LOCUS       NZ_JALAVQ010000007     10387 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus atrophaeus strain TP10O4N-G7 contig_0000007, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAVQ010000007
VERSION     NZ_JALAVQ010000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus atrophaeus
  ORGANISM  Bacillus atrophaeus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 441126)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 441126)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 345.71764523836856x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:42:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,242
            CDSs (total)                      :: 4,158
            Genes (coding)                    :: 3,989
            CDSs (with protein)               :: 3,989
            Genes (RNA)                       :: 84
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 74
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 92 of 169
            Pseudo Genes (incomplete)         :: 113 of 169
            Pseudo Genes (internal stop)      :: 22 of 169
            Pseudo Genes (multiple problems)  :: 50 of 169
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:20:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 245374
            Orig. end                         :: 255761
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAVQ010000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10387
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10387
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10387
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5387](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5387)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            225..1109
                     /locus_tag="MOE59_RS12995"
                     /old_locus_tag="MOE59_12970"
     CDS             225..1109
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388207.1"
                     /locus_tag="MOE59_RS12995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_12970"
                     /product="permease"
                     /protein_id="WP_010787583.1"
                     /transl_table=11
                     /translation="MTTQSSFLQLNSIFISILIEAIPFILIGVILSGLIQMFVSEEMIA
                     KIIPKNRFLAVLFGALTGILFPACECGIVPITRRLLLKGVPLHAGIAFMLTAPIINPIV
                     LFSTYIAFGNKWSVVFYRGGLALVVSIAVGLILSYQFKDNQLLEPSEPAHQHHHTQSLL
                     QKLGGTMRHAIDEFFSVGKYLIIGAFIAAAMQTYVKTSTLLSIGQNDVSSSLVMMGLSF
                     VLSLCSEVDAFIASSFSSTFSLGSLIAFLVFGAMVDIKNLLMMLAAFKKRFVFLLITYI
                     VVIVLAGSLLVKG"
     gene            1114..1968
                     /locus_tag="MOE59_RS13000"
                     /old_locus_tag="MOE59_12975"
     CDS             1114..1968
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388206.1"
                     /locus_tag="MOE59_RS13000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_12975"
