LOCUS       NZ_JALAVA010000015      8416 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain EC30O5B-F2-1 contig_0000015, whole
            genome shotgun sequence.
ACCESSION   NZ_JALAVA010000015
VERSION     NZ_JALAVA010000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 429.34545439822875x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:33:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,448
            Genes (coding)                    :: 4,297
            CDSs (with protein)               :: 4,297
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 84 of 151
            Pseudo Genes (incomplete)         :: 86 of 151
            Pseudo Genes (internal stop)      :: 28 of 151
            Pseudo Genes (multiple problems)  :: 40 of 151
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:09:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8416
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAVA010000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..8416
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<1545:8326](-)"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1546..8326)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
     gene            complement(<1..548)
                     /locus_tag="MOE81_RS22445"
                     /old_locus_tag="MOE81_22395"
     CDS             complement(<1..548)
                     /NRPS_PKS="Domain: PP-binding (44-110). E-value: 7.2e-15.
                     Score: 47.0. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22445_PP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (128-181). E-value: 4.4e-11.
                     Score: 34.9. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22445_PP-binding.2"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /locus_tag="MOE81_RS22445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE81_22395"
                     /product="acyl carrier protein"
                     /protein_id="WP_268291526.1"
                     /sec_met_domain="PP-binding (E-value: 2.1e-15, bitscore:
                     45.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGQWKHIYRQLKEKEITPEEASWQFKQWGKHKEKNHAANDLLPA
                     LKEIAAEVLAIDANEIHHDVLLGEQGFDPLKLNQLANRLNTKFGLELTTKDLAEQNTLD
                     DLAQFLQAAKSSQGDQRLREKTINYLKEHLSSVFKINAEEIASDIDLEKYGINSIMVLK
                     LTQQLEGFFGPLTKTLFFE"
     source          1..8416
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="EC30O5B-F2-1"
                     /submitter_seqid="contig_0000015"
     region          1..8416
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="transAT-PKS-like"
                     /region_number="1"
                     /rules="cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS
                     or itr_KS or tra_KS) and not PKS_AT)"
                     /tool="antismash"
     cand_cluster    1..8416
                     /SMILES="CC(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(ATd and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /kind="single"
                     /product="transAT-PKS-like"
                     /protoclusters="1"
                     /tool="antismash"
     aSDomain        complement(6..164)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22445_PP-binding.2"
                     /evalue="4.40E-11"
                     /label="MOE81_RS22445_PP-binding.2"
                     /locus_tag="MOE81_RS22445"
                     /protein_end="181"
                     /protein_start="128"
                     /score="34.9"
                     /tool="antismash"
                     /translation="YLKEHLSSVFKINAEEIASDIDLEKYGINSIMVLKLTQQLEGFFG
                     PLTKTLFF"
     aSDomain        complement(219..416)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22445_PP-binding.1"
                     /evalue="7.20E-15"
                     /label="MOE81_RS22445_PP-binding.1"
                     /locus_tag="MOE81_RS22445"
                     /protein_end="110"
                     /protein_start="44"
                     /score="47.0"
                     /tool="antismash"
                     /translation="ALKEIAAEVLAIDANEIHHDVLLGEQGFDPLKLNQLANRLNTKFG
                     LELTTKDLAEQNTLDDLAQFL"
     gene            complement(562..1515)
                     /locus_tag="MOE81_RS22450"
                     /old_locus_tag="MOE81_22400"
     CDS             complement(562..1515)
                     /NRPS_PKS="Domain: PKS_DH2 (21-251). E-value: 3.9e-58.
