LOCUS       NZ_JALAVI010000019     12949 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain EC30O4B-A7 contig_0000019, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAVI010000019
VERSION     NZ_JALAVI010000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 136063)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 136063)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 168.96085678627867x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:36:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,244
            CDSs (total)                      :: 4,173
            Genes (coding)                    :: 4,038
            CDSs (with protein)               :: 4,038
            Genes (RNA)                       :: 71
            rRNAs                             :: 4, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (5S)
            tRNAs                             :: 60
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 135
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 135
            Pseudo Genes (frameshifted)       :: 86 of 135
            Pseudo Genes (incomplete)         :: 70 of 135
            Pseudo Genes (internal stop)      :: 30 of 135
            Pseudo Genes (multiple problems)  :: 40 of 135
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:28:30
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 123114
            Orig. end                         :: 136063
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAVI010000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12949
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..12949
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12949
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[10000:10747](-)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10747)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            complement(426..1358)
                     /gene="hprK"
                     /locus_tag="MOE80_RS19850"
                     /old_locus_tag="MOE80_19790"
     CDS             complement(426..1358)
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /GO_process="GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /gene="hprK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391380.1"
                     /locus_tag="MOE80_RS19850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19790"
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="WP_003235406.1"
                     /transl_table=11
                     /translation="MAKVRTKDVMEQFNLELISGEEGVNRPITMSDLSRPGIEIAGYFT
                     YYPRERVQLLGKTELSFFEQLPEEDKKQRMDSLCTDVTPAIILSRDMPIPQELIDASEK
                     NGVPVLRSPLKTTRLSSRLTNFLESRLAPTTAIHGVLVDIYGVGVLITGKSGVGKSETA
                     LELVKRGHRLVADDCVEIRQEDQDTLVGNAPELIEHLLEIRGLGIINVMTLFGAGAVRS
                     NKRITIVMNLELWEQGKQYDRLGLEEETMKIIDTEITKLTIPVRPGRNLAVIIEVAAMN
                     FRLKRMGLNAAEQFTNKLADVIEDGEQDE"
     misc_feature    1470..1485
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
     gene            1540..2730
                     /gene="nagA"
                     /locus_tag="MOE80_RS19855"
                     /old_locus_tag="MOE80_19795"
     CDS             1540..2730
                     /EC_number="3.5.1.25"
                     /GO_function="GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA]"
                     /GO_process="GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nagA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391381.1"
                     /locus_tag="MOE80_RS19855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19795"
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="WP_268399749.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 5.4e-43, bitscore:
