LOCUS       NZ_JALAUV010000015      2638 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain EC49O2B-D16 contig_0000015, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAUV010000015
VERSION     NZ_JALAUV010000015.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 346.2863018625785x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:36:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,571
            CDSs (total)                      :: 4,491
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 86 of 144
            Pseudo Genes (incomplete)         :: 78 of 144
            Pseudo Genes (internal stop)      :: 27 of 144
            Pseudo Genes (multiple problems)  :: 40 of 144
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:50:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2638
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAUV010000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..2636)
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (71-493). E-value:
                     4.7e-170. Score: 558.0. Matches aSDomain:
                     nrpspksdomains_MOE71_RS19470_PKS_KS.1"
                     /NRPS_PKS="Domain: Trans-AT_docking (508-662). E-value:
                     1.5e-57. Score: 186.3. Matches aSDomain:
                     nrpspksdomains_MOE71_RS19470_Trans-AT_docking.1"
                     /NRPS_PKS="Domain: PKS_DH2 (695-878). E-value: 2.9e-58.
                     Score: 189.0. Matches aSDomain:
                     nrpspksdomains_MOE71_RS19470_PKS_DH2.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: tra_KS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: ATd"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015892240.1"
                     /locus_tag="MOE71_RS19470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE71_19430"
                     /product="type I polyketide synthase"
                     /protein_id="WP_268426352.1"
                     /sec_met_domain="tra_KS (E-value: 2.4e-196, bitscore:
                     638.7, seeds: 44, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.2e-87, bitscore:
                     277.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="ATd (E-value: 2.4e-59, bitscore: 186.3,
                     seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLFFEYKTVDELTGYFLQHHREALTKVLGDSQPAPVSQPVGTEK
                     RHKHSRRRRKEFQKAPSFSSSSSTPDIAIVGLAGRYPGARNMQEFWANLKGGKDCITPV
                     PEERLALWQIPQKGEMWGGFLQDVDRFDPLFFHISPREAERMDPQERLFLECVYETLQD
                     AGYTRQSLGAIQGQGMAGNVGVFVGVMYEEYQLYGAQEQLKGRPVAVNGNAASIANRVS
                     YFCNFHGPSMAVDTMCSSSLTAIHLACQHLQRGSCEAAIAGGVNISIHPNKYVALNQGE
                     FLSSKGRCESFGEGGDGYVPGEGVGAVLLKPLTQAVADGDHIYGVIKGTAINHGGKTHG
                     YTVPNPTAQTDVIQRALQQAKVDPRTIDYIEAHGTGTALGDPIEIAGLTKAFEAYTQDR
                     QFCAIGSVKSNIGHAESAAGIAGISKILLQMKYGQLVPSLHSKVLNTHIDFASTPFAVQ
                     QTLAEWERPPEGPRRAGISSFGAGGANAHVVIEEYIPEAAQETIAVFSPGPYLIVLSAQ
                     SEGQLLEQAKRLLSVVTEAGWTDRDLADIAYTLQIGREAMDERLAVVVTSIADLKAKLT
                     DIIAQTPDMTDYYRGEVKAHKAAMAVLVDEDMAVTLEAWQQKGKYDKLLKLWVKGMAVD
                     WERLYSETKPRRISLPTYPFTRDRYWIPVEEPKGQGITEAFHPLLHQNTSDFYEQRFST
                     RFTGEEPFLSDHVVGGQRMLPGSAYLEMARAAVSQAVGGWLGEGEGIRLQQVGWVRPLI
                     VGDEPVEIHMGLAVEKDETIRFDVYRETDEGEEVVYSEGRAELLTEKAPPLVDLSRIQQ
                     ACGQKELSREAVYTAFANMGIQYGESHRGIERIYVGQEEVLARLTLASPWVHGGLHPGM
                     VDAAIQAG"
     source          1..2638
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="EC49O2B-D16"
                     /submitter_seqid="contig_0000015"
     gene            complement(<1..>2638)
                     /locus_tag="MOE71_RS19470"
                     /old_locus_tag="MOE71_19430"
     region          1..2638
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="transAT-PKS-like"
                     /region_number="1"
                     /rules="cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS
                     or itr_KS or tra_KS) and not PKS_AT)"
                     /tool="antismash"
     cand_cluster    1..2638