                     /product="TIGR03943 family protein"
                     /protein_id="WP_268488073.1"
                     /transl_table=11
                     /translation="MFRLLVLMGFTFFFYHLHASGNLTKYINMKYSYLSFIAIFLLGIL
                     TVVQAYLYIKTPEKGKHHHHDQNCGCGHDHDHEQHKPFYKRYFIYLVFLFPLVSGIFFP
                     IATLDSTIVKAKGFSFKAMESGDHYSQTQYLRPDSSLYYADDSYDKLMKKLYKKYSGQK
                     HISLTDEDFLKGMETIYNYPGEFLGRTIEFNGFAYKGNAINKKQLFVLRFGIIHCIADS
                     GVYGMLVEFPKDMDIKDDEWIHIKGTLSSEYYQPFKSTLPVVKVTDWNTIKKPDDPYVY
                     RGF"
     gene            complement(2063..2707)
                     /locus_tag="MOE59_RS13005"
                     /old_locus_tag="MOE59_12980"
     CDS             complement(2063..2707)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328050.1"
                     /locus_tag="MOE59_RS13005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_12980"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_003328050.1"
                     /transl_table=11
                     /translation="MSYVMDLENIRKSYGEHVVLDKVNIVIEKGEMVAITGSSGSGKTT
                     ILNIMGILEAPDEGTVKILGDVAPRIGSRRANELLRSKIAYLFQNYALIDNASISANLD
                     VPLIYKKLKKEEKEKLKIEVLEKVGLKKVSLTQKVHELSGGEQQRLAIARILLKPCDII
                     LADEPTGSLDISNRNEVINLLKELNQMDKTIIIVTHDPYVSNQCSRVIELN"
     gene            complement(2712..4919)
                     /locus_tag="MOE59_RS13010"
                     /old_locus_tag="MOE59_12985"
     CDS             complement(2712..4919)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328049.1"
                     /locus_tag="MOE59_RS13010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_12985"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_268488074.1"
                     /transl_table=11
                     /translation="MKKIVVIFLVISSLFSFLISFRETSNKQIEEFEQLDSSIGQPFTI
                     PDDPLLNDPKEMIELIMSSSKKYNANIFRPAINFSTNDKVEISKYILLTQRTDYFNDIE
                     LLNGRFLSNLDTESKNFYISSQKSKNKNQIGQLEIFGSTSKITIKPLKQSFDYLPVSGT
                     YYVETTGTYSFKDFLKGFVNEINKKFDSSFEVSDFLQSGEVNDSYSGVSTSSLEQYNYL
                     VLLVTLFLLIYYIVNESKTIGVLKLNGYSNLRIWFQIVGKLLILTFCFSLLLSILCTIF
                     LPESTFSFFMNTVMKHIINYLIIGILSIFTYFYIRKIKVDLAIKNKKDTNSIFIFNSIL
                     KIICSILIIVIGSSTLNQYNEVKNKQSHLEDWNVSKDYGVFNPLYIGHDIEDVNNGKPQ
                     LTNNIDDIYPVLNKMGALLIDTREYEEEFVRLNSHYKGVRSIIVNNNYLKQFPVYSSEK
                     KPISINEREKRLILLVPNKYKQHREKIINFFKEDRKESAVAYDEEFLGRHVPKYLKEPE
                     IHIIWTASGQKIFSFNPEVFKAEDNLIVDPIIQVVTENNSLAGDRNTILGNGSRDPLKI
                     KLTNRDSKKTLYILEPYLKKYHLDDNLKYVVSLNQYALDEINELQSEISSLLAIAAVLI
                     VALVLLIVQNSVIYFNKKQQKFVVYRLFGIGYFRTFKDYMILFFLFWLIQFISCIVVKG
                     GISSNLLICTVFLLILELVISIIALNIIERRNKIKVMKGGF"
     gene            complement(5001..5387)
                     /locus_tag="MOE59_RS13015"