                     140.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAESLLIKGIAIVTENEVIKNGYVGINGGKISTVSTERPKESYSK
                     EIQAPADSVLLPGMIDIHIHGGYGADTMDASFSALDTMSSRLPEEGTTSFLATTITQEH
                     GHISQALVSARDWKAAEESSLLGAELLGIHLEGPFVSPKRAGAQPKEWIRPSDVALFKK
                     WQQEAGGLIKIVTLAPEEDQHFELIRYLKDESIIASMGHTDADSALLSEAAKAGASHMT
                     HLYNAMSPFHHREPGVIGTALAHDGFVTELIADGIHSHPLAAKLAFLAKGSSKLILITD
                     SMRAKGLKDGVYEFGGQSVTVRGKTALLSDGTLAGSILKMNEGARHMREFTNCSWTDIA
                     NITSANAAKQLGIFDRKGSVTEGKDADLVIVSSDCEVILTICRGNIAFISKEADQI"
     gene            2727..3455
                     /gene="nagB"
                     /locus_tag="MOE80_RS19860"
                     /old_locus_tag="MOE80_19800"
     CDS             2727..3455
                     /EC_number="3.5.99.6"
                     /GO_function="GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA]"
                     /GO_process="GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="nagB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391382.1"
                     /locus_tag="MOE80_RS19860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19800"
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="WP_268399750.1"
                     /transl_table=11
                     /translation="MKVVECQTYEELSQKAARIAADTIKEKPDAVLGLATGGTPEGTYR
                     ELIRLHQTENLSFKNVTTVNLDEYAGLSSGDPNSYHYYMNSRFFQHIDSQPSRHFIPNG
                     NADDLEAECRRYEQLVESIGGTDIQLLGIGRNGHIGFNEPGTPFKSRTHVVTLNEQTRQ
                     ANARYFSSIDSVPKKALTMGIQTILSSKRILLLISGKSKAEAARKLLEGDVSEDFPASA
                     LHLHPDVTILIDREAAALRP"
     gene            3473..4204
                     /gene="nagR"
                     /gene_synonym="yvoA"
                     /locus_tag="MOE80_RS19865"
                     /old_locus_tag="MOE80_19805"
     CDS             3473..4204
                     /codon_start=1
                     /gene="nagR"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /gene_synonym="yvoA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391383.1"
                     /locus_tag="MOE80_RS19865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19805"
                     /product="N-acetylglucosamine utilization transcriptional
                     regulator NagR"
                     /protein_id="WP_268275947.1"
                     /transl_table=11
                     /translation="MNIDKQSPIPMYYQIMEQLKAQIKSGELQPDMPLPSEREYAEQFG
                     ISRMTVRQALSNLVNEGFLYRLKGRGTFVSKPKMEQALQGLTSFTEDMKSRGMTPGSRL
                     IDYQLIESTEELAALLGCGHPSPIHKITRVRLANDIPMAIESSHIPFELAGELNESHFQ
                     SSIYEHIEKYNSIPISRAKQELEPSAATSKEASILGIQKGAPVLLIKRTTYLQNGTAFE
                     HAKSVYRGDRYTFVHYMDRLS"
     gene            complement(4223..8092)
                     /locus_tag="MOE80_RS19870"
                     /old_locus_tag="MOE80_19810"
     CDS             complement(4223..8092)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391384.1"
                     /locus_tag="MOE80_RS19870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19810"
                     /product="metallophosphoesterase"
                     /protein_id="WP_268399753.1"
                     /transl_table=11
                     /translation="MRKYTVIASLFLSFLSVLSGGHHESKAFPVIKQEPFQTPHYIPLL
                     ENPPQIDVKAEMKSNLMIEAQVRDNEYQAFSAFLFYKQSNELGYKMVPMDPTPGAIHKF
                     LAQIPKRLIWNSELEYYIVISNGKQRVESETKKLKLEGYQADLAHIPELLITELAVDTK
                     NTGRADGYEFIEIYNTTDRTIDFKDYHIRYRYPKEGPDSDLIWRPDERITIPSGETFVV
                     WLKPVGHPELTAEDFNRYYQTQLTEGKNLAVLEETEGMANTRPRSVVISTNTGKDISVA
                     HYRKHALRRLSSVLYKYPLDGTAELLNISIGEKNPSPGTVLQAQVPEQKRKIKLDKEKP
                     VIEDLTDRKPVRPAESIELRADIRDRSLVKTAAFYYRTDENKPFKRILAEKDRNDNLYH
