggta tgagaagctg
     7681 cgccccggtc aggagcaggc gatacggtca gtgacagaag ccaaaaagaa cacggcctgt
     7741 attatgccga cgggcggcgg aaaatctatt tgttatcaaa ttccggcttt gctatttgaa
     7801 ggaacgacga ttgtgatttc gcctttgatt tccttaatga aagatcaggt cgatgccctt
     7861 gaggaagccg gaatcgcagc ggcgtatatt aacagcacac aatccaatca agacattcag
     7921 agccgcttaa ctgctttgaa aaacggttct tatcatctct tttatattac gccggagcgt
     7981 ttaacctcac cggaattcat tcggatttta caaagcatca ccgtcccgct cgtggctatt
     8041 gatgaagccc actgcatttc gcaatggggg catgatttta ggccaagcta cagaaatatt
     8101 gaaattctat ttcgtgagtt aaaagagaag cctgtcatta tggcattaac cgcgacagct
     8161 actcctgagg tgcatgagga catttgcaaa cagcttcata ttgaaaagga aaatacggtt
     8221 tataccgggt tttcccgtga caatttaacc tttaaagtag caaaaggcga aaataaagat
     8281 cgctttatag aagagtacct gaacagaaat aagcttgaag caggcatcat ttatactgcg
     8341 acaagaaaag aagcagaccg gatctgtgaa aaactaaaac ggcagcatat aaacgcagga
     8401 cgttatcatg gtggattgcc ggatgaagtc aggaaagagc agcaggagca gtttcttaat
     8461 gaccaaatcc aagtgatggt cgcaacgtct gcttttggta tgggcatcga caaatccaat
     8521 atcagatttg ttttgcatta tcaaattcca aaagatatgg aaagctacta tcaagaggcg
     8581 ggaagggcag gccgtgacgg gcttgacagt gaatgtatcc ttctgttttc accgcaggat
     8641 attatggtgc agcgcttttt aatcgaacaa tcggcatcag atgaaggaaa gcaaaagcag
     8701 gatctgaaga aactgaggca aatggttgat tattgccaca cagaagattg cttgcagcga
     8761 tttattctta aatatttcgg tgaggttgat ccggcagcct gcggacaatg cggcaactgt
     8821 acagatacaa ggcaagcgca tgacgtcacc aaagaagcgc aaatggtatt atcgtgtatg
     8881 atacgtatga atgaacgatt cggcaaaacc ctgatcgctc aggtgctggc gggctcaaaa
     8941 aacaaaaaag tgctggataa cgggttcagc cggctatcga cgtacgggat tttaaagcat
     9001 caatctgtat ctgaaatcag cgattttatc gagtttctga tttctgatga ctttattcaa
     9061 atgtcagacg gtacgtttcc gactcttttt gtaaccaata aaggaagaaa cgtgctgctt
     9121 ggaaaagagg acgttgtcag aaaagaagcg ctcaaagcgg ctgcgattac tgaaaatgac
     9181 gccttgtttg agcggcttcg catgcttcga aagcaaattg ctgcagaaca aggcgtcccg
     9241 ccatttgtcg ttttttctga tcaaacgcta aaagaaatgt caggcaagca gcctgcgaat
     9301 gaagaggatc ttctttcaat taaaggagtg ggagaacaaa aaagggtgaa atacggcaag
     9361 ctttttttag aagaaattca agcttacgta agtgaaacag tataaaaaag caaaaaaaat
     9421 aagttttttt tggagaaaac acattttgtc cggagcggaa aaagccatgc aaataagact
     9481 tgtaaaaccg ctttatacca agggttcagc aagtctttcc aaccaagaga aatctttcat
     9541 gtaaaggaac tgtaatctaa ttcaaatcgt tttgttattg aaatgacatt tacatatgtt
     9601 acgatatccc ctgttagccg ccaataaaaa gctaacaagg gaggatttac ttatgaagaa
     9661 gacgattatg tcctttgtcg cagttgcagc aatctcatca actgcattcg gagctcacgc
     9721 ttctgcgaaa gaaatcacgg tgcaaaaggg tgacacgctc tggggaatct ctcagaaaaa
     9781 cggagtgaac ctaaaggact taaaagaatg gaatcagcta tctactgata taatcattgc
     9841 aggagagaaa ctgaccattt cttcagaaga agaaacaacc gctgggcaat acacagttaa
     9901 agagggggac agcctctgga agattgctca aaaatacggc atttcaatca acagccttaa
     9961 aagctggaac aatctagact ctgacatgat ctatgcagga acaacacttg cggttaaagg
    10021 tcaggtatca gcttcaaaca cagctcaaga gagccagcct caagctgagc caaaacaaga
    10081 agcggtgcaa acagagcagc caaaacaaga aactgttcaa caacaaccta aacaagaaac
    10141 tgttcatcaa caacctaaac aagaaactgt ccaacaacaa cctaaacaag aatcgaaggc