                     /old_locus_tag="MOE59_12990"
     CDS             complement(5001..5387)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328048.1"
                     /locus_tag="MOE59_RS13015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_12990"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_268488075.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.3e-07,
                     bitscore: 26.0, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKKGIMSAALIAALALSTGQAASAAGAATSGEVDLTNTPVKNSE
                     VTPFANEDVGGGNWDYGTSGYFDWGVKKKVWSNYYHKNKAHASSCALGTRTNSSGKIKA
                     GTTSYSSLSGGSKDAKTKANWKTY"
     gene            complement(5620..6849)
                     /locus_tag="MOE59_RS13020"
                     /old_locus_tag="MOE59_12995"
     CDS             complement(5620..6849)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328047.1"
                     /locus_tag="MOE59_RS13020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_12995"
                     /product="PucR family transcriptional regulator"
                     /protein_id="WP_268488076.1"
                     /transl_table=11
                     /translation="MEELLERVFSFSDIDKLIDFISYELQKPVILESADFFLLAYNSYY
                     INHFDSANQQTIFSKKCPVQIFERFLKDGIIEKLKTVPEPFRVTKIESIGLNQRVVVSA
                     KHKGEVMGYIWIQELDCNLTEEELAFLYETSFHVGKIIYKTKKLKQEKEERSEDLVKRA
                     IYQQFSSEKELRREAEKINTVLPSVFSVVILHAANGDGEAVEDLKENIRSYLNLRDKVS
                     HVLTIESNIVIVVASFSQKSSVSSAAAEFINKLLTHFHFQKIPTPIYIGIGNVYDNILN
                     LGKSYIEALEVIKAAEITGNQENIPYEYAKLGIYRYLESIDEKNQRLGYVNKDLALIKA
                     KDRESSTELLKTLETYLLNNCKTKPAAEQLFIHQNTLNYRIKQILEMTSIDLSDFRTRC
                     QLYLDLMLMK"
     gene            complement(7005..8459)
                     /gene="putP"
                     /locus_tag="MOE59_RS13025"
                     /old_locus_tag="MOE59_13000"
     CDS             complement(7005..8459)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /gene="putP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015239118.1"
                     /locus_tag="MOE59_RS13025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_13000"
                     /product="sodium/proline symporter PutP"
                     /protein_id="WP_073991413.1"
                     /transl_table=11
                     /translation="MIISICIYMVGMLFIGYFAYKRTSNLTDYMLGGRSLGPAVTALSA
                     GAADMSGWLLMGLPGAMFSTGLSGAWIVFGLVLGAWANWLFVAPRLRTYTEKAGNSITI
                     PGFLENRFGDHTKLLRLFSGLVILIFFTFYVSSGMVSGGVLFNSILGLDYHTGLWVVTG
                     VVVAYTLFGGFLAVSWTDFVQGIIMFAALILVPIVTLFHTGGVGETTAEIRSVNPELFN
                     IFKGTSVLSIISLFAWGLGYFGQPHIIVRFMAITSVKEIKRARRIGMGWMILSAIGALL
                     TGLAGIAYYNQRGLTLKDPETIFIQLGDILFHPIITGFLISAILAAIMSTISSQLLVTS
                     SSLVEDLYKAVFRRSASDKELVLLGRLSVLAVSIVALLLSWEKNNTILGLVSYAWAGFG
                     SAFGPVILLSLFWKRMTKWGALAGMIVGAVTVIIWANAGLSDFLYEMIPGFAASLLSVF