                     YIVYSPELIGKDQLEYYVAAGDGINEARTPVKMIDIKQTSKSHGLRMNIENRDTLSGTQ
                     FLKATTDGRTNSIKLWIDGKKQVTEPAMEKEVYFAFDTRKTNLYFKNAVTMEGKVLKVF
                     DDTTNKYRTYSVPLPELLLRKGKQLQRITIRSGSKVSPFDTAENRDDFLVKNARLVLSD
                     GTVIRDQRISPEKELFIGDNQRSNKSWQFQFNLPDGLFTSQLLKWDTSKLSEGAHHIQA
                     SDGKENVSLVVRVDNSGPHIEPNITEGQTYKGNLILQADMYDKWSKIEEAEASLDGESI
                     TLPYHTSSSELLPGKHSLKITATDLAGNKTIIERTFKTEREHPDQPEVIDSEADTHRAK
                     LSVRVKDPTDDPMDVAFYRGFQYTARNNVKIFKHASLTEPPKSFVPESETPFTNKELER
                     VSAADGKTVSTEKKELFPYHRFEVTVDPSVDENDLAEAVWKGSSLPGRKVTMYAWNYRT
                     NEWQPVDSFVAKDHKPFTLKASVIASDFVRKSKMNVIVQDEIPPAKDMYTFAWMSDTQY
                     YAESYPHIFDKQTAWIKSNQKQLNIKYVFHTGDIVDDSADIRQWKNADRSMSVLDKSGI
                     PYGVLAGNHDVGHKDGSYRAFGKYFGSHRFDKKLHYGGSYKNNRGHYDLISSNGNDYIM
                     LYMGWGITDEDIAWMNQVLKKHPDRMAVLAFHEYLLVSGNRSPIGEKIFKKVVKPNPNV
                     VMVLSGHYHSAMRKTDELDDDGDGKPDRLVHQMLADYQGGPEGGQGYLRLLQFDQANDM
                     VHVSTYSPHLQDKNYYDTDTYGNKDEFSLSLDLKPRVKKVETDYFECNVYTNEELGKRE
                     QVKSGDTAEFRWDDLEPHSVYYWYTIVEDSFNGKTKSPIWKFKTKKETYRPSPDQFDFR
                     HVSNP"
     gene            8257..8730
                     /locus_tag="MOE80_RS19875"
                     /old_locus_tag="MOE80_19815"
     CDS             8257..8730
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391385.1"
                     /locus_tag="MOE80_RS19875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19815"
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_003235411.1"
                     /transl_table=11
                     /translation="MDNSIHDELFQAIQQFALKRDKRVWQKVQIPSRGMSSAHLEHLKK
                     DWTLTQLHIVSCIHTSQNVNNSFLASRLHISKAAVSKAVQALLKHNIITVTKKPGNKKE
                     IFYTLTESGRELAALHEQLHEKAKEQYKQLFNEFSIDDLKTVTAFFNLWVKHM"
     gene            complement(8766..9986)
                     /gene="cypX"
                     /locus_tag="MOE80_RS19880"
                     /old_locus_tag="MOE80_19820"
     CDS             complement(8766..9986)
                     /EC_number="1.14.15.13"
                     /codon_start=1
                     /gene="cypX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235412.1"
                     /locus_tag="MOE80_RS19880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19820"
                     /product="pulcherriminic acid synthase"
                     /protein_id="WP_268399755.1"
                     /sec_met_domain="p450 (E-value: 1.3e-38, bitscore: 125.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQSIKLFSVLSDQFQENPYAYFSQLREEDPVHYEESIDSYFISR
                     YHDVRYILQHPDIFTTKSLVERAEPVMRGPVLAQMHGKEHSAKRRIVVRSFVGDALDHL
                     SPFIKQNAENLLDPYLEKGRIDLVNDFGKTFAVCVTMDMLGLDKRDHEKIAEWHSGVAD
                     FITSIFQTPEARAHSLWCSEQLSDYLMPVIEERRVNPGSDLISILCTSEYEGMAMSDKD
                     ILALILNVLLAATEPADKTLALMIYHLLNNPEQMNDVLADRSLVPRAIAETLRYKPPVQ
                     LIPRQLSQDTVVGGMEIKKDTIVFCMIGAANRDPEAFEHPDVFNIHREDLGIKSAFSGA
                     ARHLAFGSGIHNCVGAAFAKTEIEIVANIVLDKMRNIRLEEGFRYAESGLYTRGPVSLH
                     IAFDRAQ"
     gene            complement(10001..10747)
                     /gene="pchC"
                     /locus_tag="MOE80_RS19885"
                     /old_locus_tag="MOE80_19825"
     CDS             complement(10001..10747)
                     /EC_number="2.3.2.22"
                     /codon_start=1
                     /gene="pchC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391387.1"
                     /locus_tag="MOE80_RS19885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19825"