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(ATd and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /kind="single"
                     /product="transAT-PKS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2638
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:2636](-)"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2636)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
     aSDomain        complement(2..550)
                     /aSDomain="PKS_DH2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS19470_PKS_DH2.1"
                     /evalue="2.90E-58"
                     /label="MOE71_RS19470_PKS_DH2.1"
                     /locus_tag="MOE71_RS19470"
                     /protein_end="878"
                     /protein_start="695"
                     /score="189.0"
                     /tool="antismash"
                     /translation="FTGEEPFLSDHVVGGQRMLPGSAYLEMARAAVSQAVGGWLGEGEG
                     IRLQQVGWVRPLIVGDEPVEIHMGLAVEKDETIRFDVYRETDEGEEVVYSEGRAELLTE
                     KAPPLVDLSRIQQACGQKELSREAVYTAFANMGIQYGESHRGIERIYVGQEEVLARLTL
                     ASPWVHGGLHPGMVDAAIQA"
     aSModule        complement(2..2422)
                     /domains="nrpspksdomains_MOE71_RS19470_PKS_KS.1"
                     /domains="nrpspksdomains_MOE71_RS19470_Trans-AT_docking.1"
                     /domains="nrpspksdomains_MOE71_RS19470_PKS_DH2.1"
                     /incomplete
                     /locus_tags="MOE71_RS19470"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(455..532)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS19470_0004"
                     /evalue="7.90E-12"
                     /label="PKSI-DH_m2"
                     /locus_tag="MOE71_RS19470"
                     /protein_end="727"
                     /protein_start="701"
                     /score="37.5"
                     /tool="antismash"
                     /translation="FLSDHVVGGQRMLPGSAYLEMARAAV"
     aSDomain        complement(650..1111)
                     /aSDomain="Trans-AT_docking"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS19470_Trans-AT_docking.1
                     "
                     /evalue="1.50E-57"
                     /label="MOE71_RS19470_Trans-AT_docking.1"
                     /locus_tag="MOE71_RS19470"
                     /protein_end="662"
                     /protein_start="508"
                     /score="186.3"
                     /tool="antismash"
                     /translation="PYLIVLSAQSEGQLLEQAKRLLSVVTEAGWTDRDLADIAYTLQIG
                     REAMDERLAVVVTSIADLKAKLTDIIAQTPDMTDYYRGEVKAHKAAMAVLVDEDMAVTL
                     EAWQQKGKYDKLLKLWVKGMAVDWERLYSETKPRRISLPTYPFTRDRYWI"
     aSDomain        complement(1157..2422)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS19470_PKS_KS.1"
                     /domain_subtypes="Trans-AT-KS"
                     /domain_subtypes="keto"
                     /evalue="4.70E-170"
                     /label="MOE71_RS19470_PKS_KS.1"
                     /locus_tag="MOE71_RS19470"
                     /protein_end="493"
                     /protein_start="71"
                     /score="558.0"
                     /specificity="transATor: keto"
                     /tool="antismash"
                     /translation="IAIVGLAGRYPGARNMQEFWANLKGGKDCITPVPEERLALWQIPQ
                     KGEMWGGFLQDVDRFDPLFFHISPREAERMDPQERLFLECVYETLQDAGYTRQSLGAIQ
                     GQGMAGNVGVFVGVMYEEYQLYGAQEQLKGRPVAVNGNAASIANRVSYFCNFHGPSMAV
                     DTMCSSSLTAIHLACQHLQRGSCEAAIAGGVNISIHPNKYVALNQGEFLSSKGRCESFG
                     EGGDGYVPGEGVGAVLLKPLTQAVADGDHIYGVIKGTAINHGGKTHGYTVPNPTAQTDV
                     IQRALQQAKVDPRTIDYIEAHGTGTALGDPIEIAGLTKAFEAYTQDRQFCAIGSVKSNI
                     GHAESAAGIAGISKILLQMKYGQLVPSLHSKVLNTHIDFASTPFAVQQTLAEWERPPEG
                     PRRAGISSFGAGGANAHVVIEEY"
     CDS_motif       complement(1379..1423)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS19470_0003"
                     /evalue="3.20E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="MOE71_RS19470"
                     /protein_end="419"
                     /protein_start="404"
                     /score="13.0"
                     /tool="antismash"
                     /translation="GSVKSNIGHAESAAG"
