LOCUS       NZ_JALAVA010000025      1409 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain EC30O5B-F2-1 contig_0000025, whole
            genome shotgun sequence.
ACCESSION   NZ_JALAVA010000025
VERSION     NZ_JALAVA010000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 429.34545439822875x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:33:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,448
            Genes (coding)                    :: 4,297
            CDSs (with protein)               :: 4,297
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 84 of 151
            Pseudo Genes (incomplete)         :: 86 of 151
            Pseudo Genes (internal stop)      :: 28 of 151
            Pseudo Genes (multiple problems)  :: 40 of 151
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:09:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1409
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAVA010000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..>843
                     /locus_tag="MOE81_RS22525"
                     /old_locus_tag="MOE81_22475"
     source          1..1409
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="EC30O5B-F2-1"
                     /submitter_seqid="contig_0000025"
     region          1..1409
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1409
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1409
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2:>843](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..>843
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     CDS             3..>843
                     /NRPS_PKS="Domain: PCP (222-281). E-value: 6.3e-21. Score:
                     66.4. Matches aSDomain: nrpspksdomains_MOE81_RS22525_PCP.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389713.2"
                     /locus_tag="MOE81_RS22525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE81_22475"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_268397750.1"
                     /sec_met_domain="AMP-binding (E-value: 7.2e-51, bitscore:
                     160.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 9.6e-17, bitscore:
                     48.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHYGPSETHVVSTYTIHPGDPIPEFPPIGKPIGCTDLYILNHQKQ
                     LQPCGVPGELYISGASVARGYVNQDKLTRDKFSSDPFRPSAIMYRTGDLARRLGDGNIE
                     YIGRADNQVKIRGYRIEPQEIEVTLMNHPDIKEAAILISQDQNGEHELCAYYCSIQKLN
                     AIDLRSYMASELPEYMIPAKWIWVDSIPLTPNGKVDQAALPAPDASIIGNPYAAPRNLI
                     EAKLSQIFEDVLKNGHIGIQDNFFDHGGHSLKATVLMSRIAKEFHVQVPLQDIFAHPTV
                     "
     CDS_motif       145..234
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22525_0001"
                     /evalue="1.80E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="MOE81_RS22525"
                     /protein_end="77"
                     /protein_start="47"
                     /score="45.8"
                     /tool="antismash"
                     /translation="PCGVPGELYISGASVARGYVNQDKLTRDKF"
     CDS_motif       316..381
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22525_0002"
                     /evalue="6.50E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="MOE81_RS22525"
                     /protein_end="126"
                     /protein_start="104"
                     /score="40.5"
                     /tool="antismash"
                     /translation="YIGRADNQVKIRGYRIEPQEIE"
     aSDomain        670..>843
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22525_PCP.1"
                     /evalue="6.30E-21"
                     /label="MOE81_RS22525_PCP.1"
                     /locus_tag="MOE81_RS22525"
                     /protein_end="281"
                     /protein_start="222"
                     /score="66.4"
                     /tool="antismash"
                     /translation="EAKLSQIFEDVLKNGHIGIQDNFFDHGGHSLKATVLMSRIAKEFH
                     VQVPLQDIFAHPTV"
     assembly_gap    844..910
                     /estimated_length=67
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <911..>1409
                     /locus_tag="MOE81_RS22530"
                     /old_locus_tag="MOE81_22480"
     CDS             915..>1409
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (1-165). E-value:
                     3.1e-53. Score: 172.7. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22530_Condensation_LCL.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389713.2"
                     /locus_tag="MOE81_RS22530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE81_22480"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_268397751.1"
                     /sec_met_domain="Condensation (E-value: 9.4e-48, bitscore:
                     149.8, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRIYVLQQLDEGVAYNMPAVLELEGALDVAKLSAVCKELISRHE
                     PLRTSFVSEADGEPVQRIRTEVPFTLSTETAIEGFIRSFDLSQAPLFRAGLIKVTNEKH
                     VLLVDMHHIISDGVSVQLLIREFTDLYANRQLKPLRIQYKDYAVWQQQFKKGDSYQKQE
                     TY"
     aSDomain        920..>1409
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22530_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="3.10E-53"
                     /label="MOE81_RS22530_Condensation_LCL.1"
                     /locus_tag="MOE81_RS22530"
                     /protein_end="165"
                     /protein_start="1"
                     /score="172.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="KRIYVLQQLDEGVAYNMPAVLELEGALDVAKLSAVCKELISRHEP
                     LRTSFVSEADGEPVQRIRTEVPFTLSTETAIEGFIRSFDLSQAPLFRAGLIKVTNEKHV
                     LLVDMHHIISDGVSVQLLIREFTDLYANRQLKPLRIQYKDYAVWQQQFKKGDSYQKQET
                     Y"
     CDS_motif       959..1072
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22530_0001"
                     /evalue="2.80E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="MOE81_RS22530"
                     /protein_end="52"
                     /protein_start="14"
                     /score="51.9"
                     /tool="antismash"
                     /translation="AYNMPAVLELEGALDVAKLSAVCKELISRHEPLRTSFV"
     CDS_motif       1244..1309
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22530_0002"
                     /evalue="9.00E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="MOE81_RS22530"
                     /protein_end="131"
                     /protein_start="109"
                     /score="34.2"
                     /tool="antismash"
                     /translation="MHHIISDGVSVQLLIREFTDLY"
     CDS_motif       1340..1372
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22530_0003"
                     /evalue="5.50E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="MOE81_RS22530"
                     /protein_end="152"
                     /protein_start="141"
                     /score="15.7"
                     /tool="antismash"
                     /translation="QYKDYAVWQQQ"
ORIGIN
        1 caatcattac ggaccttcag aaactcatgt ggtatctacg tacaccattc atccgggaga
       61 ccctattcct gagtttccgc caatcggtaa accgataggc tgtactgatt tatatatttt
      121 gaatcaccaa aaacagctac agccatgcgg tgtaccaggt gaactctata tttccggcgc
      181 aagtgttgcc agaggatatg tcaatcaaga taaactgaca cgcgacaaat tttcatccga
      241 cccattcagg cctagtgcga tcatgtatcg tacaggtgat ttggccagac ggcttggaga
      301 cggcaacatc gaatatatag gacgggcaga caatcaagtg aaaatcagag gttatcgaat
      361 cgagccgcaa gaaatcgaag tcacgttaat gaatcatccg gacattaaag aggctgccat
      421 tctcatcagt caagatcaaa acggagaaca tgaattatgc gcttactatt gcagtataca
      481 aaagctgaat gcaatagatt tacgcagcta tatggctagt gagctgccag aatacatgat
      541 tcccgcgaaa tggatatggg ttgacagcat acctcttacc ccaaacggaa aagtagatca
      601 agcagcgctt ccagcgccag atgcttcaat catcggaaat ccatatgctg cccctcgtaa
      661 tctgatagag gcaaagcttt cacaaatatt tgaagatgtg ctgaagaatg gtcatatagg
      721 catccaagac aacttttttg accatggagg ccactctcta aaagcaacag tactgatgtc
      781 acggatcgca aaggaatttc atgtacaagt tccgcttcag gacatctttg cccatccgac
      841 tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn cacaaaaacg gatatatgtg cttcagcagc ttgatgaggg tgtcgcctac
      961 aatatgcctg cggtcttaga actggaaggg gcactggatg tcgctaaact gtctgctgta
     1021 tgtaaagaac tgatcagccg gcatgagcct cttcgaactt cctttgtatc cgaggcagat
     1081 ggcgagccgg ttcagcgtat ccgcaccgaa gtgccgttta cattaagtac agagaccgct
     1141 atcgaggggt ttatccgctc ctttgatctc agccaggcgc cattatttag agcgggcctt
     1201 ataaaagtga cgaacgagaa gcatgtattg ctggtggata tgcaccatat catttcagat
     1261 ggcgtatccg ttcagctcct aatccgagaa ttcaccgatc tttatgcaaa ccggcagctt
     1321 aagccattgc ggatccagta taaagattac gccgtctggc agcagcaatt taaaaaggga
     1381 gactcgtacc aaaagcagga gacttactg
//