                     VVSLLTQAPSKAVAEQFKDYKDTLSQ"
     gene            complement(8551..10098)
                     /gene="pruA"
                     /locus_tag="MOE59_RS13030"
                     /old_locus_tag="MOE59_13005"
     CDS             complement(8551..10098)
                     /EC_number="1.2.1.88"
                     /GO_function="GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA]"
                     /GO_process="GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pruA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388203.2"
                     /locus_tag="MOE59_RS13030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE59_13005"
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="WP_010787580.1"
                     /sec_met_domain="Aldedh (E-value: 1.6e-154, bitscore:
                     509.5, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPYKHEPFTNFQDQKNIEEFQKALAVVNEYLGKDYPLVINGEK
                     VETEAKIVSINPANKEEVVGRVSKASQEHAEQAIEAAAKAFEEWRYTTPEERAAVLFRA
                     AAKVRRRKHEFSALLVKEAGKPWNEADADTAEAIDFMEYYARQMVELAKGKPVNSREGE
                     KNQYVYTPTGVAVVIPPWNFLFAIMAGTTVAPIVTGNTVVLKPASATPVIAAKFVEVLE
                     ESGLPKGVVNFVPGSGAEVGDYLVDHPKTSLITFTGSREVGTRIFERAAKVQPGQQHLK
                     RVIAEMGGKDTVVVDEDADVELAAQSIFTSAFGFAGQKCSAGSRAVVHEKVYDQVLKRV
                     IEITESKVTASPDSADVYMGPVIDQGSYDKIMSYIEIGKEEGRLVSGGTGDDSKGYFIK
                     PTIFADLDPNARLMQEEIFGPVVGFSKVSNFDEALEVANNTEYGLTGAVITNNRKHIER
                     AKQEFHVGNLYFNRNCTGAIVGYHPFGGFKMSGTDSKAGGPDYLALHMQAKTISEMF"
ORIGIN
        1 cccataaact cattccgcaa tctgaactct gtttgcttca gtcatggggt catctcattt
       61 ggcttggaaa agaatccgat gaagttgatc acacattagc cactttcctg agctcatcat
      121 catgacgaac atgtgaaacg attctctgct tttttccgtc aaacaataag tgcctttctt
      181 catgatataa tcataggcag cttttaaaaa agcggaggtt ttatatgacg acacaatctt
      241 cttttcttca gctaaattct atttttatca gtatattaat agaagccatt ccgtttattc
      301 tgattggcgt cattctttcc ggactcattc aaatgtttgt ttcagaagaa atgattgcaa
      361 aaattattcc gaagaaccgg ttcctggctg tgctattcgg ggcgttgacc ggaatattgt
      421 tcccggcctg cgaatgcggc atcgtgccga tcacgagacg gcttcttctc aaaggggtgc
      481 cgctccacgc gggcatcgct tttatgctga ccgcgcctat catcaatccg attgttttgt
      541 tctctacata cattgcgttc ggaaacaaat ggagcgttgt cttctaccgg ggcgggcttg
      601 cacttgtcgt gtctattgct gtcgggctga ttttatcgta ccagttcaag gacaaccagc
      661 tgcttgagcc ttccgaaccc gcacatcagc atcatcatac acaaagtttg ctgcaaaaat
      721 tgggcggaac gatgcggcat gccattgatg agtttttttc agtgggaaaa tatttgatta
      781 tcggcgcctt catcgcggcc gccatgcaga cgtatgtcaa gacatccact ctgctttcaa
      841 tcgggcaaaa tgatgtgtcc tcttcccttg tgatgatggg tctgtcgttt gtcttatctc
      901 tctgttcaga agtagatgcc tttatcgcat cttccttcag cagcacattt tcattaggat
      961 cattaatcgc ctttcttgtt tttggtgcga tggttgatat taaaaatctg ttaatgatgc