                     Score: 188.5. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22450_PKS_DH2.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /locus_tag="MOE81_RS22450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE81_22400"
                     /product="polyketide synthase dehydratase domain-containing
                     protein"
                     /protein_id="WP_248596971.1"
                     /transl_table=11
                     /translation="MHPLVHLNTSDLSTQRFTTILTGKEAYLTDHQVKERRVLPGSAYL
                     EMARTALEQGVDRVTTDTLQQGTVFICLQEHIWIAPLVVEDKPVEVHITLALIENGNVE
                     YEIYSGSDHPDDQSAILHAQGVLKVSELTPPKRQSLQQLKSQYPWRTLLPENIYPLFRE
                     AGLYYGPSHSGIERLYLHKDQILAKISVLPESRGDRECYLDPGLLDAVFQATAGFAISL
                     NAPLDSLPFTLKEMYIYQPCPKQVWAYIRQREKGRVFDIDVYDENGMVCVRILGFETRR
                     GAGLMSEEAFYRHLSERIWNGELSQEEVERILMASR"
     aSDomain        complement(763..1452)
                     /aSDomain="PKS_DH2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22450_PKS_DH2.1"
                     /evalue="3.90E-58"
                     /label="MOE81_RS22450_PKS_DH2.1"
                     /locus_tag="MOE81_RS22450"
                     /protein_end="251"
                     /protein_start="21"
                     /score="188.5"
                     /tool="antismash"
                     /translation="TGKEAYLTDHQVKERRVLPGSAYLEMARTALEQGVDRVTTDTLQQ
                     GTVFICLQEHIWIAPLVVEDKPVEVHITLALIENGNVEYEIYSGSDHPDDQSAILHAQG
                     VLKVSELTPPKRQSLQQLKSQYPWRTLLPENIYPLFREAGLYYGPSHSGIERLYLHKDQ
                     ILAKISVLPESRGDRECYLDPGLLDAVFQATAGFAISLNAPLDSLPFTLKEMYIYQPCP
                     KQVWAYIR"
     CDS_motif       complement(1360..1437)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22450_0001"
                     /evalue="2.50E-11"
                     /label="PKSI-DH_m2"
                     /locus_tag="MOE81_RS22450"
                     /protein_end="52"
                     /protein_start="26"
                     /score="35.9"
                     /tool="antismash"
                     /translation="YLTDHQVKERRVLPGSAYLEMARTAL"
     gene            complement(<1546..>3279)
                     /locus_tag="MOE81_RS22455"
                     /old_locus_tag="MOE81_22405"
                     /pseudo=""
     CDS             complement(<1546..3279)
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (2-422). E-value:
                     5.9e-177. Score: 580.7. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22455_PKS_KS.1"
                     /NRPS_PKS="Domain: Trans-AT_docking (438-575). E-value:
                     2.8e-29. Score: 94.4. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22455_Trans-AT_docking.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: ATd"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: tra_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352336.1"
                     /locus_tag="MOE81_RS22455"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="MOE81_22405"
                     /product="type I polyketide synthase"
                     /pseudo=""
                     /sec_met_domain="tra_KS (E-value: 1.1e-183, bitscore:
                     598.9, seeds: 44, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.2e-94, bitscore:
                     303.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="ATd (E-value: 1.9e-30, bitscore: 94.4,
                     seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIAIIGMSGRYPEADNLDAYWSNLKAGRDSIREIPPSRWDVASF
                     YQPFPSDEGKIYSKWLGLMDDVACFDPLFFNIAPSEAETMDPQQRLFLQEGYQAFADAG
                     YTPEALNGQNCGVYLGIMANEYYRMLCEQQYGLTDMTGNYASIASARIAYFLNLKGPAL
                     SIDTACSSSLVAINLACEALLNRKIDMALAGGVTLYLTPKPYIEMCAAHMLSPEGRCKA
                     FDKSSDGMVPGEGVGAVVLKRLQDAEADNDLIYGVIIGFGINQDGKTNGLTAPSVKSQI
                     ALERSIYQNYNIDPSSISYAEMHGTGTQLGDPIELKALSTAFQEKTDKKQFCAIGSVKS