    10201 tgcagcagaa acttctgaga aaacagagga acagcctgtt caatcaaata caagcaatca
    10261 aaatgctaaa aaagaactaa cagttactgc aactgcttat tcagctaacg acggcggcat
    10321 ttccggcgtt acggccacag gtgtagactt gaataaaaac cctaacgcaa aagtaattgc
    10381 ggtggatcct aaagtgattc cgcttggttc aaaagtctac gttgaaggtt acggagaagc
    10441 aactgcagca gatactggcg gcgcaataaa aggaaataaa atagatgtat ttgtacctaa
    10501 aacaagtgac gcacataact ggggcgtcag aactgtaaag gtaaaagttc ttaactagtc
    10561 atatatcggt atttaaatca aggacactga gcgtaagctc gtgtcctttt ttttcttccc
    10621 tttttttctc tttcctaacc attatattct gaaaagttcg aatgtgcttc tcatgttttt
    10681 tctgttcgta aacagggaat aagggtctta tgttttgaaa aaaaggggat gcagaaattt
    10741 gttatggatt atcgtatctt ttttactcat taatatcggc atatttttgt tctcgtggct
    10801 aagaacgaag gatgtgaact tacatacgtc tgacggctat tttctcggag ggagaagcct
    10861 tactgcgttg tatataggga gttccttatt gcttaccaat ctatcgacag aacaaatggt
    10921 tggattaaac ggtcaaagct acgccggttc gatgactgca atgtcttggg aggcgagtgc
    10981 gccgattgct ttgatatttt tcgcgttact ttttcttccg cgatatttaa aaacaggcat
    11041 aacgacaata cccgattttc ttgaaaagcg ctttgatcaa aaaacaagac aaattgtctc
    11101 tttgttgctg atcattggct atgcggtttc attccttccg actgttttat attccggagc
    11161 acttgtatta gatcaggttt tcggttttac ggataatttt ggaatcagca cttttacagc
    11221 cgtattgatc atttcattta tcattggcat aacgagctgc tgttatatct tcttcggcgg
    11281 tttgaaagct gcggcctcag cagatgccat ctatggaatc ggtttgatct gcggcggctt
    11341 gctgattgtt gtgctcggta tattagctat cggtgaagga aactttggag gcggtgttgc
    11401 caaattaacg gccgtacata cagagaagct taattcaatc gatatcagca actccgcaga
    11461 agtgccatgg ccaaccattt taacgggcct cttagtaaat aatttgtttt actgggctgt
    11521 caaccaggcg atcgttcagc gcgcattagg cgctaaaaat ttggctgagg ggcaaaaagg
    11581 ggcgctctta gcaggatttt ttaaattagt cggggttttt tatttagtcc tgccgggtat
    11641 tattgctttt catctttatg gaggcggaat caataacgcc gacagtgtct atccccgcct
    11701 gatcgtagat ttacttccga cagcgcttac cggtattttt gcagctattt tattcggcgc
    11761 tattttaagc tcgtttaacg gttcattgaa tagtacgatt acattattta cacttgatat
    11821 ttataagccg atttttaaac cgaatgcaaa tgatgctgat cttgtaaaga caggcagaat
    11881 atttgtagca attcttggcg ttgtctccat tatcattgcg ccatttattt tgtatgcgcc
    11941 atcaggcttg tataattatc ttcaagaaat gtttggcttt tttaatgtgc cgatcttagc
    12001 tgcagttgtg gtggggttct tttctaagag agtacccgcc ttagccgtta aaattgcgat
    12061 accggctcat attgtcctgt atggcttatc taaaattttt ttggggcaca ttcattttct
    12121 gtatatccta gctgtcctct ttccgttctc ggtggcggtg atgctgtttg tcggaaggct
    12181 tcggcccaga gaaaaagagt atcgtcttaa ccatgatcct caagtggact tgaaaccttg
    12241 gaagcatgta aaacttgcgt catgtattat tctcgtgatg gtgtctgtca tttatattgt
    12301 cttttctccg tgggggattg ccggttaaaa caaaattcta aatagaatgg ggagagatgg
    12361 agctgaaccg cgaagtaaat agaaaagggg ttaaccctct aagacggaat gcttttgctt
    12421 ataattttaa agggctgaaa aacaagccgt tttgtttgca aaaagagcag gggaagcagt
    12481 catttgatcg gagcatgcgc ctgtgatata tgtttacttg cggacaactt ctgccagact
    12541 tatcttattg aatcagaata cagcccttat tttgatgtgc aggtgacact tgaagcaggg
    12601 gcctaaataa aaaggaaagg ctgattcaat aaaaaagagt atctcggaca tgaccaagat
    12661 gctctttttt tatttaaacc agcctttttc ctttgatcgg gtaatggctt caatccggtt
    12721 tttcacttca agcttatcga gtatcataga aatatagttg cggac
//