     1021 ttgcggcttt taaaaaacga tttgttttcc tgctcattac gtatattgtc gtgattgtgc
     1081 tagcgggatc attacttgtg aaggggtgat cggatgtttc gtctattagt gctgatggga
     1141 tttacctttt tcttttatca tctgcacgcc tcaggaaatc tgacaaaata tattaatatg
     1201 aaatattcct acctgtcctt tatcgcgatc ttcctgctgg gcatcctgac agtcgttcag
     1261 gcgtatcttt atatcaaaac gccggaaaaa ggaaaacacc atcatcatga tcaaaactgc
     1321 ggctgcggcc acgatcatga tcatgaacaa cacaagccat tttacaaacg ctattttatt
     1381 tatcttgttt ttctttttcc tttggtttca ggcattttct tcccgatcgc cacgctcgat
     1441 tcaacgattg taaaggcaaa aggcttttca ttcaaagcca tggaatccgg cgatcactac
     1501 tcgcaaaccc aatatttgcg gccggactcc agtctttact atgctgatga cagctacgac
     1561 aagctcatga aaaagctgta taagaaatac tcgggccaga aacatatttc tctgaccgat
     1621 gaggatttct tgaaagggat ggaaaccatc tacaattatc cgggtgaatt cctcggacgc
     1681 acgattgagt ttaacggatt tgcttataag gggaatgcca ttaacaagaa gcagctcttt
     1741 gtgctgagat tcggcatcat tcactgtatc gcagattccg gcgtatatgg gatgcttgtg
     1801 gagttcccta aggacatgga tattaaagat gacgagtgga ttcatattaa gggcacactg
     1861 tcctcagaat attaccagcc attcaaatca acgcttcccg tcgtgaaagt gacagactgg
     1921 aacaccatta aaaaacctga tgatccgtac gtttacagag gattttaaac aggcttgagg
     1981 taccaaaaaa agttggttcc tcaagcctgc ttacatattt tattttgaac gtacccatat
     2041 acatattaaa gagtttcatt ttttagttta gttcgataac tcgactacat tgatttgata
     2101 cataaggatc atgagttact attattatag ttttatccat ctgatttaat tcttttaaaa
     2161 ggttaataac ttcattacga ttacttatat ctaaagatcc tgtcggttca tctgcaagaa
     2221 taatatcaca tggttttaat aaaattcttg ctattgctaa tctctgttgt tcgcccccag
     2281 aaagctcatg gactttttgt gttaaggaca ctttttttaa acccactttt tcgagaacct
     2341 caatcttcaa tttttccttt tcttctttct ttaacttttt ataaattaat ggaacatcta
     2401 aattcgcact gatactagca ttatcaatca aggcataatt ttgaaataaa tatgctattt
     2461 tagacctcaa caattcattt gctctccgag aaccaattct cggagcaaca tctccaagaa
     2521 tttttactgt tccttcatca ggagcttcga gtatgcccat gatattcagt atcgttgttt
     2581 ttcctgaacc gctactgcct gtaattgcga ccatttctcc tttttctata acaatgttga
     2641 ctttatccaa tacaacatgt tccccataag attttcttat attttctaga tccattacat
     2701 aactcaatta atcaaaaacc tcctttcata acttttatct tgtttcttcg ctctataata
     2761 tttaatgcaa taatcgaaat aaccaattct aaaattagaa gaaatacagt acaaattaat
     2821 aagtttgaac ttattccccc tttgactaca atacatgata taaattgtat gagccaaaat
     2881 aagaaaaaca agatcatata atctttaaat gtcctaaaat aaccaatgcc aaacaatcta
     2941 tagactacaa atttttgttg ctttttatta aaatatatta cagaattttg cacaataaga
     3001 agaaccagag cgacaattaa tacagcagct atagctaaaa gtgagctaat ttcgctttgt
     3061 aactcgttga tttcatccaa agcatattga ttcaaagaga ccacatactt caaattgtca
     3121 tcaagatgat atttttttaa ataaggttct aatatataca atgtcttttt tgaatctcta
     3181 ttagttaact ttattttaag cggatcccga cttccatttc ctaaaattgt attcctgtca
     3241 cctgccaagc tattattctc tgtaaccact tgtataatag gatctacaat taaattatcc
     3301 tcggctttaa agacttctgg attaaaacta aaaattttct gaccactagc cgtccaaata