                     NIGHTSAAAGVASVHKVLLSMQHQQLVPTLHFKEPNEYFDFKNSPFYVNTQTRPWDANS
                     PRRACVSSFGFSGTNAHLVIEAYETKKKRVRMPVTDASRPALFVLSAKTEKQLKVYAGI
                     MKEWIASQVDVNLKDMTYTLQTSREAMDYRMAFWMDSKFAAIDMLTAYLNDEQPDGLHI
                     SHVKKRKGQRNTSLHTDPETLFSQWLAGEWVDWQVFYKDDEPRHIRLPGYPFAKEHYWL
                     PK"
     aSDomain        complement(1555..1965)
                     /aSDomain="Trans-AT_docking"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22455_Trans-AT_docking.1
                     "
                     /evalue="2.80E-29"
                     /label="MOE81_RS22455_Trans-AT_docking.1"
                     /locus_tag="MOE81_RS22455"
                     /protein_end="575"
                     /protein_start="438"
                     /score="94.4"
                     /tool="antismash"
                     /translation="PALFVLSAKTEKQLKVYAGIMKEWIASQVDVNLKDMTYTLQTSRE
                     AMDYRMAFWMDSKFAAIDMLTAYLNDEQPDGLHISHVKKRKGQRNTSLHTDPETLFSQW
                     LAGEWVDWQVFYKDDEPRHIRLPGYPFAKEHYW"
     aSModule        complement(1555..3273)
                     /domains="nrpspksdomains_MOE81_RS22455_PKS_KS.1"
                     /domains="nrpspksdomains_MOE81_RS22455_Trans-AT_docking.1"
                     /incomplete
                     /locus_tags="MOE81_RS22455"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(2014..3273)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22455_PKS_KS.1"
                     /domain_subtypes="Trans-AT-KS"
                     /domain_subtypes="exomethylene"
                     /evalue="5.90E-177"
                     /label="MOE81_RS22455_PKS_KS.1"
                     /locus_tag="MOE81_RS22455"
                     /protein_end="422"
                     /protein_start="2"
                     /score="580.7"
                     /specificity="transATor: exomethylene"
                     /tool="antismash"
                     /translation="IAIIGMSGRYPEADNLDAYWSNLKAGRDSIREIPPSRWDVASFYQ
                     PFPSDEGKIYSKWLGLMDDVACFDPLFFNIAPSEAETMDPQQRLFLQEGYQAFADAGYT
                     PEALNGQNCGVYLGIMANEYYRMLCEQQYGLTDMTGNYASIASARIAYFLNLKGPALSI
                     DTACSSSLVAINLACEALLNRKIDMALAGGVTLYLTPKPYIEMCAAHMLSPEGRCKAFD
                     KSSDGMVPGEGVGAVVLKRLQDAEADNDLIYGVIIGFGINQDGKTNGLTAPSVKSQIAL
                     ERSIYQNYNIDPSSISYAEMHGTGTQLGDPIELKALSTAFQEKTDKKQFCAIGSVKSNI
                     GHTSAAAGVASVHKVLLSMQHQQLVPTLHFKEPNEYFDFKNSPFYVNTQTRPWDANSPR
                     RACVSSFGFSGTNAHLVIEAY"
     CDS_motif       complement(2230..2274)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22455_0003"
                     /evalue="3.50E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="MOE81_RS22455"
                     /protein_end="350"
                     /protein_start="335"
                     /score="12.9"
                     /tool="antismash"
                     /translation="GSVKSNIGHTSAAAG"
     CDS_motif       complement(2335..2382)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22455_0002"
                     /evalue="1.40E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="MOE81_RS22455"
                     /protein_end="315"
                     /protein_start="299"
                     /score="13.8"
                     /tool="antismash"
                     /translation="YAEMHGTGTQLGDPIE"
     CDS_motif       complement(2755..2802)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22455_0001"
                     /evalue="6.20E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="MOE81_RS22455"
                     /protein_end="175"
                     /protein_start="159"
                     /score="21.2"
                     /tool="antismash"
                     /translation="GPALSIDTACSSSLVA"
     gene            complement(3395..8326)
                     /locus_tag="MOE81_RS22460"
                     /old_locus_tag="MOE81_22410"
     CDS             complement(3395..8326)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: ACP_beta (149-216). E-value: 5e-29.
