LOCUS       NZ_JALAUV010000012      8414 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus inaquosorum strain EC49O2B-D16 contig_0000012, whole genome
            shotgun sequence.
ACCESSION   NZ_JALAUV010000012
VERSION     NZ_JALAUV010000012.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus inaquosorum
  ORGANISM  Bacillus inaquosorum
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Dunlap,C.
  TITLE     Crop Bioprotection Bacillus Genome Sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Dunlap,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2022) CBP, USDA-ARS-NCAUR, 1815 N. University St.,
            Peoria, IL 61604, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 346.2863018625785x
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 02:36:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,571
            CDSs (total)                      :: 4,491
            Genes (coding)                    :: 4,347
            CDSs (with protein)               :: 4,347
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 68
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 86 of 144
            Pseudo Genes (incomplete)         :: 78 of 144
            Pseudo Genes (internal stop)      :: 27 of 144
            Pseudo Genes (multiple problems)  :: 40 of 144
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:50:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8414
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JALAUV010000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..8414
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<1544:8325](-)"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1545..8325)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="45000"
                     /detection_rule="cds(ATd and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /neighbourhood="20000"
                     /product="transAT-PKS-like"
                     /protocluster_number="1"
     gene            complement(<1..547)
                     /locus_tag="MOE71_RS18150"
                     /old_locus_tag="MOE71_18110"
     CDS             complement(<1..547)
                     /NRPS_PKS="Domain: PP-binding (44-110). E-value: 7.2e-15.
                     Score: 47.0. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18150_PP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (128-181). E-value: 4.4e-11.
                     Score: 34.9. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18150_PP-binding.2"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /locus_tag="MOE71_RS18150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE71_18110"
                     /product="acyl carrier protein"
                     /protein_id="WP_268291526.1"
                     /sec_met_domain="PP-binding (E-value: 2.1e-15, bitscore:
                     45.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDGQWKHIYRQLKEKEITPEEASWQFKQWGKHKEKNHAANDLLPA
                     LKEIAAEVLAIDANEIHHDVLLGEQGFDPLKLNQLANRLNTKFGLELTTKDLAEQNTLD
                     DLAQFLQAAKSSQGDQRLREKTINYLKEHLSSVFKINAEEIASDIDLEKYGINSIMVLK
                     LTQQLEGFFGPLTKTLFFE"
     source          1..8414
                     /db_xref="taxon:483913"
                     /mol_type="genomic DNA"
                     /organism="Bacillus inaquosorum"
                     /strain="EC49O2B-D16"
                     /submitter_seqid="contig_0000012"
     region          1..8414
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="transAT-PKS-like"
                     /region_number="1"
                     /rules="cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS
                     or itr_KS or tra_KS) and not PKS_AT)"
                     /tool="antismash"
     cand_cluster    1..8414
                     /SMILES="CC(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(ATd and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS) and not PKS_AT)"
                     /kind="single"
                     /product="transAT-PKS-like"
                     /protoclusters="1"
                     /tool="antismash"
     aSDomain        complement(5..163)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18150_PP-binding.2"
                     /evalue="4.40E-11"
                     /label="MOE71_RS18150_PP-binding.2"
                     /locus_tag="MOE71_RS18150"
                     /protein_end="181"
                     /protein_start="128"
                     /score="34.9"
                     /tool="antismash"
                     /translation="YLKEHLSSVFKINAEEIASDIDLEKYGINSIMVLKLTQQLEGFFG
                     PLTKTLFF"
     aSDomain        complement(218..415)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18150_PP-binding.1"
                     /evalue="7.20E-15"
                     /label="MOE71_RS18150_PP-binding.1"
                     /locus_tag="MOE71_RS18150"
                     /protein_end="110"
                     /protein_start="44"
                     /score="47.0"
                     /tool="antismash"
                     /translation="ALKEIAAEVLAIDANEIHHDVLLGEQGFDPLKLNQLANRLNTKFG
                     LELTTKDLAEQNTLDDLAQFL"
     gene            complement(561..1514)
                     /locus_tag="MOE71_RS18155"
                     /old_locus_tag="MOE71_18115"
     CDS             complement(561..1514)
                     /NRPS_PKS="Domain: PKS_DH2 (21-251). E-value: 3.9e-58.
