LOCUS       NZ_JALAVA010000014      2702 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain EC30O5B-F2-1 contig_0000014, whole
            genome shotgun sequence.
ACCESSION   NZ_JALAVA010000014
VERSION     NZ_JALAVA010000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 429.34545439822875x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:33:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,521
            CDSs (total)                      :: 4,448
            Genes (coding)                    :: 4,297
            CDSs (with protein)               :: 4,297
            Genes (RNA)                       :: 73
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 63
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 84 of 151
            Pseudo Genes (incomplete)         :: 86 of 151
            Pseudo Genes (internal stop)      :: 28 of 151
            Pseudo Genes (multiple problems)  :: 40 of 151
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:09:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2702
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAVA010000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     cand_cluster    1..2702
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(ATd and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /kind="single"
                     /product="transAT-PKS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2702
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:2698](-)"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2698)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
     aSDomain        complement(<1..611)
                     /aSDomain="PKS_DH2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22440_PKS_DH2.1"
                     /evalue="7.40E-61"
                     /label="MOE81_RS22440_PKS_DH2.1"
                     /locus_tag="MOE81_RS22440"
                     /protein_end="899"
                     /protein_start="695"
                     /score="197.4"
                     /tool="antismash"
                     /translation="FTGEEPFLSDHVVGGQRMLPGSAYLEMARAAVSQAVGGWLGEGEG
                     IRLQQVGWVRPLIVGDEPVEIHMGLAVEKDETIRFDVYRETDEGEEVVYSEGRAELLTE
                     KAPPLVDLSRIQQACGQKELSREAVYTAFANMGIQYGESHRGIERIYVGQEEVLARLTL
                     ASPWVHGGLHPGMVDAAIQAGVGLQEGFDQRQPSVPFAMEE"
     aSModule        complement(<1..2483)
                     /domains="nrpspksdomains_MOE81_RS22440_PKS_KS.1"
                     /domains="nrpspksdomains_MOE81_RS22440_Trans-AT_docking.1"
                     /domains="nrpspksdomains_MOE81_RS22440_PKS_DH2.1"
                     /incomplete
                     /locus_tags="MOE81_RS22440"
                     /tool="antismash"
                     /type="pks"
     CDS             complement(<1..2698)
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (71-493). E-value:
                     5e-170. Score: 557.9. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22440_PKS_KS.1"
                     /NRPS_PKS="Domain: Trans-AT_docking (508-662). E-value:
                     1.6e-57. Score: 186.2. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22440_Trans-AT_docking.1"
                     /NRPS_PKS="Domain: PKS_DH2 (695-899). E-value: 7.4e-61.
                     Score: 197.4. Matches aSDomain:
                     nrpspksdomains_MOE81_RS22440_PKS_DH2.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: ATd"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: tra_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716798.1"
                     /locus_tag="MOE81_RS22440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE81_22390"
                     /product="type I polyketide synthase"
                     /protein_id="WP_268397741.1"
                     /sec_met_domain="tra_KS (E-value: 2.6e-196, bitscore:
                     638.6, seeds: 44, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.4e-87, bitscore:
                     277.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="ATd (E-value: 2.6e-59, bitscore: 186.2,
                     seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLFFEYKTVDELTGYFLQHHREALTKVLGDSQPAPVSQPVGTEK
                     RHKHSRRRRKEFQKAPSFSSSSSTPDIAIVGLAGRYPGARNMQEFWANLKGGKDCITPV
                     PEERLALWQIPQKGEMWGGFLQDVDRFDPLFFHISPREAERMDPQERLFLECVYETLQD