                     /product="cyclo(L-leucyl-L-leucyl) synthase"
                     /protein_id="WP_225514486.1"
                     /sec_met_domain="CDPS (E-value: 6.5e-83, bitscore: 270.7,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEMVTERKPVDFTAESLTQNCREIFERRRHVLVGISPFNSRFSE
                     DYIYRLIGWATAEFKSVSVLLAGHEAANLLEALGTPRGKAERKVRKEVSRNRRFAERAL
                     AAHGGDLKAIHTFSDFTENKAYQLMRQEVEHAFFEQPHFRHACLDMSREAMIGRARGIS
                     LMMEEVSEDMLNLAVEYVIAELPFFIGAPDILEVEETLLAYHRPWKLGEKISNHEFSIR
                     MRPNQGYLIVQETAQMLSETRITSEG"
     gene            11178..11687
                     /gene="pchR"
                     /locus_tag="MOE80_RS19890"
                     /old_locus_tag="MOE80_19830"
     CDS             11178..11687
                     /codon_start=1
                     /gene="pchR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391388.1"
                     /locus_tag="MOE80_RS19890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19830"
                     /product="pulcherriminic acid biosynthesis transcriptional
                     regulator PchR"
                     /protein_id="WP_100276382.1"
                     /transl_table=11
                     /translation="MSDLTKQMIYDIYVRLLHLNEQKANTSLQQFFKEAAEEDVIDIPK
                     NMTSIHVIDCIGQHEPINNAGIAKKMNLSKANVTKISTKLIKEEFINSYQLTDNKKEVY
                     FKLTRKGKRIFDLHEKLHKKKELAFYQFLDSFSQDEQNAVLKFLEQLTSTLEAEQTDGT
                     PDKPVK"
     gene            11709..12917
                     /locus_tag="MOE80_RS19895"
                     /old_locus_tag="MOE80_19835"
     CDS             11709..12917
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391389.1"
                     /locus_tag="MOE80_RS19895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE80_19835"
                     /product="MFS transporter"
                     /protein_id="WP_268396802.1"
                     /transl_table=11
                     /translation="MAQMKSKAVLILYIVCFSAFFASLSQNIYSPIIPIIKESFHVSAA
                     MVNLSVSVFMIVTAIMQIILGTIIDFKGARFVLIAGILATTAASIGCAVTTDFTLFLIF
                     RMIQAAGSAALPLIAATTIGQLFTGSERGSAMGTYQMLLSAAPAVAPVLGGFIGGAAGY
                     EGIFWMLTAISVVMLVANSMIFPKDSPAGSKQKTKGNVFTHYKSIFTNRTGNVILALSF
                     VLFFIYFAVIVYLPILLTEHYHIDVSIAGLLYLPLALSTIAGTFLFKRIQAKISLNTLF
                     IGSNAVAACSIMLFAVTHSVSLVLMALTLALFGISMGVIPPLYSTMITNEFEHNRGSAI
                     GIFNFIRYTGMAAGPMISAYLLTMMPSAMSFGLLGLLFAALGFCLMPQMYPPQKRVKKK
                     HHM"
ORIGIN
        1 agcggccaat cgcctggccg agcaaaatgc ttggcgcggc aatatccgca agcttccaaa
       61 acgaaaggtt tttcactctt gaaaacacat aacctgttaa aatggcgccg attaacccgc
      121 cgtgaatggc aatgccgccc ttccaaatct taatgatttc tcccggatgc gctgcataat
      181 aatcccattc aaatgcaacg taataaattc gtgcgcaaat aatcgcaatc ggaatggcaa
      241 acaggacaag atcgataaac gtatctttct gcagccccct tttctcactt tccctcatgg
      301 caatccaaag cccgagcaaa gcgccgagac cgataatgat tccgtaccaa tggacggcta
      361 aaggccccag ctgaaaagct atcggattga gtggttctat cgcttcattc atttaacgcc
      421 aactcctatt cgtcttgttc accgtcttca atgacgtccg ccagcttgtt tgtaaattgc
      481 tcagcggcgt tcagacccat ccgcttcaat ctgaagttca tcgctgccac ctcgatgatg
      541 actgcgagat ttcggcctgg acggacggga atcgtcagtt ttgtaatttc tgtatcaatg
      601 attttcatgg tttcttcctc aagtccgaga cggtcgtatt gcttgccctg ctcccagagt
      661 tcaagattca tgacaatcgt aattcgttta ttgcttctga cagcgccggc accaaacagc
      721 gtcatcacgt tgataatgcc aagtcccctg atttcaagga gatgctcaat taattcaggc
      781 gcgtttccga caagggtatc ctgatcctcc tgccgaattt caacgcaatc atcggcaacg
      841 aggcggtgcc cccttttcac aagctccagt gcggtttcac ttttcccgac gccgcttttt
      901 cccgtaatca gcacgccgac accgtaaata tcgacaagaa cgccatgaat cgctgtagta