                     Score: 188.5. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18155_PKS_DH2.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /locus_tag="MOE71_RS18155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE71_18115"
                     /product="polyketide synthase dehydratase domain-containing
                     protein"
                     /protein_id="WP_248596971.1"
                     /transl_table=11
                     /translation="MHPLVHLNTSDLSTQRFTTILTGKEAYLTDHQVKERRVLPGSAYL
                     EMARTALEQGVDRVTTDTLQQGTVFICLQEHIWIAPLVVEDKPVEVHITLALIENGNVE
                     YEIYSGSDHPDDQSAILHAQGVLKVSELTPPKRQSLQQLKSQYPWRTLLPENIYPLFRE
                     AGLYYGPSHSGIERLYLHKDQILAKISVLPESRGDRECYLDPGLLDAVFQATAGFAISL
                     NAPLDSLPFTLKEMYIYQPCPKQVWAYIRQREKGRVFDIDVYDENGMVCVRILGFETRR
                     GAGLMSEEAFYRHLSERIWNGELSQEEVERILMASR"
     aSDomain        complement(762..1451)
                     /aSDomain="PKS_DH2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18155_PKS_DH2.1"
                     /evalue="3.90E-58"
                     /label="MOE71_RS18155_PKS_DH2.1"
                     /locus_tag="MOE71_RS18155"
                     /protein_end="251"
                     /protein_start="21"
                     /score="188.5"
                     /tool="antismash"
                     /translation="TGKEAYLTDHQVKERRVLPGSAYLEMARTALEQGVDRVTTDTLQQ
                     GTVFICLQEHIWIAPLVVEDKPVEVHITLALIENGNVEYEIYSGSDHPDDQSAILHAQG
                     VLKVSELTPPKRQSLQQLKSQYPWRTLLPENIYPLFREAGLYYGPSHSGIERLYLHKDQ
                     ILAKISVLPESRGDRECYLDPGLLDAVFQATAGFAISLNAPLDSLPFTLKEMYIYQPCP
                     KQVWAYIR"
     CDS_motif       complement(1359..1436)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS18155_0001"
                     /evalue="2.50E-11"
                     /label="PKSI-DH_m2"
                     /locus_tag="MOE71_RS18155"
                     /protein_end="52"
                     /protein_start="26"
                     /score="35.9"
                     /tool="antismash"
                     /translation="YLTDHQVKERRVLPGSAYLEMARTAL"
     gene            complement(<1545..>3278)
                     /locus_tag="MOE71_RS18160"
                     /old_locus_tag="MOE71_18120"
                     /pseudo=""
     CDS             complement(<1545..3278)
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (2-422). E-value:
                     5.9e-177. Score: 580.7. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18160_PKS_KS.1"
                     /NRPS_PKS="Domain: Trans-AT_docking (438-575). E-value:
                     2.8e-29. Score: 94.4. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18160_Trans-AT_docking.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: tra_KS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: ATd"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352336.1"
                     /locus_tag="MOE71_RS18160"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="MOE71_18120"
                     /product="type I polyketide synthase"
                     /pseudo=""
                     /sec_met_domain="tra_KS (E-value: 1.1e-183, bitscore:
                     598.9, seeds: 44, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.2e-94, bitscore:
                     303.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="ATd (E-value: 1.9e-30, bitscore: 94.4,
                     seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIAIIGMSGRYPEADNLDAYWSNLKAGRDSIREIPPSRWDVASF
                     YQPFPSDEGKIYSKWLGLMDDVACFDPLFFNIAPSEAETMDPQQRLFLQEGYQAFADAG
                     YTPEALNGQNCGVYLGIMANEYYRMLCEQQYGLTDMTGNYASIASARIAYFLNLKGPAL
                     SIDTACSSSLVAINLACEALLNRKIDMALAGGVTLYLTPKPYIEMCAAHMLSPEGRCKA
                     FDKSSDGMVPGEGVGAVVLKRLQDAEADNDLIYGVIIGFGINQDGKTNGLTAPSVKSQI
                     ALERSIYQNYNIDPSSISYAEMHGTGTQLGDPIELKALSTAFQEKTDKKQFCAIGSVKS
                     NIGHTSAAAGVASVHKVLLSMQHQQLVPTLHFKEPNEYFDFKNSPFYVNTQTRPWDANS
                     PRRACVSSFGFSGTNAHLVIEAYETKKKRVRMPVTDASRPALFVLSAKTEKQLKVYAGI
                     MKEWIASQVDVNLKDMTYTLQTSREAMDYRMAFWMDSKFAAIDMLTAYLNDEQPDGLHI
                     SHVKKRKGQRNTSLHTDPETLFSQWLAGEWVDWQVFYKDDEPRHIRLPGYPFAKEHYWL
                     PK"
     aSDomain        complement(1554..1964)