                     AGYTRQSLGAIQGQGMAGNVGVFVGVMYEEYQLYGAQEQLKGRPVAVNGNAASIANRVS
                     YFCNFHGPSMAVDTMCSSSLTAIHLACQHLQRGSCEAAIAGGVNISIHPNKYVALNQGE
                     FLSSKGRCESFGEGGDGYVPGEGVGAVLLKPLTQAVADGDHIYGVIKGTAINHGGKTHG
                     YTVPNPTAQTDVIQRALQQAKVDPRTIDYIEAHGTGTALGDPIEIAGLTKAFEAYTQDR
                     QFCAIGSVKSNIGHAESAAGIAGISKILLQMKYGQLVPSLHSKVLNTHIDFASTPFAVQ
                     QTLAEWERPPEGPRRAGISSFGAGGANAHVVIEEYIPEAAQETIAVFSPGPYLIVLSAQ
                     SEGQLLEQAKRLLSAVTEAGWTDRDLADIAYTLQIGREAMDERLAVVVTSIADLKAKLT
                     DIIAQTPDMTDYYRGEVKAHKAAMAVLVDEDMAVTLEAWQQKGKYDKLLKLWVKGMAVD
                     WERLYSETKPRRISLPTYPFTRDRYWIPVEEPKGQGITEAFHPLLHQNTSDFYEQRFST
                     RFTGEEPFLSDHVVGGQRMLPGSAYLEMARAAVSQAVGGWLGEGEGIRLQQVGWVRPLI
                     VGDEPVEIHMGLAVEKDETIRFDVYRETDEGEEVVYSEGRAELLTEKAPPLVDLSRIQQ
                     ACGQKELSREAVYTAFANMGIQYGESHRGIERIYVGQEEVLARLTLASPWVHGGLHPGM
                     VDAAIQAGVGLQEGFDQRQPSVPFAMEEV"
     source          1..2702
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="EC30O5B-F2-1"
                     /submitter_seqid="contig_0000014"
     gene            complement(<1..>2702)
                     /locus_tag="MOE81_RS22440"
                     /old_locus_tag="MOE81_22390"
     region          1..2702
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="transAT-PKS-like"
                     /region_number="1"
                     /rules="cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS
                     or itr_KS or tra_KS) and not PKS_AT)"
                     /tool="antismash"
     CDS_motif       complement(516..593)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22440_0004"
                     /evalue="8.10E-12"
                     /label="PKSI-DH_m2"
                     /locus_tag="MOE81_RS22440"
                     /protein_end="727"
                     /protein_start="701"
                     /score="37.5"
                     /tool="antismash"
                     /translation="FLSDHVVGGQRMLPGSAYLEMARAAV"
     aSDomain        complement(711..1172)
                     /aSDomain="Trans-AT_docking"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22440_Trans-AT_docking.1
                     "
                     /evalue="1.60E-57"
                     /label="MOE81_RS22440_Trans-AT_docking.1"
                     /locus_tag="MOE81_RS22440"
                     /protein_end="662"
                     /protein_start="508"
                     /score="186.2"
                     /tool="antismash"
                     /translation="PYLIVLSAQSEGQLLEQAKRLLSAVTEAGWTDRDLADIAYTLQIG
                     REAMDERLAVVVTSIADLKAKLTDIIAQTPDMTDYYRGEVKAHKAAMAVLVDEDMAVTL
                     EAWQQKGKYDKLLKLWVKGMAVDWERLYSETKPRRISLPTYPFTRDRYWI"
     aSDomain        complement(1218..2483)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE81_RS22440_PKS_KS.1"
                     /domain_subtypes="Trans-AT-KS"
                     /domain_subtypes="keto"
                     /evalue="5.00E-170"
                     /label="MOE81_RS22440_PKS_KS.1"
                     /locus_tag="MOE81_RS22440"
                     /protein_end="493"
                     /protein_start="71"
                     /score="557.9"
                     /specificity="transATor: keto"
                     /tool="antismash"
                     /translation="IAIVGLAGRYPGARNMQEFWANLKGGKDCITPVPEERLALWQIPQ
                     KGEMWGGFLQDVDRFDPLFFHISPREAERMDPQERLFLECVYETLQDAGYTRQSLGAIQ
                     GQGMAGNVGVFVGVMYEEYQLYGAQEQLKGRPVAVNGNAASIANRVSYFCNFHGPSMAV
                     DTMCSSSLTAIHLACQHLQRGSCEAAIAGGVNISIHPNKYVALNQGEFLSSKGRCESFG
                     EGGDGYVPGEGVGAVLLKPLTQAVADGDHIYGVIKGTAINHGGKTHGYTVPNPTAQTDV
                     IQRALQQAKVDPRTIDYIEAHGTGTALGDPIEIAGLTKAFEAYTQDRQFCAIGSVKSNI
                     GHAESAAGIAGISKILLQMKYGQLVPSLHSKVLNTHIDFASTPFAVQQTLAEWERPPEG
                     PRRAGISSFGAGGANAHVVIEEY"
     CDS_motif       complement(1440..1484)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22440_0003"
                     /evalue="3.30E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="MOE81_RS22440"
                     /protein_end="419"
                     /protein_start="404"
                     /score="12.9"
                     /tool="antismash"