     3361 atatgtattt ctggttcttt taaatacttc ggtacatgtc tacctaaaaa ttcttcatca
     3421 taagctactg cagattcttt tctatcttct ttaaaaaaat ttattatttt ttctctgtgt
     3481 tgtttatatt tattcgggac aagtaatatt aatcttttct ctctctcatt aattgaaatt
     3541 ggttttttct ctgagctgta tacaggaaat tgttttagat aattattatt aacaatgata
     3601 gatctaactc ccttatagtg ggagtttaat cgaacaaatt cttcttcata ctctcttgta
     3661 tctattaaga gagcccccat tttgttcaaa acaggataaa tatcatctat gttattcgta
     3721 agttgcggct ttccattatt tacatcctca atatcatgac ctatatacaa agggttgaaa
     3781 accccataat ccttactgac gttccaatct tctaaatgag attgtttatt tttcacttcg
     3841 ttatattgat ttaaagttga actacctatc acaattatta agatcgaaca aatgattttt
     3901 aaaatagaat taaagataaa gatgctattt gtatctttct tattctttat tgctagatca
     3961 acctttattt ttcttatata aaagtaagta aaaattgata atatacctat aatcaaataa
     4021 ttaataatat gtttcataac agtattcata aaaaaagaaa aggttgattc aggtaagaaa
     4081 attgtacata ggattgataa taacaatgaa aaacaaaacg ttaatattaa taatttcccc
     4141 actatttgaa accaaattct taaattagaa tacccattca gctttaaaac acctattgtt
     4201 tttgactcat tcactatata ataaataaga agaaataatg ttacaagaag aaccaaatag
     4261 ttatactgtt ctaatgatga cgtagagact cccgagtagc tatcattaac ttctcctgat
     4321 tgtaaaaagt cacttacttc aaacgacgaa tcaaattttt tatttatttc attaacaaat
     4381 ccttttaaaa agtctttaaa agaatacgta cctgtagttt caacataata tgtaccgctt
     4441 acaggaagat aatcgaatga ttgttttagt ggtttaattg ttattttaga tgtgctgcca
     4501 aatatttcaa gctgtcctat ttgattttta ttcttagatt tttgcgaact tatataaaag
     4561 tttttagact cggtatctaa attacttaaa aatctcccat ttaagagttc aatatcatta
     4621 aaatagtcag ttcgttgcgt taataatata tacttagaaa tttccacttt atcgtttgta
     4681 gagaaattaa tagccggccg aaaaatatta gcattatatt ttttggaact actcataatt
     4741 agttcgatca tttctttagg atcatttaac aagggatcat caggtatagt aaaaggttgt
     4801 ccaatagaac tatctaactg ttcaaactct tcaatttgtt tattcgaagt ttccctaaag
     4861 gaaattaaaa aggaaaacag tgaagatatc acaagaaata taaccacaat ttttttcaca
     4921 caaaaaaact cctttataaa aaggagaatc atcgaaaact atctctaagt tttcgatgat
     4981 atccttatta taattttata ttagtatgtt ttccaattgg cctttgtctt agcatctttt
     5041 gatccgcctg acaaactgga gtatgaggtt gttcctgctt taattttacc gcttgaatta
     5101 gttcttgttc ccagagcaca ggatgaagcg tgggccttat ttttatgata atagtttgac
     5161 caaactttct ttttaactcc ccaatcaaaa taaccagatg taccataatc ccagtttcct
     5221 ccaccaacat cttcatttgc aaatggagtt acttcagagt ttttcacagg tgtattagtt
     5281 aaatcaacct cgccacttgt tgctgcacct gctgctgatg ctgcttgtcc agttgacaaa
     5341 gctaatgctg ctatcaaagc tgcactcatg ataccttttt ttaccactgt taatcctctc
     5401 cttctttttt aagatagatt gaatgacagc gagctcatct gtttcaacca caaccttatt
     5461 ataccatatt tacaaaatat agcaaatagt catttttccc tatatatggg taattaaact
     5521 aaatacttaa atacttatac atataaaaaa acaacaaatt tcacatttcg agttttaata
     5581 tatctcttat aacaattagc caaaataaaa tagtatgtat tatttcatca gcatcaaatc
     5641 cagataaagc tggcatcgtg ttctgaaatc acttaaatca atcgatgtca tctcaagaat