                     Score: 91.9. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22460_ACP_beta.1"
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (751-1173). E-value:
                     3.4e-177. Score: 581.5. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22460_PKS_KS.1"
                     /NRPS_PKS="Domain: Trans-AT_docking (1189-1331). E-value:
                     5.1e-40. Score: 129.3. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22460_Trans-AT_docking.1"
                     /NRPS_PKS="Domain: ACP_beta (1430-1496). E-value: 2.3e-25.
                     Score: 80.2. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22460_ACP_beta.2"
                     /NRPS_PKS="Domain: ACP_beta (1526-1591). E-value: 7e-28.
                     Score: 88.2. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22460_ACP_beta.3"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: ATd"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: tra_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR02814.1"
                     /locus_tag="MOE81_RS22460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE81_22410"
                     /product="PfaD family polyunsaturated fatty acid/polyketide
                     biosynthesis protein"
                     /protein_id="WP_268274641.1"
                     /sec_met_domain="tra_KS (E-value: 3.2e-186, bitscore:
                     607.3, seeds: 44, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.2e-92, bitscore:
                     296.7, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="ATd (E-value: 3.4e-41, bitscore: 129.3,
                     seeds: 19, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.7e-15, bitscore:
                     45.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPNSYSGEVSEGFMSQKVMELFGSDLIQQSHNAATSEQYQEILQG
                     LSNLYCQGYDLPWKQMWKEEPERVHLPTYPFARNSYWAPKASEPEQTPVVDSSQPEIAT
                     ALSVVANDKTISIPASSPVEMVLPDSKQDSIEEESYEEQGLEELQEELADSLAEALYIE
                     REMILPGKKFIELGLDSIVGVEWIKTLNKTYGIAIKATKVYDYPTLLDFTQYVASELGR
                     LKKRPNKQKPLSAEGKGDTKETLKAPIMKIDASPTSIHGNKNPALLNQKPASPGVKKNV
                     IRPESLGDKLFQTRYRCKWNYMAGSMAQGVASEALVIAMAEANLLSFFGSAGLDGDRLA
                     SHIQHIQSVVGPNKPYGMCLIANVNNLEEEWEQAQIFITHGVPFIEASAFSSITAPLVY
                     CRVKGIKKQKDGILYFPRRIIGKCSRLEIAKQFMAPPPEDLVDQLLEEGLITAEEAVLS
                     RSIPMVDDIVLEADSGGHTDRGVLSALVPSVLALRKDIQQTYSYPEPILVGCGGGIGTP
                     EAVASAFMMGADFVLTGSINQCTVESGAHDVIKELLSTISIHDTAVTVAGDMFEIGAKA
                     QVVRKHTQFYNRANRLYELFMQYNAIEEIPQEIKQEIEINYFKRTFAEVWDLVCEYKKK
                     KNPQQLTEASVNPRFKMALIFKWYFAYCNRATLNGDLTEKDNFQIFCGPALGAFNQWVK
                     GTPYEDWKKRHVVDVAVLLMEQACEHLQTRYSPRAVAVETPSIQQRAIEDPRLKETDIA
                     IVGISGQFPKADNINAFWENIISGKDCISEIPPSRWPMAHYYDTDPEVPGKSTSKWMGV
                     LEDVDKFDPNFFNIPPVDAIAMDPQQRMFLENCWSCIEDAGIRPSDLSGSQCGVFVGCA
                     TSDYGQLFNEQNLNSRMLMGNTTSILAARISYLLNLSGPSLAIETACSSSLVAVAEACN
                     SLVLGNSDLALAGGVCMMAGPSLHIMTSKAGMLSQQGRCRTFDQQADGFVPGEGVGVVL
                     LKRLSDAIRDKDTIYGVIKGWGVNQDGKTNGITAPNGQAQTDLEKSVYEKFGIDPADIS
                     LVEAHGTGTKLGDPIEVEALTEAFQSFTTEKQYCALGSVKSNIGHLLTAAGIAGLIKVL
                     LAIKHRTLPPTIHYDQLNEHIDLADSPFFISKNKEPWTTRQGKPRSAAVSSFGFSGTNA
                     HMVVEEYLPLEESDTAINYHVDQPVLMVLSAKNDKQLKHYADRMIHFIQKNPAIDLQQM
                     AYTLQVGREPMECRMAFLVHSSEELLQTFRMYRLGQQPSNLVTGTLTRENKKPDKRLNK
                     TFEEGQWHEAALWWIQGGEVDWKLLYGDHLPHRINLPTYPFERKSFWPKKDDKTLIENV
                     KVSPSQMPEPVASLPSKTKQTVSLQEVEQQTLKPDQIQLRALKNMPDWPDHHQEQVLSP
                     VTLYKIDQPIDYHQVSHYAEEDEAALQDMLIDSLVQTLFIEGETVDLDQNFYDIGLDSI
                     LGVEWMRLINKTQGLSLEATTLYDYPTIRELARYILSMQGVDAQPQANSYLTTEQADAD
                     DTALIVELSKSLAEIMFLDPSRIDEDVHFSDLGLDSILGVEWIRVLNKRYRTTIEATNL