      961 ggcgccagac ggctctcaag aaaattggtt aaacggcttg acagccttgt cgttttcagc
     1021 ggcgatctga ggacaggcac gccgtttttc tcggaagcgt caatcagctc ctgcgggata
     1081 ggcatatctc tggaaagaat aatagctggt gttacatcag tgcaaagaga atccattcgc
     1141 tgctttttat cctcttccgg cagctgttca aagaaagaaa gctctgtttt tccgagaagc
     1201 tgcacgcgct ctctcggata atatgtaaaa tatccggcaa tttcaatgcc tggtcttgat
     1261 aagtcactca ttgtaatcgg acggttaacc ccttcttctc cgctgattaa ttccaaattg
     1321 aactgttcca ttacgtcttt tgtgcgaacc tttgccacga tatgttcctc ctgttccggg
     1381 ctgccccgag cttgctcaca atactttcat tttatcactt tcgggcttga acctaaaaca
     1441 gattttataa aagacgggaa aacacctcag ctggtctaga tcactagtct gaaaaggggt
     1501 aaaataaatg tattcatatt ccagaaaggt ggatcaccta tggcagaaag tcttcttatc
     1561 aaaggcatcg cgatcgtgac agaaaatgaa gtaatcaaaa atggctatgt cggaatcaat
     1621 ggcggaaaaa tcagcacagt ttcaacagaa cggcctaaag agtcttactc aaaagagatt
     1681 caagcaccgg cagattccgt tttgcttccc gggatgattg acattcatat tcacggcggc
     1741 tatggcgcag atacgatgga tgcaagcttt tccgccctcg acaccatgtc ctcaagactt
     1801 ccagaagaag gaacgacgag ctttttagca acaaccatta cccaggaaca tgggcacatt
     1861 tcccaagctt tggtgagtgc aagagattgg aaagcagctg aagaatcctc tttattaggc
     1921 gcggaactgc tcggcattca tttggagggg ccctttgttt cacctaaaag agccggagcg
     1981 cagccaaagg aatggatcag gccttcggat gttgcgcttt tcaaaaagtg gcaacaggag
     2041 gcgggcggac tgatcaaaat cgtcacactt gcgccggagg aggatcagca ttttgaactg
     2101 atccgctatt tgaaagacga atcgattatc gcgtcgatgg ggcacacaga tgccgactcc
     2161 gcattactgt cggaggccgc gaaagcaggc gcgagccata tgacccatct ttacaacgcg
     2221 atgagcccgt ttcatcaccg agagcccgga gtgatcggaa cggcgctagc ccatgatggg
     2281 tttgtgacag agcttatcgc tgacggcatc cactcccatc ctttagcggc aaagcttgct
     2341 tttttggcaa aaggcagcag caagctcatt ttaattaccg attccatgag ggcaaaaggc
     2401 ctgaaagatg gtgtatacga attcggtggc caaagtgtga cggtaagagg aaagacagct
     2461 cttctttccg acggaacact tgccggttcg atccttaaaa tgaacgaagg cgcacggcat
     2521 atgcgtgagt ttacaaattg ctcttggacg gatatagcta atataacttc tgcaaacgcg
     2581 gccaaacagc tgggcatctt tgaccgaaaa ggcagtgtga cagagggaaa agatgcggat
     2641 cttgtgattg tcagcagtga ttgcgaggtg attctcacca tctgccgcgg aaacattgca
     2701 tttatatcca aggaggctga ccagatatga aagtagttga atgtcaaaca tatgaagagc
     2761 taagccaaaa agcagccaga atagcggcag acacaatcaa ggaaaaacct gacgccgttc
     2821 tcggtttagc aacaggcggc acaccggaag gcacgtatcg cgagctgatc cgcctgcacc
     2881 aaactgagaa tctttcattt aaaaacgtca ccacggttaa tttggatgaa tacgccggac
     2941 tttcaagcgg tgatccgaac agctatcact actatatgaa ttctcgtttt tttcagcata
     3001 tcgacagcca gccaagccgg cattttattc ccaatggaaa tgcagacgac ctggaagccg
     3061 aatgcaggcg gtacgaacaa ctagtcgaat ccattggcgg cactgacatt cagcttctcg
     3121 gcatcggccg aaatggacat atcggcttca acgaaccggg aacgcctttc aagtcgcgaa
     3181 cacacgttgt gacattaaat gaacaaaccc gccaggcgaa tgccagatat ttttcttcaa
     3241 tagacagtgt gccgaaaaaa gcactcacaa tgggaatcca gacgattctc tcaagcaagc
     3301 gcattctgct gcttatttcc ggcaaaagca aagcggaagc tgcgagaaag ctgttagaag
     3361 gagacgtaag cgaagatttc ccagcatcgg ctttgcacct gcaccccgat gttacgattt
     3421 tgattgatcg tgaagccgcg gcattaagac cttgaaagga acatgctgac ttatgaatat
     3481 cgataaacaa tcgcctattc cgatgtatta tcagattatg gagcaattaa aagcccaaat
     3541 taagagcgga gagctgcagc cagatatgcc tcttccctcc gagcgtgaat atgccgaaca
     3601 attcggaatc agccggatga cggttcgcca ggcgctttct aatttagtca atgaaggctt
     3661 tctttatcgc ctgaaaggac ggggcacctt tgtcagcaag ccaaaaatgg aacaagcact
     3721 ccaagggctt acaagcttta ccgaggatat gaaaagccgc gggatgacac cgggcagcag
     3781 gctcattgac taccagctta ttgagtcaac tgaggagctc gcagctttat taggctgcgg
     3841 gcatccctct cctatccata aaatcactcg agtgcggctg gcaaatgata ttccgatggc
     3901 aattgaatcc tcgcatattc cgttcgagct tgcgggtgaa ttaaacgaat cgcattttca