     5701 ctgcttaatg cggtagttta atgtattttg atgaataaac agctgttctg cggccggttt
     5761 tgttttgcaa ttattcaaga gataggtttc cagtgttttc agcagctctg tactgctttc
     5821 cctgtccttc gcctttatta atgccaagtc tttattgaca tatccgagac gctgattctt
     5881 ttcatctatg ctttcaagat agcggtaaat gccgagttta gcatattcat acggaatgtt
     5941 ctcctgatta cccgtgattt ccgccgcttt aatgacttcc agtgcttcta tgtaactctt
     6001 gccaaggttc aaaatgttat cataaacgtt accgataccg atataaattg gggtaggaat
     6061 tttttgaaag tgaaaatgag ttaacagctt gttaataaat tcagcagctg ctgaggagac
     6121 ggagcttttt tgggaaaaac tcgctacgac aattacaata ttcgattcaa tggttagcac
     6181 atggctgacc ttatcccgta aattgaggta tgatctgatg ttttctttta aatcctccac
     6241 ggcttctcca tccccattgg cagcgtgtaa gatcacgaca gaaaacacgg aaggcagcac
     6301 ggtgttgatt ttctcagctt cccgcctgag ttccttttca gatgaaaatt gctgataaat
     6361 agcccttttc accaagtctt ccgatctttc ttctttttcc tgtttcagct tttttgtttt
     6421 atatatgatt ttccccacat gaaaggaagt ctcgtacaaa aacgccagtt cttcctctgt
     6481 aagattgcaa tcaagctctt gaatccagat gtagcccatg acttcccctt tgtgcttagc
     6541 actcacgacc accctctggt ttaacccgat gctctctatt tttgtgaccc gaaatggttc
     6601 aggaaccgtt tttaactttt ctatgattcc gtcttttaaa aatctctcga atatttgcac
     6661 cggacacttc ttcgagaaaa tcgtctgctg gttcgcggaa tcaaaatggt taatgtaata
     6721 agaattgtag gcaagcaaaa agaaatccgc gctttccaga atcaccggtt tttgtaattc
     6781 atagctgata aaatcaatta atttatcaat gtctgaaaat gaaaaaactc tctctaaaag
     6841 ctcttccata ccttttttct cctatgcttt tttactttaa tcatactttg tacaagggct
     6901 ttttgtatag gaaaatataa gattgtttcg taaatgctct cctcaaggaa aaaagagaaa
     6961 gaggaagaaa gatgattccc ttcctcctct tttctatatc ccctttactg tgacagcgta
     7021 tctttatagt ccttaaactg ctctgcgact gcttttgacg gagcctgtgt caataagctg
     7081 acaacaaaga cagatagcag actcgcggca aagccgggaa tcatttcata cagaaagtcc
     7141 gaaagaccgg cattcgccca aatgattaca gtaactgccc ccacaatcat gccggcgagt
     7201 gccccccatt tggtcatccg tttccaaaac aaactgagca agataactgg cccgaatgct
     7261 gaaccaaagc cggcccaggc ataactgacc aagcccaaga tcgtgttgtt tttctcccat
     7321 gacaatagta aagcgacgat tgaaacagca agcacagaca gccggcctaa taacaccagc
     7381 tctttatctg aagctgaacg tctgaatacc gccttataca aatcttccac cagagagctt
     7441 gatgttacaa gcagctgtga agaaatcgtg ctcataatgg ctgctaagat ggctgaaatc
     7501 aaaaatccgg taataatcgg gtgaaacaaa atatctccca attggatgaa gattgtttcc
     7561 gggtccttca atgtaaggcc ccgctgattg taatacgcga ttcccgccaa accagtcagc
     7621 aatgcgccga tagccgaaag aatcatccat cccattccga ttcttcgggc ccgtttgatc
     7681 tcttttactg acgtaatcgc cataaaacgc acaataatat gaggctggcc aaagtaaccc
     7741 agaccccaag caaacaaaga aataatacta agcacgctgg ttcctttgaa aatattaaac
     7801 aattccggat taactgaacg gatttcggca gttgtttctc ctactccgcc ggtatgaaac
     7861 aatgtgacaa tcggtacaag tataagagcc gcaaacataa taatcccctg aacgaagtca
     7921 gtccagctga ccgcaagaaa ccctccaaac agtgtataag cgacgacgac acctgtcaca
     7981 acccataacc ccgtatgata atccaaacct aaaatactgt taaacaatac accgcctgac