                     /aSDomain="Trans-AT_docking"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18160_Trans-AT_docking.1
                     "
                     /evalue="2.80E-29"
                     /label="MOE71_RS18160_Trans-AT_docking.1"
                     /locus_tag="MOE71_RS18160"
                     /protein_end="575"
                     /protein_start="438"
                     /score="94.4"
                     /tool="antismash"
                     /translation="PALFVLSAKTEKQLKVYAGIMKEWIASQVDVNLKDMTYTLQTSRE
                     AMDYRMAFWMDSKFAAIDMLTAYLNDEQPDGLHISHVKKRKGQRNTSLHTDPETLFSQW
                     LAGEWVDWQVFYKDDEPRHIRLPGYPFAKEHYW"
     aSModule        complement(1554..3272)
                     /domains="nrpspksdomains_MOE71_RS18160_PKS_KS.1"
                     /domains="nrpspksdomains_MOE71_RS18160_Trans-AT_docking.1"
                     /incomplete
                     /locus_tags="MOE71_RS18160"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(2013..3272)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18160_PKS_KS.1"
                     /domain_subtypes="Trans-AT-KS"
                     /domain_subtypes="exomethylene"
                     /evalue="5.90E-177"
                     /label="MOE71_RS18160_PKS_KS.1"
                     /locus_tag="MOE71_RS18160"
                     /protein_end="422"
                     /protein_start="2"
                     /score="580.7"
                     /specificity="transATor: exomethylene"
                     /tool="antismash"
                     /translation="IAIIGMSGRYPEADNLDAYWSNLKAGRDSIREIPPSRWDVASFYQ
                     PFPSDEGKIYSKWLGLMDDVACFDPLFFNIAPSEAETMDPQQRLFLQEGYQAFADAGYT
                     PEALNGQNCGVYLGIMANEYYRMLCEQQYGLTDMTGNYASIASARIAYFLNLKGPALSI
                     DTACSSSLVAINLACEALLNRKIDMALAGGVTLYLTPKPYIEMCAAHMLSPEGRCKAFD
                     KSSDGMVPGEGVGAVVLKRLQDAEADNDLIYGVIIGFGINQDGKTNGLTAPSVKSQIAL
                     ERSIYQNYNIDPSSISYAEMHGTGTQLGDPIELKALSTAFQEKTDKKQFCAIGSVKSNI
                     GHTSAAAGVASVHKVLLSMQHQQLVPTLHFKEPNEYFDFKNSPFYVNTQTRPWDANSPR
                     RACVSSFGFSGTNAHLVIEAY"
     CDS_motif       complement(2229..2273)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS18160_0003"
                     /evalue="3.50E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="MOE71_RS18160"
                     /protein_end="350"
                     /protein_start="335"
                     /score="12.9"
                     /tool="antismash"
                     /translation="GSVKSNIGHTSAAAG"
     CDS_motif       complement(2334..2381)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS18160_0002"
                     /evalue="1.40E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="MOE71_RS18160"
                     /protein_end="315"
                     /protein_start="299"
                     /score="13.8"
                     /tool="antismash"
                     /translation="YAEMHGTGTQLGDPIE"
     CDS_motif       complement(2754..2801)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS18160_0001"
                     /evalue="6.20E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="MOE71_RS18160"
                     /protein_end="175"
                     /protein_start="159"
                     /score="21.2"
                     /tool="antismash"
                     /translation="GPALSIDTACSSSLVA"
     gene            complement(3394..8325)
                     /locus_tag="MOE71_RS18165"
                     /old_locus_tag="MOE71_18125"
     CDS             complement(3394..8325)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: ACP_beta (149-216). E-value: 5e-29.
                     Score: 91.9. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18165_ACP_beta.1"
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (751-1173). E-value:
                     3.4e-177. Score: 581.5. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18165_PKS_KS.1"
                     /NRPS_PKS="Domain: Trans-AT_docking (1189-1331). E-value:
                     5.1e-40. Score: 129.3. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18165_Trans-AT_docking.1"
                     /NRPS_PKS="Domain: ACP_beta (1430-1496). E-value: 2.3e-25.
                     Score: 80.2. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18165_ACP_beta.2"
                     /NRPS_PKS="Domain: ACP_beta (1526-1591). E-value: 7e-28.