                     YDYPTLREFAAWMQKDQVKQSIPSTVEEEPALSPPTLEEILQKVEQGALEVTEAHVLLQ
                     KYNLV"
     aSDomain        complement(3554..3748)
                     /aSDomain="ACP_beta"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22460_ACP_beta.3"
                     /evalue="7.00E-28"
                     /label="MOE81_RS22460_ACP_beta.3"
                     /locus_tag="MOE81_RS22460"
                     /protein_end="1591"
                     /protein_start="1526"
                     /score="88.2"
                     /tool="antismash"
                     /translation="ELSKSLAEIMFLDPSRIDEDVHFSDLGLDSILGVEWIRVLNKRYR
                     TTIEATNLYDYPTLREFAAW"
     aSDomain        complement(3839..4036)
                     /aSDomain="ACP_beta"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22460_ACP_beta.2"
                     /evalue="2.30E-25"
                     /label="MOE81_RS22460_ACP_beta.2"
                     /locus_tag="MOE81_RS22460"
                     /protein_end="1496"
                     /protein_start="1430"
                     /score="80.2"
                     /tool="antismash"
                     /translation="MLIDSLVQTLFIEGETVDLDQNFYDIGLDSILGVEWMRLINKTQG
                     LSLEATTLYDYPTIRELARYI"
     aSModule        complement(3839..6073)
                     /complete
                     /domains="nrpspksdomains_MOE81_RS22460_PKS_KS.1"
                     /domains="nrpspksdomains_MOE81_RS22460_Trans-AT_docking.1"
                     /domains="nrpspksdomains_MOE81_RS22460_ACP_beta.2"
                     /locus_tags="MOE81_RS22460"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(4334..4759)
                     /aSDomain="Trans-AT_docking"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22460_Trans-AT_docking.1
                     "
                     /evalue="5.10E-40"
                     /label="MOE81_RS22460_Trans-AT_docking.1"
                     /locus_tag="MOE81_RS22460"
                     /protein_end="1331"
                     /protein_start="1189"
                     /score="129.3"
                     /tool="antismash"
                     /translation="PVLMVLSAKNDKQLKHYADRMIHFIQKNPAIDLQQMAYTLQVGRE
                     PMECRMAFLVHSSEELLQTFRMYRLGQQPSNLVTGTLTRENKKPDKRLNKTFEEGQWHE
                     AALWWIQGGEVDWKLLYGDHLPHRINLPTYPFERKSFW"
     aSDomain        complement(4808..6073)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22460_PKS_KS.1"
                     /domain_subtypes="Trans-AT-KS"
                     /domain_subtypes="beta-Me"
                     /evalue="3.40E-177"
                     /label="MOE81_RS22460_PKS_KS.1"
                     /locus_tag="MOE81_RS22460"
                     /protein_end="1173"
                     /protein_start="751"
                     /score="581.5"
                     /specificity="transATor: beta-Me"
                     /tool="antismash"
                     /translation="IAIVGISGQFPKADNINAFWENIISGKDCISEIPPSRWPMAHYYD
                     TDPEVPGKSTSKWMGVLEDVDKFDPNFFNIPPVDAIAMDPQQRMFLENCWSCIEDAGIR
                     PSDLSGSQCGVFVGCATSDYGQLFNEQNLNSRMLMGNTTSILAARISYLLNLSGPSLAI
                     ETACSSSLVAVAEACNSLVLGNSDLALAGGVCMMAGPSLHIMTSKAGMLSQQGRCRTFD
                     QQADGFVPGEGVGVVLLKRLSDAIRDKDTIYGVIKGWGVNQDGKTNGITAPNGQAQTDL
                     EKSVYEKFGIDPADISLVEAHGTGTKLGDPIEVEALTEAFQSFTTEKQYCALGSVKSNI
                     GHLLTAAGIAGLIKVLLAIKHRTLPPTIHYDQLNEHIDLADSPFFISKNKEPWTTRQGK
                     PRSAAVSSFGFSGTNAHMVVEEY"
     CDS_motif       complement(5135..5179)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22460_0003"
                     /evalue="2.00E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="MOE81_RS22460"
                     /protein_end="1064"
                     /protein_start="1049"
                     /score="13.4"
                     /tool="antismash"
                     /translation="VEAHGTGTKLGDPIE"
     CDS_motif       complement(5375..5413)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22460_0002"