     8041 accattccag acgaaacata aaaggtgaaa aagattaaga tgacgagtcc cgaaaacagt
     8101 ctgagaagct tggtatgatc accaaagcga ttttcaagaa atcccggaat tgtgatggaa
     8161 ttccccgcct tttcggtata cgttcttaat cgaggcgcca caaacagcca gtttgcccat
     8221 gcacctaaaa cgagtccgaa cacaatccat gcgccgctga ggccggttga aaacattgcg
     8281 cccggcagcc ccattaagag ccagccgctc atgtcagccg ctcccgcact gagagccgta
     8341 acggccggac ctaatgacct tcctccaagc atgtaatctg tcagatttga agttcgtttg
     8401 taagcaaagt agccgatgaa aagcatgccc accatataaa tacatattga aataatcagc
     8461 tgcgtgtttt ctatagcact tcctcctccg ctatgttgtg atatggatag aatggacatc
     8521 cccgcaggga ggatgcccat ttagtcccga ttaaaacatt tcactgattg tttttgcctg
     8581 catgtgcaga gccaagtaat ccggaccgcc ggcttttgaa tccgtaccgg acattttgaa
     8641 gccgccaaac ggatggtagc cgacaatcgc gcccgtacag ttacggttga agtataggtt
     8701 gcccacatgg aactcctgtt ttgcgcgttc aatatgtttg cggttgtttg tgatcaccgc
     8761 gcctgtcaga ccgtattccg tattgttcgc cacttctaaa gcttcatcaa aattggacac
     8821 tttagagaaa ccgacaacag gaccgaaaat ttcttcctgc atcaatcttg cattcggatc
     8881 aaggtcggcg aaaatggtcg gcttgatgaa gtagcctttt gaatcgtcac cagtgccgcc
     8941 gctcactaaa cggccttcct cttttccgat ctcgatataa ctcataattt tatcataaga
     9001 accttgatca atgacaggtc ccatataaac atctgcgctg tcagggcttg ctgtcacttt
     9061 tgattccgtg atttcaataa cgcgcttcaa gacttgatca tacacttttt catgaaccac
     9121 tgcacgtgaa cccgcggaac atttttggcc tgcgaatccg aacgctgaag taaagatcga
     9181 ctgggcagcc aattcaacgt ctgcatcctc atcgacaaca accgtatctt taccgcccat
     9241 ttcagcaatc acacgcttca ggtgctgctg gccaggctgt acttttgcgg cgcgctcaaa
     9301 aattcttgtg ccgacttctc ttgatcccgt gaacgtaata aggcttgttt tcggatgatc
     9361 gacaaggtag tcgcccactt cagcgccgct tcccggaaca aagttgacaa cgcctttcgg
     9421 aagacctgat tcttcaagca cttctacgaa cttagcagcg ataaccggtg ttgcgctagc
     9481 cggtttcaga acaaccgtat ttcctgtcac aatcggcgct acagttgtac ctgccatgat
     9541 cgcaaataag aagttccaag gcggaataac aaccgctaca cccgtcggcg tgtatacata
     9601 ttggttcttt tcaccttcgc ggctgtttac aggctttcct tttgccaatt caaccatctg
     9661 gcgcgcgtaa tattccataa agtcaatcgc ttctgccgta tcagcatccg cttcattcca
     9721 aggttttccc gcttctttca caagcaaagc cgagaattca tgctttctgc ggcggacttt
     9781 ggcagcagcg cggaataaaa cagccgctct ttcctcagga gttgtgtatc tccactcttc
     9841 gaacgctttc gcagcggctt cgattgcctg ttctgcatgt tcttgtgatg cttttgagac
     9901 tctgccgaca acttcttctt tattcgccgg gttgatagat acgatttttg cttctgtttc
     9961 cactttttct ccattgatga caagcggata atctttgcct aaatattcgt ttacaacggc
    10021 aagcgccttt tggaattcct caatattctt ctgatcttga aaattcgtga atggctcgtg
    10081 tttgtaaggt gttgtcatag taatctctcc ttctgtgttg gtgttatttc ttcgtcatac
    10141 ctttaaatgc gaatgcgatg tttgacgggc gttcggccag acgtctcata aagtagccgt
    10201 accaatcgtt tccatatggg aggtacactc tcatgttata tccttctttt acgaggcttt
    10261 gttgggtttg cgagcgcatg ccgtacagca tctgaaactc aaacatgtct ttcgtgatgt
    10321 tatgctcact ggcaagctgt ttcgtaaact caatcatttt gtcgtcatgc gacgcaattg
    10381 cagtata
//