                     Score: 88.2. Matches aSDomain:
                     nrpspksdomains_MOE71_RS18165_ACP_beta.3"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: tra_KS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     transAT-PKS-like: ATd"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR02814.1"
                     /locus_tag="MOE71_RS18165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="MOE71_18125"
                     /product="PfaD family polyunsaturated fatty acid/polyketide
                     biosynthesis protein"
                     /protein_id="WP_268274641.1"
                     /sec_met_domain="tra_KS (E-value: 3.2e-186, bitscore:
                     607.3, seeds: 44, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.2e-92, bitscore:
                     296.7, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="ATd (E-value: 3.4e-41, bitscore: 129.3,
                     seeds: 19, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.7e-15, bitscore:
                     45.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPNSYSGEVSEGFMSQKVMELFGSDLIQQSHNAATSEQYQEILQG
                     LSNLYCQGYDLPWKQMWKEEPERVHLPTYPFARNSYWAPKASEPEQTPVVDSSQPEIAT
                     ALSVVANDKTISIPASSPVEMVLPDSKQDSIEEESYEEQGLEELQEELADSLAEALYIE
                     REMILPGKKFIELGLDSIVGVEWIKTLNKTYGIAIKATKVYDYPTLLDFTQYVASELGR
                     LKKRPNKQKPLSAEGKGDTKETLKAPIMKIDASPTSIHGNKNPALLNQKPASPGVKKNV
                     IRPESLGDKLFQTRYRCKWNYMAGSMAQGVASEALVIAMAEANLLSFFGSAGLDGDRLA
                     SHIQHIQSVVGPNKPYGMCLIANVNNLEEEWEQAQIFITHGVPFIEASAFSSITAPLVY
                     CRVKGIKKQKDGILYFPRRIIGKCSRLEIAKQFMAPPPEDLVDQLLEEGLITAEEAVLS
                     RSIPMVDDIVLEADSGGHTDRGVLSALVPSVLALRKDIQQTYSYPEPILVGCGGGIGTP
                     EAVASAFMMGADFVLTGSINQCTVESGAHDVIKELLSTISIHDTAVTVAGDMFEIGAKA
                     QVVRKHTQFYNRANRLYELFMQYNAIEEIPQEIKQEIEINYFKRTFAEVWDLVCEYKKK
                     KNPQQLTEASVNPRFKMALIFKWYFAYCNRATLNGDLTEKDNFQIFCGPALGAFNQWVK
                     GTPYEDWKKRHVVDVAVLLMEQACEHLQTRYSPRAVAVETPSIQQRAIEDPRLKETDIA
                     IVGISGQFPKADNINAFWENIISGKDCISEIPPSRWPMAHYYDTDPEVPGKSTSKWMGV
                     LEDVDKFDPNFFNIPPVDAIAMDPQQRMFLENCWSCIEDAGIRPSDLSGSQCGVFVGCA
                     TSDYGQLFNEQNLNSRMLMGNTTSILAARISYLLNLSGPSLAIETACSSSLVAVAEACN
                     SLVLGNSDLALAGGVCMMAGPSLHIMTSKAGMLSQQGRCRTFDQQADGFVPGEGVGVVL
                     LKRLSDAIRDKDTIYGVIKGWGVNQDGKTNGITAPNGQAQTDLEKSVYEKFGIDPADIS
                     LVEAHGTGTKLGDPIEVEALTEAFQSFTTEKQYCALGSVKSNIGHLLTAAGIAGLIKVL
                     LAIKHRTLPPTIHYDQLNEHIDLADSPFFISKNKEPWTTRQGKPRSAAVSSFGFSGTNA
                     HMVVEEYLPLEESDTAINYHVDQPVLMVLSAKNDKQLKHYADRMIHFIQKNPAIDLQQM
                     AYTLQVGREPMECRMAFLVHSSEELLQTFRMYRLGQQPSNLVTGTLTRENKKPDKRLNK
                     TFEEGQWHEAALWWIQGGEVDWKLLYGDHLPHRINLPTYPFERKSFWPKKDDKTLIENV
                     KVSPSQMPEPVASLPSKTKQTVSLQEVEQQTLKPDQIQLRALKNMPDWPDHHQEQVLSP
                     VTLYKIDQPIDYHQVSHYAEEDEAALQDMLIDSLVQTLFIEGETVDLDQNFYDIGLDSI
                     LGVEWMRLINKTQGLSLEATTLYDYPTIRELARYILSMQGVDAQPQANSYLTTEQADAD
                     DTALIVELSKSLAEIMFLDPSRIDEDVHFSDLGLDSILGVEWIRVLNKRYRTTIEATNL
                     YDYPTLREFAAWMQKDQVKQSIPSTVEEEPALSPPTLEEILQKVEQGALEVTEAHVLLQ
                     KYNLV"
     aSDomain        complement(3553..3747)
                     /aSDomain="ACP_beta"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18165_ACP_beta.3"
                     /evalue="7.00E-28"
                     /label="MOE71_RS18165_ACP_beta.3"
                     /locus_tag="MOE71_RS18165"
                     /protein_end="1591"
                     /protein_start="1526"
                     /score="88.2"
                     /tool="antismash"
                     /translation="ELSKSLAEIMFLDPSRIDEDVHFSDLGLDSILGVEWIRVLNKRYR