                     /evalue="1.60E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="MOE81_RS22460"
                     /protein_end="984"
                     /protein_start="971"
                     /score="11.7"
                     /tool="antismash"
                     /translation="FDQQADGFVPGEG"
     CDS_motif       complement(5555..5602)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22460_0001"
                     /evalue="4.60E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="MOE81_RS22460"
                     /protein_end="924"
                     /protein_start="908"
                     /score="18.6"
                     /tool="antismash"
                     /translation="GPSLAIETACSSSLVA"
     aSDomain        complement(7679..7879)
                     /aSDomain="ACP_beta"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22460_ACP_beta.1"
                     /evalue="5.00E-29"
                     /label="MOE81_RS22460_ACP_beta.1"
                     /locus_tag="MOE81_RS22460"
                     /protein_end="216"
                     /protein_start="149"
                     /score="91.9"
                     /tool="antismash"
                     /translation="EELADSLAEALYIEREMILPGKKFIELGLDSIVGVEWIKTLNKTY
                     GIAIKATKVYDYPTLLDFTQYV"
ORIGIN
        1 tattcaaaga acagggtttt agtcaatggg ccaaagaacc cttccaactg ctgggtcaat
       61 tttaatacca taatggagtt aataccatac ttctctaagt ctatatcgct ggctatttcc
      121 tcggcattga tcttaaacac cgaggaaagg tgctctttta aatagttaat ggttttttcc
      181 cgcagacgct gatccccttg agatgattta gcagcctgta aaaactgagc tagatcatcc
      241 agtgtgttct gttccgctaa atccttagtg gttaactcta gtccaaactt agtatttagc
      301 cgattcgcca attgattcag ctttaaaggg tcaaatcctt gttctcccaa taggacatca
      361 tgatggatct cgttggcgtc aatagccaac acttctgcag ctatttcctt taacgccggc
      421 aataaatcgt tagccgcgtg attcttctcc ttatgtttgc cccattgctt aaattgccat
      481 gaagcttcct ctggtgtaat ctccttttcc tttaactgcc gatagatatg tttccactgt
      541 ccatccattt ttcctctccc cttatcttga tgccattaaa atccgctcga cttcttcttg
      601 cgataattca ccattccaaa ttctctccga tagatggcga taaaaagctt cttctgacat
      661 tagtcccgct ccccgccgcg tttcaaagcc taaaatacgt acacatacca tgccattctc
      721 atcgtagaca tcgatatcaa atacccgtcc tttctccctt tgccggatgt aggcccacac
      781 ctgtttaggg cacggctgat agatatacat ttctttaaga gtaaaaggca gactatctaa
      841 aggcgcattt aatgaaatag caaaacctgc cgtcgcttga aaaacagcat ccagtaggcc
      901 cggatcaagg tagcattccc tatctccgcg tgattccggc agtactgata tcttcgccaa
      961 aatctgatcc ttatggagat aaagacgttc aatcccacta tgggagggac cataatatag
     1021 gcccgcttca cgaaaaagcg gatagatatt ctccggtaag agggtgcgcc aaggatactg
     1081 agattttaac tgttgaaggc tttgtctttt agggggggtt aactcagata ccttcaaaac
     1141 accttgggca tgcaatatag ctgattgatc atcgggatga tccgatccgc tgtagatttc
     1201 atattctaca ttcccattct ctattagtgc caaggtaata tgcacttcaa ccggtttatc
     1261 ttccaccacc aacggtgcaa tccatatatg ctcttgtaag caaataaaga ccgtgccttg
     1321 ttgtaaagta tcggtagtta cccgatccac cccttgctca agggccgttc ttgccatttc
     1381 cagataggct gatccaggta acacccttct ctccttcacc tggtgatcag ttaaataggc
     1441 ttcctttcct gtcaggatgg tcgtaaaccg ctgagtcgac agatcagatg tattgagatg
     1501 aactaaaggg tgcaaggtac tgttacttga ggatgttgtg tcctgcttag gaagccaata
     1561 atgttccttg gcaaaaggat agcccggcag acggatatgt cgtggctcgt catccttata
     1621 aaaaacctgc caatctaccc attcaccggc cagccactgt gagaaaagtg tttcaggatc
     1681 agtatgtaga gatgtatttc tctgaccctt ccgctttttt acatggctga tgtgtaaccc
     1741 atccggttgt tcatcattca gataagccgt caacatatcg atggctgcaa atttgctatc
     1801 catccaaaag gccatgcggt aatccatggc ctcccgactc gtctgtaatg tgtaggtcat
     1861 atctttgaga ttcacatcca cttgtgatgc aatccattct ttcattatgc ccgcatatac
     1921 tttaagttgt ttctccgttt tagcagataa aacaaataaa gccggccggc tggcatctgt
     1981 tactggcatc cgcactcttt ttttctttgt ttcgtaggct tctataacca aatgagcatt
     2041 ggtgccgcta aaaccaaatg aactgacaca agctcgtctt ggtgaatttg catcccaagg