     CDS_motif       complement(1484..1531)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS19470_0002"
                     /evalue="6.70E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="MOE71_RS19470"
                     /protein_end="384"
                     /protein_start="368"
                     /score="14.7"
                     /tool="antismash"
                     /translation="YIEAHGTGTALGDPIE"
     CDS_motif       complement(1904..1951)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS19470_0001"
                     /evalue="1.60E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="MOE71_RS19470"
                     /protein_end="244"
                     /protein_start="228"
                     /score="20.0"
                     /tool="antismash"
                     /translation="GPSMAVDTMCSSSLTA"
ORIGIN
        1 ccccgcttgt atggctgcat ccaccattcc cggatgaagc ccgccatgca cccaagggga
       61 tgcgagcgtg agccgggcca ggacctcttc ctgccccaca tagatccgtt caatcccccg
      121 atggctttcg ccatattgaa tgcccatatt ggcaaaggcc gtataaaccg cttccctgct
      181 aagctctttt tgtccacagg cctgctgtat tcgggaaaga tccactagcg gcggggcttt
      241 ctctgtcagc agctcagccc gcccctcgct atataccact tcttcgcctt catccgtttc
      301 ccggtagacg tcaaagcgga tggtctcatc cttttcaacc gctaacccca tatggatctc
      361 aaccggttca tcaccgacaa taagggggcg cacccagcct acctgctgga gacggattcc
      421 ttctccttca cccagccatc cgccaacagc ctgggagaca gccgctcgcg ccatttccag
      481 ataggcgctt cccggtaaca ttcgctgtcc tcccacaacg tgatcggata gaaaaggttc
      541 ttcccccgtg aagcgggtgc taaaccgctg ttcatagaag tctgatgtat tctggtgcaa
      601 taagggatga aacgcctctg ttattccctg tcctttcggc tcttctactg gaatccaata
      661 ccggtcccgt gtaaagggat aagttggcaa gctgatccgc cgcggcttgg tctcgctgta
      721 caaccgttcc caatcgacag ccatcccctt aacccacagc tttaatagct tgtcatactt
      781 acctttttgc tgccatgcct ccagtgtgac ggccatatcc tcgtccacta ggacagccat
      841 ggccgcttta tgggctttca cctcaccccg ataataatct gtcatatcag gcgtttgggc
      901 gataatatcc gtcagctttg ccttgagatc ggcaatggag gtcaccacca ctgccaatcg
      961 ctcatccatt gcttcccttc cgatctgcaa ggtataggca atatccgcca gatctcgatc
     1021 tgtccagccc gcttcggtta ctactgacaa cagccgcttg gcctgctcca acagttgtcc
     1081 ttcgctttgg gccgatagga caatcaggta aggacccgga gaaaaaacgg caatcgtctc
     1141 ttgtgccgct tcgggtatat attcctcaat caccacatgg gcattggctc caccggcacc
     1201 gaaggaggag atacctgccc gccgcggccc ctccgggggc cgttcccatt cagctaatgt
     1261 ctgttgcacc gcaaaggggg tggaggcaaa atcaatatgt gtatttaaga ccttggaatg
     1321 aagggatggc accagttgcc catatttcat ctgaagcagg attttactta tgcccgcaat
     1381 ccctgcagca ctctctgcat gcccgatatt ggatttcacc gaaccgatgg cacaaaactg
     1441 ccgatcttga gtataagcct caaaggcttt ggttaatccg gcaatttcaa tgggatcacc
     1501 aagggctgtg cctgtgccat gagcttcgat gtagtcgatg gtccgcgggt cgacttttgc
     1561 ctgttgcaat gcacgttgga tcacatccgt ctgggcagtg gggttaggaa cggtatagcc
     1621 gtgggttttg cctccatggt taatcgctgt tcctttgatc actccataaa tatgatcccc
     1681 atcggccaca gcctgggtta agggctttaa cagaactgcc cccacccctt caccgggtac
     1741 atagccgtct cctccttcgc caaagctttc acaacgtccc ttgctcgata aaaactcccc
     1801 ctgatttaag gcgacatatt tattagggtg aatggagata ttcaccccgc cggcaatggc
     1861 cgcctcacag ctgccccgct gcaggtgctg acaggctaaa tggatggccg ttaaggaaga
     1921 ggagcacatg gtatccacag ccatacttgg accatggaag ttacaaaaat aggagacccg
     1981 gttggctata gaggctgcat tgccattgac tgccaccggc cggcccttaa gctgttcctg
     2041 agccccatac aactggtact cctcatacat cacaccgaca aacactccaa cattgccagc
     2101 catcccctgc ccctgaatgg cacctaacga ctggcgggta tatcccgcat cctggagggt
     2161 ttcatagacg cattccaaaa agagccgttc ctgcggatcc atccgttctg cttcccgagg
     2221 tgagatatga aaaaacaggg gatcaaatcg atccacatcc tgtaaaaaac caccccacat
     2281 ctctcctttt tgaggaattt gccaaagggc caggcgttcc tcaggaaccg gggtgataca
     2341 atcttttcca ccttttaagt tggcccaaaa ctcctgcata ttccgggcac cgggataacg
     2401 ccccgccaaa ccgacaatgg caatatcggg ggtggatgag gatgaagaga aggaaggcgc
     2461 cttctgaaat tctttccgcc gcctgcgcga atgcttgtgc cgcttttccg tgcccaccgg
     2521 ttgagagaca ggcgcaggct ggctgtcgcc aaggaccttt gtcaatgcct cacgatggtg
     2581 ctggagaaaa tatcccgtta gctcgtccac cgttttgtat tcaaagaaca gggtttta
//