                     TTIEATNLYDYPTLREFAAW"
     aSDomain        complement(3838..4035)
                     /aSDomain="ACP_beta"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18165_ACP_beta.2"
                     /evalue="2.30E-25"
                     /label="MOE71_RS18165_ACP_beta.2"
                     /locus_tag="MOE71_RS18165"
                     /protein_end="1496"
                     /protein_start="1430"
                     /score="80.2"
                     /tool="antismash"
                     /translation="MLIDSLVQTLFIEGETVDLDQNFYDIGLDSILGVEWMRLINKTQG
                     LSLEATTLYDYPTIRELARYI"
     aSModule        complement(3838..6072)
                     /complete
                     /domains="nrpspksdomains_MOE71_RS18165_PKS_KS.1"
                     /domains="nrpspksdomains_MOE71_RS18165_Trans-AT_docking.1"
                     /domains="nrpspksdomains_MOE71_RS18165_ACP_beta.2"
                     /locus_tags="MOE71_RS18165"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(4333..4758)
                     /aSDomain="Trans-AT_docking"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18165_Trans-AT_docking.1
                     "
                     /evalue="5.10E-40"
                     /label="MOE71_RS18165_Trans-AT_docking.1"
                     /locus_tag="MOE71_RS18165"
                     /protein_end="1331"
                     /protein_start="1189"
                     /score="129.3"
                     /tool="antismash"
                     /translation="PVLMVLSAKNDKQLKHYADRMIHFIQKNPAIDLQQMAYTLQVGRE
                     PMECRMAFLVHSSEELLQTFRMYRLGQQPSNLVTGTLTRENKKPDKRLNKTFEEGQWHE
                     AALWWIQGGEVDWKLLYGDHLPHRINLPTYPFERKSFW"
     aSDomain        complement(4807..6072)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18165_PKS_KS.1"
                     /domain_subtypes="Trans-AT-KS"
                     /domain_subtypes="beta-Me"
                     /evalue="3.40E-177"
                     /label="MOE71_RS18165_PKS_KS.1"
                     /locus_tag="MOE71_RS18165"
                     /protein_end="1173"
                     /protein_start="751"
                     /score="581.5"
                     /specificity="transATor: beta-Me"
                     /tool="antismash"
                     /translation="IAIVGISGQFPKADNINAFWENIISGKDCISEIPPSRWPMAHYYD
                     TDPEVPGKSTSKWMGVLEDVDKFDPNFFNIPPVDAIAMDPQQRMFLENCWSCIEDAGIR
                     PSDLSGSQCGVFVGCATSDYGQLFNEQNLNSRMLMGNTTSILAARISYLLNLSGPSLAI
                     ETACSSSLVAVAEACNSLVLGNSDLALAGGVCMMAGPSLHIMTSKAGMLSQQGRCRTFD
                     QQADGFVPGEGVGVVLLKRLSDAIRDKDTIYGVIKGWGVNQDGKTNGITAPNGQAQTDL
                     EKSVYEKFGIDPADISLVEAHGTGTKLGDPIEVEALTEAFQSFTTEKQYCALGSVKSNI
                     GHLLTAAGIAGLIKVLLAIKHRTLPPTIHYDQLNEHIDLADSPFFISKNKEPWTTRQGK
                     PRSAAVSSFGFSGTNAHMVVEEY"
     CDS_motif       complement(5134..5178)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS18165_0003"
                     /evalue="2.00E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="MOE71_RS18165"
                     /protein_end="1064"
                     /protein_start="1049"
                     /score="13.4"
                     /tool="antismash"
                     /translation="VEAHGTGTKLGDPIE"
     CDS_motif       complement(5374..5412)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS18165_0002"
                     /evalue="1.60E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="MOE71_RS18165"
                     /protein_end="984"
                     /protein_start="971"
                     /score="11.7"
                     /tool="antismash"
                     /translation="FDQQADGFVPGEG"
     CDS_motif       complement(5554..5601)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_MOE71_RS18165_0001"