     2101 acgagtctgt gtattgacat aaaaagggga gtttttaaaa tcaaagtatt catttggctc
     2161 tttaaagtga agagtgggca ccaactgctg atgctgcata cttaacaaca ctttatgaac
     2221 gctggctaca ccagcggcgg ctgatgtgtg accgatattg gatttgacgg aaccaatggc
     2281 gcaaaactgc tttttatcgg ttttttcttg aaatgcagtt gataatgctt taagttcaat
     2341 aggatcacct aattgggtac ccgtgccatg catttctgca tagctaatgc ttgaaggatc
     2401 aatgttatag ttttggtaga tacttcgctc caaggctatc tggcttttca cactaggagc
     2461 agttagacca ttcgtttttc catcttgatt aataccaaat ccaataatca cgccataaat
     2521 taagtcatta tcagcctcag catcctggag acgctttaac acgacagcac caacgccttc
     2581 accgggaacc atgccatcac tggatttatc aaaagccttg catcgcccct ctggtgaaag
     2641 catgtgtgcc gcgcacattt caatatatgg cttgggggtg aggtacaacg ttacccctcc
     2701 cgcaagagcc atatcaattt tccggttaag taacgcttca cacgcaaggt tgatcgctac
     2761 caaagaggac gaacatgcag tatcaataga aagcgccgga ccttttagat taagaaagta
     2821 ggcaatacgc gccgatgcaa tagaagcata attgccagtc atatcagtta agccatattg
     2881 ttgttcacac aacatccgat aatattcatt cgccatgatg ccaagataaa ctccgcaatt
     2941 ctgaccattc aatgcctctg gcgtatagcc ggcatctgca aaagcctgat aaccttcctg
     3001 aagaaagagt cgttgttgcg gatccatggt ttctgcctct gaaggcgcaa tattgaaaaa
     3061 caggggatca aagcaggcca catcatccat caaacctagc cacttactgt atattttccc
     3121 ttcatcactg ggaaacggtt gatagaatga agcaacatcc caacgtgaag gcggtatttc
     3181 cctgatagaa tcgcggcctg ctttaagatt tgaccaatag gcatcgagat tgtccgcttc
     3241 tggatatctc cctgacatcc caatgatggc aatggcctcc cggtgttcgc cagacttagg
     3301 attcgtcaat tggctttttg cctcttcaga tgatatcttt cccgcttgca gggctcgtaa
     3361 gatggcttgt gaatttacca tttcccttcc ccccttaaac cagattgtat ttttgtaaca
     3421 ggacatgcgc ctccgttact tccaatgcgc cctgctctac tttttgcaaa atctcttcca
     3481 atgtgggcgg ggatagcgcc ggttcctctt ccacagtgga tggaatactc tgtttgactt
     3541 gatctttttg catccaagcg gcaaactcgc gtaaggtcgg atagtcatat aagttcgttg
     3601 cctcgattgt ggttcgatag cgtttattta acacccgaat ccattccaca cctaaaatag
     3661 agtctagacc caagtctgaa aaatggacat cttcatcaat ccgactggga tcaaggaaca
     3721 taatttcagc cagacttttc gacaactcga ctatcaaagc cgtgtcatct gcatccgctt
     3781 gttctgttgt taaataggag ttcgcttgtg gctgtgcatc caccccctgc atagatagga
     3841 tataccgtgc caattcacga atggtcggat aatcgtacag tgtggtagcc tccagtgata
     3901 gcccctgcgt cttattaata agacgcatcc attccacgcc caatatagag tctaagccta
     3961 tatcataaaa gttttgatcc aaatccaccg tttccccttc aataaataag gtctgaacca
     4021 gactatcgat caacatgtct tgaagtgctg cctcatcttc ttccgcataa tgagacacct
     4081 gatggtaatc aataggctgg tcaatcttgt ataaagtgac tggtgataat acttgctctt
     4141 gatggtgatc cggccaatcc ggcatgttct tcaatgctct taattgaatc tgatccggct
     4201 ttaacgtctg ttgttcaacc tcttgcaagc taactgtctg tttagtttta gatggtagac
     4261 tggccaccgg ctctggcatc tgcgatggac taactttaac gttttcaatc aatgtcttat
     4321 cgtctttttt cggccaaaaa cttttacgtt caaagggata cgtaggcaaa ttaatacggt
     4381 gtggcagatg gtctccatac aacagtttcc agtcaacctc gccgccttgt atccaccaaa
     4441 gggctgcctc gtgccattgt ccttcttcaa aagtcttgtt aagacgcttg tctggcttct
     4501 tgttttccct ggttaaggtg cctgtcacca ggttggacgg ttgttgacct aagcgataca
     4561 ttctaaaggt ttgcagcagc tcttccgatg aatgaaccaa gaacgccatc cggcactcca
     4621 tcggctcccg tccaacctgc aaggtatagg ccatctgttg caggtcgatg gcgggattct
     4681 tttggataaa atggatcatc cgatcggcat agtgtttcag ttgtttgtcg tttttggctg
     4741 aaagcaccat taatactggt tgatccacat gataattgat ggcagtatcc gattcttcca
     4801 gtggtaaata ctcttccacc accatatgag cattggttcc actgaagcca aaggaactga
     4861 cggcggcact gcgtggcttt ccctgacgcg ttgtccaagg ctctttgttt ttgctgataa
     4921 aaaagggact atctgcaagg tctatgtgct catttaactg atcatagtgg atcgttggtg
     4981 gcagagtccg gtgtttaata gccaaaagca ctttaatcaa tcccgcaatc cctgctgctg
     5041 tcagcaaatg cccgatatta cttttaacag agcccagtgc gcaatactgt ttttcagtag
     5101 taaaagattg gaaggcttct gtcagtgctt ccacttcaat gggatcaccc aatttggttc
     5161 ccgttccatg ggcctcaacc agtgatatat cagctggatc gattccaaat ttttcataaa
     5221 cggatttttc cagatctgtt tgtgcttgcc cattaggtgc cgttataccg ttggtttttc