     3961 gtcgtcgatt tacgagcata ttgaaaagta caacagcatc ccgatttccc gtgcaaaaca
     4021 ggagcttgaa ccaagcgctg caacatcgaa agaagcaagc attcttggca ttcaaaaagg
     4081 ggcgcctgtc ctgttaatca aacgaacaac gtatttacag aacggaactg cttttgaaca
     4141 tgcaaaatca gtatacagag gcgaccgtta tacatttgtc cactatatgg accggctttc
     4201 ataaaaaaat ctccaaccct ttttaaggat tggagacatg gcgaaaatca aactggtctg
     4261 gcgacggacg atatgtctct tttttcgtct tgaacttcca gatcggtgat ttcgttttgc
     4321 cgttaaagct gtcttccaca atggtgtacc aataataaac agagtgcggt tcaagatcat
     4381 cccagcggaa ttcagcggta tccccgctct tcacttgctc ccgttttccg agctcttcat
     4441 tggtatatac attacattca aaataatcgg tttccacctt tttgacccta ggcttcaaat
     4501 caagcgaaag cgaaaactca tccttgttgc cgtaggtatc cgtatcatag tagtttttat
     4561 cttgtaaatg cggagagtac gtcgacacat gcaccatatc attcgcctgg tcaaactgca
     4621 gcaaacgcaa atacccttgt ccgccttctg gcccgccctg ataatcggcc agcatttgat
     4681 gcacgaggcg atccggtttt ccatcaccat catcatccag ctcatcagtt tttctcatcg
     4741 cactatgata atgcccgctt aacaccatca ccacattcgg attcggcttc acgacttttt
     4801 tgaatatctt ctctccgatc gggcttctgt ttccgctgac cagcaaatac tcatgaaaag
     4861 ctaaaacagc catccggtca ggatgcttct ttagcacttg attcatccac gcgatgtctt
     4921 catccgtaat gccccatccc atgtacagca tgatataatc attgccgttg ctcgaaatta
     4981 agtcataatg tccgcggtta tttttataag aaccgccata atgcaatttt ttatcaaaac
     5041 gatgactgcc aaagtatttg ccaaacgccc tgtaggagcc gtccttatgc ccgacatcgt
     5101 ggttacctgc cagcacgccg taaggaatcc cgcttttatc cagaacagac attgaccgat
     5161 cagcgttttt ccactgtctg atatcggcag aatcatctac aatatcaccc gtgtgaaaca
     5221 catatttgat attgagctgc ttttgattac ttttgatcca tgcggtctgt ttatcaaaaa
     5281 tatgcggata gctttccgcg taatactgcg tatcggacat ccaggcaaat gtgtacatat
     5341 ccttcgcagg aggaatttca tcctggacaa tcacattcat cttcgacttc cgcacaaaat
     5401 cggaggcgat tactgacgcc ttcagcgtaa acggtttgtg atctttggca acgaagctgt
     5461 caaccggctg ccactcgttt gtccggtaat tccaagcata catcgtcacc tttcttcccg
     5521 gtaaagaact acccttccag accgcttccg ccaagtcatt ctcatcaact gacggatcaa
     5581 ccgtcacttc aaagcgatga taagggaaaa gctccttctt ttctgtcgat actgtttttc
     5641 catcagcagc agatacccgc tccaattctt tattcgtaaa gggggtctcg ctttccggca
     5701 caaagctttt cggcggttca gtcagtgacg cgtgcttgaa tattttcaca ttgtttcgtg
     5761 ctgtatattg aaatcctcta taaaaagcga catccatggg gtcatccgtc ggatccttca
     5821 cccgcacaga aagcttagcc ctatgcgtat cggcttcgct gtctatgacc tccggctgat
     5881 ccggatgctc acgttctgtt ttaaacgtcc gctcaatgat tgttttattg cccgcgaggt
     5941 ccgttgcggt tatttttaga gaatgttttc ccggcaaaag ttcagaagat gacgtatggt
     6001 agggaagtgt aatgctctca ccatctaaag acgcctccgc ttcctcaatt ttgctccatt
     6061 tatcatacat atccgcctgc aggatgaggt ttcctttgta cgtttgtccc tcggtgatat
     6121 tcggctcgat atgcggcccc gaattatcta cacgcacaac caagcttaca ttttccttgc
     6181 cgtcgcttgc ttgaatatgg tgagcgcctt cagaaagctt tgatgtatcc cacttcagca
     6241 actgtgacgt aaacagtccg tcaggaagat tgaattgaaa ttgccagctt ttattcgaac
     6301 gctgattatc tccaataaag agttccttct caggagaaat cctctgatcc ctgatcaccg
     6361 tcccatccga taacaccaat cgggcgtttt tgactaagaa atcatcacgg ttttcagccg
     6421 tatcaaatgg agacaccttt gaacctgatc gtatcgtaat acgctgcagc tgcttccctt
     6481 tacggagcag caactctggt aagggaacag aataggtgcg gtatttattt gttgtatcat
     6541 caaatacctt taacactttg ccttccatcg ttaccgcgtt tttaaagtaa agattcgttt
     6601 ttctcgtatc gaacgcaaaa tacacttctt tttccatagc cggctctgtc acttgttttt
     6661 ttccgtctat ccacagctta atgctattag tgcgcccgtc tgttgtcgct ttgagaaact
     6721 gagtccccga tagcgtgtct ctgttttcaa tgttcatccg caaaccgtgc gatttgcttg
     6781 tttgtttaat atcaatcatt ttgaccggcg ttctcgcttc attgattccg tcccctgctg
     6841 ccacgtaata ctcaagctga tccttcccga tcagttcagg cgaatacaca atataatggt
     6901 agagattgtc gttgcggtct ttttcagcga gaatgcgctt aaacggtttg ttttcatctg