                     /evalue="4.60E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="MOE71_RS18165"
                     /protein_end="924"
                     /protein_start="908"
                     /score="18.6"
                     /tool="antismash"
                     /translation="GPSLAIETACSSSLVA"
     aSDomain        complement(7678..7878)
                     /aSDomain="ACP_beta"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MOE71_RS18165_ACP_beta.1"
                     /evalue="5.00E-29"
                     /label="MOE71_RS18165_ACP_beta.1"
                     /locus_tag="MOE71_RS18165"
                     /protein_end="216"
                     /protein_start="149"
                     /score="91.9"
                     /tool="antismash"
                     /translation="EELADSLAEALYIEREMILPGKKFIELGLDSIVGVEWIKTLNKTY
                     GIAIKATKVYDYPTLLDFTQYV"
ORIGIN
        1 attcaaagaa cagggtttta gtcaatgggc caaagaaccc ttccaactgc tgggtcaatt
       61 ttaataccat aatggagtta ataccatact tctctaagtc tatatcgctg gctatttcct
      121 cggcattgat cttaaacacc gaggaaaggt gctcttttaa atagttaatg gttttttccc
      181 gcagacgctg atccccttga gatgatttag cagcctgtaa aaactgagct agatcatcca
      241 gtgtgttctg ttccgctaaa tccttagtgg ttaactctag tccaaactta gtatttagcc
      301 gattcgccaa ttgattcagc tttaaagggt caaatccttg ttctcccaat aggacatcat
      361 gatggatctc gttggcgtca atagccaaca cttctgcagc tatttccttt aacgccggca
      421 ataaatcgtt agccgcgtga ttcttctcct tatgtttgcc ccattgctta aattgccatg
      481 aagcttcctc tggtgtaatc tccttttcct ttaactgccg atagatatgt ttccactgtc
      541 catccatttt tcctctcccc ttatcttgat gccattaaaa tccgctcgac ttcttcttgc
      601 gataattcac cattccaaat tctctccgat agatggcgat aaaaagcttc ttctgacatt
      661 agtcccgctc cccgccgcgt ttcaaagcct aaaatacgta cacataccat gccattctca
      721 tcgtagacat cgatatcaaa tacccgtcct ttctcccttt gccggatgta ggcccacacc
      781 tgtttagggc acggctgata gatatacatt tctttaagag taaaaggcag actatctaaa
      841 ggcgcattta atgaaatagc aaaacctgcc gtcgcttgaa aaacagcatc cagtaggccc
      901 ggatcaaggt agcattccct atctccgcgt gattccggca gtactgatat cttcgccaaa
      961 atctgatcct tatggagata aagacgttca atcccactat gggagggacc ataatatagg
     1021 cccgcttcac gaaaaagcgg atagatattc tccggtaaga gggtgcgcca aggatactga
     1081 gattttaact gttgaaggct ttgtctttta gggggggtta actcagatac cttcaaaaca
     1141 ccttgggcat gcaatatagc tgattgatca tcgggatgat ccgatccgct gtagatttca
     1201 tattctacat tcccattctc tattagtgcc aaggtaatat gcacttcaac cggtttatct
     1261 tccaccacca acggtgcaat ccatatatgc tcttgtaagc aaataaagac cgtgccttgt
     1321 tgtaaagtat cggtagttac ccgatccacc ccttgctcaa gggccgttct tgccatttcc
     1381 agataggctg atccaggtaa cacccttctc tccttcacct ggtgatcagt taaataggct
     1441 tcctttcctg tcaggatggt cgtaaaccgc tgagtcgaca gatcagatgt attgagatga
     1501 actaaagggt gcaaggtact gttacttgag gatgttgtgt cctgcttagg aagccaataa
     1561 tgttccttgg caaaaggata gcccggcaga cggatatgtc gtggctcgtc atccttataa
     1621 aaaacctgcc aatctaccca ttcaccggcc agccactgtg agaaaagtgt ttcaggatca
     1681 gtatgtagag atgtatttct ctgacccttc cgctttttta catggctgat gtgtaaccca
     1741 tccggttgtt catcattcag ataagccgtc aacatatcga tggctgcaaa tttgctatcc
     1801 atccaaaagg ccatgcggta atccatggcc tcccgactcg tctgtaatgt gtaggtcata
     1861 tctttgagat tcacatccac ttgtgatgca atccattctt tcattatgcc cgcatatact
     1921 ttaagttgtt tctccgtttt agcagataaa acaaataaag ccggccggct ggcatctgtt
     1981 actggcatcc gcactctttt tttctttgtt tcgtaggctt ctataaccaa atgagcattg
     2041 gtgccgctaa aaccaaatga actgacacaa gctcgtcttg gtgaatttgc atcccaagga
     2101 cgagtctgtg tattgacata aaaaggggag tttttaaaat caaagtattc atttggctct
     2161 ttaaagtgaa gagtgggcac caactgctga tgctgcatac ttaacaacac tttatgaacg
     2221 ctggctacac cagcggcggc tgatgtgtga ccgatattgg atttgacgga accaatggcg
     2281 caaaactgct ttttatcggt tttttcttga aatgcagttg ataatgcttt aagttcaata