     5281 catcctgatt gacaccccaa ccctttatga caccatagat cgtatcctta tctcgaatag
     5341 catcgctaag acgttttaat aggaccacac ccactccttc gccaggaaca aatccatccg
     5401 cttgttgatc aaatgtccgg caacgcccct gttgggacag catccccgcc ttactggtca
     5461 ttatatggag agacggacct gccatcatac aaacccctcc tgcaagggcc aaatcactat
     5521 tgcccaatac gaggctgtta caagcctctg caaccgctac caatgaagat gaacatgcag
     5581 tttcaatcgc caaggatggg ccagataaat tgagcagata agaaattcgg gctgccagaa
     5641 tagatgtcgt gttgcccatg agcatacggg aatttaagtt ttgttcatta aataattgcc
     5701 cataatcact ggttgcacat ccaacaaata caccacattg gcttcctgaa aggtcagatg
     5761 gccgaatgcc tgcatcctct atacaactcc agcaattctc gagaaacatc ctttgctgtg
     5821 gatccattgc gatggcgtcc actggtggga tattaaagaa attcggatca aatttgtcca
     5881 catcctctaa aactcccatc catttgctag tactcttccc cggaacttct ggatcggtat
     5941 cgtagtagtg tgccattggc catcgggaag gtggaatctc tgaaatacaa tctttcccac
     6001 tgataatatt ttcccaaaat gcgttgatat tatcagcttt aggaaactgc ccagatatac
     6061 ccacaatcgc aatatccgtc tctttaagac gcggatcctc aatagctcgt tgctggattg
     6121 acggtgtctc gacagcaaca gctcgagggc tatatcgggt ttgcaggtgt tcgcaagcct
     6181 gctccatcag caagacggcg acatctacca catgacgctt tttccagtct tcgtatggcg
     6241 tgccctttac ccattgatta aacgccccta aggcgggacc acaaaatatt tgaaagttat
     6301 ctttttcagt taaatcgccg ttaagtgtgg cccggttaca ataggcgaaa taccatttaa
     6361 aaataagcgc catcttaaag cgaggattga cactcgcttc tgttaattgc tgagggtttt
     6421 tctttttctt gtattcgcaa actaaatccc agacttcggc gaaggtgcgc ttaaaataat
     6481 tgatttcaat ttcctgttta atctcttgtg gtatttcttc gatggcatta tactgcataa
     6541 acagctcata cagcctatta gcccggttat aaaactgggt atgtttacgg accacttggg
     6601 ctttcgcacc aatctcaaac atatcacctg caactgtcac agccgtatca tgaatggaaa
     6661 tggtgctaag caactctttt attacatcgt gtgcgccact ttcaaccgta cactggttaa
     6721 tggagccagt caaaacaaag tcagctccca tcataaaggc tgatgccacc gcctctggag
     6781 taccaatccc tccaccgcat cctacaagga tgggttccgg atatgagtag gtttgctgta
     6841 tatcctttcg cagggctagc acagaaggga caagagcaga cagcacgcct cgatcggtat
     6901 gaccgcctga gtccgcctct aaaacaatat catcaaccat tggaatagat cgggataata
     6961 ccgcctcttc tgctgtgatc aagccttctt ccaacagttg atccactaaa tcctctggcg
     7021 gaggcgccat aaattgtttg gctatctcca aacgagagca ttttcctatg attcttcttg
     7081 gaaaatagag gatgccatcc ttctgctttt taatcccttt gacacggcaa taaaccaatg
     7141 gtgccgttat gcttgaaaag gcagatgctt caataaaggg aacgccatgg gttataaata
     7201 tctgggcttg ctcccactct tcttccaggt tattcacatt ggcgattagg cacatgccat
     7261 aaggcttatt gggtcctaca acagattgaa tatgttggat atgtgacgcc aacctgtctc
     7321 catccaagcc ggcgctgcca aaaaagctaa gcaagttagc ttctgccatg gcgatcacca
     7381 atgcttctga agcgacccct tgagccattg aacccgccat atagttccat ttacaacggt
     7441 agcgggtttg aaacaatttg tctcccaggc tttccggacg gatcacattc ttctttactc
     7501 ctggacttgc cggcttctga ttaagcaatg cagggttctt attcccatga atggaagtgg
     7561 gagacgcatc tattttcata atcggtgcct ttaaagtctc tttcgtatcc ccttttcctt
     7621 cagctgacaa aggtttctgt ttatttggtc gtttttttaa acggcccaac tcactcgcaa
     7681 catattgtgt aaagtcaagt agtgtcggat aatcatacac cttggtggct ttaatggcta
     7741 ttccataggt tttatttaag gtcttaatcc actcaactcc aacgatagaa tctaggccca
     7801 attcaataaa ttttttacct ggcagaatca tctcacgctc aatatataaa gcttctgcta
     7861 aactgtccgc caattcttcc tgcaattctt ccaacccctg ctcctcgtaa gactcctctt
     7921 ctatgctatc ttgtttagaa tctggcaaca ccatctcaac aggagatgaa gcggggatac
     7981 ttatggtttt atcatttgcg acaacagata atgcggttgc aatttcaggc tgtgaagaat
     8041 cgactacagg tgtctgttcc ggttctgacg ctttgggtgc ccaataggaa ttcctcgcaa
     8101 acggataagt gggcaaatga acccgttcag gctcttcctt ccacatttgt ttccaaggta
     8161 aatcgtagcc ttggcagtaa aggttagaca gaccttgcaa aatttcctga tattgctctg
     8221 atgttgccgc attatggctc tgctgaatga ggtcacttcc aaacaactcc atgacctttt
     8281 gactcataaa tccttctgat acctcaccgc tatagctatt tggcaagtgc tccccgcctt
     8341 taaactgttt cagcaggtat aaagcttccg tctggtcttt gaccaccact acatatcgat
     8401 gacgaaaatg ctggcg
//