     6961 tccgatagta gaaggccgct gttttcacaa gactgcggtc ccgtatatca gcccgcagct
     7021 caatgctttc agcagggcga acaggcttgc ggtcagtcaa atcctcaatg accggctttt
     7081 ctttatcaag cttaattttt cgtttctgct caggaacttg cgcctgtaat accgttcccg
     7141 gggacggatt tttttctccg attgagatat tgagaagttc tgcggttccg tccagcggat
     7201 acttgtaaag cacagatgac agccggcgca gcgcatgttt ccgataatgc gcgaccgaaa
     7261 tatcttttcc tgtattcgtt gatatcacaa ctgaccgcgg cctagtattg gccatcccct
     7321 ctgtctcttc taatactgcg agatttttcc cttccgttaa ttgtgtctgg taatatcgat
     7381 tgaaatcctc cgccgtcaat tctggatgcc cgacaggctt cagccaaacc acaaaagttt
     7441 cgccggatgg aatggtgatc ctttcatccg gtctccaaat cagatcggag tcaggccctt
     7501 ctttcggata gcggtaacgg atatgatagt ctttaaaatc aatcgttctg tcagtcgtat
     7561 tataaatctc aataaattca taaccgtctg cacgtcccgt gttcttcgta tcaaccgcta
     7621 gctctgtgat cagaagctcc ggaatatggg cgagatcagc ctgatatcct tcaagcttca
     7681 gtttcttcgt ctccgactcg acccgctgct tcccattgga aatcacaatg taatactcaa
     7741 gctcgctgtt ccaaatcaat cgtttcggaa tttgcgccaa aaacttgtga atcgctccgg
     7801 gtgtcgggtc catcggcacc attttgtagc ccagctcatt actttgttta taaaaaagaa
     7861 aggcagaaaa tgcttgatac tcattgtcac gcacttgagc ctcaatcatc agattgcttt
     7921 tcatttcagc tttcacatcg atttgaggag gattttcaag caagggtatg taatgaggcg
     7981 tttggaatgg ttcctgcttt ataacaggga aagctttcga ttcatgatgg ccgccagaca
     8041 aaactgaaag aaaagacaag aacaaagaag cgataactgt gtatttcctc atcaaaacac
     8101 ctcccttttt acgtattttg cgtctgaata gggaagctta tgagttaatt tttgtgccaa
     8161 aagaacaaaa aagcgtctgc ctcttgaaaa tcccactatt acccagtaaa cttaggatag
     8221 ttaaccagtt aacaattaaa tcagagaggc agattgatgg acaacagtat tcatgatgaa
     8281 ttatttcaag ctatacaaca attcgcatta aaaagggata aacgcgtctg gcaaaaggta
     8341 caaataccat cccgcggcat gtcctcagca catctcgaac acctcaaaaa agactggaca
     8401 ttgacccagc ttcacattgt ttcctgtata cataccagcc aaaatgtgaa caacagcttc
     8461 ttagcatccc gcttacacat ctcaaaagca gctgtttcaa aagcagttca agctttgttg
     8521 aaacacaata tcatcacagt gaccaaaaaa cccggcaaca aaaaagaaat tttttatacg
     8581 ttaactgaaa gcggcagaga actggcggca ttacatgaac agcttcatga aaaagcaaag
     8641 gagcaataca aacagctctt caatgaattt tcaattgatg atttaaaaac agtgactgcg
     8701 tttttcaact tgtgggtcaa acatatgtaa aaaaagcctc acccgatggg agagaccttt
     8761 caatcttatt gagccctgtc aaacgcaata tgaagtgaga cgggtccgcg tgtatacaga
     8821 ccggactcag cataacgaaa accttcctct aacctgatat tccgcatctt atccagcaca
     8881 atattggcta caatctcgat ttcggtttta gcaaaagctg ctcctacaca gttatggatg
     8941 ccagatccga aagcgagatg ccgggcggcg ccgctaaaag cactcttgat gccgagatct
     9001 tcccgatgaa tattaaacac gtcaggatgt tcaaacgctt ccgggtcccg gttagctgct
     9061 ccgatcatac aaaaaacaat cgtatccttt ttgatttcca taccgccgac caccgtatct
     9121 tgggacagct gccgcggaat cagctgaacc ggcggtttat aacgcaacgt ctccgcaatg
     9181 gctctcggca ccaatgaacg gtcagccaaa acatcattca tctgctcggg attgttgagc
     9241 aaatgataaa tcatcagcgc cagcgtctta tctgccggtt ccgttgcggc taacagcaca
     9301 ttgagaatca gtgccaatat atccttgtcc gacatcgcca tgccttcata ttcagacgta
     9361 cacaggatgg aaattaaatc tgatcccgga ttgacgcgtc gttcctcgat gaccggcatc
     9421 aagtaatcgg aaagctgttc gctgcaccat aaagaatgcg cccgcgcttc aggagtctga
     9481 aagatactcg taataaaatc ggcaactccg ctgtgccact cagcgatttt ttcatgatct
     9541 cttttatcca gtccgagcat gtccatcgtg acacacaccg caaacgtctt tccaaaatca
     9601 ttgacgagat cgattctccc tttttctaga taaggatcta ataagttttc tgcattttgc
     9661 ttaatgaatg gagacagatg atccaatgcg tccccgacaa agcttctcac tacaattctt
     9721 ctttttgcgg agtgttcttt cccatgcatt tgggccagca cagggccgcg catgaccggt
     9781 tcagcacgct caacaagtga ttttgtcgta aaaatatccg gatgctgaag gatatagcgg
     9841 acatcatgat agcggctgat aaaataactg tctatcgact cttcataatg aacaggatct
     9901 tcctcccgca gctgtgaaaa ataagcatat ggattttctt gaaattgatc agaaagcaca