                     /translation="GSVKSNIGHAESAAG"
     CDS_motif       complement(1545..1592)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22440_0002"
                     /evalue="6.90E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="MOE81_RS22440"
                     /protein_end="384"
                     /protein_start="368"
                     /score="14.6"
                     /tool="antismash"
                     /translation="YIEAHGTGTALGDPIE"
     CDS_motif       complement(1965..2012)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE81_RS22440_0001"
                     /evalue="1.70E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="MOE81_RS22440"
                     /protein_end="244"
                     /protein_start="228"
                     /score="20.0"
                     /tool="antismash"
                     /translation="GPSMAVDTMCSSSLTA"
ORIGIN
        1 cacttcctcc atcgcaaagg gcacagatgg ttgacgctgg tcgaagccct cctgcaaccc
       61 tacccccgct tgtatggctg catccaccat tcccggatga agcccgccat gcacccaagg
      121 ggatgcgagc gtgagccggg ccaggacctc ttcctgcccc acatagatcc gttcaatccc
      181 ccgatggctt tcgccatatt gaatgcccat attggcaaag gccgtataaa ccgcttccct
      241 gctaagctct ttttgtccac aggcctgctg tattcgggaa agatccacta gcggcggggc
      301 tttctctgtc agcagctcag cccgcccctc gctatatacc acttcttcgc cttcatccgt
      361 ttcccggtag acgtcaaagc ggatggtctc atccttttca accgctaacc ccatatggat
      421 ctcaaccggt tcatcaccga caataagggg gcgcacccag cctacctgct ggagacggat
      481 tccttctcct tcacccagcc atccgccaac agcctgggag acagccgctc gcgccatttc
      541 cagataggcg cttcccggta acattcgctg tcctcccaca acgtgatcgg atagaaaagg
      601 ttcttccccc gtgaagcggg tgctaaaccg ctgttcatag aagtctgatg tattctggtg
      661 caataaggga tgaaacgcct ctgttattcc ctgtcctttc ggctcttcta ctggaatcca
      721 ataccggtcc cgtgtaaagg gataagttgg caagctgatc cgccgcggct tggtctcgct
      781 gtacaaccgt tcccaatcga cagccatccc cttaacccac agctttaata gcttgtcata
      841 cttacctttt tgctgccatg cctccagtgt gacggccata tcctcgtcca ctaggacagc
      901 catggccgct ttatgggctt tcacctcacc ccgataataa tctgtcatat caggcgtttg
      961 ggcgataata tccgtcagct ttgccttgag atcggcaatg gaggtcacca ccactgccaa
     1021 tcgctcatcc attgcttccc ttccgatctg caaggtatag gcaatatccg ccagatctcg
     1081 atctgtccag cccgcttcgg ttactgctga caacagccgc ttggcctgct ccaacagttg
     1141 tccttcgctt tgggccgata ggacaatcag gtaaggaccc ggagaaaaaa cggcaatcgt
     1201 ctcttgtgcc gcttcgggta tatattcctc aatcaccaca tgggcattgg ctccaccggc
     1261 accgaaggag gagatacctg cccgccgcgg cccctccggg ggccgttccc attcagctaa
     1321 tgtctgttgc accgcaaagg gggtggaggc aaaatcaata tgtgtattta agaccttgga
     1381 atgaagggat ggcaccagtt gcccatattt catctgaagc aggattttac ttatgcccgc
     1441 aatccctgca gcactctctg catgcccgat attggatttc accgaaccga tggcacaaaa
     1501 ctgccgatct tgagtataag cctcaaaggc tttggttaat ccggcaattt caatgggatc
     1561 accaagggct gtgcctgtgc catgagcttc gatgtagtcg atggtccgcg ggtcgacttt
     1621 tgcctgttgc aatgcacgtt ggatcacatc cgtctgggca gtggggttag gaacggtata
     1681 gccgtgggtt ttgcctccat ggttaatcgc tgttcctttg atcactccat aaatatgatc
     1741 cccatcggcc acagcctggg ttaagggctt taacagaact gcccccaccc cttcaccggg
     1801 tacgtagccg tctcctcctt cgccaaagct ttcacaacgt cccttgctcg ataaaaactc
     1861 cccctgattt aaggcgacat atttattagg gtgaatggag atattcaccc cgccggcaat
     1921 ggccgcctca cagctgcccc gctgcaggtg ctgacaggct aaatggatgg ccgttaagga
     1981 agaggagcac atggtatcca cagccatact tggaccatgg aagttacaaa aataggagac
     2041 ccggttggct atagaggctg cattgccatt gactgccacc ggccggccct taagctgttc
     2101 ctgagcccca tacaactggt actcctcata catcacaccg acaaacactc caacattgcc
     2161 agccatcccc tgcccctgaa tggcacctaa cgactggcgg gtatatcccg catcctggag
     2221 ggtttcatag acgcattcca aaaagagccg ttcctgcgga tccatccgtt ctgcttcccg
     2281 aggtgagata tgaaaaaaca ggggatcaaa tcgatccaca tcctgtaaaa aaccacccca
     2341 catctctcct ttttgaggaa tttgccaaag ggccaggcgt tcctcaggaa ccggggtgat
     2401 acaatctttt ccacctttta agttggccca aaactcctgc atattccggg caccgggata
     2461 acgccccgcc aaaccgacaa tggcaatatc gggggtggat gaggatgaag agaaggaagg
     2521 cgccttctga aattctttcc gccgcctgcg cgaatgcttg tgccgctttt ccgtgcccac
     2581 cggttgagag acaggcgcag gctggctgtc gccaaggacc tttgtcaatg cctcacgatg
     2641 gtgctggaga aaatatcccg ttagctcgtc caccgttttg tattcaaaga acagggtttt
     2701 ag
//