     2341 ggatcaccta attgggtacc cgtgccatgc atttctgcat agctaatgct tgaaggatca
     2401 atgttatagt tttggtagat acttcgctcc aaggctatct ggcttttcac actaggagca
     2461 gttagaccat tcgtttttcc atcttgatta ataccaaatc caataatcac gccataaatt
     2521 aagtcattat cagcctcagc atcctggaga cgctttaaca cgacagcacc aacgccttca
     2581 ccgggaacca tgccatcact ggatttatca aaagccttgc atcgcccctc tggtgaaagc
     2641 atgtgtgccg cgcacatttc aatatatggc ttgggggtga ggtacaacgt tacccctccc
     2701 gcaagagcca tatcaatttt ccggttaagt aacgcttcac acgcaaggtt gatcgctacc
     2761 aaagaggacg aacatgcagt atcaatagaa agcgccggac cttttagatt aagaaagtag
     2821 gcaatacgcg ccgatgcaat agaagcataa ttgccagtca tatcagttaa gccatattgt
     2881 tgttcacaca acatccgata atattcattc gccatgatgc caagataaac tccgcaattc
     2941 tgaccattca atgcctctgg cgtatagccg gcatctgcaa aagcctgata accttcctga
     3001 agaaagagtc gttgttgcgg atccatggtt tctgcctctg aaggcgcaat attgaaaaac
     3061 aggggatcaa agcaggccac atcatccatc aaacctagcc acttactgta tattttccct
     3121 tcatcactgg gaaacggttg atagaatgaa gcaacatccc aacgtgaagg cggtatttcc
     3181 ctgatagaat cgcggcctgc tttaagattt gaccaatagg catcgagatt gtccgcttct
     3241 ggatatctcc ctgacatccc aatgatggca atggcctccc ggtgttcgcc agacttagga
     3301 ttcgtcaatt ggctttttgc ctcttcagat gatatctttc ccgcttgcag ggctcgtaag
     3361 atggcttgtg aatttaccat ttcccttccc cccttaaacc agattgtatt tttgtaacag
     3421 gacatgcgcc tccgttactt ccaatgcgcc ctgctctact ttttgcaaaa tctcttccaa
     3481 tgtgggcggg gatagcgccg gttcctcttc cacagtggat ggaatactct gtttgacttg
     3541 atctttttgc atccaagcgg caaactcgcg taaggtcgga tagtcatata agttcgttgc
     3601 ctcgattgtg gttcgatagc gtttatttaa cacccgaatc cattccacac ctaaaataga
     3661 gtctagaccc aagtctgaaa aatggacatc ttcatcaatc cgactgggat caaggaacat
     3721 aatttcagcc agacttttcg acaactcgac tatcaaagcc gtgtcatctg catccgcttg
     3781 ttctgttgtt aaataggagt tcgcttgtgg ctgtgcatcc accccctgca tagataggat
     3841 ataccgtgcc aattcacgaa tggtcggata atcgtacagt gtggtagcct ccagtgatag
     3901 cccctgcgtc ttattaataa gacgcatcca ttccacgccc aatatagagt ctaagcctat
     3961 atcataaaag ttttgatcca aatccaccgt ttccccttca ataaataagg tctgaaccag
     4021 actatcgatc aacatgtctt gaagtgctgc ctcatcttct tccgcataat gagacacctg
     4081 atggtaatca ataggctggt caatcttgta taaagtgact ggtgataata cttgctcttg
     4141 atggtgatcc ggccaatccg gcatgttctt caatgctctt aattgaatct gatccggctt
     4201 taacgtctgt tgttcaacct cttgcaagct aactgtctgt ttagttttag atggtagact
     4261 ggccaccggc tctggcatct gcgatggact aactttaacg ttttcaatca atgtcttatc
     4321 gtcttttttc ggccaaaaac ttttacgttc aaagggatac gtaggcaaat taatacggtg
     4381 tggcagatgg tctccataca acagtttcca gtcaacctcg ccgccttgta tccaccaaag
     4441 ggctgcctcg tgccattgtc cttcttcaaa agtcttgtta agacgcttgt ctggcttctt
     4501 gttttccctg gttaaggtgc ctgtcaccag gttggacggt tgttgaccta agcgatacat
     4561 tctaaaggtt tgcagcagct cttccgatga atgaaccaag aacgccatcc ggcactccat
     4621 cggctcccgt ccaacctgca aggtataggc catctgttgc aggtcgatgg cgggattctt
     4681 ttggataaaa tggatcatcc gatcggcata gtgtttcagt tgtttgtcgt ttttggctga
     4741 aagcaccatt aatactggtt gatccacatg ataattgatg gcagtatccg attcttccag
     4801 tggtaaatac tcttccacca ccatatgagc attggttcca ctgaagccaa aggaactgac
     4861 ggcggcactg cgtggctttc cctgacgcgt tgtccaaggc tctttgtttt tgctgataaa
     4921 aaagggacta tctgcaaggt ctatgtgctc atttaactga tcatagtgga tcgttggtgg
     4981 cagagtccgg tgtttaatag ccaaaagcac tttaatcaat cccgcaatcc ctgctgctgt
     5041 cagcaaatgc ccgatattac ttttaacaga gcccagtgcg caatactgtt tttcagtagt
     5101 aaaagattgg aaggcttctg tcagtgcttc cacttcaatg ggatcaccca atttggttcc
     5161 cgttccatgg gcctcaacca gtgatatatc agctggatcg attccaaatt tttcataaac
     5221 ggatttttcc agatctgttt gtgcttgccc attaggtgcc gttataccgt tggtttttcc
     5281 atcctgattg acaccccaac cctttatgac accatagatc gtatccttat ctcgaatagc
     5341 atcgctaaga cgttttaata ggaccacacc cactccttcg ccaggaacaa atccatccgc