     9961 ctaaacaact taattgattg attcatgttt actcccccta tcatccttca gatgtgatcc
    10021 gtgtttcaga aagcatctgc gccgtttcct gcacaatgag atacccttga ttaggccgca
    10081 tacgaataga aaattcatgg ttactgatct tctcaccaag cttccacgga cgatgataag
    10141 caaggagcgt ctcttccacc tcaagaatat ccggagctcc gataaaaaac ggcagctcag
    10201 caatgacata ttccacagct aaattcagca tatcctcact gacttcttcc atcatcaaac
    10261 tgatgccgcg tgcacgcccg atcatcgctt cacgagacat gtccaaacaa gcatgccgaa
    10321 aatgaggctg ctcaaaaaac gcatgttcaa cttcttgtct catcagctga taggctttat
    10381 tttctgtaaa atcagaaaat gtatgaatcg ctttcagatc cccgccatga gccgcaagag
    10441 ccctttctgc aaatcttctg tttcgtgata cctcttttct tacttttcgt tcagctttgc
    10501 ctctgggagt tccgagcgct tcaagaagat tagccgcctc atgccccgca agcaaaacgg
    10561 aaacgctttt aaattcagcc gttgcccatc caatcaatct gtaaatataa tcctcagaaa
    10621 acctgctgtt aaatgggctg atccccacca aaacatgccg gcgccgttca aatatttctc
    10681 tgcagttttg tgtcagtgac tcagcagtaa aatctacagg ctttctttcc gtcaccattt
    10741 cggtcatctc attcacctct aaaaacttat cccgctttaa gtcatcaccc tcaaaaatga
    10801 gatggaataa ctggcacaac tgaacaagac gaatggcagg cagcacttaa agtttctaag
    10861 tgaattattt taagatttaa aacttctttt tcattattca tatcatgatc aatgtttact
    10921 agtaaacatt agtcatttta tcccttatcg ttccacccgt caatatgaat agtgcctatt
    10981 tttttgaaat aaatgtaaat gtcacatttt tcacacactt tttacactcc tgttcagcat
    11041 gacataacac cggataaagt ttaaacatca gcaattgaca acgaaagtaa gaaaagataa
    11101 aattaaatcg ttcaccagta aacaatttta tttattgttt catcgtaaac tattattcct
    11161 cagaaagaag gtagcctatg tctgatttga caaagcagat gatatacgac atatatgtga
    11221 gactgctgca ccttaatgaa caaaaagcaa acacttcact tcagcaattt tttaaggagg
    11281 ccgcagaaga ggatgtgatc gatatcccta aaaacatgac aagcattcat gtcattgact
    11341 gcatcggcca gcatgagccc attaacaatg cggggatcgc caaaaaaatg aacttatcga
    11401 aagcgaatgt aacgaaaatc agcacaaagc tgatcaagga agaattcatt aacagctatc
    11461 agctgacaga taacaaaaaa gaagtgtatt tcaaattaac ccgtaaaggc aaacggattt
    11521 tcgatttgca tgagaaactg cataaaaaaa aggagctggc cttttaccaa ttcctcgatt
    11581 cgttttcaca agatgagcaa aatgctgtat tgaaattttt agagcagctg acgtctacac
    11641 ttgaagcaga acaaaccgat gggactccgg acaaacctgt aaagtaaaca aaaggcggtg
    11701 tattctttat ggctcaaatg aaatcaaaag cggtattgat cttatacatt gtttgcttca
    11761 gcgcattttt tgcatcttta agccaaaaca tttattcacc tattatcccg atcattaaag
    11821 aatcgttcca tgtttctgca gcaatggtga acctgtcagt ctccgtcttt atgattgtga
    11881 cagcgattat gcaaattata ttagggacca tcatcgattt taaaggcgcg cggtttgtct
    11941 tgattgccgg aattctggca acgacagcag ccagcatcgg ctgtgcggtg accactgact
    12001 ttactttgtt tctgatattc agaatgatac aggctgccgg gtccgcagca ctgcctctca
    12061 ttgctgccac aacgatcgga cagcttttta caggcagtga acgcgggagt gcgatgggaa
    12121 cgtatcaaat gctcctttcc gccgcaccgg ctgtcgcccc tgtcctcggc ggattcatag
    12181 gcggagcagc cggatacgaa gggatcttct ggatgctcac ggccatctct gtcgttatgc
    12241 tggttgcaaa cagcatgatt tttcctaagg actctcccgc aggatctaag cagaaaacca
    12301 aaggcaatgt gttcactcat tacaaatcaa tatttacaaa tcgaacagga aacgtcattt
    12361 tggccttaag ttttgtcctc tttttcattt attttgcggt gatcgtctac ctcccgatat
    12421 tactgacgga gcattatcat attgatgtga gcatagcagg attgttatat ttgccactgg
    12481 cgctaagtac gatcgcaggt acgtttttgt ttaaaagaat acaggcaaaa atctcgctga
    12541 acacattgtt tatcggcagc aatgcggtcg cggcctgcag tatcatgttg ttcgccgtta
    12601 cacattccgt ttctctcgtt ctcatggctc ttacactggc actgtttggc atctcgatgg
    12661 gggtcattcc tcccttgtac tccacaatga tcaccaatga atttgaacac aacagaggga
    12721 gtgcaatcgg aattttcaac tttatcagat acacaggcat ggcggcaggt ccgatgatat
    12781 ctgcctatct gctcaccatg atgccgtctg ccatgtcctt tggactctta ggccttttat
    12841 ttgccgcatt gggcttttgc cttatgccgc aaatgtatcc gccgcagaag cgcgttaaaa
    12901 aaaaacacca catgtaaaaa agctgccttc tgtgggactg acccccgtt
//