     5401 ttgttgatca aatgtccggc aacgcccctg ttgggacagc atccccgcct tactggtcat
     5461 tatatggaga gacggacctg ccatcataca aacccctcct gcaagggcca aatcactatt
     5521 gcccaatacg aggctgttac aagcctctgc aaccgctacc aatgaagatg aacatgcagt
     5581 ttcaatcgcc aaggatgggc cagataaatt gagcagataa gaaattcggg ctgccagaat
     5641 agatgtcgtg ttgcccatga gcatacggga atttaagttt tgttcattaa ataattgccc
     5701 ataatcactg gttgcacatc caacaaatac accacattgg cttcctgaaa ggtcagatgg
     5761 ccgaatgcct gcatcctcta tacaactcca gcaattctcg agaaacatcc tttgctgtgg
     5821 atccattgcg atggcgtcca ctggtgggat attaaagaaa ttcggatcaa atttgtccac
     5881 atcctctaaa actcccatcc atttgctagt actcttcccc ggaacttctg gatcggtatc
     5941 gtagtagtgt gccattggcc atcgggaagg tggaatctct gaaatacaat ctttcccact
     6001 gataatattt tcccaaaatg cgttgatatt atcagcttta ggaaactgcc cagatatacc
     6061 cacaatcgca atatccgtct ctttaagacg cggatcctca atagctcgtt gctggattga
     6121 cggtgtctcg acagcaacag ctcgagggct atatcgggtt tgcaggtgtt cgcaagcctg
     6181 ctccatcagc aagacggcga catctaccac atgacgcttt ttccagtctt cgtatggcgt
     6241 gccctttacc cattgattaa acgcccctaa ggcgggacca caaaatattt gaaagttatc
     6301 tttttcagtt aaatcgccgt taagtgtggc ccggttacaa taggcgaaat accatttaaa
     6361 aataagcgcc atcttaaagc gaggattgac actcgcttct gttaattgct gagggttttt
     6421 ctttttcttg tattcgcaaa ctaaatccca gacttcggcg aaggtgcgct taaaataatt
     6481 gatttcaatt tcctgtttaa tctcttgtgg tatttcttcg atggcattat actgcataaa
     6541 cagctcatac agcctattag cccggttata aaactgggta tgtttacgga ccacttgggc
     6601 tttcgcacca atctcaaaca tatcacctgc aactgtcaca gccgtatcat gaatggaaat
     6661 ggtgctaagc aactctttta ttacatcgtg tgcgccactt tcaaccgtac actggttaat
     6721 ggagccagtc aaaacaaagt cagctcccat cataaaggct gatgccaccg cctctggagt
     6781 accaatccct ccaccgcatc ctacaaggat gggttccgga tatgagtagg tttgctgtat
     6841 atcctttcgc agggctagca cagaagggac aagagcagac agcacgcctc gatcggtatg
     6901 accgcctgag tccgcctcta aaacaatatc atcaaccatt ggaatagatc gggataatac
     6961 cgcctcttct gctgtgatca agccttcttc caacagttga tccactaaat cctctggcgg
     7021 aggcgccata aattgtttgg ctatctccaa acgagagcat tttcctatga ttcttcttgg
     7081 aaaatagagg atgccatcct tctgcttttt aatccctttg acacggcaat aaaccaatgg
     7141 tgccgttatg cttgaaaagg cagatgcttc aataaaggga acgccatggg ttataaatat
     7201 ctgggcttgc tcccactctt cttccaggtt attcacattg gcgattaggc acatgccata
     7261 aggcttattg ggtcctacaa cagattgaat atgttggata tgtgacgcca acctgtctcc
     7321 atccaagccg gcgctgccaa aaaagctaag caagttagct tctgccatgg cgatcaccaa
     7381 tgcttctgaa gcgacccctt gagccattga acccgccata tagttccatt tacaacggta
     7441 gcgggtttga aacaatttgt ctcccaggct ttccggacgg atcacattct tctttactcc
     7501 tggacttgcc ggcttctgat taagcaatgc agggttctta ttcccatgaa tggaagtggg
     7561 agacgcatct attttcataa tcggtgcctt taaagtctct ttcgtatccc cttttccttc
     7621 agctgacaaa ggtttctgtt tatttggtcg tttttttaaa cggcccaact cactcgcaac
     7681 atattgtgta aagtcaagta gtgtcggata atcatacacc ttggtggctt taatggctat
     7741 tccataggtt ttatttaagg tcttaatcca ctcaactcca acgatagaat ctaggcccaa
     7801 ttcaataaat tttttacctg gcagaatcat ctcacgctca atatataaag cttctgctaa
     7861 actgtccgcc aattcttcct gcaattcttc caacccctgc tcctcgtaag actcctcttc
     7921 tatgctatct tgtttagaat ctggcaacac catctcaaca ggagatgaag cggggatact
     7981 tatggtttta tcatttgcga caacagataa tgcggttgca atttcaggct gtgaagaatc
     8041 gactacaggt gtctgttccg gttctgacgc tttgggtgcc caataggaat tcctcgcaaa
     8101 cggataagtg ggcaaatgaa cccgttcagg ctcttccttc cacatttgtt tccaaggtaa
     8161 atcgtagcct tggcagtaaa ggttagacag accttgcaaa atttcctgat attgctctga
     8221 tgttgccgca ttatggctct gctgaatgag gtcacttcca aacaactcca tgaccttttg
     8281 actcataaat ccttctgata cctcaccgct atagctattt ggcaagtgct ccccgccttt
     8341 aaactgtttc agcaggtata aagcttccgt ctggtctttg accaccacta catatcgatg
     8401 acgaaaatgc tggc
//
